MD11G1208000.v1.1

metal ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0000343
Physical Location & Seq
Forward (+)
30292145 .. 30306612
14468 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1208000.v1.1.491

Sequence Viewer

Length: 3534 bp
ATGCAGATATTTGTGCGTGGCACTGCCGATAATGGGCTTCAGAAACTGTATAAGCATGTTATCGCATGGCGGTTTGATCTTTCCAATGTCGATCCTGAGATATCGTTGCTCTCTAAGGAAAATAACTGGCTCAACCTTCAGAAGCCGAGGAAGAGCTTCGAGGAAGTAATAAGGTCAATCTTGATAACAGTGCAGTTCCTTCACTATGTGAGGAGAAATCCTGAGACATCCGGCAAATCTTTGTGGGATCACTTATCTAAAGTTTTCAGCTTGTACGAGGTCAAGCCTTCTGAGAATGACCTGGTGAATCACATTCCTCTAATTAGTGAAGCTCTTAAACGGGATGATGCGTTAGCAAAGTGCAAGTTTTTAGTCAATTTACTTGAAGAGAAGACCAACAAGAGGAAACTGTATGATCAGGACATTCAAGCTACAACAAAACCTGGATTTATAGTTGATGATACGGAAGAAGATATGATTGACGCTGAAGACGAGTCCAACGACGATGATAACCTGTTTGATTCTGTTTGTGCTTTATGTGATGATGGTGGCGATCTTTTGCGTTGTGAAGGGAGATGCCTAAGGTCATTTCACGCAACTGTCAAGGATGGTGAGCATTCTATGTGTGAGACCCTTGGCTTTACCCAGGATGAAGTAGAAGCAATTCAAAACTTCTTCTGCAAGAACTGTCAACATAAACAACATCAATGCTATGCTTGTGGGAAGTTAGGCTCCTCTGATAAATCTTCAGGGGCTGAGGTCTTCCCCTGTGTTTCTGCAACCTGTGGTCAATTTTACCATCCACATTGCATTGCAAAACTCATTTATAAAGACAATGGAGTCTCTGCTGAAGAACTTGAGAAAAAAATTGCTCTGGGGGAATCTTTTACGTGTCCGATTCATAAATGCTGCATTTGTGAACAAGGAGAGAACAAGAAGGATCCTCAGTTGCAGTTTGCTGTGTGTCGGCGTTGTCCTAAATCCTACCACAGGAAATGCTTGCCAGAGGAGATTGTATTTGAAAAAACAAAGGAAGATGAAGAGGATGAAGACGAAGAGGAGGAAGACACAGAGGATGAAGGTACAACAACAAGAGCTTGGGAACATCTATTACCTAACCGTGTACTAATATATTGCACAAAACATGAGATGATTAAAGACATTGAAACGCCAATAAGGGACCACATAAAATTCCCTAGAGTTAAAGAAAAGAGGACTACCTTTATTCGAAAGAAGACCGATTTTGTAGAGAAAAAGAAAAAAGGGACATCAGAATCCCTTCAAGATAGAGAGATATCTGTGGCGAATAAGAGAAAGCTTTCCACCAAAGAATTCTCTAGGGGTCAAACTGCTCCTATGATATCTAAAGAGAAGCTGAAGTCATCTTCTACCGCAAAAGTGGGTGGTAGCAGAATTAGTAAAAAACTACCTGCTGGGTTAGATACATCAAGAAAGCTGAAAGTGAATTGTGCTTTGAAAAAGAAAGCTAAGATTTCTGTGGCTGGGGAACAAAATACCTCATTGGGTGATCAGCTATATGCCTATAGGAAGGAGTCTTTGCAAATGAAGTCGGGGAAGCAGGGCAAACCTGATGGGGAGCGTGATTTGGCAATATTTAATCCTGCCTCAAAAAAGTTGACCAGCGCACCACCATCTTTAGATGCAGCTACAGAGAGGAGGCTATTGTCTTTAATGAAAGATGCTGCGTCTTCAATAACTCTGGAAGATGTTATAAGAAAGCACACAGTTCCATCGACTCATCAATCCTCATCCAAAAATGCTGTGGAAAGGAGCATTACAATGGGAAAGGTAGAAGGCTCAATTGAGGCTGTTCGAACGGCGTTACGGAAACTAGAGGAAGGATGCAGTGCTGAAGATTCAGAGGCTGTTTGTGCTCCTGAGGTTGTTCACCAGATTTACAAGTGGAAGAACAAGCTGAGAGTATATCTTGCACCTTTCATGCATGGAATGCGCTACACGTCCTTTGGTCGTCATTTTACAAAGGTGGACAAATTAAAAGAGATTGCTGATAGGCTCCATTGGTATGCAAAAGATGGAGATACGATAGTGGACTTCTGTTGTGGTGCCAATGATTTCAGCATTGTAATGAAGAAGAAACTTGAAGAGACGGGGAAGAATTGCTTTTATAAAAACTATGACTGTATTCAACCGAAGAATGATTTCTGTTTTGAGAAGAGAGATTGGATGAAAGTAAAACCAAAGGAGTTACCGAAAGGGTCACAATTGATCATGGGGTTAAACCCTCCTTTCGGAGTTAAAGCAGCTTTGGCAAACAAGTTCATTGACAAGGCTCTTGAGTTCAATCCAAAGCTCCTTATTCTTATTGTTCCACCAGAAACTCAAGGGTTAAATGAAAAGAAATCGCCTTATGATTTGATCTGGGAGGATACCCAGTTTCTGTCAGGAAAGTCTTTTTATCTCCCCGGATCTGTTGATGCGAATGACAAACAATTGGATCAATGGAATGTAACGCCGCCACCACTTTATCTCTGGAGTCGTCCTGATTGGTCCGCTGACATCAAGGCTATAGCCCAAGAGCATGGTCACATCACTGCATCAGGGTATACGAAAGATCATTCTGACTCTTTAAATAACAGTCGGTCAATAGGTAATAATGATCAATATGGTGAAGCGCCTATGCTGATTGATGATGGCATAAAACCCGAGAGTCCTGGGGATGGGAACAACATTGATGAGATATGCAAGGAAATTCTGCCACGCATTCAGCCTGCTGAAAAAGGAGATCAGCATTCTGAGCCTGGCAACTCTGGTTCAAGCATGCAGTTCGGAACTACTTATGGTGGGACAAAGTTCAACATTGCTGATGACACAGGTAGAAGGAGCTTCTCCATGAGCAATGATGAGCCTTACTCGAGTCTGACTCATAGATGGTCTACCGGTCCCAATTCTGGTTATAGGGCTACAAACTTGGAGGAATTGTTTGTGGGGCACACGAGAGAGAGATTGGATTGCCTTGGTTGTACACAGGCAGAAGATGCATTTAGGAGGGAGTCTGACGTATGCTCTCAGATTCGCCTTTATGGTCAGCAAGATTTTGATTCTTCAAGAAGTAATTATTTGGTGGGTCAGGATTCTGTGAGTGGACAGATTGGATCGTATTCATCTACTGACAGCCATAGTCATCCTGGTCCTGCAGCTGAGTTATCGTACAGAATGAACACATCAGTCACGCAGCGGTATGCACCTCGGTTGGATGAATTGAACCACACGAGGATGGGCCGCTTAGGATCTGAACCTGCTATAGGATATCAACCGCACATGTCCAGTAGCAACGGCACTTATGATCCCAGGGCACCCCGGCCCGGTCAACATGGTGGCTCCATGGGTTTTGCTCCCGGTCCTCACCAAAGCTATTCGAACCAAAATTCAGCAGGAAGGATCAATCTGAATCAAAATTTAGACTTCAAATTTAACGGTTTACCGTCTCCTTTTAGTTCTCCCATCTCTCTGGATCTGCTGATGCGAATGGGAAACAAATGGATGAATGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006325 GO:0006355 GO:0006950 GO:0006952 GO:0006996 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0009605 GO:0009607 GO:0009620 GO:0009791 GO:0009889 GO:0009893 GO:0009909 GO:0009911 GO:0009987 GO:0010228 GO:0010468 GO:0010556 GO:0010604 GO:0010638 GO:0016043 GO:0019219 GO:0019222 GO:0022414 GO:0023052 GO:0030154 GO:0031056 GO:0031058 GO:0031060 GO:0031062 GO:0031323 GO:0031325 GO:0031326 GO:0031399 GO:0031401 GO:0031440 GO:0031935 GO:0031937 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033043 GO:0033044 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0043954 GO:0044030 GO:0044424 GO:0044464 GO:0048366 GO:0048367 GO:0048468 GO:0048518 GO:0048522 GO:0048580 GO:0048582 GO:0048608 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0048869 GO:0050684 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051052 GO:0051094 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051276 GO:0051570 GO:0051574 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060968 GO:0061085 GO:0061087 GO:0061458 GO:0065007 GO:0070827 GO:0070828 GO:0070829 GO:0071840 GO:0080090 GO:0090436 GO:0097159 GO:0098542 GO:0099402 GO:0140110 GO:1900109 GO:1900111 GO:1900363 GO:1901363 GO:1902275 GO:1903311 GO:1903506 GO:1905269 GO:2000024 GO:2000026 GO:2000112 GO:2000241 GO:2000243 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNMT1-RFD

