AT3G23730

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0000406
Physical Location & Seq
Forward (+)
8550037 .. 8551403
1367 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G23730.1

Sequence Viewer

Length: 876 bp
ATGGGTCGAATCTTGAACCGTACCGTGTTAATGACTCTTCTAGTCGTAACAATGGCCGGAACAGCATTCTCCGGTAGCTTCAACGAAGAGTTTGACTTAACTTGGGGTGAACACAGAGGCAAAATCTTCAGTGGAGGAAAAATGTTGTCACTCTCACTAGACCGGGTTTCCGGGTCGGGTTTTAAATCCAAGAAAGAATATTTGTTCGGAAGAATCGACATGCAGCTTAAACTCGTCGCCGGTAACTCCGCTGGAACCGTCACTGCCTACTACTTGTCATCGGAAGGACCAACACACGACGAGATAGACTTTGAGTTTCTTGGTAATGAAACAGGGAAGCCTTATGTTCTTCACACTAATGTATTTGCTCAAGGCAAAGGAAACAGAGAACAACAGTTTTATCTCTGGTTTGATCCAACCAAGAACTTCCACACTTATTCTCTTGTCTGGAGACCACAACACATCATATTTATGGTAGATAATGTTCCAATCAGAGTATTCAACAATGCAGAGCAACTTGGTGTTCCATTTCCCAAGAACCAACCAATGAAGATATACTCGAGTTTATGGAATGCAGATGATTGGGCTACAAGAGGTGGTTTGGTTAAGACAGATTGGTCTAAAGCTCCTTTCACAGCTTACTACAGAGGCTTTAACGCTGCAGCTTGTACTGTTTCTTCAGGGTCATCTTTCTGTGATCCTAAGTTTAAGAGTTCTTTTACTAATGGTGAATCTCAAGTGGCTAATGAGCTTAATGCTTATGGGAGAAGAAGATTAAGATGGGTTCAGAAGTATTTTATGATTTATGATTATTGTTCTGATTTAAAAAGGTTTCCTCAAGGATTCCCACCAGAGTGTAGGAAGTCTAGAGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_16 XET_C

Protein Analysis

291

Amino Acids

33.15

Weight (kDa)

9.31

Isoelectric Point (pI)

