Rh5BG286700

metal ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000343
Physical Location & Seq
Reverse (-)
36487848 .. 36500904
13057 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG286700.1

Sequence Viewer

Length: 2463 bp
ATGGAATCGTCAGATGATGAGGCTGAGGCAATGCAGCTTTCGGTTTCGAATTACTACTTTGTAGATGGCAAGGAAGATCCTATCTCCTTTCATGTACTGCCCATTCAATGGAGTGAGGGTGATAGGCAGGATGGGAAGGAAGCAGTGTTGTTTCTGCATGGCAGTGCCGACAATGGGCTCGAGAAGATTTACAAACCTGTTGTTGCTTGGAAGTTTGATCTTTCGAATGTGAAGCCCGAGATTGCAGTGCTTTCCAAGGAGAATGATTGGATTCTGCTCCAGAAGCCGAGGAGGAGCTATGAGGGGATCGTAAGGACTGTCTTGATAACTGTGCAGTGCCTTAGTTATGCAATGTGGAATGCTGAGGCGTCCGGAAAAGCTGTGTGGGATCACTTGTCTAAAGCGTTCAGCATGTATGAGAACAGGCCTTCGCAGCATGATTTGGGTGATCACAGGAGTCTGATTAGTGAAGCTCTAAAGCGAGATGATGCCTTGGCAAAGTCGAAGTTTATAATTGACTTTCTTAAAGAAGAGCCACCCACAAAGAGGAAACTATCTGATGAGGATATTCAAGCTACAGCAAAGCTTGGATTTATAGTTGATGATGCGGAAGATTATATAATTGATGATGAAGATGAGTCAGATGATGATGATGATGATAACCTGTTTGATTCTGTTTGTGCCTTTTGTGACAATGGTGGCAAGCTGTTATGTTGTGAAGGGAGATGCCTAAGGTCCTTCCATCCCACGGTAGAGGACGGCGAGGACTCAATGTGTGAATCTCTTGGATTCACTCGGGAGGAAGTAGATGCAATGGCAAGTTTCTTCTGTAAGAACTGTCAATATAAGCAGCATCAGTGCTTTGCTTGTGGGAAGTTAGGCTCCTCAGACAAATCTTTGGGTGCAAAGGTCTTCCCCTGTGTTTCTGCAACCTGTGGTCAATTTTACCATCCTCATTGTGTTGCACAACTAATTTATCAAGACAATGGAGTTTCTGCTGAAGAACTGGAGAAAAATATCTCTCTGGGGGAGTCTTTTACGTGTCCGATTCATAAGTGCTGTGTTTGTAAGCAAGGAGAGAATAAGAAGGATCCCGAGTTACGGTTTGCTGTGTGCAGGCGTTGTCCTAAATCCTATCACAGGAAATGCTTGCCCAGTGAGATTCGTTTTGAGAAAACAGAGGAAGATGATGAGGAAGAGATAGAGGATGAAGAAGAAATAGAAACAAGAGCTTGGGAAGGTCTATTACCTAATCGCATACTAATATATTGCACAGAACACGAAATAGATGAAGAAATTGGAACTCCAATAAGGGACCATGTTAAATTTCCCGATGTTGACGGAAAGAAGACTAGTATTGTGAAAAGGAAGACTGCTTTTGAACAGAAAAAGAGAAGACTGACATCAGAGTCCCTTGTGGTCAGAGATAATTCTCTGTCGAAGAAAAGAAAGCTTACCTCAGAGGGATTTTATAGTGGAAGAACTGCACCTCCAATATCTAGGCAGAAATTGAATTCGGGTGAAAAAGTTGGCAGTAGCAGAAATACTGAAAAAGTACCTTCTCGGTTAGACGTATCAAGAAAGGTGATGGTTAATAAGACATTGAAAAAGGAAGTTCAGACTAGCGTGGAAAAGAAGACCTCGTTGGGTAATAGACTGTTTACCTACATAAAGGGATCTGGGTCAGTAAAGTTTGGGAAGCAGGATACACCTGATGATGCTGAACTAAATTCTAAAACGAGTGCAAAAATTGATCCTACATCAAGAACATTGAGTGCGGAAGCTTCCTTAGATCCTGCTAGAGAAAGGAGATTACATGCTTTAATGAAAGACGCTGCTTCATCAATAAGATTGGAAGATGTTATAGAAAAGCACAAAGTTCCATCCACTCATGCATCCTCAAATAAATATGCTGTAGAAAGGAACATAACACGAGGAAAGGTGGAAGGCTCAGTTGAGGCTGTTCGAACCGCATTAAAGAAACTACAGGAAGGCTGCAGTACTGAAGATGCAGAGGCTGTCTGTGCTCCTGAGATTCTTTCCCAGATTTATAAGTGGAAGAGCAAGCTTAGAGTATATCTTGCACCTTTCTTGCATGGAAAGCGGTACACGTCCTTCGGTCGCCATTTCACAAAGGTGGAAAAACTCGAACAGATTGCTGATAGGCTCCATTCGTACGTACAGAATGGAGACACGATAGTGGACTTCTGTTGTGGCTCTAATGATTTCAGCATCGTAATGAAGAAAAAGCTTGAGGAGACAGGGAAGAAGTGCTATTTTAGAAATTATGACATTATTCAACCAAAGAATGATTTCTGTTTTGAGAAGAGAGATTGGATGACTGTTCAAAAAGGTGAATTACCGGATAGTAGTAAATTGGTAAGAAATGGAGGCTTGGTTATATTTTACATCAAGTTGTTTGGATTCCTTAAGCCTTGTGCTAATTGTTTCACTATTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006325 GO:0006355 GO:0006950 GO:0006952 GO:0006996 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0009605 GO:0009607 GO:0009620 GO:0009791 GO:0009889 GO:0009893 GO:0009909 GO:0009911 GO:0009987 GO:0010228 GO:0010468 GO:0010556 GO:0010604 GO:0010638 GO:0016043 GO:0019219 GO:0019222 GO:0022414 GO:0023052 GO:0030154 GO:0031056 GO:0031058 GO:0031060 GO:0031062 GO:0031323 GO:0031325 GO:0031326 GO:0031399 GO:0031401 GO:0031440 GO:0031935 GO:0031937 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033043 GO:0033044 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0043954 GO:0044030 GO:0044424 GO:0044464 GO:0048366 GO:0048367 GO:0048468 GO:0048518 GO:0048522 GO:0048580 GO:0048582 GO:0048608 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0048869 GO:0050684 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051052 GO:0051094 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051276 GO:0051570 GO:0051574 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060968 GO:0061085 GO:0061087 GO:0061458 GO:0065007 GO:0070827 GO:0070828 GO:0070829 GO:0071840 GO:0080090 GO:0090436 GO:0097159 GO:0098542 GO:0099402 GO:0140110 GO:1900109 GO:1900111 GO:1900363 GO:1901363 GO:1902275 GO:1903311 GO:1903506 GO:1905269 GO:2000024 GO:2000026 GO:2000112 GO:2000241 GO:2000243 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNMT1-RFD

Protein Analysis

820

Amino Acids

93.0

Weight (kDa)

6.05

Isoelectric Point (pI)

42.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNMT1-RFD PF12047 13 - 111 1e-06 Cytosine specific DNA methyltransferase replication foci domain
PHD_NSD PF22908 283 - 351 1.8e-19 Histone-lysine N-methyltransferase NSD-like, PHD zinc finger
Mtase_EDM2 PF26055 726 - 794 8.3e-33 EDM2 methyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000343)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48090 AT5G48090 AT5G48090 AT5G55390 AT5G55390 AT5G55390