Protein Analysis

1178

Amino Acids

132.48

Weight (kDa)

6.65

Isoelectric Point (pI)

43.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNMT1-RFD PF12047 7 - 89 1.9e-08 Cytosine specific DNA methyltransferase replication foci domain
PHD_NSD PF22908 233 - 301 2.5e-19 Histone-lysine N-methyltransferase NSD-like, PHD zinc finger
Mtase_EDM2 PF26055 682 - 852 5.2e-92 EDM2 methyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000343)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48090 AT5G48090 AT5G48090 AT5G55390 AT5G55390 AT5G55390
fragaria_vesca FvH4_3g23690 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160
malus_domestica MD03G1191000.v1.1 MD11G1207800.v1.1 MD11G1208000.v1.1
prunus_persica Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227600_v2.0.a1 Prupe.4G227700_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1
pyrus_communis pycom01g03400 pycom03g14420 pycom11g18100 pycom11g18140 pycom11g18150 pycom11g18160
rosa_chinensis RchiOBHm_Chr2g0145981 RchiOBHm_Chr4g0405241 RchiOBHm_Chr4g0405271 RchiOBHm_Chr5g0016201 RchiOBHm_Chr5g0041831 RchiOBHm_Chr5g0041871 RchiOBHm_Chr5g0041881 RchiOBHm_Chr5g0059241
rosa_laevigata RLG00000002117 RLG00000008861 RLG00000008862 RLG00000011000 RLG00000033216 RLG00000034097 RLG00000034101
rosa_multiflora Rmu_sc0000161.1_g000001 Rmu_sc0000861.1_g000040 Rmu_sc0007522.1_g000003 Rmu_sc0009443.1_g000015 Rmu_sc0022129.1_g000001 Rmu_sc0042315.1_g000001
rosa_roxburghii Rroxscaffold_1G00038660 Rroxscaffold_1G00038690 Rroxscaffold_5G00349560 Rroxscaffold_5G00349610 Rroxscaffold_6G00388370 Rroxscaffold_7G00188740
rosa_rugosa Rorug03G0199700 Rorug04G0055400 Rorug04G0055500 Rorug04G0055900 Rorug04G0056000 Rorug04G0056100 Rorug04G0056100 Rorug04G0056200 Rorug05G0195600 Rorug05G0195700 Rorug05G0195800 Rorug05G0195900 Rorug05G0196800 Rorug05G0385000
rosa_samantha Rh4AG129900 Rh4AG130100 Rh4BG124200 Rh4BG124400 Rh4BG124500 Rh4CG137200 Rh4CG137300 Rh4DG123500 Rh4DG123700 Rh5AG280800 Rh5AG281400 Rh5BG286700 Rh5BG287000 Rh5BG397800 Rh5CG318100 Rh5CG318600 Rh5CG421600 Rh5DG132300 Rh5DG132400 Rh5DG295400 Rh5DG295700 Rh7BG475300
rosa_wichuraiana Rw0G001330 Rw0G005730 Rw4G008670 Rw4G010510 Rw4G010540 Rw5G026450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 828, 1733, 2148
AasI GACNNNNNNGTC 1 cut(s) 839
Acc36I ACCTGC 2 cut(s) 1438, 3286
AccB1I GGYRCC 2 cut(s) 2084, 3334
