RchiOBHm_Chr5g0041831

metal ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000343
Physical Location & Seq
Reverse (-)
36696609 .. 36708213
11605 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ32026

Sequence Viewer

Length: 3735 bp
ATGGAATCGTCAGATGATGAGGCTGAGGCAATGCAGCTTTCGGTTTCGAATTACTACTTTGTAGATGGCAAGGAAGATCCTATCTCCTTTCATGTACTGCCCATTCAATGGAGTGAGGGTGATAGGCAGGATGGGAAGGAAGCAGTGTTGTTTCTGCATGGCAGTGCCGACAATGGGCTCGAGAAGATTTACAAACCTGTTGTTGCTTGGAAGTTTGATCTTTCGAATGTGAAGCCCGAGATTGCAGTGCTTTCCAAGGAGAATGATTGGATTCTGCTCCAGAAGCCGAGGAGGAGCTATGAGGGGATCGTAAGGACTGTCTTGATAACTGTGCAGTGCCTTAGTTATGCAATGTGGAATGCTGAGGCGTCCGGAAAAGCTGTGTGGGATCACTTGTCTAAAGCGTTCAGCATGTATGAGAACAGGCCTTCGCAGCATGATTTGGGTGATCACAGGAGTCTGATTAGTGAAGCTCTAAAGCGAGATGATGCCTTGGCAAAGTCGAAGTTTATAATTGACTTTCTTAAAGAAGAGCCACCCACAAAGAGGAAACTATCTGATGAGGATATTCAAGCTACAGCAAAGCTTGGATTTATAGTTGATGATGCGGAAGATTATATAATTGATGATGAAGATGAGTCAGATGATGATGATGATGATAACCTGTTTGATTCTGTTTGTGCCTTTTGTGACAATGGTGGCAAGCTGTTATGTTGTGAAGGGAGATGCCTAAGGTCCTTCCATCCCACGGTAGAGGACGGCGAGGACTCAATGTGTGAATCTCTTGGATTCACTCGGGAGGAAGTAGATGCAATGGCAAGTTTCTTCTGTAAGAACTGTCAATATAAGCAGCATCAGTGCTTTGCTTGTGGGAAGTTAGGCTCCTCAGACAAATCTTTGGGTGCAAAGGTCTTCCCCTGTGTTTCTGCAACCTGTGGTCAATTTTACCATCCTCATTGTGTTGCACAACTAATTTATCAAGACAATGGAGTTTCTGCTGAAGAACTGGAGAAAAATATCTCTCTGGGGGAGTCTTTTACGTGTCCGATTCATAAGTGCTGTGTTTGTAAGCAAGGAGAGAATAAGAAGGATCCCGAGTTACGGTTTGCTGTGTGCAGGCGTTGTCCTAAATCCTATCACAGGAAATGCTTGCCCAGTGAGATTCGTTTTGAGAAAACAGAGGAAGATGATGAGGAAGAGATAGAGGATGAAGAAGAAATAGAAACAAGAGCTTGGGAAGGTCTATTACCTAATCGCATACTAATATATTGCACAGAACACGAAATAGATGAAGAAATTGGAACTCCAATAAGGGACCATGTTAAATTTCCCGATGTTGACGGAAAGAAGACTAGTATTGTGAAAAGGAAGACTGCTTTTGAACAGAAAAAGAGAAGACTGACATCAGAGTCCCTTGTGGTCAGAGATAATTCTCTGTCGAAGAAAAGAAAGCTTACCTCAGAGGGATTTTATAGTGGAAGAACTGCACCTCCAATATCTAGGCAGAAATTGAATTCGGGTGAAAAAGTTGGCAGTAGCAGAAATACTGAAAAAGTACCTTCTCGGTTAGACGTATCAAGAAAGGTGATGGTTAATAAGACATTGAAAAAGGAAGTTCAGACTAGCGTGGAAAAGAAGACCTCGTTGGGTAATAGACTGTTTACCTACATAAAGGGATCTGGGTCAGTAAAGTTTGGGAAGCAGGATACACCTGATGATGCTGAACTAAATTCTAAAACGAGTGCAAAAATTGATCCTACATCAAGAACATTGAGTGCGGAAGCTTCCTTAGATCCTGCTAGAGAAAGGAGATTACATGCTTTAATGAAAGACGCTGCTTCATCAATAAGATTGGAAGATGTTATAGAAAAGCACAAAGTTCCATCCACTCATGCATCCTCAAATAAATATGCTGTAGAAAGGAACATAACACGAGGAAAGGTGGAAGGCTCAGTTGAGGCTGTTCGAACCGCATTAAAGAAACTACAGGAAGGCTGCAGTACTGAAGATGCAGAGGCTGTCTGTGCTCCTGAGATTCTTTCCCAGATTTATAAGTGGAAGAGCAAGCTTAGAGTATATCTTGCACCTTTCTTGCATGGAAAGCGGTACACGTCCTTCGGTCGCCATTTCACAAAGGTGGAAAAACTCGAACAGATTGCTGATAGGCTCCATTCGTACGTACAGAATGGAGACACGATAGTGGACTTCTGTTGTGGCTCTAATGATTTCAGCATCGTAATGAAGAAAAAGCTTGAGGAGACAGGGAAGAAGTGCTATTTTAGAAATTATGACATTATTCAACCAAAGAATGATTTCTGTTTTGAGAAGAGAGATTGGATGACTGTTCAAAAAGGTGAATTACCGGATAGTAGTAAATTGATCATGGGGTTGAACCCTCCTTTTGGGGTTAAAGCATCTTTGGCAAACCAGTTTATTGATAAGGCTCTGCAGTTCCAGCCGAAGCTCCTTATTCTTATTGTTCCACCAGAAACAAAAGGGTTACAATGTTATGATCTGATATGGAAGGATGAACGGTCGCTGTCGGGAAAGTCCTTTTATCTTCCTGGATCTGTTGATGAGAATGACAAGCAAATGGATCAATGGAATGTGACGACACCTCCACTCTATCTTTGGAGTCGTCCTGATTGGTCTGAGAGACACAGAGCTATTGCTCAAAAGGATAGTCACGGTTCCATGTCAGGGTCGGTGGAAGATGGTCATTCAGTTGAGAATAAGGATGAAAATGTTGAAAATCTCATGTTGAAAAATGCTTATCTGACACCGACCGGTAATTCCAGAGATTTTGTTGGTGGGGCAGTCGCAGGCGAGGGACATGAAGATAGATCCGCCAAGGGCAATGATGACAGAGGTTACCATGCGAGTTCTGGTCACCAGAAGAACCAGGTTAGTAAGATTTCTGAGAGGCCGCGGTGTGGTGGCAGAAAAAAGAATGGGAGTGGAGTGGTTGAGTTGTCGCCAGACAACAAATGTGATGGTGGCAATGTTTCTAGTGAGATTCAGATGGAAAGGTCTCCATCTACTGAGCGACAGCGGAAAGCAAATCAGCAGTCTGAGCCCAGCGATTCTGGTTCAAGTATGCATTTCAGAACTGCTTGTGGGAGGACCACAGCCAGCATCCCTGATGACACTGGCAGCAATGTCACAAGCAGTGCTGACCCTTATTCAAGTGTGACTCATAGATGGTCCAGTGGTGCCAGTCCTAGCTCTATATATAGGGATACAAAATTGGAGGAACCTGTTTTGGGTTACATGAGAGAGACAGATAACTTCGGTTATAGACCGTATTCCATGGGGATGGAAGACCCATATAGGAGAGACTCTGATATACGCGCACAAGTTCGCCATTACGGGCAGCAAGATCCTGATATGTTCAGAAGTAATTATCTAGCAGGTCAGGATTTGTTACGTGGTCAGGTTGGATCATTTTCGTCCACGTATGGCCATGCTCATCTAGGTTCAGCAGTTGACTCATCTTACAGAATGAACATGTCAGCCATGCAGCGATATGAACCTCGGCTGGATGAATTGAACCACACAAGAGCGAGTGCCTTAGGAACCCAACCAGCGTTTGGGTATGATCCACGCACATACAGTAGCAACATGTATGATCCCAGGGCACCCCGACCTGGTGACCAAGGGGGCCCCTATGGTTTTGCTCCCGGTTCTCACCAAAGTTTCTCAAACCGGAATTCAGCAGGTTGGCTGAACGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006325 GO:0006355 GO:0006950 GO:0006952 GO:0006996 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0009605 GO:0009607 GO:0009620 GO:0009791 GO:0009889 GO:0009893 GO:0009909 GO:0009911 GO:0009987 GO:0010228 GO:0010468 GO:0010556 GO:0010604 GO:0010638 GO:0016043 GO:0019219 GO:0019222 GO:0022414 GO:0023052 GO:0030154 GO:0031056 GO:0031058 GO:0031060 GO:0031062 GO:0031323 GO:0031325 GO:0031326 GO:0031399 GO:0031401 GO:0031440 GO:0031935 GO:0031937 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033043 GO:0033044 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0043954 GO:0044030 GO:0044424 GO:0044464 GO:0048366 GO:0048367 GO:0048468 GO:0048518 GO:0048522 GO:0048580 GO:0048582 GO:0048608 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0048869 GO:0050684 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051052 GO:0051094 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051276 GO:0051570 GO:0051574 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060968 GO:0061085 GO:0061087 GO:0061458 GO:0065007 GO:0070827 GO:0070828 GO:0070829 GO:0071840 GO:0080090 GO:0090436 GO:0097159 GO:0098542 GO:0099402 GO:0140110 GO:1900109 GO:1900111 GO:1900363 GO:1901363 GO:1902275 GO:1903311 GO:1903506 GO:1905269 GO:2000024 GO:2000026 GO:2000112 GO:2000241 GO:2000243 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNMT1-RFD

Protein Analysis

1244

Amino Acids

139.87

Weight (kDa)

6.01

Isoelectric Point (pI)

