pycom10g22480

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0000406
Physical Location & Seq
Reverse (-)
24837079 .. 24838499
1421 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g22480.2

Sequence Viewer

Length: 858 bp
ATGTTGCTACTCTCCCTCATTCTCTGTTTATTTATGGTTGTGGCATCAGCTGGCAATTTCCAACAAGATTTTGACCTAACTTGGGGTGACCATCGTGCTAAAATAATCGGTGGAGGCAATGTTCTGCAGCTTTCACTTGACCAGGCCTCTGGCTCTGGCTTCCAATCCAAGAAGCAATATCTTTTTGGCAGGATAGAAATGCAGCTCAAGCTTGTTGCAGGAAACTCCGCCGGCACCGTCACTGCCTACTATTTGTCTTCCGAAGGGCCAAACCATGATGAGATTGATTTTGAGTTCTTGGGGAACGTGACTGGAGAGCCTTATATTGTGCATACCAATGTGTACTCCCAAGGGAAAGGTGGTAGAGAGCAACAATTCTACCTATGGTTCGACCCAACAAAAAATTTTCACACTTATACTTTTCTTTGGAACCCCCAACGCATAATATTCTTAGTGGATAACATCCCGATAAGAGTATTCAACAACGGGGAATCTTATGGGGTTCCGTTCCCAAAGAGCAAGCCCATGAAGCTCTACTCAAGCTTGTGGAATGCAGATCAATGGGCTACAAGAGGAGGGCTAGTGAAAACAGATTGGTCTAAAGCTCCTTTCACAGCCTACTACAAAAACTTCAATGCCAATGCCTGTGTGTGGTCTGGTGGATCATCTTCATGTTCATCTACAGGAAACATAGAAAAAGGCTCCAAAAATAATGCTTGGCAGACTCAAAGCCTTGATGCAATTAGTCGCAGAAGACTCAGATGGGTGCAAAAATACTTCATGATTTACAACTATTGCTCTGATTTGAAGCGATTTCCTCGTGGCCGTCCAAGGGAGTGCAACCGTTCTAGGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_16 XET_C

Protein Analysis

286

Amino Acids

32.51

Weight (kDa)

9.43

Isoelectric Point (pI)

