AT4G22180

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0000658
Physical Location & Seq
Forward (+)
11738511 .. 11739782
1272 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G22180.1

Sequence Viewer

Length: 1209 bp
ATGGAAAAGTGGCATAACCCTAGTTCTCCTAAGCGTCTCAGAGGAGATACACCCAATTCCTGGTCGGAGCTTCCTCTAGATCTCTTGACTGCAGTGTTCGAACGCCTCAGCTATGCAAATTTCCAACGAGCTAAATCCGTCTGTTCGTCTTGGCACTCCGGATCGAGACAATCCGTTCCCATACAGATCCCTTGGCTGATTCTCTTCCCGGAATACGACAACAACAATTCATGCACGTTGTTCAATCCCGAGGAAAAAGGCCAAGTTTACAAAATGAAAGATCTTGGTGTTGAATTTTCAAAGAGTGTTTGTACTGCAACCTACGGAAGCTGGCTCCTTATGCGAGATCCACTGTATAATCTCTATATTTTGAATCTATTCACCCACGAGAGGGTAAATCTACCTCCTTTCGAGTCACAACTTGGCATGGTAAAGATTGAACGAACCATATATGATTGGTTTCACTCTACACTCCATTACAACGGTAAGGAGTACCACAAACGTATTAGAATACTATCCACTGTGTTTTGGATCGATGAGAAAACAAAAGATTACGTAGTTATATGGGGACTTGGAAGTTCATGTGTGGTTTATTCCAAGAAAGGAGATAAATGTTGGAATCAAATTCCAGAAACTTCAAATTGTCATCACATGGTTTACAAGGATCACAAGCTTTACTTCTCTACGTCTACCTACAAATATGAATTCAGAATCTTTGATTTCTCTAGAGAGATTCCTCAACAAATCTTTCAAGGCTATGTCATTATGCAAGGACTTACATTAAATCGTCATCGTGGTCAACCAGGTTATCCCTTTGCTACTATTGATACAAAACTTGTTGTCACAGTAACTGGAGATGTCCTTAAGGTTGACAGAATAGTGGAGAAAGAGACTAGAATCTGCAGATTTTTTTACGTCTACAAGGTTTATTCATCGGGGTCCTATAAGAAATATGAAAAAGTTGAATCTTTGGGGGACGAGGCAATTCTTTTGGATCTAGGTATCACTATGCTCGCCAATGACACTGTGGGACTCCTAGGAAATTCCATTTATTTCAGTGGTACTCATACAAAGTCGAAGGTCATTAACGATACATTTATCTTCAGTCTCGAGACACAGAAGATGGATCCAGTGCACAAGTTTGATTGTTCATCAGCTCAACTCTCTAGTGCTCGATGGTTCTTACCAAGTTCACACAAACTTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box

Protein Analysis

402

Amino Acids

46.8

Weight (kDa)

8.68

Isoelectric Point (pI)

