AT5G05320

Zeaxanthin epoxidase, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0000348
Physical Location & Seq
Reverse (-)
1574862 .. 1576731
1870 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G05320.1

Sequence Viewer

Length: 1221 bp
ATGGAAGCTGAAAGTACCCAAGATATCATCATCGTCGGAGCTGGAATCTCCGGCCTTGCCACTGCACTTGGGCTCCACAGGCTTGGGATCAGAAGCATAGTGCTGGAATCTTCTGAGCAGCTGAGAGCAACAGGATTTGCACTTTCATTATATTTTAATGCTTGGAAGGCCATGGAAGCTCTCGGTATTTCTCAGCATATTCGCAGTCTCGGTGATCGCTTTCAAGGATGGGTGGTCAGACCCATTTCTGCAGGAGATCCTCCTAAAGAAATGTTATTTCCAGAATCTGAAGAATATGAGGTTCGATGCGTACAGAGGAAGCTCTTGTTAGACGCTCTAGCGGGCGAATTGCCTCAAGGGACCATACGGTTCTCATCTAAGCTTGTTCACATCGAATTGTCCGGACACTACAAGATGGTTCATCTCTCCGACGGGACTATACTTAAAACCAAGGTTTTGGTAGGGTGTGATGGAGTGAAGTCAGTGGTTGGTAAGTGGCTAGGCTTCAAGAATCCGGTTAAAACTTCCCGTGTAGCAATCCGCGGGATCGCTCATTTCCAGACAGGCCACGAATTAGGGAGAAGGTTCTTTCAGTTTTATGGCAACGGTGTTCGTTCCGGTTTCATCTCATGTGACCAAAACACTGTCTACTGGTTCCTAACCCACACCTCTACTGATTTAGATAAGAAAAATCATCAGAAGATCAAACAGTTTGTGCTGACCAAGATCAAAGACTTGCCTGACAACATCAAGAGTATCCTGGAGACCACTGATCTTGATAGTTTGGTGATGAATCCACTCATGTATCGACCTCCCTGGGAACTTCTTTGGGCAAACATTGCAAAAGACAACGTATGTGTTGCAGGGGATGCACTTCACCCAATGACTCCTGATATTGGACAAGGTGGTTGCTCGGCGATGGAGGACGGAGTTATCCTCGCTCGTTGTCTCGGTGAAGCAATGAAAGCTAAGAATATGAAAGGTGAAACAGAAGATGAGAACGAGAGTTATAGGCGGATTGAAGATGGTTTGAAGAAGTATGCAGGATCGAGGAAATGGAGAAGCATTGATCTTATAACTACATCATATACAGTAGGTTTCATACAGCAGAGCAGAGGCAAGTGGATGACCCTGTTTAGAGACAAGTTCATGTCCTCTTTCCTTTCTTGGTTGCGTGTGAAAAAGTCTCATTTCAACTGTGGAAGACTTAGCCATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002239 GO:0003674 GO:0003824 GO:0004497 GO:0008150 GO:0008152 GO:0009058 GO:0009605 GO:0009607 GO:0016491 GO:0019748 GO:0043207 GO:0044550 GO:0050896 GO:0051704 GO:0051707 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAD_binding_3

Protein Analysis

406

Amino Acids

45.61

Weight (kDa)

9.09

Isoelectric Point (pI)