44.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNMT1-RFD PF12047 13 - 111 1.8e-06 Cytosine specific DNA methyltransferase replication foci domain
PHD_NSD PF22908 283 - 351 3.1e-19 Histone-lysine N-methyltransferase NSD-like, PHD zinc finger
Mtase_EDM2 PF26055 726 - 892 4.1e-88 EDM2 methyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000343)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48090 AT5G48090 AT5G48090 AT5G55390 AT5G55390 AT5G55390
fragaria_vesca FvH4_3g23690 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160
malus_domestica MD03G1191000.v1.1 MD11G1207800.v1.1 MD11G1208000.v1.1
prunus_persica Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227600_v2.0.a1 Prupe.4G227700_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1
pyrus_communis pycom01g03400 pycom03g14420 pycom11g18100 pycom11g18140 pycom11g18150 pycom11g18160
rosa_chinensis RchiOBHm_Chr2g0145981 RchiOBHm_Chr4g0405241 RchiOBHm_Chr4g0405271 RchiOBHm_Chr5g0016201 RchiOBHm_Chr5g0041831 RchiOBHm_Chr5g0041871 RchiOBHm_Chr5g0041881 RchiOBHm_Chr5g0059241
rosa_laevigata RLG00000002117 RLG00000008861 RLG00000008862 RLG00000011000 RLG00000033216 RLG00000034097 RLG00000034101
rosa_multiflora Rmu_sc0000161.1_g000001 Rmu_sc0000861.1_g000040 Rmu_sc0007522.1_g000003 Rmu_sc0009443.1_g000015 Rmu_sc0022129.1_g000001 Rmu_sc0042315.1_g000001
rosa_roxburghii Rroxscaffold_1G00038660 Rroxscaffold_1G00038690 Rroxscaffold_5G00349560 Rroxscaffold_5G00349610 Rroxscaffold_6G00388370 Rroxscaffold_7G00188740
rosa_rugosa Rorug03G0199700 Rorug04G0055400 Rorug04G0055500 Rorug04G0055900 Rorug04G0056000 Rorug04G0056100 Rorug04G0056100 Rorug04G0056200 Rorug05G0195600 Rorug05G0195700 Rorug05G0195800 Rorug05G0195900 Rorug05G0196800 Rorug05G0385000
rosa_samantha Rh4AG129900 Rh4AG130100 Rh4BG124200 Rh4BG124400 Rh4BG124500 Rh4CG137200 Rh4CG137300 Rh4DG123500 Rh4DG123700 Rh5AG280800 Rh5AG281400 Rh5BG286700 Rh5BG287000 Rh5BG397800 Rh5CG318100 Rh5CG318600 Rh5CG421600 Rh5DG132300 Rh5DG132400 Rh5DG295400 Rh5DG295700 Rh7BG475300
rosa_wichuraiana Rw0G001330 Rw0G005730 Rw4G008670 Rw4G010510 Rw4G010540 Rw5G026450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 512, 2052
AasI GACNNNNNNGTC 1 cut(s) 1408
Acc36I ACCTGC 2 cut(s) 3401, 3707
AccB1I GGYRCC 2 cut(s) 3212, 3637
AccB7I CCANNNNNTGG 2 cut(s) 108, 3590
AccII CGCG 2 cut(s) 2929, 3351
AccIII TCCGGA 1 cut(s) 371
AciI CCGC 8 cut(s) 608, 1778, 1971, 2104, 2847, 2927, 2929, 3052
AcoI YGGCCR 1 cut(s) 3460
AcsI RAATTY 4 cut(s) 1325, 1513, 1729, 3709
AcuI CTGAAG 2 cut(s) 1020, 2025
AcyI GRCGYC 1 cut(s) 368
AfaI GTAC 6 cut(s) 96, 1557, 2002, 2108, 2177, 2181
AflIII ACRYGT 4 cut(s) 1040, 2109, 3507, 3621
AgeI ACCGGT 1 cut(s) 2786
AhlI ACTAGT 1 cut(s) 1352
AjiI CACGTC 1 cut(s) 2112
AjnI CCWGG 4 cut(s) 2563, 2901, 3632, 3646
AjuI GAANNNNNNNTTGG 2 cut(s) 1628, 1660
AleI CACNNNNGTG 2 cut(s) 2135, 2198
