RLG00000031947

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000406
Physical Location & Seq
Reverse (-)
9723856 .. 9725554
1699 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031947

Sequence Viewer

Length: 885 bp
ATGTTGAAGATCGTCTTGTTCTTTCTGTCTGTGAGTTGTTCATTCATGGCTGCTGCTTCAGCTGATAATTTCTACCGGGATTTCGACCTAACTTGGGGTGACCATCGAGCTAAGATACTCGACGGAGGAAACCTCCTCACTCTTTCTCTCGATCAAATCTCTGGCTCTGGGTTTCAGTCCAAGAGAGAATATCTTTTCGGCAGAATCGACATGCAGCTTAAGCTTGTCGCCGGCAACTCCGCCGGCACCGTCACCGCTTACTATTTGTCTTCAGAAGGGCCGAATCACGATGAGATTGATTTTGAGTTTTTGGGGAACTTGAGTGGAGATCCTTATATTGTCCATACGAACGTTTATACACAAGGGAAAGGGGGTAGAGAGCAACAATTCTACCTATGGTTCGACCCAACAAAAAACTTTCACACATACTCAATCCTTTGGAATCCCCAACGTATCATATTCTTAGTGGACAACACACCCATAAGAGTGTTCAACAACGAGGAATCAATTGGAGTCGGCTTCCCCAATAAGCAACCCATGAAGCTCTACTCGAGTCTCTGGAACGCGGACCAATGGGCAACAAAAGGCGGGCTAATCAAAACCGACTGGTCCAAGGCTCCATTCACAGCCTACTACAAAAGCTTCAATGCCAATGCCTGTGTTTGGTCTCGGGGTTCTTCATCGTGTGCCAACTCCAAAAGTACCCCAAGACCAGATGGTTTGGGCATGAACAACAAAAATGGTTGGCAGACTCAAGGGCTTAATGCACCTGGTCGAAAAAGACTGAGATGGGTGCAGAGGTACTACATGATTTATAACTATTGCACTGATTTGAAGCGATTCCCTCAGGGTCGTCCAAGGGAGTGTAGGCGTTCTAGGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_16 XET_C

Protein Analysis

295

Amino Acids

33.65

Weight (kDa)

9.41

Isoelectric Point (pI)

