FvH4_3g23690

metal ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000343
Physical Location & Seq
Reverse (-)
16776187 .. 16787618
11432 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g23690.t1

Sequence Viewer

Length: 3696 bp
ATGGAATCATCAGACGACGAGGCCGAGGCGGTGCCGGTCTCGGTTTCGAATTACCACTTCCTAGACGGGGAGGATGAGCCGATTTCCTTCCACGTGTTGCCAATTCAATGGAGCGATGGCGGTGAGAGGCAGGAAGAGAAGAAGAAAGCAGCAGTGTTGTTTCTGAAGGGCAGTGCTGACCTTGAGAAGATTTACAAGCCTGTGGTTGCTTGGAGGTTTGATCTCTCGAATGTGAAGCCCGAGATTGCGGTGCTTTCCAAGGAGGGGGATTGGATTGTGCTGCAGAAGCCGAGGAAGAGCTACGAGGGGATTATCAGGACTGTTTTGATCACTGTGCAGTGTCTTAGTTATGCGAAACGCAATCCCGAGGCGTCCGGGAAAGCTGTGTGGGATTACTTGTGTAAGGCTTTTAGCTATGAGGATAGGCCTTCAAAGAGTGATTTGGTGGATCAGAGGAGTCTGATTAGTGAAGCTCTTAAACGGGATGATGCGTTGGCGAAGTCCAAGTTTATGGTCGACTTTCTTAAAGAGAAGCCCACACTATCCGATGAGGATATTCAAGCTACGACAAAGCCAGGATTTATAGTTGATGATGCAGAAGATTATATGATTGATGTTGAAGATGAGTCAAATGATGATGATGATGATAACCTGTTTGATTCTGTTTGTGCCTTTTGTGATAACGGGGGACAGCTTTTGTGTTGTGAAGGGAGATGCCTAAGGTCCTTCCATCCCACTGTAGAGGATGGCGAGGATTCAATTTGTGAATCTCTTGGATTCACTCGGGAGGAAGTAAATGCAATGCCAAGTTTCTTCTGCAAGAACTGTCAATATAAGCAGCATCAGTGCTTTGCTTGTGGGAAGTTAGGCTCCTCAGATAAGTCTTTGGGCGCGGAGGTATTTCCATGTGTTTCTGCAACTTGTGGTCAATTTTACCATCCTCGGTGTGTTGCAAAACTAATCTATCAAGACAATGGAGTTTCTGCTGAAGAACTTGAGAAAAAGATCTCTCAGGGAGAATCTTTTACGTGTCCGATTCATAAGTGCTTTCTTTGTAAGCAAGGGGAGAATAAGAAGGATTCCGAAATGCGGTTTGCTGTGTGCAGGCGTTGTCCTAAATCTTATCACAGAAAATGCTTGCCGAGTAATATCCGTTTTGAGAAAACAGAGGAAGATAAGGAGGAAGAGATAGAAGATGAAGAAGAAACAGAAACAAGAGCTTGGGAAGGTCTATTGCCTAATCGCATACTAATATATTGCACAGAACATGAAATAGATGAAGAAATTGGAACTCCAATAAGGAACCATGTTAAATTCCCTGATGATGATGGAAAGAAGAATACTATTGTGAAAAAGAAGGCTACTTTCGAAGTTAAAAAGAGGAGACTGACATCAGAGTCTCATGTGGTCAGTGACAGCTCTCTTTTGAAGAAGAGAAAGCTTTCGTCAGAGGGATTGCATAGAGAAAGAACTGCTCCTACACTATCTAAGCAGAAAACGAATTCGGGTGAAAAACTTGGGGGTAACAGATTTACTGAAAAAGTACCTTCTGGGTTAAATGTATCAAGAAAGGTGATGGTAAATAGGACACTGAAAAAGGAAGTCCCGACCAGCGTGGAAAAGAACAACTCATTGGGTAATAGGCTGTTTAAATATGTTAAGGAACATGGGTCAGTAAAGTTTGGGAAGAAGGATGAGCCTGATGATGCTGAACTAAATTCTGAAAAGATAGCATATTTCGATCCTACAACAAAAACATTGAGTGCAGCAGCTTCCTTAGATCCTGCTAGAGAAAGGAGATTATATGCTTTAATGAAAGATGCTGCCTCGTCAATAACATTGGAAGAGGTTATAGAAAAGCACAAAGTTCCATCCACTCATAAGTCCTCAAACAGATATGCTGTAGAAAGGAACATTACACAGGGAAAGGTGGAAGGCTCAGTTGAGGCTATTCGAACTGCGTTAAAGAAACTACAGGAAGGTTGCAGTATTGAAGATGCAGAGGCTGTTTGTGCTCCGGAGATTCTTTCCCAGATTTATAAGTGGAAGAACAAGCTTAAAGTATATCTTGCACCTTTCTTGCATGGGATGCGGTATACGTCCTTTGGTCGCCATTTCACGAAGGTGGAAAAACTGGAACAGATTGCTGATATGCTCCATTGGTACGTACAGAGTGGAGACACGATAGTGGACTTCTGTTGTGGCTCTAATGATTTCAGCATCGCAATGAAGAAAAAGCTTGAGGAGATGGGGAAGAAGTGCTATTTTAAAAATTATGACATCATTCATCCCAAGAATGATTTTTGCTTTGAGAAGAGAGATTGGATGACTGTTCAAAAACATGAATTACCCGATCGTAATAAATTGATTATGGGGTTGAACCCTCCTTTTGGAGTTAAAGCAGCTTTGGCAAACCAGTTTATTTCTAAGGCTCTGGAATTCAATCCGAAGCTCCTTATTCTTATTGTTCCACCAGAAACAAAAAGGTTACCATATGATCTGATATGGGAGGATGAACGGTTTTTGTCAGGAAAGTCCTTTTATCTCCCTGGATCTGTTGATGAGAATGACAAGCAAATGGATCAGTGGAATGTGACAGCACCTCCACTCTATCTTTGGAGTCATCCTGATTGGTCTGAGGCACACAGGGCTATTGCTCGAAAGGCTAGTCACGGTCCTATGTTACTAGGGCCAGGGAAAGATGTTCATTCAGTTGAGAATAAGGATGAAAATTCAGTTGAGAATAAGGATGAAAATCTGATGGTGGAAAATGCTTATTTGACACCAACTGGTAATTCCAGTGATTTTGTTGGAGTTGCAGGCGAGGGACATGAAGAGAGATCCTCCAAGAGAAATGGTGACAGAGGTTTCCGTGCAAGTTCTGGTAACCATAAGAACCAGGTTAATGAGATTTCTGAGAGGCGGCAGTGCGGTGGCAGAAAAAAGAATGGGAGACGGCAGTGTGGTGGCAGAAAAAAGAATGGGAGTGGAGTGGTTGAGTTGTCGCCAGACAAAAAACGTGATGGCGACAATTTTTCTAGTGAGATTCAGAAGGAAAGTTCTCCATCTAATGAGCAAAAGCGAAAACCGAATCAGCATCCCAGCAATTCTAGTTCAAGTGTGCATTTTGAAACAGCTTATGACAGGACCATAGCCAGAATCCCTGATGACACTGGTAGAAATGTCATGAGCAGTGAGGAGATTTATCCAATTTTCACGCATAGATGTCCCAGTGGTGCCAGTCCTAGCTCTAATTATATGGATGCGGACTTGGAGGAACCTGAAATTAGATGCAGGAGAGAGAGGTTAGATTCCATTGAGCATAGATATTCCCGGGGGATGGATGAAATACATGCACGGTTTTATGGGCATCAAGATTCTGATTTGCACAGAAGTAATTATATAGCGGGTCCACGTCAAGTTGCATTTCCGTCCACGTATGGGCATGCTGAGCATGGTTCAGCCGTTTACCCATCTCACAGAATGAACACGTCAATCATGGAGAGGTATTTACATCCGTTGGATGGGACGAGTGCCTTAGGAACCCAACCAGCGCTTGGGTATGTGTTCAATAGCAACCCGTACAACGATCTCAGGGCACCCCAACATGCTGATCAACGGCCCCCCTATGGTTTTGCTCCTGGTCCAAATCCTTACTTCTCAAACCGGCATTCAGCAGGTTTTCTGCCTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006325 GO:0006355 GO:0006950 GO:0006952 GO:0006996 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0009605 GO:0009607 GO:0009620 GO:0009791 GO:0009889 GO:0009893 GO:0009909 GO:0009911 GO:0009987 GO:0010228 GO:0010468 GO:0010556 GO:0010604 GO:0010638 GO:0016043 GO:0019219 GO:0019222 GO:0022414 GO:0023052 GO:0030154 GO:0031056 GO:0031058 GO:0031060 GO:0031062 GO:0031323 GO:0031325 GO:0031326 GO:0031399 GO:0031401 GO:0031440 GO:0031935 GO:0031937 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033043 GO:0033044 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0043954 GO:0044030 GO:0044424 GO:0044464 GO:0048366 GO:0048367 GO:0048468 GO:0048518 GO:0048522 GO:0048580 GO:0048582 GO:0048608 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0048869 GO:0050684 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051052 GO:0051094 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051276 GO:0051570 GO:0051574 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060968 GO:0061085 GO:0061087 GO:0061458 GO:0065007 GO:0070827 GO:0070828 GO:0070829 GO:0071840 GO:0080090 GO:0090436 GO:0097159 GO:0098542 GO:0099402 GO:0140110 GO:1900109 GO:1900111 GO:1900363 GO:1901363 GO:1902275 GO:1903311 GO:1903506 GO:1905269 GO:2000024 GO:2000026 GO:2000112 GO:2000241 GO:2000243 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNMT1-RFD

Protein Analysis

1232

Amino Acids