AloI GAACNNNNNNTCC 2 cut(s) 1474, 1506
Alw21I GWGCWC 1 cut(s) 2029
Alw26I GTCTC 6 cut(s) 2184, 2252, 2650, 3036, 3272, 3330
AlwNI CAGNNNCTG 1 cut(s) 2018
Ama87I CYCGRG 4 cut(s) 179, 236, 795, 1094
Aor13HI TCCGGA 1 cut(s) 371
AoxI GGCC 4 cut(s) 425, 2924, 3460, 3661
ApaI GGGCCC 1 cut(s) 3665
ApeKI GCWGC 8 cut(s) 34, 433, 850, 1835, 1995, 3153, 3373, 3520
ApoI RAATTY 4 cut(s) 1325, 1513, 1729, 3709
ArsI GACNNNNNNTTYG 4 cut(s) 2668, 2700, 3053, 3085
AsiGI ACCGGT 1 cut(s) 2786
Asp700I GAANNNNTTC 1 cut(s) 2312
AspLEI GCGC 1 cut(s) 3353
AspS9I GGNCC 6 cut(s) 735, 1315, 3123, 3204, 3661, 3662
AsuC2I CCSGG 1 cut(s) 3681
AsuHPI GGTGA 8 cut(s) 131, 458, 1532, 1597, 2366, 2882, 3662, 3680
AsuII TTCGAA 3 cut(s) 47, 224, 1966
AvaI CYCGRG 4 cut(s) 179, 236, 795, 1094
AvaII GGWCC 4 cut(s) 735, 1315, 3123, 3204
AxyI CCTNAGG 2 cut(s) 731, 3571
BaeGI GKGCMC 2 cut(s) 3640, 3665
BalI TGGCCA 1 cut(s) 3462
BamHI GGATCC 1 cut(s) 1090
BanI GGYRCC 2 cut(s) 3212, 3637
BanII GRGCYC 3 cut(s) 180, 3078, 3665
BarI GAAGNNNNNNTAC 4 cut(s) 1230, 1262, 2099, 2131
BauI CACGAG 1 cut(s) 1932
BbsI GAAGAC 6 cut(s) 904, 1355, 1376, 1402, 1643, 3327
Bbv12I GWGCWC 1 cut(s) 2029
BbvCI CCTCAGC 2 cut(s) 24, 363
BbvI GCAGC 8 cut(s) 46, 445, 862, 1822, 1982, 3165, 3385, 3532
BceAI ACGGC 1 cut(s) 775
BcgI CGANNNNNNTGC 2 cut(s) 2138, 2172
BciT130I CCWGG 4 cut(s) 2565, 2903, 3634, 3648
BciVI GTATCC 2 cut(s) 1699, 3232
BclI TGATCA 2 cut(s) 448, 2379
BcnI CCSGG 1 cut(s) 3681
BcoDI GTCTC 6 cut(s) 2184, 2252, 2650, 3036, 3272, 3330
BcuI ACTAGT 1 cut(s) 1352
BfaI CTAG 8 cut(s) 1353, 1500, 1623, 1800, 3009, 3222, 3407, 3473
BfmI CTRYAG 5 cut(s) 576, 1914, 1985, 1996, 2447
BfuAI ACCTGC 2 cut(s) 3401, 3707
BfuI GTATCC 2 cut(s) 1699, 3232
BisI GCNGC 9 cut(s) 35, 434, 851, 1836, 1996, 2927, 3154, 3374, 3521
BlsI GCNGC 9 cut(s) 36, 435, 852, 1837, 1997, 2928, 3155, 3375, 3522
BmcAI AGTACT 1 cut(s) 2002
Bme1390I CCNGG 5 cut(s) 2565, 2903, 3634, 3648, 3681
Bme18I GGWCC 4 cut(s) 735, 1315, 3123, 3204
BmeT110I CYCGRG 4 cut(s) 179, 236, 795, 1094
BmgBI CACGTC 1 cut(s) 2112
BmgT120I GGNCC 6 cut(s) 735, 1315, 3123, 3204, 3661, 3662
BmrFI CCNGG 5 cut(s) 2565, 2903, 3634, 3648, 3681
BmrI ACTGGG 1 cut(s) 1149
BmuI ACTGGG 1 cut(s) 1149
BpiI GAAGAC 6 cut(s) 904, 1355, 1376, 1402, 1643, 3327
BpmI CTGGAG 2 cut(s) 263, 1028
Bpu10I CCTNAGC 2 cut(s) 24, 363
Bpu14I TTCGAA 3 cut(s) 47, 224, 1966
BpuEI CTTGAG 1 cut(s) 2273
BpuMI CCSGG 1 cut(s) 3681
BsaAI YACGTR 4 cut(s) 1041, 2179, 3428, 3456
BsaHI GRCGYC 1 cut(s) 368
BsaI GGTCTC 1 cut(s) 3036
BsaWI WCCGGW 4 cut(s) 371, 2362, 2786, 3705
BsaXI ACNNNNNCTCC 6 cut(s) 103, 133, 1474, 1504, 2602, 2632
Bse118I RCCGGY 1 cut(s) 2786
Bse1I ACTGG 6 cut(s) 1011, 1155, 2428, 3154, 3207, 3216
Bse21I CCTNAGG 2 cut(s) 731, 3571
Bse3DI GCAATG 6 cut(s) 36, 357, 819, 2863, 3007, 3163
