FvH4_1g02212

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000658
Physical Location & Seq
Reverse (-)
1189367 .. 1189971
605 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g02212.t1

Sequence Viewer

Length: 528 bp
ATGTTTTATGAAGACGATGATTCAAAAGAATCAAAATTAAAAGCTCATATGGCGTTTCTAAAGCTGTTATTTCGTCCAATCCGTCTTGCAACAAGAACTTTATTGTGCATGGTAGTGAAATATGGTTGCGATACACTAGCATTCAATAAACATGGAGACGAAGACATGTCCTGGAAAAGGTTAGAGGATGGTAAGCATTTTTATCAAAAACATCTTGTGTTTCATGATGACCATTTAGTTGCAGTGACATGGAGGGGTCATACAAAGGTTTGGGACTTTAAGGAATACCCACCTTTACACCTTTACAACGTAGCCCTATTGAAGAGTGAAGACCCTGCTGCCCGCAGATCTGGTAGTGGTACCTATTTGATGGAGTCATTATCGGGTGAGATCTTACGTCTGAAACAAAGTAAATTCAAGGATGAAAGTTGGTCCTTCAATTTACACTTTTTTGTGGAAAAGTTTAGCAGAAGGCAAATGGGAAAATTTGCAGAGTTTAGGCTATCAATGTTTATTCTTGTCTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

176

Amino Acids

20.79

Weight (kDa)

9.15

Isoelectric Point (pI)