139.45

Weight (kDa)

6.3

Isoelectric Point (pI)

44.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNMT1-RFD PF12047 15 - 133 4.3e-06 Cytosine specific DNA methyltransferase replication foci domain
PHD_NSD PF22908 279 - 347 3.4e-20 Histone-lysine N-methyltransferase NSD-like, PHD zinc finger
Mtase_EDM2 PF26055 722 - 886 1.3e-86 EDM2 methyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000343)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48090 AT5G48090 AT5G48090 AT5G55390 AT5G55390 AT5G55390
fragaria_vesca FvH4_3g23690 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160
malus_domestica MD03G1191000.v1.1 MD11G1207800.v1.1 MD11G1208000.v1.1
prunus_persica Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227600_v2.0.a1 Prupe.4G227700_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1
pyrus_communis pycom01g03400 pycom03g14420 pycom11g18100 pycom11g18140 pycom11g18150 pycom11g18160
rosa_chinensis RchiOBHm_Chr2g0145981 RchiOBHm_Chr4g0405241 RchiOBHm_Chr4g0405271 RchiOBHm_Chr5g0016201 RchiOBHm_Chr5g0041831 RchiOBHm_Chr5g0041871 RchiOBHm_Chr5g0041881 RchiOBHm_Chr5g0059241
rosa_laevigata RLG00000002117 RLG00000008861 RLG00000008862 RLG00000011000 RLG00000033216 RLG00000034097 RLG00000034101
rosa_multiflora Rmu_sc0000161.1_g000001 Rmu_sc0000861.1_g000040 Rmu_sc0007522.1_g000003 Rmu_sc0009443.1_g000015 Rmu_sc0022129.1_g000001 Rmu_sc0042315.1_g000001
rosa_roxburghii Rroxscaffold_1G00038660 Rroxscaffold_1G00038690 Rroxscaffold_5G00349560 Rroxscaffold_5G00349610 Rroxscaffold_6G00388370 Rroxscaffold_7G00188740
rosa_rugosa Rorug03G0199700 Rorug04G0055400 Rorug04G0055500 Rorug04G0055900 Rorug04G0056000 Rorug04G0056100 Rorug04G0056100 Rorug04G0056200 Rorug05G0195600 Rorug05G0195700 Rorug05G0195800 Rorug05G0195900 Rorug05G0196800 Rorug05G0385000
rosa_samantha Rh4AG129900 Rh4AG130100 Rh4BG124200 Rh4BG124400 Rh4BG124500 Rh4CG137200 Rh4CG137300 Rh4DG123500 Rh4DG123700 Rh5AG280800 Rh5AG281400 Rh5BG286700 Rh5BG287000 Rh5BG397800 Rh5CG318100 Rh5CG318600 Rh5CG421600 Rh5DG132300 Rh5DG132400 Rh5DG295400 Rh5DG295700 Rh7BG475300
rosa_wichuraiana Rw0G001330 Rw0G005730 Rw4G008670 Rw4G010510 Rw4G010540 Rw5G026450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2040
AasI GACNNNNNNGTC 1 cut(s) 1396
Acc36I ACCTGC 1 cut(s) 3668
AccB1I GGYRCC 3 cut(s) 31, 3236, 3598
AccB7I CCANNNNNTGG 1 cut(s) 3557
AccI GTMKAC 2 cut(s) 516, 2096
AccII CGCG 1 cut(s) 893
AccIII TCCGGA 1 cut(s) 2017
AclWI GGATC 6 cut(s) 456, 1736, 1775, 2560, 2589, 2835
AcsI RAATTY 5 cut(s) 1313, 1501, 1717, 2438, 2731
AcuI CTGAAG 2 cut(s) 185, 1008
AcvI CACGTG 1 cut(s) 94
AcyI GRCGYC 1 cut(s) 371
AfaI GTAC 4 cut(s) 1545, 2165, 2169, 3584
AfeI AGCGCT 1 cut(s) 3555
