Rroxscaffold_1G00063930

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000406
Physical Location & Seq
Forward (+)
85799722 .. 85801376
1655 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00063930.1

Sequence Viewer

Length: 756 bp
ATGTTGAAGATCGTCTTGTTCTTTCTGTCTGTGAGTTGTTCATTCATGGCTGCTGCTTCAGCTGGTAATTTCTACCGGGATTTCGACCTAACTTGGGGTGACCATCGAGCTAAGATACTCGACGGAGGAAACCTCCTCACTCTTTCTCTCGACCAAATCTCTGGCTCTGGGTTTCAGTCCAAGAGAGAATATCTTTTCGGCAGGATCGACATGCAGCTTAAGCTTGTCGCCGGCAACTCCGCCGGCACCGTCACCGCCTACTATGACAAAAATGATTATTTTACAAATACTTATTTTGTTACTCATACTGGTGCTTACAGAGTTGGAAGATTCTTAGTGGATAACACACCCATAAGAGTGTTCAACAACGAGGAATCAATTGGAGTTGGCTTCCCCAAGAAGCAACCCATGAAGCTCTACTCGAGTCTCTGGAACGCTGACCAATGGGCAACAAAAGGCGGGCTAATCAAAACCGACTGGTCCAAGGCTCCATTCACAGCCTACTACAAAAGCTTCAACGCCAATGCCTGTGTTTGGTCTCGGGGTTCTTCATCGTGTGCCAACTCCAAAAGTACCCCAAGACCATCTGGCTTCGGCATCAACAACAACAATGGTTGGCAGACTCAAGGGCTTAATGCACCTGGTCGAAGAAGACTGAGATGGGTGCAGAGGTACTACATGATTTATAACTATTGCACTGATTTGAAGCGATTCCCTCAGGGTCGTCCAAGGGAGTGTAGGCGTTCTAGGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_16 XET_C

Protein Analysis

251

Amino Acids

28.61

Weight (kDa)

9.9

Isoelectric Point (pI)

