Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 467 |
| AasI | GACNNNNNNGTC | 2 cut(s) | 172, 616 |
| Acc36I | ACCTGC | 1 cut(s) | 785 |
| Acc65I | GGTACC | 2 cut(s) | 254, 288 |
| AccB1I | GGYRCC | 3 cut(s) | 235, 254, 288 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 547, 567 |
| AccI | GTMKAC | 1 cut(s) | 759 |
| AciI | CCGC | 1 cut(s) | 636 |
| AclWI | GGATC | 2 cut(s) | 407, 682 |
| AcoI | YGGCCR | 1 cut(s) | 598 |
| AcuI | CTGAAG | 1 cut(s) | 267 |
| AcyI | GRCGYC | 1 cut(s) | 236 |
| AdeI | CACNNNGTG | 1 cut(s) | 596 |
| AfaI | GTAC | 3 cut(s) | 256, 290, 355 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 157, 447, 547, 567 |
| AgsI | TTSAA | 2 cut(s) | 502, 821 |
| AjnI | CCWGG | 1 cut(s) | 156 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 381, 413 |
| AluBI | AGCT | 3 cut(s) | 232, 582, 626 |
| AluI | AGCT | 3 cut(s) | 232, 582, 626 |
| Alw21I | GWGCWC | 1 cut(s) | 121 |
| Alw26I | GTCTC | 1 cut(s) | 624 |
| AlwI | GGATC | 2 cut(s) | 407, 682 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 567 |
| Ama87I | CYCGRG | 1 cut(s) | 239 |
| AoxI | GGCC | 2 cut(s) | 63, 598 |
| ApeKI | GCWGC | 2 cut(s) | 682, 791 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 516 |
| Asp718I | GGTACC | 2 cut(s) | 254, 288 |
| AspLEI | GCGC | 1 cut(s) | 238 |
| AspS9I | GGNCC | 2 cut(s) | 160, 174 |
| AsuC2I | CCSGG | 5 cut(s) | 172, 240, 241, 334, 870 |
| AsuHPI | GGTGA | 5 cut(s) | 119, 139, 253, 587, 625 |
| AvaI | CYCGRG | 1 cut(s) | 239 |
| AvaII | GGWCC | 2 cut(s) | 160, 174 |
| BanI | GGYRCC | 3 cut(s) | 235, 254, 288 |
| BbsI | GAAGAC | 1 cut(s) | 614 |
| Bbv12I | GWGCWC | 1 cut(s) | 121 |
| BbvI | GCAGC | 2 cut(s) | 694, 778 |
| BccI | CCATC | 5 cut(s) | 125, 300, 408, 566, 807 |
| BceAI | ACGGC | 1 cut(s) | 585 |
| BciT130I | CCWGG | 1 cut(s) | 158 |
| BcnI | CCSGG | 5 cut(s) | 172, 240, 241, 334, 870 |
| BcoDI | GTCTC | 1 cut(s) | 624 |
| BfaI | CTAG | 4 cut(s) | 72, 671, 779, 907 |
| BfmI | CTRYAG | 1 cut(s) | 792 |
| BfoI | RGCGCY | 1 cut(s) | 239 |
| BfuAI | ACCTGC | 1 cut(s) | 785 |
| BisI | GCNGC | 2 cut(s) | 683, 792 |
| BlsI | GCNGC | 2 cut(s) | 684, 793 |
| BmcAI | AGTACT | 1 cut(s) | 355 |
| Bme1390I | CCNGG | 6 cut(s) | 158, 172, 240, 241, 334, 870 |
| Bme18I | GGWCC | 2 cut(s) | 160, 174 |
| BmeT110I | CYCGRG | 1 cut(s) | 239 |
| BmgT120I | GGNCC | 2 cut(s) | 160, 174 |
| BmiI | GGNNCC | 4 cut(s) | 175, 237, 256, 290 |
| BmrFI | CCNGG | 6 cut(s) | 158, 172, 240, 241, 334, 870 |
| BpiI | GAAGAC | 1 cut(s) | 614 |
| BpuEI | CTTGAG | 1 cut(s) | 606 |
