Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 443 |
| AccII | CGCG | 1 cut(s) | 83 |
| AciI | CCGC | 2 cut(s) | 563, 662 |
| AclWI | GGATC | 5 cut(s) | 187, 298, 373, 638, 651 |
| AcuI | CTGAAG | 1 cut(s) | 269 |
| AfaI | GTAC | 2 cut(s) | 166, 778 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 548 |
| AgsI | TTSAA | 4 cut(s) | 52, 150, 468, 621 |
| AjnI | CCWGG | 1 cut(s) | 204 |
| AleI | CACNNNNGTG | 1 cut(s) | 325 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 347, 379 |
| AluBI | AGCT | 3 cut(s) | 37, 198, 592 |
| AluI | AGCT | 3 cut(s) | 37, 198, 592 |
| AlwI | GGATC | 5 cut(s) | 187, 298, 373, 638, 651 |
| Ama87I | CYCGRG | 1 cut(s) | 525 |
| AoxI | GGCC | 3 cut(s) | 389, 623, 813 |
| ApeKI | GCWGC | 1 cut(s) | 730 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 52, 84 |
| AspLEI | GCGC | 1 cut(s) | 85 |
| AspS9I | GGNCC | 1 cut(s) | 253 |
| AvaI | CYCGRG | 1 cut(s) | 525 |
| AvaII | GGWCC | 1 cut(s) | 253 |
| BamHI | GGATCC | 1 cut(s) | 643 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 601, 633 |
| BauI | CACGAG | 1 cut(s) | 199 |
| BbsI | GAAGAC | 1 cut(s) | 580 |
| BbvCI | CCTCAGC | 1 cut(s) | 33 |
| BbvI | GCAGC | 1 cut(s) | 717 |
| BccI | CCATC | 3 cut(s) | 31, 374, 746 |
| BceAI | ACGGC | 1 cut(s) | 404 |
| BciT130I | CCWGG | 1 cut(s) | 206 |
| BfaI | CTAG | 2 cut(s) | 19, 846 |
| BfmI | CTRYAG | 1 cut(s) | 731 |
| BisI | GCNGC | 1 cut(s) | 731 |
| BlsI | GCNGC | 1 cut(s) | 732 |
| BmcAI | AGTACT | 1 cut(s) | 166 |
| Bme1390I | CCNGG | 1 cut(s) | 206 |
| Bme18I | GGWCC | 1 cut(s) | 253 |
| BmeT110I | CYCGRG | 1 cut(s) | 525 |
| BmgT120I | GGNCC | 1 cut(s) | 253 |
| BmiI | GGNNCC | 1 cut(s) | 645 |
| BmrFI | CCNGG | 1 cut(s) | 206 |
| BmrI | ACTGGG | 1 cut(s) | 558 |
| BmsI | GCATC | 2 cut(s) | 688, 717 |
| BmuI | ACTGGG | 1 cut(s) | 558 |
| BpiI | GAAGAC | 1 cut(s) | 580 |
| BpmI | CTGGAG | 1 cut(s) | 320 |
| Bpu10I | CCTNAGC | 1 cut(s) | 33 |
| BsaJI | CCNNGG | 1 cut(s) | 810 |
| BsaWI | WCCGGW | 1 cut(s) | 819 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 576, 606 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 548 |
| Bse1I | ACTGG | 2 cut(s) | 553, 586 |
| BseBI | CCWGG | 1 cut(s) | 206 |
| BseDI | CCNNGG | 1 cut(s) | 810 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 548 |
| BseMII | CTCAG | 1 cut(s) | 47 |
| BseNI | ACTGG | 2 cut(s) | 553, 586 |
| BseRI | GAGGAG | 3 cut(s) | 639, 642, 645 |
| BseXI | GCAGC | 1 cut(s) | 717 |
| Bsh1236I | CGCG | 1 cut(s) | 83 |
| Bsh1285I | CGRYCG | 1 cut(s) | 824 |
| BshFI | GGCC | 3 cut(s) | 391, 625, 815 |
| BsiEI | CGRYCG | 1 cut(s) | 824 |
| BsiHKCI | CYCGRG | 1 cut(s) | 525 |
