Rw4G014650

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000406
Physical Location & Seq
Forward (+)
33946775 .. 33949092
2318 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G014650.1

Sequence Viewer

Length: 846 bp
ATGGGGATTTCTGGTAACGTGTCTTGTCTGGTTTTGTTGGTTGGTTTAGCATTGAGCTCTTTAATAGTAGGCTCTTATGCTGGCAACTTCTATCAAGACTTTGACTTGACATGGGGTGGTCACCGTCCTAAGATATTCAAGGGAGGTCAGCTTCTTTCTTTGTCTTTGGACAAGGTCTCTGGCTCTGGATTCCAGTCCAAGAAAGAGTACCTGTTCGGGAGGATTGACATGCAACTTAAGCTTGTTGCCGGAAACTCTGCCGGTACCGTGACTGCCTACTACTTGTCTTCTCAAGGCCCTACACATGATGAAATCGACTTTGAATTCTTGGGAAATGTCAGTGGAGATCCATATGTGTTGCATACCAATATTTTCACTCAGGGCAAGGGAAACAGAGAGCAGCAATTCTATCTCTGGTTTGACCCCTCAAAAAACTTCCACACTTACTCCATCATCTGGAAGCCCCAGCACATAATTTTCTTGGTGGATAATACTCCCATTAGAGTGTTCAAAAATGCTGAATCACTGGGTGTTCCATTCCCAAAGAACCAAGCCATGAGGATTTACTCGAGCCTTTGGAATGCTGATGATTGGGCTACAAGAGGAGGATTGGTGAAAACTGATTGGTCAAAGGCACCCTTTACAGCATACTACAGAAACTTCAACGTCATCGATGCTAAATCATCCAAATCATTCTCTGATTCTCAGCCTAGTTGGCAGACCAATGCACTTGATGCTCCTAGCCGAAGACGCCTGAGGTGGGTTCAGAAGTACTTCATGATCTACAACTACTGTACCGATTTAAAACGCTTCCCACGAGGTTTTCCTGCTGAGTGTAGGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_16 XET_C

Protein Analysis

281

Amino Acids

31.93

Weight (kDa)

9.37

Isoelectric Point (pI)

