pycom13g29190

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0000406
Physical Location & Seq
Forward (+)
25684240 .. 25686219
1980 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g29190.3

Sequence Viewer

Length: 828 bp
ATGGGAATTGCTAGTTTGGCTTTGTTAGTGGGATTAGCTTTCAGCTCTTTAATAGCAGCAACTTATGCTGGAAACTTTTATCAAGATTTTGACCTAACATGGGGTGGCAACCGCCCTAAAATATTCAAGGGAGGTCAGCTTCTTTCTCTGTCTTTGGACATAGTTTCTGGCTCCGGGTTTCAGTCCAAGAAAGAGTATCTGTTTGGGAGGATTGATATGCAGCTTAAGCTTGTTCCTGGGAACTCTGCTGGCACCGTCACTGCATACTACATGTCCTCTCAAGGTCCAACACATGATGAAATCGACTTCGAGTTCTTGGGAAATGTTAGCGGCGACCCTTACGTTCTGCACACCAATGTTTTCACTCAGGGCAAGGGAAATAGAGAGCAGCAGTTCTATCTCTGGTTTGACCCCTCAAAAAACTTTCACACTTACTCCATCATCTGGAAGCCCCAGCACATAATCTTCTTGGTTGACAACACTCCCATCAGAGTATTCAAGAACGCTGGAAAAGTTGGTGTCCCATTCCCCAAGAGCCAACCCATGAGGATATACTCAAGCCTTTGGAATGCAGATGACTGGGCCACCAGAGGAGGGTTAGTGAAAACTGATTGGTCAAAGGCACCCTTCACAGCATACTACCGAAACTTCAACGTCATTGATTCCAAATCATCCAATTCCTTATCGGAATGGCAGACCAACGAGCTCGACGCTCCCAGCAGAAGACGTCTGAGATGGGTTCAGAAATATTTCATGATTTACAACTATTGCACCGACTTAAAACGCTTCCCGCGAGGTGCTCCAGCTGAATGCAGACGATCTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_16 XET_C

Protein Analysis

276

Amino Acids

31.34

Weight (kDa)

9.44

Isoelectric Point (pI)