41.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 25 - 205 1e-66 Glycosyl hydrolases family 16
XET_C PF06955 245 - 289 6.7e-19 Xyloglucan endo-transglycosylase (XET) C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000406)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23730 AT4G14130
fragaria_vesca FvH4_3g06510 FvH4_4g12960 FvH4_6g38150 FvH4_6g38150 FvH4_6g38150 FvH4_6g38160 FvH4_6g38170 FvH4_6g38181 FvH4_6g38190
malus_domestica MD04G1020100.v1.1 MD09G1152400.v1.1 MD09G1152600.v1.1 MD09G1152700.v1.1 MD17G1139900.v1.1 MD17G1140000.v1.1
prunus_persica Prupe.1G169700_v2.0.a1 Prupe.3G171500_v2.0.a1 Prupe.3G171600_v2.0.a1 Prupe.3G171700_v2.0.a1 Prupe.3G171800_v2.0.a1 Prupe.3G172000_v2.0.a1 Prupe.3G172100_v2.0.a1 Prupe.4G072800_v2.0.a1
pyrus_communis pycom04g01620 pycom09g07160 pycom09g07220 pycom09g07230 pycom10g22480 pycom13g29190 pycom17g13320
rosa_chinensis RchiOBHm_Chr2g0151371 RchiOBHm_Chr2g0151381 RchiOBHm_Chr2g0151401 RchiOBHm_Chr2g0151421 RchiOBHm_Chr2g0151431 RchiOBHm_Chr2g0151441 RchiOBHm_Chr2g0152071 RchiOBHm_Chr4g0412651 RchiOBHm_Chr5g0012671
rosa_laevigata RLG00000008270 RLG00000020544 RLG00000020546 RLG00000020547 RLG00000020548 RLG00000020549 RLG00000020605 RLG00000031947
rosa_multiflora Rmu_co8167812.1_g000001 Rmu_co8363165.1_g000001 Rmu_co8513985.1_g000001 Rmu_sc0000940.1_g000002 Rmu_sc0000940.1_g000005 Rmu_sc0000940.1_g000006 Rmu_sc0000940.1_g000009 Rmu_sc0002516.1_g000015 Rmu_sc0002516.1_g000017 Rmu_sc0002516.1_g000018 Rmu_sc0043125.1_g000001
rosa_roxburghii Rroxscaffold_1G00063930 Rroxscaffold_2G00095990 Rroxscaffold_2G00096470 Rroxscaffold_2G00096480 Rroxscaffold_2G00096490 Rroxscaffold_2G00096500 Rroxscaffold_5G00356830
rosa_rugosa Rorug02G0425600 Rorug02G0425700 Rorug02G0425800 Rorug02G0425900 Rorug02G0430500 Rorug02G0430600 Rorug04G0115400 Rorug05G0003700
rosa_samantha Rh2BG499200 Rh2CG472700 Rh2CG472800 Rh2CG473000 Rh2CG473100 Rh2CG473300 Rh2CG477800 Rh2DG509800 Rh2DG509900 Rh2DG510100 Rh2DG510200 Rh2DG510300 Rh2DG515000 Rh4AG174700 Rh4BG174000 Rh5AG097500
rosa_wichuraiana Rw2G039850 Rw2G039860 Rw2G039870 Rw2G039880 Rw2G039890 Rw2G039900 Rw2G040400 Rw4G014650 Rw5G008540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 816
AccB1I GGYRCC 1 cut(s) 245
AccII CGCG 1 cut(s) 566
AciI CCGC 4 cut(s) 240, 255, 566, 588
AclI AACGTT 1 cut(s) 351
AclWI GGATC 1 cut(s) 323
AcuI CTGAAG 2 cut(s) 42, 255
AfaI GTAC 2 cut(s) 703, 803
AfiI CCNNNNNNNGG 2 cut(s) 94, 663
AflII CTTAAG 1 cut(s) 218
AgsI TTSAA 4 cut(s) 7, 493, 646, 835
AjnI CCWGG 1 cut(s) 769