38.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 31 - 211 1.1e-66 Glycosyl hydrolases family 16
XET_C PF06955 235 - 279 3.6e-18 Xyloglucan endo-transglycosylase (XET) C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000406)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23730 AT4G14130
fragaria_vesca FvH4_3g06510 FvH4_4g12960 FvH4_6g38150 FvH4_6g38150 FvH4_6g38150 FvH4_6g38160 FvH4_6g38170 FvH4_6g38181 FvH4_6g38190
malus_domestica MD04G1020100.v1.1 MD09G1152400.v1.1 MD09G1152600.v1.1 MD09G1152700.v1.1 MD17G1139900.v1.1 MD17G1140000.v1.1
prunus_persica Prupe.1G169700_v2.0.a1 Prupe.3G171500_v2.0.a1 Prupe.3G171600_v2.0.a1 Prupe.3G171700_v2.0.a1 Prupe.3G171800_v2.0.a1 Prupe.3G172000_v2.0.a1 Prupe.3G172100_v2.0.a1 Prupe.4G072800_v2.0.a1
pyrus_communis pycom04g01620 pycom09g07160 pycom09g07220 pycom09g07230 pycom10g22480 pycom13g29190 pycom17g13320
rosa_chinensis RchiOBHm_Chr2g0151371 RchiOBHm_Chr2g0151381 RchiOBHm_Chr2g0151401 RchiOBHm_Chr2g0151421 RchiOBHm_Chr2g0151431 RchiOBHm_Chr2g0151441 RchiOBHm_Chr2g0152071 RchiOBHm_Chr4g0412651 RchiOBHm_Chr5g0012671
rosa_laevigata RLG00000008270 RLG00000020544 RLG00000020546 RLG00000020547 RLG00000020548 RLG00000020549 RLG00000020605 RLG00000031947
rosa_multiflora Rmu_co8167812.1_g000001 Rmu_co8363165.1_g000001 Rmu_co8513985.1_g000001 Rmu_sc0000940.1_g000002 Rmu_sc0000940.1_g000005 Rmu_sc0000940.1_g000006 Rmu_sc0000940.1_g000009 Rmu_sc0002516.1_g000015 Rmu_sc0002516.1_g000017 Rmu_sc0002516.1_g000018 Rmu_sc0043125.1_g000001
rosa_roxburghii Rroxscaffold_1G00063930 Rroxscaffold_2G00095990 Rroxscaffold_2G00096470 Rroxscaffold_2G00096480 Rroxscaffold_2G00096490 Rroxscaffold_2G00096500 Rroxscaffold_5G00356830
rosa_rugosa Rorug02G0425600 Rorug02G0425700 Rorug02G0425800 Rorug02G0425900 Rorug02G0430500 Rorug02G0430600 Rorug04G0115400 Rorug05G0003700
rosa_samantha Rh2BG499200 Rh2CG472700 Rh2CG472800 Rh2CG473000 Rh2CG473100 Rh2CG473300 Rh2CG477800 Rh2DG509800 Rh2DG509900 Rh2DG510100 Rh2DG510200 Rh2DG510300 Rh2DG515000 Rh4AG174700 Rh4BG174000 Rh5AG097500
rosa_wichuraiana Rw2G039850 Rw2G039860 Rw2G039870 Rw2G039880 Rw2G039890 Rw2G039900 Rw2G040400 Rw4G014650 Rw5G008540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 263
AccB1I GGYRCC 2 cut(s) 263, 634
AccB7I CCANNNNNTGG 1 cut(s) 456
AclWI GGATC 1 cut(s) 341
AcsI RAATTY 1 cut(s) 323
AcyI GRCGYC 1 cut(s) 751
AdeI CACNNNGTG 1 cut(s) 530
AfaI GTAC 4 cut(s) 209, 265, 773, 796
AfiI CCNNNNNNNGG 2 cut(s) 456, 760
AflII CTTAAG 1 cut(s) 236
AflIII ACRYGT 1 cut(s) 18
AgsI TTSAA 4 cut(s) 139, 323, 511, 664