| BpuMI | CCSGG | 5 cut(s) | 172, 240, 241, 334, 870 |
| BsaHI | GRCGYC | 1 cut(s) | 236 |
| BsaI | GGTCTC | 1 cut(s) | 624 |
| BsaJI | CCNNGG | 4 cut(s) | 156, 239, 546, 881 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 422, 452 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 157, 447, 547, 567 |
| Bse1I | ACTGG | 2 cut(s) | 561, 620 |
| BseBI | CCWGG | 1 cut(s) | 158 |
| BseDI | CCNNGG | 4 cut(s) | 156, 239, 546, 881 |
| BseGI | GGATG | 3 cut(s) | 18, 329, 577 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 157, 447, 547, 567 |
| BseMII | CTCAG | 2 cut(s) | 203, 364 |
| BseNI | ACTGG | 2 cut(s) | 561, 620 |
| BseXI | GCAGC | 2 cut(s) | 694, 778 |
| BseYI | CCCAGC | 1 cut(s) | 582 |
| BsgI | GTGCAG | 1 cut(s) | 832 |
| BshFI | GGCC | 2 cut(s) | 65, 600 |
| BshNI | GGYRCC | 3 cut(s) | 235, 254, 288 |
| BsiHKAI | GWGCWC | 1 cut(s) | 121 |
| BsiHKCI | CYCGRG | 1 cut(s) | 239 |
| BsiSI | CCGG | 5 cut(s) | 171, 177, 240, 333, 870 |
| BslFI | GGGAC | 1 cut(s) | 471 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 157, 447, 547, 567 |
| BsmAI | GTCTC | 1 cut(s) | 624 |
| BsmFI | GGGAC | 1 cut(s) | 471 |
| BsmI | GAATGC | 2 cut(s) | 893, 895 |
| BsnI | GGCC | 2 cut(s) | 65, 600 |
| Bso31I | GGTCTC | 1 cut(s) | 624 |
| BsoBI | CYCGRG | 1 cut(s) | 239 |
| Bsp1286I | GDGCHC | 1 cut(s) | 121 |
| Bsp143I | GATC | 3 cut(s) | 412, 674, 834 |
| Bsp19I | CCATGG | 1 cut(s) | 546 |
| BspACI | CCGC | 1 cut(s) | 636 |
| BspANI | GGCC | 2 cut(s) | 65, 600 |
| BspCNI | CTCAG | 2 cut(s) | 202, 363 |
| BspHI | TCATGA | 2 cut(s) | 295, 831 |
| BspLI | GGNNCC | 4 cut(s) | 175, 237, 256, 290 |
| BspMAI | CTGCAG | 1 cut(s) | 796 |
| BspMI | ACCTGC | 1 cut(s) | 785 |
| BspPI | GGATC | 2 cut(s) | 407, 682 |
| BspT107I | GGYRCC | 3 cut(s) | 235, 254, 288 |
| BspTNI | GGTCTC | 1 cut(s) | 624 |
| BsrI | ACTGG | 2 cut(s) | 561, 620 |
| BssECI | CCNNGG | 4 cut(s) | 156, 239, 546, 881 |
| BssMI | GATC | 3 cut(s) | 412, 674, 834 |
| BssNI | GRCGYC | 1 cut(s) | 236 |
| BssT1I | CCWWGG | 1 cut(s) | 546 |
| Bst2UI | CCWGG | 1 cut(s) | 158 |
| Bst4CI | ACNGT | 5 cut(s) | 213, 259, 485, 728, 857 |
| BstACI | GRCGYC | 1 cut(s) | 236 |
| BstC8I | GCNNGC | 2 cut(s) | 234, 893 |
| BstDEI | CTNAG | 2 cut(s) | 189, 350 |
| BstDSI | CCRYGG | 2 cut(s) | 546, 881 |
| BstEII | GGTNACC | 1 cut(s) | 107 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 155 |
| BstF5I | GGATG | 3 cut(s) | 18, 329, 577 |
| BstH2I | RGCGCY | 1 cut(s) | 239 |
| BstHHI | GCGC | 1 cut(s) | 238 |
| BstKTI | GATC | 3 cut(s) | 415, 677, 837 |
| BstMAI | GTCTC | 1 cut(s) | 624 |
