pycom03g14420

metal ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0000343
Physical Location & Seq
Forward (+)
15748019 .. 15759703
11685 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g14420.11

Sequence Viewer

Length: 3678 bp
ATGGAATCGTCAGACGATGAGGCAGAGGCGGTGCCACAATCCGTTTCGAATTACCATTTTGTAGATGACAAGGACGAACCCATCTCATTTCATGTTTTGCCAATTCAGTGGAGTGAGGGTGAGAGGCAAGATGGGAGGAAACTGGACATATTTGTGCGTGGCACTGCCGATAATGGGCTTCAGAAACTGTATAAGCATGTTATCGCATGGCGGTTTGATCTTTCCAATTTCGATCCTGAGATATCGGTGCTCTCTAAGGAAAATAACTGGCTCAAGCTTCAGAAGCCGAGGAAGAGTTTCGAGGAAGTAATAAGGTCAATCTTGCTAACAGTGCACTGCCTTCACTATGTGAGGAGAAATCCTGAGACATCCGGCAAATCTCTGTGGGATCACTTATCTAAAGTTTTCAGCTCGTATGAGGTCAGGCCTTCTCAGAATGACCTGGTGAATTATATTCCTCTAATTAGTGAAGCTGTTAAACGGGATGATGCATTGGCAAAGTGCAAGTTTTTGGTCAACTTTCTTGAAGAGAAGCCCACCAAGAGGAAACTGCATGATGAGGACATTCAAGCTACAACAAAACCTGGATTTATAGTTGATGATATGGAAGAAGATATGATTGATGCTGAAGACGAGTCCAGTGACGATGATAACCTGTTTGATTCTGTTTGTGCGTTTTGTGATAATGGTGGCGATCTTTTGTGTTGTGAAGGGAGATGCCTAAGGTCATTTCACGCAACTGTAGAGGATGGTGAGGATTCTATGTGTGAAACCCTTGGCTTTACCCAGGATGAAGTAGACGCGATTCAGAACTTCTTCTGCAAGAACTGTCAGTATAAACAGCATCAGTGCTATGCTTGTGGGAAGCTAGGCTCCTCTGATAAATCTTCAGGGGCTGAGGTCTTCCCCTGTGTTTCTGCAACCTGTGGTCAATTTTACCATCCACGTTGCATTGCAAAACTCATTTATAAAGACAATGGAGTCTCTGCTGAAGAACTGGAGAAAAAAATTGCTATGGGTGAATCTTTTACATGTCCGATTCATAAATGCTGCGTTTGTAAACAAGGAGAGAACAAGAAGAATCCTCAGTTGCGGTTTGCTGTGTGTCGGCGTTGTCCCAAATCCTACCACAAGAAATGCTTGCCGGAGGATATTGTATTTGAGAAAACAGAGAAAACAGAGGAAGATGAGGAGGAAGACGAAGAGGATGAAGGTACAACACCAAGGGCTTGGGAAGGACTATTACCAAACCGTGTACTAATATATTGCACAAAACATGAGATAATTAAAGACATTGGAACGCCAATAAGGGACCATGTAACATTCCCTGATGTTGGAGAAAAGAGGACCAGTTTTGTTCGAAAGAAGACTGATTTTGTAGGGAAAAAGAAAACACGGACATTAGAATCCCTTCAAGATAGAGAAAAATCTGTGAAAAATAAGAGAAGCCTTTCCGCTGAAGAATTCTGTAGGGGCCAAACTGCTCCTACGATATCTAAAGAGAAGCTGAAGTCATCTTCTGCTGCAAAAGTGGGTGGTAACAGAATTAGTAAAAAACTACCTTCTGGGTTAGATACATCAAGAAAGGTGAAAGCGAATAGTGCTTTGAAAAAGGAAGCTAAGGTTTCTGTGGCTGAGGAACAAAAGACCTCATTTGGTGATCAGCTATATGCCTATATGAATAGGTCTAATCAAGTGAAGTCAGGTAAGCAGGGCAAACCTGATGGGGAGTGTGGTTTGGCAATAGGTAATCCTGCCTCAAAAACGTTAATCAGTGCACCACCATCTTTAGATGCAGCTACAGAGAGGAGATTACTGGCTTTAATGAAAGATGCCGCATCTTCAATAACCCTGGAAGATGTTATAAGAAAGCGGCAGCGCACAGTTCCATCAACTCATCAATCCTCGTCCAAAAATGCTGTGGATAGGAACATTACACTGGGAAAGGTAGAAGGCTCAGTTGAGGCTGTTCGAACAGCATTACGGAAACTAGAGGAAGGATGCAGTACTGAAGATTCTGAGGCTGTTTGTGCTCCTGAGGTGGTTCACCAGATTTACAAGTGGAAGAACAAGCTGAGAGTATATCTTGCACCTTTTTTGCACGGAATGCGCTACACGTCCTTTGGTCGTCATTTTACAAAGGTGGACAAATTAGAAGAGATTGCTGATAGGCTGCATTGGTACGTAAAAGATGGAGATACGATAGTGGACTTCTGTTGTGGTGCCAATGATTTCAGCATCGTAATGAAGAAAAAGCTTGAAGAGACGGGGAAGAACTGCTTTTATAAAAACTATGACTTAATTCAACCGAAGAACGATTTCTGTTTTGAGAAGAGAGATTGGATGAAAGTACAACCAAAGGAGTTACCAAAAGGGTCACAATTGATCATGGGGTTGAACCCTCCTTTCGGAGTTAAAGCAGCTCTGGCAAACAAGTTTATCGACAAGGCTCTTGAGTTCGATCCAAAGCTCCTTATTCTTATTGTTCCACCAGAAACTCAAAGGTTAAATGAAAAGAAATCGCCATATGATCTAATTTGGGAGGATAACCAGTTTCTGTCTGGAAAGTCTTTTTATCTCCCGGGATCTGTTGATGTGAATGACAAACAAATGGATCAATGGAATGTAACGCCGCCACCACTCTATCTCTGGAGTCGTCCTGATTGGTCTGCTAACATCAAGGCTATAGCTCAAGAGCATGGTCACATGTCTGCATCACAAGGGTATATGAAAGAGCATTCTGACTCTCTAAATCACGGTCGGTCAATAGGTAATAATGATCAGTATGGTGAAGCACCTATGCTGATTGATGATGATGGCATAAAAACCGATAGTCCCAAAGATGTTGAAGGTGGGGCTGTTGTGACTGAGGAACACAGAGAAAGCTCCTGTGAGAACAGTGGTGACATAGGTGGCAACGAGAGTCCTGGGGATGGTAACAACATTGATGAGACATGCAGGGAAATTCTGCCACGCATTGAGCCTGCTGAAAAAGGAGATCAGCACTCTGAGCCTAGCAATTCTGGTTCAAGCGTGAAGTACGGAACTACTTATGGTGGGACAAAGGTTAACATAGCTGATGACAGAGGTAGAAGAAGCTTATCCAGGAGCAGTGATGAGCCTTACTTGAGTCTGACTCATAGATGGTCCGCTGGTCCCAGTTCTGGTTATAGGGCTACAAACTTGGAGGAACCGTTTGTGGGGCACATGAGAGACAGATCGGATACCCTTGGATATAGACCATGCTTGAATGAGGTGGAAGATCCATTTAGGAGGGAGTCTGACGTACGTTCTCAGATTCGGCTTTATGGTCAGCAAGATTTTGGTCCTTTAAGAAGTAATTATCTAGTGGGTCAGGATTCTGTAAGTGCACAGATTGGATCGTATTCATCTCCTTACAGCCATAGTCATCTTGGTCCGACAGCCGAGTCATCTTACAGAATGAACACGTCCGCCATGCAGCGGTATGCACCTCGGTTGGATGAACTGAACCACACGAGGATGGGTGGCTTAGGATCTGAACCTGCTTTAGGATACGAACCGCACATGTTCAGCAGCACCGGCACTTTTGATCCCAGGGCACCCCGGCCCGGTCAACATGGTGGTTCCATGGGTTTTGCTCCCGGTCCTCACCAAAGCTATTCGAACCAAAATTCAGCAGGTTGGTTGAATGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006325 GO:0006355 GO:0006950 GO:0006952 GO:0006996 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0009605 GO:0009607 GO:0009620 GO:0009791 GO:0009889 GO:0009893 GO:0009909 GO:0009911 GO:0009987 GO:0010228 GO:0010468 GO:0010556 GO:0010604 GO:0010638 GO:0016043 GO:0019219 GO:0019222 GO:0022414 GO:0023052 GO:0030154 GO:0031056 GO:0031058 GO:0031060 GO:0031062 GO:0031323 GO:0031325 GO:0031326 GO:0031399 GO:0031401 GO:0031440 GO:0031935 GO:0031937 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033043 