39.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 21 - 53 6.5e-06 F-box domain
Beta-prop_KIB1-4 PF03478 81 - 356 7.1e-45 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 60
AccI GTMKAC 2 cut(s) 689, 918
AccIII TCCGGA 1 cut(s) 158
AclWI GGATC 8 cut(s) 169, 181, 341, 539, 672, 1002, 1121, 1134
AcsI RAATTY 5 cut(s) 118, 293, 624, 704, 1042
AcuI CTGAAG 1 cut(s) 1087
AfaI GTAC 3 cut(s) 313, 494, 1063
AfiI CCNNNNNNNGG 3 cut(s) 60, 390, 391
AflII CTTAAG 1 cut(s) 863
AgsI TTSAA 8 cut(s) 244, 293, 300, 373, 440, 639, 752, 965
AjnI CCWGG 2 cut(s) 59, 802
AluBI AGCT 6 cut(s) 70, 111, 131, 330, 673, 1157
AluI AGCT 6 cut(s) 70, 111, 131, 330, 673, 1157
Alw21I GWGCWC 2 cut(s) 1137, 1174
Alw26I GTCTC 5 cut(s) 41, 160, 884, 1106, 1112
Alw44I GTGCAC 1 cut(s) 1133
AlwI GGATC 8 cut(s) 169, 181, 341, 539, 672, 1002, 1121, 1134
AlwNI CAGNNNCTG 1 cut(s) 851
Ama87I CYCGRG 2 cut(s) 248, 1109
Aor13HI TCCGGA 1 cut(s) 158
AoxI GGCC 1 cut(s) 259
ApaLI GTGCAC 1 cut(s) 1133
ApoI RAATTY 5 cut(s) 118, 293, 624, 704, 1042
Asp700I GAANNNNTTC 1 cut(s) 377
AspA2I CCTAGG 1 cut(s) 1036
AspS9I GGNCC 1 cut(s) 939
AsuC2I CCSGG 1 cut(s) 209
AsuHPI GGTGA 1 cut(s) 373
AsuII TTCGAA 1 cut(s) 99
AvaI CYCGRG 2 cut(s) 248, 1109
AvaII GGWCC 1 cut(s) 939
AvrII CCTAGG 1 cut(s) 1036
BaeGI GKGCMC 1 cut(s) 1137
BamHI GGATCC 1 cut(s) 1126
BauI CACGAG 1 cut(s) 386
Bbv12I GWGCWC 2 cut(s) 1137, 1174
BbvCI CCTCAGC 1 cut(s) 107
BccI CCATC 2 cut(s) 1117, 1170
BciT130I CCWGG 2 cut(s) 61, 804
BcnI CCSGG 1 cut(s) 209
BcoDI GTCTC 5 cut(s) 41, 160, 884, 1106, 1112
BfaI CTAG 7 cut(s) 21, 77, 726, 894, 998, 1037, 1167
BfmI CTRYAG 2 cut(s) 90, 901
BfrI CTTAAG 1 cut(s) 863
BglII AGATCT 2 cut(s) 79, 280
BlnI CCTAGG 1 cut(s) 1036
Bme1390I CCNGG 3 cut(s) 61, 209, 804
Bme18I GGWCC 1 cut(s) 939
BmeT110I CYCGRG 2 cut(s) 248, 1109
BmgT120I GGNCC 1 cut(s) 939
BmiI GGNNCC 3 cut(s) 335, 940, 1128
BmrFI CCNGG 3 cut(s) 61, 209, 804
BpmI CTGGAG 1 cut(s) 873
Bpu10I CCTNAGC 2 cut(s) 30, 107
Bpu14I TTCGAA 1 cut(s) 99
BpuMI CCSGG 1 cut(s) 209
Bsa29I ATCGAT 1 cut(s) 534
BsaAI YACGTR 1 cut(s) 556
BsaJI CCNNGG 3 cut(s) 191, 249, 1036
BsaWI WCCGGW 1 cut(s) 158
Bsc4I CCNNNNNNNGG 3 cut(s) 60, 390, 391
Bse1I ACTGG 2 cut(s) 856, 1130
BseAI TCCGGA 1 cut(s) 158
BseBI CCWGG 2 cut(s) 61, 804
BseCI ATCGAT 1 cut(s) 534
BseDI CCNNGG 3 cut(s) 191, 249, 1036
BseLI CCNNNNNNNGG 3 cut(s) 60, 390, 391
BseMII CTCAG 2 cut(s) 52, 121
BseNI ACTGG 2 cut(s) 856, 1130
BseRI GAGGAG 1 cut(s) 57
BseSI GKGCMC 1 cut(s) 1137
