Rh4AG174700

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000406
Physical Location & Seq
Forward (+)
43851852 .. 43854747
2896 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG174700.1

Sequence Viewer

Length: 876 bp
ATGGGGATTTCTGGTAACGTGTCTTGTTTGGTTTTGTTGGTTGGTTTAGCATTGAGCTCTTTAATAGTAGGCTCTTATGCTGGCAACTTCTATCAAGACTTTGACTTGACATGGGGTGGTCACCGTCCTAAGATATTCAAGGGAGGTCAGCTTCTTTCTTTGTCTTTGGACAAGGTTTCTGGCTCTGGATTCCAGTCCAAGAAAGAGTACCTGTTCGGGAGGATTGACATGCAACTTAAGCTTGTTGCCGGAAACTCTGCCGGCACTGTGACTGCCTACTACGTACGTATTCGGAGAAATAAAAATATATACTTGTCTTCTCAAGGCCCTACACATGATGAAATCGACTTTGAATTCTTGGGAAATGTCAGTGGAGATCCATATGTGTTGCATACCAATATTTTCACTCAGGGCAAGGGAAACAGAGAGCAGCAATTCTATCTCTGGTTTGACCCCTCAAAAAACTTCCACACTTACTCCATCATCTGGAAGCCCCAGCACATAATTTTCTTGGTGGACAATACTCCCATTAGAGTGTTCAAAAATGCTGAATCACTGGGTGTTCCATTCCCAAAGAACCAAGCCATGAGAATTTACTCGAGCCTTTGGAATGCTGATGATTGGGCTACCAGAGGAGGATTGGTGAAAACTGATTGGTCAAAGGCACCCTTTACAGCATACTACAGAAACTTCAACGTCATCGATGCTAAATCATCCAAATCATTCTCTGATTCTCAGCCTAGTTGGCAGACCAATGCACTTGATGCTCCTAGCCGAAGACGCCTGAGATGGGTTCAGAAGTACTTCATGATCTACAACTACTGCACCGATTTAAAACGCTTCCCACGAGGTTTTCCTGCTGAGTGTAGGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_16 XET_C

Protein Analysis

291

Amino Acids

33.25

Weight (kDa)

9.61

Isoelectric Point (pI)