41.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000406)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23730 AT4G14130
fragaria_vesca FvH4_3g06510 FvH4_4g12960 FvH4_6g38150 FvH4_6g38150 FvH4_6g38150 FvH4_6g38160 FvH4_6g38170 FvH4_6g38181 FvH4_6g38190
malus_domestica MD04G1020100.v1.1 MD09G1152400.v1.1 MD09G1152600.v1.1 MD09G1152700.v1.1 MD17G1139900.v1.1 MD17G1140000.v1.1
prunus_persica Prupe.1G169700_v2.0.a1 Prupe.3G171500_v2.0.a1 Prupe.3G171600_v2.0.a1 Prupe.3G171700_v2.0.a1 Prupe.3G171800_v2.0.a1 Prupe.3G172000_v2.0.a1 Prupe.3G172100_v2.0.a1 Prupe.4G072800_v2.0.a1
pyrus_communis pycom04g01620 pycom09g07160 pycom09g07220 pycom09g07230 pycom10g22480 pycom13g29190 pycom17g13320
rosa_chinensis RchiOBHm_Chr2g0151371 RchiOBHm_Chr2g0151381 RchiOBHm_Chr2g0151401 RchiOBHm_Chr2g0151421 RchiOBHm_Chr2g0151431 RchiOBHm_Chr2g0151441 RchiOBHm_Chr2g0152071 RchiOBHm_Chr4g0412651 RchiOBHm_Chr5g0012671
rosa_laevigata RLG00000008270 RLG00000020544 RLG00000020546 RLG00000020547 RLG00000020548 RLG00000020549 RLG00000020605 RLG00000031947
rosa_multiflora Rmu_co8167812.1_g000001 Rmu_co8363165.1_g000001 Rmu_co8513985.1_g000001 Rmu_sc0000940.1_g000002 Rmu_sc0000940.1_g000005 Rmu_sc0000940.1_g000006 Rmu_sc0000940.1_g000009 Rmu_sc0002516.1_g000015 Rmu_sc0002516.1_g000017 Rmu_sc0002516.1_g000018 Rmu_sc0043125.1_g000001
rosa_roxburghii Rroxscaffold_1G00063930 Rroxscaffold_2G00095990 Rroxscaffold_2G00096470 Rroxscaffold_2G00096480 Rroxscaffold_2G00096490 Rroxscaffold_2G00096500 Rroxscaffold_5G00356830
rosa_rugosa Rorug02G0425600 Rorug02G0425700 Rorug02G0425800 Rorug02G0425900 Rorug02G0430500 Rorug02G0430600 Rorug04G0115400 Rorug05G0003700
rosa_samantha Rh2BG499200 Rh2CG472700 Rh2CG472800 Rh2CG473000 Rh2CG473100 Rh2CG473300 Rh2CG477800 Rh2DG509800 Rh2DG509900 Rh2DG510100 Rh2DG510200 Rh2DG510300 Rh2DG515000 Rh4AG174700 Rh4BG174000 Rh5AG097500
rosa_wichuraiana Rw2G039850 Rw2G039860 Rw2G039870 Rw2G039880 Rw2G039890 Rw2G039900 Rw2G040400 Rw4G014650 Rw5G008540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 730
AccB1I GGYRCC 2 cut(s) 251, 622
AccB7I CCANNNNNTGG 1 cut(s) 444
AccII CGCG 1 cut(s) 793
AciI CCGC 3 cut(s) 112, 330, 791
AcyI GRCGYC 1 cut(s) 727
AfiI CCNNNNNNNGG 3 cut(s) 100, 444, 594
AflII CTTAAG 1 cut(s) 224
AflIII ACRYGT 1 cut(s) 270
AgsI TTSAA 3 cut(s) 127, 499, 652
