Rw5G026450

metal ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000343
Physical Location & Seq
Forward (+)
38278112 .. 38290760
12649 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G026450.1

Sequence Viewer

Length: 3447 bp
ATGACCAATATGGTGAAACTTTCATGTTCACTAGTGGTCATCATGGAGAATCCCTTGGTTATGATTTTGATGATGGAGAAGTTGCAGCAAAGGGACATAAAGAGAGATCCTCTCATAGTGGTAACAAAGAGGGTGAAAGAAGTGTTTGAATTCAAGGAGGAGATGGAATCGTCAGATGATGAGGCTGAGGCAATGCAGCTTTCGGTTTCGAATTACTACTTTGTAGATGGCAAGGAAGATCCTATCTCCTTTCATGTACTGCCAATTCAATGGAGTGAGGGTGATAGGCAGGATGGGAAGGAAGCAGTGTTGTTTCTGCATGGCAGTGCCGACAATGGGCTCGAGAAGATTTACAAACCTGTTGTTGCTTGGAAGTTTGATCTTTCGAATGTGAAGCCCGAGATTGCAGTGCTTTCCAAGGAGAATGATTGGATTCTGCTCCAGAAGCCGAGGAGGAGCTATGAGGGGATCGTAAGGACTGTCTTGATAACTGTGCAGTGCCTTAGTTATGCAATGTGGAATGCTGAGGCGTCCGGAAAAGCTGTGTGGGATCACTTGTCTAAAGCGTTCAGCATGTATGAGAACAGGCCTTCGCAGCATGATTTGGGTGATCACAGGAGTCTGATTAGTGAAGCTCTAAAGCGAGATGATGCCTTGGCAAAGTCGAAGTTTATAATTGACTTTCTTAAAGAAGAGCCACCAACAAAGAGGAAACTATCTGATGAGGATATTCAAGCTACAGCAAAGCTTGGATTTATAGTTGATGATGCGGAAGATTATATAATTGATGATGAAGATGAGTCAGATGATGATGATGATGATAACCTGTTTGATTCTGTTTGTGCCTTTTGTGATAATGGTGGCAAGCTGTTATGTTGTGAAGGGAGATGCCTAAGGTCCTTCCATCCCACGGTAGAGGACGGCGAGGACTCAATGTGTGAATCTCTTGGATTCACTCGGGAGGAAGTAGATGCAATGGCAAGTTTCTTCTGCAAGAACTGTCAATATAAGCAGCATCAGTGCTTTGCTTGTGGGAAGTTAGGCTCCTCAGACAAATCTTTGGGTGCAGAGGTCTTCCCCTGTGTTTCTGCAACCTGTGGTCAATTTTACCATCCTCATTGTGTTGCACAACTAATTTATCAAGACAATGGAGTTTCTGCTGAAGAACTGGAGAAAAATATCTCTCTGGGGGAGTCTTTTACGTGTCCGATTCATAAGTGCTGTGTTTGTAAGCAAGGAGAGAATAAGAAGGATCCCGAGTTACGGTTTGCTGTGTGCAGGCGTTGTCCTAAATCCTATCACAGGAAATGCTTGCCCAGTGAGATTCGTTTTGAGAAAACAGAGGAAGATGATGAGGAAGAGATAGAGGATGAAGAAGAAATAGAAACAAGAGCTTGGGAAGGTCTATTACCTAATCGCATACTAATATATTGCACAGAACACGAAATAGATGAAGACATTGGAACTCCAATAAGGGACCATGTTAAATTTCCTGATTTGACGGAAAGAAGACTAAAAAAGAGAAGACTGACATCAGAGTCCCTTGTGGTCAGAGATAATTCTCTGTCGAAGAAAAGAAAGCTTTCCTCAGAGGGATTTTATGGTGGAAGAACTGCACCTCCAATATCTAAGCAGAAATTGAATTCGGGTGAAAAAGTTGGCAGTAGCAGAAATACTGAAAAAGTACCTTCTCGGTTAGACGTATCAAGAAAGGTGATGGTTAATAAGACATTGAAAAAGGAAGTTCAGACTAGCGTGGAAAAGAAGACCTCGTTGGGTAATAGACTGTTTACCTACATAAAGGGATCTGGGTCAGTAAAGTTTGGGAAGCAGGATACACCTGATGATACTGAACTAAATTCTAAAACGAGTGCAAAAATTGATCCTACATCAAGAACATTGAGTGCGGCAGCTTCCTTAGATCCTGCTAGAGAAAGGAGATTACATGCTTTAATGAAAGACGCTGCTTCATCAATAAGATTGGAAGATGTTATAGAAAAGCACAAAGTTCCATCCACTCATGCATCCTCAAATAAATATGCTGTAGAAAGGAACATAACACGAGGAAAGGTGGAAGGCTCAGTTGAGGCTGTTCGAACCGCATTAAAGAAACTACAGGAAGGCTGCAGTACTGAAGATGCAGAGGCTGTCTGTGCTCCTGAGATTCTTTCCCAGATTTATAAGTGGAAGAGCAAGCTTAGAGTATATCTTGCACCTTTCTTGCATGGAAAGCGGTACACGTCCTTCGGTCGCCATTTCACAAAGGTGGAAAAACTCGAACAGATTGCTGATAGGCTCCATTCGTACGTACAGAATGGAGACACGATAGTGGACTTCTGTTGTGGCTCTAATGATTTCAGCATCGTAATGAAGAAAAAGCTTGAGGAGACAGGGAAGAAGTGCTATTTTAGAAATTATGACATTATTCAACCAAAGAATGATTTCTGTTTTGAGAAGAGAGATTGGATGACTGTTCAAAAAGGTGAATTACCGGATAGTAGTAAATTGATCATGGGGTTGAACCCTCCTTTTGGGGTTAAAGCATCTTTGGCAAACCAGTTTATTGATAAGGCTCTGCAGTTCCAGCCGAAGCTCCTTATTCTTATTGTTCCACCAGAAACAAAAGGGTTACAATGTTATGATCTGATATGGAAGGATGAACGGTCTCTGTCGGGAAAGTCCTTTTATCTTCCTGGATCTGTTGATGAGAATGACAAGCAAATGGATCAATGGAATGTGACGACACCTCCACTCTATCTTTGGAGTCGTCCTGATTGGTCTGAGAGACACAGAGCTATTGCTCAAAAGGATAGTCACGGTTCCATGTCAGGGCCAGTGGAAGACGGTCATTCAGTTGAGAATAAGGATGAAAATGCTGAAAATCTCATGTTGAAAAATGCTTATCTGACACCGACCGGTAATTCCAGAGATTTTGTTGGTGGGGCAGTTGCAGGCGAGGGACACGAAGATAGATCCTCCAAGGGCAATGATGACAGAGGTTACCATGCGAGTTCTGGTCACCAGAAGAACCAGGTTAGTAAGATTTCTGAGAGGCCGCGGTGTGGTGGCAGAAAAAAGAATGGGAGTGGAGTGGTTGAGTTGTCACCAGACAACAAATGTGATGGTGGCAATGTTTCTAGTGAGATTCAGATGGAAAGGTCTCCATCTACTGAGCGACAGCGGAAAGCAAATCAGCAGTCTGAGCCCAGCGATTCTGGTTCAAGTATGCATTTCAGAACTGCTTGTGGGAGGACCACAGCCAGCATCCCTGATGACACTGGCAGCAATGTCACAAGCAGTGCTGACCCTTATTCAAGTGTGACTCATAGATGGTCCAGTGGTGCCAGTCCTAGCTCTATATATAGGGATACAAAATTGGAGGAACCTGGTTCTCACCAATGGGGATGCTTCTCAATACCGGAATTCAGCAGGTCAGGATTTGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006325 GO:0006355 GO:0006950 GO:0006952 GO:0006996 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0009605 GO:0009607 GO:0009620 GO:0009791 GO:0009889 GO:0009893 GO:0009909 GO:0009911 GO:0009987 GO:0010228 GO:0010468 GO:0010556 GO:0010604 GO:0010638 GO:0016043 GO:0019219 GO:0019222 GO:0022414 GO:0023052 GO:0030154 GO:0031056 GO:0031058 GO:0031060 GO:0031062 GO:0031323 GO:0031325 GO:0031326 GO:0031399 GO:0031401 GO:0031440 GO:0031935 GO:0031937 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033043 GO:0033044 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0043954 GO:0044030 GO:0044424 GO:0044464 GO:0048366 GO:0048367 GO:0048468 GO:0048518 GO:0048522 GO:0048580 GO:0048582 GO:0048608 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0048869 GO:0050684 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051052 GO:0051094 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051276 GO:0051570 GO:0051574 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060968 GO:0061085 GO:0061087 GO:0061458 GO:0065007 GO:0070827 GO:0070828 GO:0070829 GO:0071840 GO:0080090 GO:0090436 GO:0097159 GO:0098542 GO:0099402 GO:0140110 GO:1900109 GO:1900111 GO:1900363 GO:1901363 GO:1902275 GO:1903311 GO:1903506 GO:1905269 GO:2000024 GO:2000026 GO:2000112 GO:2000241 GO:2000243 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNMT1-RFD

