FvH4_4g12960

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000406
Physical Location & Seq
Forward (+)
16640418 .. 16642663
2246 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g12960.t1

Sequence Viewer

Length: 846 bp
ATGGGGATTTCTGGTAATGGGGCTTGGTTGGTCTTGTTGATTGGTTTAGCATTGAGCTCTTTCATAGTAGGCTCTTATGCTGGCAACTTCTACCAAGACTTTGACTTGACATGGGGTGGTAACCGTCCTAAGATATTCAAGGGAGGTCAGCTTCTTTCTTTGTCTTTGGACAAGGTTTCTGGCTCTGGATTCCAGTCCAAGAAAGAGTACCTCTTTGGGAGGATTGACATGCAACTTAAGCTTGTTGCCGGAAACTCTGCCGGCACTGTCACTGCCTACTACCTGTCTTCTCAAGGCCCTACACATGATGAAATCGACTTTGAATTCTTGGGAAATGTCAGTGGGGATCCATATGTGTTGCACACCAATGTTTTCACCCAGGGCAAGGGAAACAGAGAGCAGCAATTCTATCTCTGGTTTGACCCCTCAAAGAACTTCCACACTTACTCCATCATCTGGAAGCCCCAACACATAATTTTCTTGGTGGACAACACTCCCATTAGAGTGTTCAAGAATGCTGAATCGCTTGGTGTTCCATTTCCTAAGAACCAACCAATGAGGATTTACTCAAGCCTTTGGAATGCTGATGATTGGGCTACAAGAGGAGGATTAGTGAAAACAGATTGGTCAAAGGCACCCTTTACAGCATACTACAGGAACTTCAATGTCATCGATGCTAAATCATCCAAATCATTCTCTGATTCTCTAGCTAGTTGGCAGACCAATGCACTTGATGCTCCTGGCCGAAGACGCCTGAGATGGGTTCAGAAGTACTTCATGATCTACAACTACTGCTCCGATTTCAAACGCTTCCCACAAGGTTTTCCTGCCGAGTGTAGAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_16 XET_C

Protein Analysis

282

Amino Acids

31.92

Weight (kDa)

9.34

Isoelectric Point (pI)