AccI GTMKAC 2 cut(s) 2585, 2915
AciI CCGC 7 cut(s) 70, 1392, 2495, 2532, 3217, 3262, 3296
AcsI RAATTY 6 cut(s) 1190, 1331, 2730, 3406, 3436, 3449
AcuI CTGAAG 7 cut(s) 23, 122, 507, 732, 870, 1397, 1893
AdeI CACNNNGTG 1 cut(s) 208
AfaI GTAC 5 cut(s) 275, 1084, 1125, 3004, 3191
AfiI CCNNNNNNNGG 8 cut(s) 33, 402, 990, 2270, 2699, 2930, 3284, 3344
AflIII ACRYGT 3 cut(s) 890, 1977, 3300
AgeI ACCGGT 1 cut(s) 2918
AjiI CACGTC 1 cut(s) 1980
AjnI CCWGG 7 cut(s) 300, 442, 645, 2692, 2779, 3166, 3329
Alw21I GWGCWC 1 cut(s) 1897
Alw26I GTCTC 5 cut(s) 218, 623, 847, 2120, 3471
AlwNI CAGNNNCTG 4 cut(s) 46, 755, 1886, 2419
Ama87I CYCGRG 2 cut(s) 2684, 2893
AoxI GGCC 2 cut(s) 3259, 3341
ApeKI GCWGC 6 cut(s) 909, 1664, 1703, 2282, 3176, 3214
ApoI RAATTY 6 cut(s) 1190, 1331, 2730, 3406, 3436, 3449
AsiGI ACCGGT 1 cut(s) 2918
Asp700I GAANNNNTTC 5 cut(s) 155, 663, 1278, 1318, 2180
AspLEI GCGC 3 cut(s) 1646, 1974, 2656
AspS9I GGNCC 7 cut(s) 1180, 2529, 2921, 3170, 3259, 3342, 3380
AsuC2I CCSGG 4 cut(s) 2445, 3340, 3345, 3378
AsuHPI GGTGA 6 cut(s) 316, 623, 1538, 1901, 2660, 3377
AsuII TTCGAA 3 cut(s) 1228, 1834, 3398
AvaI CYCGRG 2 cut(s) 2684, 2893
AvaII GGWCC 5 cut(s) 1180, 2529, 2921, 3170, 3380
AxyI CCTNAGG 2 cut(s) 581, 1899
BaeGI GKGCMC 2 cut(s) 2973, 3337
BaeI ACNNNNGTAYC 2 cut(s) 2052, 2085
BamHI GGATCC 1 cut(s) 940
BanI GGYRCC 2 cut(s) 2084, 3334
BauI CACGAG 2 cut(s) 2974, 3250
BbsI GAAGAC 7 cut(s) 398, 495, 754, 1056, 1071, 1241, 1701
Bbv12I GWGCWC 1 cut(s) 1897
BbvCI CCTCAGC 1 cut(s) 756
BbvI GCAGC 6 cut(s) 896, 1676, 1690, 2294, 3188, 3226
BceAI ACGGC 2 cut(s) 1854, 3331
BcgI CGANNNNNNTGC 2 cut(s) 2788, 2822
BciT130I CCWGG 7 cut(s) 302, 444, 647, 2694, 2781, 3168, 3331
BciVI GTATCC 1 cut(s) 2401
BclI TGATCA 4 cut(s) 415, 1528, 2247, 2638
BcnI CCSGG 4 cut(s) 2445, 3340, 3345, 3378
BcoDI GTCTC 5 cut(s) 218, 623, 847, 2120, 3471
BfaI CTAG 3 cut(s) 1197, 1338, 1853
BfmI CTRYAG 5 cut(s) 1543, 1668, 2547, 3174, 3282
BfoI RGCGCY 1 cut(s) 2657
BfuAI ACCTGC 2 cut(s) 1438, 3286
BfuI GTATCC 1 cut(s) 2401
BisI GCNGC 8 cut(s) 910, 1665, 1704, 2283, 2495, 3177, 3215, 3262
BlsI GCNGC 8 cut(s) 911, 1666, 1705, 2284, 2496, 3178, 3216, 3263
Bme18I GGWCC 5 cut(s) 1180, 2529, 2921, 3170, 3380
BmeT110I CYCGRG 2 cut(s) 2684, 2893
BmgBI CACGTC 1 cut(s) 1980
BmgT120I GGNCC 7 cut(s) 1180, 2529, 2921, 3170, 3259, 3342, 3380
BmiI GGNNCC 8 cut(s) 733, 942, 1181, 2036, 2086, 2923, 3336, 3361
BmrI ACTGGG 1 cut(s) 2407
BmsI GCATC 9 cut(s) 337, 566, 1651, 1690, 1853, 2446, 2585, 3007, 3492
BmuI ACTGGG 1 cut(s) 2407