40.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 25 - 90 1.4e-12 Glycosyl hydrolases family 16
Glyco_hydro_16 PF00722 110 - 162 8.7e-14 Glycosyl hydrolases family 16
XET_C PF06955 202 - 246 3.8e-19 Xyloglucan endo-transglycosylase (XET) C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000406)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23730 AT4G14130
fragaria_vesca FvH4_3g06510 FvH4_4g12960 FvH4_6g38150 FvH4_6g38150 FvH4_6g38150 FvH4_6g38160 FvH4_6g38170 FvH4_6g38181 FvH4_6g38190
malus_domestica MD04G1020100.v1.1 MD09G1152400.v1.1 MD09G1152600.v1.1 MD09G1152700.v1.1 MD17G1139900.v1.1 MD17G1140000.v1.1
prunus_persica Prupe.1G169700_v2.0.a1 Prupe.3G171500_v2.0.a1 Prupe.3G171600_v2.0.a1 Prupe.3G171700_v2.0.a1 Prupe.3G171800_v2.0.a1 Prupe.3G172000_v2.0.a1 Prupe.3G172100_v2.0.a1 Prupe.4G072800_v2.0.a1
pyrus_communis pycom04g01620 pycom09g07160 pycom09g07220 pycom09g07230 pycom10g22480 pycom13g29190 pycom17g13320
rosa_chinensis RchiOBHm_Chr2g0151371 RchiOBHm_Chr2g0151381 RchiOBHm_Chr2g0151401 RchiOBHm_Chr2g0151421 RchiOBHm_Chr2g0151431 RchiOBHm_Chr2g0151441 RchiOBHm_Chr2g0152071 RchiOBHm_Chr4g0412651 RchiOBHm_Chr5g0012671
rosa_laevigata RLG00000008270 RLG00000020544 RLG00000020546 RLG00000020547 RLG00000020548 RLG00000020549 RLG00000020605 RLG00000031947
rosa_multiflora Rmu_co8167812.1_g000001 Rmu_co8363165.1_g000001 Rmu_co8513985.1_g000001 Rmu_sc0000940.1_g000002 Rmu_sc0000940.1_g000005 Rmu_sc0000940.1_g000006 Rmu_sc0000940.1_g000009 Rmu_sc0002516.1_g000015 Rmu_sc0002516.1_g000017 Rmu_sc0002516.1_g000018 Rmu_sc0043125.1_g000001
rosa_roxburghii Rroxscaffold_1G00063930 Rroxscaffold_2G00095990 Rroxscaffold_2G00096470 Rroxscaffold_2G00096480 Rroxscaffold_2G00096490 Rroxscaffold_2G00096500 Rroxscaffold_5G00356830
rosa_rugosa Rorug02G0425600 Rorug02G0425700 Rorug02G0425800 Rorug02G0425900 Rorug02G0430500 Rorug02G0430600 Rorug04G0115400 Rorug05G0003700
rosa_samantha Rh2BG499200 Rh2CG472700 Rh2CG472800 Rh2CG473000 Rh2CG473100 Rh2CG473300 Rh2CG477800 Rh2DG509800 Rh2DG509900 Rh2DG510100 Rh2DG510200 Rh2DG510300 Rh2DG515000 Rh4AG174700 Rh4BG174000 Rh5AG097500
rosa_wichuraiana Rw2G039850 Rw2G039860 Rw2G039870 Rw2G039880 Rw2G039890 Rw2G039900 Rw2G040400 Rw4G014650 Rw5G008540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 687
AccB1I GGYRCC 1 cut(s) 245
AciI CCGC 3 cut(s) 240, 255, 459
AclWI GGATC 1 cut(s) 212