| BstMBI | GATC | 3 cut(s) | 412, 674, 834 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 229, 557 |
| BstNI | CCWGG | 1 cut(s) | 158 |
| BstNSI | RCATGY | 1 cut(s) | 223 |
| BstPI | GGTNACC | 1 cut(s) | 107 |
| BstSCI | CCNGG | 6 cut(s) | 156, 170, 238, 239, 332, 868 |
| BstSFI | CTRYAG | 1 cut(s) | 792 |
| BstV1I | GCAGC | 2 cut(s) | 694, 778 |
| BstV2I | GAAGAC | 1 cut(s) | 614 |
| BsuRI | GGCC | 2 cut(s) | 65, 600 |
| BtgI | CCRYGG | 2 cut(s) | 546, 881 |
| BtgZI | GCGATG | 1 cut(s) | 443 |
| BtsCI | GGATG | 3 cut(s) | 18, 329, 577 |
| BtsI | GCAGTG | 1 cut(s) | 246 |
| BtsIMutI | CAGTG | 2 cut(s) | 246, 849 |
| BveI | ACCTGC | 1 cut(s) | 785 |
| Cac8I | GCNNGC | 2 cut(s) | 234, 893 |
| CaiI | CAGNNNCTG | 1 cut(s) | 567 |
| CciI | TCATGA | 2 cut(s) | 295, 831 |
| CfoI | GCGC | 1 cut(s) | 238 |
| Cfr13I | GGNCC | 2 cut(s) | 160, 174 |
| Cfr9I | CCCGGG | 1 cut(s) | 239 |
| Csp6I | GTAC | 3 cut(s) | 255, 289, 354 |
| CviAII | CATG | 7 cut(s) | 52, 220, 296, 521, 547, 768, 832 |
| CviQI | GTAC | 3 cut(s) | 255, 289, 354 |
| DdeI | CTNAG | 2 cut(s) | 189, 350 |
| DinI | GGCGCC | 1 cut(s) | 237 |
| DpnI | GATC | 3 cut(s) | 414, 676, 836 |
| DpnII | GATC | 3 cut(s) | 412, 674, 834 |
| DraIII | CACNNNGTG | 1 cut(s) | 596 |
| DrdI | GACNNNNNNGTC | 2 cut(s) | 172, 616 |
| DseDI | GACNNNNNNGTC | 2 cut(s) | 172, 616 |
| EaeI | YGGCCR | 1 cut(s) | 598 |
| Eco130I | CCWWGG | 1 cut(s) | 546 |
| Eco31I | GGTCTC | 1 cut(s) | 624 |
| Eco47I | GGWCC | 2 cut(s) | 160, 174 |
| Eco57I | CTGAAG | 1 cut(s) | 267 |
| Eco88I | CYCGRG | 1 cut(s) | 239 |
| Eco91I | GGTNACC | 1 cut(s) | 107 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 155 |
| EcoO65I | GGTNACC | 1 cut(s) | 107 |
| EcoRII | CCWGG | 1 cut(s) | 156 |
| EcoT14I | CCWWGG | 1 cut(s) | 546 |
| EgeI | GGCGCC | 1 cut(s) | 237 |
| EheI | GGCGCC | 1 cut(s) | 237 |
| ErhI | CCWWGG | 1 cut(s) | 546 |
| FaeI | CATG | 7 cut(s) | 55, 223, 299, 524, 550, 771, 835 |
| FaqI | GGGAC | 1 cut(s) | 471 |
| FatI | CATG | 7 cut(s) | 51, 219, 295, 520, 546, 767, 831 |
| FblI | GTMKAC | 1 cut(s) | 759 |
| Fnu4HI | GCNGC | 2 cut(s) | 683, 792 |
| FokI | GGATG | 3 cut(s) | 5, 316, 584 |
| Fsp4HI | GCNGC | 2 cut(s) | 683, 792 |
| FspBI | CTAG | 4 cut(s) | 72, 671, 779, 907 |
| GlaI | GCGC | 1 cut(s) | 237 |
| GluI | GCNGC | 2 cut(s) | 683, 792 |
| GsaI | CCCAGC | 1 cut(s) | 586 |
| HaeII | RGCGCY | 1 cut(s) | 239 |
| HaeIII | GGCC | 2 cut(s) | 65, 600 |
| HapII | CCGG | 5 cut(s) | 171, 177, 240, 333, 870 |
| HhaI | GCGC | 1 cut(s) | 238 |
| Hin1I | GRCGYC | 1 cut(s) | 236 |