BpiI GAAGAC 7 cut(s) 398, 495, 754, 1056, 1071, 1241, 1701
BplI GAGNNNNNCTC 4 cut(s) 2876, 2908, 2887, 2919
BpmI CTGGAG 1 cut(s) 2533
Bpu10I CCTNAGC 2 cut(s) 756, 3265
Bpu14I TTCGAA 3 cut(s) 1228, 1834, 3398
BpuEI CTTGAG 3 cut(s) 878, 2336, 2346
BpuMI CCSGG 4 cut(s) 2445, 3340, 3345, 3378
BsaAI YACGTR 1 cut(s) 891
BsaI GGTCTC 1 cut(s) 623
BsaWI WCCGGW 1 cut(s) 2918
BsaXI ACNNNNNCTCC 2 cut(s) 2945, 2975
Bsc4I CCNNNNNNNGG 8 cut(s) 33, 402, 990, 2270, 2699, 2930, 3284, 3344
Bse118I RCCGGY 1 cut(s) 2918
Bse1I ACTGG 3 cut(s) 131, 2413, 3306
Bse21I CCTNAGG 2 cut(s) 581, 1899
Bse3DI GCAATG 4 cut(s) 805, 810, 2838, 2884
BseBI CCWGG 7 cut(s) 302, 444, 647, 2694, 2781, 3168, 3331
BseLI CCNNNNNNNGG 8 cut(s) 33, 402, 990, 2270, 2699, 2930, 3284, 3344
BseMI GCAATG 4 cut(s) 805, 810, 2838, 2884
BseNI ACTGG 3 cut(s) 131, 2413, 3306
BseRI GAGGAG 5 cut(s) 226, 724, 1022, 1073, 1690
BseSI GKGCMC 2 cut(s) 2973, 3337
BseXI GCAGC 6 cut(s) 896, 1676, 1690, 2294, 3188, 3226
BseYI CCCAGC 2 cut(s) 1433, 1502
BsgI GTGCAG 1 cut(s) 212
BshFI GGCC 2 cut(s) 3261, 3343
BshNI GGYRCC 2 cut(s) 2084, 3334
BshTI ACCGGT 1 cut(s) 2918
BsiHKAI GWGCWC 1 cut(s) 1897
BsiHKCI CYCGRG 2 cut(s) 2684, 2893
BsiSI CCGG 6 cut(s) 231, 2445, 2919, 3340, 3345, 3378
BslFI GGGAC 4 cut(s) 1193, 1279, 2839, 2907
BslI CCNNNNNNNGG 8 cut(s) 33, 402, 990, 2270, 2699, 2930, 3284, 3344
BsmAI GTCTC 5 cut(s) 218, 623, 847, 2120, 3471
BsmBI CGTCTC 2 cut(s) 2120, 3471
BsmFI GGGAC 4 cut(s) 1193, 1279, 2839, 2907
BsmI GAATGC 4 cut(s) 616, 1974, 2742, 2770
BsnI GGCC 2 cut(s) 3261, 3343
Bso31I GGTCTC 1 cut(s) 623
BsoBI CYCGRG 2 cut(s) 2684, 2893
Bsp119I TTCGAA 3 cut(s) 1228, 1834, 3398
Bsp1286I GDGCHC 3 cut(s) 1897, 2973, 3337
Bsp1407I TGTACA 1 cut(s) 3002
Bsp19I CCATGG 1 cut(s) 3363
BspACI CCGC 7 cut(s) 70, 1392, 2495, 2532, 3217, 3262, 3296
BspANI GGCC 2 cut(s) 3261, 3343
BspLI GGNNCC 8 cut(s) 733, 942, 1181, 2036, 2086, 2923, 3336, 3361
BspMAI CTGCAG 1 cut(s) 3178
BspMI ACCTGC 2 cut(s) 1438, 3286
BspQI GCTCTTC 1 cut(s) 146
BspT104I TTCGAA 3 cut(s) 1228, 1834, 3398
BspT107I GGYRCC 2 cut(s) 2084, 3334
BspTNI GGTCTC 1 cut(s) 623
BsrDI GCAATG 4 cut(s) 805, 810, 2838, 2884
BsrFI RCCGGY 1 cut(s) 2918
BsrGI TGTACA 1 cut(s) 3002
BsrI ACTGG 3 cut(s) 131, 2413, 3306
BssAI RCCGGY 1 cut(s) 2918
BssNAI GTATAC 1 cut(s) 2586
BssSI CACGAG 2 cut(s) 2974, 3250
BssT1I CCWWGG 3 cut(s) 634, 2995, 3363
Bst1107I GTATAC 1 cut(s) 2586
Bst2BI CACGAG 2 cut(s) 2974, 3250
Bst2UI CCWGG 7 cut(s) 302, 444, 647, 2694, 2781, 3168, 3331
Bst6I CTCTTC 6 cut(s) 146, 381, 1035, 1050, 2118, 2189