Protein Analysis

1148

Amino Acids

129.23

Weight (kDa)

5.89

Isoelectric Point (pI)

45.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNMT1-RFD PF12047 67 - 165 1.7e-06 Cytosine specific DNA methyltransferase replication foci domain
PHD_NSD PF22908 337 - 405 1.3e-19 Histone-lysine N-methyltransferase NSD-like, PHD zinc finger
Mtase_EDM2 PF26055 769 - 935 3.7e-88 EDM2 methyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000343)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48090 AT5G48090 AT5G48090 AT5G55390 AT5G55390 AT5G55390
fragaria_vesca FvH4_3g23690 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160
malus_domestica MD03G1191000.v1.1 MD11G1207800.v1.1 MD11G1208000.v1.1
prunus_persica Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227600_v2.0.a1 Prupe.4G227700_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1
pyrus_communis pycom01g03400 pycom03g14420 pycom11g18100 pycom11g18140 pycom11g18150 pycom11g18160
rosa_chinensis RchiOBHm_Chr2g0145981 RchiOBHm_Chr4g0405241 RchiOBHm_Chr4g0405271 RchiOBHm_Chr5g0016201 RchiOBHm_Chr5g0041831 RchiOBHm_Chr5g0041871 RchiOBHm_Chr5g0041881 RchiOBHm_Chr5g0059241
rosa_laevigata RLG00000002117 RLG00000008861 RLG00000008862 RLG00000011000 RLG00000033216 RLG00000034097 RLG00000034101
rosa_multiflora Rmu_sc0000161.1_g000001 Rmu_sc0000861.1_g000040 Rmu_sc0007522.1_g000003 Rmu_sc0009443.1_g000015 Rmu_sc0022129.1_g000001 Rmu_sc0042315.1_g000001
rosa_roxburghii Rroxscaffold_1G00038660 Rroxscaffold_1G00038690 Rroxscaffold_5G00349560 Rroxscaffold_5G00349610 Rroxscaffold_6G00388370 Rroxscaffold_7G00188740
rosa_rugosa Rorug03G0199700 Rorug04G0055400 Rorug04G0055500 Rorug04G0055900 Rorug04G0056000 Rorug04G0056100 Rorug04G0056100 Rorug04G0056200 Rorug05G0195600 Rorug05G0195700 Rorug05G0195800 Rorug05G0195900 Rorug05G0196800 Rorug05G0385000
rosa_samantha Rh4AG129900 Rh4AG130100 Rh4BG124200 Rh4BG124400 Rh4BG124500 Rh4CG137200 Rh4CG137300 Rh4DG123500 Rh4DG123700 Rh5AG280800 Rh5AG281400 Rh5BG286700 Rh5BG287000 Rh5BG397800 Rh5CG318100 Rh5CG318600 Rh5CG421600 Rh5DG132300 Rh5DG132400 Rh5DG295400 Rh5DG295700 Rh7BG475300
rosa_wichuraiana Rw0G001330 Rw0G005730 Rw4G008670 Rw4G010510 Rw4G010540 Rw5G026450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 674, 2181
AasI GACNNNNNNGTC 1 cut(s) 1537
Acc36I ACCTGC 1 cut(s) 3420
AccB1I GGYRCC 1 cut(s) 3341
AccII CGCG 1 cut(s) 3058
AccIII TCCGGA 1 cut(s) 533