31.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 31 - 211 1.3e-67 Glycosyl hydrolases family 16
XET_C PF06955 238 - 279 2.7e-18 Xyloglucan endo-transglycosylase (XET) C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000406)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23730 AT4G14130
fragaria_vesca FvH4_3g06510 FvH4_4g12960 FvH4_6g38150 FvH4_6g38150 FvH4_6g38150 FvH4_6g38160 FvH4_6g38170 FvH4_6g38181 FvH4_6g38190
malus_domestica MD04G1020100.v1.1 MD09G1152400.v1.1 MD09G1152600.v1.1 MD09G1152700.v1.1 MD17G1139900.v1.1 MD17G1140000.v1.1
prunus_persica Prupe.1G169700_v2.0.a1 Prupe.3G171500_v2.0.a1 Prupe.3G171600_v2.0.a1 Prupe.3G171700_v2.0.a1 Prupe.3G171800_v2.0.a1 Prupe.3G172000_v2.0.a1 Prupe.3G172100_v2.0.a1 Prupe.4G072800_v2.0.a1
pyrus_communis pycom04g01620 pycom09g07160 pycom09g07220 pycom09g07230 pycom10g22480 pycom13g29190 pycom17g13320
rosa_chinensis RchiOBHm_Chr2g0151371 RchiOBHm_Chr2g0151381 RchiOBHm_Chr2g0151401 RchiOBHm_Chr2g0151421 RchiOBHm_Chr2g0151431 RchiOBHm_Chr2g0151441 RchiOBHm_Chr2g0152071 RchiOBHm_Chr4g0412651 RchiOBHm_Chr5g0012671
rosa_laevigata RLG00000008270 RLG00000020544 RLG00000020546 RLG00000020547 RLG00000020548 RLG00000020549 RLG00000020605 RLG00000031947
rosa_multiflora Rmu_co8167812.1_g000001 Rmu_co8363165.1_g000001 Rmu_co8513985.1_g000001 Rmu_sc0000940.1_g000002 Rmu_sc0000940.1_g000005 Rmu_sc0000940.1_g000006 Rmu_sc0000940.1_g000009 Rmu_sc0002516.1_g000015 Rmu_sc0002516.1_g000017 Rmu_sc0002516.1_g000018 Rmu_sc0043125.1_g000001
rosa_roxburghii Rroxscaffold_1G00063930 Rroxscaffold_2G00095990 Rroxscaffold_2G00096470 Rroxscaffold_2G00096480 Rroxscaffold_2G00096490 Rroxscaffold_2G00096500 Rroxscaffold_5G00356830
rosa_rugosa Rorug02G0425600 Rorug02G0425700 Rorug02G0425800 Rorug02G0425900 Rorug02G0430500 Rorug02G0430600 Rorug04G0115400 Rorug05G0003700
rosa_samantha Rh2BG499200 Rh2CG472700 Rh2CG472800 Rh2CG473000 Rh2CG473100 Rh2CG473300 Rh2CG477800 Rh2DG509800 Rh2DG509900 Rh2DG510100 Rh2DG510200 Rh2DG510300 Rh2DG515000 Rh4AG174700 Rh4BG174000 Rh5AG097500
rosa_wichuraiana Rw2G039850 Rw2G039860 Rw2G039870 Rw2G039880 Rw2G039890 Rw2G039900 Rw2G040400 Rw4G014650 Rw5G008540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 634
AccB7I CCANNNNNTGG 1 cut(s) 456
AclWI GGATC 2 cut(s) 341, 354
AcoI YGGCCR 1 cut(s) 742
AcsI RAATTY 1 cut(s) 323
AcyI GRCGYC 1 cut(s) 751
AfaI GTAC 2 cut(s) 209, 773
AfiI CCNNNNNNNGG 3 cut(s) 385, 456, 760
