Rroxscaffold_7G00190070

Zeaxanthin epoxidase, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000348
Physical Location & Seq
Forward (+)
30089168 .. 30096534
7367 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00190070.1

Sequence Viewer

Length: 1347 bp
ATGCAAGTAGTAGAGGACGTTGTGATTGTGGGAGCTGGAATTTCTGGCCTCACAACCGCCCTGGGACTTCACAGGCTGGGCATTAGGAGCTTAGTGCTGGAATCGTTTGATAGCTTGAGGATAACAGGGTTTGCACTCACAACATGGACTAATGCATGGAAGGCTTTAGATGCCATTGGTGTTGGTGATCATTTACGGCAGCAACACCTGACTCTTCTTGGGAATGTGGTTTTCTCGAGAATTTCAGGGCTTCAGATGTCTGAAATGTCACTTAAGGGAAAAGAAAACAAAGATGATCAAGAGGACGACGAGGCCAACGAGCATGTACGCGACGGAGAATATTACGGACATTTCATCGGTGGTGGTGATCGGCGTAGGGGTTGTGGTGGTGATTGTGGAGACCATGAAATTCGTTGTGTGAAAAGGAAGTCGCTGTTAGAAGCCCTTGCGAGTGAACTTCCTAGTGGCACCATTAGGTTCTCTTCAAAGGTTGTTTCCATTGAGGAATCAGGCTACTTTAAGCTGGTGCATCTTGCCGACGGAACCATCCTCAAAGCCAAGGTTTTGGTTGGATGTGATGGAGTAAACTCAGTGGTTGCAAAATGGCTGGGCTTCAAGCCGCCGGTCTTTACAGGAAGATCTGCTGTTCGAGGTAGTGTTGAGTTCAAGAGCAGCCATGATTTTGATCCCATGTTCATGCAGTACTTTGGGAATGGTGTTAGATCTGGTGTAGTTCCATGTGATGATAAAAATGTTTACTGGTACTTCACTTGGTCTCCCTCCAGCCAAGAGAAAGAGCTAGAAGAAAACCCAGCTCAGTTGAAGCAATATGTGTTAAGCAAGCTCGGAAAAGTATCAGATGAAGTAAGGGATGTTGTGGAAAACACTGATTTGGATGCTTTTATATTCTCTCCATTGAGATATAGGCATCCTTGGGAACTTCTTTGGGGAAATATTAGCAAAGGTACTGTATGTGTAGCTGGAGATGCGCTCCACCCCATGACCCCAGACATTGGACAAGGCGGCTGTGCTGCATTAGAGGATGGTGTTGTATTAGCAAGGTGTCTCGGTGAGGCCTTGTTGAAGAATCGGAGCCAAGAAATTAGAGATGAAGGTGAAGAAGGAAAAGTGGAATGTAAAATGATTGAAAGAGGGTTGAATAAGTATGCCAGTGAGAGGAAATGGAGAAGTTTTGATCTTATTAGTACAGCTTATGTGGTTGGTTTTATACAGGAGGCTGATGGAAAAATAATGACTTTCTTGAGGGACAAGTTTTTCTCTCCAATCCTGGCCGGGTTGCGGTTGAAGAAGGCTGATTGTGATTGTGGTAAGCTCAGAAGCTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAD_binding_3

Protein Analysis

448

Amino Acids

49.53

Weight (kDa)

6.0

Isoelectric Point (pI)