AfiI CCNNNNNNNGG 9 cut(s) 67, 264, 1089, 2390, 3340, 3441, 3524, 3557, 3629
AflIII ACRYGT 3 cut(s) 93, 1028, 3489
AjiI CACGTC 2 cut(s) 3416, 3492
AjnI CCWGG 5 cut(s) 574, 2548, 2692, 2898, 3640
AjuI GAANNNNNNNTTGG 4 cut(s) 1616, 1648, 2779, 2811
AleI CACNNNNGTG 2 cut(s) 2123, 2186
Alw21I GWGCWC 1 cut(s) 2017
Alw26I GTCTC 5 cut(s) 43, 1378, 1404, 2172, 2947
AlwI GGATC 6 cut(s) 456, 1736, 1775, 2560, 2589, 2835
AlwNI CAGNNNCTG 1 cut(s) 2006
Ama87I CYCGRG 4 cut(s) 239, 365, 783, 3333
Aor13HI TCCGGA 1 cut(s) 2017
Aor51HI AGCGCT 1 cut(s) 3555
AoxI GGCC 4 cut(s) 21, 425, 2690, 3620
ApeKI GCWGC 7 cut(s) 149, 280, 838, 1766, 1769, 1823, 2402
ApoI RAATTY 5 cut(s) 1313, 1501, 1717, 2438, 2731
ArsI GACNNNNNNTTYG 2 cut(s) 2653, 2685
Asp700I GAANNNNTTC 1 cut(s) 1441
AspLEI GCGC 2 cut(s) 893, 3556
AspS9I GGNCC 7 cut(s) 723, 2675, 2690, 3147, 3410, 3621, 3644
AsuC2I CCSGG 3 cut(s) 376, 3334, 3335
AsuHPI GGTGA 4 cut(s) 134, 1520, 1585, 2870
AsuII TTCGAA 3 cut(s) 47, 1368, 1954
AvaI CYCGRG 4 cut(s) 239, 365, 783, 3333
AvaII GGWCC 5 cut(s) 723, 2675, 3147, 3410, 3644
AxyI CCTNAGG 2 cut(s) 719, 3538
BaeGI GKGCMC 1 cut(s) 3601
BanI GGYRCC 3 cut(s) 31, 3236, 3598
BbrPI CACGTG 1 cut(s) 94
Bbv12I GWGCWC 1 cut(s) 2017
BbvI GCAGC 7 cut(s) 161, 267, 850, 1778, 1781, 1810, 2414
BceAI ACGGC 3 cut(s) 2972, 3449, 3635
BciT130I CCWGG 5 cut(s) 576, 2550, 2694, 2900, 3642
BclI TGATCA 2 cut(s) 327, 3613
BcnI CCSGG 3 cut(s) 376, 3334, 3335
BcoDI GTCTC 5 cut(s) 43, 1378, 1404, 2172, 2947
BfaI CTAG 7 cut(s) 62, 1788, 2667, 2687, 3039, 3111, 3246
BfmI CTRYAG 4 cut(s) 281, 738, 1902, 1973
BfoI RGCGCY 1 cut(s) 3557
BfuAI ACCTGC 1 cut(s) 3668
BglII AGATCT 1 cut(s) 1005
BisI GCNGC 8 cut(s) 150, 281, 839, 1767, 1770, 1824, 2403, 2924
BlpI GCTNAGC 1 cut(s) 3450
BlsI GCNGC 8 cut(s) 151, 282, 840, 1768, 1771, 1825, 2404, 2925
Bme1390I CCNGG 8 cut(s) 376, 576, 2550, 2694, 2900, 3334, 3335, 3642
Bme18I GGWCC 5 cut(s) 723, 2675, 3147, 3410, 3644
BmeT110I CYCGRG 4 cut(s) 239, 365, 783, 3333
BmgBI CACGTC 2 cut(s) 3416, 3492
BmgT120I GGNCC 7 cut(s) 723, 2675, 2690, 3147, 3410, 3621, 3644
BmiI GGNNCC 9 cut(s) 33, 871, 1304, 3238, 3279, 3411, 3544, 3600, 3623
BmrFI CCNGG 8 cut(s) 376, 576, 2550, 2694, 2900, 3334, 3335, 3642
BmrI ACTGGG 1 cut(s) 3225
BmuI ACTGGG 1 cut(s) 3225
BplI GAGNNNNNCTC 2 cut(s) 2828, 2860
Bpu1102I GCTNAGC 1 cut(s) 3450
Bpu14I TTCGAA 3 cut(s) 47, 1368, 1954
BpuEI CTTGAG 3 cut(s) 203, 1016, 2261
BpuMI CCSGG 3 cut(s) 376, 3334, 3335
BsaAI YACGTR 4 cut(s) 94, 1029, 2167, 3438
BsaBI GATNNNNATC 1 cut(s) 2754
BsaHI GRCGYC 1 cut(s) 371
BsaI GGTCTC 1 cut(s) 43
BsaJI CCNNGG 9 cut(s) 24, 258, 290, 366, 941, 2548, 2693, 3333, 3334
BsaWI WCCGGW 1 cut(s) 2017
BsaXI ACNNNNNCTCC 2 cut(s) 2587, 2617