42.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 31 - 221 4.5e-63 Glycosyl hydrolases family 16
XET_C PF06955 245 - 289 3.8e-18 Xyloglucan endo-transglycosylase (XET) C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000406)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23730 AT4G14130
fragaria_vesca FvH4_3g06510 FvH4_4g12960 FvH4_6g38150 FvH4_6g38150 FvH4_6g38150 FvH4_6g38160 FvH4_6g38170 FvH4_6g38181 FvH4_6g38190
malus_domestica MD04G1020100.v1.1 MD09G1152400.v1.1 MD09G1152600.v1.1 MD09G1152700.v1.1 MD17G1139900.v1.1 MD17G1140000.v1.1
prunus_persica Prupe.1G169700_v2.0.a1 Prupe.3G171500_v2.0.a1 Prupe.3G171600_v2.0.a1 Prupe.3G171700_v2.0.a1 Prupe.3G171800_v2.0.a1 Prupe.3G172000_v2.0.a1 Prupe.3G172100_v2.0.a1 Prupe.4G072800_v2.0.a1
pyrus_communis pycom04g01620 pycom09g07160 pycom09g07220 pycom09g07230 pycom10g22480 pycom13g29190 pycom17g13320
rosa_chinensis RchiOBHm_Chr2g0151371 RchiOBHm_Chr2g0151381 RchiOBHm_Chr2g0151401 RchiOBHm_Chr2g0151421 RchiOBHm_Chr2g0151431 RchiOBHm_Chr2g0151441 RchiOBHm_Chr2g0152071 RchiOBHm_Chr4g0412651 RchiOBHm_Chr5g0012671
rosa_laevigata RLG00000008270 RLG00000020544 RLG00000020546 RLG00000020547 RLG00000020548 RLG00000020549 RLG00000020605 RLG00000031947
rosa_multiflora Rmu_co8167812.1_g000001 Rmu_co8363165.1_g000001 Rmu_co8513985.1_g000001 Rmu_sc0000940.1_g000002 Rmu_sc0000940.1_g000005 Rmu_sc0000940.1_g000006 Rmu_sc0000940.1_g000009 Rmu_sc0002516.1_g000015 Rmu_sc0002516.1_g000017 Rmu_sc0002516.1_g000018 Rmu_sc0043125.1_g000001
rosa_roxburghii Rroxscaffold_1G00063930 Rroxscaffold_2G00095990 Rroxscaffold_2G00096470 Rroxscaffold_2G00096480 Rroxscaffold_2G00096490 Rroxscaffold_2G00096500 Rroxscaffold_5G00356830
rosa_rugosa Rorug02G0425600 Rorug02G0425700 Rorug02G0425800 Rorug02G0425900 Rorug02G0430500 Rorug02G0430600 Rorug04G0115400 Rorug05G0003700
rosa_samantha Rh2BG499200 Rh2CG472700 Rh2CG472800 Rh2CG473000 Rh2CG473100 Rh2CG473300 Rh2CG477800 Rh2DG509800 Rh2DG509900 Rh2DG510100 Rh2DG510200 Rh2DG510300 Rh2DG515000 Rh4AG174700 Rh4BG174000 Rh5AG097500
rosa_wichuraiana Rw2G039850 Rw2G039860 Rw2G039870 Rw2G039880 Rw2G039890 Rw2G039900 Rw2G040400 Rw4G014650 Rw5G008540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 664
AccB7I CCANNNNNTGG 1 cut(s) 486
AclWI GGATC 1 cut(s) 371
AcsI RAATTY 2 cut(s) 353, 591
AcyI GRCGYC 1 cut(s) 781
AdeI CACNNNGTG 1 cut(s) 560
AfaI GTAC 3 cut(s) 209, 285, 803
AfiI CCNNNNNNNGG 2 cut(s) 486, 790
AflII CTTAAG 1 cut(s) 236
AflIII ACRYGT 1 cut(s) 18
AgsI TTSAA 4 cut(s) 139, 353, 541, 694
AluBI AGCT 3 cut(s) 57, 151, 241