BseAI TCCGGA 1 cut(s) 371
BseBI CCWGG 4 cut(s) 2565, 2903, 3634, 3648
BseMI GCAATG 6 cut(s) 36, 357, 819, 2863, 3007, 3163
BseNI ACTGG 6 cut(s) 1011, 1155, 2428, 3154, 3207, 3216
BseRI GAGGAG 4 cut(s) 304, 307, 874, 2270
BseSI GKGCMC 2 cut(s) 3640, 3665
BseXI GCAGC 8 cut(s) 46, 445, 862, 1822, 1982, 3165, 3385, 3532
BseYI CCCAGC 1 cut(s) 3077
BsgI GTGCAG 3 cut(s) 353, 1135, 1470
Bsh1236I CGCG 2 cut(s) 2929, 3351
Bsh1285I CGRYCG 3 cut(s) 2122, 2537, 2787
BshFI GGCC 4 cut(s) 427, 2926, 3462, 3663
BshNI GGYRCC 2 cut(s) 3212, 3637
BshTI ACCGGT 1 cut(s) 2786
BsiEI CGRYCG 3 cut(s) 2122, 2537, 2787
BsiHKAI GWGCWC 1 cut(s) 2029
BsiHKCI CYCGRG 4 cut(s) 179, 236, 795, 1094
BsiSI CCGG 5 cut(s) 372, 2363, 2787, 3681, 3706
BsiWI CGTACG 1 cut(s) 2175
BslFI GGGAC 3 cut(s) 1328, 1396, 2844
BsmAI GTCTC 6 cut(s) 2184, 2252, 2650, 3036, 3272, 3330
BsmFI GGGAC 3 cut(s) 1328, 1396, 2844
BsmI GAATGC 1 cut(s) 364
BsnI GGCC 4 cut(s) 427, 2926, 3462, 3663
Bso31I GGTCTC 1 cut(s) 3036
BsoBI CYCGRG 4 cut(s) 179, 236, 795, 1094
Bsp119I TTCGAA 3 cut(s) 47, 224, 1966
Bsp120I GGGCCC 1 cut(s) 3661
Bsp1286I GDGCHC 5 cut(s) 180, 2029, 3078, 3640, 3665
Bsp13I TCCGGA 1 cut(s) 371
Bsp19I CCATGG 1 cut(s) 3309
BspACI CCGC 8 cut(s) 608, 1778, 1971, 2104, 2847, 2927, 2929, 3052
BspANI GGCC 4 cut(s) 427, 2926, 3462, 3663
BspEI TCCGGA 1 cut(s) 371
BspFNI CGCG 2 cut(s) 2929, 3351
BspMAI CTGCAG 2 cut(s) 2000, 2451
BspMI ACCTGC 2 cut(s) 3401, 3707
BspQI GCTCTTC 2 cut(s) 525, 2054
BspT104I TTCGAA 3 cut(s) 47, 224, 1966
BspT107I GGYRCC 2 cut(s) 3212, 3637
BspTNI GGTCTC 1 cut(s) 3036
BsrDI GCAATG 6 cut(s) 36, 357, 819, 2863, 3007, 3163
BsrFI RCCGGY 1 cut(s) 2786
BsrI ACTGG 6 cut(s) 1011, 1155, 2428, 3154, 3207, 3216
BssAI RCCGGY 1 cut(s) 2786
BssNI GRCGYC 1 cut(s) 368
BssSI CACGAG 1 cut(s) 1932
BssT1I CCWWGG 5 cut(s) 255, 492, 2850, 3309, 3655
Bst2BI CACGAG 1 cut(s) 1932
Bst2UI CCWGG 4 cut(s) 2565, 2903, 3634, 3648
Bst6I CTCTTC 4 cut(s) 525, 1191, 2054, 2321
BstACI GRCGYC 1 cut(s) 368
BstBAI YACGTR 4 cut(s) 1041, 2179, 3428, 3456
BstBI TTCGAA 3 cut(s) 47, 224, 1966
BstC8I GCNNGC 6 cut(s) 704, 1118, 1151, 2066, 2824, 3133
BstDSI CCRYGG 3 cut(s) 747, 2927, 3309
BstEII GGTNACC 3 cut(s) 2870, 2888, 3650
BstENI CCTNNNNNAGG 1 cut(s) 1138
BstFNI CGCG 2 cut(s) 2929, 3351
BstHHI GCGC 1 cut(s) 3353
BstMAI GTCTC 6 cut(s) 2184, 2252, 2650, 3036, 3272, 3330
BstMCI CGRYCG 3 cut(s) 2122, 2537, 2787
BstMWI GCNNNNNNNGC 7 cut(s) 283, 433, 2024, 2101, 2420, 2455, 3073
BstNI CCWGG 4 cut(s) 2565, 2903, 3634, 3648
BstNSI RCATGY 4 cut(s) 415, 1820, 3511, 3625
BstPI GGTNACC 3 cut(s) 2870, 2888, 3650
BstSCI CCNGG 5 cut(s) 2563, 2901, 3632, 3646, 3679
BstSFI CTRYAG 5 cut(s) 576, 1914, 1985, 1996, 2447
BstSLI GKGCMC 2 cut(s) 3640, 3665
BstSNI TACGTA 1 cut(s) 2179
BstUI CGCG 2 cut(s) 2929, 3351
