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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 267 |
| AccIII | TCCGGA | 1 cut(s) | 215 |
| AciI | CCGC | 2 cut(s) | 103, 267 |
| AclWI | GGATC | 2 cut(s) | 113, 137 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 222, 424 |
| AluBI | AGCT | 3 cut(s) | 198, 213, 415 |
| AluI | AGCT | 3 cut(s) | 198, 213, 415 |
| AlwI | GGATC | 2 cut(s) | 113, 137 |
| Aor13HI | TCCGGA | 1 cut(s) | 215 |
| AoxI | GGCC | 1 cut(s) | 86 |
| AsuC2I | CCSGG | 1 cut(s) | 147 |
| AsuHPI | GGTGA | 1 cut(s) | 161 |
| AsuNHI | GCTAGC | 1 cut(s) | 209 |
| BbsI | GAAGAC | 1 cut(s) | 159 |
| BccI | CCATC | 2 cut(s) | 318, 345 |
| BceAI | ACGGC | 1 cut(s) | 101 |
| BciVI | GTATCC | 1 cut(s) | 105 |
| BclI | TGATCA | 1 cut(s) | 234 |
| BcnI | CCSGG | 1 cut(s) | 147 |
| BfaI | CTAG | 1 cut(s) | 210 |
| BfuI | GTATCC | 1 cut(s) | 105 |
| Bme1390I | CCNGG | 1 cut(s) | 147 |
| BmrFI | CCNGG | 1 cut(s) | 147 |
| BmtI | GCTAGC | 1 cut(s) | 213 |
| BpiI | GAAGAC | 1 cut(s) | 159 |
| BpuMI | CCSGG | 1 cut(s) | 147 |
| BsaJI | CCNNGG | 2 cut(s) | 98, 178 |
| BsaWI | WCCGGW | 1 cut(s) | 215 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 399, 429 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 222, 424 |
| Bse1I | ACTGG | 3 cut(s) | 132, 227, 394 |
| BseAI | TCCGGA | 1 cut(s) | 215 |
| BseDI | CCNNGG | 2 cut(s) | 98, 178 |
| BseGI | GGATG | 2 cut(s) | 329, 337 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 222, 424 |
| BseNI | ACTGG | 3 cut(s) | 132, 227, 394 |
| BseRI | GAGGAG | 1 cut(s) | 167 |
| Bsh1236I | CGCG | 1 cut(s) | 267 |
| BshFI | GGCC | 1 cut(s) | 88 |
| BsiSI | CCGG | 2 cut(s) | 147, 216 |
| BslFI | GGGAC | 2 cut(s) | 181, 420 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 222, 424 |
| BsmFI | GGGAC | 2 cut(s) | 181, 420 |
| BsnI | GGCC | 1 cut(s) | 88 |
| Bsp13I | TCCGGA | 1 cut(s) | 215 |
| Bsp143I | GATC | 3 cut(s) | 105, 142, 234 |
| Bsp19I | CCATGG | 1 cut(s) | 98 |
| BspACI | CCGC | 2 cut(s) | 103, 267 |
| BspANI | GGCC | 1 cut(s) | 88 |
| BspEI | TCCGGA | 1 cut(s) | 215 |
| BspFNI | CGCG | 1 cut(s) | 267 |
| BspOI | GCTAGC | 1 cut(s) | 213 |
| BspPI | GGATC | 2 cut(s) | 113, 137 |
| BsrI | ACTGG | 3 cut(s) | 132, 227, 394 |
| BssECI | CCNNGG | 2 cut(s) | 98, 178 |
| BssMI | GATC | 3 cut(s) | 105, 142, 234 |
| BssT1I | CCWWGG | 1 cut(s) | 98 |
| Bst6I | CTCTTC | 1 cut(s) | 15 |
| BstC8I | GCNNGC | 1 cut(s) | 211 |
| BstDSI | CCRYGG | 1 cut(s) | 98 |
| BstF5I | GGATG | 2 cut(s) | 329, 337 |
| BstFNI | CGCG | 1 cut(s) | 267 |
| BstKTI | GATC | 3 cut(s) | 108, 145, 237 |
| BstMBI | GATC | 3 cut(s) | 105, 142, 234 |
| BstNSI | RCATGY | 1 cut(s) | 78 |
| BstSCI | CCNGG | 1 cut(s) | 145 |
| BstUI | CGCG | 1 cut(s) | 267 |
| BstV2I | GAAGAC | 1 cut(s) | 159 |
| BsuI | GTATCC | 1 cut(s) | 105 |
