FvH4_3g06510

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000406
Physical Location & Seq
Forward (+)
3733391 .. 3735399
2009 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g06510.t1

Sequence Viewer

Length: 921 bp
ATGGTGGCGCCCTACAATTGTGATAGAGTTGGCAAGATGTTGAAGATCGTCTTGTTCTTTCTGTCTGTCAGTTGTCCCTTCATGGCTGCAGCTTCAGCTGGTAATTTCTACCGGGACTTCGACCTAACTTGGGGTGATCATCGAGCTAAGATACTCGATGGTGGAAACCTCCTCACTCTTTCTCTCGACCGAATCTCTGGCTCTGGGTTTCAGTCCAAAAGAGAATATCTCTTCGGCAGAATCGACATGCAGCTCAAGCTTGTCGCCGGGAACTCCGCCGGCACCGTCACCGCTTACTATTTATCTTCACAAGGGCCGAATCACGATGAGATTGATTTCGAGTTCTTGGGGAACTTGAGTGGAGATCCTTATATTGTGCATACAAACGTTTACACGCAAGGGAAAGGGGATAGAGAGCAACAATTCCACCTATGGTTCGACCCAACAAAAAACTTTCATACATACTCAATCCTGTGGAATCCCCAACGTATCATATTCCTAGTGGACAACACACCCATAAGAGTGTTCAACAATGAGGAAGCAATTGGGGTTGGCTTCCCCAAGAAGCAACCCATGAAGCTCTACTCGAGTCTATGGAACGCGGACCAGTGGGCAACAAGAGGCGGGCTAATCAAAACCGACTGGTCCAAAGCTCCATTCACAGCATACTACAAAGGCTTCAATGCCAATGCCTGTGTTTGGTCTCGGGGTTCTTTGTCGTGTGCCAGTGGCCGAAGTACCCCTAGACCATATGGCTTGGGCATGAACTATAACAATGGATGGCAGACTCAAGGGCTTGATGCACCTGGCCGAAGAAGACTGAGATGGGTGCAGAGGTACCACATGATATATAACTATTGCACTGATCTGAAGCGATTCCCTCAGGGACGTCCAAGGGAGTGTAGGCGTTCTAGGGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_16 XET_C

Protein Analysis

307

Amino Acids

35.03

Weight (kDa)

9.57

Isoelectric Point (pI)

