Rroxscaffold_7G00189720

Zeaxanthin epoxidase, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000348
Physical Location & Seq
Forward (+)
29541256 .. 29547287
6032 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00189720.1

Sequence Viewer

Length: 1113 bp
ATGGGGAGGATCGGTCCGTGCATTGCGGCGTTGTCATTTTCCGCCGGGTCGGGTCAACGAAGGCCGGGGTGCCTTCGATCCGGGTTTTTACGGGCAGCGGCGGCGGGGCGAAATGATGGCGAGAGGGCGGGCGGGGCGGGGCAGGGCGACTTCGGGAGGCCGGGGAATGTTGAGGAATGCAGAAAGTTTTCCGAGACATTGTATAAGTTTGTATATATTCAAGGAACATACTTGAAGCCACATGTAGCTATCCTGGATGGTATAACTATGGGACGTGGGGCTGGAATTTCACTCCCAGGAATGTTTCATGTGGTGACCGATAAAGCTGTTTTGGTTGGATGTGATGGAGTAAACTCAGTGGTTGCAAAATGGCTGGGCTTCAAGCCGCCGGTCTTTACAGGAAGATCTGCCATTCGAGGTTGTGCTGAGTTCAAGAACAGCCATGAGTTTGATCCCATGTTCATGCAGTACTTTGGGAATGGTGTTAGATCTGCTGTAGTTCCTTGTGATGATAAAAATGTTTACTGGGCCATCACTTGGTCTCCCTCGAGCCATGAGAAAGAGCTAGAGGAAAACCCAGCTCAGTTGAAGCGATATATGTTAAGCAAACTCGGAAAGGTATCAGATGAAGTACGGGCCGTTGTGGAAAACACCGATTTGGATGCTTTTATATCCTCTCCATTGAGATGTAGGCATCCTTGGGAACTTATTTGGGGAAATATTAGCAAAGGTAATGTATGTGTGGCTGGAGACGCGCTCCACCCCATGACCCCAGACATTGGACAAGGTGGCTGTGCTGCATTAGAGGACGGTGTTGTATTAGCAAGGTGTCTTGGTGAGGCTTTGTTGAAGAATCGGAGCCAAGAAATTAGAGATGAAGGTGAAGAAGGAAAAGTGGAATATAAAATGATTGAAAGAGGGTTGAATAAGTATGCCAGTGAGAGGAGATGGAGAAGTTTTGATCTTATTAGTACAGCTTATGTGGTTGGTTTTATACAGGAGGCTGATGGAAAAATAATGACTTTCTTGAGGGACAAGTTTTTCTCTCCAATCGTGGCCGGGTTGCTGTTGAAGAAGGCTGATTATGATTGTGGTAAGCTCAGAAGCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAD_binding_3

Protein Analysis

370

Amino Acids

40.55

Weight (kDa)

8.56

Isoelectric Point (pI)