AciI CCGC 7 cut(s) 770, 1907, 2100, 2233, 3056, 3058, 3181
AcsI RAATTY 5 cut(s) 149, 1487, 1642, 1858, 3422
AcuI CTGAAG 2 cut(s) 1182, 2154
AcyI GRCGYC 1 cut(s) 530
AfaI GTAC 6 cut(s) 258, 1686, 2131, 2237, 2306, 2310
AfiI CCNNNNNNNGG 7 cut(s) 336, 910, 1263, 1302, 2531, 2829, 3062
AflIII ACRYGT 2 cut(s) 1202, 2238
AgeI ACCGGT 1 cut(s) 2915
AhlI ACTAGT 1 cut(s) 31
AjiI CACGTC 1 cut(s) 2241
AjnI CCWGG 3 cut(s) 2692, 3030, 3385
AjuI GAANNNNNNNTTGG 2 cut(s) 1757, 1789
AleI CACNNNNGTG 2 cut(s) 2264, 2327
AloI GAACNNNNNNTCC 2 cut(s) 1603, 1635
Alw21I GWGCWC 1 cut(s) 2158
Alw26I GTCTC 5 cut(s) 2313, 2381, 2670, 2779, 3165
AlwNI CAGNNNCTG 1 cut(s) 2147
Ama87I CYCGRG 4 cut(s) 341, 398, 957, 1256
Aor13HI TCCGGA 1 cut(s) 533
AoxI GGCC 3 cut(s) 587, 2831, 3053
ApeKI GCWGC 8 cut(s) 85, 196, 595, 1012, 1910, 1964, 2124, 3282
ApoI RAATTY 5 cut(s) 149, 1487, 1642, 1858, 3422
ArsI GACNNNNNNTTYG 4 cut(s) 2797, 2829, 3182, 3214
AsiGI ACCGGT 1 cut(s) 2915
Asp700I GAANNNNTTC 2 cut(s) 1582, 2441
AspS9I GGNCC 5 cut(s) 897, 1477, 2831, 3252, 3333
AsuII TTCGAA 3 cut(s) 209, 386, 2095
AvaI CYCGRG 4 cut(s) 341, 398, 957, 1256
AvaII GGWCC 4 cut(s) 897, 1477, 3252, 3333
AxyI CCTNAGG 1 cut(s) 893
BamHI GGATCC 1 cut(s) 1252
BanI GGYRCC 1 cut(s) 3341
BanII GRGCYC 2 cut(s) 342, 3207
BarI GAAGNNNNNNTAC 4 cut(s) 1392, 1424, 2228, 2260
BauI CACGAG 1 cut(s) 2061
BbsI GAAGAC 6 cut(s) 1066, 1461, 1516, 1531, 1772, 2847
Bbv12I GWGCWC 1 cut(s) 2158
BbvCI CCTCAGC 2 cut(s) 186, 525
BbvI GCAGC 8 cut(s) 97, 208, 607, 1024, 1922, 1951, 2111, 3294
BceAI ACGGC 1 cut(s) 937
BcgI CGANNNNNNTGC 2 cut(s) 2267, 2301
BciT130I CCWGG 3 cut(s) 2694, 3032, 3387
BciVI GTATCC 2 cut(s) 1828, 3361
BclI TGATCA 2 cut(s) 610, 2508
BcoDI GTCTC 5 cut(s) 2313, 2381, 2670, 2779, 3165
BcuI ACTAGT 1 cut(s) 31
BfaI CTAG 5 cut(s) 32, 1752, 1929, 3138, 3351
BfmI CTRYAG 5 cut(s) 738, 2043, 2114, 2125, 2576
BfuAI ACCTGC 1 cut(s) 3420
BfuI GTATCC 2 cut(s) 1828, 3361
BmcAI AGTACT 1 cut(s) 2131
Bme1390I CCNGG 3 cut(s) 2694, 3032, 3387
Bme18I GGWCC 4 cut(s) 897, 1477, 3252, 3333
BmeT110I CYCGRG 4 cut(s) 341, 398, 957, 1256
BmgBI CACGTC 1 cut(s) 2241
BmgT120I GGNCC 5 cut(s) 897, 1477, 2831, 3252, 3333
BmiI GGNNCC 7 cut(s) 1045, 1254, 1478, 2297, 2821, 3343, 3384
BmrFI CCNGG 3 cut(s) 2694, 3032, 3387
BmrI ACTGGG 1 cut(s) 1311
BmuI ACTGGG 1 cut(s) 1311
BpiI GAAGAC 6 cut(s) 1066, 1461, 1516, 1531, 1772, 2847
BplI GAGNNNNNCTC 4 cut(s) 94, 126, 96, 128
BpmI CTGGAG 2 cut(s) 425, 1190
Bpu10I CCTNAGC 2 cut(s) 186, 525
Bpu14I TTCGAA 3 cut(s) 209, 386, 2095