AcuI CTGAAG 1 cut(s) 42
AfaI GTAC 2 cut(s) 574, 674
AfiI CCNNNNNNNGG 2 cut(s) 94, 534
AflII CTTAAG 1 cut(s) 218
AgsI TTSAA 4 cut(s) 7, 364, 517, 706
AjnI CCWGG 1 cut(s) 640
AjuI GAANNNNNNNTTGG 2 cut(s) 476, 508
AluBI AGCT 6 cut(s) 62, 110, 217, 223, 415, 513
AluI AGCT 6 cut(s) 62, 110, 217, 223, 415, 513
Alw26I GTCTC 2 cut(s) 431, 543
AlwI GGATC 1 cut(s) 212
Ama87I CYCGRG 2 cut(s) 421, 540
ApeKI GCWGC 3 cut(s) 50, 53, 214
Asp700I GAANNNNTTC 1 cut(s) 710
AspS9I GGNCC 1 cut(s) 480
AsuC2I CCSGG 1 cut(s) 77
AsuHPI GGTGA 2 cut(s) 110, 244
AvaI CYCGRG 2 cut(s) 421, 540
AvaII GGWCC 1 cut(s) 480
AxyI CCTNAGG 1 cut(s) 717
BanI GGYRCC 1 cut(s) 245
BbsI GAAGAC 1 cut(s) 658
BbvI GCAGC 3 cut(s) 37, 40, 226
BccI CCATC 3 cut(s) 111, 592, 654
BciT130I CCWGG 1 cut(s) 642
BcnI CCSGG 1 cut(s) 77
BcoDI GTCTC 2 cut(s) 431, 543
BfaI CTAG 1 cut(s) 747
BfrI CTTAAG 1 cut(s) 218
BisI GCNGC 3 cut(s) 51, 54, 215
BlsI GCNGC 3 cut(s) 52, 55, 216
Bme1390I CCNGG 2 cut(s) 77, 642
Bme18I GGWCC 1 cut(s) 480
BmeT110I CYCGRG 2 cut(s) 421, 540
BmgT120I GGNCC 1 cut(s) 480
BmiI GGNNCC 2 cut(s) 247, 489
BmrFI CCNGG 2 cut(s) 77, 642
BmsI GCATC 1 cut(s) 606
BpiI GAAGAC 1 cut(s) 658
BplI GAGNNNNNCTC 2 cut(s) 117, 149
BpuEI CTTGAG 1 cut(s) 609
BpuMI CCSGG 1 cut(s) 77
BsaI GGTCTC 1 cut(s) 543
BsaJI CCNNGG 2 cut(s) 483, 728
Bsc4I CCNNNNNNNGG 2 cut(s) 94, 534
Bse118I RCCGGY 2 cut(s) 230, 242
Bse1I ACTGG 2 cut(s) 313, 482
Bse21I CCTNAGG 1 cut(s) 717
BseBI CCWGG 1 cut(s) 642
BseDI CCNNGG 2 cut(s) 483, 728
BseLI CCNNNNNNNGG 2 cut(s) 94, 534
BseMII CTCAG 2 cut(s) 647, 731
BseNI ACTGG 2 cut(s) 313, 482
BseRI GAGGAG 1 cut(s) 125
BseXI GCAGC 3 cut(s) 37, 40, 226
BsgI GTGCAG 1 cut(s) 686
BshNI GGYRCC 1 cut(s) 245
BsiHKCI CYCGRG 2 cut(s) 421, 540
BsiSI CCGG 3 cut(s) 76, 231, 243
BslI CCNNNNNNNGG 2 cut(s) 94, 534
BsmAI GTCTC 2 cut(s) 431, 543
Bso31I GGTCTC 1 cut(s) 543
BsoBI CYCGRG 2 cut(s) 421, 540
Bsp143I GATC 2 cut(s) 9, 204
BspACI CCGC 3 cut(s) 240, 255, 459
BspCNI CTCAG 2 cut(s) 648, 730
BspLI GGNNCC 2 cut(s) 247, 489
BspPI GGATC 1 cut(s) 212
BspT107I GGYRCC 1 cut(s) 245
BspTI CTTAAG 1 cut(s) 218
BspTNI GGTCTC 1 cut(s) 543
BsrFI RCCGGY 2 cut(s) 230, 242
BsrI ACTGG 2 cut(s) 313, 482
BssAI RCCGGY 2 cut(s) 230, 242
BssECI CCNNGG 2 cut(s) 483, 728
BssMI GATC 2 cut(s) 9, 204
BssT1I CCWWGG 2 cut(s) 483, 728
Bst2UI CCWGG 1 cut(s) 642
Bst4CI ACNGT 1 cut(s) 250