| BsuRI | GGCC | 1 cut(s) | 88 |
| BtgI | CCRYGG | 1 cut(s) | 98 |
| BtsCI | GGATG | 2 cut(s) | 329, 337 |
| Cac8I | GCNNGC | 1 cut(s) | 211 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 299, 334 |
| CviAII | CATG | 2 cut(s) | 75, 99 |
| CviJI | RGCY | 7 cut(s) | 88, 198, 209, 213, 226, 289, 415 |
| CviKI_1 | RGCY | 7 cut(s) | 88, 198, 209, 213, 226, 289, 415 |
| DpnI | GATC | 3 cut(s) | 107, 144, 236 |
| DpnII | GATC | 3 cut(s) | 105, 142, 234 |
| Eam1104I | CTCTTC | 1 cut(s) | 15 |
| EarI | CTCTTC | 1 cut(s) | 15 |
| EciI | GGCGGA | 1 cut(s) | 118 |
| Eco130I | CCWWGG | 1 cut(s) | 98 |
| EcoT14I | CCWWGG | 1 cut(s) | 98 |
| ErhI | CCWWGG | 1 cut(s) | 98 |
| FaeI | CATG | 2 cut(s) | 78, 102 |
| FaqI | GGGAC | 2 cut(s) | 181, 420 |
| FatI | CATG | 2 cut(s) | 74, 98 |
| FbaI | TGATCA | 1 cut(s) | 234 |
| FokI | GGATG | 2 cut(s) | 324, 336 |
| FspBI | CTAG | 1 cut(s) | 210 |
| HaeIII | GGCC | 1 cut(s) | 88 |
| HapII | CCGG | 2 cut(s) | 147, 216 |
| Hin1II | CATG | 2 cut(s) | 78, 102 |
| HinfI | GANTC | 5 cut(s) | 125, 183, 254, 282, 376 |
| HpaII | CCGG | 2 cut(s) | 147, 216 |
| HphI | GGTGA | 1 cut(s) | 161 |
| Hpy166II | GTNNAC | 1 cut(s) | 356 |
| Hpy188I | TCNGA | 3 cut(s) | 182, 190, 419 |
| Hpy188III | TCNNGA | 3 cut(s) | 50, 216, 229 |
| Hpy8I | GTNNAC | 1 cut(s) | 356 |
| Hsp92II | CATG | 2 cut(s) | 78, 102 |
| Kpn2I | TCCGGA | 1 cut(s) | 215 |
| Ksp22I | TGATCA | 1 cut(s) | 234 |
| Kzo9I | GATC | 3 cut(s) | 105, 142, 234 |
| LmnI | GCTCC | 2 cut(s) | 218, 420 |
| LpnPI | CCDG | 5 cut(s) | 145, 160, 208, 229, 375 |
| MaeI | CTAG | 1 cut(s) | 210 |
| MalI | GATC | 3 cut(s) | 107, 144, 236 |
| MboI | GATC | 3 cut(s) | 105, 142, 234 |
| MboII | GAAGA | 4 cut(s) | 32, 120, 164, 233 |
| MlyI | GAGTC | 2 cut(s) | 177, 263 |
| MnlI | CCTC | 3 cut(s) | 99, 188, 344 |
| MroI | TCCGGA | 1 cut(s) | 215 |
| MspI | CCGG | 2 cut(s) | 147, 216 |
| MspR9I | CCNGG | 1 cut(s) | 147 |
| MvnI | CGCG | 1 cut(s) | 267 |
| NciI | CCSGG | 1 cut(s) | 147 |
| NcoI | CCATGG | 1 cut(s) | 98 |
| NdeII | GATC | 3 cut(s) | 105, 142, 234 |
| NheI | GCTAGC | 1 cut(s) | 209 |
| NlaIII | CATG | 2 cut(s) | 78, 102 |
| NspI | RCATGY | 1 cut(s) | 78 |
| PfeI | GAWTC | 3 cut(s) | 125, 282, 376 |
| PleI | GAGTC | 2 cut(s) | 177, 262 |
| PpsI | GAGTC | 2 cut(s) | 177, 262 |
| Sau3AI | GATC | 3 cut(s) | 105, 142, 234 |
| SchI | GAGTC | 2 cut(s) | 177, 263 |
| ScrFI | CCNGG | 1 cut(s) | 147 |
| SetI | ASST | 3 cut(s) | 200, 215, 417 |
| SsiI | CCGC | 2 cut(s) | 103, 267 |
| SspMI | CTAG | 1 cut(s) | 210 |
| StyD4I | CCNGG | 1 cut(s) | 145 |
| StyI | CCWWGG | 1 cut(s) | 98 |
| TaqI | TCGA | 2 cut(s) | 11, 285 |
| TfiI | GAWTC | 3 cut(s) | 125, 282, 376 |
| TspGWI | ACGGA | 1 cut(s) | 234 |
| XceI | RCATGY | 1 cut(s) | 78 |
| XspI | CTAG | 1 cut(s) | 210 |