35.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 28 - 208 2.7e-67 Glycosyl hydrolases family 16
XET_C PF06955 246 - 286 4.8e-18 Xyloglucan endo-transglycosylase (XET) C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000406)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23730 AT4G14130
fragaria_vesca FvH4_3g06510 FvH4_4g12960 FvH4_6g38150 FvH4_6g38150 FvH4_6g38150 FvH4_6g38160 FvH4_6g38170 FvH4_6g38181 FvH4_6g38190
malus_domestica MD04G1020100.v1.1 MD09G1152400.v1.1 MD09G1152600.v1.1 MD09G1152700.v1.1 MD17G1139900.v1.1 MD17G1140000.v1.1
prunus_persica Prupe.1G169700_v2.0.a1 Prupe.3G171500_v2.0.a1 Prupe.3G171600_v2.0.a1 Prupe.3G171700_v2.0.a1 Prupe.3G171800_v2.0.a1 Prupe.3G172000_v2.0.a1 Prupe.3G172100_v2.0.a1 Prupe.4G072800_v2.0.a1
pyrus_communis pycom04g01620 pycom09g07160 pycom09g07220 pycom09g07230 pycom10g22480 pycom13g29190 pycom17g13320
rosa_chinensis RchiOBHm_Chr2g0151371 RchiOBHm_Chr2g0151381 RchiOBHm_Chr2g0151401 RchiOBHm_Chr2g0151421 RchiOBHm_Chr2g0151431 RchiOBHm_Chr2g0151441 RchiOBHm_Chr2g0152071 RchiOBHm_Chr4g0412651 RchiOBHm_Chr5g0012671
rosa_laevigata RLG00000008270 RLG00000020544 RLG00000020546 RLG00000020547 RLG00000020548 RLG00000020549 RLG00000020605 RLG00000031947
rosa_multiflora Rmu_co8167812.1_g000001 Rmu_co8363165.1_g000001 Rmu_co8513985.1_g000001 Rmu_sc0000940.1_g000002 Rmu_sc0000940.1_g000005 Rmu_sc0000940.1_g000006 Rmu_sc0000940.1_g000009 Rmu_sc0002516.1_g000015 Rmu_sc0002516.1_g000017 Rmu_sc0002516.1_g000018 Rmu_sc0043125.1_g000001
rosa_roxburghii Rroxscaffold_1G00063930 Rroxscaffold_2G00095990 Rroxscaffold_2G00096470 Rroxscaffold_2G00096480 Rroxscaffold_2G00096490 Rroxscaffold_2G00096500 Rroxscaffold_5G00356830
rosa_rugosa Rorug02G0425600 Rorug02G0425700 Rorug02G0425800 Rorug02G0425900 Rorug02G0430500 Rorug02G0430600 Rorug04G0115400 Rorug05G0003700
rosa_samantha Rh2BG499200 Rh2CG472700 Rh2CG472800 Rh2CG473000 Rh2CG473100 Rh2CG473300 Rh2CG477800 Rh2DG509800 Rh2DG509900 Rh2DG510100 Rh2DG510200 Rh2DG510300 Rh2DG515000 Rh4AG174700 Rh4BG174000 Rh5AG097500
rosa_wichuraiana Rw2G039850 Rw2G039860 Rw2G039870 Rw2G039880 Rw2G039890 Rw2G039900 Rw2G040400 Rw4G014650 Rw5G008540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 616
AciI CCGC 1 cut(s) 249
AclWI GGATC 2 cut(s) 407, 692
AcoI YGGCCR 1 cut(s) 54
AcuI CTGAAG 2 cut(s) 112, 663
AfaI GTAC 2 cut(s) 22, 670
AgsI TTSAA 3 cut(s) 16, 82, 502
AleI CACNNNNGTG 1 cut(s) 853
AluBI AGCT 6 cut(s) 78, 226, 626, 638, 665, 751
AluI AGCT 6 cut(s) 78, 226, 626, 638, 665, 751
Alw26I GTCTC 1 cut(s) 445
AlwI GGATC 2 cut(s) 407, 692
Ama87I CYCGRG 1 cut(s) 559
AoxI GGCC 1 cut(s) 54
ApeKI GCWGC 3 cut(s) 223, 659, 662
AspS9I GGNCC 1 cut(s) 287
AsuC2I CCSGG 2 cut(s) 164, 172
AsuHPI GGTGA 2 cut(s) 119, 740
AvaI CYCGRG 1 cut(s) 559
AvaII GGWCC 1 cut(s) 287
BarI GAAGNNNNNNTAC 2 cut(s) 661, 693
BbvI GCAGC 3 cut(s) 235, 646, 674
BccI CCATC 1 cut(s) 774
BcnI CCSGG 2 cut(s) 164, 172
BcoDI GTCTC 1 cut(s) 445
BfaI CTAG 3 cut(s) 41, 158, 867
BfmI CTRYAG 2 cut(s) 643, 660
BisI GCNGC 3 cut(s) 224, 660, 663
BlsI GCNGC 3 cut(s) 225, 661, 664
Bme1390I CCNGG 2 cut(s) 164, 172
Bme18I GGWCC 1 cut(s) 287
BmeT110I CYCGRG 1 cut(s) 559
BmgT120I GGNCC 1 cut(s) 287
BmiI GGNNCC 1 cut(s) 256
BmrFI CCNGG 2 cut(s) 164, 172
BpmI CTGGAG 1 cut(s) 469
BpuEI CTTGAG 3 cut(s) 354, 720, 822
BpuMI CCSGG 2 cut(s) 164, 172
BsaI GGTCTC 1 cut(s) 445
BsaWI WCCGGW 1 cut(s) 71
Bse118I RCCGGY 1 cut(s) 239
BseXI GCAGC 3 cut(s) 235, 646, 674
BshFI GGCC 1 cut(s) 56
BsiHKCI CYCGRG 1 cut(s) 559
BsiSI CCGG 5 cut(s) 57, 72, 163, 171, 240
BsmAI GTCTC 1 cut(s) 445
BsmI GAATGC 2 cut(s) 65, 577
BsnI GGCC 1 cut(s) 56
Bso31I GGTCTC 1 cut(s) 445
BsoBI CYCGRG 1 cut(s) 559
Bsp143I GATC 2 cut(s) 412, 697
BspACI CCGC 1 cut(s) 249
BspANI GGCC 1 cut(s) 56
BspLI GGNNCC 1 cut(s) 256
BspMAI CTGCAG 1 cut(s) 664
BspPI GGATC 2 cut(s) 407, 692
BspTNI GGTCTC 1 cut(s) 445
BsrFI RCCGGY 1 cut(s) 239
BssAI RCCGGY 1 cut(s) 239
BssMI GATC 2 cut(s) 412, 697
Bst4CI ACNGT 5 cut(s) 20, 25, 259, 396, 673
Bst6I CTCTTC 2 cut(s) 42, 81
BstDEI CTNAG 1 cut(s) 702
BstKTI GATC 2 cut(s) 415, 700
BstMAI GTCTC 1 cut(s) 445
BstMBI GATC 2 cut(s) 412, 697
BstMWI GCNNNNNNNGC 1 cut(s) 62
BstNSI RCATGY 1 cut(s) 223
BstSCI CCNGG 2 cut(s) 162, 170
BstSFI CTRYAG 2 cut(s) 643, 660
BstV1I GCAGC 3 cut(s) 235, 646, 674
BsuRI GGCC 1 cut(s) 56
BtsI GCAGTG 1 cut(s) 261
BtsIMutI CAGTG 2 cut(s) 136, 261
Cfr10I RCCGGY 1 cut(s) 239
Cfr13I GGNCC 1 cut(s) 287
Csp6I GTAC 2 cut(s) 21, 669
CviAII CATG 1 cut(s) 220
CviQI GTAC 2 cut(s) 21, 669
DdeI CTNAG 1 cut(s) 702
DpnI GATC 2 cut(s) 414, 699
DpnII GATC 2 cut(s) 412, 697
DraI TTTAAA 2 cut(s) 184, 825
DrdI GACNNNNNNGTC 1 cut(s) 616
DseDI GACNNNNNNGTC 1 cut(s) 616
EaeI YGGCCR 1 cut(s) 54
Eam1104I CTCTTC 2 cut(s) 42, 81
EarI CTCTTC 2 cut(s) 42, 81
Eco31I GGTCTC 1 cut(s) 445
Eco47I GGWCC 1 cut(s) 287
Eco57I CTGAAG 2 cut(s) 112, 663
Eco88I CYCGRG 1 cut(s) 559
FaeI CATG 1 cut(s) 223
FaiI YATR 9 cut(s) 221, 345, 467, 473, 556, 568, 762, 800, 807
FatI CATG 1 cut(s) 219
Fnu4HI GCNGC 3 cut(s) 224, 660, 663
Fsp4HI GCNGC 3 cut(s) 224, 660, 663
FspBI CTAG 3 cut(s) 41, 158, 867
GluI GCNGC 3 cut(s) 224, 660, 663
GsuI CTGGAG 1 cut(s) 469
HaeIII GGCC 1 cut(s) 56
HapII CCGG 5 cut(s) 57, 72, 163, 171, 240
Hin1II CATG 1 cut(s) 223
HinfI GANTC 6 cut(s) 9, 34, 213, 731, 843, 870
HpaII CCGG 5 cut(s) 57, 72, 163, 171, 240