AjuI GAANNNNNNNTTGG 2 cut(s) 605, 637
AluBI AGCT 6 cut(s) 62, 110, 217, 223, 544, 642
AluI AGCT 6 cut(s) 62, 110, 217, 223, 544, 642
Alw26I GTCTC 2 cut(s) 560, 672
AlwI GGATC 1 cut(s) 323
Ama87I CYCGRG 2 cut(s) 550, 669
AoxI GGCC 1 cut(s) 278
ApeKI GCWGC 3 cut(s) 50, 53, 214
Asp700I GAANNNNTTC 1 cut(s) 839
AspS9I GGNCC 3 cut(s) 278, 568, 609
AsuC2I CCSGG 1 cut(s) 77
AsuHPI GGTGA 2 cut(s) 110, 244
AvaI CYCGRG 2 cut(s) 550, 669
AvaII GGWCC 2 cut(s) 568, 609
AxyI CCTNAGG 1 cut(s) 846
BanI GGYRCC 1 cut(s) 245
BbsI GAAGAC 1 cut(s) 261
BbvI GCAGC 3 cut(s) 37, 40, 226
BccI CCATC 3 cut(s) 111, 710, 783
BciT130I CCWGG 1 cut(s) 771
BcnI CCSGG 1 cut(s) 77
BcoDI GTCTC 2 cut(s) 560, 672
BfaI CTAG 1 cut(s) 876
BfrI CTTAAG 1 cut(s) 218
BisI GCNGC 3 cut(s) 51, 54, 215
BlsI GCNGC 3 cut(s) 52, 55, 216
Bme1390I CCNGG 2 cut(s) 77, 771
Bme18I GGWCC 2 cut(s) 568, 609
BmeT110I CYCGRG 2 cut(s) 550, 669
BmgT120I GGNCC 3 cut(s) 278, 568, 609
BmiI GGNNCC 2 cut(s) 247, 618
BmrFI CCNGG 2 cut(s) 77, 771
BpiI GAAGAC 1 cut(s) 261
BplI GAGNNNNNCTC 2 cut(s) 117, 149
BpuEI CTTGAG 2 cut(s) 340, 738
BpuMI CCSGG 1 cut(s) 77
BsaI GGTCTC 1 cut(s) 672
BsaJI CCNNGG 2 cut(s) 612, 857
Bsc4I CCNNNNNNNGG 2 cut(s) 94, 663
Bse118I RCCGGY 2 cut(s) 230, 242
Bse1I ACTGG 1 cut(s) 611
Bse21I CCTNAGG 1 cut(s) 846
BseBI CCWGG 1 cut(s) 771
BseDI CCNNGG 2 cut(s) 612, 857
BseLI CCNNNNNNNGG 2 cut(s) 94, 663
BseMII CTCAG 2 cut(s) 776, 860
BseNI ACTGG 1 cut(s) 611
BseRI GAGGAG 1 cut(s) 125
BseXI GCAGC 3 cut(s) 37, 40, 226
BsgI GTGCAG 1 cut(s) 815
Bsh1236I CGCG 1 cut(s) 566
BshFI GGCC 1 cut(s) 280
BshNI GGYRCC 1 cut(s) 245
BsiHKCI CYCGRG 2 cut(s) 550, 669
BsiSI CCGG 3 cut(s) 76, 231, 243
BslI CCNNNNNNNGG 2 cut(s) 94, 663
BsmAI GTCTC 2 cut(s) 560, 672
BsnI GGCC 1 cut(s) 280
Bso31I GGTCTC 1 cut(s) 672
BsoBI CYCGRG 2 cut(s) 550, 669
Bsp143I GATC 3 cut(s) 9, 151, 328
BspACI CCGC 4 cut(s) 240, 255, 566, 588
BspANI GGCC 1 cut(s) 280
BspCNI CTCAG 2 cut(s) 777, 859
BspFNI CGCG 1 cut(s) 566
BspLI GGNNCC 2 cut(s) 247, 618
BspPI GGATC 1 cut(s) 323
BspT107I GGYRCC 1 cut(s) 245
BspTI CTTAAG 1 cut(s) 218
BspTNI GGTCTC 1 cut(s) 672
BsrFI RCCGGY 2 cut(s) 230, 242
BsrI ACTGG 1 cut(s) 611
BssAI RCCGGY 2 cut(s) 230, 242
BssECI CCNNGG 2 cut(s) 612, 857
BssMI GATC 3 cut(s) 9, 151, 328
BssT1I CCWWGG 2 cut(s) 612, 857
Bst2UI CCWGG 1 cut(s) 771
Bst4CI ACNGT 1 cut(s) 250
BstAFI CTTAAG 1 cut(s) 218
BstC8I GCNNGC 3 cut(s) 232, 244, 590
BstDEI CTNAG 4 cut(s) 111, 463, 785, 846
BstEII GGTNACC 1 cut(s) 98
BstFNI CGCG 1 cut(s) 566
BstKTI GATC 3 cut(s) 12, 154, 331