BstAFI CTTAAG 1 cut(s) 218
BstC8I GCNNGC 3 cut(s) 232, 244, 461
BstDEI CTNAG 4 cut(s) 111, 334, 656, 717
BstEII GGTNACC 1 cut(s) 98
BstKTI GATC 2 cut(s) 12, 207
BstMAI GTCTC 2 cut(s) 431, 543
BstMBI GATC 2 cut(s) 9, 204
BstMWI GCNNNNNNNGC 2 cut(s) 59, 220
BstNI CCWGG 1 cut(s) 642
BstNSI RCATGY 1 cut(s) 214
BstPI GGTNACC 1 cut(s) 98
BstSCI CCNGG 2 cut(s) 75, 640
BstV1I GCAGC 3 cut(s) 37, 40, 226
BstV2I GAAGAC 1 cut(s) 658
BstXI CCANNNNNNTGG 1 cut(s) 161
Bsu36I CCTNAGG 1 cut(s) 717
BtsIMutI CAGTG 1 cut(s) 696
Cac8I GCNNGC 3 cut(s) 232, 244, 461
Cfr10I RCCGGY 2 cut(s) 230, 242
Cfr13I GGNCC 1 cut(s) 480
CsiI ACCWGGT 1 cut(s) 640
Csp6I GTAC 2 cut(s) 573, 673
CviAII CATG 4 cut(s) 46, 211, 409, 679
CviQI GTAC 2 cut(s) 573, 673
DdeI CTNAG 4 cut(s) 111, 334, 656, 717
DpnI GATC 2 cut(s) 11, 206
DpnII GATC 2 cut(s) 9, 204
EciI GGCGGA 1 cut(s) 229
Eco130I CCWWGG 2 cut(s) 483, 728
Eco31I GGTCTC 1 cut(s) 543
Eco47I GGWCC 1 cut(s) 480
Eco57I CTGAAG 1 cut(s) 42
Eco81I CCTNAGG 1 cut(s) 717
Eco88I CYCGRG 2 cut(s) 421, 540
Eco91I GGTNACC 1 cut(s) 98
EcoO65I GGTNACC 1 cut(s) 98
EcoRII CCWGG 1 cut(s) 640
EcoT14I CCWWGG 2 cut(s) 483, 728
ErhI CCWWGG 2 cut(s) 483, 728
FaeI CATG 4 cut(s) 49, 214, 412, 682
FaiI YATR 8 cut(s) 47, 212, 264, 306, 353, 410, 680, 687
FalI AAGNNNNNCTT 1 cut(s) 31
FatI CATG 4 cut(s) 45, 210, 408, 678
FauI CCCGC 1 cut(s) 452
Fnu4HI GCNGC 3 cut(s) 51, 54, 215
Fsp4HI GCNGC 3 cut(s) 51, 54, 215
FspBI CTAG 1 cut(s) 747
GluI GCNGC 3 cut(s) 51, 54, 215
HapII CCGG 3 cut(s) 76, 231, 243
Hin1II CATG 4 cut(s) 49, 214, 412, 682
HindIII AAGCTT 2 cut(s) 221, 511
HinfI GANTC 5 cut(s) 330, 374, 424, 622, 711
HpaII CCGG 3 cut(s) 76, 231, 243
HphI GGTGA 2 cut(s) 110, 244
Hpy188III TCNNGA 2 cut(s) 149, 430
Hpy99I CGWCG 1 cut(s) 125
HpyCH4III ACNGT 1 cut(s) 250
HpyCH4V TGCA 4 cut(s) 214, 638, 667, 696
HpyF10VI GCNNNNNNNGC 2 cut(s) 59, 220
HpyF3I CTNAG 4 cut(s) 111, 334, 656, 717
Hsp92II CATG 4 cut(s) 49, 214, 412, 682
KroI GCCGGC 2 cut(s) 230, 242
KroNI GCCGGC 2 cut(s) 232, 244
Kzo9I GATC 2 cut(s) 9, 204
LmnI GCTCC 1 cut(s) 493
Lsp1109I GCAGC 3 cut(s) 37, 40, 226
LweI GCATC 1 cut(s) 606
MabI ACCWGGT 1 cut(s) 640
MaeI CTAG 1 cut(s) 747
MaeIII GTNAC 3 cut(s) 98, 250, 298
MalI GATC 2 cut(s) 11, 206
MboI GATC 2 cut(s) 9, 204
MboII GAAGA 5 cut(s) 19, 339, 540, 660, 663
MfeI CAATTG 1 cut(s) 378
MluCI AATT 2 cut(s) 67, 378
MlyI GAGTC 2 cut(s) 433, 616