38.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ECH_2 PF16113 56 - 110 2.1e-12 Enoyl-CoA hydratase/isomerase
FAD_binding_3 PF01494 111 - 290 2.3e-12 FAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000348)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G38540 AT5G05320
fragaria_vesca FvH4_1g12561 FvH4_1g12581 FvH4_2g15511 FvH4_2g15511 FvH4_2g15511 FvH4_2g15511 FvH4_2g15511 FvH4_2g15511 FvH4_2g15511 FvH4_2g15511 FvH4_2g15511 FvH4_3g33121 FvH4_3g33122 FvH4_4g08974 FvH4_4g09610 FvH4_7g17841
malus_domestica MD05G1123600.v1.1 MD05G1123700.v1.1
prunus_persica Prupe.8G167400_v2.0.a1 Prupe.8G167500_v2.0.a1 Prupe.8G167700_v2.0.a1 Prupe.8G167700_v2.0.a1
pyrus_communis pycom05g11780 pycom05g11790
rosa_chinensis RchiOBHm_Chr6g0278521 RchiOBHm_Chr6g0278531 RchiOBHm_Chr6g0278551 RchiOBHm_Chr6g0278591 RchiOBHm_Chr6g0278611 RchiOBHm_Chr6g0278671 RchiOBHm_Chr6g0278691 RchiOBHm_Chr6g0278701
rosa_laevigata RLG00000013200 RLG00000013201 RLG00000013203 RLG00000013205 RLG00000013206 RLG00000013210 RLG00000013211 RLG00000013213 RLG00000013216 RLG00000013217
rosa_multiflora Rmu_sc0000258.1_g000072 Rmu_sc0000258.1_g000073 Rmu_sc0000258.1_g000082 Rmu_sc0000258.1_g000083 Rmu_sc0000258.1_g000090 Rmu_sc0000258.1_g000094 Rmu_sc0000258.1_g000096 Rmu_sc0002777.1_g000006 Rmu_sc0002777.1_g000011 Rmu_sc0002777.1_g000019 Rmu_sc0006193.1_g000001 Rmu_sc0034228.1_g000001 Rmu_ssc0000042.1_g000052 Rmu_ssc0000144.1_g000014
rosa_roxburghii Rroxscaffold_7G00189700 Rroxscaffold_7G00189710 Rroxscaffold_7G00189720 Rroxscaffold_7G00189730 Rroxscaffold_7G00189770 Rroxscaffold_7G00189800 Rroxscaffold_7G00189820 Rroxscaffold_7G00189960 Rroxscaffold_7G00190070 Rroxscaffold_7G00190090 Rroxscaffold_7G00190140 Rroxscaffold_7G00190160 Rroxscaffold_7G00190170 Rroxscaffold_7G00190270 Rroxscaffold_7G00190280 Rroxscaffold_7G00190300 Rroxscaffold_7G00190310
rosa_rugosa Rorug02G0222700 Rorug06G0117300 Rorug06G0117400 Rorug06G0117700 Rorug06G0117900 Rorug06G0118000 Rorug06G0118100 Rorug06G0118200
rosa_samantha Rh1BG066900 Rh2BG292300 Rh6BG231700 Rh6BG231800 Rh6BG232000 Rh6BG232300 Rh6BG232500 Rh6CG234100 Rh6CG234200 Rh6CG234300 Rh6CG234700 Rh6DG225400 Rh6DG225500 Rh6DG225800 Rh6DG226000 Rh6DG226300
rosa_wichuraiana Rw0G003090 Rw0G003690 Rw0G021770 Rw4G037090 Rw6G019870 Rw6G019890 Rw6G019900 Rw6G019910 Rw6G019920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 69