| Hin1II | CATG | 7 cut(s) | 55, 223, 299, 524, 550, 771, 835 |
| Hin6I | GCGC | 1 cut(s) | 236 |
| HinP1I | GCGC | 1 cut(s) | 236 |
| HincII | GTYRAC | 1 cut(s) | 760 |
| HindII | GTYRAC | 1 cut(s) | 760 |
| HinfI | GANTC | 2 cut(s) | 337, 775 |
| HpaII | CCGG | 5 cut(s) | 171, 177, 240, 333, 870 |
| HphI | GGTGA | 5 cut(s) | 119, 139, 253, 587, 625 |
| Hpy166II | GTNNAC | 1 cut(s) | 760 |
| Hpy188I | TCNGA | 1 cut(s) | 192 |
| Hpy188III | TCNNGA | 3 cut(s) | 86, 296, 832 |
| Hpy8I | GTNNAC | 1 cut(s) | 760 |
| HpyCH4III | ACNGT | 5 cut(s) | 213, 259, 485, 728, 857 |
| HpyCH4IV | ACGT | 1 cut(s) | 115 |
| HpyCH4V | TGCA | 3 cut(s) | 223, 794, 849 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 229, 557 |
| HpyF3I | CTNAG | 2 cut(s) | 189, 350 |
| HpySE526I | ACGT | 1 cut(s) | 115 |
| Hsp92I | GRCGYC | 1 cut(s) | 236 |
| Hsp92II | CATG | 7 cut(s) | 55, 223, 299, 524, 550, 771, 835 |
| HspAI | GCGC | 1 cut(s) | 236 |
| KasI | GGCGCC | 1 cut(s) | 235 |
| KpnI | GGTACC | 2 cut(s) | 258, 292 |
| Kzo9I | GATC | 3 cut(s) | 412, 674, 834 |
| LmnI | GCTCC | 1 cut(s) | 631 |
| Lsp1109I | GCAGC | 2 cut(s) | 694, 778 |
| MaeI | CTAG | 4 cut(s) | 72, 671, 779, 907 |
| MaeII | ACGT | 1 cut(s) | 115 |
| MaeIII | GTNAC | 2 cut(s) | 107, 259 |
| MalI | GATC | 3 cut(s) | 414, 676, 836 |
| MboI | GATC | 3 cut(s) | 412, 674, 834 |
| MboII | GAAGA | 3 cut(s) | 273, 619, 660 |
| MhlI | GDGCHC | 1 cut(s) | 121 |
| MluCI | AATT | 3 cut(s) | 76, 395, 745 |
| Mly113I | GGCGCC | 1 cut(s) | 236 |
| MnlI | CCTC | 6 cut(s) | 76, 128, 161, 427, 649, 765 |
| MroXI | GAANNNNTTC | 1 cut(s) | 516 |
| MseI | TTAA | 1 cut(s) | 786 |
| MslI | CAYNNNNRTG | 3 cut(s) | 224, 297, 886 |
| MspI | CCGG | 5 cut(s) | 171, 177, 240, 333, 870 |
| MspR9I | CCNGG | 6 cut(s) | 158, 172, 240, 241, 334, 870 |
| Mva1269I | GAATGC | 2 cut(s) | 893, 895 |
| MvaI | CCWGG | 1 cut(s) | 158 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 229, 557 |
| NarI | GGCGCC | 1 cut(s) | 236 |
| NciI | CCSGG | 5 cut(s) | 172, 240, 241, 334, 870 |
| NcoI | CCATGG | 1 cut(s) | 546 |
| NdeII | GATC | 3 cut(s) | 412, 674, 834 |
| NlaIII | CATG | 7 cut(s) | 55, 223, 299, 524, 550, 771, 835 |
| NlaIV | GGNNCC | 4 cut(s) | 175, 237, 256, 290 |
| NmuCI | GTSAC | 2 cut(s) | 107, 259 |
| NspI | RCATGY | 1 cut(s) | 223 |
| PagI | TCATGA | 2 cut(s) | 295, 831 |
| PctI | GAATGC | 2 cut(s) | 893, 895 |
| PdmI | GAANNNNTTC | 1 cut(s) | 516 |
| PfeI | GAWTC | 2 cut(s) | 337, 775 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 547, 567 |
| PfoI | TCCNGGA | 1 cut(s) | 332 |
| PkrI | GCNGC | 2 cut(s) | 684, 793 |
