Rh2DG510300

xyloglucan:xyloglucosyl transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000406
Physical Location & Seq
Forward (+)
73442864 .. 73446012
3149 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG510300.1

Sequence Viewer

Length: 945 bp
ATGTCTTCTTGTAAGCTCTCAATAACGGTGCTTTTTCTCACGTTGTTCATGATGGCCGGCATAGCTGCCTCAGCTAATTTCTGGGAAGACTTTGAACAAACATACGGCGACCAGCGTCTTCAATTACTTGATGGAGGGAAACATTTCACGCTCAGCCAGGACGCGAATTCTGGGGCGGGATTCAAATCCAAGAACGAATACCTATTTGGACGTTTCGACATGGATATGAAGCTCGCGCCTGGGAACTCAGCTGGTACTGTCACCACATTTTATTTATCTTCTCCTCAAGGGCACGAACACGATGAGATAGACATAGAGTTTTTGGGCAACTCTTCTGGGAGTCCGTACACTCTCCATACCAATGTGTTCAGTAAGGGTAAAGGAAATAAAGAACAACAATTTCATCTTTGGTTTGATCCCACACAAGATTTTCACAACTATACAATTATCTGGAACCCCCATCGTATTATATGGATGGTGGATAACAGTCCCATCAGGATTTTCAACAACGTCGAAGCATCAATTGATGTTCCATATCCTCATAGCAAACCGATGAGGATTTACTGCAGCTTCTGGAATGCCGATGACTGGGCTACACAAGGTGGAAAAGTAAAGGCCGACTGGTCTCAAGGTTCTAAAACAGTCTCTTACAAAGACCACAAGATCAATGCTTGTCTTCCTTGGGATCAGGGCTGTGCTTCGAACACAGCCGGCAATTCATGGCAGAATATTGATCTTGGTCGTTTAGGTCATGATAGGCTTCGATGGGTACAAGAGAAGTACAGGATCAGCAACTACTGTGACCCCAGTAAATCTCCCCAAGGTGTCCCCCCACGCGAGTGTATGCACTCCGAGTTTCAGAGCCCCCCAAATCCCAACCCTGCAACTCCCCGCGCTCCCTACATTCCCGACCTTAAAAATCCCGGCAACAAACCCAAATACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Glyco_hydro_16 XET_C

Protein Analysis

314

Amino Acids

35.6

Weight (kDa)

6.59

Isoelectric Point (pI)