38.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_3 PF01494 8 - 322 4.9e-21 FAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000348)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G38540 AT5G05320
fragaria_vesca FvH4_1g12561 FvH4_1g12581 FvH4_2g15511 FvH4_2g15511 FvH4_2g15511 FvH4_2g15511 FvH4_2g15511 FvH4_2g15511 FvH4_2g15511 FvH4_2g15511 FvH4_2g15511 FvH4_3g33121 FvH4_3g33122 FvH4_4g08974 FvH4_4g09610 FvH4_7g17841
malus_domestica MD05G1123600.v1.1 MD05G1123700.v1.1
prunus_persica Prupe.8G167400_v2.0.a1 Prupe.8G167500_v2.0.a1 Prupe.8G167700_v2.0.a1 Prupe.8G167700_v2.0.a1
pyrus_communis pycom05g11780 pycom05g11790
rosa_chinensis RchiOBHm_Chr6g0278521 RchiOBHm_Chr6g0278531 RchiOBHm_Chr6g0278551 RchiOBHm_Chr6g0278591 RchiOBHm_Chr6g0278611 RchiOBHm_Chr6g0278671 RchiOBHm_Chr6g0278691 RchiOBHm_Chr6g0278701
rosa_laevigata RLG00000013200 RLG00000013201 RLG00000013203 RLG00000013205 RLG00000013206 RLG00000013210 RLG00000013211 RLG00000013213 RLG00000013216 RLG00000013217
rosa_multiflora Rmu_sc0000258.1_g000072 Rmu_sc0000258.1_g000073 Rmu_sc0000258.1_g000082 Rmu_sc0000258.1_g000083 Rmu_sc0000258.1_g000090 Rmu_sc0000258.1_g000094 Rmu_sc0000258.1_g000096 Rmu_sc0002777.1_g000006 Rmu_sc0002777.1_g000011 Rmu_sc0002777.1_g000019 Rmu_sc0006193.1_g000001 Rmu_sc0034228.1_g000001 Rmu_ssc0000042.1_g000052 Rmu_ssc0000144.1_g000014
rosa_roxburghii Rroxscaffold_7G00189700 Rroxscaffold_7G00189710 Rroxscaffold_7G00189720 Rroxscaffold_7G00189730 Rroxscaffold_7G00189770 Rroxscaffold_7G00189800 Rroxscaffold_7G00189820 Rroxscaffold_7G00189960 Rroxscaffold_7G00190070 Rroxscaffold_7G00190090 Rroxscaffold_7G00190140 Rroxscaffold_7G00190160 Rroxscaffold_7G00190170 Rroxscaffold_7G00190270 Rroxscaffold_7G00190280 Rroxscaffold_7G00190300 Rroxscaffold_7G00190310
rosa_rugosa Rorug02G0222700 Rorug06G0117300 Rorug06G0117400 Rorug06G0117700 Rorug06G0117900 Rorug06G0118000 Rorug06G0118100 Rorug06G0118200
rosa_samantha Rh1BG066900 Rh2BG292300 Rh6BG231700 Rh6BG231800 Rh6BG232000 Rh6BG232300 Rh6BG232500 Rh6CG234100 Rh6CG234200 Rh6CG234300 Rh6CG234700 Rh6DG225400 Rh6DG225500 Rh6DG225800 Rh6DG226000 Rh6DG226300