AccB7I CCANNNNNTGG 2 cut(s) 537, 779
AccII CGCG 1 cut(s) 755
AciI CCGC 9 cut(s) 26, 42, 98, 101, 104, 128, 132, 137, 386
AclWI GGATC 3 cut(s) 17, 72, 446
AcoI YGGCCR 1 cut(s) 1056
AcsI RAATTY 1 cut(s) 285
AfaI GTAC 3 cut(s) 470, 633, 973
AfiI CCNNNNNNNGG 3 cut(s) 537, 779, 942
AflIII ACRYGT 1 cut(s) 241
AgsI TTSAA 9 cut(s) 221, 235, 382, 433, 589, 850, 914, 925, 1072
AjiI CACGTC 1 cut(s) 275
AjnI CCWGG 2 cut(s) 252, 295
AluBI AGCT 7 cut(s) 248, 326, 565, 581, 977, 1099, 1107
AluI AGCT 7 cut(s) 248, 326, 565, 581, 977, 1099, 1107
Alw26I GTCTC 3 cut(s) 188, 546, 744
AlwI GGATC 3 cut(s) 17, 72, 446
Ama87I CYCGRG 1 cut(s) 547
AoxI GGCC 5 cut(s) 62, 158, 528, 636, 1056
ApeKI GCWGC 2 cut(s) 95, 797
ApoI RAATTY 1 cut(s) 285
Asp700I GAANNNNTTC 1 cut(s) 187
AspLEI GCGC 1 cut(s) 757
AspS9I GGNCC 3 cut(s) 14, 528, 636
AsuC2I CCSGG 5 cut(s) 46, 66, 82, 162, 1060
AsuHPI GGTGA 3 cut(s) 325, 848, 893
AvaI CYCGRG 1 cut(s) 547
AvaII GGWCC 1 cut(s) 14
BanI GGYRCC 1 cut(s) 69
BbvI GCAGC 2 cut(s) 107, 784
BccI CCATC 6 cut(s) 110, 251, 338, 539, 942, 1001
BceAI ACGGC 1 cut(s) 623
BcgI CGANNNNNNTGC 2 cut(s) 644, 678
BciT130I CCWGG 2 cut(s) 254, 297
BcnI CCSGG 5 cut(s) 46, 66, 82, 162, 1060
BcoDI GTCTC 3 cut(s) 188, 546, 744
BfaI CTAG 1 cut(s) 566
BfmI CTRYAG 1 cut(s) 495
BglII AGATCT 2 cut(s) 404, 488
BisI GCNGC 6 cut(s) 27, 96, 99, 102, 386, 798
BlsI GCNGC 6 cut(s) 28, 97, 100, 103, 387, 799
BmcAI AGTACT 1 cut(s) 470
Bme1390I CCNGG 7 cut(s) 46, 66, 82, 162, 254, 297, 1060
Bme18I GGWCC 1 cut(s) 14
BmeT110I CYCGRG 1 cut(s) 547
BmgBI CACGTC 1 cut(s) 275
BmgT120I GGNCC 3 cut(s) 14, 528, 636
BmiI GGNNCC 2 cut(s) 71, 860
BmrFI CCNGG 7 cut(s) 46, 66, 82, 162, 254, 297, 1060
BmrI ACTGGG 1 cut(s) 535
BmsI GCATC 2 cut(s) 652, 703
BmuI ACTGGG 1 cut(s) 535
BplI GAGNNNNNCTC 2 cut(s) 741, 773
BpmI CTGGAG 1 cut(s) 768
BpuEI CTTGAG 1 cut(s) 1048
BpuMI CCSGG 5 cut(s) 46, 66, 82, 162, 1060
BsaI GGTCTC 1 cut(s) 546
BsaJI CCNNGG 4 cut(s) 65, 161, 295, 698
BsaXI ACNNNNNCTCC 3 cut(s) 28, 526, 556
Bsc4I CCNNNNNNNGG 3 cut(s) 537, 779, 942
Bse118I RCCGGY 1 cut(s) 388
Bse1I ACTGG 2 cut(s) 530, 936
Bse3DI GCAATG 1 cut(s) 21
BseBI CCWGG 2 cut(s) 254, 297
BseDI CCNNGG 4 cut(s) 65, 161, 295, 698
BseGI GGATG 4 cut(s) 262, 344, 667, 694
BseLI CCNNNNNNNGG 3 cut(s) 537, 779, 942
BseMI GCAATG 1 cut(s) 21
BseMII CTCAG 3 cut(s) 369, 417, 596
BseNI ACTGG 2 cut(s) 530, 936
BseRI GAGGAG 1 cut(s) 958