AluBI AGCT 3 cut(s) 57, 151, 241
AluI AGCT 3 cut(s) 57, 151, 241
Alw21I GWGCWC 1 cut(s) 59
Alw26I GTCTC 1 cut(s) 181
AlwI GGATC 1 cut(s) 341
Ama87I CYCGRG 1 cut(s) 568
AoxI GGCC 1 cut(s) 295
ApeKI GCWGC 1 cut(s) 400
ApoI RAATTY 1 cut(s) 323
Asp700I GAANNNNTTC 1 cut(s) 773
Asp718I GGTACC 1 cut(s) 263
AspS9I GGNCC 1 cut(s) 296
AsuHPI GGTGA 2 cut(s) 113, 625
AvaI CYCGRG 1 cut(s) 568
AxyI CCTNAGG 1 cut(s) 755
BaeI ACNNNNGTAYC 2 cut(s) 778, 811
BanI GGYRCC 2 cut(s) 263, 634
BanII GRGCYC 1 cut(s) 59
BauI CACGAG 1 cut(s) 816
BbsI GAAGAC 2 cut(s) 279, 754
Bbv12I GWGCWC 1 cut(s) 59
BbvI GCAGC 1 cut(s) 412
BccI CCATC 1 cut(s) 458
BcoDI GTCTC 1 cut(s) 181
BfaI CTAG 2 cut(s) 711, 741
BfmI CTRYAG 1 cut(s) 652
BfrI CTTAAG 1 cut(s) 236
BglI GCCNNNNNGGC 1 cut(s) 715
BisI GCNGC 1 cut(s) 401
BlsI GCNGC 1 cut(s) 402
BmcAI AGTACT 1 cut(s) 773
BmeT110I CYCGRG 1 cut(s) 568
BmgT120I GGNCC 1 cut(s) 296
BmiI GGNNCC 2 cut(s) 265, 636
BmrI ACTGGG 1 cut(s) 536
BmsI GCATC 2 cut(s) 664, 724
BmuI ACTGGG 1 cut(s) 536
BpiI GAAGAC 2 cut(s) 279, 754
BpuEI CTTGAG 1 cut(s) 276
Bsa29I ATCGAT 1 cut(s) 672
BsaHI GRCGYC 1 cut(s) 751
BsaI GGTCTC 1 cut(s) 181
BsaXI ACNNNNNCTCC 4 cut(s) 431, 461, 597, 627
Bsc4I CCNNNNNNNGG 2 cut(s) 456, 760
Bse118I RCCGGY 1 cut(s) 260
Bse1I ACTGG 2 cut(s) 193, 531
Bse21I CCTNAGG 1 cut(s) 755
BseCI ATCGAT 1 cut(s) 672
BseGI GGATG 1 cut(s) 683
BseLI CCNNNNNNNGG 2 cut(s) 456, 760
BseMII CTCAG 4 cut(s) 392, 719, 746, 822
BseNI ACTGG 2 cut(s) 193, 531
BseRI GAGGAG 1 cut(s) 618
BseXI GCAGC 1 cut(s) 412
BseYI CCCAGC 1 cut(s) 465
BshFI GGCC 1 cut(s) 297
BshNI GGYRCC 2 cut(s) 263, 634
BshVI ATCGAT 1 cut(s) 672
BsiHKAI GWGCWC 1 cut(s) 59
BsiHKCI CYCGRG 1 cut(s) 568
BsiSI CCGG 2 cut(s) 249, 261
BslI CCNNNNNNNGG 2 cut(s) 456, 760
BsmAI GTCTC 1 cut(s) 181
BsmI GAATGC 1 cut(s) 586
BsnI GGCC 1 cut(s) 297
Bso31I GGTCTC 1 cut(s) 181
BsoBI CYCGRG 1 cut(s) 568
Bsp1286I GDGCHC 1 cut(s) 59
Bsp143I GATC 2 cut(s) 346, 780
BspANI GGCC 1 cut(s) 297
BspCNI CTCAG 4 cut(s) 391, 718, 747, 823
BspDI ATCGAT 1 cut(s) 672
BspHI TCATGA 1 cut(s) 777
BspLI GGNNCC 2 cut(s) 265, 636
BspPI GGATC 1 cut(s) 341
BspT107I GGYRCC 2 cut(s) 263, 634
BspTI CTTAAG 1 cut(s) 236
BspTNI GGTCTC 1 cut(s) 181
BsrFI RCCGGY 1 cut(s) 260
BsrI ACTGG 2 cut(s) 193, 531
BssAI RCCGGY 1 cut(s) 260
BssMI GATC 2 cut(s) 346, 780
BssNI GRCGYC 1 cut(s) 751
BssSI CACGAG 1 cut(s) 816
Bst2BI CACGAG 1 cut(s) 816
Bst4CI ACNGT 3 cut(s) 125, 268, 794
BstACI GRCGYC 1 cut(s) 751
BstAFI CTTAAG 1 cut(s) 236
BstAPI GCANNNNNTGC 1 cut(s) 734