rosa_wichuraiana Rw0G003090 Rw0G003690 Rw0G021770 Rw4G037090 Rw6G019870 Rw6G019890 Rw6G019900 Rw6G019910 Rw6G019920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1076
AccI GTMKAC 1 cut(s) 648
AccII CGCG 1 cut(s) 543
AccIII TCCGGA 1 cut(s) 401
AciI CCGC 4 cut(s) 341, 541, 543, 1015
AclWI GGATC 4 cut(s) 95, 251, 554, 1054
AcuI CTGAAG 1 cut(s) 309
AfaI GTAC 2 cut(s) 16, 312
AfiI CCNNNNNNNGG 1 cut(s) 896
AgsI TTSAA 5 cut(s) 224, 508, 1022, 1033, 1195
AjnI CCWGG 2 cut(s) 759, 815
AluBI AGCT 7 cut(s) 8, 41, 121, 179, 322, 382, 968
AluI AGCT 7 cut(s) 8, 41, 121, 179, 322, 382, 968
Alw26I GTCTC 5 cut(s) 212, 758, 953, 1134, 1191
AlwI GGATC 4 cut(s) 95, 251, 554, 1054
AlwNI CAGNNNCTG 1 cut(s) 287
Aor13HI TCCGGA 1 cut(s) 401
AoxI GGCC 3 cut(s) 52, 168, 565
ApeKI GCWGC 1 cut(s) 118
AspS9I GGNCC 1 cut(s) 360
AsuHPI GGTGA 5 cut(s) 224, 799, 869, 965, 995
AvaII GGWCC 1 cut(s) 360
BaeI ACNNNNGTAYC 2 cut(s) 788, 821
BanII GRGCYC 1 cut(s) 75
BbsI GAAGAC 1 cut(s) 1210
BbvI GCAGC 1 cut(s) 130
BccI CCATC 5 cut(s) 222, 409, 464, 913, 1019
BciT130I CCWGG 2 cut(s) 761, 817
BciVI GTATCC 1 cut(s) 767
BcoDI GTCTC 5 cut(s) 212, 758, 953, 1134, 1191
BfaI CTAG 2 cut(s) 338, 500
BfmI CTRYAG 1 cut(s) 249
BfuI GTATCC 1 cut(s) 767
BisI GCNGC 1 cut(s) 119
BlsI GCNGC 1 cut(s) 120
Bme1390I CCNGG 2 cut(s) 761, 817
Bme18I GGWCC 1 cut(s) 360
BmgT120I GGNCC 1 cut(s) 360
BmiI GGNNCC 3 cut(s) 74, 361, 656
BmrFI CCNGG 2 cut(s) 761, 817
BmsI GCATC 2 cut(s) 296, 859
BpiI GAAGAC 1 cut(s) 1210
BplI GAGNNNNNCTC 2 cut(s) 921, 953
BpmI CTGGAG 1 cut(s) 782
BpuEI CTTGAG 1 cut(s) 339
BsaI GGTCTC 1 cut(s) 758
BsaJI CCNNGG 5 cut(s) 171, 450, 541, 815, 816
BsaWI WCCGGW 3 cut(s) 401, 514, 617
BsaXI ACNNNNNCTCC 2 cut(s) 57, 87
Bsc4I CCNNNNNNNGG 1 cut(s) 896
Bse1I ACTGG 1 cut(s) 656
Bse3DI GCAATG 2 cut(s) 837, 966
BseAI TCCGGA 1 cut(s) 401
BseBI CCWGG 2 cut(s) 761, 817
BseDI CCNNGG 5 cut(s) 171, 450, 541, 815, 816
BseGI GGATG 3 cut(s) 233, 874, 1131
BseLI CCNNNNNNNGG 1 cut(s) 896
BseMI GCAATG 2 cut(s) 837, 966
BseMII CTCAG 3 cut(s) 105, 113, 206
BseNI ACTGG 1 cut(s) 656
BseXI GCAGC 1 cut(s) 130
BsgI GTGCAG 1 cut(s) 48
Bsh1236I CGCG 1 cut(s) 543
BshFI GGCC 3 cut(s) 54, 170, 567
BsiSI CCGG 4 cut(s) 51, 402, 515, 618
BslFI GGGAC 2 cut(s) 373, 448
BslI CCNNNNNNNGG 1 cut(s) 896