fragaria_vesca FvH4_3g23690 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160
malus_domestica MD03G1191000.v1.1 MD11G1207800.v1.1 MD11G1208000.v1.1
prunus_persica Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227600_v2.0.a1 Prupe.4G227700_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1
pyrus_communis pycom01g03400 pycom03g14420 pycom11g18100 pycom11g18140 pycom11g18150 pycom11g18160
rosa_chinensis RchiOBHm_Chr2g0145981 RchiOBHm_Chr4g0405241 RchiOBHm_Chr4g0405271 RchiOBHm_Chr5g0016201 RchiOBHm_Chr5g0041831 RchiOBHm_Chr5g0041871 RchiOBHm_Chr5g0041881 RchiOBHm_Chr5g0059241
rosa_laevigata RLG00000002117 RLG00000008861 RLG00000008862 RLG00000011000 RLG00000033216 RLG00000034097 RLG00000034101
rosa_multiflora Rmu_sc0000161.1_g000001 Rmu_sc0000861.1_g000040 Rmu_sc0007522.1_g000003 Rmu_sc0009443.1_g000015 Rmu_sc0022129.1_g000001 Rmu_sc0042315.1_g000001
rosa_roxburghii Rroxscaffold_1G00038660 Rroxscaffold_1G00038690 Rroxscaffold_5G00349560 Rroxscaffold_5G00349610 Rroxscaffold_6G00388370 Rroxscaffold_7G00188740
rosa_rugosa Rorug03G0199700 Rorug04G0055400 Rorug04G0055500 Rorug04G0055900 Rorug04G0056000 Rorug04G0056100 Rorug04G0056100 Rorug04G0056200 Rorug05G0195600 Rorug05G0195700 Rorug05G0195800 Rorug05G0195900 Rorug05G0196800 Rorug05G0385000
rosa_samantha Rh4AG129900 Rh4AG130100 Rh4BG124200 Rh4BG124400 Rh4BG124500 Rh4CG137200 Rh4CG137300 Rh4DG123500 Rh4DG123700 Rh5AG280800 Rh5AG281400 Rh5BG286700 Rh5BG287000 Rh5BG397800 Rh5CG318100 Rh5CG318600 Rh5CG421600 Rh5DG132300 Rh5DG132400 Rh5DG295400 Rh5DG295700 Rh7BG475300
rosa_wichuraiana Rw0G001330 Rw0G005730 Rw4G008670 Rw4G010510 Rw4G010540 Rw5G026450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 512, 2052
AasI GACNNNNNNGTC 1 cut(s) 1408
AccB7I CCANNNNNTGG 1 cut(s) 108
AccIII TCCGGA 1 cut(s) 371
AciI CCGC 4 cut(s) 608, 1778, 1971, 2104
AclWI GGATC 8 cut(s) 71, 314, 396, 1085, 1098, 1684, 1748, 1787
AcsI RAATTY 3 cut(s) 1325, 1513, 1729
AcuI CTGAAG 2 cut(s) 1020, 2025
AcyI GRCGYC 1 cut(s) 368
AfaI GTAC 6 cut(s) 96, 1557, 2002, 2108, 2177, 2181
AfiI CCNNNNNNNGG 6 cut(s) 108, 174, 546, 748, 1101, 1140
AflII CTTAAG 1 cut(s) 2429
AflIII ACRYGT 2 cut(s) 1040, 2109
AgsI TTSAA 7 cut(s) 107, 572, 1382, 1513, 1606, 2300, 2348
AhlI ACTAGT 1 cut(s) 1352
AjiI CACGTC 1 cut(s) 2112
AjuI GAANNNNNNNTTGG 2 cut(s) 1628, 1660
AleI CACNNNNGTG 2 cut(s) 2135, 2198