BstC8I GCNNGC 1 cut(s) 82
BstDEI CTNAG 5 cut(s) 129, 378, 705, 755, 831
BstEII GGTNACC 1 cut(s) 119
BstF5I GGATG 1 cut(s) 683
BstKTI GATC 2 cut(s) 349, 783
BstMAI GTCTC 1 cut(s) 181
BstMBI GATC 2 cut(s) 346, 780
BstMWI GCNNNNNNNGC 4 cut(s) 238, 715, 734, 750
BstNSI RCATGY 1 cut(s) 232
BstPI GGTNACC 1 cut(s) 119
BstSFI CTRYAG 1 cut(s) 652
BstV1I GCAGC 1 cut(s) 412
BstV2I GAAGAC 2 cut(s) 279, 754
BstX2I RGATCY 1 cut(s) 346
BstYI RGATCY 1 cut(s) 346
Bsu15I ATCGAT 1 cut(s) 672
Bsu36I CCTNAGG 1 cut(s) 755
BsuRI GGCC 1 cut(s) 297
BsuTUI ATCGAT 1 cut(s) 672
BtsCI GGATG 1 cut(s) 683
BtsIMutI CAGTG 2 cut(s) 346, 524
Cac8I GCNNGC 1 cut(s) 82
CciI TCATGA 1 cut(s) 777
Cfr10I RCCGGY 1 cut(s) 260
Cfr13I GGNCC 1 cut(s) 296
ClaI ATCGAT 1 cut(s) 672
CseI GACGC 1 cut(s) 759
Csp6I GTAC 4 cut(s) 208, 264, 772, 795
CviAII CATG 5 cut(s) 111, 229, 305, 556, 778
CviQI GTAC 4 cut(s) 208, 264, 772, 795
DdeI CTNAG 5 cut(s) 129, 378, 705, 755, 831
DpnI GATC 2 cut(s) 348, 782
DpnII GATC 2 cut(s) 346, 780
DraI TTTAAA 1 cut(s) 804
DraIII CACNNNGTG 1 cut(s) 530
Ecl136II GAGCTC 1 cut(s) 57
Eco24I GRGCYC 1 cut(s) 59
Eco31I GGTCTC 1 cut(s) 181
Eco53kI GAGCTC 1 cut(s) 57
Eco81I CCTNAGG 1 cut(s) 755
Eco88I CYCGRG 1 cut(s) 568
Eco91I GGTNACC 1 cut(s) 119
EcoICRI GAGCTC 1 cut(s) 57
EcoO109I RGGNCCY 1 cut(s) 296
EcoO65I GGTNACC 1 cut(s) 119
EcoRI GAATTC 1 cut(s) 323
EcoT38I GRGCYC 1 cut(s) 59
FaeI CATG 5 cut(s) 114, 232, 308, 559, 781
FalI AAGNNNNNCTT 2 cut(s) 623, 655
FatI CATG 5 cut(s) 110, 228, 304, 555, 777
FauNDI CATATG 1 cut(s) 352
Fnu4HI GCNGC 1 cut(s) 401
FokI GGATG 1 cut(s) 670
FriOI GRGCYC 1 cut(s) 59
Fsp4HI GCNGC 1 cut(s) 401
FspBI CTAG 2 cut(s) 711, 741
GluI GCNGC 1 cut(s) 401
GsaI CCCAGC 1 cut(s) 469
HaeIII GGCC 1 cut(s) 297
HapII CCGG 2 cut(s) 249, 261
HgaI GACGC 1 cut(s) 759
Hin1I GRCGYC 1 cut(s) 751
Hin1II CATG 5 cut(s) 114, 232, 308, 559, 781
HindIII AAGCTT 1 cut(s) 239
HinfI GANTC 3 cut(s) 189, 521, 701
HpaII CCGG 2 cut(s) 249, 261
HphI GGTGA 2 cut(s) 113, 625
Hpy188I TCNGA 2 cut(s) 700, 768
Hpy188III TCNNGA 5 cut(s) 95, 186, 217, 457, 778
HpyCH4III ACNGT 3 cut(s) 125, 268, 794
HpyCH4IV ACGT 2 cut(s) 18, 666
HpyCH4V TGCA 3 cut(s) 232, 361, 728
HpyF10VI GCNNNNNNNGC 4 cut(s) 238, 715, 734, 750
HpyF3I CTNAG 5 cut(s) 129, 378, 705, 755, 831
HpySE526I ACGT 2 cut(s) 18, 666
Hsp92I GRCGYC 1 cut(s) 751
Hsp92II CATG 5 cut(s) 114, 232, 308, 559, 781
KpnI GGTACC 1 cut(s) 267
Kzo9I GATC 2 cut(s) 346, 780
LmnI GCTCC 1 cut(s) 742
Lsp1109I GCAGC 1 cut(s) 412
LweI GCATC 2 cut(s) 664, 724
MaeI CTAG 2 cut(s) 711, 741