BstMAI GTCTC 2 cut(s) 560, 672
BstMBI GATC 3 cut(s) 9, 151, 328
BstMWI GCNNNNNNNGC 2 cut(s) 59, 220
BstNI CCWGG 1 cut(s) 771
BstNSI RCATGY 1 cut(s) 214
BstPI GGTNACC 1 cut(s) 98
BstSCI CCNGG 2 cut(s) 75, 769
BstUI CGCG 1 cut(s) 566
BstV1I GCAGC 3 cut(s) 37, 40, 226
BstV2I GAAGAC 1 cut(s) 261
BstX2I RGATCY 1 cut(s) 328
BstYI RGATCY 1 cut(s) 328
Bsu36I CCTNAGG 1 cut(s) 846
BsuRI GGCC 1 cut(s) 280
BtsIMutI CAGTG 1 cut(s) 825
Cac8I GCNNGC 3 cut(s) 232, 244, 590
Cfr10I RCCGGY 2 cut(s) 230, 242
Cfr13I GGNCC 3 cut(s) 278, 568, 609
CsiI ACCWGGT 1 cut(s) 769
Csp6I GTAC 2 cut(s) 702, 802
CviAII CATG 5 cut(s) 46, 211, 538, 727, 808
CviQI GTAC 2 cut(s) 702, 802
DdeI CTNAG 4 cut(s) 111, 463, 785, 846
DpnI GATC 3 cut(s) 11, 153, 330
DpnII GATC 3 cut(s) 9, 151, 328
EciI GGCGGA 1 cut(s) 229
Eco130I CCWWGG 2 cut(s) 612, 857
Eco31I GGTCTC 1 cut(s) 672
Eco47I GGWCC 2 cut(s) 568, 609
Eco57I CTGAAG 2 cut(s) 42, 255
Eco81I CCTNAGG 1 cut(s) 846
Eco88I CYCGRG 2 cut(s) 550, 669
Eco91I GGTNACC 1 cut(s) 98
EcoO65I GGTNACC 1 cut(s) 98
EcoRII CCWGG 1 cut(s) 769
EcoT14I CCWWGG 2 cut(s) 612, 857
ErhI CCWWGG 2 cut(s) 612, 857
FaeI CATG 5 cut(s) 49, 214, 541, 730, 811
FalI AAGNNNNNCTT 1 cut(s) 31
FatI CATG 5 cut(s) 45, 210, 537, 726, 807
FauI CCCGC 1 cut(s) 581
Fnu4HI GCNGC 3 cut(s) 51, 54, 215
Fsp4HI GCNGC 3 cut(s) 51, 54, 215
FspBI CTAG 1 cut(s) 876
GluI GCNGC 3 cut(s) 51, 54, 215
HaeIII GGCC 1 cut(s) 280
HapII CCGG 3 cut(s) 76, 231, 243
Hin1II CATG 5 cut(s) 49, 214, 541, 730, 811
HindIII AAGCTT 2 cut(s) 221, 640
HinfI GANTC 8 cut(s) 204, 283, 442, 503, 513, 553, 751, 840
HpaII CCGG 3 cut(s) 76, 231, 243
HphI GGTGA 2 cut(s) 110, 244
Hpy166II GTNNAC 1 cut(s) 469
Hpy188I TCNGA 1 cut(s) 274
Hpy188III TCNNGA 3 cut(s) 149, 287, 559
Hpy8I GTNNAC 1 cut(s) 469
Hpy99I CGWCG 1 cut(s) 125
HpyAV CCTTC 1 cut(s) 269
HpyCH4III ACNGT 1 cut(s) 250
HpyCH4IV ACGT 2 cut(s) 351, 451
HpyCH4V TGCA 4 cut(s) 214, 767, 796, 825
HpyF10VI GCNNNNNNNGC 2 cut(s) 59, 220
HpyF3I CTNAG 4 cut(s) 111, 463, 785, 846
HpySE526I ACGT 2 cut(s) 351, 451
Hsp92II CATG 5 cut(s) 49, 214, 541, 730, 811
KroI GCCGGC 2 cut(s) 230, 242
KroNI GCCGGC 2 cut(s) 232, 244
Kzo9I GATC 3 cut(s) 9, 151, 328
LmnI GCTCC 1 cut(s) 622
Lsp1109I GCAGC 3 cut(s) 37, 40, 226
MabI ACCWGGT 1 cut(s) 769
MaeI CTAG 1 cut(s) 876
MaeII ACGT 2 cut(s) 351, 451
MaeIII GTNAC 2 cut(s) 98, 250
MalI GATC 3 cut(s) 11, 153, 330
MboI GATC 3 cut(s) 9, 151, 328
MboII GAAGA 3 cut(s) 19, 261, 669
MfeI CAATTG 1 cut(s) 507
MflI RGATCY 1 cut(s) 328
MluCI AATT 3 cut(s) 67, 386, 507