GO:0033044 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0043954 GO:0044030 GO:0044424 GO:0044464 GO:0048366 GO:0048367 GO:0048468 GO:0048518 GO:0048522 GO:0048580 GO:0048582 GO:0048608 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0048869 GO:0050684 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051052 GO:0051094 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051276 GO:0051570 GO:0051574 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060968 GO:0061085 GO:0061087 GO:0061458 GO:0065007 GO:0070827 GO:0070828 GO:0070829 GO:0071840 GO:0080090 GO:0090436 GO:0097159 GO:0098542 GO:0099402 GO:0140110 GO:1900109 GO:1900111 GO:1900363 GO:1901363 GO:1902275 GO:1903311 GO:1903506 GO:1905269 GO:2000024 GO:2000026 GO:2000112 GO:2000241 GO:2000243 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNMT1-RFD

Protein Analysis

1226

Amino Acids

137.54

Weight (kDa)

5.92

Isoelectric Point (pI)

46.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000343)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48090 AT5G48090 AT5G48090 AT5G55390 AT5G55390 AT5G55390
fragaria_vesca FvH4_3g23690 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160
malus_domestica MD03G1191000.v1.1 MD11G1207800.v1.1 MD11G1208000.v1.1
prunus_persica Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227600_v2.0.a1 Prupe.4G227700_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1
pyrus_communis pycom01g03400 pycom03g14420 pycom11g18100 pycom11g18140 pycom11g18150 pycom11g18160
rosa_chinensis RchiOBHm_Chr2g0145981 RchiOBHm_Chr4g0405241 RchiOBHm_Chr4g0405271 RchiOBHm_Chr5g0016201 RchiOBHm_Chr5g0041831 RchiOBHm_Chr5g0041871 RchiOBHm_Chr5g0041881 RchiOBHm_Chr5g0059241
rosa_laevigata RLG00000002117 RLG00000008861 RLG00000008862 RLG00000011000 RLG00000033216 RLG00000034097 RLG00000034101
rosa_multiflora Rmu_sc0000161.1_g000001 Rmu_sc0000861.1_g000040 Rmu_sc0007522.1_g000003 Rmu_sc0009443.1_g000015 Rmu_sc0022129.1_g000001 Rmu_sc0042315.1_g000001
rosa_roxburghii Rroxscaffold_1G00038660 Rroxscaffold_1G00038690 Rroxscaffold_5G00349560 Rroxscaffold_5G00349610 Rroxscaffold_6G00388370 Rroxscaffold_7G00188740