MaeII ACGT 2 cut(s) 18, 666
MaeIII GTNAC 3 cut(s) 14, 119, 268
MalI GATC 2 cut(s) 348, 782
MboI GATC 2 cut(s) 346, 780
MboII GAAGA 2 cut(s) 279, 759
MflI RGATCY 1 cut(s) 346
MhlI GDGCHC 1 cut(s) 59
MluCI AATT 3 cut(s) 323, 404, 474
MnlI CCTC 8 cut(s) 137, 213, 436, 552, 596, 599, 750, 812
MroXI GAANNNNTTC 1 cut(s) 773
MseI TTAA 3 cut(s) 62, 237, 803
MslI CAYNNNNRTG 1 cut(s) 503
MspCI CTTAAG 1 cut(s) 236
MspI CCGG 2 cut(s) 249, 261
Mva1269I GAATGC 1 cut(s) 586
MwoI GCNNNNNNNGC 4 cut(s) 238, 715, 734, 750
NdeI CATATG 1 cut(s) 352
NdeII GATC 2 cut(s) 346, 780
NlaIII CATG 5 cut(s) 114, 232, 308, 559, 781
NlaIV GGNNCC 2 cut(s) 265, 636
NmuCI GTSAC 2 cut(s) 119, 268
NspI RCATGY 1 cut(s) 232
PaeR7I CTCGAG 1 cut(s) 568
PagI TCATGA 1 cut(s) 777
PcsI WCGNNNNNNNCGW 1 cut(s) 814
PctI GAATGC 1 cut(s) 586
PdmI GAANNNNTTC 1 cut(s) 773
PfeI GAWTC 3 cut(s) 189, 521, 701
PflFI GACNNNGTC 1 cut(s) 173
PflMI CCANNNNNTGG 1 cut(s) 456
PkrI GCNGC 1 cut(s) 402
Psp124BI GAGCTC 1 cut(s) 59
PspEI GGTNACC 1 cut(s) 119
PspFI CCCAGC 1 cut(s) 465
PspN4I GGNNCC 2 cut(s) 265, 636
PspPI GGNCC 1 cut(s) 296
PspXI VCTCGAGB 1 cut(s) 568
PsuI RGATCY 1 cut(s) 346
PsyI GACNNNGTC 1 cut(s) 173
RsaI GTAC 4 cut(s) 209, 265, 773, 796
RsaNI GTAC 4 cut(s) 208, 264, 772, 795
RseI CAYNNNNRTG 1 cut(s) 503
SacI GAGCTC 1 cut(s) 59
SaqAI TTAA 3 cut(s) 62, 237, 803
SatI GCNGC 1 cut(s) 401
Sau3AI GATC 2 cut(s) 346, 780
Sau96I GGNCC 1 cut(s) 296
ScaI AGTACT 1 cut(s) 773
SduI GDGCHC 1 cut(s) 59
SfaNI GCATC 2 cut(s) 664, 724
SfcI CTRYAG 1 cut(s) 652
Sfr274I CTCGAG 1 cut(s) 568
SlaI CTCGAG 1 cut(s) 568
SmiMI CAYNNNNRTG 1 cut(s) 503
SmlI CTYRAG 3 cut(s) 236, 291, 568
SmoI CTYRAG 3 cut(s) 236, 291, 568
Sse9I AATT 3 cut(s) 323, 404, 474
SspI AATATT 1 cut(s) 370
SspMI CTAG 2 cut(s) 711, 741
SstI GAGCTC 1 cut(s) 59
TaaI ACNGT 3 cut(s) 125, 268, 794
TaiI ACGT 2 cut(s) 21, 669
TaqI TCGA 3 cut(s) 315, 569, 672
TasI AATT 3 cut(s) 323, 404, 474
TatI WGTACW 1 cut(s) 771
TfiI GAWTC 3 cut(s) 189, 521, 701
Tru1I TTAA 3 cut(s) 62, 237, 803
Tru9I TTAA 3 cut(s) 62, 237, 803
TscAI CASTG 2 cut(s) 346, 531
TseFI GTSAC 2 cut(s) 119, 268
TseI GCWGC 1 cut(s) 400
Tsp45I GTSAC 2 cut(s) 119, 268
TspDTI ATGAA 2 cut(s) 324, 766
TspRI CASTG 2 cut(s) 346, 531
Tth111I GACNNNGTC 1 cut(s) 173
Van91I CCANNNNNTGG 1 cut(s) 456
Vha464I CTTAAG 1 cut(s) 236
XapI RAATTY 1 cut(s) 323
XceI RCATGY 1 cut(s) 232
XhoI CTCGAG 1 cut(s) 568
XmnI GAANNNNTTC 1 cut(s) 773
XspI CTAG 2 cut(s) 711, 741
ZrmI AGTACT 1 cut(s) 773
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.