BseXI GCAGC 2 cut(s) 107, 784
BseYI CCCAGC 2 cut(s) 373, 577
Bsh1236I CGCG 1 cut(s) 755
BshFI GGCC 5 cut(s) 64, 160, 530, 638, 1058
BshNI GGYRCC 1 cut(s) 69
BsiHKCI CYCGRG 1 cut(s) 547
BsiSI CCGG 6 cut(s) 45, 65, 81, 161, 389, 1059
BslFI GGGAC 2 cut(s) 285, 1046
BslI CCNNNNNNNGG 3 cut(s) 537, 779, 942
BsmAI GTCTC 3 cut(s) 188, 546, 744
BsmBI CGTCTC 1 cut(s) 744
BsmFI GGGAC 2 cut(s) 285, 1046
BsmI GAATGC 1 cut(s) 182
BsnI GGCC 5 cut(s) 64, 160, 530, 638, 1058
Bso31I GGTCTC 1 cut(s) 546
BsoBI CYCGRG 1 cut(s) 547
Bsp143I GATC 6 cut(s) 9, 77, 404, 451, 488, 961
BspACI CCGC 9 cut(s) 26, 42, 98, 101, 104, 128, 132, 137, 386
BspANI GGCC 5 cut(s) 64, 160, 530, 638, 1058
BspCNI CTCAG 4 cut(s) 368, 418, 595, 1113
BspFNI CGCG 1 cut(s) 755
BspLI GGNNCC 2 cut(s) 71, 860
BspPI GGATC 3 cut(s) 17, 72, 446
BspT107I GGYRCC 1 cut(s) 69
BspTNI GGTCTC 1 cut(s) 546
BsrDI GCAATG 1 cut(s) 21
BsrFI RCCGGY 1 cut(s) 388
BsrI ACTGG 2 cut(s) 530, 936
BssAI RCCGGY 1 cut(s) 388
BssECI CCNNGG 4 cut(s) 65, 161, 295, 698
BssMI GATC 6 cut(s) 9, 77, 404, 451, 488, 961
BssT1I CCWWGG 1 cut(s) 698
Bst2UI CCWGG 2 cut(s) 254, 297
Bst4CI ACNGT 1 cut(s) 812
BstC8I GCNNGC 1 cut(s) 130
BstDEI CTNAG 4 cut(s) 355, 426, 582, 1100
BstEII GGTNACC 1 cut(s) 313
BstF5I GGATG 4 cut(s) 262, 344, 667, 694
BstFNI CGCG 1 cut(s) 755
BstHHI GCGC 1 cut(s) 757
BstKTI GATC 6 cut(s) 12, 80, 407, 454, 491, 964
BstMAI GTCTC 3 cut(s) 188, 546, 744
BstMBI GATC 6 cut(s) 9, 77, 404, 451, 488, 961
BstMWI GCNNNNNNNGC 3 cut(s) 101, 134, 752
BstNI CCWGG 2 cut(s) 254, 297
BstNSI RCATGY 1 cut(s) 245
BstPI GGTNACC 1 cut(s) 313
BstSCI CCNGG 7 cut(s) 44, 64, 80, 160, 252, 295, 1058
BstSFI CTRYAG 1 cut(s) 495
BstUI CGCG 1 cut(s) 755
BstV1I GCAGC 2 cut(s) 107, 784
BstX2I RGATCY 2 cut(s) 404, 488
BstYI RGATCY 2 cut(s) 404, 488
BsuRI GGCC 5 cut(s) 64, 160, 530, 638, 1058
BtrI CACGTC 1 cut(s) 275
BtsCI GGATG 4 cut(s) 262, 344, 667, 694
BtsIMutI CAGTG 2 cut(s) 363, 943
Cac8I GCNNGC 1 cut(s) 130
CfoI GCGC 1 cut(s) 757
Cfr10I RCCGGY 1 cut(s) 388
Cfr13I GGNCC 3 cut(s) 14, 528, 636
CpoI CGGWCCG 1 cut(s) 14
CseI GACGC 1 cut(s) 761
Csp6I GTAC 3 cut(s) 469, 632, 972
CspI CGGWCCG 1 cut(s) 14
CviAII CATG 7 cut(s) 242, 308, 443, 457, 463, 554, 766
CviQI GTAC 3 cut(s) 469, 632, 972
DdeI CTNAG 4 cut(s) 355, 426, 582, 1100
DpnI GATC 6 cut(s) 11, 79, 406, 453, 490, 963
DpnII GATC 6 cut(s) 9, 77, 404, 451, 488, 961
EaeI YGGCCR 1 cut(s) 1056