rosa_rugosa Rorug03G0199700 Rorug04G0055400 Rorug04G0055500 Rorug04G0055900 Rorug04G0056000 Rorug04G0056100 Rorug04G0056100 Rorug04G0056200 Rorug05G0195600 Rorug05G0195700 Rorug05G0195800 Rorug05G0195900 Rorug05G0196800 Rorug05G0385000
rosa_samantha Rh4AG129900 Rh4AG130100 Rh4BG124200 Rh4BG124400 Rh4BG124500 Rh4CG137200 Rh4CG137300 Rh4DG123500 Rh4DG123700 Rh5AG280800 Rh5AG281400 Rh5BG286700 Rh5BG287000 Rh5BG397800 Rh5CG318100 Rh5CG318600 Rh5CG421600 Rh5DG132300 Rh5DG132400 Rh5DG295400 Rh5DG295700 Rh7BG475300
rosa_wichuraiana Rw0G001330 Rw0G005730 Rw4G008670 Rw4G010510 Rw4G010540 Rw5G026450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 969, 1865, 2286
AasI GACNNNNNNGTC 2 cut(s) 980, 3427
Acc36I ACCTGC 2 cut(s) 3532, 3650
AccB1I GGYRCC 3 cut(s) 31, 2222, 3580
AccI GTMKAC 1 cut(s) 798
AccII CGCG 1 cut(s) 803
AclI AACGTT 1 cut(s) 1766
AclWI GGATC 9 cut(s) 227, 396, 2456, 2593, 2622, 3257, 3388, 3523, 3566
AcsI RAATTY 3 cut(s) 1463, 2964, 3652
AcuI CTGAAG 8 cut(s) 164, 263, 648, 873, 1011, 1479, 1529, 2031
AdeI CACNNNGTG 1 cut(s) 349
AfaI GTAC 7 cut(s) 1216, 1257, 2008, 2183, 2352, 3041, 3288
AflIII ACRYGT 5 cut(s) 1031, 2115, 2706, 3447, 3546
AjiI CACGTC 2 cut(s) 2118, 3450
AjnI CCWGG 7 cut(s) 441, 583, 786, 1851, 2926, 3104, 3575
Alw21I GWGCWC 5 cut(s) 252, 336, 1780, 2035, 3373
Alw26I GTCTC 5 cut(s) 359, 988, 2258, 2945, 3207
Alw44I GTGCAC 3 cut(s) 332, 1776, 3369
AlwI GGATC 9 cut(s) 227, 396, 2456, 2593, 2622, 3257, 3388, 3523, 3566
AlwNI CAGNNNCTG 3 cut(s) 187, 896, 2557
Ama87I CYCGRG 1 cut(s) 2581
AoxI GGCC 3 cut(s) 425, 1474, 3587
ApaLI GTGCAC 3 cut(s) 332, 1776, 3369
ApeKI GCWGC 8 cut(s) 1050, 1523, 1796, 1876, 2173, 2420, 3460, 3555
ApoI RAATTY 3 cut(s) 1463, 2964, 3652
Asp700I GAANNNNTTC 5 cut(s) 296, 815, 1410, 1450, 2318
AspLEI GCGC 2 cut(s) 1881, 2112
AspS9I GGNCC 9 cut(s) 1312, 1347, 1474, 3147, 3155, 3326, 3416, 3588, 3626
AsuC2I CCSGG 5 cut(s) 2582, 2583, 3586, 3591, 3624
AsuII TTCGAA 4 cut(s) 47, 1360, 1972, 3644
AvaI CYCGRG 1 cut(s) 2581
AvaII GGWCC 7 cut(s) 1312, 1347, 3147, 3155, 3326, 3416, 3626
AxyI CCTNAGG 2 cut(s) 722, 2037
BaeGI GKGCMC 5 cut(s) 336, 1780, 3207, 3373, 3583
BaeI ACNNNNGTAYC 2 cut(s) 2190, 2223
BanI GGYRCC 3 cut(s) 31, 2222, 3580
BarI GAAGNNNNNNTAC 2 cut(s) 1227, 1259
BauI CACGAG 1 cut(s) 3496
BbsI GAAGAC 4 cut(s) 636, 895, 1203, 1373
