MD04G1020100.v1.1

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000406
Physical Location & Seq
Reverse (-)
2386634 .. 2388832
2199 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1020100.v1.1.491

Sequence Viewer

Length: 828 bp
ATGGGGATTTCTAGTTTGGTTTTGTTGGTGGGCTTGGTTTGCAGCTCTTTAATGGTAGCAACTTATGCTGGAAACTTTTACCAAGATTTTGACCTAACATGGGGTGGCAACCGCCCCAAAATATTCAAGGGAGGTCAACTTCTTTCTCTGTCTTTAGACAAAGTTTCTGGCTCTGGGTTTCAGTCCAAAAAAGAGTACCTCTTTGGGAGGATTGATATGCAGCTTAAGCTTGTTCCCGGCAACTCTGCTGGCACCGTCACCGCATACTACCTGTCCTCTCAAGGTCCTACACATGATGAAATCGACTTCGAATTCTTGGGAAATCTTAGCGGTGACCCTTACATTTTGCACACCAATATTTTCACCCAGGGCAAGGGAGATAGAGAGCAGCAGTTCTATCTCTGGTTTGACCCCTCAAAAAACTTCCACACTTATTCCGTCATCTGGAAGCCCCAACACATAATTTTCTTGGTTGACAAGACTCCCATCAGAGTATTCAAGAACGCTGAAGCAGTTGGTGTCCCATTCCCGAAGAGCCAACCCATGAGGATATACTCAAGCCTTTGGAATGCAGATGACTGGGCCACCAGAGGAGGGTTGGTGAAAACTGATTGGTCGAAGGCACCCTTCACAGCATACTACAGAAACTTCAACGTCATTGATTCCAGGTCATCCAGTTCCTTCTCCGAATGGCAGACTAACGAGCTTGATGCTCCCAGCAGAAGACGTCTGAGATGGGTGCAGAAATATTTCATGATTTACAACTATTGCACCGACTTAAAACGCTTCCCACAAGGCGCTCCACCTGAATGCAGACGATCTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_16 XET_C

Protein Analysis

276

Amino Acids

31.5

Weight (kDa)

9.2

Isoelectric Point (pI)