44.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000406)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23730 AT4G14130
fragaria_vesca FvH4_3g06510 FvH4_4g12960 FvH4_6g38150 FvH4_6g38150 FvH4_6g38150 FvH4_6g38160 FvH4_6g38170 FvH4_6g38181 FvH4_6g38190
malus_domestica MD04G1020100.v1.1 MD09G1152400.v1.1 MD09G1152600.v1.1 MD09G1152700.v1.1 MD17G1139900.v1.1 MD17G1140000.v1.1
prunus_persica Prupe.1G169700_v2.0.a1 Prupe.3G171500_v2.0.a1 Prupe.3G171600_v2.0.a1 Prupe.3G171700_v2.0.a1 Prupe.3G171800_v2.0.a1 Prupe.3G172000_v2.0.a1 Prupe.3G172100_v2.0.a1 Prupe.4G072800_v2.0.a1
pyrus_communis pycom04g01620 pycom09g07160 pycom09g07220 pycom09g07230 pycom10g22480 pycom13g29190 pycom17g13320
rosa_chinensis RchiOBHm_Chr2g0151371 RchiOBHm_Chr2g0151381 RchiOBHm_Chr2g0151401 RchiOBHm_Chr2g0151421 RchiOBHm_Chr2g0151431 RchiOBHm_Chr2g0151441 RchiOBHm_Chr2g0152071 RchiOBHm_Chr4g0412651 RchiOBHm_Chr5g0012671
rosa_laevigata RLG00000008270 RLG00000020544 RLG00000020546 RLG00000020547 RLG00000020548 RLG00000020549 RLG00000020605 RLG00000031947
rosa_multiflora Rmu_co8167812.1_g000001 Rmu_co8363165.1_g000001 Rmu_co8513985.1_g000001 Rmu_sc0000940.1_g000002 Rmu_sc0000940.1_g000005 Rmu_sc0000940.1_g000006 Rmu_sc0000940.1_g000009 Rmu_sc0002516.1_g000015 Rmu_sc0002516.1_g000017 Rmu_sc0002516.1_g000018 Rmu_sc0043125.1_g000001
rosa_roxburghii Rroxscaffold_1G00063930 Rroxscaffold_2G00095990 Rroxscaffold_2G00096470 Rroxscaffold_2G00096480 Rroxscaffold_2G00096490 Rroxscaffold_2G00096500 Rroxscaffold_5G00356830
rosa_rugosa Rorug02G0425600 Rorug02G0425700 Rorug02G0425800 Rorug02G0425900 Rorug02G0430500 Rorug02G0430600 Rorug04G0115400 Rorug05G0003700
rosa_samantha Rh2BG499200 Rh2CG472700 Rh2CG472800 Rh2CG473000 Rh2CG473100 Rh2CG473300 Rh2CG477800 Rh2DG509800 Rh2DG509900 Rh2DG510100 Rh2DG510200 Rh2DG510300 Rh2DG515000 Rh4AG174700 Rh4BG174000 Rh5AG097500
rosa_wichuraiana Rw2G039850 Rw2G039860 Rw2G039870 Rw2G039880 Rw2G039890 Rw2G039900 Rw2G040400 Rw4G014650 Rw5G008540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 233
AciI CCGC 1 cut(s) 228
AclWI GGATC 1 cut(s) 670
AcoI YGGCCR 1 cut(s) 823
AcsI RAATTY 1 cut(s) 403
AfaI GTAC 1 cut(s) 344
AfiI CCNNNNNNNGG 3 cut(s) 82, 651, 832
AgsI TTSAA 3 cut(s) 481, 634, 808
AjnI CCWGG 1 cut(s) 141
AloI GAACNNNNNNTCC 2 cut(s) 105, 137
AluBI AGCT 7 cut(s) 50, 130, 205, 211, 532, 543, 605
AluI AGCT 7 cut(s) 50, 130, 205, 211, 532, 543, 605
AlwI GGATC 1 cut(s) 670
AoxI GGCC 3 cut(s) 144, 266, 823
ApeKI GCWGC 2 cut(s) 127, 202
ApoI RAATTY 1 cut(s) 403
AspS9I GGNCC 1 cut(s) 266
AsuHPI GGTGA 1 cut(s) 98
BanI GGYRCC 1 cut(s) 233
BauI CACGAG 1 cut(s) 819
BbsI GAAGAC 2 cut(s) 249, 760
BbvI GCAGC 2 cut(s) 139, 214
BccI CCATC 2 cut(s) 99, 756
BceAI ACGGC 1 cut(s) 810
BciT130I CCWGG 1 cut(s) 143
BfaI CTAG 3 cut(s) 581, 849, 856
BfmI CTRYAG 2 cut(s) 125, 681
BisI GCNGC 2 cut(s) 128, 203
BlsI GCNGC 2 cut(s) 129, 204
Bme1390I CCNGG 1 cut(s) 143