AloI GAACNNNNNNTCC 2 cut(s) 1474, 1506
Alw21I GWGCWC 1 cut(s) 2029
Alw26I GTCTC 2 cut(s) 2184, 2252
AlwI GGATC 8 cut(s) 71, 314, 396, 1085, 1098, 1684, 1748, 1787
AlwNI CAGNNNCTG 1 cut(s) 2018
Ama87I CYCGRG 4 cut(s) 179, 236, 795, 1094
Aor13HI TCCGGA 1 cut(s) 371
AoxI GGCC 1 cut(s) 425
ApeKI GCWGC 5 cut(s) 34, 433, 850, 1835, 1995
ApoI RAATTY 3 cut(s) 1325, 1513, 1729
Asp700I GAANNNNTTC 1 cut(s) 2312
AspS9I GGNCC 2 cut(s) 735, 1315
AsuHPI GGTGA 5 cut(s) 131, 458, 1532, 1597, 2366
AsuII TTCGAA 3 cut(s) 47, 224, 1966
AvaI CYCGRG 4 cut(s) 179, 236, 795, 1094
AvaII GGWCC 2 cut(s) 735, 1315
AxyI CCTNAGG 1 cut(s) 731
BamHI GGATCC 1 cut(s) 1090
BanII GRGCYC 1 cut(s) 180
BarI GAAGNNNNNNTAC 4 cut(s) 1230, 1262, 2099, 2131
BauI CACGAG 1 cut(s) 1932
BbsI GAAGAC 5 cut(s) 904, 1355, 1376, 1402, 1643
Bbv12I GWGCWC 1 cut(s) 2029
BbvCI CCTCAGC 2 cut(s) 24, 363
BbvI GCAGC 5 cut(s) 46, 445, 862, 1822, 1982
BccI CCATC 6 cut(s) 59, 125, 750, 957, 1582, 1891
BceAI ACGGC 1 cut(s) 775
BcgI CGANNNNNNTGC 2 cut(s) 2138, 2172
BciVI GTATCC 1 cut(s) 1699
BclI TGATCA 1 cut(s) 448
BcoDI GTCTC 2 cut(s) 2184, 2252
BcuI ACTAGT 1 cut(s) 1352
BfaI CTAG 4 cut(s) 1353, 1500, 1623, 1800
BfmI CTRYAG 4 cut(s) 576, 1914, 1985, 1996
BfrI CTTAAG 1 cut(s) 2429
BfuI GTATCC 1 cut(s) 1699
BisI GCNGC 5 cut(s) 35, 434, 851, 1836, 1996
BlsI GCNGC 5 cut(s) 36, 435, 852, 1837, 1997
BmcAI AGTACT 1 cut(s) 2002
Bme18I GGWCC 2 cut(s) 735, 1315
BmeT110I CYCGRG 4 cut(s) 179, 236, 795, 1094
BmgBI CACGTC 1 cut(s) 2112
BmgT120I GGNCC 2 cut(s) 735, 1315
BmiI GGNNCC 4 cut(s) 883, 1092, 1316, 2168
BmrI ACTGGG 1 cut(s) 1149
BmsI GCATC 9 cut(s) 478, 595, 716, 799, 862, 1708, 1904, 1999, 2241
BmuI ACTGGG 1 cut(s) 1149
BpiI GAAGAC 5 cut(s) 904, 1355, 1376, 1402, 1643
BpmI CTGGAG 2 cut(s) 263, 1028
Bpu10I CCTNAGC 2 cut(s) 24, 363
Bpu14I TTCGAA 3 cut(s) 47, 224, 1966
BpuEI CTTGAG 1 cut(s) 2273
BsaAI YACGTR 2 cut(s) 1041, 2179
BsaHI GRCGYC 1 cut(s) 368
BsaJI CCNNGG 4 cut(s) 255, 287, 492, 747
BsaWI WCCGGW 2 cut(s) 371, 2362
BsaXI ACNNNNNCTCC 6 cut(s) 103, 133, 1474, 1504, 2382, 2412
Bsc4I CCNNNNNNNGG 6 cut(s) 108, 174, 546, 748, 1101, 1140
Bse1I ACTGG 2 cut(s) 1011, 1155
Bse21I CCTNAGG 1 cut(s) 731
Bse3DI GCAATG 3 cut(s) 36, 357, 819
BseAI TCCGGA 1 cut(s) 371
BseDI CCNNGG 4 cut(s) 255, 287, 492, 747
BseGI GGATG 7 cut(s) 136, 742, 949, 1213, 1883, 1895, 2343