AluI AGCT 3 cut(s) 57, 151, 241
Alw21I GWGCWC 1 cut(s) 59
AlwI GGATC 1 cut(s) 371
Ama87I CYCGRG 1 cut(s) 598
AoxI GGCC 1 cut(s) 325
ApeKI GCWGC 1 cut(s) 430
ApoI RAATTY 2 cut(s) 353, 591
Asp700I GAANNNNTTC 1 cut(s) 803
AspS9I GGNCC 1 cut(s) 326
AsuHPI GGTGA 2 cut(s) 113, 655
AvaI CYCGRG 1 cut(s) 598
BanI GGYRCC 1 cut(s) 664
BanII GRGCYC 1 cut(s) 59
BauI CACGAG 1 cut(s) 846
BbsI GAAGAC 2 cut(s) 309, 784
Bbv12I GWGCWC 1 cut(s) 59
BbvI GCAGC 1 cut(s) 442
BccI CCATC 2 cut(s) 488, 783
BfaI CTAG 2 cut(s) 741, 771
BfmI CTRYAG 1 cut(s) 682
BfrI CTTAAG 1 cut(s) 236
BglI GCCNNNNNGGC 1 cut(s) 745
BisI GCNGC 1 cut(s) 431
BlsI GCNGC 1 cut(s) 432
BmcAI AGTACT 1 cut(s) 803
BmeT110I CYCGRG 1 cut(s) 598
BmgT120I GGNCC 1 cut(s) 326
BmiI GGNNCC 1 cut(s) 666
BmrI ACTGGG 1 cut(s) 566
BmsI GCATC 2 cut(s) 694, 754
BmuI ACTGGG 1 cut(s) 566
BpiI GAAGAC 2 cut(s) 309, 784
BpuEI CTTGAG 1 cut(s) 306
Bsa29I ATCGAT 1 cut(s) 702
BsaAI YACGTR 2 cut(s) 283, 287
BsaHI GRCGYC 1 cut(s) 781
BsaXI ACNNNNNCTCC 4 cut(s) 461, 491, 627, 657
Bsc4I CCNNNNNNNGG 2 cut(s) 486, 790
Bse118I RCCGGY 1 cut(s) 260
Bse1I ACTGG 2 cut(s) 193, 561
BseCI ATCGAT 1 cut(s) 702
BseGI GGATG 1 cut(s) 713
BseLI CCNNNNNNNGG 2 cut(s) 486, 790
BseMII CTCAG 4 cut(s) 422, 749, 776, 852
BseNI ACTGG 2 cut(s) 193, 561
BseRI GAGGAG 1 cut(s) 648
BseXI GCAGC 1 cut(s) 442
BseYI CCCAGC 1 cut(s) 495
BsgI GTGCAG 1 cut(s) 808
BshFI GGCC 1 cut(s) 327
BshNI GGYRCC 1 cut(s) 664
BshVI ATCGAT 1 cut(s) 702
BsiHKAI GWGCWC 1 cut(s) 59
BsiHKCI CYCGRG 1 cut(s) 598
BsiSI CCGG 2 cut(s) 249, 261
BsiWI CGTACG 1 cut(s) 283
BslI CCNNNNNNNGG 2 cut(s) 486, 790
BsmI GAATGC 1 cut(s) 616
BsnI GGCC 1 cut(s) 327
BsoBI CYCGRG 1 cut(s) 598
Bsp1286I GDGCHC 1 cut(s) 59
Bsp143I GATC 2 cut(s) 376, 810
BspANI GGCC 1 cut(s) 327
BspCNI CTCAG 4 cut(s) 421, 748, 777, 853
BspDI ATCGAT 1 cut(s) 702
BspHI TCATGA 1 cut(s) 807
BspLI GGNNCC 1 cut(s) 666
BspPI GGATC 1 cut(s) 371
BspT107I GGYRCC 1 cut(s) 664
BspTI CTTAAG 1 cut(s) 236
BsrFI RCCGGY 1 cut(s) 260
BsrI ACTGG 2 cut(s) 193, 561
BssAI RCCGGY 1 cut(s) 260
BssMI GATC 2 cut(s) 376, 810
BssNI GRCGYC 1 cut(s) 781
BssSI CACGAG 1 cut(s) 846
Bst2BI CACGAG 1 cut(s) 846
Bst4CI ACNGT 2 cut(s) 125, 268
BstACI GRCGYC 1 cut(s) 781
BstAFI CTTAAG 1 cut(s) 236
BstAPI GCANNNNNTGC 1 cut(s) 764
BstBAI YACGTR 2 cut(s) 283, 287
BstC8I GCNNGC 2 cut(s) 82, 262
BstDEI CTNAG 5 cut(s) 129, 408, 735, 785, 861
BstEII GGTNACC 1 cut(s) 119
BstF5I GGATG 1 cut(s) 713
BstKTI GATC 2 cut(s) 379, 813
BstMBI GATC 2 cut(s) 376, 810
BstMWI GCNNNNNNNGC 4 cut(s) 238, 745, 764, 780