BsmAI GTCTC 5 cut(s) 212, 758, 953, 1134, 1191
BsmFI GGGAC 2 cut(s) 373, 448
BsnI GGCC 3 cut(s) 54, 170, 567
Bso31I GGTCTC 1 cut(s) 758
Bsp1286I GDGCHC 1 cut(s) 75
Bsp13I TCCGGA 1 cut(s) 401
Bsp143I GATC 9 cut(s) 87, 214, 256, 546, 702, 726, 772, 1046, 1069
Bsp19I CCATGG 1 cut(s) 171
BspACI CCGC 4 cut(s) 341, 541, 543, 1015
BspANI GGCC 3 cut(s) 54, 170, 567
BspCNI CTCAG 3 cut(s) 106, 114, 205
BspEI TCCGGA 1 cut(s) 401
BspFNI CGCG 1 cut(s) 543
BspLI GGNNCC 3 cut(s) 74, 361, 656
BspMAI CTGCAG 1 cut(s) 253
BspPI GGATC 4 cut(s) 95, 251, 554, 1054
BspTNI GGTCTC 1 cut(s) 758
BsrDI GCAATG 2 cut(s) 837, 966
BsrI ACTGG 1 cut(s) 656
BssECI CCNNGG 5 cut(s) 171, 450, 541, 815, 816
BssMI GATC 9 cut(s) 87, 214, 256, 546, 702, 726, 772, 1046, 1069
BssT1I CCWWGG 2 cut(s) 171, 450
Bst2UI CCWGG 2 cut(s) 761, 817
Bst4CI ACNGT 6 cut(s) 369, 608, 646, 711, 1093, 1199
BstAPI GCANNNNNTGC 2 cut(s) 839, 869
BstC8I GCNNGC 1 cut(s) 343
BstDEI CTNAG 6 cut(s) 114, 122, 192, 378, 969, 1208
BstDSI CCRYGG 2 cut(s) 171, 541
BstF5I GGATG 3 cut(s) 233, 874, 1131
BstFNI CGCG 1 cut(s) 543
BstKTI GATC 9 cut(s) 90, 217, 259, 549, 705, 729, 775, 1049, 1072
BstMAI GTCTC 5 cut(s) 212, 758, 953, 1134, 1191
BstMBI GATC 9 cut(s) 87, 214, 256, 546, 702, 726, 772, 1046, 1069
BstMWI GCNNNNNNNGC 6 cut(s) 79, 167, 176, 839, 869, 965
BstNI CCWGG 2 cut(s) 761, 817
BstSCI CCNGG 2 cut(s) 759, 815
BstSFI CTRYAG 1 cut(s) 249
BstUI CGCG 1 cut(s) 543
BstV1I GCAGC 1 cut(s) 130
BstV2I GAAGAC 1 cut(s) 1210
BstX2I RGATCY 1 cut(s) 256
BstXI CCANNNNNNTGG 2 cut(s) 83, 457
BstYI RGATCY 1 cut(s) 256
BsuI GTATCC 1 cut(s) 767
BsuRI GGCC 3 cut(s) 54, 170, 567
BtgI CCRYGG 2 cut(s) 171, 541
BtgZI GCGATG 1 cut(s) 932
BtsCI GGATG 3 cut(s) 233, 874, 1131
BtsI GCAGTG 1 cut(s) 60
BtsIMutI CAGTG 4 cut(s) 60, 489, 642, 768
Cac8I GCNNGC 1 cut(s) 343
CaiI CAGNNNCTG 1 cut(s) 287
Cfr13I GGNCC 1 cut(s) 360
Cfr42I CCGCGG 1 cut(s) 544
CseI GACGC 1 cut(s) 341
Csp6I GTAC 2 cut(s) 15, 311
CspCI CAANNNNNGTGG 4 cut(s) 49, 84, 757, 792
CviAII CATG 5 cut(s) 172, 630, 802, 1150, 1214
CviQI GTAC 2 cut(s) 15, 311
DdeI CTNAG 6 cut(s) 114, 122, 192, 378, 969, 1208
DpnI GATC 9 cut(s) 89, 216, 258, 548, 704, 728, 774, 1048, 1071
DpnII GATC 9 cut(s) 87, 214, 256, 546, 702, 726, 772, 1046, 1069