39.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 37 - 217 3.4e-67 Glycosyl hydrolases family 16
XET_C PF06955 258 - 301 1.3e-18 Xyloglucan endo-transglycosylase (XET) C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000406)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23730 AT4G14130
fragaria_vesca FvH4_3g06510 FvH4_4g12960 FvH4_6g38150 FvH4_6g38150 FvH4_6g38150 FvH4_6g38160 FvH4_6g38170 FvH4_6g38181 FvH4_6g38190
malus_domestica MD04G1020100.v1.1 MD09G1152400.v1.1 MD09G1152600.v1.1 MD09G1152700.v1.1 MD17G1139900.v1.1 MD17G1140000.v1.1
prunus_persica Prupe.1G169700_v2.0.a1 Prupe.3G171500_v2.0.a1 Prupe.3G171600_v2.0.a1 Prupe.3G171700_v2.0.a1 Prupe.3G171800_v2.0.a1 Prupe.3G172000_v2.0.a1 Prupe.3G172100_v2.0.a1 Prupe.4G072800_v2.0.a1
pyrus_communis pycom04g01620 pycom09g07160 pycom09g07220 pycom09g07230 pycom10g22480 pycom13g29190 pycom17g13320
rosa_chinensis RchiOBHm_Chr2g0151371 RchiOBHm_Chr2g0151381 RchiOBHm_Chr2g0151401 RchiOBHm_Chr2g0151421 RchiOBHm_Chr2g0151431 RchiOBHm_Chr2g0151441 RchiOBHm_Chr2g0152071 RchiOBHm_Chr4g0412651 RchiOBHm_Chr5g0012671
rosa_laevigata RLG00000008270 RLG00000020544 RLG00000020546 RLG00000020547 RLG00000020548 RLG00000020549 RLG00000020605 RLG00000031947
rosa_multiflora Rmu_co8167812.1_g000001 Rmu_co8363165.1_g000001 Rmu_co8513985.1_g000001 Rmu_sc0000940.1_g000002 Rmu_sc0000940.1_g000005 Rmu_sc0000940.1_g000006 Rmu_sc0000940.1_g000009 Rmu_sc0002516.1_g000015 Rmu_sc0002516.1_g000017 Rmu_sc0002516.1_g000018 Rmu_sc0043125.1_g000001
rosa_roxburghii Rroxscaffold_1G00063930 Rroxscaffold_2G00095990 Rroxscaffold_2G00096470 Rroxscaffold_2G00096480 Rroxscaffold_2G00096490 Rroxscaffold_2G00096500 Rroxscaffold_5G00356830
rosa_rugosa Rorug02G0425600 Rorug02G0425700 Rorug02G0425800 Rorug02G0425900 Rorug02G0430500 Rorug02G0430600 Rorug04G0115400 Rorug05G0003700
rosa_samantha Rh2BG499200 Rh2CG472700 Rh2CG472800 Rh2CG473000 Rh2CG473100 Rh2CG473300 Rh2CG477800 Rh2DG509800 Rh2DG509900 Rh2DG510100 Rh2DG510200 Rh2DG510300 Rh2DG515000 Rh4AG174700 Rh4BG174000 Rh5AG097500
rosa_wichuraiana Rw2G039850 Rw2G039860 Rw2G039870 Rw2G039880 Rw2G039890 Rw2G039900 Rw2G040400 Rw4G014650 Rw5G008540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 892
Acc65I GGTACC 1 cut(s) 837
AccB1I GGYRCC 3 cut(s) 7, 281, 837