39.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_3 PF01494 6 - 368 2.1e-26 FAD binding domain
DAO PF01266 6 - 198 3.9e-06 FAD dependent oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000348)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G38540 AT5G05320
fragaria_vesca FvH4_1g12561 FvH4_1g12581 FvH4_2g15511 FvH4_2g15511 FvH4_2g15511 FvH4_2g15511 FvH4_2g15511 FvH4_2g15511 FvH4_2g15511 FvH4_2g15511 FvH4_2g15511 FvH4_3g33121 FvH4_3g33122 FvH4_4g08974 FvH4_4g09610 FvH4_7g17841
malus_domestica MD05G1123600.v1.1 MD05G1123700.v1.1
prunus_persica Prupe.8G167400_v2.0.a1 Prupe.8G167500_v2.0.a1 Prupe.8G167700_v2.0.a1 Prupe.8G167700_v2.0.a1
pyrus_communis pycom05g11780 pycom05g11790
rosa_chinensis RchiOBHm_Chr6g0278521 RchiOBHm_Chr6g0278531 RchiOBHm_Chr6g0278551 RchiOBHm_Chr6g0278591 RchiOBHm_Chr6g0278611 RchiOBHm_Chr6g0278671 RchiOBHm_Chr6g0278691 RchiOBHm_Chr6g0278701
rosa_laevigata RLG00000013200 RLG00000013201 RLG00000013203 RLG00000013205 RLG00000013206 RLG00000013210 RLG00000013211 RLG00000013213 RLG00000013216 RLG00000013217
rosa_multiflora Rmu_sc0000258.1_g000072 Rmu_sc0000258.1_g000073 Rmu_sc0000258.1_g000082 Rmu_sc0000258.1_g000083 Rmu_sc0000258.1_g000090 Rmu_sc0000258.1_g000094 Rmu_sc0000258.1_g000096 Rmu_sc0002777.1_g000006 Rmu_sc0002777.1_g000011 Rmu_sc0002777.1_g000019 Rmu_sc0006193.1_g000001 Rmu_sc0034228.1_g000001 Rmu_ssc0000042.1_g000052 Rmu_ssc0000144.1_g000014
rosa_roxburghii Rroxscaffold_7G00189700 Rroxscaffold_7G00189710 Rroxscaffold_7G00189720 Rroxscaffold_7G00189730 Rroxscaffold_7G00189770 Rroxscaffold_7G00189800 Rroxscaffold_7G00189820 Rroxscaffold_7G00189960 Rroxscaffold_7G00190070 Rroxscaffold_7G00190090 Rroxscaffold_7G00190140 Rroxscaffold_7G00190160 Rroxscaffold_7G00190170 Rroxscaffold_7G00190270 Rroxscaffold_7G00190280 Rroxscaffold_7G00190300 Rroxscaffold_7G00190310
rosa_rugosa Rorug02G0222700 Rorug06G0117300 Rorug06G0117400 Rorug06G0117700 Rorug06G0117900 Rorug06G0118000 Rorug06G0118100 Rorug06G0118200
rosa_samantha Rh1BG066900 Rh2BG292300 Rh6BG231700 Rh6BG231800 Rh6BG232000 Rh6BG232300 Rh6BG232500 Rh6CG234100 Rh6CG234200 Rh6CG234300 Rh6CG234700 Rh6DG225400 Rh6DG225500 Rh6DG225800 Rh6DG226000 Rh6DG226300
rosa_wichuraiana Rw0G003090 Rw0G003690 Rw0G021770 Rw4G037090 Rw6G019870 Rw6G019890 Rw6G019900 Rw6G019910 Rw6G019920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 467