BmgT120I GGNCC 1 cut(s) 266
BmiI GGNNCC 4 cut(s) 235, 431, 504, 703
BmrFI CCNGG 1 cut(s) 143
BmsI GCATC 2 cut(s) 53, 727
BpiI GAAGAC 2 cut(s) 249, 760
BpmI CTGGAG 1 cut(s) 333
BpuEI CTTGAG 2 cut(s) 191, 523
BsaJI CCNNGG 2 cut(s) 349, 830
BsaXI ACNNNNNCTCC 4 cut(s) 105, 135, 567, 597
Bsc4I CCNNNNNNNGG 3 cut(s) 82, 651, 832
Bse118I RCCGGY 1 cut(s) 230
Bse1I ACTGG 1 cut(s) 316
Bse3DI GCAATG 1 cut(s) 124
BseBI CCWGG 1 cut(s) 143
BseDI CCNNGG 2 cut(s) 349, 830
BseGI GGATG 1 cut(s) 462
BseLI CCNNNNNNNGG 3 cut(s) 82, 651, 832
BseMI GCAATG 1 cut(s) 124
BseMII CTCAG 1 cut(s) 772
BseNI ACTGG 1 cut(s) 316
BseRI GAGGAG 1 cut(s) 588
BseXI GCAGC 2 cut(s) 139, 214
BshFI GGCC 3 cut(s) 146, 268, 825
BshNI GGYRCC 1 cut(s) 233
BsiSI CCGG 1 cut(s) 231
BslI CCNNNNNNNGG 3 cut(s) 82, 651, 832
BsmI GAATGC 1 cut(s) 556
BsnI GGCC 3 cut(s) 146, 268, 825
Bsp143I GATC 2 cut(s) 556, 662
BspACI CCGC 1 cut(s) 228
BspANI GGCC 3 cut(s) 146, 268, 825
BspCNI CTCAG 1 cut(s) 771
BspHI TCATGA 1 cut(s) 780
BspLI GGNNCC 4 cut(s) 235, 431, 504, 703
BspMAI CTGCAG 1 cut(s) 129
BspPI GGATC 1 cut(s) 670
BspT107I GGYRCC 1 cut(s) 233
BsrDI GCAATG 1 cut(s) 124
BsrFI RCCGGY 1 cut(s) 230
BsrI ACTGG 1 cut(s) 316
BssAI RCCGGY 1 cut(s) 230
BssECI CCNNGG 2 cut(s) 349, 830
BssMI GATC 2 cut(s) 556, 662
BssSI CACGAG 1 cut(s) 819
BssT1I CCWWGG 2 cut(s) 349, 830
Bst2BI CACGAG 1 cut(s) 819
Bst2UI CCWGG 1 cut(s) 143
Bst4CI ACNGT 2 cut(s) 238, 845
BstC8I GCNNGC 3 cut(s) 52, 232, 521
BstDEI CTNAG 2 cut(s) 451, 758
BstEII GGTNACC 1 cut(s) 86
BstF5I GGATG 1 cut(s) 462
BstKTI GATC 2 cut(s) 559, 665
BstMBI GATC 2 cut(s) 556, 662
BstMWI GCNNNNNNNGC 2 cut(s) 208, 529
BstNI CCWGG 1 cut(s) 143
BstPI GGTNACC 1 cut(s) 86
BstSCI CCNGG 1 cut(s) 141
BstSFI CTRYAG 2 cut(s) 125, 681
BstV1I GCAGC 2 cut(s) 139, 214
BstV2I GAAGAC 2 cut(s) 249, 760
BstXI CCANNNNNNTGG 1 cut(s) 149
BsuRI GGCC 3 cut(s) 146, 268, 825
BtsCI GGATG 1 cut(s) 462
BtsI GCAGTG 1 cut(s) 240
BtsIMutI CAGTG 1 cut(s) 240
Cac8I GCNNGC 3 cut(s) 52, 232, 521
CciI TCATGA 1 cut(s) 780
Cfr10I RCCGGY 1 cut(s) 230
Cfr13I GGNCC 1 cut(s) 266
Csp6I GTAC 1 cut(s) 343
CviAII CATG 4 cut(s) 275, 526, 672, 781
CviQI GTAC 1 cut(s) 343
DdeI CTNAG 2 cut(s) 451, 758
DpnI GATC 2 cut(s) 558, 664
DpnII GATC 2 cut(s) 556, 662
EaeI YGGCCR 1 cut(s) 823
EciI GGCGGA 1 cut(s) 217
Eco130I CCWWGG 2 cut(s) 349, 830
Eco147I AGGCCT 1 cut(s) 146
Eco91I GGTNACC 1 cut(s) 86
EcoO65I GGTNACC 1 cut(s) 86
EcoRII CCWGG 1 cut(s) 141
EcoT14I CCWWGG 2 cut(s) 349, 830
ErhI CCWWGG 2 cut(s) 349, 830
FaeI CATG 4 cut(s) 278, 529, 675, 784
FatI CATG 4 cut(s) 274, 525, 671, 780
Fnu4HI GCNGC 2 cut(s) 128, 203
FokI GGATG 1 cut(s) 449
Fsp4HI GCNGC 2 cut(s) 128, 203
FspBI CTAG 3 cut(s) 581, 849, 856
GluI GCNGC 2 cut(s) 128, 203
GsuI CTGGAG 1 cut(s) 333
HaeIII GGCC 3 cut(s) 146, 268, 825
HapII CCGG 1 cut(s) 231
Hin1II CATG 4 cut(s) 278, 529, 675, 784
HindIII AAGCTT 2 cut(s) 209, 541
HinfI GANTC 3 cut(s) 491, 724, 756