BstNSI RCATGY 1 cut(s) 232
BstPI GGTNACC 1 cut(s) 119
BstSFI CTRYAG 1 cut(s) 682
BstSNI TACGTA 2 cut(s) 283, 287
BstV1I GCAGC 1 cut(s) 442
BstV2I GAAGAC 2 cut(s) 309, 784
BstX2I RGATCY 1 cut(s) 376
BstYI RGATCY 1 cut(s) 376
Bsu15I ATCGAT 1 cut(s) 702
BsuRI GGCC 1 cut(s) 327
BsuTUI ATCGAT 1 cut(s) 702
BtsCI GGATG 1 cut(s) 713
BtsIMutI CAGTG 3 cut(s) 264, 376, 554
Cac8I GCNNGC 2 cut(s) 82, 262
CciI TCATGA 1 cut(s) 807
Cfr10I RCCGGY 1 cut(s) 260
Cfr13I GGNCC 1 cut(s) 326
ClaI ATCGAT 1 cut(s) 702
CseI GACGC 1 cut(s) 789
Csp6I GTAC 3 cut(s) 208, 284, 802
CviAII CATG 5 cut(s) 111, 229, 335, 586, 808
CviQI GTAC 3 cut(s) 208, 284, 802
DdeI CTNAG 5 cut(s) 129, 408, 735, 785, 861
DpnI GATC 2 cut(s) 378, 812
DpnII GATC 2 cut(s) 376, 810
DraI TTTAAA 1 cut(s) 834
DraIII CACNNNGTG 1 cut(s) 560
Ecl136II GAGCTC 1 cut(s) 57
Eco105I TACGTA 2 cut(s) 283, 287
Eco24I GRGCYC 1 cut(s) 59
Eco53kI GAGCTC 1 cut(s) 57
Eco88I CYCGRG 1 cut(s) 598
Eco91I GGTNACC 1 cut(s) 119
EcoICRI GAGCTC 1 cut(s) 57
EcoO109I RGGNCCY 1 cut(s) 326
EcoO65I GGTNACC 1 cut(s) 119
EcoRI GAATTC 1 cut(s) 353
EcoT38I GRGCYC 1 cut(s) 59
FaeI CATG 5 cut(s) 114, 232, 338, 589, 811
FalI AAGNNNNNCTT 2 cut(s) 653, 685
FatI CATG 5 cut(s) 110, 228, 334, 585, 807
FauNDI CATATG 1 cut(s) 382
Fnu4HI GCNGC 1 cut(s) 431
FokI GGATG 1 cut(s) 700
FriOI GRGCYC 1 cut(s) 59
Fsp4HI GCNGC 1 cut(s) 431
FspBI CTAG 2 cut(s) 741, 771
GluI GCNGC 1 cut(s) 431
GsaI CCCAGC 1 cut(s) 499
HaeIII GGCC 1 cut(s) 327
HapII CCGG 2 cut(s) 249, 261
HgaI GACGC 1 cut(s) 789
Hin1I GRCGYC 1 cut(s) 781
Hin1II CATG 5 cut(s) 114, 232, 338, 589, 811
HindIII AAGCTT 1 cut(s) 239
HinfI GANTC 3 cut(s) 189, 551, 731
HpaII CCGG 2 cut(s) 249, 261
HphI GGTGA 2 cut(s) 113, 655
Hpy166II GTNNAC 1 cut(s) 517
Hpy188I TCNGA 3 cut(s) 294, 730, 798
Hpy188III TCNNGA 5 cut(s) 95, 186, 217, 487, 808
Hpy8I GTNNAC 1 cut(s) 517
HpyCH4III ACNGT 2 cut(s) 125, 268
HpyCH4IV ACGT 4 cut(s) 18, 282, 286, 696
HpyCH4V TGCA 4 cut(s) 232, 391, 758, 825
HpyF10VI GCNNNNNNNGC 4 cut(s) 238, 745, 764, 780
HpyF3I CTNAG 5 cut(s) 129, 408, 735, 785, 861
HpySE526I ACGT 4 cut(s) 18, 282, 286, 696
Hsp92I GRCGYC 1 cut(s) 781
Hsp92II CATG 5 cut(s) 114, 232, 338, 589, 811
KroI GCCGGC 1 cut(s) 260
KroNI GCCGGC 1 cut(s) 262
Kzo9I GATC 2 cut(s) 376, 810
LmnI GCTCC 1 cut(s) 772
Lsp1109I GCAGC 1 cut(s) 442
LweI GCATC 2 cut(s) 694, 754
MaeI CTAG 2 cut(s) 741, 771
MaeII ACGT 4 cut(s) 18, 282, 286, 696
MaeIII GTNAC 3 cut(s) 14, 119, 268
MalI GATC 2 cut(s) 378, 812
MboI GATC 2 cut(s) 376, 810
MboII GAAGA 2 cut(s) 309, 789