BshFI GGCC 1 cut(s) 261
BshVI ATCGAT 1 cut(s) 534
BsiHKAI GWGCWC 2 cut(s) 1137, 1174
BsiHKCI CYCGRG 2 cut(s) 248, 1109
BsiSI CCGG 2 cut(s) 159, 209
BslFI GGGAC 3 cut(s) 582, 989, 1044
BslI CCNNNNNNNGG 3 cut(s) 60, 390, 391
BsmAI GTCTC 5 cut(s) 41, 160, 884, 1106, 1112
BsmBI CGTCTC 1 cut(s) 41
BsmFI GGGAC 3 cut(s) 582, 989, 1044
BsnI GGCC 1 cut(s) 261
BsoBI CYCGRG 2 cut(s) 248, 1109
Bsp119I TTCGAA 1 cut(s) 99
Bsp1286I GDGCHC 2 cut(s) 1137, 1174
Bsp13I TCCGGA 1 cut(s) 158
Bsp143I GATC 9 cut(s) 79, 161, 186, 280, 346, 531, 664, 994, 1126
BspANI GGCC 1 cut(s) 261
BspCNI CTCAG 2 cut(s) 51, 120
BspDI ATCGAT 1 cut(s) 534
BspEI TCCGGA 1 cut(s) 158
BspLI GGNNCC 3 cut(s) 335, 940, 1128
BspMAI CTGCAG 2 cut(s) 94, 905
BspPI GGATC 8 cut(s) 169, 181, 341, 539, 672, 1002, 1121, 1134
BspT104I TTCGAA 1 cut(s) 99
BspTI CTTAAG 1 cut(s) 863
BsrI ACTGG 2 cut(s) 856, 1130
BssECI CCNNGG 3 cut(s) 191, 249, 1036
BssMI GATC 9 cut(s) 79, 161, 186, 280, 346, 531, 664, 994, 1126
BssSI CACGAG 1 cut(s) 386
BssT1I CCWWGG 2 cut(s) 191, 1036
Bst2BI CACGAG 1 cut(s) 386
Bst2UI CCWGG 2 cut(s) 61, 804
Bst4CI ACNGT 5 cut(s) 354, 485, 523, 847, 1027
Bst6I CTCTTC 1 cut(s) 209
BstAFI CTTAAG 1 cut(s) 863
BstBAI YACGTR 1 cut(s) 556
BstBI TTCGAA 1 cut(s) 99
BstC8I GCNNGC 2 cut(s) 332, 1014
BstDEI CTNAG 3 cut(s) 30, 38, 107
BstKTI GATC 9 cut(s) 82, 164, 189, 283, 349, 534, 667, 997, 1129
BstMAI GTCTC 5 cut(s) 41, 160, 884, 1106, 1112
BstMBI GATC 9 cut(s) 79, 161, 186, 280, 346, 531, 664, 994, 1126
BstMWI GCNNNNNNNGC 1 cut(s) 340
BstNI CCWGG 2 cut(s) 61, 804
BstSCI CCNGG 3 cut(s) 59, 207, 802
BstSFI CTRYAG 2 cut(s) 90, 901
BstSLI GKGCMC 1 cut(s) 1137
BstSNI TACGTA 1 cut(s) 556
BstX2I RGATCY 6 cut(s) 79, 186, 280, 346, 994, 1126
BstYI RGATCY 6 cut(s) 79, 186, 280, 346, 994, 1126
Bsu15I ATCGAT 1 cut(s) 534
BsuRI GGCC 1 cut(s) 261
BsuTUI ATCGAT 1 cut(s) 534
BtsI GCAGTG 1 cut(s) 99
BtsIMutI CAGTG 6 cut(s) 99, 350, 519, 1023, 1063, 1137
Cac8I GCNNGC 2 cut(s) 332, 1014
CaiI CAGNNNCTG 1 cut(s) 851
Cfr13I GGNCC 1 cut(s) 939
ClaI ATCGAT 1 cut(s) 534
CseI GACGC 1 cut(s) 23
CsiI ACCWGGT 1 cut(s) 802
Csp6I GTAC 3 cut(s) 312, 493, 1062
CviAII CATG 4 cut(s) 231, 427, 582, 652
CviQI GTAC 3 cut(s) 312, 493, 1062
DdeI CTNAG 3 cut(s) 30, 38, 107
DpnI GATC 9 cut(s) 81, 163, 188, 282, 348, 533, 666, 996, 1128
DpnII GATC 9 cut(s) 79, 161, 186, 280, 346, 531, 664, 994, 1126
Eam1104I CTCTTC 1 cut(s) 209
EarI CTCTTC 1 cut(s) 209
Eco105I TACGTA 1 cut(s) 556
Eco130I CCWWGG 2 cut(s) 191, 1036