Bsc4I CCNNNNNNNGG 9 cut(s) 67, 264, 1089, 2390, 3340, 3441, 3524, 3557, 3629
Bse118I RCCGGY 2 cut(s) 34, 3666
Bse1I ACTGG 7 cut(s) 2139, 2416, 2794, 2799, 3178, 3231, 3240
Bse21I CCTNAGG 2 cut(s) 719, 3538
Bse3DI GCAATG 2 cut(s) 807, 2232
Bse8I GATNNNNATC 1 cut(s) 2754
BseAI TCCGGA 1 cut(s) 2017
BseBI CCWGG 5 cut(s) 576, 2550, 2694, 2900, 3642
BseDI CCNNGG 9 cut(s) 24, 258, 290, 366, 941, 2548, 2693, 3333, 3334
BseJI GATNNNNATC 1 cut(s) 2754
BseLI CCNNNNNNNGG 9 cut(s) 67, 264, 1089, 2390, 3340, 3441, 3524, 3557, 3629
BseMI GCAATG 2 cut(s) 807, 2232
BseMII CTCAG 8 cut(s) 888, 1025, 1953, 2628, 2907, 3441, 3607, 3681
BseNI ACTGG 7 cut(s) 2139, 2416, 2794, 2799, 3178, 3231, 3240
BseRI GAGGAG 5 cut(s) 469, 862, 1396, 2258, 3212
BseSI GKGCMC 1 cut(s) 3601
BseXI GCAGC 7 cut(s) 161, 267, 850, 1778, 1781, 1810, 2414
BseYI CCCAGC 1 cut(s) 3101
BsgI GTGCAG 3 cut(s) 356, 1123, 1785
Bsh1236I CGCG 1 cut(s) 893
Bsh1285I CGRYCG 1 cut(s) 2356
BshFI GGCC 4 cut(s) 23, 427, 2692, 3622
BshNI GGYRCC 3 cut(s) 31, 3236, 3598
BsiEI CGRYCG 1 cut(s) 2356
BsiHKAI GWGCWC 1 cut(s) 2017
BsiHKCI CYCGRG 4 cut(s) 239, 365, 783, 3333
BsiSI CCGG 5 cut(s) 35, 375, 2018, 3334, 3667
BslFI GGGAC 5 cut(s) 702, 1589, 2841, 3213, 3541
BslI CCNNNNNNNGG 9 cut(s) 67, 264, 1089, 2390, 3340, 3441, 3524, 3557, 3629
BsmAI GTCTC 5 cut(s) 43, 1378, 1404, 2172, 2947
BsmBI CGTCTC 1 cut(s) 2947
BsmFI GGGAC 5 cut(s) 702, 1589, 2841, 3213, 3541
BsmI GAATGC 1 cut(s) 3670
BsnI GGCC 4 cut(s) 23, 427, 2692, 3622
Bso31I GGTCTC 1 cut(s) 43
BsoBI CYCGRG 4 cut(s) 239, 365, 783, 3333
Bsp119I TTCGAA 3 cut(s) 47, 1368, 1954
Bsp1286I GDGCHC 2 cut(s) 2017, 3601
Bsp13I TCCGGA 1 cut(s) 2017
Bsp1720I GCTNAGC 1 cut(s) 3450
BspANI GGCC 4 cut(s) 23, 427, 2692, 3622
BspCNI CTCAG 8 cut(s) 887, 1024, 1952, 2629, 2908, 3442, 3606, 3682
BspEI TCCGGA 1 cut(s) 2017
BspFNI CGCG 1 cut(s) 893
BspHI TCATGA 1 cut(s) 3186
BspLI GGNNCC 9 cut(s) 33, 871, 1304, 3238, 3279, 3411, 3544, 3600, 3623
BspMAI CTGCAG 1 cut(s) 285
BspMI ACCTGC 1 cut(s) 3668
BspPI GGATC 6 cut(s) 456, 1736, 1775, 2560, 2589, 2835
BspQI GCTCTTC 1 cut(s) 290
BspT104I TTCGAA 3 cut(s) 47, 1368, 1954
BspT107I GGYRCC 3 cut(s) 31, 3236, 3598
BspTNI GGTCTC 1 cut(s) 43
BsrDI GCAATG 2 cut(s) 807, 2232
BsrFI RCCGGY 2 cut(s) 34, 3666
BsrI ACTGG 7 cut(s) 2139, 2416, 2794, 2799, 3178, 3231, 3240
BssAI RCCGGY 2 cut(s) 34, 3666
BssECI CCNNGG 9 cut(s) 24, 258, 290, 366, 941, 2548, 2693, 3333, 3334
BssNAI GTATAC 1 cut(s) 2097
BssNI GRCGYC 1 cut(s) 371
BssT1I CCWWGG 1 cut(s) 258
Bst1107I GTATAC 1 cut(s) 2097
Bst2UI CCWGG 5 cut(s) 576, 2550, 2694, 2900, 3642
Bst4CI ACNGT 8 cut(s) 322, 334, 739, 827, 2332, 2520, 2675, 3360
Bst6I CTCTTC 7 cut(s) 129, 290, 1179, 1427, 1839, 2309, 2829