47.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 27 - 207 1.8e-66 Glycosyl hydrolases family 16
XET_C PF06955 229 - 271 4e-19 Xyloglucan endo-transglycosylase (XET) C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000406)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23730 AT4G14130
fragaria_vesca FvH4_3g06510 FvH4_4g12960 FvH4_6g38150 FvH4_6g38150 FvH4_6g38150 FvH4_6g38160 FvH4_6g38170 FvH4_6g38181 FvH4_6g38190
malus_domestica MD04G1020100.v1.1 MD09G1152400.v1.1 MD09G1152600.v1.1 MD09G1152700.v1.1 MD17G1139900.v1.1 MD17G1140000.v1.1
prunus_persica Prupe.1G169700_v2.0.a1 Prupe.3G171500_v2.0.a1 Prupe.3G171600_v2.0.a1 Prupe.3G171700_v2.0.a1 Prupe.3G171800_v2.0.a1 Prupe.3G172000_v2.0.a1 Prupe.3G172100_v2.0.a1 Prupe.4G072800_v2.0.a1
pyrus_communis pycom04g01620 pycom09g07160 pycom09g07220 pycom09g07230 pycom10g22480 pycom13g29190 pycom17g13320
rosa_chinensis RchiOBHm_Chr2g0151371 RchiOBHm_Chr2g0151381 RchiOBHm_Chr2g0151401 RchiOBHm_Chr2g0151421 RchiOBHm_Chr2g0151431 RchiOBHm_Chr2g0151441 RchiOBHm_Chr2g0152071 RchiOBHm_Chr4g0412651 RchiOBHm_Chr5g0012671
rosa_laevigata RLG00000008270 RLG00000020544 RLG00000020546 RLG00000020547 RLG00000020548 RLG00000020549 RLG00000020605 RLG00000031947
rosa_multiflora Rmu_co8167812.1_g000001 Rmu_co8363165.1_g000001 Rmu_co8513985.1_g000001 Rmu_sc0000940.1_g000002 Rmu_sc0000940.1_g000005 Rmu_sc0000940.1_g000006 Rmu_sc0000940.1_g000009 Rmu_sc0002516.1_g000015 Rmu_sc0002516.1_g000017 Rmu_sc0002516.1_g000018 Rmu_sc0043125.1_g000001
rosa_roxburghii Rroxscaffold_1G00063930 Rroxscaffold_2G00095990 Rroxscaffold_2G00096470 Rroxscaffold_2G00096480 Rroxscaffold_2G00096490 Rroxscaffold_2G00096500 Rroxscaffold_5G00356830
rosa_rugosa Rorug02G0425600 Rorug02G0425700 Rorug02G0425800 Rorug02G0425900 Rorug02G0430500 Rorug02G0430600 Rorug04G0115400 Rorug05G0003700
rosa_samantha Rh2BG499200 Rh2CG472700 Rh2CG472800 Rh2CG473000 Rh2CG473100 Rh2CG473300 Rh2CG477800 Rh2DG509800 Rh2DG509900 Rh2DG510100 Rh2DG510200 Rh2DG510300 Rh2DG515000 Rh4AG174700 Rh4BG174000 Rh5AG097500
rosa_wichuraiana Rw2G039850 Rw2G039860 Rw2G039870 Rw2G039880 Rw2G039890 Rw2G039900 Rw2G040400 Rw4G014650 Rw5G008540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 730
AccB1I GGYRCC 2 cut(s) 251, 622
AciI CCGC 3 cut(s) 112, 261, 330
AcsI RAATTY 1 cut(s) 311
AcuI CTGAAG 1 cut(s) 528
AcyI GRCGYC 1 cut(s) 727
AfaI GTAC 1 cut(s) 197
AfiI CCNNNNNNNGG 4 cut(s) 100, 373, 444, 594
AflII CTTAAG 1 cut(s) 224