AccII CGCG 1 cut(s) 602
AciI CCGC 4 cut(s) 276, 291, 602, 624
AclI AACGTT 1 cut(s) 387
AclWI GGATC 1 cut(s) 359
AcoI YGGCCR 2 cut(s) 730, 808
AcuI CTGAAG 2 cut(s) 78, 890
AcyI GRCGYC 2 cut(s) 8, 889
AfaI GTAC 2 cut(s) 739, 839
AfiI CCNNNNNNNGG 2 cut(s) 130, 699
AgsI TTSAA 3 cut(s) 43, 529, 682
AjnI CCWGG 1 cut(s) 805
AjuI GAANNNNNNNTTGG 2 cut(s) 641, 673
AluBI AGCT 7 cut(s) 92, 98, 146, 253, 259, 580, 653
AluI AGCT 7 cut(s) 92, 98, 146, 253, 259, 580, 653
Alw26I GTCTC 1 cut(s) 708
AlwI GGATC 1 cut(s) 359
Ama87I CYCGRG 2 cut(s) 586, 705
AoxI GGCC 3 cut(s) 314, 730, 808
ApeKI GCWGC 3 cut(s) 86, 89, 250
Asp700I GAANNNNTTC 1 cut(s) 875
Asp718I GGTACC 1 cut(s) 837
AspLEI GCGC 1 cut(s) 10
AspS9I GGNCC 3 cut(s) 314, 604, 645
AsuC2I CCSGG 2 cut(s) 113, 268
AsuHPI GGTGA 2 cut(s) 146, 280
AvaI CYCGRG 2 cut(s) 586, 705
AvaII GGWCC 2 cut(s) 604, 645
AxyI CCTNAGG 1 cut(s) 882
BanI GGYRCC 3 cut(s) 7, 281, 837
BbsI GAAGAC 1 cut(s) 823
BbvI GCAGC 3 cut(s) 73, 101, 262
BccI CCATC 3 cut(s) 152, 774, 819
BciT130I CCWGG 1 cut(s) 807
BclI TGATCA 1 cut(s) 136
BcnI CCSGG 2 cut(s) 113, 268
BcoDI GTCTC 1 cut(s) 708
BfaI CTAG 3 cut(s) 500, 744, 912
BfmI CTRYAG 1 cut(s) 87
BfoI RGCGCY 1 cut(s) 11
BisI GCNGC 3 cut(s) 87, 90, 251
BlsI GCNGC 3 cut(s) 88, 91, 252
Bme1390I CCNGG 3 cut(s) 113, 268, 807
Bme18I GGWCC 2 cut(s) 604, 645
BmeT110I CYCGRG 2 cut(s) 586, 705
BmgT120I GGNCC 3 cut(s) 314, 604, 645
BmiI GGNNCC 3 cut(s) 9, 283, 839
BmrFI CCNGG 3 cut(s) 113, 268, 807
BmsI GCATC 1 cut(s) 790
BpiI GAAGAC 1 cut(s) 823
BplI GAGNNNNNCTC 2 cut(s) 213, 245
BpuEI CTTGAG 3 cut(s) 239, 376, 774
BpuMI CCSGG 2 cut(s) 113, 268
BsaHI GRCGYC 2 cut(s) 8, 889
BsaI GGTCTC 1 cut(s) 708
BsaJI CCNNGG 1 cut(s) 893
Bsc4I CCNNNNNNNGG 2 cut(s) 130, 699
Bse118I RCCGGY 1 cut(s) 278
Bse1I ACTGG 3 cut(s) 607, 647, 726
Bse21I CCTNAGG 1 cut(s) 882
BseBI CCWGG 1 cut(s) 807
BseDI CCNNGG 1 cut(s) 893
BseGI GGATG 1 cut(s) 785
BseLI CCNNNNNNNGG 2 cut(s) 130, 699
BseMII CTCAG 2 cut(s) 812, 896
BseNI ACTGG 3 cut(s) 607, 647, 726
BseRI GAGGAG 1 cut(s) 161
BseXI GCAGC 3 cut(s) 73, 101, 262
BsgI GTGCAG 1 cut(s) 851
Bsh1236I CGCG 1 cut(s) 602
Bsh1285I CGRYCG 1 cut(s) 190
BshFI GGCC 3 cut(s) 316, 732, 810
BshNI GGYRCC 3 cut(s) 7, 281, 837
BsiEI CGRYCG 1 cut(s) 190