BseLI CCNNNNNNNGG 6 cut(s) 108, 174, 546, 748, 1101, 1140
BseMI GCAATG 3 cut(s) 36, 357, 819
BseMII CTCAG 6 cut(s) 15, 354, 900, 1473, 1965, 2022
BseNI ACTGG 2 cut(s) 1011, 1155
BseRI GAGGAG 4 cut(s) 304, 307, 874, 2270
BseXI GCAGC 5 cut(s) 46, 445, 862, 1822, 1982
BsgI GTGCAG 3 cut(s) 353, 1135, 1470
Bsh1285I CGRYCG 1 cut(s) 2122
BshFI GGCC 1 cut(s) 427
BsiEI CGRYCG 1 cut(s) 2122
BsiHKAI GWGCWC 1 cut(s) 2029
BsiHKCI CYCGRG 4 cut(s) 179, 236, 795, 1094
BsiSI CCGG 2 cut(s) 372, 2363
BsiWI CGTACG 1 cut(s) 2175
BslFI GGGAC 2 cut(s) 1328, 1396
BslI CCNNNNNNNGG 6 cut(s) 108, 174, 546, 748, 1101, 1140
BsmAI GTCTC 2 cut(s) 2184, 2252
BsmFI GGGAC 2 cut(s) 1328, 1396
BsmI GAATGC 1 cut(s) 364
BsnI GGCC 1 cut(s) 427
BsoBI CYCGRG 4 cut(s) 179, 236, 795, 1094
Bsp119I TTCGAA 3 cut(s) 47, 224, 1966
Bsp1286I GDGCHC 2 cut(s) 180, 2029
Bsp13I TCCGGA 1 cut(s) 371
Bsp143I GATC 9 cut(s) 76, 217, 306, 388, 448, 1090, 1676, 1753, 1792
BspACI CCGC 4 cut(s) 608, 1778, 1971, 2104
BspANI GGCC 1 cut(s) 427
BspCNI CTCAG 6 cut(s) 16, 355, 899, 1472, 1964, 2023
BspEI TCCGGA 1 cut(s) 371
BspLI GGNNCC 4 cut(s) 883, 1092, 1316, 2168
BspMAI CTGCAG 1 cut(s) 2000
BspPI GGATC 8 cut(s) 71, 314, 396, 1085, 1098, 1684, 1748, 1787
BspQI GCTCTTC 2 cut(s) 525, 2054
BspT104I TTCGAA 3 cut(s) 47, 224, 1966
BspTI CTTAAG 1 cut(s) 2429
BsrDI GCAATG 3 cut(s) 36, 357, 819
BsrI ACTGG 2 cut(s) 1011, 1155
BssECI CCNNGG 4 cut(s) 255, 287, 492, 747
BssMI GATC 9 cut(s) 76, 217, 306, 388, 448, 1090, 1676, 1753, 1792
BssNI GRCGYC 1 cut(s) 368
BssSI CACGAG 1 cut(s) 1932
BssT1I CCWWGG 2 cut(s) 255, 492
Bst2BI CACGAG 1 cut(s) 1932
Bst4CI ACNGT 7 cut(s) 319, 331, 751, 839, 1104, 1659, 2344
Bst6I CTCTTC 4 cut(s) 525, 1191, 2054, 2321
BstACI GRCGYC 1 cut(s) 368
BstAFI CTTAAG 1 cut(s) 2429
BstBAI YACGTR 2 cut(s) 1041, 2179
BstBI TTCGAA 3 cut(s) 47, 224, 1966
BstC8I GCNNGC 4 cut(s) 704, 1118, 1151, 2066
BstDSI CCRYGG 1 cut(s) 747
BstENI CCTNNNNNAGG 1 cut(s) 1138
BstF5I GGATG 7 cut(s) 136, 742, 949, 1213, 1883, 1895, 2343
BstKTI GATC 9 cut(s) 79, 220, 309, 391, 451, 1093, 1679, 1756, 1795
BstMAI GTCTC 2 cut(s) 2184, 2252
BstMBI GATC 9 cut(s) 76, 217, 306, 388, 448, 1090, 1676, 1753, 1792
BstMCI CGRYCG 1 cut(s) 2122
BstMWI GCNNNNNNNGC 4 cut(s) 283, 433, 2024, 2101
BstNSI RCATGY 2 cut(s) 415, 1820
BstSFI CTRYAG 4 cut(s) 576, 1914, 1985, 1996
BstSNI TACGTA 1 cut(s) 2179