Eco47I GGWCC 1 cut(s) 939
Eco57I CTGAAG 1 cut(s) 1087
Eco88I CYCGRG 2 cut(s) 248, 1109
EcoO109I RGGNCCY 1 cut(s) 939
EcoRI GAATTC 1 cut(s) 704
EcoRII CCWGG 2 cut(s) 59, 802
EcoT14I CCWWGG 2 cut(s) 191, 1036
ErhI CCWWGG 2 cut(s) 191, 1036
Esp3I CGTCTC 1 cut(s) 41
FaeI CATG 4 cut(s) 234, 430, 585, 655
FalI AAGNNNNNCTT 2 cut(s) 662, 694
FaqI GGGAC 3 cut(s) 582, 989, 1044
FatI CATG 4 cut(s) 230, 426, 581, 651
FblI GTMKAC 2 cut(s) 689, 918
FspBI CTAG 7 cut(s) 21, 77, 726, 894, 998, 1037, 1167
GsuI CTGGAG 1 cut(s) 873
HaeIII GGCC 1 cut(s) 261
HapII CCGG 2 cut(s) 159, 209
HgaI GACGC 1 cut(s) 23
Hin1II CATG 4 cut(s) 234, 430, 585, 655
HincII GTYRAC 2 cut(s) 800, 871
HindII GTYRAC 2 cut(s) 800, 871
HindIII AAGCTT 1 cut(s) 671
HinfI GANTC 9 cut(s) 199, 373, 413, 619, 711, 733, 897, 965, 1032
HpaII CCGG 2 cut(s) 159, 209
HphI GGTGA 1 cut(s) 373
Hpy166II GTNNAC 8 cut(s) 268, 658, 690, 800, 871, 919, 1135, 1193
Hpy188I TCNGA 3 cut(s) 41, 67, 710
Hpy188III TCNNGA 9 cut(s) 77, 85, 159, 165, 248, 629, 726, 1109, 1111
Hpy8I GTNNAC 8 cut(s) 268, 658, 690, 800, 871, 919, 1135, 1193
HpyAV CCTTC 1 cut(s) 1072
HpyCH4III ACNGT 5 cut(s) 354, 485, 523, 847, 1027
HpyCH4IV ACGT 5 cut(s) 236, 502, 555, 686, 915
HpyCH4V TGCA 7 cut(s) 92, 116, 234, 317, 769, 903, 1135
HpyF10VI GCNNNNNNNGC 1 cut(s) 340
HpyF3I CTNAG 3 cut(s) 30, 38, 107
HpySE526I ACGT 5 cut(s) 236, 502, 555, 686, 915
Hsp92II CATG 4 cut(s) 234, 430, 585, 655
Kpn2I TCCGGA 1 cut(s) 158
Kzo9I GATC 9 cut(s) 79, 161, 186, 280, 346, 531, 664, 994, 1126
LmnI GCTCC 2 cut(s) 67, 339
MabI ACCWGGT 1 cut(s) 802
MaeI CTAG 7 cut(s) 21, 77, 726, 894, 998, 1037, 1167
MaeII ACGT 5 cut(s) 236, 502, 555, 686, 915
MaeIII GTNAC 3 cut(s) 414, 841, 847
MalI GATC 9 cut(s) 81, 163, 188, 282, 348, 533, 666, 996, 1128
MboI GATC 9 cut(s) 79, 161, 186, 280, 346, 531, 664, 994, 1126
MboII GAAGA 3 cut(s) 196, 1093, 1132
MflI RGATCY 6 cut(s) 79, 186, 280, 346, 994, 1126
MhlI GDGCHC 2 cut(s) 1137, 1174
MluCI AATT 9 cut(s) 55, 118, 226, 293, 624, 640, 704, 984, 1042
MlyI GAGTC 2 cut(s) 422, 1026
MmeI TCCRAC 3 cut(s) 45, 148, 596
MnlI CCTC 8 cut(s) 35, 84, 116, 244, 384, 414, 747, 973
MroI TCCGGA 1 cut(s) 158
MroXI GAANNNNTTC 1 cut(s) 377
MseI TTAA 3 cut(s) 782, 864, 1086
MspCI CTTAAG 1 cut(s) 863
MspI CCGG 2 cut(s) 159, 209
MspR9I CCNGG 3 cut(s) 61, 209, 804
MvaI CCWGG 2 cut(s) 61, 804
MwoI GCNNNNNNNGC 1 cut(s) 340
NciI CCSGG 1 cut(s) 209
NdeII GATC 9 cut(s) 79, 161, 186, 280, 346, 531, 664, 994, 1126