AflII CTTAAG 1 cut(s) 236
AgsI TTSAA 5 cut(s) 139, 323, 511, 664, 805
AjnI CCWGG 2 cut(s) 378, 739
AluBI AGCT 4 cut(s) 57, 151, 241, 710
AluI AGCT 4 cut(s) 57, 151, 241, 710
Alw21I GWGCWC 1 cut(s) 59
AlwI GGATC 2 cut(s) 341, 354
AoxI GGCC 2 cut(s) 295, 742
ApeKI GCWGC 1 cut(s) 400
ApoI RAATTY 1 cut(s) 323
Asp700I GAANNNNTTC 1 cut(s) 773
AspS9I GGNCC 1 cut(s) 296
AsuHPI GGTGA 1 cut(s) 367
BamHI GGATCC 1 cut(s) 346
BanI GGYRCC 1 cut(s) 634
BanII GRGCYC 1 cut(s) 59
BbsI GAAGAC 2 cut(s) 279, 754
Bbv12I GWGCWC 1 cut(s) 59
BbvI GCAGC 1 cut(s) 412
BccI CCATC 2 cut(s) 458, 753
BciT130I CCWGG 2 cut(s) 380, 741
BfaI CTAG 2 cut(s) 707, 711
BfmI CTRYAG 1 cut(s) 652
BfrI CTTAAG 1 cut(s) 236
BisI GCNGC 1 cut(s) 401
BlsI GCNGC 1 cut(s) 402
BmcAI AGTACT 1 cut(s) 773
Bme1390I CCNGG 2 cut(s) 380, 741
BmgT120I GGNCC 1 cut(s) 296
BmiI GGNNCC 2 cut(s) 348, 636
BmrFI CCNGG 2 cut(s) 380, 741
BmsI GCATC 2 cut(s) 664, 724
BpiI GAAGAC 2 cut(s) 279, 754
BpuEI CTTGAG 2 cut(s) 276, 553
Bsa29I ATCGAT 1 cut(s) 672
BsaHI GRCGYC 1 cut(s) 751
BsaJI CCNNGG 2 cut(s) 378, 379
BsaXI ACNNNNNCTCC 6 cut(s) 431, 461, 597, 627, 779, 809
Bsc4I CCNNNNNNNGG 3 cut(s) 385, 456, 760
Bse118I RCCGGY 1 cut(s) 260
Bse1I ACTGG 1 cut(s) 193
BseBI CCWGG 2 cut(s) 380, 741
BseCI ATCGAT 1 cut(s) 672
BseDI CCNNGG 2 cut(s) 378, 379
BseGI GGATG 1 cut(s) 683
BseLI CCNNNNNNNGG 3 cut(s) 385, 456, 760
BseMII CTCAG 1 cut(s) 746
BseNI ACTGG 1 cut(s) 193
BseRI GAGGAG 1 cut(s) 618
BseXI GCAGC 1 cut(s) 412
BshFI GGCC 2 cut(s) 297, 744
BshNI GGYRCC 1 cut(s) 634
BshVI ATCGAT 1 cut(s) 672
BsiHKAI GWGCWC 1 cut(s) 59
BsiSI CCGG 2 cut(s) 249, 261
BslI CCNNNNNNNGG 3 cut(s) 385, 456, 760
BsmI GAATGC 2 cut(s) 520, 586
BsnI GGCC 2 cut(s) 297, 744
Bsp1286I GDGCHC 1 cut(s) 59
Bsp143I GATC 2 cut(s) 346, 780
BspANI GGCC 2 cut(s) 297, 744
BspCNI CTCAG 1 cut(s) 747
BspDI ATCGAT 1 cut(s) 672
BspHI TCATGA 1 cut(s) 777
BspLI GGNNCC 2 cut(s) 348, 636
BspPI GGATC 2 cut(s) 341, 354
BspT107I GGYRCC 1 cut(s) 634
BspTI CTTAAG 1 cut(s) 236
BsrFI RCCGGY 1 cut(s) 260
BsrI ACTGG 1 cut(s) 193
BssAI RCCGGY 1 cut(s) 260
BssECI CCNNGG 2 cut(s) 378, 379
BssMI GATC 2 cut(s) 346, 780
BssNI GRCGYC 1 cut(s) 751
Bst2UI CCWGG 2 cut(s) 380, 741
Bst4CI ACNGT 2 cut(s) 125, 268
BstACI GRCGYC 1 cut(s) 751
BstAFI CTTAAG 1 cut(s) 236
BstAPI GCANNNNNTGC 1 cut(s) 734
BstC8I GCNNGC 2 cut(s) 82, 262
BstDEI CTNAG 3 cut(s) 129, 543, 755
BstEII GGTNACC 1 cut(s) 119