BstV1I GCAGC 5 cut(s) 46, 445, 862, 1822, 1982
BstV2I GAAGAC 5 cut(s) 904, 1355, 1376, 1402, 1643
BstX2I RGATCY 4 cut(s) 76, 1090, 1676, 1792
BstYI RGATCY 4 cut(s) 76, 1090, 1676, 1792
Bsu36I CCTNAGG 1 cut(s) 731
BsuI GTATCC 1 cut(s) 1699
BsuRI GGCC 1 cut(s) 427
BtgI CCRYGG 1 cut(s) 747
BtrI CACGTC 1 cut(s) 2112
BtsCI GGATG 7 cut(s) 136, 742, 949, 1213, 1883, 1895, 2343
BtsI GCAGTG 4 cut(s) 150, 169, 252, 341
BtsIMutI CAGTG 6 cut(s) 150, 169, 252, 341, 863, 1162
Cac8I GCNNGC 4 cut(s) 704, 1118, 1151, 2066
CaiI CAGNNNCTG 1 cut(s) 2018
Cfr13I GGNCC 2 cut(s) 735, 1315
CseI GACGC 2 cut(s) 357, 1841
Csp6I GTAC 6 cut(s) 95, 1556, 2001, 2107, 2176, 2180
CviAII CATG 8 cut(s) 92, 158, 412, 437, 1319, 1817, 1892, 2096
CviQI GTAC 6 cut(s) 95, 1556, 2001, 2107, 2176, 2180
DpnI GATC 9 cut(s) 78, 219, 308, 390, 450, 1092, 1678, 1755, 1794
DpnII GATC 9 cut(s) 76, 217, 306, 388, 448, 1090, 1676, 1753, 1792
DrdI GACNNNNNNGTC 1 cut(s) 1408
DseDI GACNNNNNNGTC 1 cut(s) 1408
Eam1104I CTCTTC 4 cut(s) 525, 1191, 2054, 2321
EarI CTCTTC 4 cut(s) 525, 1191, 2054, 2321
Eco105I TACGTA 1 cut(s) 2179
Eco130I CCWWGG 2 cut(s) 255, 492
Eco147I AGGCCT 1 cut(s) 427
Eco24I GRGCYC 1 cut(s) 180
Eco47I GGWCC 2 cut(s) 735, 1315
Eco57I CTGAAG 2 cut(s) 1020, 2025
Eco81I CCTNAGG 1 cut(s) 731
Eco88I CYCGRG 4 cut(s) 179, 236, 795, 1094
EcoNI CCTNNNNNAGG 1 cut(s) 1138
EcoO109I RGGNCCY 1 cut(s) 735
EcoRI GAATTC 1 cut(s) 1513
EcoT14I CCWWGG 2 cut(s) 255, 492
EcoT22I ATGCAT 1 cut(s) 1897
EcoT38I GRGCYC 1 cut(s) 180
ErhI CCWWGG 2 cut(s) 255, 492
FaeI CATG 8 cut(s) 95, 161, 415, 440, 1322, 1820, 1895, 2099
FaqI GGGAC 2 cut(s) 1328, 1396
FatI CATG 8 cut(s) 91, 157, 411, 436, 1318, 1816, 1891, 2095
FbaI TGATCA 1 cut(s) 448
Fnu4HI GCNGC 5 cut(s) 35, 434, 851, 1836, 1996
FokI GGATG 7 cut(s) 143, 729, 936, 1220, 1870, 1882, 2350
FriOI GRGCYC 1 cut(s) 180
Fsp4HI GCNGC 5 cut(s) 35, 434, 851, 1836, 1996
FspBI CTAG 4 cut(s) 1353, 1500, 1623, 1800
GluI GCNGC 5 cut(s) 35, 434, 851, 1836, 1996
GsuI CTGGAG 2 cut(s) 263, 1028
HaeIII GGCC 1 cut(s) 427
HapII CCGG 2 cut(s) 372, 2363
HgaI GACGC 2 cut(s) 357, 1841
Hin1I GRCGYC 1 cut(s) 368
Hin1II CATG 8 cut(s) 95, 161, 415, 440, 1322, 1820, 1895, 2099
HincII GTYRAC 1 cut(s) 1339
HindII GTYRAC 1 cut(s) 1339
HindIII AAGCTT 5 cut(s) 584, 1451, 1782, 2066, 2249
HpaII CCGG 2 cut(s) 372, 2363