EciI GGCGGA 1 cut(s) 1030
Eco130I CCWWGG 2 cut(s) 171, 450
Eco24I GRGCYC 1 cut(s) 75
Eco31I GGTCTC 1 cut(s) 758
Eco32I GATATC 1 cut(s) 25
Eco47I GGWCC 1 cut(s) 360
Eco57I CTGAAG 1 cut(s) 309
EcoRII CCWGG 2 cut(s) 759, 815
EcoRV GATATC 1 cut(s) 25
EcoT14I CCWWGG 2 cut(s) 171, 450
EcoT38I GRGCYC 1 cut(s) 75
ErhI CCWWGG 2 cut(s) 171, 450
FaeI CATG 5 cut(s) 175, 633, 805, 1153, 1217
FaqI GGGAC 2 cut(s) 373, 448
FatI CATG 5 cut(s) 171, 629, 801, 1149, 1213
FauI CCCGC 2 cut(s) 334, 536
FblI GTMKAC 1 cut(s) 648
Fnu4HI GCNGC 1 cut(s) 119
FokI GGATG 3 cut(s) 240, 881, 1138
FriOI GRGCYC 1 cut(s) 75
Fsp4HI GCNGC 1 cut(s) 119
FspBI CTAG 2 cut(s) 338, 500
GluI GCNGC 1 cut(s) 119
GsuI CTGGAG 1 cut(s) 782
HaeIII GGCC 3 cut(s) 54, 170, 567
HapII CCGG 4 cut(s) 51, 402, 515, 618
HgaI GACGC 1 cut(s) 341
Hin1II CATG 5 cut(s) 175, 633, 805, 1153, 1217
HindIII AAGCTT 1 cut(s) 380
HinfI GANTC 6 cut(s) 45, 107, 284, 511, 793, 886
HpaII CCGG 4 cut(s) 51, 402, 515, 618
HphI GGTGA 5 cut(s) 224, 799, 869, 965, 995
Hpy166II GTNNAC 2 cut(s) 388, 649
Hpy188I TCNGA 7 cut(s) 38, 92, 115, 239, 289, 430, 699
Hpy188III TCNNGA 7 cut(s) 281, 402, 508, 559, 751, 776, 890
Hpy8I GTNNAC 2 cut(s) 388, 649
Hpy99I CGWCG 2 cut(s) 38, 434
HpyAV CCTTC 2 cut(s) 160, 576
HpyCH4III ACNGT 6 cut(s) 369, 608, 646, 711, 1093, 1199
HpyCH4IV ACGT 1 cut(s) 852
HpyCH4V TGCA 7 cut(s) 65, 140, 251, 842, 863, 872, 1043
HpyF10VI GCNNNNNNNGC 6 cut(s) 79, 167, 176, 839, 869, 965
HpyF3I CTNAG 6 cut(s) 114, 122, 192, 378, 969, 1208
HpySE526I ACGT 1 cut(s) 852
Hsp92II CATG 5 cut(s) 175, 633, 805, 1153, 1217
Kpn2I TCCGGA 1 cut(s) 401
KspI CCGCGG 1 cut(s) 544
Kzo9I GATC 9 cut(s) 87, 214, 256, 546, 702, 726, 772, 1046, 1069
LmnI GCTCC 2 cut(s) 38, 78
Lsp1109I GCAGC 1 cut(s) 130
LweI GCATC 2 cut(s) 296, 859
MaeI CTAG 2 cut(s) 338, 500
MaeII ACGT 1 cut(s) 852
MaeIII GTNAC 1 cut(s) 632
MalI GATC 9 cut(s) 89, 216, 258, 548, 704, 728, 774, 1048, 1071
MboI GATC 9 cut(s) 87, 214, 256, 546, 702, 726, 772, 1046, 1069
MboII GAAGA 7 cut(s) 102, 302, 712, 1004, 1034, 1045, 1215
MflI RGATCY 1 cut(s) 256
MhlI GDGCHC 1 cut(s) 75
MluCI AATT 3 cut(s) 347, 395, 572
MlyI GAGTC 1 cut(s) 880
MmeI TCCRAC 2 cut(s) 16, 453