BstAPI GCANNNNNTGC 2 cut(s) 1969, 3017
BstAUI TGTACA 1 cut(s) 3002
BstBAI YACGTR 1 cut(s) 891
BstBI TTCGAA 3 cut(s) 1228, 1834, 3398
BstC8I GCNNGC 3 cut(s) 1001, 2751, 2801
BstDSI CCRYGG 1 cut(s) 3363
BstENI CCTNNNNNAGG 2 cut(s) 988, 3282
BstH2I RGCGCY 1 cut(s) 2657
BstHHI GCGC 3 cut(s) 1646, 1974, 2656
BstMAI GTCTC 5 cut(s) 218, 623, 847, 2120, 3471
BstMWI GCNNNNNNNGC 5 cut(s) 1892, 1969, 2288, 2776, 3017
BstNI CCWGG 7 cut(s) 302, 444, 647, 2694, 2781, 3168, 3331
BstNSI RCATGY 3 cut(s) 59, 2803, 3304
BstSFI CTRYAG 5 cut(s) 1543, 1668, 2547, 3174, 3282
BstSLI GKGCMC 2 cut(s) 2973, 3337
BstV1I GCAGC 6 cut(s) 896, 1676, 1690, 2294, 3188, 3226
BstV2I GAAGAC 7 cut(s) 398, 495, 754, 1056, 1071, 1241, 1701
BstX2I RGATCY 4 cut(s) 940, 2447, 3269, 3493
BstXI CCANNNNNNTGG 2 cut(s) 2561, 3490
BstYI RGATCY 4 cut(s) 940, 2447, 3269, 3493
BstZ17I GTATAC 1 cut(s) 2586
Bsu36I CCTNAGG 2 cut(s) 581, 1899
BsuI GTATCC 1 cut(s) 2401
BsuRI GGCC 2 cut(s) 3261, 3343
BtgI CCRYGG 1 cut(s) 3363
BtrI CACGTC 1 cut(s) 1980
BtsI GCAGTG 3 cut(s) 21, 1873, 2571
BtsIMutI CAGTG 4 cut(s) 21, 195, 1873, 2571
BveI ACCTGC 2 cut(s) 1438, 3286
Cac8I GCNNGC 3 cut(s) 1001, 2751, 2801
CaiI CAGNNNCTG 4 cut(s) 46, 755, 1886, 2419
CfoI GCGC 3 cut(s) 1646, 1974, 2656
Cfr10I RCCGGY 1 cut(s) 2918
Cfr13I GGNCC 7 cut(s) 1180, 2529, 2921, 3170, 3259, 3342, 3380
CseI GACGC 2 cut(s) 491, 1695
CsiI ACCWGGT 1 cut(s) 300
Csp6I GTAC 5 cut(s) 274, 1083, 1124, 3003, 3190
CspAI ACCGGT 1 cut(s) 2918
CviQI GTAC 5 cut(s) 274, 1083, 1124, 3003, 3190
DraI TTTAAA 1 cut(s) 2610
DraIII CACNNNGTG 1 cut(s) 208
DrdI GACNNNNNNGTC 1 cut(s) 839
DseDI GACNNNNNNGTC 1 cut(s) 839
Eam1104I CTCTTC 6 cut(s) 146, 381, 1035, 1050, 2118, 2189
EarI CTCTTC 6 cut(s) 146, 381, 1035, 1050, 2118, 2189
Eco130I CCWWGG 3 cut(s) 634, 2995, 3363
Eco31I GGTCTC 1 cut(s) 623
Eco32I GATATC 4 cut(s) 102, 1296, 1362, 3290
Eco47I GGWCC 5 cut(s) 1180, 2529, 2921, 3170, 3380
Eco57I CTGAAG 7 cut(s) 23, 122, 507, 732, 870, 1397, 1893
Eco81I CCTNAGG 2 cut(s) 581, 1899
Eco88I CYCGRG 2 cut(s) 2684, 2893
EcoNI CCTNNNNNAGG 2 cut(s) 988, 3282
EcoRI GAATTC 1 cut(s) 1331
EcoRII CCWGG 7 cut(s) 300, 442, 645, 2692, 2779, 3166, 3329
EcoRV GATATC 4 cut(s) 102, 1296, 1362, 3290
EcoT14I CCWWGG 3 cut(s) 634, 2995, 3363
EcoT22I ATGCAT 2 cut(s) 1965, 3022
ErhI CCWWGG 3 cut(s) 634, 2995, 3363
Esp3I CGTCTC 2 cut(s) 2120, 3471
FalI AAGNNNNNCTT 2 cut(s) 2126, 2158
FaqI GGGAC 4 cut(s) 1193, 1279, 2839, 2907
FbaI TGATCA 4 cut(s) 415, 1528, 2247, 2638
FblI GTMKAC 2 cut(s) 2585, 2915