BstF5I GGATG 1 cut(s) 683
BstKTI GATC 2 cut(s) 349, 783
BstMBI GATC 2 cut(s) 346, 780
BstMWI GCNNNNNNNGC 3 cut(s) 238, 734, 750
BstNI CCWGG 2 cut(s) 380, 741
BstNSI RCATGY 1 cut(s) 232
BstPI GGTNACC 1 cut(s) 119
BstSCI CCNGG 2 cut(s) 378, 739
BstSFI CTRYAG 1 cut(s) 652
BstV1I GCAGC 1 cut(s) 412
BstV2I GAAGAC 2 cut(s) 279, 754
BstX2I RGATCY 1 cut(s) 346
BstYI RGATCY 1 cut(s) 346
Bsu15I ATCGAT 1 cut(s) 672
BsuRI GGCC 2 cut(s) 297, 744
BsuTUI ATCGAT 1 cut(s) 672
BtsCI GGATG 1 cut(s) 683
BtsI GCAGTG 1 cut(s) 270
BtsIMutI CAGTG 3 cut(s) 264, 270, 346
Cac8I GCNNGC 2 cut(s) 82, 262
CciI TCATGA 1 cut(s) 777
Cfr10I RCCGGY 1 cut(s) 260
Cfr13I GGNCC 1 cut(s) 296
ClaI ATCGAT 1 cut(s) 672
CseI GACGC 1 cut(s) 759
Csp6I GTAC 2 cut(s) 208, 772
CviAII CATG 4 cut(s) 111, 229, 305, 778
CviQI GTAC 2 cut(s) 208, 772
DdeI CTNAG 3 cut(s) 129, 543, 755
DpnI GATC 2 cut(s) 348, 782
DpnII GATC 2 cut(s) 346, 780
EaeI YGGCCR 1 cut(s) 742
Ecl136II GAGCTC 1 cut(s) 57
Eco24I GRGCYC 1 cut(s) 59
Eco53kI GAGCTC 1 cut(s) 57
Eco91I GGTNACC 1 cut(s) 119
EcoICRI GAGCTC 1 cut(s) 57
EcoO109I RGGNCCY 1 cut(s) 296
EcoO65I GGTNACC 1 cut(s) 119
EcoRI GAATTC 1 cut(s) 323
EcoRII CCWGG 2 cut(s) 378, 739
EcoT38I GRGCYC 1 cut(s) 59
FaeI CATG 4 cut(s) 114, 232, 308, 781
FalI AAGNNNNNCTT 2 cut(s) 623, 655
FatI CATG 4 cut(s) 110, 228, 304, 777
FauNDI CATATG 1 cut(s) 352
Fnu4HI GCNGC 1 cut(s) 401
FokI GGATG 1 cut(s) 670
FriOI GRGCYC 1 cut(s) 59
Fsp4HI GCNGC 1 cut(s) 401
FspBI CTAG 2 cut(s) 707, 711
GluI GCNGC 1 cut(s) 401
HaeIII GGCC 2 cut(s) 297, 744
HapII CCGG 2 cut(s) 249, 261
HgaI GACGC 1 cut(s) 759
Hin1I GRCGYC 1 cut(s) 751
Hin1II CATG 4 cut(s) 114, 232, 308, 781
HindIII AAGCTT 1 cut(s) 239
HinfI GANTC 3 cut(s) 189, 521, 701
HpaII CCGG 2 cut(s) 249, 261
HphI GGTGA 1 cut(s) 367
Hpy166II GTNNAC 1 cut(s) 487
Hpy188I TCNGA 3 cut(s) 700, 768, 799
Hpy188III TCNNGA 4 cut(s) 186, 457, 511, 778
Hpy8I GTNNAC 1 cut(s) 487
HpyCH4III ACNGT 2 cut(s) 125, 268
HpyCH4V TGCA 3 cut(s) 232, 361, 728
HpyF10VI GCNNNNNNNGC 3 cut(s) 238, 734, 750
HpyF3I CTNAG 3 cut(s) 129, 543, 755
Hsp92I GRCGYC 1 cut(s) 751
Hsp92II CATG 4 cut(s) 114, 232, 308, 781
KroI GCCGGC 1 cut(s) 260
KroNI GCCGGC 1 cut(s) 262
Kzo9I GATC 2 cut(s) 346, 780
LmnI GCTCC 2 cut(s) 742, 800
Lsp1109I GCAGC 1 cut(s) 412
LweI GCATC 2 cut(s) 664, 724
MaeI CTAG 2 cut(s) 707, 711
MaeIII GTNAC 2 cut(s) 119, 268
MalI GATC 2 cut(s) 348, 782