MlyI GAGTC 3 cut(s) 522, 562, 745
MnlI CCTC 6 cut(s) 119, 143, 146, 493, 792, 855
MroNI GCCGGC 2 cut(s) 230, 242
MroXI GAANNNNTTC 1 cut(s) 839
MseI TTAA 2 cut(s) 219, 762
MslI CAYNNNNRTG 1 cut(s) 485
MspA1I CMGCKG 1 cut(s) 62
MspCI CTTAAG 1 cut(s) 218
MspI CCGG 3 cut(s) 76, 231, 243
MspR9I CCNGG 2 cut(s) 77, 771
MunI CAATTG 1 cut(s) 507
MvaI CCWGG 1 cut(s) 771
MvnI CGCG 1 cut(s) 566
MwoI GCNNNNNNNGC 2 cut(s) 59, 220
NaeI GCCGGC 2 cut(s) 232, 244
NciI CCSGG 1 cut(s) 77
NdeII GATC 3 cut(s) 9, 151, 328
NgoMIV GCCGGC 2 cut(s) 230, 242
NlaIII CATG 5 cut(s) 49, 214, 541, 730, 811
NlaIV GGNNCC 2 cut(s) 247, 618
NmuCI GTSAC 2 cut(s) 98, 250
NspI RCATGY 1 cut(s) 214
PaeR7I CTCGAG 1 cut(s) 550
PcsI WCGNNNNNNNCGW 1 cut(s) 204
PdiI GCCGGC 2 cut(s) 232, 244
PdmI GAANNNNTTC 1 cut(s) 839
PfeI GAWTC 5 cut(s) 204, 283, 442, 503, 840
PkrI GCNGC 3 cut(s) 52, 55, 216
PleI GAGTC 3 cut(s) 521, 561, 745
PpsI GAGTC 3 cut(s) 521, 561, 745
PsiI TTATAA 1 cut(s) 816
Psp1406I AACGTT 1 cut(s) 351
Psp6I CCWGG 1 cut(s) 769
PspEI GGTNACC 1 cut(s) 98
PspGI CCWGG 1 cut(s) 769
PspN4I GGNNCC 2 cut(s) 247, 618
PspPI GGNCC 3 cut(s) 278, 568, 609
PspXI VCTCGAGB 1 cut(s) 550
PsuI RGATCY 1 cut(s) 328
PvuII CAGCTG 1 cut(s) 62
RsaI GTAC 2 cut(s) 703, 803
RsaNI GTAC 2 cut(s) 702, 802
RseI CAYNNNNRTG 1 cut(s) 485
SaqAI TTAA 2 cut(s) 219, 762
SatI GCNGC 3 cut(s) 51, 54, 215
Sau3AI GATC 3 cut(s) 9, 151, 328
Sau96I GGNCC 3 cut(s) 278, 568, 609
SchI GAGTC 3 cut(s) 522, 562, 745
ScrFI CCNGG 2 cut(s) 77, 771
SexAI ACCWGGT 1 cut(s) 769
Sfr274I CTCGAG 1 cut(s) 550
SinI GGWCC 2 cut(s) 568, 609
SlaI CTCGAG 1 cut(s) 550
SmiMI CAYNNNNRTG 1 cut(s) 485
SmlI CTYRAG 4 cut(s) 218, 319, 550, 753
SmoI CTYRAG 4 cut(s) 218, 319, 550, 753
Sse9I AATT 3 cut(s) 67, 386, 507
SsiI CCGC 4 cut(s) 240, 255, 566, 588
SspMI CTAG 1 cut(s) 876
StyD4I CCNGG 2 cut(s) 75, 769
StyI CCWWGG 2 cut(s) 612, 857
TaaI ACNGT 1 cut(s) 250
TaiI ACGT 2 cut(s) 354, 454
TaqI TCGA 8 cut(s) 84, 106, 120, 150, 207, 402, 551, 775
TasI AATT 3 cut(s) 67, 386, 507
TfiI GAWTC 5 cut(s) 204, 283, 442, 503, 840
Tru1I TTAA 2 cut(s) 219, 762
Tru9I TTAA 2 cut(s) 219, 762
TscAI CASTG 1 cut(s) 832
TseFI GTSAC 2 cut(s) 98, 250
TseI GCWGC 3 cut(s) 50, 53, 214
Tsp45I GTSAC 2 cut(s) 98, 250
TspDTI ATGAA 5 cut(s) 30, 34, 554, 669, 743
TspGWI ACGGA 1 cut(s) 138
TspRI CASTG 1 cut(s) 832
Vha464I CTTAAG 1 cut(s) 218
VpaK11BI GGWCC 2 cut(s) 568, 609
XceI RCATGY 1 cut(s) 214
XhoI CTCGAG 1 cut(s) 550
XmnI GAANNNNTTC 1 cut(s) 839
XspI CTAG 1 cut(s) 876
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.