BstV1I GCAGC 8 cut(s) 46, 445, 862, 1822, 1982, 3165, 3385, 3532
BstV2I GAAGAC 6 cut(s) 904, 1355, 1376, 1402, 1643, 3327
BstX2I RGATCY 7 cut(s) 76, 1090, 1676, 1792, 2567, 2843, 3379
BstXI CCANNNNNNTGG 1 cut(s) 3316
BstYI RGATCY 7 cut(s) 76, 1090, 1676, 1792, 2567, 2843, 3379
Bsu36I CCTNAGG 2 cut(s) 731, 3571
BsuI GTATCC 2 cut(s) 1699, 3232
BsuRI GGCC 4 cut(s) 427, 2926, 3462, 3663
BtgI CCRYGG 3 cut(s) 747, 2927, 3309
BtrI CACGTC 1 cut(s) 2112
BtsI GCAGTG 5 cut(s) 150, 169, 252, 341, 3175
BtsIMutI CAGTG 9 cut(s) 150, 169, 252, 341, 863, 1162, 3147, 3175, 3214
BveI ACCTGC 2 cut(s) 3401, 3707
Cac8I GCNNGC 6 cut(s) 704, 1118, 1151, 2066, 2824, 3133
CaiI CAGNNNCTG 1 cut(s) 2018
CfoI GCGC 1 cut(s) 3353
Cfr10I RCCGGY 1 cut(s) 2786
Cfr13I GGNCC 6 cut(s) 735, 1315, 3123, 3204, 3661, 3662
Cfr42I CCGCGG 1 cut(s) 2930
CseI GACGC 2 cut(s) 357, 1841
CsiI ACCWGGT 2 cut(s) 2901, 3646
Csp6I GTAC 6 cut(s) 95, 1556, 2001, 2107, 2176, 2180
CspAI ACCGGT 1 cut(s) 2786
CviQI GTAC 6 cut(s) 95, 1556, 2001, 2107, 2176, 2180
DrdI GACNNNNNNGTC 1 cut(s) 1408
DseDI GACNNNNNNGTC 1 cut(s) 1408
EaeI YGGCCR 1 cut(s) 3460
Eam1104I CTCTTC 4 cut(s) 525, 1191, 2054, 2321
EarI CTCTTC 4 cut(s) 525, 1191, 2054, 2321
EciI GGCGGA 1 cut(s) 2836
Eco105I TACGTA 1 cut(s) 2179
Eco130I CCWWGG 5 cut(s) 255, 492, 2850, 3309, 3655
Eco147I AGGCCT 1 cut(s) 427
Eco24I GRGCYC 3 cut(s) 180, 3078, 3665
Eco31I GGTCTC 1 cut(s) 3036
Eco47I GGWCC 4 cut(s) 735, 1315, 3123, 3204
Eco57I CTGAAG 2 cut(s) 1020, 2025
Eco81I CCTNAGG 2 cut(s) 731, 3571
Eco88I CYCGRG 4 cut(s) 179, 236, 795, 1094
Eco91I GGTNACC 3 cut(s) 2870, 2888, 3650
EcoNI CCTNNNNNAGG 1 cut(s) 1138
EcoO109I RGGNCCY 3 cut(s) 735, 3661, 3662
EcoO65I GGTNACC 3 cut(s) 2870, 2888, 3650
EcoRI GAATTC 2 cut(s) 1513, 3709
EcoRII CCWGG 4 cut(s) 2563, 2901, 3632, 3646
EcoT14I CCWWGG 5 cut(s) 255, 492, 2850, 3309, 3655
EcoT22I ATGCAT 2 cut(s) 1897, 3102
EcoT38I GRGCYC 3 cut(s) 180, 3078, 3665
ErhI CCWWGG 5 cut(s) 255, 492, 2850, 3309, 3655
FaqI GGGAC 3 cut(s) 1328, 1396, 2844
FbaI TGATCA 2 cut(s) 448, 2379
Fnu4HI GCNGC 9 cut(s) 35, 434, 851, 1836, 1996, 2927, 3154, 3374, 3521
FriOI GRGCYC 3 cut(s) 180, 3078, 3665
Fsp4HI GCNGC 9 cut(s) 35, 434, 851, 1836, 1996, 2927, 3154, 3374, 3521
FspBI CTAG 8 cut(s) 1353, 1500, 1623, 1800, 3009, 3222, 3407, 3473
GlaI GCGC 1 cut(s) 3352
GluI GCNGC 9 cut(s) 35, 434, 851, 1836, 1996, 2927, 3154, 3374, 3521
GsaI CCCAGC 1 cut(s) 3081
GsuI CTGGAG 2 cut(s) 263, 1028
HaeIII GGCC 4 cut(s) 427, 2926, 3462, 3663
HapII CCGG 5 cut(s) 372, 2363, 2787, 3681, 3706
HgaI GACGC 2 cut(s) 357, 1841
HhaI GCGC 1 cut(s) 3353
Hin1I GRCGYC 1 cut(s) 368
Hin6I GCGC 1 cut(s) 3351
HinP1I GCGC 1 cut(s) 3351
HincII GTYRAC 2 cut(s) 1339, 3487
HindII GTYRAC 2 cut(s) 1339, 3487
HindIII AAGCTT 5 cut(s) 584, 1451, 1782, 2066, 2249