AgsI TTSAA 3 cut(s) 127, 499, 652
AjnI CCWGG 2 cut(s) 366, 665
AluBI AGCT 4 cut(s) 45, 223, 229, 706
AluI AGCT 4 cut(s) 45, 223, 229, 706
AoxI GGCC 1 cut(s) 582
ApeKI GCWGC 3 cut(s) 42, 220, 388
ApoI RAATTY 1 cut(s) 311
Asp700I GAANNNNTTC 1 cut(s) 749
AspLEI GCGC 1 cut(s) 800
AspS9I GGNCC 2 cut(s) 284, 582
AsuC2I CCSGG 1 cut(s) 237
AsuHPI GGTGA 4 cut(s) 250, 344, 355, 613
AsuII TTCGAA 1 cut(s) 309
AvaII GGWCC 1 cut(s) 284
BanI GGYRCC 2 cut(s) 251, 622
BbsI GAAGAC 1 cut(s) 730
BbvI GCAGC 3 cut(s) 54, 232, 400
BccI CCATC 2 cut(s) 494, 729
BcgI CGANNNNNNTGC 2 cut(s) 692, 726
BciT130I CCWGG 2 cut(s) 368, 667
BcnI CCSGG 1 cut(s) 237
BfaI CTAG 2 cut(s) 12, 826
BfmI CTRYAG 1 cut(s) 640
BfoI RGCGCY 1 cut(s) 801
BfrI CTTAAG 1 cut(s) 224
BisI GCNGC 3 cut(s) 43, 221, 389
BlsI GCNGC 3 cut(s) 44, 222, 390
Bme1390I CCNGG 3 cut(s) 237, 368, 667
Bme18I GGWCC 1 cut(s) 284
BmgT120I GGNCC 2 cut(s) 284, 582
BmiI GGNNCC 2 cut(s) 253, 624
BmrFI CCNGG 3 cut(s) 237, 368, 667
BmrI ACTGGG 1 cut(s) 589
BmsI GCATC 1 cut(s) 700
BmuI ACTGGG 1 cut(s) 589
BpiI GAAGAC 1 cut(s) 730
Bpu14I TTCGAA 1 cut(s) 309
BpuEI CTTGAG 2 cut(s) 264, 541
BpuMI CCSGG 1 cut(s) 237
BsaHI GRCGYC 1 cut(s) 727
BsaJI CCNNGG 2 cut(s) 366, 367
BsaXI ACNNNNNCTCC 2 cut(s) 585, 615
Bsc4I CCNNNNNNNGG 4 cut(s) 100, 373, 444, 594
Bse1I ACTGG 2 cut(s) 584, 675
BseBI CCWGG 2 cut(s) 368, 667
BseDI CCNNGG 2 cut(s) 366, 367
BseGI GGATG 1 cut(s) 671
BseLI CCNNNNNNNGG 4 cut(s) 100, 373, 444, 594
BseMII CTCAG 1 cut(s) 722
BseNI ACTGG 2 cut(s) 584, 675
BseRI GAGGAG 1 cut(s) 606
BseXI GCAGC 3 cut(s) 54, 232, 400
BseYI CCCAGC 1 cut(s) 716
BsgI GTGCAG 1 cut(s) 761
BshFI GGCC 1 cut(s) 584
BshNI GGYRCC 2 cut(s) 251, 622
BsiSI CCGG 1 cut(s) 237
BslFI GGGAC 1 cut(s) 506
BslI CCNNNNNNNGG 4 cut(s) 100, 373, 444, 594
BsmFI GGGAC 1 cut(s) 506
BsmI GAATGC 2 cut(s) 574, 815
BsnI GGCC 1 cut(s) 584
Bsp119I TTCGAA 1 cut(s) 309
Bsp143I GATC 1 cut(s) 818
BspACI CCGC 3 cut(s) 112, 261, 330
BspANI GGCC 1 cut(s) 584
BspCNI CTCAG 1 cut(s) 723
BspHI TCATGA 1 cut(s) 753
BspLI GGNNCC 2 cut(s) 253, 624
BspQI GCTCTTC 1 cut(s) 527
BspT104I TTCGAA 1 cut(s) 309
BspT107I GGYRCC 2 cut(s) 251, 622
BspTI CTTAAG 1 cut(s) 224
BsrI ACTGG 2 cut(s) 584, 675
BssECI CCNNGG 2 cut(s) 366, 367
BssMI GATC 1 cut(s) 818
BssNI GRCGYC 1 cut(s) 727
Bst2UI CCWGG 2 cut(s) 368, 667
Bst4CI ACNGT 1 cut(s) 256
Bst6I CTCTTC 1 cut(s) 527
BstACI GRCGYC 1 cut(s) 727
BstAFI CTTAAG 1 cut(s) 224
BstAPI GCANNNNNTGC 1 cut(s) 65