Fnu4HI GCNGC 8 cut(s) 910, 1665, 1704, 2283, 2495, 3177, 3215, 3262
Fsp4HI GCNGC 8 cut(s) 910, 1665, 1704, 2283, 2495, 3177, 3215, 3262
FspBI CTAG 3 cut(s) 1197, 1338, 1853
GlaI GCGC 3 cut(s) 1645, 1973, 2655
GluI GCNGC 8 cut(s) 910, 1665, 1704, 2283, 2495, 3177, 3215, 3262
GsaI CCCAGC 2 cut(s) 1437, 1506
GsuI CTGGAG 1 cut(s) 2533
HaeII RGCGCY 1 cut(s) 2657
HaeIII GGCC 2 cut(s) 3261, 3343
HapII CCGG 6 cut(s) 231, 2445, 2919, 3340, 3345, 3378
HgaI GACGC 2 cut(s) 491, 1695
HhaI GCGC 3 cut(s) 1646, 1974, 2656
Hin6I GCGC 3 cut(s) 1644, 1972, 2654
HinP1I GCGC 3 cut(s) 1644, 1972, 2654
HincII GTYRAC 3 cut(s) 692, 1638, 3350
HindII GTYRAC 3 cut(s) 692, 1638, 3350
HindIII AAGCTT 1 cut(s) 1316
HpaII CCGG 6 cut(s) 231, 2445, 2919, 3340, 3345, 3378
HphI GGTGA 6 cut(s) 316, 623, 1538, 1901, 2660, 3377
Hpy99I CGWCG 1 cut(s) 506
HpyCH4IV ACGT 3 cut(s) 890, 1979, 3039
HpyF10VI GCNNNNNNNGC 5 cut(s) 1892, 1969, 2288, 2776, 3017
HpySE526I ACGT 3 cut(s) 890, 1979, 3039
HspAI GCGC 3 cut(s) 1644, 1972, 2654
Ksp22I TGATCA 4 cut(s) 415, 1528, 2247, 2638
LguI GCTCTTC 1 cut(s) 146
Lsp1109I GCAGC 6 cut(s) 896, 1676, 1690, 2294, 3188, 3226
LweI GCATC 9 cut(s) 337, 566, 1651, 1690, 1853, 2446, 2585, 3007, 3492
MabI ACCWGGT 1 cut(s) 300
MaeI CTAG 3 cut(s) 1197, 1338, 1853
MaeII ACGT 3 cut(s) 890, 1979, 3039
MaeIII GTNAC 6 cut(s) 1842, 2226, 2238, 2488, 2564, 3208
MfeI CAATTG 3 cut(s) 1821, 2243, 2471
MflI RGATCY 4 cut(s) 940, 2447, 3269, 3493
MhlI GDGCHC 3 cut(s) 1897, 2973, 3337
MmeI TCCRAC 2 cut(s) 522, 3213
Mph1103I ATGCAT 2 cut(s) 1965, 3022
MroXI GAANNNNTTC 5 cut(s) 155, 663, 1278, 1318, 2180
MslI CAYNNNNRTG 4 cut(s) 1799, 2043, 2105, 3254
MspA1I CMGCKG 3 cut(s) 2534, 3179, 3217
MspI CCGG 6 cut(s) 231, 2445, 2919, 3340, 3345, 3378
MunI CAATTG 3 cut(s) 1821, 2243, 2471
Mva1269I GAATGC 4 cut(s) 616, 1974, 2742, 2770
MvaI CCWGG 7 cut(s) 302, 444, 647, 2694, 2781, 3168, 3331
MwoI GCNNNNNNNGC 5 cut(s) 1892, 1969, 2288, 2776, 3017
NciI CCSGG 4 cut(s) 2445, 3340, 3345, 3378
NcoI CCATGG 1 cut(s) 3363
NlaIV GGNNCC 8 cut(s) 733, 942, 1181, 2036, 2086, 2923, 3336, 3361
NmeAIII GCCGAG 1 cut(s) 171
NmuCI GTSAC 3 cut(s) 2238, 2564, 3208
NsiI ATGCAT 2 cut(s) 1965, 3022
NspI RCATGY 3 cut(s) 59, 2803, 3304
NspV TTCGAA 3 cut(s) 1228, 1834, 3398
PaeI GCATGC 1 cut(s) 2803
PaeR7I CTCGAG 1 cut(s) 2893
PasI CCCWGGG 1 cut(s) 3330
PciI ACATGT 1 cut(s) 3300
PciSI GCTCTTC 1 cut(s) 146
PcsI WCGNNNNNNNCGW 2 cut(s) 489, 498
PctI GAATGC 4 cut(s) 616, 1974, 2742, 2770
PdmI GAANNNNTTC 5 cut(s) 155, 663, 1278, 1318, 2180