Bbv12I GWGCWC 5 cut(s) 252, 336, 1780, 2035, 3373
BbvCI CCTCAGC 2 cut(s) 897, 1635
BbvI GCAGC 8 cut(s) 1037, 1510, 1808, 1888, 2160, 2432, 3472, 3567
BciT130I CCWGG 7 cut(s) 443, 585, 788, 1853, 2928, 3106, 3577
BciVI GTATCC 2 cut(s) 3217, 3527
BclI TGATCA 3 cut(s) 1660, 2385, 2779
BcnI CCSGG 5 cut(s) 2582, 2583, 3586, 3591, 3624
BcoDI GTCTC 5 cut(s) 359, 988, 2258, 2945, 3207
BfaI CTAG 4 cut(s) 869, 1991, 3015, 3347
BfmI CTRYAG 4 cut(s) 741, 1468, 1800, 2685
BfuAI ACCTGC 2 cut(s) 3532, 3650
BfuI GTATCC 2 cut(s) 3217, 3527
BmcAI AGTACT 1 cut(s) 2008
Bme18I GGWCC 7 cut(s) 1312, 1347, 3147, 3155, 3326, 3416, 3626
BmeT110I CYCGRG 1 cut(s) 2581
BmgBI CACGTC 2 cut(s) 2118, 3450
BmgT120I GGNCC 9 cut(s) 1312, 1347, 1474, 3147, 3155, 3326, 3416, 3588, 3626
BmiI GGNNCC 9 cut(s) 33, 874, 1313, 1475, 2224, 3157, 3192, 3582, 3607
BmrI ACTGGG 2 cut(s) 1949, 3153
BmuI ACTGGG 2 cut(s) 1949, 3153
BpiI GAAGAC 4 cut(s) 636, 895, 1203, 1373
BplI GAGNNNNNCTC 2 cut(s) 3121, 3153
BpmI CTGGAG 2 cut(s) 1019, 2671
Bpu10I CCTNAGC 4 cut(s) 897, 1620, 1635, 3511
Bpu14I TTCGAA 4 cut(s) 47, 1360, 1972, 3644
BpuEI CTTGAG 4 cut(s) 257, 2474, 2676, 3148
BpuMI CCSGG 5 cut(s) 2582, 2583, 3586, 3591, 3624
BsaAI YACGTR 1 cut(s) 2185
BsaXI ACNNNNNCTCC 4 cut(s) 103, 133, 3179, 3209
Bse118I RCCGGY 1 cut(s) 3560
Bse1I ACTGG 9 cut(s) 147, 272, 639, 1002, 1350, 1821, 1944, 2551, 3159
Bse21I CCTNAGG 2 cut(s) 722, 2037
Bse3DI GCAATG 1 cut(s) 951
BseBI CCWGG 7 cut(s) 443, 585, 788, 1853, 2928, 3106, 3577
BseMI GCAATG 1 cut(s) 951
BseNI ACTGG 9 cut(s) 147, 272, 639, 1002, 1350, 1821, 1944, 2551, 3159
BseRI GAGGAG 4 cut(s) 367, 865, 1205, 1822
BseSI GKGCMC 5 cut(s) 336, 1780, 3207, 3373, 3583
BseXI GCAGC 8 cut(s) 1037, 1510, 1808, 1888, 2160, 2432, 3472, 3567
Bsh1236I CGCG 1 cut(s) 803
Bsh1285I CGRYCG 1 cut(s) 2761
BshFI GGCC 3 cut(s) 427, 1476, 3589
BshNI GGYRCC 3 cut(s) 31, 2222, 3580
BsiEI CGRYCG 1 cut(s) 2761
BsiHKAI GWGCWC 5 cut(s) 252, 336, 1780, 2035, 3373
BsiHKCI CYCGRG 1 cut(s) 2581
BsiSI CCGG 7 cut(s) 372, 1145, 2582, 3561, 3586, 3591, 3624
BsiWI CGTACG 1 cut(s) 3286
BslFI GGGAC 5 cut(s) 1101, 1325, 2820, 3073, 3141
BsmAI GTCTC 5 cut(s) 359, 988, 2258, 2945, 3207
BsmBI CGTCTC 1 cut(s) 2258
BsmFI GGGAC 5 cut(s) 1101, 1325, 2820, 3073, 3141