AjnI CCWGG 1 cut(s) 235
AluBI AGCT 7 cut(s) 38, 45, 139, 223, 229, 706, 806
AluI AGCT 7 cut(s) 38, 45, 139, 223, 229, 706, 806
Alw21I GWGCWC 2 cut(s) 708, 802
AoxI GGCC 1 cut(s) 582
ApeKI GCWGC 3 cut(s) 56, 220, 388
Asp700I GAANNNNTTC 1 cut(s) 749
AspS9I GGNCC 2 cut(s) 284, 582
AsuC2I CCSGG 1 cut(s) 175
AvaII GGWCC 1 cut(s) 284
BanI GGYRCC 2 cut(s) 251, 622
BanII GRGCYC 1 cut(s) 708
BbsI GAAGAC 1 cut(s) 730
Bbv12I GWGCWC 2 cut(s) 708, 802
BbvI GCAGC 3 cut(s) 68, 232, 400
BccI CCATC 3 cut(s) 446, 494, 729
BciT130I CCWGG 1 cut(s) 237
BcnI CCSGG 1 cut(s) 175
BfaI CTAG 2 cut(s) 12, 826
BfrI CTTAAG 1 cut(s) 224
BisI GCNGC 4 cut(s) 57, 221, 331, 389
BlsI GCNGC 4 cut(s) 58, 222, 332, 390
Bme1390I CCNGG 2 cut(s) 175, 237
Bme18I GGWCC 1 cut(s) 284
BmgT120I GGNCC 2 cut(s) 284, 582
BmiI GGNNCC 3 cut(s) 172, 253, 624
BmrFI CCNGG 2 cut(s) 175, 237
BmrI ACTGGG 1 cut(s) 589
BmuI ACTGGG 1 cut(s) 589
BpiI GAAGAC 1 cut(s) 730
BpmI CTGGAG 1 cut(s) 786
BpuEI CTTGAG 2 cut(s) 264, 541
BpuMI CCSGG 1 cut(s) 175
BsaHI GRCGYC 1 cut(s) 727
BsaJI CCNNGG 1 cut(s) 236
BsaXI ACNNNNNCTCC 4 cut(s) 419, 449, 585, 615
Bsc4I CCNNNNNNNGG 3 cut(s) 100, 444, 594
Bse1I ACTGG 1 cut(s) 584
BseBI CCWGG 1 cut(s) 237
BseDI CCNNGG 1 cut(s) 236
BseGI GGATG 1 cut(s) 671
BseLI CCNNNNNNNGG 3 cut(s) 100, 444, 594
BseMII CTCAG 2 cut(s) 380, 722
BseNI ACTGG 1 cut(s) 584
BseRI GAGGAG 1 cut(s) 606
BseXI GCAGC 3 cut(s) 68, 232, 400
BseYI CCCAGC 2 cut(s) 453, 716
BsgI GTGCAG 1 cut(s) 332
Bsh1236I CGCG 1 cut(s) 793
BshFI GGCC 1 cut(s) 584
BshNI GGYRCC 2 cut(s) 251, 622
BsiHKAI GWGCWC 2 cut(s) 708, 802
BsiSI CCGG 1 cut(s) 174
BslFI GGGAC 1 cut(s) 506
BslI CCNNNNNNNGG 3 cut(s) 100, 444, 594
BsmFI GGGAC 1 cut(s) 506
BsmI GAATGC 2 cut(s) 574, 815
BsnI GGCC 1 cut(s) 584
Bsp1286I GDGCHC 2 cut(s) 708, 802
Bsp143I GATC 1 cut(s) 818
BspACI CCGC 3 cut(s) 112, 330, 791
BspANI GGCC 1 cut(s) 584
BspCNI CTCAG 2 cut(s) 379, 723
BspFNI CGCG 1 cut(s) 793
BspHI TCATGA 1 cut(s) 753
BspLI GGNNCC 3 cut(s) 172, 253, 624
BspT107I GGYRCC 2 cut(s) 251, 622
BspTI CTTAAG 1 cut(s) 224
BsrI ACTGG 1 cut(s) 584
BssECI CCNNGG 1 cut(s) 236
BssMI GATC 1 cut(s) 818
BssNI GRCGYC 1 cut(s) 727
Bst2UI CCWGG 1 cut(s) 237
Bst4CI ACNGT 1 cut(s) 256
BstACI GRCGYC 1 cut(s) 727
BstAFI CTTAAG 1 cut(s) 224
BstAPI GCANNNNNTGC 1 cut(s) 65
BstC8I GCNNGC 1 cut(s) 250
BstDEI CTNAG 2 cut(s) 366, 731
BstF5I GGATG 1 cut(s) 671
BstFNI CGCG 1 cut(s) 793
BstKTI GATC 1 cut(s) 821
BstMBI GATC 1 cut(s) 818
BstMWI GCNNNNNNNGC 3 cut(s) 17, 65, 226
BstNI CCWGG 1 cut(s) 237
BstNSI RCATGY 1 cut(s) 274
BstSCI CCNGG 2 cut(s) 173, 235
BstUI CGCG 1 cut(s) 793
BstV1I GCAGC 3 cut(s) 68, 232, 400
BstV2I GAAGAC 1 cut(s) 730
BsuRI GGCC 1 cut(s) 584
BtsCI GGATG 1 cut(s) 671
BtsI GCAGTG 1 cut(s) 258
BtsIMutI CAGTG 1 cut(s) 258
Cac8I GCNNGC 1 cut(s) 250
CciI TCATGA 1 cut(s) 753
Cfr13I GGNCC 2 cut(s) 284, 582
CseI GACGC 1 cut(s) 719
CviAII CATG 5 cut(s) 99, 271, 293, 544, 754
DdeI CTNAG 2 cut(s) 366, 731
DpnI GATC 1 cut(s) 820
DpnII GATC 1 cut(s) 818
Ecl136II GAGCTC 1 cut(s) 706
Eco24I GRGCYC 1 cut(s) 708
Eco47I GGWCC 1 cut(s) 284
Eco53kI GAGCTC 1 cut(s) 706
EcoICRI GAGCTC 1 cut(s) 706
EcoRII CCWGG 1 cut(s) 235
EcoT38I GRGCYC 1 cut(s) 708
FaeI CATG 5 cut(s) 102, 274, 296, 547, 757
FalI AAGNNNNNCTT 2 cut(s) 611, 643
FaqI GGGAC 1 cut(s) 506
FatI CATG 5 cut(s) 98, 270, 292, 543, 753
FauI CCCGC 1 cut(s) 798
Fnu4HI GCNGC 4 cut(s) 57, 221, 331, 389
FokI GGATG 1 cut(s) 658
FriOI GRGCYC 1 cut(s) 708
Fsp4HI GCNGC 4 cut(s) 57, 221, 331, 389
FspBI CTAG 2 cut(s) 12, 826
GluI GCNGC 4 cut(s) 57, 221, 331, 389
GsaI CCCAGC 2 cut(s) 457, 720
GsuI CTGGAG 1 cut(s) 786
HaeIII GGCC 1 cut(s) 584
HapII CCGG 1 cut(s) 174
HgaI GACGC 1 cut(s) 719
Hin1I GRCGYC 1 cut(s) 727
Hin1II CATG 5 cut(s) 102, 274, 296, 547, 757
HincII GTYRAC 1 cut(s) 475
HindII GTYRAC 1 cut(s) 475
HindIII AAGCTT 1 cut(s) 227
HinfI GANTC 1 cut(s) 662
HpaII CCGG 1 cut(s) 174
Hpy166II GTNNAC 1 cut(s) 475
Hpy188I TCNGA 4 cut(s) 491, 688, 732, 744
Hpy188III TCNNGA 4 cut(s) 83, 445, 499, 754
Hpy8I GTNNAC 1 cut(s) 475
Hpy99I CGWCG 1 cut(s) 713
HpyAV CCTTC 1 cut(s) 637
HpyCH4III ACNGT 1 cut(s) 256
HpyCH4IV ACGT 3 cut(s) 342, 654, 727
HpyCH4V TGCA 6 cut(s) 220, 263, 349, 572, 771, 813
HpyF10VI GCNNNNNNNGC 3 cut(s) 17, 65, 226
HpyF3I CTNAG 2 cut(s) 366, 731
HpySE526I ACGT 3 cut(s) 342, 654, 727
Hsp92I GRCGYC 1 cut(s) 727
Hsp92II CATG 5 cut(s) 102, 274, 296, 547, 757
Kzo9I GATC 1 cut(s) 818
LmnI GCTCC 3 cut(s) 176, 718, 805
Lsp1109I GCAGC 3 cut(s) 68, 232, 400
MaeI CTAG 2 cut(s) 12, 826
MaeII ACGT 3 cut(s) 342, 654, 727
MaeIII GTNAC 1 cut(s) 256
MalI GATC 1 cut(s) 820
MboI GATC 1 cut(s) 818