BsiHKCI CYCGRG 2 cut(s) 586, 705
BsiSI CCGG 3 cut(s) 112, 267, 279
BslFI GGGAC 3 cut(s) 60, 128, 900
BslI CCNNNNNNNGG 2 cut(s) 130, 699
BsmAI GTCTC 1 cut(s) 708
BsmFI GGGAC 3 cut(s) 60, 128, 900
BsnI GGCC 3 cut(s) 316, 732, 810
Bso31I GGTCTC 1 cut(s) 708
BsoBI CYCGRG 2 cut(s) 586, 705
Bsp143I GATC 4 cut(s) 45, 136, 364, 865
BspACI CCGC 4 cut(s) 276, 291, 602, 624
BspANI GGCC 3 cut(s) 316, 732, 810
BspCNI CTCAG 2 cut(s) 813, 895
BspFNI CGCG 1 cut(s) 602
BspLI GGNNCC 3 cut(s) 9, 283, 839
BspMAI CTGCAG 1 cut(s) 91
BspPI GGATC 1 cut(s) 359
BspT107I GGYRCC 3 cut(s) 7, 281, 837
BspTNI GGTCTC 1 cut(s) 708
BsrFI RCCGGY 1 cut(s) 278
BsrI ACTGG 3 cut(s) 607, 647, 726
BssAI RCCGGY 1 cut(s) 278
BssECI CCNNGG 1 cut(s) 893
BssMI GATC 4 cut(s) 45, 136, 364, 865
BssNI GRCGYC 2 cut(s) 8, 889
BssT1I CCWWGG 1 cut(s) 893
Bst2UI CCWGG 1 cut(s) 807
Bst4CI ACNGT 1 cut(s) 286
Bst6I CTCTTC 1 cut(s) 236
BstACI GRCGYC 2 cut(s) 8, 889
BstC8I GCNNGC 2 cut(s) 280, 626
BstDEI CTNAG 3 cut(s) 147, 821, 882
BstF5I GGATG 1 cut(s) 785
BstFNI CGCG 1 cut(s) 602
BstH2I RGCGCY 1 cut(s) 11
BstHHI GCGC 1 cut(s) 10
BstKTI GATC 4 cut(s) 48, 139, 367, 868
BstMAI GTCTC 1 cut(s) 708
BstMBI GATC 4 cut(s) 45, 136, 364, 865
BstMCI CGRYCG 1 cut(s) 190
BstMWI GCNNNNNNNGC 2 cut(s) 95, 256
BstNI CCWGG 1 cut(s) 807
BstNSI RCATGY 1 cut(s) 250
BstSCI CCNGG 3 cut(s) 111, 266, 805
BstSFI CTRYAG 1 cut(s) 87
BstUI CGCG 1 cut(s) 602
BstV1I GCAGC 3 cut(s) 73, 101, 262
BstV2I GAAGAC 1 cut(s) 823
BstX2I RGATCY 1 cut(s) 364
BstYI RGATCY 1 cut(s) 364
Bsu36I CCTNAGG 1 cut(s) 882
BsuRI GGCC 3 cut(s) 316, 732, 810
BtsCI GGATG 1 cut(s) 785
BtsIMutI CAGTG 3 cut(s) 614, 733, 861
Cac8I GCNNGC 2 cut(s) 280, 626
CfoI GCGC 1 cut(s) 10
Cfr10I RCCGGY 1 cut(s) 278
Cfr13I GGNCC 3 cut(s) 314, 604, 645
Csp6I GTAC 2 cut(s) 738, 838
CviAII CATG 5 cut(s) 82, 247, 574, 763, 844
CviQI GTAC 2 cut(s) 738, 838
DdeI CTNAG 3 cut(s) 147, 821, 882
DinI GGCGCC 1 cut(s) 9
DpnI GATC 4 cut(s) 47, 138, 366, 867
DpnII GATC 4 cut(s) 45, 136, 364, 865
EaeI YGGCCR 2 cut(s) 730, 808
Eam1104I CTCTTC 1 cut(s) 236
EarI CTCTTC 1 cut(s) 236
EciI GGCGGA 1 cut(s) 265
Eco130I CCWWGG 1 cut(s) 893
Eco31I GGTCTC 1 cut(s) 708
Eco47I GGWCC 2 cut(s) 604, 645
Eco57I CTGAAG 2 cut(s) 78, 890
Eco81I CCTNAGG 1 cut(s) 882
Eco88I CYCGRG 2 cut(s) 586, 705