AccB7I CCANNNNNTGG 1 cut(s) 1013
AccII CGCG 1 cut(s) 330
AciI CCGC 4 cut(s) 57, 620, 1023, 1300
AclWI GGATC 1 cut(s) 680
AcoI YGGCCR 1 cut(s) 1290
AcsI RAATTY 3 cut(s) 39, 240, 408
AcuI CTGAAG 1 cut(s) 236
AfaI GTAC 5 cut(s) 327, 704, 764, 967, 1207
AfiI CCNNNNNNNGG 3 cut(s) 1013, 1176, 1299
AflII CTTAAG 1 cut(s) 272
AgsI TTSAA 8 cut(s) 486, 616, 667, 823, 1084, 1148, 1159, 1306
AjnI CCWGG 2 cut(s) 60, 1287
Alw26I GTCTC 3 cut(s) 393, 780, 1070
AlwI GGATC 1 cut(s) 680
Ama87I CYCGRG 1 cut(s) 235
AoxI GGCC 4 cut(s) 46, 312, 1074, 1290
ApeKI GCWGC 3 cut(s) 199, 672, 1031
ApoI RAATTY 3 cut(s) 39, 240, 408
AspLEI GCGC 1 cut(s) 991
AsuC2I CCSGG 1 cut(s) 1294
AsuHPI GGTGA 5 cut(s) 197, 377, 401, 1082, 1127
AvaI CYCGRG 1 cut(s) 235
BanI GGYRCC 1 cut(s) 467
BbvI GCAGC 3 cut(s) 211, 684, 1018
BccI CCATC 4 cut(s) 554, 572, 1037, 1235
BceAI ACGGC 1 cut(s) 212
BciT130I CCWGG 2 cut(s) 62, 1289
BclI TGATCA 2 cut(s) 187, 295
BcnI CCSGG 1 cut(s) 1294
BcoDI GTCTC 3 cut(s) 393, 780, 1070
BfaI CTAG 2 cut(s) 462, 800
BfrI CTTAAG 1 cut(s) 272
BglII AGATCT 2 cut(s) 638, 722
BisI GCNGC 5 cut(s) 200, 620, 673, 1024, 1032
BlsI GCNGC 5 cut(s) 201, 621, 674, 1025, 1033
BmcAI AGTACT 1 cut(s) 704
Bme1390I CCNGG 3 cut(s) 62, 1289, 1294
BmeT110I CYCGRG 1 cut(s) 235
BmiI GGNNCC 3 cut(s) 469, 544, 1094
BmrFI CCNGG 3 cut(s) 62, 1289, 1294
BmsI GCATC 5 cut(s) 160, 538, 886, 937, 976
BplI GAGNNNNNCTC 2 cut(s) 975, 1007
BpmI CTGGAG 2 cut(s) 766, 1002
BpuEI CTTGAG 2 cut(s) 136, 1282
BpuMI CCSGG 1 cut(s) 1294
BsaBI GATNNNNATC 1 cut(s) 684
BsaI GGTCTC 2 cut(s) 393, 780
BsaJI CCNNGG 4 cut(s) 60, 61, 558, 932
BsaXI ACNNNNNCTCC 2 cut(s) 760, 790
Bsc4I CCNNNNNNNGG 3 cut(s) 1013, 1176, 1299
Bse118I RCCGGY 1 cut(s) 622
Bse1I ACTGG 2 cut(s) 764, 1170
Bse8I GATNNNNATC 1 cut(s) 684
BseBI CCWGG 2 cut(s) 62, 1289
BseDI CCNNGG 4 cut(s) 60, 61, 558, 932
BseGI GGATG 6 cut(s) 546, 578, 877, 901, 928, 1048
BseJI GATNNNNATC 1 cut(s) 684
BseLI CCNNNNNNNGG 3 cut(s) 1013, 1176, 1299
BseMII CTCAG 2 cut(s) 603, 830
BseNI ACTGG 2 cut(s) 764, 1170
BseXI GCAGC 3 cut(s) 211, 684, 1018
BseYI CCCAGC 3 cut(s) 76, 607, 811
Bsh1236I CGCG 1 cut(s) 330
BshFI GGCC 4 cut(s) 48, 314, 1076, 1292
BshNI GGYRCC 1 cut(s) 467
BsiHKCI CYCGRG 1 cut(s) 235
BsiSI CCGG 2 cut(s) 623, 1293
BslFI GGGAC 2 cut(s) 78, 1280