HphI GGTGA 2 cut(s) 119, 740
Hpy166II GTNNAC 1 cut(s) 110
Hpy188I TCNGA 5 cut(s) 209, 283, 494, 789, 820
Hpy188III TCNNGA 3 cut(s) 13, 448, 867
Hpy8I GTNNAC 1 cut(s) 110
Hpy99I CGWCG 2 cut(s) 239, 302
HpyAV CCTTC 1 cut(s) 278
HpyCH4III ACNGT 5 cut(s) 20, 25, 259, 396, 673
HpyCH4V TGCA 4 cut(s) 223, 509, 575, 662
HpyF10VI GCNNNNNNNGC 1 cut(s) 62
HpyF3I CTNAG 1 cut(s) 702
Hsp92II CATG 1 cut(s) 223
Kzo9I GATC 2 cut(s) 412, 697
LmnI GCTCC 1 cut(s) 631
Lsp1109I GCAGC 3 cut(s) 235, 646, 674
MaeI CTAG 3 cut(s) 41, 158, 867
MaeIII GTNAC 4 cut(s) 46, 147, 242, 259
MalI GATC 2 cut(s) 414, 699
MboI GATC 2 cut(s) 412, 697
MboII GAAGA 9 cut(s) 29, 98, 118, 222, 341, 562, 669, 780, 783
MlyI GAGTC 1 cut(s) 28
MmeI TCCRAC 1 cut(s) 440
MnlI CCTC 5 cut(s) 110, 128, 587, 641, 846
MslI CAYNNNNRTG 3 cut(s) 357, 470, 853
MspA1I CMGCKG 1 cut(s) 251
MspI CCGG 5 cut(s) 57, 72, 163, 171, 240
MspR9I CCNGG 2 cut(s) 164, 172
Mva1269I GAATGC 2 cut(s) 65, 577
MwoI GCNNNNNNNGC 1 cut(s) 62
NciI CCSGG 2 cut(s) 164, 172
NdeII GATC 2 cut(s) 412, 697
NlaIII CATG 1 cut(s) 223
NlaIV GGNNCC 1 cut(s) 256
NmuCI GTSAC 2 cut(s) 147, 259
NspI RCATGY 1 cut(s) 223
OliI CACNNNNGTG 1 cut(s) 853
PaeR7I CTCGAG 1 cut(s) 559
PcsI WCGNNNNNNNCGW 1 cut(s) 213
PctI GAATGC 2 cut(s) 65, 577
PfeI GAWTC 4 cut(s) 9, 213, 731, 843
PkrI GCNGC 3 cut(s) 225, 661, 664
PleI GAGTC 1 cut(s) 28
PpsI GAGTC 1 cut(s) 28
PspN4I GGNNCC 1 cut(s) 256
PspPI GGNCC 1 cut(s) 287
PspXI VCTCGAGB 1 cut(s) 559
PsrI GAACNNNNNNTAC 2 cut(s) 468, 500
PstI CTGCAG 1 cut(s) 664
RsaI GTAC 2 cut(s) 22, 670
RsaNI GTAC 2 cut(s) 21, 669
RseI CAYNNNNRTG 3 cut(s) 357, 470, 853
SatI GCNGC 3 cut(s) 224, 660, 663
Sau3AI GATC 2 cut(s) 412, 697
Sau96I GGNCC 1 cut(s) 287
SchI GAGTC 1 cut(s) 28
ScrFI CCNGG 2 cut(s) 164, 172
SetI ASST 8 cut(s) 80, 228, 598, 628, 640, 667, 753, 833
SfcI CTRYAG 2 cut(s) 643, 660
Sfr274I CTCGAG 1 cut(s) 559
SinI GGWCC 1 cut(s) 287
SlaI CTCGAG 1 cut(s) 559
SmiMI CAYNNNNRTG 3 cut(s) 357, 470, 853
SmlI CTYRAG 4 cut(s) 369, 559, 735, 837
SmoI CTYRAG 4 cut(s) 369, 559, 735, 837
SsiI CCGC 1 cut(s) 249
SspI AATATT 1 cut(s) 200
SspMI CTAG 3 cut(s) 41, 158, 867
StyD4I CCNGG 2 cut(s) 162, 170
TaaI ACNGT 5 cut(s) 20, 25, 259, 396, 673
TaqI TCGA 3 cut(s) 7, 216, 560
TatI WGTACW 1 cut(s) 668
TfiI GAWTC 4 cut(s) 9, 213, 731, 843
TscAI CASTG 2 cut(s) 136, 268
TseFI GTSAC 2 cut(s) 147, 259
TseI GCWGC 3 cut(s) 223, 659, 662
Tsp45I GTSAC 2 cut(s) 147, 259
TspDTI ATGAA 2 cut(s) 342, 563
TspRI CASTG 2 cut(s) 136, 268
VpaK11BI GGWCC 1 cut(s) 287
XbaI TCTAGA 1 cut(s) 866
XceI RCATGY 1 cut(s) 223
XhoI CTCGAG 1 cut(s) 559
XspI CTAG 3 cut(s) 41, 158, 867
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.