BpuEI CTTGAG 1 cut(s) 2402
BsaAI YACGTR 2 cut(s) 1203, 2308
BsaHI GRCGYC 1 cut(s) 530
BsaI GGTCTC 2 cut(s) 2670, 3165
BsaJI CCNNGG 7 cut(s) 54, 417, 449, 654, 909, 2979, 3056
BsaWI WCCGGW 4 cut(s) 533, 2491, 2915, 3418
BsaXI ACNNNNNCTCC 6 cut(s) 265, 295, 1603, 1633, 2731, 2761
Bsc4I CCNNNNNNNGG 7 cut(s) 336, 910, 1263, 1302, 2531, 2829, 3062
Bse118I RCCGGY 1 cut(s) 2915
Bse1I ACTGG 7 cut(s) 1173, 1317, 2557, 2834, 3283, 3336, 3345
Bse21I CCTNAGG 1 cut(s) 893
Bse3DI GCAATG 6 cut(s) 198, 519, 981, 2992, 3136, 3292
BseAI TCCGGA 1 cut(s) 533
BseBI CCWGG 3 cut(s) 2694, 3032, 3387
BseDI CCNNGG 7 cut(s) 54, 417, 449, 654, 909, 2979, 3056
BseLI CCNNNNNNNGG 7 cut(s) 336, 910, 1263, 1302, 2531, 2829, 3062
BseMI GCAATG 6 cut(s) 198, 519, 981, 2992, 3136, 3292
BseNI ACTGG 7 cut(s) 1173, 1317, 2557, 2834, 3283, 3336, 3345
BseRI GAGGAG 5 cut(s) 173, 466, 469, 1036, 2399
BseXI GCAGC 8 cut(s) 97, 208, 607, 1024, 1922, 1951, 2111, 3294
BseYI CCCAGC 1 cut(s) 3206
BsgI GTGCAG 4 cut(s) 515, 1086, 1297, 1599
Bsh1236I CGCG 1 cut(s) 3058
Bsh1285I CGRYCG 2 cut(s) 2251, 2916
BshFI GGCC 3 cut(s) 589, 2833, 3055
BshNI GGYRCC 1 cut(s) 3341
BshTI ACCGGT 1 cut(s) 2915
BsiEI CGRYCG 2 cut(s) 2251, 2916
BsiHKAI GWGCWC 1 cut(s) 2158
BsiHKCI CYCGRG 4 cut(s) 341, 398, 957, 1256
BsiSI CCGG 4 cut(s) 534, 2492, 2916, 3419
BsiWI CGTACG 1 cut(s) 2304
BslFI GGGAC 4 cut(s) 107, 1490, 1525, 2973
BslI CCNNNNNNNGG 7 cut(s) 336, 910, 1263, 1302, 2531, 2829, 3062
BsmAI GTCTC 5 cut(s) 2313, 2381, 2670, 2779, 3165
BsmFI GGGAC 4 cut(s) 107, 1490, 1525, 2973
BsmI GAATGC 1 cut(s) 526
BsnI GGCC 3 cut(s) 589, 2833, 3055
Bso31I GGTCTC 2 cut(s) 2670, 3165
BsoBI CYCGRG 4 cut(s) 341, 398, 957, 1256
Bsp119I TTCGAA 3 cut(s) 209, 386, 2095
Bsp1286I GDGCHC 3 cut(s) 342, 2158, 3207
Bsp13I TCCGGA 1 cut(s) 533
BspACI CCGC 7 cut(s) 770, 1907, 2100, 2233, 3056, 3058, 3181
BspANI GGCC 3 cut(s) 589, 2833, 3055
BspEI TCCGGA 1 cut(s) 533
BspFNI CGCG 1 cut(s) 3058
BspLI GGNNCC 7 cut(s) 1045, 1254, 1478, 2297, 2821, 3343, 3384
BspMAI CTGCAG 2 cut(s) 2129, 2580
BspMI ACCTGC 1 cut(s) 3420
BspQI GCTCTTC 2 cut(s) 687, 2183
BspT104I TTCGAA 3 cut(s) 209, 386, 2095
BspT107I GGYRCC 1 cut(s) 3341
BspTNI GGTCTC 2 cut(s) 2670, 3165
BsrDI GCAATG 6 cut(s) 198, 519, 981, 2992, 3136, 3292
BsrFI RCCGGY 1 cut(s) 2915
BsrI ACTGG 7 cut(s) 1173, 1317, 2557, 2834, 3283, 3336, 3345
BssAI RCCGGY 1 cut(s) 2915
BssECI CCNNGG 7 cut(s) 54, 417, 449, 654, 909, 2979, 3056
BssNI GRCGYC 1 cut(s) 530
BssSI CACGAG 1 cut(s) 2061