BslI CCNNNNNNNGG 3 cut(s) 1013, 1176, 1299
BsmAI GTCTC 3 cut(s) 393, 780, 1070
BsmFI GGGAC 2 cut(s) 78, 1280
BsnI GGCC 4 cut(s) 48, 314, 1076, 1292
Bso31I GGTCTC 2 cut(s) 393, 780
BsoBI CYCGRG 1 cut(s) 235
Bsp143I GATC 7 cut(s) 187, 295, 367, 638, 685, 722, 1195
BspACI CCGC 4 cut(s) 57, 620, 1023, 1300
BspANI GGCC 4 cut(s) 48, 314, 1076, 1292
BspCNI CTCAG 3 cut(s) 602, 829, 1347
BspFNI CGCG 1 cut(s) 330
BspLI GGNNCC 3 cut(s) 469, 544, 1094
BspPI GGATC 1 cut(s) 680
BspT107I GGYRCC 1 cut(s) 467
BspTI CTTAAG 1 cut(s) 272
BspTNI GGTCTC 2 cut(s) 393, 780
BsrFI RCCGGY 1 cut(s) 622
BsrI ACTGG 2 cut(s) 764, 1170
BssAI RCCGGY 1 cut(s) 622
BssECI CCNNGG 4 cut(s) 60, 61, 558, 932
BssMI GATC 7 cut(s) 187, 295, 367, 638, 685, 722, 1195
BssT1I CCWWGG 2 cut(s) 558, 932
Bst2UI CCWGG 2 cut(s) 62, 1289
Bst4CI ACNGT 1 cut(s) 970
Bst6I CTCTTC 2 cut(s) 219, 487
BstAFI CTTAAG 1 cut(s) 272
BstC8I GCNNGC 1 cut(s) 842
BstDEI CTNAG 4 cut(s) 91, 589, 816, 1334
BstF5I GGATG 6 cut(s) 546, 578, 877, 901, 928, 1048
BstFNI CGCG 1 cut(s) 330
BstHHI GCGC 1 cut(s) 991
BstKTI GATC 7 cut(s) 190, 298, 370, 641, 688, 725, 1198
BstMAI GTCTC 3 cut(s) 393, 780, 1070
BstMBI GATC 7 cut(s) 187, 295, 367, 638, 685, 722, 1195
BstMWI GCNNNNNNNGC 4 cut(s) 87, 161, 170, 986
BstNI CCWGG 2 cut(s) 62, 1289
BstNSI RCATGY 1 cut(s) 326
BstSCI CCNGG 3 cut(s) 60, 1287, 1292
BstUI CGCG 1 cut(s) 330
BstV1I GCAGC 3 cut(s) 211, 684, 1018
BstX2I RGATCY 2 cut(s) 638, 722
BstXI CCANNNNNNTGG 1 cut(s) 565
BstYI RGATCY 2 cut(s) 638, 722
BsuRI GGCC 4 cut(s) 48, 314, 1076, 1292
BtsCI GGATG 6 cut(s) 546, 578, 877, 901, 928, 1048
BtsIMutI CAGTG 3 cut(s) 597, 885, 1177
Cac8I GCNNGC 1 cut(s) 842
CfoI GCGC 1 cut(s) 991
Cfr10I RCCGGY 1 cut(s) 622
Csp6I GTAC 5 cut(s) 326, 703, 763, 966, 1206
CviAII CATG 9 cut(s) 144, 156, 323, 404, 677, 691, 697, 738, 1000
CviQI GTAC 5 cut(s) 326, 703, 763, 966, 1206
DdeI CTNAG 4 cut(s) 91, 589, 816, 1334
DpnI GATC 7 cut(s) 189, 297, 369, 640, 687, 724, 1197
DpnII GATC 7 cut(s) 187, 295, 367, 638, 685, 722, 1195
EaeI YGGCCR 1 cut(s) 1290
Eam1104I CTCTTC 2 cut(s) 219, 487
EarI CTCTTC 2 cut(s) 219, 487
Eco130I CCWWGG 2 cut(s) 558, 932
Eco147I AGGCCT 1 cut(s) 1076
Eco31I GGTCTC 2 cut(s) 393, 780
Eco57I CTGAAG 1 cut(s) 236
Eco88I CYCGRG 1 cut(s) 235
EcoRII CCWGG 2 cut(s) 60, 1287
EcoT14I CCWWGG 2 cut(s) 558, 932