| BsiSI | CCGG | 1 cut(s) | 820 |
| BslFI | GGGAC | 1 cut(s) | 361 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 548 |
| BsmFI | GGGAC | 1 cut(s) | 361 |
| BsmI | GAATGC | 2 cut(s) | 543, 832 |
| BsnI | GGCC | 3 cut(s) | 391, 625, 815 |
| BsoBI | CYCGRG | 1 cut(s) | 525 |
| Bsp1407I | TGTACA | 1 cut(s) | 776 |
| Bsp143I | GATC | 6 cut(s) | 75, 179, 303, 378, 643, 835 |
| BspACI | CCGC | 2 cut(s) | 563, 662 |
| BspANI | GGCC | 3 cut(s) | 391, 625, 815 |
| BspCNI | CTCAG | 1 cut(s) | 46 |
| BspFNI | CGCG | 1 cut(s) | 83 |
| BspHI | TCATGA | 1 cut(s) | 261 |
| BspLI | GGNNCC | 1 cut(s) | 645 |
| BspMAI | CTGCAG | 1 cut(s) | 735 |
| BspPI | GGATC | 5 cut(s) | 187, 298, 373, 638, 651 |
| BsrGI | TGTACA | 1 cut(s) | 776 |
| BsrI | ACTGG | 2 cut(s) | 553, 586 |
| BssECI | CCNNGG | 1 cut(s) | 810 |
| BssMI | GATC | 6 cut(s) | 75, 179, 303, 378, 643, 835 |
| BssSI | CACGAG | 1 cut(s) | 199 |
| BssT1I | CCWWGG | 1 cut(s) | 810 |
| Bst2BI | CACGAG | 1 cut(s) | 199 |
| Bst2UI | CCWGG | 1 cut(s) | 206 |
| Bst4CI | ACNGT | 3 cut(s) | 107, 225, 675 |
| Bst6I | CTCTTC | 1 cut(s) | 20 |
| BstAUI | TGTACA | 1 cut(s) | 776 |
| BstC8I | GCNNGC | 4 cut(s) | 219, 565, 590, 832 |
| BstDEI | CTNAG | 2 cut(s) | 33, 86 |
| BstFNI | CGCG | 1 cut(s) | 83 |
| BstHHI | GCGC | 1 cut(s) | 85 |
| BstKTI | GATC | 6 cut(s) | 78, 182, 306, 381, 646, 838 |
| BstMBI | GATC | 6 cut(s) | 75, 179, 303, 378, 643, 835 |
| BstMCI | CGRYCG | 1 cut(s) | 824 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 28, 80, 195, 637, 676, 744 |
| BstNI | CCWGG | 1 cut(s) | 206 |
| BstNSI | RCATGY | 1 cut(s) | 189 |
| BstSCI | CCNGG | 1 cut(s) | 204 |
| BstSFI | CTRYAG | 1 cut(s) | 731 |
| BstUI | CGCG | 1 cut(s) | 83 |
| BstV1I | GCAGC | 1 cut(s) | 717 |
| BstV2I | GAAGAC | 1 cut(s) | 580 |
| BstX2I | RGATCY | 2 cut(s) | 303, 643 |
| BstYI | RGATCY | 2 cut(s) | 303, 643 |
| BsuRI | GGCC | 3 cut(s) | 391, 625, 815 |
| BtsI | GCAGTG | 2 cut(s) | 26, 599 |
| BtsIMutI | CAGTG | 3 cut(s) | 26, 221, 599 |
| Cac8I | GCNNGC | 4 cut(s) | 219, 565, 590, 832 |
| CciI | TCATGA | 1 cut(s) | 261 |
| CfoI | GCGC | 1 cut(s) | 85 |
| Cfr13I | GGNCC | 1 cut(s) | 253 |
| CseI | GACGC | 1 cut(s) | 594 |
| Csp6I | GTAC | 2 cut(s) | 165, 777 |
| CviAII | CATG | 5 cut(s) | 186, 262, 474, 707, 771 |
| CviQI | GTAC | 2 cut(s) | 165, 777 |
| DdeI | CTNAG | 2 cut(s) | 33, 86 |
| DpnI | GATC | 6 cut(s) | 77, 181, 305, 380, 645, 837 |
| DpnII | GATC | 6 cut(s) | 75, 179, 303, 378, 643, 835 |
| Eam1104I | CTCTTC | 1 cut(s) | 20 |
| EarI | CTCTTC | 1 cut(s) | 20 |
| Eco130I | CCWWGG | 1 cut(s) | 810 |
| Eco147I | AGGCCT | 1 cut(s) | 815 |
| Eco47I | GGWCC | 1 cut(s) | 253 |