HphI GGTGA 5 cut(s) 131, 458, 1532, 1597, 2366
Hpy166II GTNNAC 4 cut(s) 1339, 1662, 2109, 2203
Hpy8I GTNNAC 4 cut(s) 1339, 1662, 2109, 2203
HpyCH4III ACNGT 7 cut(s) 319, 331, 751, 839, 1104, 1659, 2344
HpyCH4IV ACGT 4 cut(s) 1040, 1572, 2111, 2178
HpyF10VI GCNNNNNNNGC 4 cut(s) 283, 433, 2024, 2101
HpySE526I ACGT 4 cut(s) 1040, 1572, 2111, 2178
Hsp92I GRCGYC 1 cut(s) 368
Hsp92II CATG 8 cut(s) 95, 161, 415, 440, 1322, 1820, 1895, 2099
Kpn2I TCCGGA 1 cut(s) 371
Ksp22I TGATCA 1 cut(s) 448
Kzo9I GATC 9 cut(s) 76, 217, 306, 388, 448, 1090, 1676, 1753, 1792
LguI GCTCTTC 2 cut(s) 525, 2054
LmnI GCTCC 5 cut(s) 282, 294, 887, 2032, 2172
Lsp1109I GCAGC 5 cut(s) 46, 445, 862, 1822, 1982
LweI GCATC 9 cut(s) 478, 595, 716, 799, 862, 1708, 1904, 1999, 2241
MaeI CTAG 4 cut(s) 1353, 1500, 1623, 1800
MaeII ACGT 4 cut(s) 1040, 1572, 2111, 2178
MaeIII GTNAC 2 cut(s) 689, 1098
MalI GATC 9 cut(s) 78, 219, 308, 390, 450, 1092, 1678, 1755, 1794
MboI GATC 9 cut(s) 76, 217, 306, 388, 448, 1090, 1676, 1753, 1792
MflI RGATCY 4 cut(s) 76, 1090, 1676, 1792
MhlI GDGCHC 2 cut(s) 180, 2029
MlyI GAGTC 5 cut(s) 466, 647, 761, 1040, 1418
Mph1103I ATGCAT 1 cut(s) 1897
MroI TCCGGA 1 cut(s) 371
MroXI GAANNNNTTC 1 cut(s) 2312
MseI TTAA 6 cut(s) 525, 1323, 1593, 1823, 1976, 2430
MslI CAYNNNNRTG 4 cut(s) 162, 2135, 2198, 2237
MspCI CTTAAG 1 cut(s) 2429
MspI CCGG 2 cut(s) 372, 2363
Mva1269I GAATGC 1 cut(s) 364
MwoI GCNNNNNNNGC 4 cut(s) 283, 433, 2024, 2101
NdeII GATC 9 cut(s) 76, 217, 306, 388, 448, 1090, 1676, 1753, 1792
NlaIII CATG 8 cut(s) 95, 161, 415, 440, 1322, 1820, 1895, 2099
NlaIV GGNNCC 4 cut(s) 883, 1092, 1316, 2168
NmeAIII GCCGAG 1 cut(s) 312
NmuCI GTSAC 1 cut(s) 689
NsiI ATGCAT 1 cut(s) 1897
NspI RCATGY 2 cut(s) 415, 1820
NspV TTCGAA 3 cut(s) 47, 224, 1966
OliI CACNNNNGTG 2 cut(s) 2135, 2198
PaeR7I CTCGAG 1 cut(s) 179
PceI AGGCCT 1 cut(s) 427
PciSI GCTCTTC 2 cut(s) 525, 2054
PctI GAATGC 1 cut(s) 364
PdmI GAANNNNTTC 1 cut(s) 2312
PfeI GAWTC 9 cut(s) 5, 271, 671, 779, 789, 1048, 1162, 2035, 2424
Pfl23II CGTACG 1 cut(s) 2175
PflMI CCANNNNNTGG 1 cut(s) 108
PkrI GCNGC 5 cut(s) 36, 435, 852, 1837, 1997
PleI GAGTC 5 cut(s) 465, 646, 761, 1039, 1417
PpsI GAGTC 5 cut(s) 465, 646, 761, 1039, 1417
Ppu21I YACGTR 2 cut(s) 1041, 2179
PpuMI RGGWCCY 1 cut(s) 735
PsiI TTATAA 2 cut(s) 512, 2052
Psp5II RGGWCCY 1 cut(s) 735
PspLI CGTACG 1 cut(s) 2175