BstACI GRCGYC 1 cut(s) 371
BstAPI GCANNNNNTGC 1 cut(s) 2089
BstBAI YACGTR 4 cut(s) 94, 1029, 2167, 3438
BstBI TTCGAA 3 cut(s) 47, 1368, 1954
BstC8I GCNNGC 4 cut(s) 1106, 1139, 2821, 3447
BstEII GGTNACC 2 cut(s) 2487, 2885
BstFNI CGCG 1 cut(s) 893
BstH2I RGCGCY 1 cut(s) 3557
BstHHI GCGC 2 cut(s) 893, 3556
BstMAI GTCTC 5 cut(s) 43, 1378, 1404, 2172, 2947
BstMCI CGRYCG 1 cut(s) 2356
BstMWI GCNNNNNNNGC 7 cut(s) 286, 2012, 2089, 2408, 2648, 2663, 3451
BstNI CCWGG 5 cut(s) 576, 2550, 2694, 2900, 3642
BstNSI RCATGY 3 cut(s) 3356, 3449, 3611
BstPI GGTNACC 2 cut(s) 2487, 2885
BstSCI CCNGG 8 cut(s) 374, 574, 2548, 2692, 2898, 3332, 3333, 3640
BstSFI CTRYAG 4 cut(s) 281, 738, 1902, 1973
BstSLI GKGCMC 1 cut(s) 3601
BstSNI TACGTA 1 cut(s) 2167
BstUI CGCG 1 cut(s) 893
BstV1I GCAGC 7 cut(s) 161, 267, 850, 1778, 1781, 1810, 2414
BstX2I RGATCY 4 cut(s) 1005, 1780, 2552, 2840
BstXI CCANNNNNNTGG 2 cut(s) 108, 511
BstYI RGATCY 4 cut(s) 1005, 1780, 2552, 2840
BstZ17I GTATAC 1 cut(s) 2097
Bsu36I CCTNAGG 2 cut(s) 719, 3538
BsuRI GGCC 4 cut(s) 23, 427, 2692, 3622
BtgZI GCGATG 2 cut(s) 129, 2206
BtrI CACGTC 2 cut(s) 3416, 3492
BtsI GCAGTG 6 cut(s) 159, 178, 344, 2933, 2966, 3199
BveI ACCTGC 1 cut(s) 3668
Cac8I GCNNGC 4 cut(s) 1106, 1139, 2821, 3447
CaiI CAGNNNCTG 1 cut(s) 2006
CciI TCATGA 1 cut(s) 3186
CfoI GCGC 2 cut(s) 893, 3556
Cfr10I RCCGGY 2 cut(s) 34, 3666
Cfr13I GGNCC 7 cut(s) 723, 2675, 2690, 3147, 3410, 3621, 3644
Cfr9I CCCGGG 1 cut(s) 3333
CseI GACGC 1 cut(s) 360
CsiI ACCWGGT 1 cut(s) 2898
Csp6I GTAC 4 cut(s) 1544, 2164, 2168, 3583
CviQI GTAC 4 cut(s) 1544, 2164, 2168, 3583
DraI TTTAAA 2 cut(s) 1651, 2269
DrdI GACNNNNNNGTC 1 cut(s) 1396
DseDI GACNNNNNNGTC 1 cut(s) 1396
Eam1104I CTCTTC 7 cut(s) 129, 290, 1179, 1427, 1839, 2309, 2829
EarI CTCTTC 7 cut(s) 129, 290, 1179, 1427, 1839, 2309, 2829
Eco105I TACGTA 1 cut(s) 2167
Eco130I CCWWGG 1 cut(s) 258
Eco147I AGGCCT 1 cut(s) 427
Eco31I GGTCTC 1 cut(s) 43
Eco47I GGWCC 5 cut(s) 723, 2675, 3147, 3410, 3644
Eco47III AGCGCT 1 cut(s) 3555
Eco57I CTGAAG 2 cut(s) 185, 1008
Eco72I CACGTG 1 cut(s) 94
Eco81I CCTNAGG 2 cut(s) 719, 3538
Eco88I CYCGRG 4 cut(s) 239, 365, 783, 3333
Eco91I GGTNACC 2 cut(s) 2487, 2885
EcoO109I RGGNCCY 1 cut(s) 723
EcoO65I GGTNACC 2 cut(s) 2487, 2885
EcoRI GAATTC 2 cut(s) 1501, 2438
EcoRII CCWGG 5 cut(s) 574, 2548, 2692, 2898, 3640
EcoT14I CCWWGG 1 cut(s) 258
ErhI CCWWGG 1 cut(s) 258
Esp3I CGTCTC 1 cut(s) 2947
FalI AAGNNNNNCTT 2 cut(s) 2052, 2084
FaqI GGGAC 5 cut(s) 702, 1589, 2841, 3213, 3541
FauI CCCGC 1 cut(s) 3400
FauNDI CATATG 1 cut(s) 2494
FbaI TGATCA 2 cut(s) 327, 3613
FblI GTMKAC 2 cut(s) 516, 2096
Fnu4HI GCNGC 8 cut(s) 150, 281, 839, 1767, 1770, 1824, 2403, 2924