BssT1I CCWWGG 4 cut(s) 54, 417, 654, 2979
Bst2BI CACGAG 1 cut(s) 2061
Bst2UI CCWGG 3 cut(s) 2694, 3032, 3387
Bst6I CTCTTC 4 cut(s) 687, 1353, 2183, 2450
BstACI GRCGYC 1 cut(s) 530
BstBAI YACGTR 2 cut(s) 1203, 2308
BstBI TTCGAA 3 cut(s) 209, 386, 2095
BstC8I GCNNGC 6 cut(s) 866, 1280, 1313, 2195, 2953, 3262
BstDSI CCRYGG 2 cut(s) 909, 3056
BstEII GGTNACC 2 cut(s) 2999, 3017
BstENI CCTNNNNNAGG 1 cut(s) 1300
BstFNI CGCG 1 cut(s) 3058
BstMAI GTCTC 5 cut(s) 2313, 2381, 2670, 2779, 3165
BstMCI CGRYCG 2 cut(s) 2251, 2916
BstMWI GCNNNNNNNGC 7 cut(s) 445, 595, 2153, 2230, 2549, 2584, 3202
BstNI CCWGG 3 cut(s) 2694, 3032, 3387
BstNSI RCATGY 2 cut(s) 577, 1949
BstPI GGTNACC 2 cut(s) 2999, 3017
BstSCI CCNGG 3 cut(s) 2692, 3030, 3385
BstSFI CTRYAG 5 cut(s) 738, 2043, 2114, 2125, 2576
BstSNI TACGTA 1 cut(s) 2308
BstUI CGCG 1 cut(s) 3058
BstV1I GCAGC 8 cut(s) 97, 208, 607, 1024, 1922, 1951, 2111, 3294
BstV2I GAAGAC 6 cut(s) 1066, 1461, 1516, 1531, 1772, 2847
BstX2I RGATCY 7 cut(s) 106, 238, 1252, 1805, 1921, 2696, 2972
BstXI CCANNNNNNTGG 1 cut(s) 270
BstYI RGATCY 7 cut(s) 106, 238, 1252, 1805, 1921, 2696, 2972
Bsu36I CCTNAGG 1 cut(s) 893
BsuI GTATCC 2 cut(s) 1828, 3361
BsuRI GGCC 3 cut(s) 589, 2833, 3055
BtgI CCRYGG 2 cut(s) 909, 3056
BtrI CACGTC 1 cut(s) 2241
BtsI GCAGTG 5 cut(s) 312, 331, 414, 503, 3304
BveI ACCTGC 1 cut(s) 3420
Cac8I GCNNGC 6 cut(s) 866, 1280, 1313, 2195, 2953, 3262
CaiI CAGNNNCTG 1 cut(s) 2147
Cfr10I RCCGGY 1 cut(s) 2915
Cfr13I GGNCC 5 cut(s) 897, 1477, 2831, 3252, 3333
Cfr42I CCGCGG 1 cut(s) 3059
CseI GACGC 2 cut(s) 519, 1970
CsiI ACCWGGT 2 cut(s) 3030, 3385
Csp6I GTAC 6 cut(s) 257, 1685, 2130, 2236, 2305, 2309
CspAI ACCGGT 1 cut(s) 2915
CviQI GTAC 6 cut(s) 257, 1685, 2130, 2236, 2305, 2309
DrdI GACNNNNNNGTC 1 cut(s) 1537
DseDI GACNNNNNNGTC 1 cut(s) 1537
Eam1104I CTCTTC 4 cut(s) 687, 1353, 2183, 2450
EarI CTCTTC 4 cut(s) 687, 1353, 2183, 2450
Eco105I TACGTA 1 cut(s) 2308
Eco130I CCWWGG 4 cut(s) 54, 417, 654, 2979
Eco147I AGGCCT 1 cut(s) 589
Eco24I GRGCYC 2 cut(s) 342, 3207
Eco31I GGTCTC 2 cut(s) 2670, 3165
Eco47I GGWCC 4 cut(s) 897, 1477, 3252, 3333
Eco57I CTGAAG 2 cut(s) 1182, 2154
Eco81I CCTNAGG 1 cut(s) 893
Eco88I CYCGRG 4 cut(s) 341, 398, 957, 1256
Eco91I GGTNACC 2 cut(s) 2999, 3017
EcoNI CCTNNNNNAGG 1 cut(s) 1300
EcoO109I RGGNCCY 1 cut(s) 897
EcoO65I GGTNACC 2 cut(s) 2999, 3017
EcoRI GAATTC 3 cut(s) 149, 1642, 3422
EcoRII CCWGG 3 cut(s) 2692, 3030, 3385
EcoT14I CCWWGG 4 cut(s) 54, 417, 654, 2979
EcoT22I ATGCAT 2 cut(s) 2026, 3231