EcoT22I ATGCAT 1 cut(s) 157
ErhI CCWWGG 2 cut(s) 558, 932
FaeI CATG 9 cut(s) 147, 159, 326, 407, 680, 694, 700, 741, 1003
FaqI GGGAC 2 cut(s) 78, 1280
FatI CATG 9 cut(s) 143, 155, 322, 403, 676, 690, 696, 737, 999
FbaI TGATCA 2 cut(s) 187, 295
Fnu4HI GCNGC 5 cut(s) 200, 620, 673, 1024, 1032
FokI GGATG 6 cut(s) 533, 585, 884, 908, 915, 1055
Fsp4HI GCNGC 5 cut(s) 200, 620, 673, 1024, 1032
FspBI CTAG 2 cut(s) 462, 800
GlaI GCGC 1 cut(s) 990
GluI GCNGC 5 cut(s) 200, 620, 673, 1024, 1032
GsaI CCCAGC 3 cut(s) 80, 611, 815
GsuI CTGGAG 2 cut(s) 766, 1002
HaeIII GGCC 4 cut(s) 48, 314, 1076, 1292
HapII CCGG 2 cut(s) 623, 1293
HhaI GCGC 1 cut(s) 991
Hin1II CATG 9 cut(s) 147, 159, 326, 407, 680, 694, 700, 741, 1003
Hin6I GCGC 1 cut(s) 989
HinP1I GCGC 1 cut(s) 989
HinfI GANTC 4 cut(s) 101, 211, 506, 1087
HpaII CCGG 2 cut(s) 623, 1293
HphI GGTGA 5 cut(s) 197, 377, 401, 1082, 1127
Hpy166II GTNNAC 3 cut(s) 455, 586, 757
Hpy188I TCNGA 6 cut(s) 255, 262, 848, 859, 1092, 1337
Hpy188III TCNNGA 5 cut(s) 235, 237, 299, 667, 1261
Hpy8I GTNNAC 3 cut(s) 455, 586, 757
Hpy99I CGWCG 3 cut(s) 311, 335, 542
HpyAV CCTTC 4 cut(s) 154, 1106, 1115, 1303
HpyCH4III ACNGT 1 cut(s) 970
HpyCH4IV ACGT 1 cut(s) 18
HpyCH4V TGCA 7 cut(s) 4, 134, 155, 529, 599, 700, 1034
HpyF10VI GCNNNNNNNGC 4 cut(s) 87, 161, 170, 986
HpyF3I CTNAG 4 cut(s) 91, 589, 816, 1334
HpySE526I ACGT 1 cut(s) 18
Hsp92II CATG 9 cut(s) 147, 159, 326, 407, 680, 694, 700, 741, 1003
HspAI GCGC 1 cut(s) 989
Ksp22I TGATCA 2 cut(s) 187, 295
Kzo9I GATC 7 cut(s) 187, 295, 367, 638, 685, 722, 1195
LmnI GCTCC 4 cut(s) 32, 87, 996, 1092
Lsp1109I GCAGC 3 cut(s) 211, 684, 1018
LweI GCATC 5 cut(s) 160, 538, 886, 937, 976
MaeI CTAG 2 cut(s) 462, 800
MaeII ACGT 1 cut(s) 18
MaeIII GTNAC 1 cut(s) 267
MalI GATC 7 cut(s) 189, 297, 369, 640, 687, 724, 1197
MboI GATC 7 cut(s) 187, 295, 367, 638, 685, 722, 1195
MboII GAAGA 7 cut(s) 206, 474, 648, 815, 1096, 1130, 1318
MflI RGATCY 2 cut(s) 638, 722
MluCI AATT 4 cut(s) 39, 240, 408, 1101
MlyI GAGTC 1 cut(s) 205
MmeI TCCRAC 1 cut(s) 550
Mph1103I ATGCAT 1 cut(s) 157
MseI TTAA 4 cut(s) 273, 519, 836, 1345
MslI CAYNNNNRTG 1 cut(s) 695
MspCI CTTAAG 1 cut(s) 272
MspI CCGG 2 cut(s) 623, 1293
MspR9I CCNGG 3 cut(s) 62, 1289, 1294
MvaI CCWGG 2 cut(s) 62, 1289
MvnI CGCG 1 cut(s) 330
MwoI GCNNNNNNNGC 4 cut(s) 87, 161, 170, 986