42.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 30 - 149 3.4e-09 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 359
AccB1I GGYRCC 1 cut(s) 359
AciI CCGC 1 cut(s) 343
AcsI RAATTY 2 cut(s) 413, 485
AfaI GTAC 1 cut(s) 361
AfiI CCNNNNNNNGG 1 cut(s) 177
AflIII ACRYGT 1 cut(s) 165
AgsI TTSAA 5 cut(s) 24, 145, 322, 418, 439
AjnI CCWGG 1 cut(s) 170
AluBI AGCT 2 cut(s) 44, 64
AluI AGCT 2 cut(s) 44, 64
Alw26I GTCTC 1 cut(s) 150
ApeKI GCWGC 1 cut(s) 338
ApoI RAATTY 2 cut(s) 413, 485
Asp718I GGTACC 1 cut(s) 359
AspS9I GGNCC 1 cut(s) 432
AsuHPI GGTGA 1 cut(s) 398
AvaII GGWCC 1 cut(s) 432
BanI GGYRCC 1 cut(s) 359
BbsI GAAGAC 3 cut(s) 18, 168, 336
BbvI GCAGC 1 cut(s) 325
BccI CCATC 2 cut(s) 182, 364
BciT130I CCWGG 1 cut(s) 172
BcoDI GTCTC 1 cut(s) 150
BfaI CTAG 1 cut(s) 137
BglII AGATCT 2 cut(s) 347, 390
BisI GCNGC 1 cut(s) 339
BlsI GCNGC 1 cut(s) 340
Bme1390I CCNGG 1 cut(s) 172
Bme18I GGWCC 1 cut(s) 432
BmgT120I GGNCC 1 cut(s) 432
BmiI GGNNCC 1 cut(s) 361
BmrFI CCNGG 1 cut(s) 172
BpiI GAAGAC 3 cut(s) 18, 168, 336
Bsc4I CCNNNNNNNGG 1 cut(s) 177
BseBI CCWGG 1 cut(s) 172
BseGI GGATG 2 cut(s) 193, 427
BseLI CCNNNNNNNGG 1 cut(s) 177
BseXI GCAGC 1 cut(s) 325
BshNI GGYRCC 1 cut(s) 359
BslFI GGGAC 1 cut(s) 287
BslI CCNNNNNNNGG 1 cut(s) 177
BsmAI GTCTC 1 cut(s) 150
BsmBI CGTCTC 1 cut(s) 150
BsmFI GGGAC 1 cut(s) 287
BsmI GAATGC 1 cut(s) 140
Bsp143I GATC 2 cut(s) 347, 390
BspACI CCGC 1 cut(s) 343
BspHI TCATGA 1 cut(s) 223
BspLI GGNNCC 1 cut(s) 361
BspT107I GGYRCC 1 cut(s) 359
BssMI GATC 2 cut(s) 347, 390
Bst2UI CCWGG 1 cut(s) 172
Bst6I CTCTTC 1 cut(s) 317
BstC8I GCNNGC 1 cut(s) 343
BstENI CCTNNNNNAGG 1 cut(s) 175
BstF5I GGATG 2 cut(s) 193, 427
BstKTI GATC 2 cut(s) 350, 393
BstMAI GTCTC 1 cut(s) 150
BstMBI GATC 2 cut(s) 347, 390
BstMWI GCNNNNNNNGC 1 cut(s) 50
BstNI CCWGG 1 cut(s) 172
BstNSI RCATGY 1 cut(s) 169
BstSCI CCNGG 1 cut(s) 170
BstV1I GCAGC 1 cut(s) 325
BstV2I GAAGAC 3 cut(s) 18, 168, 336
BstX2I RGATCY 2 cut(s) 347, 390
BstYI RGATCY 2 cut(s) 347, 390
BtsCI GGATG 2 cut(s) 193, 427
BtsI GCAGTG 1 cut(s) 249
BtsIMutI CAGTG 1 cut(s) 249
Cac8I GCNNGC 1 cut(s) 343
CciI TCATGA 1 cut(s) 223
Cfr13I GGNCC 1 cut(s) 432
Csp6I GTAC 1 cut(s) 360
CviAII CATG 5 cut(s) 109, 152, 166, 224, 249
CviJI RGCY 4 cut(s) 44, 64, 314, 502
CviKI_1 RGCY 4 cut(s) 44, 64, 314, 502
CviQI GTAC 1 cut(s) 360
DpnI GATC 2 cut(s) 349, 392
DpnII GATC 2 cut(s) 347, 390
Eam1104I CTCTTC 1 cut(s) 317
EarI CTCTTC 1 cut(s) 317
Eco47I GGWCC 1 cut(s) 432
EcoNI CCTNNNNNAGG 1 cut(s) 175
EcoRII CCWGG 1 cut(s) 170
Esp3I CGTCTC 1 cut(s) 150
FaeI CATG 5 cut(s) 112, 155, 169, 227, 252
FaqI GGGAC 1 cut(s) 287
FatI CATG 5 cut(s) 108, 151, 165, 223, 248
FauI CCCGC 1 cut(s) 350