43.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 29 - 209 2e-63 Glycosyl hydrolases family 16
XET_C PF06955 238 - 281 5.5e-06 Xyloglucan endo-transglycosylase (XET) C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000406)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23730 AT4G14130
fragaria_vesca FvH4_3g06510 FvH4_4g12960 FvH4_6g38150 FvH4_6g38150 FvH4_6g38150 FvH4_6g38160 FvH4_6g38170 FvH4_6g38181 FvH4_6g38190
malus_domestica MD04G1020100.v1.1 MD09G1152400.v1.1 MD09G1152600.v1.1 MD09G1152700.v1.1 MD17G1139900.v1.1 MD17G1140000.v1.1
prunus_persica Prupe.1G169700_v2.0.a1 Prupe.3G171500_v2.0.a1 Prupe.3G171600_v2.0.a1 Prupe.3G171700_v2.0.a1 Prupe.3G171800_v2.0.a1 Prupe.3G172000_v2.0.a1 Prupe.3G172100_v2.0.a1 Prupe.4G072800_v2.0.a1
pyrus_communis pycom04g01620 pycom09g07160 pycom09g07220 pycom09g07230 pycom10g22480 pycom13g29190 pycom17g13320
rosa_chinensis RchiOBHm_Chr2g0151371 RchiOBHm_Chr2g0151381 RchiOBHm_Chr2g0151401 RchiOBHm_Chr2g0151421 RchiOBHm_Chr2g0151431 RchiOBHm_Chr2g0151441 RchiOBHm_Chr2g0152071 RchiOBHm_Chr4g0412651 RchiOBHm_Chr5g0012671
rosa_laevigata RLG00000008270 RLG00000020544 RLG00000020546 RLG00000020547 RLG00000020548 RLG00000020549 RLG00000020605 RLG00000031947
rosa_multiflora Rmu_co8167812.1_g000001 Rmu_co8363165.1_g000001 Rmu_co8513985.1_g000001 Rmu_sc0000940.1_g000002 Rmu_sc0000940.1_g000005 Rmu_sc0000940.1_g000006 Rmu_sc0000940.1_g000009 Rmu_sc0002516.1_g000015 Rmu_sc0002516.1_g000017 Rmu_sc0002516.1_g000018 Rmu_sc0043125.1_g000001
rosa_roxburghii Rroxscaffold_1G00063930 Rroxscaffold_2G00095990 Rroxscaffold_2G00096470 Rroxscaffold_2G00096480 Rroxscaffold_2G00096490 Rroxscaffold_2G00096500 Rroxscaffold_5G00356830
rosa_rugosa Rorug02G0425600 Rorug02G0425700 Rorug02G0425800 Rorug02G0425900 Rorug02G0430500 Rorug02G0430600 Rorug04G0115400 Rorug05G0003700
rosa_samantha Rh2BG499200 Rh2CG472700 Rh2CG472800 Rh2CG473000 Rh2CG473100 Rh2CG473300 Rh2CG477800 Rh2DG509800 Rh2DG509900 Rh2DG510100 Rh2DG510200 Rh2DG510300 Rh2DG515000 Rh4AG174700 Rh4BG174000 Rh5AG097500
rosa_wichuraiana Rw2G039850 Rw2G039860 Rw2G039870 Rw2G039880 Rw2G039890 Rw2G039900 Rw2G040400 Rw4G014650 Rw5G008540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 4 cut(s) 164, 236, 837, 894
AciI CCGC 2 cut(s) 176, 892
AclWI GGATC 3 cut(s) 410, 693, 794
AcoI YGGCCR 1 cut(s) 54
AcsI RAATTY 1 cut(s) 166
AdeI CACNNNGTG 1 cut(s) 602
AfaI GTAC 4 cut(s) 256, 347, 771, 782
AfiI CCNNNNNNNGG 1 cut(s) 588
AgsI TTSAA 4 cut(s) 95, 122, 184, 505
AjnI CCWGG 2 cut(s) 156, 238
AjuI GAANNNNNNNTTGG 2 cut(s) 189, 221
AleI CACNNNNGTG 1 cut(s) 838
AluBI AGCT 6 cut(s) 16, 65, 74, 232, 251, 570
AluI AGCT 6 cut(s) 16, 65, 74, 232, 251, 570
Alw26I GTCTC 2 cut(s) 630, 649