BstBI TTCGAA 1 cut(s) 309
BstC8I GCNNGC 1 cut(s) 250
BstDEI CTNAG 2 cut(s) 326, 731
BstEII GGTNACC 1 cut(s) 332
BstF5I GGATG 1 cut(s) 671
BstH2I RGCGCY 1 cut(s) 801
BstHHI GCGC 1 cut(s) 800
BstKTI GATC 1 cut(s) 821
BstMBI GATC 1 cut(s) 818
BstMWI GCNNNNNNNGC 3 cut(s) 39, 65, 226
BstNI CCWGG 2 cut(s) 368, 667
BstPI GGTNACC 1 cut(s) 332
BstSCI CCNGG 3 cut(s) 235, 366, 665
BstSFI CTRYAG 1 cut(s) 640
BstV1I GCAGC 3 cut(s) 54, 232, 400
BstV2I GAAGAC 1 cut(s) 730
BsuRI GGCC 1 cut(s) 584
BtsCI GGATG 1 cut(s) 671
Cac8I GCNNGC 1 cut(s) 250
CciI TCATGA 1 cut(s) 753
CfoI GCGC 1 cut(s) 800
Cfr13I GGNCC 2 cut(s) 284, 582
Csp6I GTAC 1 cut(s) 196
CviAII CATG 4 cut(s) 99, 293, 544, 754
CviQI GTAC 1 cut(s) 196
DdeI CTNAG 2 cut(s) 326, 731
DpnI GATC 1 cut(s) 820
DpnII GATC 1 cut(s) 818
Eam1104I CTCTTC 1 cut(s) 527
EarI CTCTTC 1 cut(s) 527
Eco47I GGWCC 1 cut(s) 284
Eco57I CTGAAG 1 cut(s) 528
Eco91I GGTNACC 1 cut(s) 332
EcoO109I RGGNCCY 1 cut(s) 284
EcoO65I GGTNACC 1 cut(s) 332
EcoRI GAATTC 1 cut(s) 311
EcoRII CCWGG 2 cut(s) 366, 665
FaeI CATG 4 cut(s) 102, 296, 547, 757
FalI AAGNNNNNCTT 2 cut(s) 611, 643
FaqI GGGAC 1 cut(s) 506
FatI CATG 4 cut(s) 98, 292, 543, 753
Fnu4HI GCNGC 3 cut(s) 43, 221, 389
FokI GGATG 1 cut(s) 658
Fsp4HI GCNGC 3 cut(s) 43, 221, 389
FspBI CTAG 2 cut(s) 12, 826
GlaI GCGC 1 cut(s) 799
GluI GCNGC 3 cut(s) 43, 221, 389
GsaI CCCAGC 1 cut(s) 720
HaeII RGCGCY 1 cut(s) 801
HaeIII GGCC 1 cut(s) 584
HapII CCGG 1 cut(s) 237
HhaI GCGC 1 cut(s) 800
Hin1I GRCGYC 1 cut(s) 727
Hin1II CATG 4 cut(s) 102, 296, 547, 757
Hin6I GCGC 1 cut(s) 798
HinP1I GCGC 1 cut(s) 798
HincII GTYRAC 2 cut(s) 137, 475
HindII GTYRAC 2 cut(s) 137, 475
HindIII AAGCTT 1 cut(s) 227
HinfI GANTC 2 cut(s) 481, 662
HpaII CCGG 1 cut(s) 237
HphI GGTGA 4 cut(s) 250, 344, 355, 613
Hpy166II GTNNAC 2 cut(s) 137, 475
Hpy188I TCNGA 3 cut(s) 491, 688, 732
Hpy188III TCNNGA 4 cut(s) 445, 499, 529, 754
Hpy8I GTNNAC 2 cut(s) 137, 475
HpyAV CCTTC 3 cut(s) 613, 637, 691
HpyCH4III ACNGT 1 cut(s) 256
HpyCH4IV ACGT 2 cut(s) 654, 727
HpyCH4V TGCA 7 cut(s) 42, 220, 349, 572, 742, 771, 813
HpyF10VI GCNNNNNNNGC 3 cut(s) 39, 65, 226
HpyF3I CTNAG 2 cut(s) 326, 731
HpySE526I ACGT 2 cut(s) 654, 727
Hsp92I GRCGYC 1 cut(s) 727
Hsp92II CATG 4 cut(s) 102, 296, 547, 757
HspAI GCGC 1 cut(s) 798
Kzo9I GATC 1 cut(s) 818
LguI GCTCTTC 1 cut(s) 527
LmnI GCTCC 2 cut(s) 718, 805
Lsp1109I GCAGC 3 cut(s) 54, 232, 400
LweI GCATC 1 cut(s) 700
MaeI CTAG 2 cut(s) 12, 826
