metal ion binding
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 314 |
| AcoI | YGGCCR | 2 cut(s) | 96, 339 |
| AcsI | RAATTY | 1 cut(s) | 203 |
| AgsI | TTSAA | 2 cut(s) | 22, 58 |
| AluBI | AGCT | 2 cut(s) | 17, 179 |
| AluI | AGCT | 2 cut(s) | 17, 179 |
| Alw26I | GTCTC | 1 cut(s) | 184 |
| AoxI | GGCC | 2 cut(s) | 96, 339 |
| ApeKI | GCWGC | 2 cut(s) | 29, 155 |
| ApoI | RAATTY | 1 cut(s) | 203 |
| BaeGI | GKGCMC | 1 cut(s) | 317 |
| BalI | TGGCCA | 1 cut(s) | 341 |
| BanI | GGYRCC | 1 cut(s) | 314 |
| BbvI | GCAGC | 2 cut(s) | 16, 167 |
| BccI | CCATC | 3 cut(s) | 167, 302, 331 |
| BcoDI | GTCTC | 1 cut(s) | 184 |
| BfaI | CTAG | 1 cut(s) | 78 |
| BisI | GCNGC | 2 cut(s) | 30, 156 |
| BlsI | GCNGC | 2 cut(s) | 31, 157 |
| BmiI | GGNNCC | 1 cut(s) | 316 |
| BmsI | GCATC | 3 cut(s) | 121, 143, 151 |
| BsaJI | CCNNGG | 2 cut(s) | 240, 255 |
| BseDI | CCNNGG | 2 cut(s) | 240, 255 |
| BseGI | GGATG | 1 cut(s) | 342 |
| BseMII | CTCAG | 1 cut(s) | 345 |
| BseSI | GKGCMC | 1 cut(s) | 317 |
| BseXI | GCAGC | 2 cut(s) | 16, 167 |
| BshFI | GGCC | 2 cut(s) | 98, 341 |
| BshNI | GGYRCC | 1 cut(s) | 314 |
| BslFI | GGGAC | 1 cut(s) | 227 |
| BsmAI | GTCTC | 1 cut(s) | 184 |
| BsmFI | GGGAC | 1 cut(s) | 227 |
| BsnI | GGCC | 2 cut(s) | 98, 341 |
| Bsp1286I | GDGCHC | 1 cut(s) | 317 |
| Bsp143I | GATC | 1 cut(s) | 70 |
| Bsp19I | CCATGG | 1 cut(s) | 240 |
| BspANI | GGCC | 2 cut(s) | 98, 341 |
| BspCNI | CTCAG | 1 cut(s) | 346 |
| BspHI | TCATGA | 1 cut(s) | 207 |
| BspLI | GGNNCC | 1 cut(s) | 316 |
| BspT107I | GGYRCC | 1 cut(s) | 314 |
| BssECI | CCNNGG | 2 cut(s) | 240, 255 |
| BssMI | GATC | 1 cut(s) | 70 |
| BssT1I | CCWWGG | 2 cut(s) | 240, 255 |
| Bst4CI | ACNGT | 2 cut(s) | 220, 319 |
| Bst6I | CTCTTC | 1 cut(s) | 54 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 161 |
| BstDEI | CTNAG | 1 cut(s) | 354 |
| BstDSI | CCRYGG | 1 cut(s) | 240 |
| BstF5I | GGATG | 1 cut(s) | 342 |
| BstKTI | GATC | 1 cut(s) | 73 |
| BstMAI | GTCTC | 1 cut(s) | 184 |
| BstMBI | GATC | 1 cut(s) | 70 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 161 |
| BstSLI | GKGCMC | 1 cut(s) | 317 |
| BstV1I | GCAGC | 2 cut(s) | 16, 167 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 241 |
| BsuRI | GGCC | 2 cut(s) | 98, 341 |
| BtgI | CCRYGG | 1 cut(s) | 240 |
| BtsCI | GGATG | 1 cut(s) | 342 |
| BtsI | GCAGTG | 1 cut(s) | 228 |
| BtsIMutI | CAGTG | 1 cut(s) | 228 |
| CciI | TCATGA | 1 cut(s) | 207 |
| CviAII | CATG | 3 cut(s) | 208, 241, 330 |
| CviJI | RGCY | 4 cut(s) | 17, 98, 179, 341 |
| CviKI_1 | RGCY | 4 cut(s) | 17, 98, 179, 341 |
| DdeI | CTNAG | 1 cut(s) | 354 |
| DpnI | GATC | 1 cut(s) | 72 |
| DpnII | GATC | 1 cut(s) | 70 |
| EaeI | YGGCCR | 2 cut(s) | 96, 339 |
| Eam1104I | CTCTTC | 1 cut(s) | 54 |
| EarI | CTCTTC | 1 cut(s) | 54 |
| Eco130I | CCWWGG | 2 cut(s) | 240, 255 |
| EcoRI | GAATTC | 1 cut(s) | 203 |
| EcoT14I | CCWWGG | 2 cut(s) | 240, 255 |