HpaII CCGG 5 cut(s) 372, 2363, 2787, 3681, 3706
HphI GGTGA 8 cut(s) 131, 458, 1532, 1597, 2366, 2882, 3662, 3680
Hpy166II GTNNAC 6 cut(s) 1339, 1662, 2109, 2203, 3453, 3487
Hpy8I GTNNAC 6 cut(s) 1339, 1662, 2109, 2203, 3453, 3487
HpyCH4IV ACGT 6 cut(s) 1040, 1572, 2111, 2178, 3427, 3455
HpyF10VI GCNNNNNNNGC 7 cut(s) 283, 433, 2024, 2101, 2420, 2455, 3073
HpySE526I ACGT 6 cut(s) 1040, 1572, 2111, 2178, 3427, 3455
Hsp92I GRCGYC 1 cut(s) 368
HspAI GCGC 1 cut(s) 3351
Kpn2I TCCGGA 1 cut(s) 371
Ksp22I TGATCA 2 cut(s) 448, 2379
KspI CCGCGG 1 cut(s) 2930
LguI GCTCTTC 2 cut(s) 525, 2054
LmnI GCTCC 7 cut(s) 282, 294, 887, 2032, 2172, 2469, 3682
Lsp1109I GCAGC 8 cut(s) 46, 445, 862, 1822, 1982, 3165, 3385, 3532
MabI ACCWGGT 2 cut(s) 2901, 3646
MaeI CTAG 8 cut(s) 1353, 1500, 1623, 1800, 3009, 3222, 3407, 3473
MaeII ACGT 6 cut(s) 1040, 1572, 2111, 2178, 3427, 3455
MflI RGATCY 7 cut(s) 76, 1090, 1676, 1792, 2567, 2843, 3379
MhlI GDGCHC 5 cut(s) 180, 2029, 3078, 3640, 3665
MlsI TGGCCA 1 cut(s) 3462
MluNI TGGCCA 1 cut(s) 3462
MlyI GAGTC 9 cut(s) 466, 647, 761, 1040, 1418, 2644, 3187, 3332, 3482
MmeI TCCRAC 1 cut(s) 3418
Mox20I TGGCCA 1 cut(s) 3462
Mph1103I ATGCAT 2 cut(s) 1897, 3102
MroI TCCGGA 1 cut(s) 371
MroXI GAANNNNTTC 1 cut(s) 2312
MscI TGGCCA 1 cut(s) 3462
MseI TTAA 6 cut(s) 525, 1323, 1593, 1823, 1976, 2409
MslI CAYNNNNRTG 5 cut(s) 162, 2135, 2198, 2237, 3314
Msp20I TGGCCA 1 cut(s) 3462
MspA1I CMGCKG 2 cut(s) 2929, 3052
MspI CCGG 5 cut(s) 372, 2363, 2787, 3681, 3706
MspR9I CCNGG 5 cut(s) 2565, 2903, 3634, 3648, 3681
Mva1269I GAATGC 1 cut(s) 364
MvaI CCWGG 4 cut(s) 2565, 2903, 3634, 3648
MvnI CGCG 2 cut(s) 2929, 3351
MwoI GCNNNNNNNGC 7 cut(s) 283, 433, 2024, 2101, 2420, 2455, 3073
NciI CCSGG 1 cut(s) 3681
NcoI CCATGG 1 cut(s) 3309
NmeAIII GCCGAG 2 cut(s) 312, 3514
NmuCI GTSAC 7 cut(s) 689, 2608, 2684, 2888, 3160, 3190, 3650
NsiI ATGCAT 2 cut(s) 1897, 3102
NspI RCATGY 4 cut(s) 415, 1820, 3511, 3625
NspV TTCGAA 3 cut(s) 47, 224, 1966
OliI CACNNNNGTG 2 cut(s) 2135, 2198
PaeR7I CTCGAG 1 cut(s) 179
PasI CCCWGGG 1 cut(s) 3633
PceI AGGCCT 1 cut(s) 427
PciI ACATGT 2 cut(s) 3507, 3621
PciSI GCTCTTC 2 cut(s) 525, 2054
PctI GAATGC 1 cut(s) 364
PdmI GAANNNNTTC 1 cut(s) 2312
Pfl23II CGTACG 1 cut(s) 2175
PflMI CCANNNNNTGG 2 cut(s) 108, 3590
PfoI TCCNGGA 1 cut(s) 2563
PinAI ACCGGT 1 cut(s) 2786
PkrI GCNGC 9 cut(s) 36, 435, 852, 1837, 1997, 2928, 3155, 3375, 3522
PleI GAGTC 9 cut(s) 465, 646, 761, 1039, 1417, 2643, 3187, 3332, 3482
PpsI GAGTC 9 cut(s) 465, 646, 761, 1039, 1417, 2643, 3187, 3332, 3482
Ppu21I YACGTR 4 cut(s) 1041, 2179, 3428, 3456
PpuMI RGGWCCY 1 cut(s) 735
PscI ACATGT 2 cut(s) 3507, 3621
PsiI TTATAA 2 cut(s) 512, 2052
Psp5II RGGWCCY 1 cut(s) 735
Psp6I CCWGG 4 cut(s) 2563, 2901, 3632, 3646
PspEI GGTNACC 3 cut(s) 2870, 2888, 3650