Fsp4HI GCNGC 8 cut(s) 150, 281, 839, 1767, 1770, 1824, 2403, 2924
FspBI CTAG 7 cut(s) 62, 1788, 2667, 2687, 3039, 3111, 3246
GlaI GCGC 2 cut(s) 892, 3555
GluI GCNGC 8 cut(s) 150, 281, 839, 1767, 1770, 1824, 2403, 2924
GsaI CCCAGC 1 cut(s) 3105
HaeII RGCGCY 1 cut(s) 3557
HaeIII GGCC 4 cut(s) 23, 427, 2692, 3622
HapII CCGG 5 cut(s) 35, 375, 2018, 3334, 3667
HgaI GACGC 1 cut(s) 360
HhaI GCGC 2 cut(s) 893, 3556
Hin1I GRCGYC 1 cut(s) 371
Hin6I GCGC 2 cut(s) 891, 3554
HinP1I GCGC 2 cut(s) 891, 3554
HincII GTYRAC 1 cut(s) 517
HindII GTYRAC 1 cut(s) 517
HindIII AAGCTT 3 cut(s) 1439, 2054, 2237
HpaII CCGG 5 cut(s) 35, 375, 2018, 3334, 3667
HphI GGTGA 4 cut(s) 134, 1520, 1585, 2870
Hpy166II GTNNAC 6 cut(s) 517, 2097, 2191, 3413, 3435, 3469
Hpy8I GTNNAC 6 cut(s) 517, 2097, 2191, 3413, 3435, 3469
Hpy99I CGWCG 1 cut(s) 20
HpyCH4III ACNGT 8 cut(s) 322, 334, 739, 827, 2332, 2520, 2675, 3360
HpyCH4IV ACGT 8 cut(s) 93, 1028, 2099, 2166, 3019, 3415, 3437, 3491
HpyF10VI GCNNNNNNNGC 7 cut(s) 286, 2012, 2089, 2408, 2648, 2663, 3451
HpySE526I ACGT 8 cut(s) 93, 1028, 2099, 2166, 3019, 3415, 3437, 3491
Hsp92I GRCGYC 1 cut(s) 371
HspAI GCGC 2 cut(s) 891, 3554
Kpn2I TCCGGA 1 cut(s) 2017
Ksp22I TGATCA 2 cut(s) 327, 3613
LguI GCTCTTC 1 cut(s) 290
LmnI GCTCC 7 cut(s) 111, 875, 1480, 2020, 2160, 2457, 3643
Lsp1109I GCAGC 7 cut(s) 161, 267, 850, 1778, 1781, 1810, 2414
MabI ACCWGGT 1 cut(s) 2898
MaeI CTAG 7 cut(s) 62, 1788, 2667, 2687, 3039, 3111, 3246
MaeII ACGT 8 cut(s) 93, 1028, 2099, 2166, 3019, 3415, 3437, 3491
MaeIII GTNAC 8 cut(s) 1412, 1523, 2487, 2593, 2669, 2682, 2858, 2885
MflI RGATCY 4 cut(s) 1005, 1780, 2552, 2840
MhlI GDGCHC 2 cut(s) 2017, 3601
MlyI GAGTC 4 cut(s) 466, 635, 1406, 2629
MmeI TCCRAC 2 cut(s) 2791, 3501
MroI TCCGGA 1 cut(s) 2017
MroXI GAANNNNTTC 1 cut(s) 1441
MslI CAYNNNNRTG 3 cut(s) 2123, 2186, 2225
MspI CCGG 5 cut(s) 35, 375, 2018, 3334, 3667
MspR9I CCNGG 8 cut(s) 376, 576, 2550, 2694, 2900, 3334, 3335, 3642
Mva1269I GAATGC 1 cut(s) 3670
MvaI CCWGG 5 cut(s) 576, 2550, 2694, 2900, 3642
MvnI CGCG 1 cut(s) 893
MwoI GCNNNNNNNGC 7 cut(s) 286, 2012, 2089, 2408, 2648, 2663, 3451
NciI CCSGG 3 cut(s) 376, 3334, 3335
NdeI CATATG 1 cut(s) 2494
NlaIV GGNNCC 9 cut(s) 33, 871, 1304, 3238, 3279, 3411, 3544, 3600, 3623
NmeAIII GCCGAG 3 cut(s) 49, 315, 1167
NmuCI GTSAC 4 cut(s) 1412, 2593, 2669, 2858
NspI RCATGY 3 cut(s) 3356, 3449, 3611
NspV TTCGAA 3 cut(s) 47, 1368, 1954
OliI CACNNNNGTG 2 cut(s) 2123, 2186
PaeI GCATGC 1 cut(s) 3449
PagI TCATGA 1 cut(s) 3186
PceI AGGCCT 1 cut(s) 427
PciSI GCTCTTC 1 cut(s) 290
PcsI WCGNNNNNNNCGW 1 cut(s) 21
PctI GAATGC 1 cut(s) 3670
PdmI GAANNNNTTC 1 cut(s) 1441
PflMI CCANNNNNTGG 1 cut(s) 3557
PfoI TCCNGGA 1 cut(s) 374