MmeI TCCRAC 1 cut(s) 304
MnlI CCTC 6 cut(s) 119, 143, 146, 364, 663, 726
MroNI GCCGGC 2 cut(s) 230, 242
MroXI GAANNNNTTC 1 cut(s) 710
MseI TTAA 2 cut(s) 219, 633
MslI CAYNNNNRTG 2 cut(s) 309, 356
MspA1I CMGCKG 1 cut(s) 62
MspCI CTTAAG 1 cut(s) 218
MspI CCGG 3 cut(s) 76, 231, 243
MspR9I CCNGG 2 cut(s) 77, 642
MunI CAATTG 1 cut(s) 378
MvaI CCWGG 1 cut(s) 642
MwoI GCNNNNNNNGC 2 cut(s) 59, 220
NaeI GCCGGC 2 cut(s) 232, 244
NciI CCSGG 1 cut(s) 77
NdeII GATC 2 cut(s) 9, 204
NgoMIV GCCGGC 2 cut(s) 230, 242
NlaIII CATG 4 cut(s) 49, 214, 412, 682
NlaIV GGNNCC 2 cut(s) 247, 489
NmuCI GTSAC 2 cut(s) 98, 250
NspI RCATGY 1 cut(s) 214
PaeR7I CTCGAG 1 cut(s) 421
PcsI WCGNNNNNNNCGW 1 cut(s) 204
PdiI GCCGGC 2 cut(s) 232, 244
PdmI GAANNNNTTC 1 cut(s) 710
PfeI GAWTC 3 cut(s) 330, 374, 711
PkrI GCNGC 3 cut(s) 52, 55, 216
PleI GAGTC 2 cut(s) 432, 616
PpsI GAGTC 2 cut(s) 432, 616
PsiI TTATAA 1 cut(s) 687
Psp6I CCWGG 1 cut(s) 640
PspEI GGTNACC 1 cut(s) 98
PspGI CCWGG 1 cut(s) 640
PspN4I GGNNCC 2 cut(s) 247, 489
PspPI GGNCC 1 cut(s) 480
PspXI VCTCGAGB 1 cut(s) 421
PvuII CAGCTG 1 cut(s) 62
RsaI GTAC 2 cut(s) 574, 674
RsaNI GTAC 2 cut(s) 573, 673
RseI CAYNNNNRTG 2 cut(s) 309, 356
SaqAI TTAA 2 cut(s) 219, 633
SatI GCNGC 3 cut(s) 51, 54, 215
Sau3AI GATC 2 cut(s) 9, 204
Sau96I GGNCC 1 cut(s) 480
SchI GAGTC 2 cut(s) 433, 616
ScrFI CCNGG 2 cut(s) 77, 642
SexAI ACCWGGT 1 cut(s) 640
SfaNI GCATC 1 cut(s) 606
Sfr274I CTCGAG 1 cut(s) 421
SinI GGWCC 1 cut(s) 480
SlaI CTCGAG 1 cut(s) 421
SmiMI CAYNNNNRTG 2 cut(s) 309, 356
SmlI CTYRAG 3 cut(s) 218, 421, 624
SmoI CTYRAG 3 cut(s) 218, 421, 624
Sse9I AATT 2 cut(s) 67, 378
SsiI CCGC 3 cut(s) 240, 255, 459
SspMI CTAG 1 cut(s) 747
StyD4I CCNGG 2 cut(s) 75, 640
StyI CCWWGG 2 cut(s) 483, 728
TaaI ACNGT 1 cut(s) 250
TaqI TCGA 7 cut(s) 84, 106, 120, 150, 207, 422, 646
TasI AATT 2 cut(s) 67, 378
TfiI GAWTC 3 cut(s) 330, 374, 711
Tru1I TTAA 2 cut(s) 219, 633
Tru9I TTAA 2 cut(s) 219, 633
TscAI CASTG 1 cut(s) 703
TseFI GTSAC 2 cut(s) 98, 250
TseI GCWGC 3 cut(s) 50, 53, 214
Tsp45I GTSAC 2 cut(s) 98, 250
TspDTI ATGAA 4 cut(s) 30, 34, 425, 540
TspGWI ACGGA 1 cut(s) 138
TspRI CASTG 1 cut(s) 703
Vha464I CTTAAG 1 cut(s) 218
VpaK11BI GGWCC 1 cut(s) 480
XceI RCATGY 1 cut(s) 214
XhoI CTCGAG 1 cut(s) 421
XmnI GAANNNNTTC 1 cut(s) 710
XspI CTAG 1 cut(s) 747
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.