PinAI ACCGGT 1 cut(s) 2918
PkrI GCNGC 8 cut(s) 911, 1666, 1705, 2284, 2496, 3178, 3216, 3263
Ppu21I YACGTR 1 cut(s) 891
PscI ACATGT 1 cut(s) 3300
PsiI TTATAA 3 cut(s) 828, 1733, 2148
Psp6I CCWGG 7 cut(s) 300, 442, 645, 2692, 2779, 3166, 3329
PspFI CCCAGC 2 cut(s) 1433, 1502
PspGI CCWGG 7 cut(s) 300, 442, 645, 2692, 2779, 3166, 3329
PspN4I GGNNCC 8 cut(s) 733, 942, 1181, 2036, 2086, 2923, 3336, 3361
PspPI GGNCC 7 cut(s) 1180, 2529, 2921, 3170, 3259, 3342, 3380
PspXI VCTCGAGB 1 cut(s) 2893
PsrI GAACNNNNNNTAC 2 cut(s) 1095, 1127
PstI CTGCAG 1 cut(s) 3178
PstNI CAGNNNCTG 4 cut(s) 46, 755, 1886, 2419
PsuI RGATCY 4 cut(s) 940, 2447, 3269, 3493
PvuII CAGCTG 1 cut(s) 3179
RsaI GTAC 5 cut(s) 275, 1084, 1125, 3004, 3191
RsaNI GTAC 5 cut(s) 274, 1083, 1124, 3003, 3190
RseI CAYNNNNRTG 4 cut(s) 1799, 2043, 2105, 3254
SapI GCTCTTC 1 cut(s) 146
SatI GCNGC 8 cut(s) 910, 1665, 1704, 2283, 2495, 3177, 3215, 3262
Sau96I GGNCC 7 cut(s) 1180, 2529, 2921, 3170, 3259, 3342, 3380
SduI GDGCHC 3 cut(s) 1897, 2973, 3337
SexAI ACCWGGT 1 cut(s) 300
SfaNI GCATC 9 cut(s) 337, 566, 1651, 1690, 1853, 2446, 2585, 3007, 3492
SfcI CTRYAG 5 cut(s) 1543, 1668, 2547, 3174, 3282
Sfr274I CTCGAG 1 cut(s) 2893
SfuI TTCGAA 3 cut(s) 1228, 1834, 3398
SinI GGWCC 5 cut(s) 1180, 2529, 2921, 3170, 3380
SlaI CTCGAG 1 cut(s) 2893
SmiMI CAYNNNNRTG 4 cut(s) 1799, 2043, 2105, 3254
SmlI CTYRAG 4 cut(s) 857, 2315, 2361, 2893
SmoI CTYRAG 4 cut(s) 857, 2315, 2361, 2893
SphI GCATGC 1 cut(s) 2803
SsiI CCGC 7 cut(s) 70, 1392, 2495, 2532, 3217, 3262, 3296
SspI AATATT 1 cut(s) 1614
SspMI CTAG 3 cut(s) 1197, 1338, 1853
StyI CCWWGG 3 cut(s) 634, 2995, 3363
TaiI ACGT 3 cut(s) 893, 1982, 3042
TaqI TCGA 7 cut(s) 90, 159, 1228, 1754, 1834, 2894, 3398
TaqII GACCGA 2 cut(s) 1253, 2610
TatI WGTACW 2 cut(s) 1123, 3002
TauI GCSGC 2 cut(s) 2497, 3264
TscAI CASTG 4 cut(s) 28, 195, 1873, 2578
TseFI GTSAC 3 cut(s) 2238, 2564, 3208
TseI GCWGC 6 cut(s) 909, 1664, 1703, 2282, 3176, 3214
Tsp45I GTSAC 3 cut(s) 2238, 2564, 3208
TspGWI ACGGA 2 cut(s) 479, 1861
TspRI CASTG 4 cut(s) 28, 195, 1873, 2578
VpaK11BI GGWCC 5 cut(s) 1180, 2529, 2921, 3170, 3380
XagI CCTNNNNNAGG 2 cut(s) 988, 3282
XapI RAATTY 6 cut(s) 1190, 1331, 2730, 3406, 3436, 3449
XceI RCATGY 3 cut(s) 59, 2803, 3304
XcmI CCANNNNNNNNNTGG 3 cut(s) 1780, 2508, 3164
XhoI CTCGAG 1 cut(s) 2893
XmiI GTMKAC 2 cut(s) 2585, 2915
XmnI GAANNNNTTC 5 cut(s) 155, 663, 1278, 1318, 2180
XspI CTAG 3 cut(s) 1197, 1338, 1853
Zsp2I ATGCAT 2 cut(s) 1965, 3022
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.