EcoRII CCWGG 1 cut(s) 805
EcoT14I CCWWGG 1 cut(s) 893
EgeI GGCGCC 1 cut(s) 9
EheI GGCGCC 1 cut(s) 9
ErhI CCWWGG 1 cut(s) 893
FaeI CATG 5 cut(s) 85, 250, 577, 766, 847
FalI AAGNNNNNCTT 2 cut(s) 35, 67
FaqI GGGAC 3 cut(s) 60, 128, 900
FatI CATG 5 cut(s) 81, 246, 573, 762, 843
FauI CCCGC 1 cut(s) 617
FauNDI CATATG 1 cut(s) 751
FbaI TGATCA 1 cut(s) 136
Fnu4HI GCNGC 3 cut(s) 87, 90, 251
FokI GGATG 1 cut(s) 792
Fsp4HI GCNGC 3 cut(s) 87, 90, 251
FspBI CTAG 3 cut(s) 500, 744, 912
GlaI GCGC 1 cut(s) 9
GluI GCNGC 3 cut(s) 87, 90, 251
HaeII RGCGCY 1 cut(s) 11
HaeIII GGCC 3 cut(s) 316, 732, 810
HapII CCGG 3 cut(s) 112, 267, 279
HhaI GCGC 1 cut(s) 10
Hin1I GRCGYC 2 cut(s) 8, 889
Hin1II CATG 5 cut(s) 85, 250, 577, 766, 847
Hin6I GCGC 1 cut(s) 8
HinP1I GCGC 1 cut(s) 8
HindIII AAGCTT 1 cut(s) 257
HinfI GANTC 7 cut(s) 192, 240, 319, 478, 589, 787, 876
HpaII CCGG 3 cut(s) 112, 267, 279
HphI GGTGA 2 cut(s) 146, 280
Hpy166II GTNNAC 2 cut(s) 391, 505
Hpy188I TCNGA 1 cut(s) 870
Hpy188III TCNNGA 2 cut(s) 185, 323
Hpy8I GTNNAC 2 cut(s) 391, 505
HpyAV CCTTC 1 cut(s) 88
HpyCH4III ACNGT 1 cut(s) 286
HpyCH4IV ACGT 3 cut(s) 387, 487, 889
HpyCH4V TGCA 6 cut(s) 89, 250, 379, 803, 832, 861
HpyF10VI GCNNNNNNNGC 2 cut(s) 95, 256
HpyF3I CTNAG 3 cut(s) 147, 821, 882
HpySE526I ACGT 3 cut(s) 387, 487, 889
Hsp92I GRCGYC 2 cut(s) 8, 889
Hsp92II CATG 5 cut(s) 85, 250, 577, 766, 847
HspAI GCGC 1 cut(s) 8
KasI GGCGCC 1 cut(s) 7
KpnI GGTACC 1 cut(s) 841
KroI GCCGGC 1 cut(s) 278
KroNI GCCGGC 1 cut(s) 280
Ksp22I TGATCA 1 cut(s) 136
Kzo9I GATC 4 cut(s) 45, 136, 364, 865
LmnI GCTCC 1 cut(s) 658
Lsp1109I GCAGC 3 cut(s) 73, 101, 262
LweI GCATC 1 cut(s) 790
MaeI CTAG 3 cut(s) 500, 744, 912
MaeII ACGT 3 cut(s) 387, 487, 889
MaeIII GTNAC 1 cut(s) 286
MalI GATC 4 cut(s) 47, 138, 366, 867
MboI GATC 4 cut(s) 45, 136, 364, 865
MboII GAAGA 5 cut(s) 55, 223, 297, 825, 828
MfeI CAATTG 2 cut(s) 16, 543
MflI RGATCY 1 cut(s) 364
MluCI AATT 4 cut(s) 16, 103, 422, 543
Mly113I GGCGCC 1 cut(s) 8
MlyI GAGTC 2 cut(s) 598, 781
MnlI CCTC 6 cut(s) 179, 182, 529, 614, 828, 891
MroNI GCCGGC 1 cut(s) 278
MroXI GAANNNNTTC 1 cut(s) 875
MslI CAYNNNNRTG 1 cut(s) 521
MspA1I CMGCKG 1 cut(s) 98
MspI CCGG 3 cut(s) 112, 267, 279
MspR9I CCNGG 3 cut(s) 113, 268, 807
MunI CAATTG 2 cut(s) 16, 543