BsmI GAATGC 2 cut(s) 2112, 2737
BsnI GGCC 3 cut(s) 427, 1476, 3589
BsoBI CYCGRG 1 cut(s) 2581
Bsp119I TTCGAA 4 cut(s) 47, 1360, 1972, 3644
Bsp1286I GDGCHC 7 cut(s) 252, 336, 1780, 2035, 3207, 3373, 3583
Bsp19I CCATGG 1 cut(s) 3609
BspANI GGCC 3 cut(s) 427, 1476, 3589
BspFNI CGCG 1 cut(s) 803
BspLI GGNNCC 9 cut(s) 33, 874, 1313, 1475, 2224, 3157, 3192, 3582, 3607
BspMI ACCTGC 2 cut(s) 3532, 3650
BspPI GGATC 9 cut(s) 227, 396, 2456, 2593, 2622, 3257, 3388, 3523, 3566
BspT104I TTCGAA 4 cut(s) 47, 1360, 1972, 3644
BspT107I GGYRCC 3 cut(s) 31, 2222, 3580
BsrDI GCAATG 1 cut(s) 951
BsrFI RCCGGY 1 cut(s) 3560
BsrI ACTGG 9 cut(s) 147, 272, 639, 1002, 1350, 1821, 1944, 2551, 3159
BssAI RCCGGY 1 cut(s) 3560
BssSI CACGAG 1 cut(s) 3496
BssT1I CCWWGG 4 cut(s) 775, 1223, 3229, 3609
Bst2BI CACGAG 1 cut(s) 3496
Bst2UI CCWGG 7 cut(s) 443, 585, 788, 1853, 2928, 3106, 3577
Bst4CI ACNGT 9 cut(s) 189, 331, 742, 830, 1253, 1885, 2759, 2900, 3195
Bst6I CTCTTC 6 cut(s) 287, 522, 1197, 2151, 2256, 2327
BstAPI GCANNNNNTGC 1 cut(s) 2107
BstBAI YACGTR 1 cut(s) 2185
BstBI TTCGAA 4 cut(s) 47, 1360, 1972, 3644
BstC8I GCNNGC 2 cut(s) 1142, 2985
BstDSI CCRYGG 1 cut(s) 3609
BstENI CCTNNNNNAGG 1 cut(s) 3528
BstFNI CGCG 1 cut(s) 803
BstHHI GCGC 2 cut(s) 1881, 2112
BstMAI GTCTC 5 cut(s) 359, 988, 2258, 2945, 3207
BstMCI CGRYCG 1 cut(s) 2761
BstMWI GCNNNNNNNGC 8 cut(s) 283, 331, 1601, 2030, 2107, 2426, 3010, 3561
BstNI CCWGG 7 cut(s) 443, 585, 788, 1853, 2928, 3106, 3577
BstNSI RCATGY 5 cut(s) 200, 1035, 2710, 2958, 3550
BstSFI CTRYAG 4 cut(s) 741, 1468, 1800, 2685
BstSLI GKGCMC 5 cut(s) 336, 1780, 3207, 3373, 3583
BstSNI TACGTA 1 cut(s) 2185
BstUI CGCG 1 cut(s) 803
BstV1I GCAGC 8 cut(s) 1037, 1510, 1808, 1888, 2160, 2432, 3472, 3567
BstV2I GAAGAC 4 cut(s) 636, 895, 1203, 1373
BstX2I RGATCY 3 cut(s) 2585, 3262, 3515
BstXI CCANNNNNNTGG 2 cut(s) 108, 1230
BstYI RGATCY 3 cut(s) 2585, 3262, 3515
Bsu36I CCTNAGG 2 cut(s) 722, 2037
BsuI GTATCC 2 cut(s) 3217, 3527
BsuRI GGCC 3 cut(s) 427, 1476, 3589
BtgI CCRYGG 1 cut(s) 3609
BtrI CACGTC 2 cut(s) 2118, 3450
BtsI GCAGTG 3 cut(s) 162, 334, 3118
BveI ACCTGC 2 cut(s) 3532, 3650
Cac8I GCNNGC 2 cut(s) 1142, 2985
CaiI CAGNNNCTG 3 cut(s) 187, 896, 2557
CfoI GCGC 2 cut(s) 1881, 2112
Cfr10I RCCGGY 1 cut(s) 3560