MflI RGATCY 1 cut(s) 376
MhlI GDGCHC 1 cut(s) 59
MluCI AATT 4 cut(s) 353, 434, 504, 591
MnlI CCTC 6 cut(s) 137, 213, 466, 626, 629, 842
MroNI GCCGGC 1 cut(s) 260
MroXI GAANNNNTTC 1 cut(s) 803
MseI TTAA 3 cut(s) 62, 237, 833
MslI CAYNNNNRTG 1 cut(s) 533
MspCI CTTAAG 1 cut(s) 236
MspI CCGG 2 cut(s) 249, 261
Mva1269I GAATGC 1 cut(s) 616
MwoI GCNNNNNNNGC 4 cut(s) 238, 745, 764, 780
NaeI GCCGGC 1 cut(s) 262
NdeI CATATG 1 cut(s) 382
NdeII GATC 2 cut(s) 376, 810
NgoMIV GCCGGC 1 cut(s) 260
NlaIII CATG 5 cut(s) 114, 232, 338, 589, 811
NlaIV GGNNCC 1 cut(s) 666
NmuCI GTSAC 2 cut(s) 119, 268
NspI RCATGY 1 cut(s) 232
PaeR7I CTCGAG 1 cut(s) 598
PagI TCATGA 1 cut(s) 807
PcsI WCGNNNNNNNCGW 1 cut(s) 844
PctI GAATGC 1 cut(s) 616
PdiI GCCGGC 1 cut(s) 262
PdmI GAANNNNTTC 1 cut(s) 803
PfeI GAWTC 3 cut(s) 189, 551, 731
Pfl23II CGTACG 1 cut(s) 283
PflMI CCANNNNNTGG 1 cut(s) 486
PkrI GCNGC 1 cut(s) 432
Ppu21I YACGTR 2 cut(s) 283, 287
Psp124BI GAGCTC 1 cut(s) 59
PspEI GGTNACC 1 cut(s) 119
PspFI CCCAGC 1 cut(s) 495
PspLI CGTACG 1 cut(s) 283
PspN4I GGNNCC 1 cut(s) 666
PspPI GGNCC 1 cut(s) 326
PspXI VCTCGAGB 1 cut(s) 598
PsuI RGATCY 1 cut(s) 376
RsaI GTAC 3 cut(s) 209, 285, 803
RsaNI GTAC 3 cut(s) 208, 284, 802
RseI CAYNNNNRTG 1 cut(s) 533
SacI GAGCTC 1 cut(s) 59
SaqAI TTAA 3 cut(s) 62, 237, 833
SatI GCNGC 1 cut(s) 431
Sau3AI GATC 2 cut(s) 376, 810
Sau96I GGNCC 1 cut(s) 326
ScaI AGTACT 1 cut(s) 803
SduI GDGCHC 1 cut(s) 59
SfaNI GCATC 2 cut(s) 694, 754
SfcI CTRYAG 1 cut(s) 682
Sfr274I CTCGAG 1 cut(s) 598
SlaI CTCGAG 1 cut(s) 598
SmiMI CAYNNNNRTG 1 cut(s) 533
SmlI CTYRAG 3 cut(s) 236, 321, 598
SmoI CTYRAG 3 cut(s) 236, 321, 598
SnaBI TACGTA 2 cut(s) 283, 287
Sse9I AATT 4 cut(s) 353, 434, 504, 591
SspI AATATT 1 cut(s) 400
SspMI CTAG 2 cut(s) 741, 771
SstI GAGCTC 1 cut(s) 59
TaaI ACNGT 2 cut(s) 125, 268
TaiI ACGT 4 cut(s) 21, 285, 289, 699
TaqI TCGA 3 cut(s) 345, 599, 702
TasI AATT 4 cut(s) 353, 434, 504, 591
TatI WGTACW 1 cut(s) 801
TfiI GAWTC 3 cut(s) 189, 551, 731
Tru1I TTAA 3 cut(s) 62, 237, 833
Tru9I TTAA 3 cut(s) 62, 237, 833
TscAI CASTG 3 cut(s) 271, 376, 561
TseFI GTSAC 2 cut(s) 119, 268
TseI GCWGC 1 cut(s) 430
Tsp45I GTSAC 2 cut(s) 119, 268
TspDTI ATGAA 2 cut(s) 354, 796
TspRI CASTG 3 cut(s) 271, 376, 561
Van91I CCANNNNNTGG 1 cut(s) 486
Vha464I CTTAAG 1 cut(s) 236
XapI RAATTY 2 cut(s) 353, 591
XceI RCATGY 1 cut(s) 232
XcmI CCANNNNNNNNNTGG 1 cut(s) 637
XhoI CTCGAG 1 cut(s) 598
XmnI GAANNNNTTC 1 cut(s) 803
XspI CTAG 2 cut(s) 741, 771
ZrmI AGTACT 1 cut(s) 803
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.