MaeII ACGT 2 cut(s) 654, 727
MaeIII GTNAC 2 cut(s) 256, 332
MalI GATC 1 cut(s) 820
MboI GATC 1 cut(s) 818
MboII GAAGA 2 cut(s) 544, 735
MluCI AATT 2 cut(s) 311, 462
MlyI GAGTC 1 cut(s) 475
MnlI CCTC 8 cut(s) 125, 201, 209, 286, 424, 540, 584, 587
MroXI GAANNNNTTC 1 cut(s) 749
MseI TTAA 3 cut(s) 50, 225, 779
MslI CAYNNNNRTG 1 cut(s) 808
MspCI CTTAAG 1 cut(s) 224
MspI CCGG 1 cut(s) 237
MspR9I CCNGG 3 cut(s) 237, 368, 667
Mva1269I GAATGC 2 cut(s) 574, 815
MvaI CCWGG 2 cut(s) 368, 667
MwoI GCNNNNNNNGC 3 cut(s) 39, 65, 226
NciI CCSGG 1 cut(s) 237
NdeII GATC 1 cut(s) 818
NlaIII CATG 4 cut(s) 102, 296, 547, 757
NlaIV GGNNCC 2 cut(s) 253, 624
NmuCI GTSAC 2 cut(s) 256, 332
NspV TTCGAA 1 cut(s) 309
PagI TCATGA 1 cut(s) 753
PasI CCCWGGG 1 cut(s) 367
PciSI GCTCTTC 1 cut(s) 527
PctI GAATGC 2 cut(s) 574, 815
PdmI GAANNNNTTC 1 cut(s) 749
PfeI GAWTC 1 cut(s) 662
PkrI GCNGC 3 cut(s) 44, 222, 390
PleI GAGTC 1 cut(s) 475
PpsI GAGTC 1 cut(s) 475
PpuMI RGGWCCY 1 cut(s) 284
Psp5II RGGWCCY 1 cut(s) 284
Psp6I CCWGG 2 cut(s) 366, 665
PspEI GGTNACC 1 cut(s) 332
PspFI CCCAGC 1 cut(s) 716
PspGI CCWGG 2 cut(s) 366, 665
PspN4I GGNNCC 2 cut(s) 253, 624
PspPI GGNCC 2 cut(s) 284, 582
PspPPI RGGWCCY 1 cut(s) 284
RsaI GTAC 1 cut(s) 197
RsaNI GTAC 1 cut(s) 196
RseI CAYNNNNRTG 1 cut(s) 808
SapI GCTCTTC 1 cut(s) 527
SaqAI TTAA 3 cut(s) 50, 225, 779
SatI GCNGC 3 cut(s) 43, 221, 389
Sau3AI GATC 1 cut(s) 818
Sau96I GGNCC 2 cut(s) 284, 582
SchI GAGTC 1 cut(s) 475
ScrFI CCNGG 3 cut(s) 237, 368, 667
SfaNI GCATC 1 cut(s) 700
SfcI CTRYAG 1 cut(s) 640
SfuI TTCGAA 1 cut(s) 309
SinI GGWCC 1 cut(s) 284
SmiMI CAYNNNNRTG 1 cut(s) 808
SmlI CTYRAG 3 cut(s) 224, 279, 556
SmoI CTYRAG 3 cut(s) 224, 279, 556
Sse9I AATT 2 cut(s) 311, 462
SsiI CCGC 3 cut(s) 112, 261, 330
SspI AATATT 3 cut(s) 123, 358, 749
SspMI CTAG 2 cut(s) 12, 826
StyD4I CCNGG 3 cut(s) 235, 366, 665
TaaI ACNGT 1 cut(s) 256
TaiI ACGT 2 cut(s) 657, 730
TaqI TCGA 3 cut(s) 303, 309, 617
TasI AATT 2 cut(s) 311, 462
TfiI GAWTC 1 cut(s) 662
Tru1I TTAA 3 cut(s) 50, 225, 779
Tru9I TTAA 3 cut(s) 50, 225, 779
TseFI GTSAC 2 cut(s) 256, 332
TseI GCWGC 3 cut(s) 42, 220, 388
Tsp45I GTSAC 2 cut(s) 256, 332
TspDTI ATGAA 2 cut(s) 312, 742
TspGWI ACGGA 1 cut(s) 427
Vha464I CTTAAG 1 cut(s) 224
VpaK11BI GGWCC 1 cut(s) 284
XapI RAATTY 1 cut(s) 311
XcmI CCANNNNNNNNNTGG 1 cut(s) 595
XmnI GAANNNNTTC 1 cut(s) 749
XspI CTAG 2 cut(s) 12, 826
ZraI GACGTC 1 cut(s) 728
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.