Cfr13I GGNCC 9 cut(s) 1312, 1347, 1474, 3147, 3155, 3326, 3416, 3588, 3626
Cfr9I CCCGGG 1 cut(s) 2581
CseI GACGC 1 cut(s) 809
CsiI ACCWGGT 1 cut(s) 441
Csp6I GTAC 7 cut(s) 1215, 1256, 2007, 2182, 2351, 3040, 3287
CviQI GTAC 7 cut(s) 1215, 1256, 2007, 2182, 2351, 3040, 3287
DraIII CACNNNGTG 1 cut(s) 349
DrdI GACNNNNNNGTC 2 cut(s) 980, 3427
DseDI GACNNNNNNGTC 2 cut(s) 980, 3427
Eam1104I CTCTTC 6 cut(s) 287, 522, 1197, 2151, 2256, 2327
EarI CTCTTC 6 cut(s) 287, 522, 1197, 2151, 2256, 2327
EciI GGCGGA 1 cut(s) 3442
Eco105I TACGTA 1 cut(s) 2185
Eco130I CCWWGG 4 cut(s) 775, 1223, 3229, 3609
Eco147I AGGCCT 1 cut(s) 427
Eco32I GATATC 2 cut(s) 243, 1494
Eco47I GGWCC 7 cut(s) 1312, 1347, 3147, 3155, 3326, 3416, 3626
Eco57I CTGAAG 8 cut(s) 164, 263, 648, 873, 1011, 1479, 1529, 2031
Eco81I CCTNAGG 2 cut(s) 722, 2037
Eco88I CYCGRG 1 cut(s) 2581
EcoNI CCTNNNNNAGG 1 cut(s) 3528
EcoRI GAATTC 1 cut(s) 1463
EcoRII CCWGG 7 cut(s) 441, 583, 786, 1851, 2926, 3104, 3575
EcoRV GATATC 2 cut(s) 243, 1494
EcoT14I CCWWGG 4 cut(s) 775, 1223, 3229, 3609
EcoT22I ATGCAT 1 cut(s) 493
ErhI CCWWGG 4 cut(s) 775, 1223, 3229, 3609
Esp3I CGTCTC 1 cut(s) 2258
FalI AAGNNNNNCTT 4 cut(s) 1124, 1156, 2264, 2296
FaqI GGGAC 5 cut(s) 1101, 1325, 2820, 3073, 3141
FauNDI CATATG 1 cut(s) 2527
FbaI TGATCA 3 cut(s) 1660, 2385, 2779
FblI GTMKAC 1 cut(s) 798
FspBI CTAG 4 cut(s) 869, 1991, 3015, 3347
GlaI GCGC 2 cut(s) 1880, 2111
GsuI CTGGAG 2 cut(s) 1019, 2671
HaeIII GGCC 3 cut(s) 427, 1476, 3589
HapII CCGG 7 cut(s) 372, 1145, 2582, 3561, 3586, 3591, 3624
HgaI GACGC 1 cut(s) 809
HhaI GCGC 2 cut(s) 1881, 2112
Hin6I GCGC 2 cut(s) 1879, 2110
HinP1I GCGC 2 cut(s) 1879, 2110
HincII GTYRAC 3 cut(s) 517, 3070, 3596
HindII GTYRAC 3 cut(s) 517, 3070, 3596
HindIII AAGCTT 3 cut(s) 275, 2255, 3097
HpaI GTTAAC 1 cut(s) 3070
HpaII CCGG 7 cut(s) 372, 1145, 2582, 3561, 3586, 3591, 3624
HpyCH4III ACNGT 9 cut(s) 189, 331, 742, 830, 1253, 1885, 2759, 2900, 3195
HpyCH4IV ACGT 7 cut(s) 946, 1766, 2117, 2184, 3285, 3289, 3449
HpyF10VI GCNNNNNNNGC 8 cut(s) 283, 331, 1601, 2030, 2107, 2426, 3010, 3561
HpySE526I ACGT 7 cut(s) 946, 1766, 2117, 2184, 3285, 3289, 3449
HspAI GCGC 2 cut(s) 1879, 2110
Ksp22I TGATCA 3 cut(s) 1660, 2385, 2779
KspAI GTTAAC 1 cut(s) 3070