PspN4I GGNNCC 4 cut(s) 883, 1092, 1316, 2168
PspPI GGNCC 2 cut(s) 735, 1315
PspPPI RGGWCCY 1 cut(s) 735
PstI CTGCAG 1 cut(s) 2000
PstNI CAGNNNCTG 1 cut(s) 2018
PsuI RGATCY 4 cut(s) 76, 1090, 1676, 1792
RsaI GTAC 6 cut(s) 96, 1557, 2002, 2108, 2177, 2181
RsaNI GTAC 6 cut(s) 95, 1556, 2001, 2107, 2176, 2180
RseI CAYNNNNRTG 4 cut(s) 162, 2135, 2198, 2237
SapI GCTCTTC 2 cut(s) 525, 2054
SaqAI TTAA 6 cut(s) 525, 1323, 1593, 1823, 1976, 2430
SatI GCNGC 5 cut(s) 35, 434, 851, 1836, 1996
Sau3AI GATC 9 cut(s) 76, 217, 306, 388, 448, 1090, 1676, 1753, 1792
Sau96I GGNCC 2 cut(s) 735, 1315
ScaI AGTACT 1 cut(s) 2002
SchI GAGTC 5 cut(s) 466, 647, 761, 1040, 1418
SduI GDGCHC 2 cut(s) 180, 2029
SfaNI GCATC 9 cut(s) 478, 595, 716, 799, 862, 1708, 1904, 1999, 2241
SfcI CTRYAG 4 cut(s) 576, 1914, 1985, 1996
Sfr274I CTCGAG 1 cut(s) 179
SfuI TTCGAA 3 cut(s) 47, 224, 1966
SinI GGWCC 2 cut(s) 735, 1315
SlaI CTCGAG 1 cut(s) 179
SmiMI CAYNNNNRTG 4 cut(s) 162, 2135, 2198, 2237
SmlI CTYRAG 3 cut(s) 179, 2252, 2429
SmoI CTYRAG 3 cut(s) 179, 2252, 2429
SnaBI TACGTA 1 cut(s) 2179
SpeI ACTAGT 1 cut(s) 1352
SseBI AGGCCT 1 cut(s) 427
SsiI CCGC 4 cut(s) 608, 1778, 1971, 2104
SspMI CTAG 4 cut(s) 1353, 1500, 1623, 1800
StuI AGGCCT 1 cut(s) 427
StyI CCWWGG 2 cut(s) 255, 492
TaaI ACNGT 7 cut(s) 319, 331, 751, 839, 1104, 1659, 2344
TaiI ACGT 4 cut(s) 1043, 1575, 2114, 2181
TaqI TCGA 7 cut(s) 47, 180, 224, 503, 1439, 1966, 2148
TaqII GACCGA 1 cut(s) 2108
TatI WGTACW 2 cut(s) 94, 2000
TfiI GAWTC 9 cut(s) 5, 271, 671, 779, 789, 1048, 1162, 2035, 2424
Tru1I TTAA 6 cut(s) 525, 1323, 1593, 1823, 1976, 2430
Tru9I TTAA 6 cut(s) 525, 1323, 1593, 1823, 1976, 2430
TscAI CASTG 6 cut(s) 150, 169, 252, 341, 863, 1162
TseFI GTSAC 1 cut(s) 689
TseI GCWGC 5 cut(s) 34, 433, 850, 1835, 1995
Tsp45I GTSAC 1 cut(s) 689
TspDTI ATGAA 8 cut(s) 80, 645, 1040, 1224, 1305, 1830, 1841, 2255
TspGWI ACGGA 1 cut(s) 1356
TspRI CASTG 6 cut(s) 150, 169, 252, 341, 863, 1162
Van91I CCANNNNNTGG 1 cut(s) 108
Vha464I CTTAAG 1 cut(s) 2429
VpaK11BI GGWCC 2 cut(s) 735, 1315
XagI CCTNNNNNAGG 1 cut(s) 1138
XapI RAATTY 3 cut(s) 1325, 1513, 1729
XceI RCATGY 2 cut(s) 415, 1820
XhoI CTCGAG 1 cut(s) 179
XmnI GAANNNNTTC 1 cut(s) 2312
XspI CTAG 4 cut(s) 1353, 1500, 1623, 1800
ZrmI AGTACT 1 cut(s) 2002
Zsp2I ATGCAT 1 cut(s) 1897
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.