AlwI GGATC 3 cut(s) 410, 693, 794
AlwNI CAGNNNCTG 1 cut(s) 573
AoxI GGCC 2 cut(s) 54, 615
ApeKI GCWGC 2 cut(s) 65, 567
ApoI RAATTY 1 cut(s) 166
Asp700I GAANNNNTTC 1 cut(s) 143
AspLEI GCGC 2 cut(s) 238, 896
AsuC2I CCSGG 1 cut(s) 924
AsuHPI GGTGA 1 cut(s) 253
AsuII TTCGAA 1 cut(s) 701
BaeGI GKGCMC 1 cut(s) 294
BanII GRGCYC 1 cut(s) 866
BbsI GAAGAC 3 cut(s) 93, 110, 668
BbvCI CCTCAGC 1 cut(s) 70
BbvI GCAGC 2 cut(s) 52, 579
BccI CCATC 6 cut(s) 46, 125, 468, 469, 500, 759
BceAI ACGGC 1 cut(s) 121
BciT130I CCWGG 2 cut(s) 158, 240
BcnI CCSGG 1 cut(s) 924
BcoDI GTCTC 2 cut(s) 630, 649
BfaI CTAG 1 cut(s) 943
BfmI CTRYAG 1 cut(s) 565
BisI GCNGC 2 cut(s) 66, 568
BlpI GCTNAGC 1 cut(s) 152
BlsI GCNGC 2 cut(s) 67, 569
Bme1390I CCNGG 3 cut(s) 158, 240, 924
BmiI GGNNCC 1 cut(s) 455
BmrFI CCNGG 3 cut(s) 158, 240, 924
BmrI ACTGGG 2 cut(s) 598, 801
BmsI GCATC 1 cut(s) 527
BmuI ACTGGG 2 cut(s) 598, 801
BoxI GACNNNNGTC 1 cut(s) 114
BpiI GAAGAC 3 cut(s) 93, 110, 668
Bpu10I CCTNAGC 1 cut(s) 70
Bpu1102I GCTNAGC 1 cut(s) 152
Bpu14I TTCGAA 1 cut(s) 701
BpuEI CTTGAG 2 cut(s) 270, 612
BpuMI CCSGG 1 cut(s) 924
BsaBI GATNNNNATC 1 cut(s) 184
BsaI GGTCTC 1 cut(s) 630
BsaJI CCNNGG 3 cut(s) 239, 680, 820
Bsc4I CCNNNNNNNGG 1 cut(s) 588
Bse118I RCCGGY 2 cut(s) 56, 710
Bse1I ACTGG 3 cut(s) 593, 626, 807
Bse8I GATNNNNATC 1 cut(s) 184
BseBI CCWGG 2 cut(s) 158, 240
BseDI CCNNGG 3 cut(s) 239, 680, 820
BseGI GGATG 1 cut(s) 480
BseJI GATNNNNATC 1 cut(s) 184
BseLI CCNNNNNNNGG 1 cut(s) 588
BseMII CTCAG 3 cut(s) 84, 166, 261
BseNI ACTGG 3 cut(s) 593, 626, 807
BseRI GAGGAG 1 cut(s) 273
BseSI GKGCMC 1 cut(s) 294
BseXI GCAGC 2 cut(s) 52, 579
Bsh1236I CGCG 4 cut(s) 164, 236, 837, 894
BshFI GGCC 2 cut(s) 56, 617
BsiSI CCGG 3 cut(s) 57, 711, 924
BslFI GGGAC 2 cut(s) 474, 812
BslI CCNNNNNNNGG 1 cut(s) 588
BsmAI GTCTC 2 cut(s) 630, 649
BsmFI GGGAC 2 cut(s) 474, 812
BsmI GAATGC 1 cut(s) 583
BsnI GGCC 2 cut(s) 56, 617
Bso31I GGTCTC 1 cut(s) 630
Bsp119I TTCGAA 1 cut(s) 701
Bsp1286I GDGCHC 2 cut(s) 294, 866
Bsp143I GATC 5 cut(s) 415, 663, 685, 733, 786
Bsp1720I GCTNAGC 1 cut(s) 152
BspACI CCGC 2 cut(s) 176, 892
BspANI GGCC 2 cut(s) 56, 617
BspCNI CTCAG 3 cut(s) 83, 165, 260
BspFNI CGCG 4 cut(s) 164, 236, 837, 894
BspHI TCATGA 2 cut(s) 48, 751
BspLI GGNNCC 1 cut(s) 455
BspMAI CTGCAG 1 cut(s) 569
BspPI GGATC 3 cut(s) 410, 693, 794
BspT104I TTCGAA 1 cut(s) 701
BspTNI GGTCTC 1 cut(s) 630
BsrFI RCCGGY 2 cut(s) 56, 710
BsrI ACTGG 3 cut(s) 593, 626, 807
BssAI RCCGGY 2 cut(s) 56, 710
BssECI CCNNGG 3 cut(s) 239, 680, 820
BssMI GATC 5 cut(s) 415, 663, 685, 733, 786
BssT1I CCWWGG 2 cut(s) 680, 820
Bst2UI CCWGG 2 cut(s) 158, 240
Bst4CI ACNGT 5 cut(s) 28, 259, 488, 643, 800
Bst6I CTCTTC 1 cut(s) 337