EciI GGCGGA 1 cut(s) 31
Eco130I CCWWGG 1 cut(s) 698
Eco31I GGTCTC 1 cut(s) 546
Eco47I GGWCC 1 cut(s) 14
Eco88I CYCGRG 1 cut(s) 547
Eco91I GGTNACC 1 cut(s) 313
EcoO65I GGTNACC 1 cut(s) 313
EcoRII CCWGG 2 cut(s) 252, 295
EcoT14I CCWWGG 1 cut(s) 698
ErhI CCWWGG 1 cut(s) 698
Esp3I CGTCTC 1 cut(s) 744
FaeI CATG 7 cut(s) 245, 311, 446, 460, 466, 557, 769
FaqI GGGAC 2 cut(s) 285, 1046
FatI CATG 7 cut(s) 241, 307, 442, 456, 462, 553, 765
FauI CCCGC 4 cut(s) 97, 121, 125, 130
Fnu4HI GCNGC 6 cut(s) 27, 96, 99, 102, 386, 798
FokI GGATG 4 cut(s) 269, 351, 674, 681
Fsp4HI GCNGC 6 cut(s) 27, 96, 99, 102, 386, 798
FspBI CTAG 1 cut(s) 566
GlaI GCGC 1 cut(s) 756
GluI GCNGC 6 cut(s) 27, 96, 99, 102, 386, 798
GsaI CCCAGC 2 cut(s) 377, 581
GsuI CTGGAG 1 cut(s) 768
HaeIII GGCC 5 cut(s) 64, 160, 530, 638, 1058
HapII CCGG 6 cut(s) 45, 65, 81, 161, 389, 1059
HgaI GACGC 1 cut(s) 761
HhaI GCGC 1 cut(s) 757
Hin1II CATG 7 cut(s) 245, 311, 446, 460, 466, 557, 769
Hin6I GCGC 1 cut(s) 755
HinP1I GCGC 1 cut(s) 755
HincII GTYRAC 1 cut(s) 56
HindII GTYRAC 1 cut(s) 56
HindIII AAGCTT 1 cut(s) 1105
HinfI GANTC 1 cut(s) 853
HpaII CCGG 6 cut(s) 45, 65, 81, 161, 389, 1059
HphI GGTGA 3 cut(s) 325, 848, 893
Hpy166II GTNNAC 3 cut(s) 56, 352, 523
Hpy188I TCNGA 5 cut(s) 193, 614, 625, 858, 1103
Hpy188III TCNNGA 3 cut(s) 154, 433, 1027
Hpy8I GTNNAC 3 cut(s) 56, 352, 523
HpyAV CCTTC 5 cut(s) 54, 83, 872, 881, 1069
HpyCH4III ACNGT 1 cut(s) 812
HpyCH4IV ACGT 1 cut(s) 274
HpyCH4V TGCA 5 cut(s) 21, 180, 365, 466, 800
HpyF10VI GCNNNNNNNGC 3 cut(s) 101, 134, 752
HpyF3I CTNAG 4 cut(s) 355, 426, 582, 1100
HpySE526I ACGT 1 cut(s) 274
Hsp92II CATG 7 cut(s) 245, 311, 446, 460, 466, 557, 769
HspAI GCGC 1 cut(s) 755
Kzo9I GATC 6 cut(s) 9, 77, 404, 451, 488, 961
LmnI GCTCC 2 cut(s) 762, 858
Lsp1109I GCAGC 2 cut(s) 107, 784
LweI GCATC 2 cut(s) 652, 703
MaeI CTAG 1 cut(s) 566
MaeII ACGT 1 cut(s) 274
MaeIII GTNAC 1 cut(s) 313
MalI GATC 6 cut(s) 11, 79, 406, 453, 490, 963
MboI GATC 6 cut(s) 9, 77, 404, 451, 488, 961
MboII GAAGA 4 cut(s) 414, 862, 896, 1084
MflI RGATCY 2 cut(s) 404, 488
MluCI AATT 2 cut(s) 285, 867
MmeI TCCRAC 1 cut(s) 316
MroXI GAANNNNTTC 1 cut(s) 187
MseI TTAA 2 cut(s) 602, 1111
MslI CAYNNNNRTG 2 cut(s) 461, 685
MspA1I CMGCKG 1 cut(s) 98
MspI CCGG 6 cut(s) 45, 65, 81, 161, 389, 1059
MspR9I CCNGG 7 cut(s) 46, 66, 82, 162, 254, 297, 1060
Mva1269I GAATGC 1 cut(s) 182
MvaI CCWGG 2 cut(s) 254, 297