PkrI GCNGC 8 cut(s) 151, 282, 840, 1768, 1771, 1825, 2404, 2925
Ple19I CGATCG 1 cut(s) 2356
PleI GAGTC 4 cut(s) 465, 634, 1405, 2628
PmaCI CACGTG 1 cut(s) 94
PmlI CACGTG 1 cut(s) 94
PpsI GAGTC 4 cut(s) 465, 634, 1405, 2628
Ppu21I YACGTR 4 cut(s) 94, 1029, 2167, 3438
PpuMI RGGWCCY 1 cut(s) 723
PsiI TTATAA 1 cut(s) 2040
Psp5II RGGWCCY 1 cut(s) 723
Psp6I CCWGG 5 cut(s) 574, 2548, 2692, 2898, 3640
PspCI CACGTG 1 cut(s) 94
PspEI GGTNACC 2 cut(s) 2487, 2885
PspFI CCCAGC 1 cut(s) 3101
PspGI CCWGG 5 cut(s) 574, 2548, 2692, 2898, 3640
PspN4I GGNNCC 9 cut(s) 33, 871, 1304, 3238, 3279, 3411, 3544, 3600, 3623
PspPI GGNCC 7 cut(s) 723, 2675, 2690, 3147, 3410, 3621, 3644
PspPPI RGGWCCY 1 cut(s) 723
PsrI GAACNNNNNNTAC 2 cut(s) 1462, 1494
PstI CTGCAG 1 cut(s) 285
PstNI CAGNNNCTG 1 cut(s) 2006
PsuI RGATCY 4 cut(s) 1005, 1780, 2552, 2840
PvuI CGATCG 1 cut(s) 2356
RsaI GTAC 4 cut(s) 1545, 2165, 2169, 3584
RsaNI GTAC 4 cut(s) 1544, 2164, 2168, 3583
RseI CAYNNNNRTG 3 cut(s) 2123, 2186, 2225
SalI GTCGAC 1 cut(s) 515
SapI GCTCTTC 1 cut(s) 290
SatI GCNGC 8 cut(s) 150, 281, 839, 1767, 1770, 1824, 2403, 2924
Sau96I GGNCC 7 cut(s) 723, 2675, 2690, 3147, 3410, 3621, 3644
SchI GAGTC 4 cut(s) 466, 635, 1406, 2629
ScrFI CCNGG 8 cut(s) 376, 576, 2550, 2694, 2900, 3334, 3335, 3642
SduI GDGCHC 2 cut(s) 2017, 3601
SexAI ACCWGGT 1 cut(s) 2898
SfcI CTRYAG 4 cut(s) 281, 738, 1902, 1973
SfuI TTCGAA 3 cut(s) 47, 1368, 1954
SinI GGWCC 5 cut(s) 723, 2675, 3147, 3410, 3644
SmaI CCCGGG 1 cut(s) 3335
SmiMI CAYNNNNRTG 3 cut(s) 2123, 2186, 2225
SmlI CTYRAG 3 cut(s) 182, 995, 2240
SmoI CTYRAG 3 cut(s) 182, 995, 2240
SnaBI TACGTA 1 cut(s) 2167
SphI GCATGC 1 cut(s) 3449
SseBI AGGCCT 1 cut(s) 427
SspMI CTAG 7 cut(s) 62, 1788, 2667, 2687, 3039, 3111, 3246
StuI AGGCCT 1 cut(s) 427
StyD4I CCNGG 8 cut(s) 374, 574, 2548, 2692, 2898, 3332, 3333, 3640
StyI CCWWGG 1 cut(s) 258
TaaI ACNGT 8 cut(s) 322, 334, 739, 827, 2332, 2520, 2675, 3360
TaiI ACGT 8 cut(s) 96, 1031, 2102, 2169, 3022, 3418, 3440, 3494
TaqI TCGA 7 cut(s) 47, 227, 516, 1368, 1740, 1954, 2659
TauI GCSGC 1 cut(s) 2926
TseFI GTSAC 4 cut(s) 1412, 2593, 2669, 2858
TseI GCWGC 7 cut(s) 149, 280, 838, 1766, 1769, 1823, 2402
Tsp45I GTSAC 4 cut(s) 1412, 2593, 2669, 2858
TspGWI ACGGA 4 cut(s) 1142, 2861, 3420, 3507
TspMI CCCGGG 1 cut(s) 3333
Van91I CCANNNNNTGG 1 cut(s) 3557
VpaK11BI GGWCC 5 cut(s) 723, 2675, 3147, 3410, 3644
XapI RAATTY 5 cut(s) 1313, 1501, 1717, 2438, 2731
XceI RCATGY 3 cut(s) 3356, 3449, 3611
XcmI CCANNNNNNNNNTGG 2 cut(s) 2613, 3554
XmaI CCCGGG 1 cut(s) 3333
XmiI GTMKAC 2 cut(s) 516, 2096
XmnI GAANNNNTTC 1 cut(s) 1441
XspI CTAG 7 cut(s) 62, 1788, 2667, 2687, 3039, 3111, 3246
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.