NlaIII CATG 4 cut(s) 234, 430, 585, 655
NlaIV GGNNCC 3 cut(s) 335, 940, 1128
NmuCI GTSAC 2 cut(s) 414, 841
NspV TTCGAA 1 cut(s) 99
PaeR7I CTCGAG 1 cut(s) 1109
PdmI GAANNNNTTC 1 cut(s) 377
PfeI GAWTC 7 cut(s) 199, 373, 619, 711, 733, 897, 965
PflMI CCANNNNNTGG 1 cut(s) 60
PfoI TCCNGGA 1 cut(s) 207
PleI GAGTC 2 cut(s) 421, 1026
PpsI GAGTC 2 cut(s) 421, 1026
Ppu21I YACGTR 1 cut(s) 556
PpuMI RGGWCCY 1 cut(s) 939
Psp5II RGGWCCY 1 cut(s) 939
Psp6I CCWGG 2 cut(s) 59, 802
PspGI CCWGG 2 cut(s) 59, 802
PspN4I GGNNCC 3 cut(s) 335, 940, 1128
PspPI GGNCC 1 cut(s) 939
PspPPI RGGWCCY 1 cut(s) 939
PstI CTGCAG 2 cut(s) 94, 905
PstNI CAGNNNCTG 1 cut(s) 851
PsuI RGATCY 6 cut(s) 79, 186, 280, 346, 994, 1126
RsaI GTAC 3 cut(s) 313, 494, 1063
RsaNI GTAC 3 cut(s) 312, 493, 1062
SaqAI TTAA 3 cut(s) 782, 864, 1086
Sau3AI GATC 9 cut(s) 79, 161, 186, 280, 346, 531, 664, 994, 1126
Sau96I GGNCC 1 cut(s) 939
SchI GAGTC 2 cut(s) 422, 1026
ScrFI CCNGG 3 cut(s) 61, 209, 804
SduI GDGCHC 2 cut(s) 1137, 1174
SexAI ACCWGGT 1 cut(s) 802
SfcI CTRYAG 2 cut(s) 90, 901
Sfr274I CTCGAG 1 cut(s) 1109
SfuI TTCGAA 1 cut(s) 99
SinI GGWCC 1 cut(s) 939
SlaI CTCGAG 1 cut(s) 1109
SmlI CTYRAG 2 cut(s) 863, 1109
SmoI CTYRAG 2 cut(s) 863, 1109
SnaBI TACGTA 1 cut(s) 556
Sse9I AATT 9 cut(s) 55, 118, 226, 293, 624, 640, 704, 984, 1042
SspMI CTAG 7 cut(s) 21, 77, 726, 894, 998, 1037, 1167
StyD4I CCNGG 3 cut(s) 59, 207, 802
StyI CCWWGG 2 cut(s) 191, 1036
TaaI ACNGT 5 cut(s) 354, 485, 523, 847, 1027
TaiI ACGT 5 cut(s) 239, 505, 558, 689, 918
TaqI TCGA 7 cut(s) 99, 164, 411, 534, 1076, 1110, 1174
TasI AATT 9 cut(s) 55, 118, 226, 293, 624, 640, 704, 984, 1042
TatI WGTACW 1 cut(s) 311
TfiI GAWTC 7 cut(s) 199, 373, 619, 711, 733, 897, 965
Tru1I TTAA 3 cut(s) 782, 864, 1086
Tru9I TTAA 3 cut(s) 782, 864, 1086
TscAI CASTG 6 cut(s) 99, 357, 526, 1030, 1063, 1137
TseFI GTSAC 2 cut(s) 414, 841
Tsp45I GTSAC 2 cut(s) 414, 841
TspDTI ATGAA 7 cut(s) 219, 290, 570, 717, 921, 969, 1140
TspGWI ACGGA 3 cut(s) 127, 163, 339
TspRI CASTG 6 cut(s) 99, 357, 526, 1030, 1063, 1137
Van91I CCANNNNNTGG 1 cut(s) 60
Vha464I CTTAAG 1 cut(s) 863
VneI GTGCAC 1 cut(s) 1133
VpaK11BI GGWCC 1 cut(s) 939
XapI RAATTY 5 cut(s) 118, 293, 624, 704, 1042
XbaI TCTAGA 2 cut(s) 76, 725
XcmI CCANNNNNNNNNTGG 1 cut(s) 1024
XhoI CTCGAG 1 cut(s) 1109
XmaJI CCTAGG 1 cut(s) 1036
XmiI GTMKAC 2 cut(s) 689, 918
XmnI GAANNNNTTC 1 cut(s) 377
XspI CTAG 7 cut(s) 21, 77, 726, 894, 998, 1037, 1167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.