MroI TCCGGA 1 cut(s) 401
MseI TTAA 3 cut(s) 156, 444, 519
MspA1I CMGCKG 2 cut(s) 121, 543
MspI CCGG 4 cut(s) 51, 402, 515, 618
MspR9I CCNGG 2 cut(s) 761, 817
MvaI CCWGG 2 cut(s) 761, 817
MvnI CGCG 1 cut(s) 543
MwoI GCNNNNNNNGC 6 cut(s) 79, 167, 176, 839, 869, 965
NcoI CCATGG 1 cut(s) 171
NdeII GATC 9 cut(s) 87, 214, 256, 546, 702, 726, 772, 1046, 1069
NlaIII CATG 5 cut(s) 175, 633, 805, 1153, 1217
NlaIV GGNNCC 3 cut(s) 74, 361, 656
NmeAIII GCCGAG 1 cut(s) 893
NmuCI GTSAC 1 cut(s) 632
PasI CCCWGGG 1 cut(s) 816
PfeI GAWTC 5 cut(s) 45, 107, 284, 511, 793
PfoI TCCNGGA 1 cut(s) 759
PkrI GCNGC 1 cut(s) 120
PleI GAGTC 1 cut(s) 880
PpsI GAGTC 1 cut(s) 880
PsiI TTATAA 1 cut(s) 1076
Psp6I CCWGG 2 cut(s) 759, 815
PspGI CCWGG 2 cut(s) 759, 815
PspN4I GGNNCC 3 cut(s) 74, 361, 656
PspPI GGNCC 1 cut(s) 360
PstI CTGCAG 1 cut(s) 253
PstNI CAGNNNCTG 1 cut(s) 287
PsuI RGATCY 1 cut(s) 256
PvuII CAGCTG 1 cut(s) 121
RsaI GTAC 2 cut(s) 16, 312
RsaNI GTAC 2 cut(s) 15, 311
SacII CCGCGG 1 cut(s) 544
SaqAI TTAA 3 cut(s) 156, 444, 519
SatI GCNGC 1 cut(s) 119
Sau3AI GATC 9 cut(s) 87, 214, 256, 546, 702, 726, 772, 1046, 1069
Sau96I GGNCC 1 cut(s) 360
SchI GAGTC 1 cut(s) 880
ScrFI CCNGG 2 cut(s) 761, 817
SduI GDGCHC 1 cut(s) 75
SfaNI GCATC 2 cut(s) 296, 859
SfcI CTRYAG 1 cut(s) 249
Sfr303I CCGCGG 1 cut(s) 544
SgrBI CCGCGG 1 cut(s) 544
SinI GGWCC 1 cut(s) 360
SmlI CTYRAG 1 cut(s) 354
SmoI CTYRAG 1 cut(s) 354
Sse9I AATT 3 cut(s) 347, 395, 572
SsiI CCGC 4 cut(s) 341, 541, 543, 1015
SspMI CTAG 2 cut(s) 338, 500
StyD4I CCNGG 2 cut(s) 759, 815
StyI CCWWGG 2 cut(s) 171, 450
TaaI ACNGT 6 cut(s) 369, 608, 646, 711, 1093, 1199
TaiI ACGT 1 cut(s) 855
TaqI TCGA 4 cut(s) 304, 393, 808, 1049
TasI AATT 3 cut(s) 347, 395, 572
TfiI GAWTC 5 cut(s) 45, 107, 284, 511, 793
Tru1I TTAA 3 cut(s) 156, 444, 519
Tru9I TTAA 3 cut(s) 156, 444, 519
TscAI CASTG 4 cut(s) 67, 489, 649, 775
TseFI GTSAC 1 cut(s) 632
TseI GCWGC 1 cut(s) 118
Tsp45I GTSAC 1 cut(s) 632
TspDTI ATGAA 8 cut(s) 135, 410, 613, 806, 977, 992, 1090, 1138
TspGWI ACGGA 1 cut(s) 942
TspRI CASTG 4 cut(s) 67, 489, 649, 775
VpaK11BI GGWCC 1 cut(s) 360
XmiI GTMKAC 1 cut(s) 648
XspI CTAG 2 cut(s) 338, 500
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.