| Eco57I | CTGAAG | 1 cut(s) | 269 |
| Eco88I | CYCGRG | 1 cut(s) | 525 |
| EcoRII | CCWGG | 1 cut(s) | 204 |
| EcoT14I | CCWWGG | 1 cut(s) | 810 |
| ErhI | CCWWGG | 1 cut(s) | 810 |
| FaeI | CATG | 5 cut(s) | 189, 265, 477, 710, 774 |
| FaiI | YATR | 7 cut(s) | 187, 234, 263, 433, 475, 708, 772 |
| FaqI | GGGAC | 1 cut(s) | 361 |
| FatI | CATG | 5 cut(s) | 185, 261, 473, 706, 770 |
| FauI | CCCGC | 1 cut(s) | 556 |
| FblI | GTMKAC | 1 cut(s) | 443 |
| Fnu4HI | GCNGC | 1 cut(s) | 731 |
| Fsp4HI | GCNGC | 1 cut(s) | 731 |
| FspBI | CTAG | 2 cut(s) | 19, 846 |
| GlaI | GCGC | 1 cut(s) | 84 |
| GluI | GCNGC | 1 cut(s) | 731 |
| GsuI | CTGGAG | 1 cut(s) | 320 |
| HaeIII | GGCC | 3 cut(s) | 391, 625, 815 |
| HapII | CCGG | 1 cut(s) | 820 |
| HgaI | GACGC | 1 cut(s) | 594 |
| HhaI | GCGC | 1 cut(s) | 85 |
| Hin1II | CATG | 5 cut(s) | 189, 265, 477, 710, 774 |
| Hin6I | GCGC | 1 cut(s) | 83 |
| HinP1I | GCGC | 1 cut(s) | 83 |
| HincII | GTYRAC | 1 cut(s) | 444 |
| HindII | GTYRAC | 1 cut(s) | 444 |
| HinfI | GANTC | 4 cut(s) | 478, 528, 714, 803 |
| HpaII | CCGG | 1 cut(s) | 820 |
| Hpy166II | GTNNAC | 2 cut(s) | 444, 777 |
| Hpy188I | TCNGA | 4 cut(s) | 175, 249, 460, 792 |
| Hpy188III | TCNNGA | 4 cut(s) | 52, 262, 299, 414 |
| Hpy8I | GTNNAC | 2 cut(s) | 444, 777 |
| Hpy99I | CGWCG | 1 cut(s) | 547 |
| HpyAV | CCTTC | 5 cut(s) | 91, 170, 244, 402, 826 |
| HpyCH4III | ACNGT | 3 cut(s) | 107, 225, 675 |
| HpyCH4V | TGCA | 6 cut(s) | 189, 679, 706, 733, 759, 830 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 28, 80, 195, 637, 676, 744 |
| HpyF3I | CTNAG | 2 cut(s) | 33, 86 |
| Hsp92II | CATG | 5 cut(s) | 189, 265, 477, 710, 774 |
| HspAI | GCGC | 1 cut(s) | 83 |
| Kzo9I | GATC | 6 cut(s) | 75, 179, 303, 378, 643, 835 |
| LmnI | GCTCC | 1 cut(s) | 597 |
| Lsp1109I | GCAGC | 1 cut(s) | 717 |
| LweI | GCATC | 2 cut(s) | 688, 717 |
| MaeI | CTAG | 2 cut(s) | 19, 846 |
| MaeIII | GTNAC | 2 cut(s) | 208, 225 |
| MalI | GATC | 6 cut(s) | 77, 181, 305, 380, 645, 837 |
| MboI | GATC | 6 cut(s) | 75, 179, 303, 378, 643, 835 |
| MboII | GAAGA | 2 cut(s) | 7, 585 |
| MflI | RGATCY | 2 cut(s) | 303, 643 |
| MluCI | AATT | 2 cut(s) | 42, 361 |
| MlyI | GAGTC | 2 cut(s) | 537, 708 |
| MmeI | TCCRAC | 1 cut(s) | 280 |
| MseI | TTAA | 1 cut(s) | 797 |
| MslI | CAYNNNNRTG | 2 cut(s) | 323, 325 |
| MspA1I | CMGCKG | 1 cut(s) | 37 |
| MspI | CCGG | 1 cut(s) | 820 |
| MspR9I | CCNGG | 1 cut(s) | 206 |
| Mva1269I | GAATGC | 2 cut(s) | 543, 832 |
| MvaI | CCWGG | 1 cut(s) | 206 |
| MvnI | CGCG | 1 cut(s) | 83 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 28, 80, 195, 637, 676, 744 |
| NdeII | GATC | 6 cut(s) | 75, 179, 303, 378, 643, 835 |