MboI GATC 2 cut(s) 346, 780
MboII GAAGA 2 cut(s) 279, 759
MflI RGATCY 1 cut(s) 346
MhlI GDGCHC 1 cut(s) 59
MluCI AATT 3 cut(s) 323, 404, 474
MnlI CCTC 7 cut(s) 137, 213, 221, 436, 552, 596, 599
MroNI GCCGGC 1 cut(s) 260
MroXI GAANNNNTTC 1 cut(s) 773
MseI TTAA 1 cut(s) 237
MslI CAYNNNNRTG 2 cut(s) 366, 503
MspCI CTTAAG 1 cut(s) 236
MspI CCGG 2 cut(s) 249, 261
MspR9I CCNGG 2 cut(s) 380, 741
Mva1269I GAATGC 2 cut(s) 520, 586
MvaI CCWGG 2 cut(s) 380, 741
MwoI GCNNNNNNNGC 3 cut(s) 238, 734, 750
NaeI GCCGGC 1 cut(s) 262
NdeI CATATG 1 cut(s) 352
NdeII GATC 2 cut(s) 346, 780
NgoMIV GCCGGC 1 cut(s) 260
NlaIII CATG 4 cut(s) 114, 232, 308, 781
NlaIV GGNNCC 2 cut(s) 348, 636
NmuCI GTSAC 1 cut(s) 268
NspI RCATGY 1 cut(s) 232
PagI TCATGA 1 cut(s) 777
PasI CCCWGGG 1 cut(s) 379
PctI GAATGC 2 cut(s) 520, 586
PdiI GCCGGC 1 cut(s) 262
PdmI GAANNNNTTC 1 cut(s) 773
PfeI GAWTC 3 cut(s) 189, 521, 701
PflMI CCANNNNNTGG 1 cut(s) 456
PkrI GCNGC 1 cut(s) 402
Psp124BI GAGCTC 1 cut(s) 59
Psp6I CCWGG 2 cut(s) 378, 739
PspEI GGTNACC 1 cut(s) 119
PspGI CCWGG 2 cut(s) 378, 739
PspN4I GGNNCC 2 cut(s) 348, 636
PspPI GGNCC 1 cut(s) 296
PsuI RGATCY 1 cut(s) 346
RsaI GTAC 2 cut(s) 209, 773
RsaNI GTAC 2 cut(s) 208, 772
RseI CAYNNNNRTG 2 cut(s) 366, 503
SacI GAGCTC 1 cut(s) 59
SaqAI TTAA 1 cut(s) 237
SatI GCNGC 1 cut(s) 401
Sau3AI GATC 2 cut(s) 346, 780
Sau96I GGNCC 1 cut(s) 296
ScaI AGTACT 1 cut(s) 773
ScrFI CCNGG 2 cut(s) 380, 741
SduI GDGCHC 1 cut(s) 59
SetI ASST 9 cut(s) 59, 148, 153, 177, 213, 243, 285, 712, 823
SfaNI GCATC 2 cut(s) 664, 724
SfcI CTRYAG 1 cut(s) 652
SmiMI CAYNNNNRTG 2 cut(s) 366, 503
SmlI CTYRAG 3 cut(s) 236, 291, 568
SmoI CTYRAG 3 cut(s) 236, 291, 568
Sse9I AATT 3 cut(s) 323, 404, 474
SspMI CTAG 2 cut(s) 707, 711
SstI GAGCTC 1 cut(s) 59
StyD4I CCNGG 2 cut(s) 378, 739
TaaI ACNGT 2 cut(s) 125, 268
TaqI TCGA 2 cut(s) 315, 672
TasI AATT 3 cut(s) 323, 404, 474
TatI WGTACW 1 cut(s) 771
TfiI GAWTC 3 cut(s) 189, 521, 701
Tru1I TTAA 1 cut(s) 237
Tru9I TTAA 1 cut(s) 237
TscAI CASTG 3 cut(s) 271, 277, 346
TseFI GTSAC 1 cut(s) 268
TseI GCWGC 1 cut(s) 400
Tsp45I GTSAC 1 cut(s) 268
TspDTI ATGAA 3 cut(s) 52, 324, 766
TspRI CASTG 3 cut(s) 271, 277, 346
Van91I CCANNNNNTGG 1 cut(s) 456
Vha464I CTTAAG 1 cut(s) 236
XapI RAATTY 1 cut(s) 323
XceI RCATGY 1 cut(s) 232
XmnI GAANNNNTTC 1 cut(s) 773
XspI CTAG 2 cut(s) 707, 711
ZrmI AGTACT 1 cut(s) 773
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.