BstBI TTCGAA 1 cut(s) 701
BstC8I GCNNGC 3 cut(s) 58, 234, 712
BstDEI CTNAG 3 cut(s) 70, 152, 247
BstF5I GGATG 1 cut(s) 480
BstFNI CGCG 4 cut(s) 164, 236, 837, 894
BstHHI GCGC 2 cut(s) 238, 896
BstKTI GATC 5 cut(s) 418, 666, 688, 736, 789
BstMAI GTCTC 2 cut(s) 630, 649
BstMBI GATC 5 cut(s) 415, 663, 685, 733, 786
BstMWI GCNNNNNNNGC 2 cut(s) 62, 71
BstNI CCWGG 2 cut(s) 158, 240
BstPAI GACNNNNGTC 1 cut(s) 114
BstSCI CCNGG 3 cut(s) 156, 238, 922
BstSFI CTRYAG 1 cut(s) 565
BstSLI GKGCMC 1 cut(s) 294
BstUI CGCG 4 cut(s) 164, 236, 837, 894
BstV1I GCAGC 2 cut(s) 52, 579
BstV2I GAAGAC 3 cut(s) 93, 110, 668
BsuRI GGCC 2 cut(s) 56, 617
BtsCI GGATG 1 cut(s) 480
Cac8I GCNNGC 3 cut(s) 58, 234, 712
CaiI CAGNNNCTG 1 cut(s) 573
CciI TCATGA 2 cut(s) 48, 751
CfoI GCGC 2 cut(s) 238, 896
Cfr10I RCCGGY 2 cut(s) 56, 710
CseI GACGC 2 cut(s) 104, 170
Csp6I GTAC 4 cut(s) 255, 346, 770, 781
CviAII CATG 4 cut(s) 49, 220, 720, 752
CviQI GTAC 4 cut(s) 255, 346, 770, 781
DdeI CTNAG 3 cut(s) 70, 152, 247
DpnI GATC 5 cut(s) 417, 665, 687, 735, 788
DpnII GATC 5 cut(s) 415, 663, 685, 733, 786
DraIII CACNNNGTG 1 cut(s) 602
EaeI YGGCCR 1 cut(s) 54
Eam1104I CTCTTC 1 cut(s) 337
EarI CTCTTC 1 cut(s) 337
Eco130I CCWWGG 2 cut(s) 680, 820
Eco24I GRGCYC 1 cut(s) 866
Eco31I GGTCTC 1 cut(s) 630
EcoRI GAATTC 1 cut(s) 166
EcoRII CCWGG 2 cut(s) 156, 238
EcoT14I CCWWGG 2 cut(s) 680, 820
EcoT38I GRGCYC 1 cut(s) 866
ErhI CCWWGG 2 cut(s) 680, 820
FaeI CATG 4 cut(s) 52, 223, 723, 755
FaqI GGGAC 2 cut(s) 474, 812
FatI CATG 4 cut(s) 48, 219, 719, 751
FauI CCCGC 2 cut(s) 169, 899
Fnu4HI GCNGC 2 cut(s) 66, 568
FokI GGATG 1 cut(s) 487
FriOI GRGCYC 1 cut(s) 866
Fsp4HI GCNGC 2 cut(s) 66, 568
FspBI CTAG 1 cut(s) 943
GlaI GCGC 2 cut(s) 237, 895
GluI GCNGC 2 cut(s) 66, 568
HaeIII GGCC 2 cut(s) 56, 617
HapII CCGG 3 cut(s) 57, 711, 924
HgaI GACGC 2 cut(s) 104, 170
HhaI GCGC 2 cut(s) 238, 896
Hin1II CATG 4 cut(s) 52, 223, 723, 755
Hin6I GCGC 2 cut(s) 236, 894
HinP1I GCGC 2 cut(s) 236, 894
HinfI GANTC 2 cut(s) 180, 340
HpaII CCGG 3 cut(s) 57, 711, 924
HphI GGTGA 1 cut(s) 253
Hpy166II GTNNAC 1 cut(s) 348
Hpy188I TCNGA 2 cut(s) 853, 861
Hpy188III TCNNGA 6 cut(s) 49, 451, 496, 574, 752, 908
Hpy8I GTNNAC 1 cut(s) 348
Hpy99I CGWCG 1 cut(s) 515
HpyCH4III ACNGT 5 cut(s) 28, 259, 488, 643, 800
HpyCH4IV ACGT 3 cut(s) 41, 211, 510
HpyCH4V TGCA 3 cut(s) 567, 847, 884
HpyF10VI GCNNNNNNNGC 2 cut(s) 62, 71
HpyF3I CTNAG 3 cut(s) 70, 152, 247
HpySE526I ACGT 3 cut(s) 41, 211, 510
Hsp92II CATG 4 cut(s) 52, 223, 723, 755
HspAI GCGC 2 cut(s) 236, 894
KroI GCCGGC 2 cut(s) 56, 710
KroNI GCCGGC 2 cut(s) 58, 712
Kzo9I GATC 5 cut(s) 415, 663, 685, 733, 786
LmnI GCTCC 1 cut(s) 901
Lsp1109I GCAGC 2 cut(s) 52, 579