| NlaIII | CATG | 5 cut(s) | 189, 265, 477, 710, 774 |
| NlaIV | GGNNCC | 1 cut(s) | 645 |
| NmeAIII | GCCGAG | 1 cut(s) | 193 |
| NmuCI | GTSAC | 1 cut(s) | 225 |
| NspI | RCATGY | 1 cut(s) | 189 |
| OliI | CACNNNNGTG | 1 cut(s) | 325 |
| PaeR7I | CTCGAG | 1 cut(s) | 525 |
| PagI | TCATGA | 1 cut(s) | 261 |
| PceI | AGGCCT | 1 cut(s) | 815 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 179 |
| PctI | GAATGC | 2 cut(s) | 543, 832 |
| PfeI | GAWTC | 2 cut(s) | 478, 803 |
| PkrI | GCNGC | 1 cut(s) | 732 |
| PleI | GAGTC | 2 cut(s) | 536, 708 |
| PpsI | GAGTC | 2 cut(s) | 536, 708 |
| Psp6I | CCWGG | 1 cut(s) | 204 |
| PspGI | CCWGG | 1 cut(s) | 204 |
| PspN4I | GGNNCC | 1 cut(s) | 645 |
| PspPI | GGNCC | 1 cut(s) | 253 |
| PspXI | VCTCGAGB | 1 cut(s) | 525 |
| PstI | CTGCAG | 1 cut(s) | 735 |
| PsuI | RGATCY | 2 cut(s) | 303, 643 |
| PvuII | CAGCTG | 1 cut(s) | 37 |
| RsaI | GTAC | 2 cut(s) | 166, 778 |
| RsaNI | GTAC | 2 cut(s) | 165, 777 |
| RseI | CAYNNNNRTG | 2 cut(s) | 323, 325 |
| SalI | GTCGAC | 1 cut(s) | 442 |
| SaqAI | TTAA | 1 cut(s) | 797 |
| SatI | GCNGC | 1 cut(s) | 731 |
| Sau3AI | GATC | 6 cut(s) | 75, 179, 303, 378, 643, 835 |
| Sau96I | GGNCC | 1 cut(s) | 253 |
| ScaI | AGTACT | 1 cut(s) | 166 |
| SchI | GAGTC | 2 cut(s) | 537, 708 |
| ScrFI | CCNGG | 1 cut(s) | 206 |
| SetI | ASST | 9 cut(s) | 13, 39, 125, 200, 255, 403, 594, 797, 844 |
| SfaNI | GCATC | 2 cut(s) | 688, 717 |
| SfcI | CTRYAG | 1 cut(s) | 731 |
| Sfr274I | CTCGAG | 1 cut(s) | 525 |
| SinI | GGWCC | 1 cut(s) | 253 |
| SlaI | CTCGAG | 1 cut(s) | 525 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 323, 325 |
| SmlI | CTYRAG | 1 cut(s) | 525 |
| SmoI | CTYRAG | 1 cut(s) | 525 |
| Sse9I | AATT | 2 cut(s) | 42, 361 |
| SseBI | AGGCCT | 1 cut(s) | 815 |
| SsiI | CCGC | 2 cut(s) | 563, 662 |
| SspMI | CTAG | 2 cut(s) | 19, 846 |
| StuI | AGGCCT | 1 cut(s) | 815 |
| StyD4I | CCNGG | 1 cut(s) | 204 |
| StyI | CCWWGG | 1 cut(s) | 810 |
| TaaI | ACNGT | 3 cut(s) | 107, 225, 675 |
| TasI | AATT | 2 cut(s) | 42, 361 |
| TatI | WGTACW | 2 cut(s) | 164, 776 |
| TfiI | GAWTC | 2 cut(s) | 478, 803 |
| Tru1I | TTAA | 1 cut(s) | 797 |
| Tru9I | TTAA | 1 cut(s) | 797 |
| TscAI | CASTG | 3 cut(s) | 33, 228, 606 |
| TseFI | GTSAC | 1 cut(s) | 225 |
| TseI | GCWGC | 1 cut(s) | 730 |
| Tsp45I | GTSAC | 1 cut(s) | 225 |
| TspDTI | ATGAA | 2 cut(s) | 281, 759 |
| TspRI | CASTG | 3 cut(s) | 33, 228, 606 |
| VpaK11BI | GGWCC | 1 cut(s) | 253 |
| XceI | RCATGY | 1 cut(s) | 189 |
| XhoI | CTCGAG | 1 cut(s) | 525 |
| XmiI | GTMKAC | 1 cut(s) | 443 |
| XspI | CTAG | 2 cut(s) | 19, 846 |
| ZrmI | AGTACT | 1 cut(s) | 166 |