NciI CCSGG 1 cut(s) 1294
NdeII GATC 7 cut(s) 187, 295, 367, 638, 685, 722, 1195
NlaIII CATG 9 cut(s) 147, 159, 326, 407, 680, 694, 700, 741, 1003
NlaIV GGNNCC 3 cut(s) 469, 544, 1094
NmuCI GTSAC 1 cut(s) 267
NsiI ATGCAT 1 cut(s) 157
NspI RCATGY 1 cut(s) 326
PaeR7I CTCGAG 1 cut(s) 235
PasI CCCWGGG 1 cut(s) 61
PceI AGGCCT 1 cut(s) 1076
PcsI WCGNNNNNNNCGW 1 cut(s) 315
PfeI GAWTC 3 cut(s) 101, 506, 1087
PflMI CCANNNNNTGG 1 cut(s) 1013
PkrI GCNGC 5 cut(s) 201, 621, 674, 1025, 1033
PleI GAGTC 1 cut(s) 205
PpsI GAGTC 1 cut(s) 205
Psp6I CCWGG 2 cut(s) 60, 1287
PspFI CCCAGC 3 cut(s) 76, 607, 811
PspGI CCWGG 2 cut(s) 60, 1287
PspN4I GGNNCC 3 cut(s) 469, 544, 1094
PsuI RGATCY 2 cut(s) 638, 722
RsaI GTAC 5 cut(s) 327, 704, 764, 967, 1207
RsaNI GTAC 5 cut(s) 326, 703, 763, 966, 1206
RseI CAYNNNNRTG 1 cut(s) 695
SaqAI TTAA 4 cut(s) 273, 519, 836, 1345
SatI GCNGC 5 cut(s) 200, 620, 673, 1024, 1032
Sau3AI GATC 7 cut(s) 187, 295, 367, 638, 685, 722, 1195
ScaI AGTACT 1 cut(s) 704
SchI GAGTC 1 cut(s) 205
ScrFI CCNGG 3 cut(s) 62, 1289, 1294
SfaNI GCATC 5 cut(s) 160, 538, 886, 937, 976
Sfr274I CTCGAG 1 cut(s) 235
SlaI CTCGAG 1 cut(s) 235
SmiMI CAYNNNNRTG 1 cut(s) 695
SmlI CTYRAG 4 cut(s) 115, 235, 272, 1261
SmoI CTYRAG 4 cut(s) 115, 235, 272, 1261
Sse9I AATT 4 cut(s) 39, 240, 408, 1101
SseBI AGGCCT 1 cut(s) 1076
SsiI CCGC 4 cut(s) 57, 620, 1023, 1300
SspI AATATT 2 cut(s) 341, 955
SspMI CTAG 2 cut(s) 462, 800
StuI AGGCCT 1 cut(s) 1076
StyD4I CCNGG 3 cut(s) 60, 1287, 1292
StyI CCWWGG 2 cut(s) 558, 932
TaaI ACNGT 1 cut(s) 970
TaiI ACGT 1 cut(s) 21
TaqI TCGA 2 cut(s) 236, 649
TasI AATT 4 cut(s) 39, 240, 408, 1101
TatI WGTACW 2 cut(s) 702, 1205
TauI GCSGC 2 cut(s) 622, 1026
TfiI GAWTC 3 cut(s) 101, 506, 1087
Tru1I TTAA 4 cut(s) 273, 519, 836, 1345
Tru9I TTAA 4 cut(s) 273, 519, 836, 1345
TscAI CASTG 3 cut(s) 597, 892, 1177
TseFI GTSAC 1 cut(s) 267
TseI GCWGC 3 cut(s) 199, 672, 1031
Tsp45I GTSAC 1 cut(s) 267
TspDTI ATGAA 5 cut(s) 343, 420, 685, 876, 1125
TspGWI ACGGA 3 cut(s) 348, 360, 555
TspRI CASTG 3 cut(s) 597, 892, 1177
Van91I CCANNNNNTGG 1 cut(s) 1013
Vha464I CTTAAG 1 cut(s) 272
XapI RAATTY 3 cut(s) 39, 240, 408
XceI RCATGY 1 cut(s) 326
XhoI CTCGAG 1 cut(s) 235
XspI CTAG 2 cut(s) 462, 800
ZrmI AGTACT 1 cut(s) 704
Zsp2I ATGCAT 1 cut(s) 157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.