| EcoT22I | ATGCAT | 1 cut(s) | 136 |
| ErhI | CCWWGG | 2 cut(s) | 240, 255 |
| FaeI | CATG | 3 cut(s) | 211, 244, 333 |
| FaiI | YATR | 4 cut(s) | 75, 209, 242, 331 |
| FaqI | GGGAC | 1 cut(s) | 227 |
| FatI | CATG | 3 cut(s) | 207, 240, 329 |
| Fnu4HI | GCNGC | 2 cut(s) | 30, 156 |
| FokI | GGATG | 1 cut(s) | 349 |
| Fsp4HI | GCNGC | 2 cut(s) | 30, 156 |
| FspBI | CTAG | 1 cut(s) | 78 |
| GluI | GCNGC | 2 cut(s) | 30, 156 |
| HaeIII | GGCC | 2 cut(s) | 98, 341 |
| Hin1II | CATG | 3 cut(s) | 211, 244, 333 |
| Hpy188I | TCNGA | 2 cut(s) | 70, 139 |
| Hpy188III | TCNNGA | 1 cut(s) | 208 |
| Hpy99I | CGWCG | 1 cut(s) | 268 |
| HpyAV | CCTTC | 1 cut(s) | 115 |
| HpyCH4III | ACNGT | 2 cut(s) | 220, 319 |
| HpyCH4V | TGCA | 6 cut(s) | 4, 32, 112, 134, 164, 329 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 161 |
| HpyF3I | CTNAG | 1 cut(s) | 354 |
| Hsp92II | CATG | 3 cut(s) | 211, 244, 333 |
| Kzo9I | GATC | 1 cut(s) | 70 |
| LmnI | GCTCC | 1 cut(s) | 176 |
| LpnPI | CCDG | 1 cut(s) | 309 |
| Lsp1109I | GCAGC | 2 cut(s) | 16, 167 |
| LweI | GCATC | 3 cut(s) | 121, 143, 151 |
| MaeI | CTAG | 1 cut(s) | 78 |
| MaeIII | GTNAC | 2 cut(s) | 88, 274 |
| MalI | GATC | 1 cut(s) | 72 |
| MboI | GATC | 1 cut(s) | 70 |
| MboII | GAAGA | 2 cut(s) | 41, 130 |
| MhlI | GDGCHC | 1 cut(s) | 317 |
| MlsI | TGGCCA | 1 cut(s) | 341 |
| MluCI | AATT | 1 cut(s) | 203 |
| MluNI | TGGCCA | 1 cut(s) | 341 |
| MnlI | CCTC | 5 cut(s) | 35, 47, 118, 204, 253 |
| Mox20I | TGGCCA | 1 cut(s) | 341 |
| Mph1103I | ATGCAT | 1 cut(s) | 136 |
| MscI | TGGCCA | 1 cut(s) | 341 |
| MseI | TTAA | 1 cut(s) | 168 |
| Msp20I | TGGCCA | 1 cut(s) | 341 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 161 |
| NcoI | CCATGG | 1 cut(s) | 240 |
| NdeII | GATC | 1 cut(s) | 70 |
| NlaIII | CATG | 3 cut(s) | 211, 244, 333 |
| NlaIV | GGNNCC | 1 cut(s) | 316 |
| NmuCI | GTSAC | 1 cut(s) | 88 |
| NsiI | ATGCAT | 1 cut(s) | 136 |
| PagI | TCATGA | 1 cut(s) | 207 |
| PkrI | GCNGC | 2 cut(s) | 31, 157 |
| PspN4I | GGNNCC | 1 cut(s) | 316 |
| SaqAI | TTAA | 1 cut(s) | 168 |
| SatI | GCNGC | 2 cut(s) | 30, 156 |
| Sau3AI | GATC | 1 cut(s) | 70 |
| SduI | GDGCHC | 1 cut(s) | 317 |
| SetI | ASST | 6 cut(s) | 19, 27, 39, 109, 181, 328 |
| SfaNI | GCATC | 3 cut(s) | 121, 143, 151 |
| Sse9I | AATT | 1 cut(s) | 203 |
| SspMI | CTAG | 1 cut(s) | 78 |
| StyI | CCWWGG | 2 cut(s) | 240, 255 |
| TaaI | ACNGT | 2 cut(s) | 220, 319 |
| TaqI | TCGA | 1 cut(s) | 116 |
| TasI | AATT | 1 cut(s) | 203 |
| Tru1I | TTAA | 1 cut(s) | 168 |
| Tru9I | TTAA | 1 cut(s) | 168 |
| TscAI | CASTG | 1 cut(s) | 235 |
| TseFI | GTSAC | 1 cut(s) | 88 |
| TseI | GCWGC | 2 cut(s) | 29, 155 |
| Tsp45I | GTSAC | 1 cut(s) | 88 |
| TspDTI | ATGAA | 1 cut(s) | 196 |
| TspGWI | ACGGA | 2 cut(s) | 281, 293 |
| TspRI | CASTG | 1 cut(s) | 235 |
| XapI | RAATTY | 1 cut(s) | 203 |
| XspI | CTAG | 1 cut(s) | 78 |
| Zsp2I | ATGCAT | 1 cut(s) | 136 |