MboII GAAGA 2 cut(s) 457, 735
MhlI GDGCHC 2 cut(s) 708, 802
MluCI AATT 2 cut(s) 6, 676
MmeI TCCRAC 1 cut(s) 311
MnlI CCTC 8 cut(s) 125, 201, 286, 424, 540, 584, 587, 788
MroXI GAANNNNTTC 1 cut(s) 749
MseI TTAA 3 cut(s) 50, 225, 779
MslI CAYNNNNRTG 1 cut(s) 354
MspA1I CMGCKG 1 cut(s) 806
MspCI CTTAAG 1 cut(s) 224
MspI CCGG 1 cut(s) 174
MspR9I CCNGG 2 cut(s) 175, 237
Mva1269I GAATGC 2 cut(s) 574, 815
MvaI CCWGG 1 cut(s) 237
MvnI CGCG 1 cut(s) 793
MwoI GCNNNNNNNGC 3 cut(s) 17, 65, 226
NciI CCSGG 1 cut(s) 175
NdeII GATC 1 cut(s) 818
NlaIII CATG 5 cut(s) 102, 274, 296, 547, 757
NlaIV GGNNCC 3 cut(s) 172, 253, 624
NmuCI GTSAC 1 cut(s) 256
NspI RCATGY 1 cut(s) 274
PagI TCATGA 1 cut(s) 753
PciI ACATGT 1 cut(s) 270
PcsI WCGNNNNNNNCGW 1 cut(s) 790
PctI GAATGC 2 cut(s) 574, 815
PdmI GAANNNNTTC 1 cut(s) 749
PfeI GAWTC 1 cut(s) 662
PflMI CCANNNNNTGG 1 cut(s) 444
PkrI GCNGC 4 cut(s) 58, 222, 332, 390
PscI ACATGT 1 cut(s) 270
Psp124BI GAGCTC 1 cut(s) 708
Psp6I CCWGG 1 cut(s) 235
PspFI CCCAGC 2 cut(s) 453, 716
PspGI CCWGG 1 cut(s) 235
PspN4I GGNNCC 3 cut(s) 172, 253, 624
PspPI GGNCC 2 cut(s) 284, 582
PvuII CAGCTG 1 cut(s) 806
RseI CAYNNNNRTG 1 cut(s) 354
SacI GAGCTC 1 cut(s) 708
SaqAI TTAA 3 cut(s) 50, 225, 779
SatI GCNGC 4 cut(s) 57, 221, 331, 389
Sau3AI GATC 1 cut(s) 818
Sau96I GGNCC 2 cut(s) 284, 582
ScrFI CCNGG 2 cut(s) 175, 237
SduI GDGCHC 2 cut(s) 708, 802
SinI GGWCC 1 cut(s) 284
SmiMI CAYNNNNRTG 1 cut(s) 354
SmlI CTYRAG 3 cut(s) 224, 279, 556
SmoI CTYRAG 3 cut(s) 224, 279, 556
Sse9I AATT 2 cut(s) 6, 676
SsiI CCGC 3 cut(s) 112, 330, 791
SspI AATATT 2 cut(s) 123, 749
SspMI CTAG 2 cut(s) 12, 826
SstI GAGCTC 1 cut(s) 708
StyD4I CCNGG 2 cut(s) 173, 235
TaaI ACNGT 1 cut(s) 256
TaiI ACGT 3 cut(s) 345, 657, 730
TaqI TCGA 3 cut(s) 303, 309, 708
TasI AATT 2 cut(s) 6, 676
TauI GCSGC 1 cut(s) 333
TfiI GAWTC 1 cut(s) 662
Tru1I TTAA 3 cut(s) 50, 225, 779
Tru9I TTAA 3 cut(s) 50, 225, 779
TscAI CASTG 1 cut(s) 265
TseFI GTSAC 1 cut(s) 256
TseI GCWGC 3 cut(s) 56, 220, 388
Tsp45I GTSAC 1 cut(s) 256
TspDTI ATGAA 2 cut(s) 312, 742
TspRI CASTG 1 cut(s) 265
Van91I CCANNNNNTGG 1 cut(s) 444
Vha464I CTTAAG 1 cut(s) 224
VpaK11BI GGWCC 1 cut(s) 284
XceI RCATGY 1 cut(s) 274
XmnI GAANNNNTTC 1 cut(s) 749
XspI CTAG 2 cut(s) 12, 826
ZraI GACGTC 1 cut(s) 728
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.