LweI GCATC 1 cut(s) 527
MaeI CTAG 1 cut(s) 943
MaeII ACGT 3 cut(s) 41, 211, 510
MaeIII GTNAC 2 cut(s) 259, 800
MalI GATC 5 cut(s) 417, 665, 687, 735, 788
MboI GATC 5 cut(s) 415, 663, 685, 733, 786
MboII GAAGA 5 cut(s) 98, 110, 270, 324, 668
MfeI CAATTG 1 cut(s) 522
MhlI GDGCHC 2 cut(s) 294, 866
MluCI AATT 7 cut(s) 76, 122, 166, 398, 444, 522, 715
MlyI GAGTC 1 cut(s) 349
MnlI CCTC 5 cut(s) 79, 128, 294, 549, 549
MroNI GCCGGC 2 cut(s) 56, 710
MroXI GAANNNNTTC 1 cut(s) 143
MseI TTAA 1 cut(s) 915
MslI CAYNNNNRTG 3 cut(s) 224, 360, 838
MspA1I CMGCKG 1 cut(s) 251
MspI CCGG 3 cut(s) 57, 711, 924
MspR9I CCNGG 3 cut(s) 158, 240, 924
MunI CAATTG 1 cut(s) 522
Mva1269I GAATGC 1 cut(s) 583
MvaI CCWGG 2 cut(s) 158, 240
MvnI CGCG 4 cut(s) 164, 236, 837, 894
MwoI GCNNNNNNNGC 2 cut(s) 62, 71
NaeI GCCGGC 2 cut(s) 58, 712
NciI CCSGG 1 cut(s) 924
NdeII GATC 5 cut(s) 415, 663, 685, 733, 786
NgoMIV GCCGGC 2 cut(s) 56, 710
NlaIII CATG 4 cut(s) 52, 223, 723, 755
NlaIV GGNNCC 1 cut(s) 455
NmuCI GTSAC 2 cut(s) 259, 800
NspV TTCGAA 1 cut(s) 701
OliI CACNNNNGTG 1 cut(s) 838
PagI TCATGA 2 cut(s) 48, 751
PctI GAATGC 1 cut(s) 583
PdiI GCCGGC 2 cut(s) 58, 712
PdmI GAANNNNTTC 1 cut(s) 143
PfeI GAWTC 1 cut(s) 180
PkrI GCNGC 2 cut(s) 67, 569
PleI GAGTC 1 cut(s) 348
PpsI GAGTC 1 cut(s) 348
PshAI GACNNNNGTC 1 cut(s) 114
Psp6I CCWGG 2 cut(s) 156, 238
PspGI CCWGG 2 cut(s) 156, 238
PspN4I GGNNCC 1 cut(s) 455
PstI CTGCAG 1 cut(s) 569
PstNI CAGNNNCTG 1 cut(s) 573
PvuII CAGCTG 1 cut(s) 251
RsaI GTAC 4 cut(s) 256, 347, 771, 782
RsaNI GTAC 4 cut(s) 255, 346, 770, 781
RseI CAYNNNNRTG 3 cut(s) 224, 360, 838
SaqAI TTAA 1 cut(s) 915
SatI GCNGC 2 cut(s) 66, 568
Sau3AI GATC 5 cut(s) 415, 663, 685, 733, 786
SchI GAGTC 1 cut(s) 349
ScrFI CCNGG 3 cut(s) 158, 240, 924
SduI GDGCHC 2 cut(s) 294, 866
SfaNI GCATC 1 cut(s) 527
SfcI CTRYAG 1 cut(s) 565
SfuI TTCGAA 1 cut(s) 701
SmiMI CAYNNNNRTG 3 cut(s) 224, 360, 838
SmlI CTYRAG 2 cut(s) 285, 627
SmoI CTYRAG 2 cut(s) 285, 627
Sse9I AATT 7 cut(s) 76, 122, 166, 398, 444, 522, 715
SsiI CCGC 2 cut(s) 176, 892
SspI AATATT 1 cut(s) 730
SspMI CTAG 1 cut(s) 943
StyD4I CCNGG 3 cut(s) 156, 238, 922
StyI CCWWGG 2 cut(s) 680, 820
TaaI ACNGT 5 cut(s) 28, 259, 488, 643, 800
TaiI ACGT 3 cut(s) 44, 214, 513
TaqI TCGA 4 cut(s) 216, 513, 701, 763
TasI AATT 7 cut(s) 76, 122, 166, 398, 444, 522, 715
TatI WGTACW 1 cut(s) 780
TfiI GAWTC 1 cut(s) 180
Tru1I TTAA 1 cut(s) 915
Tru9I TTAA 1 cut(s) 915
TseFI GTSAC 2 cut(s) 259, 800
TseI GCWGC 2 cut(s) 65, 567
Tsp45I GTSAC 2 cut(s) 259, 800
TspDTI ATGAA 4 cut(s) 37, 242, 392, 708
TspGWI ACGGA 1 cut(s) 333
XapI RAATTY 1 cut(s) 166
XmnI GAANNNNTTC 1 cut(s) 143
XspI CTAG 1 cut(s) 943
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.