EcoT38I GRGCYC 2 cut(s) 342, 3207
ErhI CCWWGG 4 cut(s) 54, 417, 654, 2979
FaqI GGGAC 4 cut(s) 107, 1490, 1525, 2973
FbaI TGATCA 2 cut(s) 610, 2508
FriOI GRGCYC 2 cut(s) 342, 3207
FspBI CTAG 5 cut(s) 32, 1752, 1929, 3138, 3351
GsaI CCCAGC 1 cut(s) 3210
GsuI CTGGAG 2 cut(s) 425, 1190
HaeIII GGCC 3 cut(s) 589, 2833, 3055
HapII CCGG 4 cut(s) 534, 2492, 2916, 3419
HgaI GACGC 2 cut(s) 519, 1970
Hin1I GRCGYC 1 cut(s) 530
HindIII AAGCTT 4 cut(s) 746, 1580, 2195, 2378
HpaII CCGG 4 cut(s) 534, 2492, 2916, 3419
Hpy166II GTNNAC 4 cut(s) 29, 1791, 2238, 2332
Hpy8I GTNNAC 4 cut(s) 29, 1791, 2238, 2332
HpyCH4IV ACGT 4 cut(s) 1202, 1701, 2240, 2307
HpyF10VI GCNNNNNNNGC 7 cut(s) 445, 595, 2153, 2230, 2549, 2584, 3202
HpySE526I ACGT 4 cut(s) 1202, 1701, 2240, 2307
Hsp92I GRCGYC 1 cut(s) 530
Kpn2I TCCGGA 1 cut(s) 533
Ksp22I TGATCA 2 cut(s) 610, 2508
KspI CCGCGG 1 cut(s) 3059
LguI GCTCTTC 2 cut(s) 687, 2183
LmnI GCTCC 6 cut(s) 444, 456, 1049, 2161, 2301, 2598
Lsp1109I GCAGC 8 cut(s) 97, 208, 607, 1024, 1922, 1951, 2111, 3294
MabI ACCWGGT 2 cut(s) 3030, 3385
MaeI CTAG 5 cut(s) 32, 1752, 1929, 3138, 3351
MaeII ACGT 4 cut(s) 1202, 1701, 2240, 2307
MflI RGATCY 7 cut(s) 106, 238, 1252, 1805, 1921, 2696, 2972
MhlI GDGCHC 3 cut(s) 342, 2158, 3207
MlyI GAGTC 7 cut(s) 628, 809, 923, 1202, 1547, 2773, 3316
Mph1103I ATGCAT 2 cut(s) 2026, 3231
MroI TCCGGA 1 cut(s) 533
MroXI GAANNNNTTC 2 cut(s) 1582, 2441
MseI TTAA 6 cut(s) 687, 1485, 1722, 1952, 2105, 2538
MslI CAYNNNNRTG 4 cut(s) 324, 2264, 2327, 2366
MspA1I CMGCKG 2 cut(s) 3058, 3181
MspI CCGG 4 cut(s) 534, 2492, 2916, 3419
MspR9I CCNGG 3 cut(s) 2694, 3032, 3387
Mva1269I GAATGC 1 cut(s) 526
MvaI CCWGG 3 cut(s) 2694, 3032, 3387
MvnI CGCG 1 cut(s) 3058
MwoI GCNNNNNNNGC 7 cut(s) 445, 595, 2153, 2230, 2549, 2584, 3202
NlaIV GGNNCC 7 cut(s) 1045, 1254, 1478, 2297, 2821, 3343, 3384
NmeAIII GCCGAG 1 cut(s) 474
NmuCI GTSAC 6 cut(s) 2737, 2813, 3017, 3102, 3289, 3319
NsiI ATGCAT 2 cut(s) 2026, 3231
NspI RCATGY 2 cut(s) 577, 1949
NspV TTCGAA 3 cut(s) 209, 386, 2095
OliI CACNNNNGTG 2 cut(s) 2264, 2327
PaeR7I CTCGAG 1 cut(s) 341
PceI AGGCCT 1 cut(s) 589
PciSI GCTCTTC 2 cut(s) 687, 2183
PctI GAATGC 1 cut(s) 526
PdmI GAANNNNTTC 2 cut(s) 1582, 2441
Pfl23II CGTACG 1 cut(s) 2304
PfoI TCCNGGA 1 cut(s) 2692
PinAI ACCGGT 1 cut(s) 2915
PleI GAGTC 7 cut(s) 627, 808, 923, 1201, 1546, 2772, 3316
PpsI GAGTC 7 cut(s) 627, 808, 923, 1201, 1546, 2772, 3316
Ppu21I YACGTR 2 cut(s) 1203, 2308
PpuMI RGGWCCY 1 cut(s) 897
PsiI TTATAA 2 cut(s) 674, 2181
Psp5II RGGWCCY 1 cut(s) 897