HpaII CCGG 1 cut(s) 231
HphI GGTGA 1 cut(s) 98
Hpy166II GTNNAC 1 cut(s) 343
Hpy188I TCNGA 3 cut(s) 262, 761, 802
Hpy188III TCNNGA 2 cut(s) 466, 781
Hpy8I GTNNAC 1 cut(s) 343
HpyAV CCTTC 1 cut(s) 257
HpyCH4III ACNGT 2 cut(s) 238, 845
HpyCH4IV ACGT 1 cut(s) 306
HpyCH4V TGCA 8 cut(s) 127, 202, 218, 331, 554, 740, 769, 840
HpyF10VI GCNNNNNNNGC 2 cut(s) 208, 529
HpyF3I CTNAG 2 cut(s) 451, 758
HpySE526I ACGT 1 cut(s) 306
Hsp92II CATG 4 cut(s) 278, 529, 675, 784
KroI GCCGGC 1 cut(s) 230
KroNI GCCGGC 1 cut(s) 232
Kzo9I GATC 2 cut(s) 556, 662
LmnI GCTCC 2 cut(s) 610, 707
Lsp1109I GCAGC 2 cut(s) 139, 214
LweI GCATC 2 cut(s) 53, 727
MaeI CTAG 3 cut(s) 581, 849, 856
MaeII ACGT 1 cut(s) 306
MaeIII GTNAC 3 cut(s) 86, 238, 307
MalI GATC 2 cut(s) 558, 664
MboI GATC 2 cut(s) 556, 662
MboII GAAGA 3 cut(s) 249, 660, 765
MluCI AATT 4 cut(s) 55, 374, 403, 741
MlyI GAGTC 2 cut(s) 718, 750
MmeI TCCRAC 1 cut(s) 85
MnlI CCTC 6 cut(s) 26, 107, 157, 566, 569, 828
MroNI GCCGGC 1 cut(s) 230
MslI CAYNNNNRTG 2 cut(s) 336, 670
MspA1I CMGCKG 1 cut(s) 50
MspI CCGG 1 cut(s) 231
MspR9I CCNGG 1 cut(s) 143
Mva1269I GAATGC 1 cut(s) 556
MvaI CCWGG 1 cut(s) 143
MwoI GCNNNNNNNGC 2 cut(s) 208, 529
NaeI GCCGGC 1 cut(s) 232
NdeII GATC 2 cut(s) 556, 662
NgoMIV GCCGGC 1 cut(s) 230
NlaIII CATG 4 cut(s) 278, 529, 675, 784
NlaIV GGNNCC 4 cut(s) 235, 431, 504, 703
NmuCI GTSAC 3 cut(s) 86, 238, 307
PagI TCATGA 1 cut(s) 780
PceI AGGCCT 1 cut(s) 146
PctI GAATGC 1 cut(s) 556
PdiI GCCGGC 1 cut(s) 232
PfeI GAWTC 1 cut(s) 491
PkrI GCNGC 2 cut(s) 129, 204
PleI GAGTC 2 cut(s) 718, 750
PpsI GAGTC 2 cut(s) 718, 750
Psp6I CCWGG 1 cut(s) 141
PspEI GGTNACC 1 cut(s) 86
PspGI CCWGG 1 cut(s) 141
PspN4I GGNNCC 4 cut(s) 235, 431, 504, 703
PspPI GGNCC 1 cut(s) 266
PstI CTGCAG 1 cut(s) 129
PvuII CAGCTG 1 cut(s) 50
RsaI GTAC 1 cut(s) 344
RsaNI GTAC 1 cut(s) 343
RseI CAYNNNNRTG 2 cut(s) 336, 670
SatI GCNGC 2 cut(s) 128, 203
Sau3AI GATC 2 cut(s) 556, 662
Sau96I GGNCC 1 cut(s) 266
SchI GAGTC 2 cut(s) 718, 750
ScrFI CCNGG 1 cut(s) 143
SfaNI GCATC 2 cut(s) 53, 727
SfcI CTRYAG 2 cut(s) 125, 681
SmiMI CAYNNNNRTG 2 cut(s) 336, 670
SmlI CTYRAG 2 cut(s) 206, 538
SmoI CTYRAG 2 cut(s) 206, 538
Sse9I AATT 4 cut(s) 55, 374, 403, 741
SseBI AGGCCT 1 cut(s) 146
SsiI CCGC 1 cut(s) 228
SspI AATATT 1 cut(s) 447
SspMI CTAG 3 cut(s) 581, 849, 856
StuI AGGCCT 1 cut(s) 146
StyD4I CCNGG 1 cut(s) 141
StyI CCWWGG 2 cut(s) 349, 830
TaaI ACNGT 2 cut(s) 238, 845
TaiI ACGT 1 cut(s) 309
TaqI TCGA 1 cut(s) 390
TasI AATT 4 cut(s) 55, 374, 403, 741
TatI WGTACW 1 cut(s) 342
TfiI GAWTC 1 cut(s) 491
TscAI CASTG 1 cut(s) 247
TseFI GTSAC 3 cut(s) 86, 238, 307
TseI GCWGC 2 cut(s) 127, 202
Tsp45I GTSAC 3 cut(s) 86, 238, 307
TspDTI ATGAA 4 cut(s) 542, 660, 666, 769
TspGWI ACGGA 1 cut(s) 495
TspRI CASTG 1 cut(s) 247
XapI RAATTY 1 cut(s) 403
XcmI CCANNNNNNNNNTGG 1 cut(s) 356
XspI CTAG 3 cut(s) 581, 849, 856
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.