| PluTI | GGCGCC | 1 cut(s) | 239 |
| PsiI | TTATAA | 1 cut(s) | 467 |
| Psp6I | CCWGG | 1 cut(s) | 156 |
| PspEI | GGTNACC | 1 cut(s) | 107 |
| PspFI | CCCAGC | 1 cut(s) | 582 |
| PspGI | CCWGG | 1 cut(s) | 156 |
| PspN4I | GGNNCC | 4 cut(s) | 175, 237, 256, 290 |
| PspPI | GGNCC | 2 cut(s) | 160, 174 |
| PstI | CTGCAG | 1 cut(s) | 796 |
| PstNI | CAGNNNCTG | 1 cut(s) | 567 |
| RsaI | GTAC | 3 cut(s) | 256, 290, 355 |
| RsaNI | GTAC | 3 cut(s) | 255, 289, 354 |
| RseI | CAYNNNNRTG | 3 cut(s) | 224, 297, 886 |
| SalI | GTCGAC | 1 cut(s) | 758 |
| SaqAI | TTAA | 1 cut(s) | 786 |
| SatI | GCNGC | 2 cut(s) | 683, 792 |
| Sau3AI | GATC | 3 cut(s) | 412, 674, 834 |
| Sau96I | GGNCC | 2 cut(s) | 160, 174 |
| ScaI | AGTACT | 1 cut(s) | 355 |
| ScrFI | CCNGG | 6 cut(s) | 158, 172, 240, 241, 334, 870 |
| SduI | GDGCHC | 1 cut(s) | 121 |
| SetI | ASST | 8 cut(s) | 118, 204, 234, 584, 598, 628, 691, 799 |
| SfcI | CTRYAG | 1 cut(s) | 792 |
| SfoI | GGCGCC | 1 cut(s) | 237 |
| SinI | GGWCC | 2 cut(s) | 160, 174 |
| SmaI | CCCGGG | 1 cut(s) | 241 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 224, 297, 886 |
| SmlI | CTYRAG | 1 cut(s) | 621 |
| SmoI | CTYRAG | 1 cut(s) | 621 |
| Sse9I | AATT | 3 cut(s) | 76, 395, 745 |
| SsiI | CCGC | 1 cut(s) | 636 |
| SspDI | GGCGCC | 1 cut(s) | 235 |
| SspMI | CTAG | 4 cut(s) | 72, 671, 779, 907 |
| StyD4I | CCNGG | 6 cut(s) | 156, 170, 238, 239, 332, 868 |
| StyI | CCWWGG | 1 cut(s) | 546 |
| TaaI | ACNGT | 5 cut(s) | 213, 259, 485, 728, 857 |
| TaiI | ACGT | 1 cut(s) | 118 |
| TaqI | TCGA | 4 cut(s) | 216, 759, 799, 811 |
| TasI | AATT | 3 cut(s) | 76, 395, 745 |
| TatI | WGTACW | 1 cut(s) | 353 |
| TfiI | GAWTC | 2 cut(s) | 337, 775 |
| Tru1I | TTAA | 1 cut(s) | 786 |
| Tru9I | TTAA | 1 cut(s) | 786 |
| TscAI | CASTG | 2 cut(s) | 253, 856 |
| TseFI | GTSAC | 2 cut(s) | 107, 259 |
| TseI | GCWGC | 2 cut(s) | 682, 791 |
| Tsp45I | GTSAC | 2 cut(s) | 107, 259 |
| TspDTI | ATGAA | 7 cut(s) | 28, 172, 242, 446, 509, 681, 820 |
| TspGWI | ACGGA | 2 cut(s) | 430, 516 |
| TspMI | CCCGGG | 1 cut(s) | 239 |
| TspRI | CASTG | 2 cut(s) | 253, 856 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 547, 567 |
| VpaK11BI | GGWCC | 2 cut(s) | 160, 174 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 155 |
| XceI | RCATGY | 1 cut(s) | 223 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 203 |
| XmaI | CCCGGG | 1 cut(s) | 239 |
| XmiI | GTMKAC | 1 cut(s) | 759 |
| XmnI | GAANNNNTTC | 1 cut(s) | 516 |
| XspI | CTAG | 4 cut(s) | 72, 671, 779, 907 |
| ZrmI | AGTACT | 1 cut(s) | 355 |