MvnI CGCG 1 cut(s) 755
MwoI GCNNNNNNNGC 3 cut(s) 101, 134, 752
NciI CCSGG 5 cut(s) 46, 66, 82, 162, 1060
NdeII GATC 6 cut(s) 9, 77, 404, 451, 488, 961
NlaIII CATG 7 cut(s) 245, 311, 446, 460, 466, 557, 769
NlaIV GGNNCC 2 cut(s) 71, 860
NmuCI GTSAC 1 cut(s) 313
NspI RCATGY 1 cut(s) 245
PaeR7I CTCGAG 1 cut(s) 547
PciI ACATGT 1 cut(s) 241
PctI GAATGC 1 cut(s) 182
PdmI GAANNNNTTC 1 cut(s) 187
PfeI GAWTC 1 cut(s) 853
PflMI CCANNNNNTGG 2 cut(s) 537, 779
PfoI TCCNGGA 1 cut(s) 252
PkrI GCNGC 6 cut(s) 28, 97, 100, 103, 387, 799
PscI ACATGT 1 cut(s) 241
Psp6I CCWGG 2 cut(s) 252, 295
PspEI GGTNACC 1 cut(s) 313
PspFI CCCAGC 2 cut(s) 373, 577
PspGI CCWGG 2 cut(s) 252, 295
PspN4I GGNNCC 2 cut(s) 71, 860
PspPI GGNCC 3 cut(s) 14, 528, 636
PspXI VCTCGAGB 1 cut(s) 547
PsuI RGATCY 2 cut(s) 404, 488
RsaI GTAC 3 cut(s) 470, 633, 973
RsaNI GTAC 3 cut(s) 469, 632, 972
RseI CAYNNNNRTG 2 cut(s) 461, 685
Rsr2I CGGWCCG 1 cut(s) 14
RsrII CGGWCCG 1 cut(s) 14
SaqAI TTAA 2 cut(s) 602, 1111
SatI GCNGC 6 cut(s) 27, 96, 99, 102, 386, 798
Sau3AI GATC 6 cut(s) 9, 77, 404, 451, 488, 961
Sau96I GGNCC 3 cut(s) 14, 528, 636
ScaI AGTACT 1 cut(s) 470
ScrFI CCNGG 7 cut(s) 46, 66, 82, 162, 254, 297, 1060
SfaNI GCATC 2 cut(s) 652, 703
SfcI CTRYAG 1 cut(s) 495
Sfr274I CTCGAG 1 cut(s) 547
SinI GGWCC 1 cut(s) 14
SlaI CTCGAG 1 cut(s) 547
SmiMI CAYNNNNRTG 2 cut(s) 461, 685
SmlI CTYRAG 2 cut(s) 547, 1027
SmoI CTYRAG 2 cut(s) 547, 1027
Sse9I AATT 2 cut(s) 285, 867
SsiI CCGC 9 cut(s) 26, 42, 98, 101, 104, 128, 132, 137, 386
SspI AATATT 1 cut(s) 721
SspMI CTAG 1 cut(s) 566
StyD4I CCNGG 7 cut(s) 44, 64, 80, 160, 252, 295, 1058
StyI CCWWGG 1 cut(s) 698
TaaI ACNGT 1 cut(s) 812
TaiI ACGT 1 cut(s) 277
TaqI TCGA 3 cut(s) 76, 415, 548
TaqII GACCGA 1 cut(s) 332
TasI AATT 2 cut(s) 285, 867
TatI WGTACW 2 cut(s) 468, 971
TauI GCSGC 4 cut(s) 29, 101, 104, 388
TfiI GAWTC 1 cut(s) 853
Tru1I TTAA 2 cut(s) 602, 1111
Tru9I TTAA 2 cut(s) 602, 1111
TscAI CASTG 2 cut(s) 363, 943
TseFI GTSAC 1 cut(s) 313
TseI GCWGC 2 cut(s) 95, 797
Tsp45I GTSAC 1 cut(s) 313
TspDTI ATGAA 4 cut(s) 296, 451, 642, 891
TspGWI ACGGA 1 cut(s) 6
TspRI CASTG 2 cut(s) 363, 943
Van91I CCANNNNNTGG 2 cut(s) 537, 779
VpaK11BI GGWCC 1 cut(s) 14
XapI RAATTY 1 cut(s) 285
XceI RCATGY 1 cut(s) 245
XhoI CTCGAG 1 cut(s) 547
XmnI GAANNNNTTC 1 cut(s) 187
XspI CTAG 1 cut(s) 566
ZrmI AGTACT 1 cut(s) 470
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.