PspFI CCCAGC 1 cut(s) 3077
PspGI CCWGG 4 cut(s) 2563, 2901, 3632, 3646
PspLI CGTACG 1 cut(s) 2175
PspOMI GGGCCC 1 cut(s) 3661
PspPI GGNCC 6 cut(s) 735, 1315, 3123, 3204, 3661, 3662
PspPPI RGGWCCY 1 cut(s) 735
PstI CTGCAG 2 cut(s) 2000, 2451
PstNI CAGNNNCTG 1 cut(s) 2018
PsuI RGATCY 7 cut(s) 76, 1090, 1676, 1792, 2567, 2843, 3379
RsaI GTAC 6 cut(s) 96, 1557, 2002, 2108, 2177, 2181
RsaNI GTAC 6 cut(s) 95, 1556, 2001, 2107, 2176, 2180
RseI CAYNNNNRTG 5 cut(s) 162, 2135, 2198, 2237, 3314
SacII CCGCGG 1 cut(s) 2930
SapI GCTCTTC 2 cut(s) 525, 2054
SaqAI TTAA 6 cut(s) 525, 1323, 1593, 1823, 1976, 2409
SatI GCNGC 9 cut(s) 35, 434, 851, 1836, 1996, 2927, 3154, 3374, 3521
Sau96I GGNCC 6 cut(s) 735, 1315, 3123, 3204, 3661, 3662
ScaI AGTACT 1 cut(s) 2002
SchI GAGTC 9 cut(s) 466, 647, 761, 1040, 1418, 2644, 3187, 3332, 3482
ScrFI CCNGG 5 cut(s) 2565, 2903, 3634, 3648, 3681
SduI GDGCHC 5 cut(s) 180, 2029, 3078, 3640, 3665
SexAI ACCWGGT 2 cut(s) 2901, 3646
SfcI CTRYAG 5 cut(s) 576, 1914, 1985, 1996, 2447
Sfr274I CTCGAG 1 cut(s) 179
Sfr303I CCGCGG 1 cut(s) 2930
SfuI TTCGAA 3 cut(s) 47, 224, 1966
SgrBI CCGCGG 1 cut(s) 2930
SinI GGWCC 4 cut(s) 735, 1315, 3123, 3204
SlaI CTCGAG 1 cut(s) 179
SmiMI CAYNNNNRTG 5 cut(s) 162, 2135, 2198, 2237, 3314
SmlI CTYRAG 2 cut(s) 179, 2252
SmoI CTYRAG 2 cut(s) 179, 2252
SnaBI TACGTA 1 cut(s) 2179
SpeI ACTAGT 1 cut(s) 1352
SseBI AGGCCT 1 cut(s) 427
SsiI CCGC 8 cut(s) 608, 1778, 1971, 2104, 2847, 2927, 2929, 3052
SspMI CTAG 8 cut(s) 1353, 1500, 1623, 1800, 3009, 3222, 3407, 3473
StuI AGGCCT 1 cut(s) 427
StyD4I CCNGG 5 cut(s) 2563, 2901, 3632, 3646, 3679
StyI CCWWGG 5 cut(s) 255, 492, 2850, 3309, 3655
TaiI ACGT 6 cut(s) 1043, 1575, 2114, 2181, 3430, 3458
TaqI TCGA 7 cut(s) 47, 180, 224, 503, 1439, 1966, 2148
TaqII GACCGA 1 cut(s) 2108
TatI WGTACW 2 cut(s) 94, 2000
TauI GCSGC 1 cut(s) 2929
Tru1I TTAA 6 cut(s) 525, 1323, 1593, 1823, 1976, 2409
Tru9I TTAA 6 cut(s) 525, 1323, 1593, 1823, 1976, 2409
TscAI CASTG 9 cut(s) 150, 169, 252, 341, 863, 1162, 3154, 3175, 3214
TseFI GTSAC 7 cut(s) 689, 2608, 2684, 2888, 3160, 3190, 3650
TseI GCWGC 8 cut(s) 34, 433, 850, 1835, 1995, 3153, 3373, 3520
Tsp45I GTSAC 7 cut(s) 689, 2608, 2684, 2888, 3160, 3190, 3650
TspGWI ACGGA 1 cut(s) 1356
TspRI CASTG 9 cut(s) 150, 169, 252, 341, 863, 1162, 3154, 3175, 3214
Van91I CCANNNNNTGG 2 cut(s) 108, 3590
VpaK11BI GGWCC 4 cut(s) 735, 1315, 3123, 3204
XagI CCTNNNNNAGG 1 cut(s) 1138
XapI RAATTY 4 cut(s) 1325, 1513, 1729, 3709
XceI RCATGY 4 cut(s) 415, 1820, 3511, 3625
XcmI CCANNNNNNNNNTGG 3 cut(s) 2628, 2882, 3587
XhoI CTCGAG 1 cut(s) 179
XmnI GAANNNNTTC 1 cut(s) 2312
XspI CTAG 8 cut(s) 1353, 1500, 1623, 1800, 3009, 3222, 3407, 3473
ZrmI AGTACT 1 cut(s) 2002
Zsp2I ATGCAT 2 cut(s) 1897, 3102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.