FauNDI CATATG 1 cut(s) 48
Fnu4HI GCNGC 1 cut(s) 339
FokI GGATG 2 cut(s) 200, 434
Fsp4HI GCNGC 1 cut(s) 339
FspBI CTAG 1 cut(s) 137
GluI GCNGC 1 cut(s) 339
Hin1II CATG 5 cut(s) 112, 155, 169, 227, 252
HinfI GANTC 3 cut(s) 20, 29, 374
HphI GGTGA 1 cut(s) 398
Hpy188I TCNGA 1 cut(s) 402
Hpy188III TCNNGA 1 cut(s) 224
HpyAV CCTTC 2 cut(s) 445, 465
HpyCH4IV ACGT 2 cut(s) 309, 397
HpyCH4V TGCA 4 cut(s) 89, 108, 242, 491
HpyF10VI GCNNNNNNNGC 1 cut(s) 50
HpySE526I ACGT 2 cut(s) 309, 397
Hsp92II CATG 5 cut(s) 112, 155, 169, 227, 252
KpnI GGTACC 1 cut(s) 363
Kzo9I GATC 2 cut(s) 347, 390
LpnPI CCDG 4 cut(s) 157, 184, 336, 348
Lsp1109I GCAGC 1 cut(s) 325
MaeI CTAG 1 cut(s) 137
MaeII ACGT 2 cut(s) 309, 397
MaeIII GTNAC 1 cut(s) 244
MalI GATC 2 cut(s) 349, 392
MboI GATC 2 cut(s) 347, 390
MboII GAAGA 4 cut(s) 23, 173, 334, 341
MflI RGATCY 2 cut(s) 347, 390
MluCI AATT 4 cut(s) 35, 413, 439, 485
MlyI GAGTC 1 cut(s) 383
MnlI CCTC 2 cut(s) 178, 246
MseI TTAA 2 cut(s) 38, 279
MslI CAYNNNNRTG 1 cut(s) 113
MspR9I CCNGG 1 cut(s) 172
Mva1269I GAATGC 1 cut(s) 140
MvaI CCWGG 1 cut(s) 172
MwoI GCNNNNNNNGC 1 cut(s) 50
NdeI CATATG 1 cut(s) 48
NdeII GATC 2 cut(s) 347, 390
NlaIII CATG 5 cut(s) 112, 155, 169, 227, 252
NlaIV GGNNCC 1 cut(s) 361
NmuCI GTSAC 1 cut(s) 244
NspI RCATGY 1 cut(s) 169
PagI TCATGA 1 cut(s) 223
PciI ACATGT 1 cut(s) 165
PcsI WCGNNNNNNNCGW 1 cut(s) 79
PctI GAATGC 1 cut(s) 140
PfeI GAWTC 2 cut(s) 20, 29
PfoI TCCNGGA 1 cut(s) 170
PkrI GCNGC 1 cut(s) 340
PleI GAGTC 1 cut(s) 382
PpsI GAGTC 1 cut(s) 382
PscI ACATGT 1 cut(s) 165
Psp6I CCWGG 1 cut(s) 170
PspGI CCWGG 1 cut(s) 170
PspN4I GGNNCC 1 cut(s) 361
PspPI GGNCC 1 cut(s) 432
PsuI RGATCY 2 cut(s) 347, 390
RsaI GTAC 1 cut(s) 361
RsaNI GTAC 1 cut(s) 360
RseI CAYNNNNRTG 1 cut(s) 113
SaqAI TTAA 2 cut(s) 38, 279
SatI GCNGC 1 cut(s) 339
Sau3AI GATC 2 cut(s) 347, 390
Sau96I GGNCC 1 cut(s) 432
SchI GAGTC 1 cut(s) 383
ScrFI CCNGG 1 cut(s) 172
SetI ASST 9 cut(s) 46, 66, 182, 270, 295, 303, 312, 365, 400
SinI GGWCC 1 cut(s) 432
SmiMI CAYNNNNRTG 1 cut(s) 113
Sse9I AATT 4 cut(s) 35, 413, 439, 485
SsiI CCGC 1 cut(s) 343
SspMI CTAG 1 cut(s) 137
StyD4I CCNGG 1 cut(s) 170
TaiI ACGT 2 cut(s) 312, 400
TasI AATT 4 cut(s) 35, 413, 439, 485
TfiI GAWTC 2 cut(s) 20, 29
Tru1I TTAA 2 cut(s) 38, 279
Tru9I TTAA 2 cut(s) 38, 279
TscAI CASTG 1 cut(s) 249
TseFI GTSAC 1 cut(s) 244
TseI GCWGC 1 cut(s) 338
Tsp45I GTSAC 1 cut(s) 244
TspDTI ATGAA 3 cut(s) 24, 212, 438
TspGWI ACGGA 1 cut(s) 71
TspRI CASTG 1 cut(s) 249
VpaK11BI GGWCC 1 cut(s) 432
XagI CCTNNNNNAGG 1 cut(s) 175
XapI RAATTY 2 cut(s) 413, 485
XceI RCATGY 1 cut(s) 169
XspI CTAG 1 cut(s) 137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.