Psp6I CCWGG 3 cut(s) 2692, 3030, 3385
PspEI GGTNACC 2 cut(s) 2999, 3017
PspFI CCCAGC 1 cut(s) 3206
PspGI CCWGG 3 cut(s) 2692, 3030, 3385
PspLI CGTACG 1 cut(s) 2304
PspN4I GGNNCC 7 cut(s) 1045, 1254, 1478, 2297, 2821, 3343, 3384
PspPI GGNCC 5 cut(s) 897, 1477, 2831, 3252, 3333
PspPPI RGGWCCY 1 cut(s) 897
PstI CTGCAG 2 cut(s) 2129, 2580
PstNI CAGNNNCTG 1 cut(s) 2147
PsuI RGATCY 7 cut(s) 106, 238, 1252, 1805, 1921, 2696, 2972
RsaI GTAC 6 cut(s) 258, 1686, 2131, 2237, 2306, 2310
RsaNI GTAC 6 cut(s) 257, 1685, 2130, 2236, 2305, 2309
RseI CAYNNNNRTG 4 cut(s) 324, 2264, 2327, 2366
SacII CCGCGG 1 cut(s) 3059
SapI GCTCTTC 2 cut(s) 687, 2183
SaqAI TTAA 6 cut(s) 687, 1485, 1722, 1952, 2105, 2538
Sau96I GGNCC 5 cut(s) 897, 1477, 2831, 3252, 3333
ScaI AGTACT 1 cut(s) 2131
SchI GAGTC 7 cut(s) 628, 809, 923, 1202, 1547, 2773, 3316
ScrFI CCNGG 3 cut(s) 2694, 3032, 3387
SduI GDGCHC 3 cut(s) 342, 2158, 3207
SexAI ACCWGGT 2 cut(s) 3030, 3385
SfcI CTRYAG 5 cut(s) 738, 2043, 2114, 2125, 2576
Sfr274I CTCGAG 1 cut(s) 341
Sfr303I CCGCGG 1 cut(s) 3059
SfuI TTCGAA 3 cut(s) 209, 386, 2095
SgrBI CCGCGG 1 cut(s) 3059
SinI GGWCC 4 cut(s) 897, 1477, 3252, 3333
SlaI CTCGAG 1 cut(s) 341
SmiMI CAYNNNNRTG 4 cut(s) 324, 2264, 2327, 2366
SmlI CTYRAG 2 cut(s) 341, 2381
SmoI CTYRAG 2 cut(s) 341, 2381
SnaBI TACGTA 1 cut(s) 2308
SpeI ACTAGT 1 cut(s) 31
SseBI AGGCCT 1 cut(s) 589
SsiI CCGC 7 cut(s) 770, 1907, 2100, 2233, 3056, 3058, 3181
SspMI CTAG 5 cut(s) 32, 1752, 1929, 3138, 3351
StuI AGGCCT 1 cut(s) 589
StyD4I CCNGG 3 cut(s) 2692, 3030, 3385
StyI CCWWGG 4 cut(s) 54, 417, 654, 2979
TaiI ACGT 4 cut(s) 1205, 1704, 2243, 2310
TaqI TCGA 7 cut(s) 209, 342, 386, 665, 1568, 2095, 2277
TaqII GACCGA 1 cut(s) 2237
TatI WGTACW 2 cut(s) 256, 2129
TauI GCSGC 2 cut(s) 1910, 3058
Tru1I TTAA 6 cut(s) 687, 1485, 1722, 1952, 2105, 2538
Tru9I TTAA 6 cut(s) 687, 1485, 1722, 1952, 2105, 2538
TseFI GTSAC 6 cut(s) 2737, 2813, 3017, 3102, 3289, 3319
TseI GCWGC 8 cut(s) 85, 196, 595, 1012, 1910, 1964, 2124, 3282
Tsp45I GTSAC 6 cut(s) 2737, 2813, 3017, 3102, 3289, 3319
TspGWI ACGGA 1 cut(s) 1517
VpaK11BI GGWCC 4 cut(s) 897, 1477, 3252, 3333
XagI CCTNNNNNAGG 1 cut(s) 1300
XapI RAATTY 5 cut(s) 149, 1487, 1642, 1858, 3422
XceI RCATGY 2 cut(s) 577, 1949
XcmI CCANNNNNNNNNTGG 2 cut(s) 2757, 3011
XhoI CTCGAG 1 cut(s) 341
XmnI GAANNNNTTC 2 cut(s) 1582, 2441
XspI CTAG 5 cut(s) 32, 1752, 1929, 3138, 3351
ZrmI AGTACT 1 cut(s) 2131
Zsp2I ATGCAT 2 cut(s) 2026, 3231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.