MvaI CCWGG 1 cut(s) 807
MvnI CGCG 1 cut(s) 602
MwoI GCNNNNNNNGC 2 cut(s) 95, 256
NaeI GCCGGC 1 cut(s) 280
NarI GGCGCC 1 cut(s) 8
NciI CCSGG 2 cut(s) 113, 268
NdeI CATATG 1 cut(s) 751
NdeII GATC 4 cut(s) 45, 136, 364, 865
NgoMIV GCCGGC 1 cut(s) 278
NlaIII CATG 5 cut(s) 85, 250, 577, 766, 847
NlaIV GGNNCC 3 cut(s) 9, 283, 839
NmuCI GTSAC 1 cut(s) 286
NspI RCATGY 1 cut(s) 250
PaeR7I CTCGAG 1 cut(s) 586
PcsI WCGNNNNNNNCGW 1 cut(s) 240
PdiI GCCGGC 1 cut(s) 280
PdmI GAANNNNTTC 1 cut(s) 875
PfeI GAWTC 5 cut(s) 192, 240, 319, 478, 876
PkrI GCNGC 3 cut(s) 88, 91, 252
PleI GAGTC 2 cut(s) 597, 781
PluTI GGCGCC 1 cut(s) 11
PpsI GAGTC 2 cut(s) 597, 781
Psp1406I AACGTT 1 cut(s) 387
Psp6I CCWGG 1 cut(s) 805
PspGI CCWGG 1 cut(s) 805
PspN4I GGNNCC 3 cut(s) 9, 283, 839
PspPI GGNCC 3 cut(s) 314, 604, 645
PspXI VCTCGAGB 1 cut(s) 586
PstI CTGCAG 1 cut(s) 91
PsuI RGATCY 1 cut(s) 364
PvuII CAGCTG 1 cut(s) 98
RsaI GTAC 2 cut(s) 739, 839
RsaNI GTAC 2 cut(s) 738, 838
RseI CAYNNNNRTG 1 cut(s) 521
SatI GCNGC 3 cut(s) 87, 90, 251
Sau3AI GATC 4 cut(s) 45, 136, 364, 865
Sau96I GGNCC 3 cut(s) 314, 604, 645
SchI GAGTC 2 cut(s) 598, 781
ScrFI CCNGG 3 cut(s) 113, 268, 807
SfaNI GCATC 1 cut(s) 790
SfcI CTRYAG 1 cut(s) 87
SfoI GGCGCC 1 cut(s) 9
Sfr274I CTCGAG 1 cut(s) 586
SinI GGWCC 2 cut(s) 604, 645
SlaI CTCGAG 1 cut(s) 586
SmiMI CAYNNNNRTG 1 cut(s) 521
SmlI CTYRAG 4 cut(s) 254, 355, 586, 789
SmoI CTYRAG 4 cut(s) 254, 355, 586, 789
Sse9I AATT 4 cut(s) 16, 103, 422, 543
SsiI CCGC 4 cut(s) 276, 291, 602, 624
SspDI GGCGCC 1 cut(s) 7
SspMI CTAG 3 cut(s) 500, 744, 912
StyD4I CCNGG 3 cut(s) 111, 266, 805
StyI CCWWGG 1 cut(s) 893
TaaI ACNGT 1 cut(s) 286
TaiI ACGT 3 cut(s) 390, 490, 892
TaqI TCGA 8 cut(s) 120, 142, 156, 186, 243, 339, 438, 587
TaqII GACCGA 1 cut(s) 204
TasI AATT 4 cut(s) 16, 103, 422, 543
TfiI GAWTC 5 cut(s) 192, 240, 319, 478, 876
TscAI CASTG 3 cut(s) 614, 733, 868
TseFI GTSAC 1 cut(s) 286
TseI GCWGC 3 cut(s) 86, 89, 250
Tsp45I GTSAC 1 cut(s) 286
TspDTI ATGAA 4 cut(s) 70, 446, 590, 779
TspRI CASTG 3 cut(s) 614, 733, 868
VpaK11BI GGWCC 2 cut(s) 604, 645
XceI RCATGY 1 cut(s) 250
XhoI CTCGAG 1 cut(s) 586
XmnI GAANNNNTTC 1 cut(s) 875
XspI CTAG 3 cut(s) 500, 744, 912
ZraI GACGTC 1 cut(s) 890
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.