LmnI GCTCC 7 cut(s) 878, 1489, 2038, 2475, 2891, 3108, 3625
Lsp1109I GCAGC 8 cut(s) 1037, 1510, 1808, 1888, 2160, 2432, 3472, 3567
MabI ACCWGGT 1 cut(s) 441
MaeI CTAG 4 cut(s) 869, 1991, 3015, 3347
MaeII ACGT 7 cut(s) 946, 1766, 2117, 2184, 3285, 3289, 3449
MfeI CAATTG 1 cut(s) 2381
MflI RGATCY 3 cut(s) 2585, 3262, 3515
MhlI GDGCHC 7 cut(s) 252, 336, 1780, 2035, 3207, 3373, 3583
MlyI GAGTC 9 cut(s) 644, 990, 2662, 2738, 2932, 3130, 3139, 3287, 3437
MmeI TCCRAC 3 cut(s) 1315, 3443, 3459
Mph1103I ATGCAT 1 cut(s) 493
MroXI GAANNNNTTC 5 cut(s) 296, 815, 1410, 1450, 2318
MseI TTAA 9 cut(s) 477, 1287, 1769, 1823, 2300, 2415, 2507, 3069, 3332
MslI CAYNNNNRTG 3 cut(s) 152, 2243, 3500
MspA1I CMGCKG 3 cut(s) 1457, 3152, 3463
MspI CCGG 7 cut(s) 372, 1145, 2582, 3561, 3586, 3591, 3624
MunI CAATTG 1 cut(s) 2381
Mva1269I GAATGC 2 cut(s) 2112, 2737
MvaI CCWGG 7 cut(s) 443, 585, 788, 1853, 2928, 3106, 3577
MvnI CGCG 1 cut(s) 803
MwoI GCNNNNNNNGC 8 cut(s) 283, 331, 1601, 2030, 2107, 2426, 3010, 3561
NciI CCSGG 5 cut(s) 2582, 2583, 3586, 3591, 3624
NcoI CCATGG 1 cut(s) 3609
NdeI CATATG 1 cut(s) 2527
NlaIV GGNNCC 9 cut(s) 33, 874, 1313, 1475, 2224, 3157, 3192, 3582, 3607
NmeAIII GCCGAG 2 cut(s) 312, 3451
NmuCI GTSAC 5 cut(s) 641, 2376, 2702, 2863, 2903
NsiI ATGCAT 1 cut(s) 493
NspI RCATGY 5 cut(s) 200, 1035, 2710, 2958, 3550
NspV TTCGAA 4 cut(s) 47, 1360, 1972, 3644
PasI CCCWGGG 1 cut(s) 3576
PceI AGGCCT 1 cut(s) 427
PciI ACATGT 3 cut(s) 1031, 2706, 3546
PctI GAATGC 2 cut(s) 2112, 2737
PdmI GAANNNNTTC 5 cut(s) 296, 815, 1410, 1450, 2318
Pfl23II CGTACG 1 cut(s) 3286
PfoI TCCNGGA 1 cut(s) 3104
PleI GAGTC 9 cut(s) 643, 989, 2661, 2738, 2931, 3130, 3138, 3286, 3436
PpsI GAGTC 9 cut(s) 643, 989, 2661, 2738, 2931, 3130, 3138, 3286, 3436
Ppu21I YACGTR 1 cut(s) 2185
PscI ACATGT 3 cut(s) 1031, 2706, 3546
PsiI TTATAA 3 cut(s) 969, 1865, 2286
Psp1406I AACGTT 1 cut(s) 1766
Psp6I CCWGG 7 cut(s) 441, 583, 786, 1851, 2926, 3104, 3575
PspGI CCWGG 7 cut(s) 441, 583, 786, 1851, 2926, 3104, 3575
PspLI CGTACG 1 cut(s) 3286
PspN4I GGNNCC 9 cut(s) 33, 874, 1313, 1475, 2224, 3157, 3192, 3582, 3607
PspPI GGNCC 9 cut(s) 1312, 1347, 1474, 3147, 3155, 3326, 3416, 3588, 3626
PstNI CAGNNNCTG 3 cut(s) 187, 896, 2557
PsuI RGATCY 3 cut(s) 2585, 3262, 3515
RsaI GTAC 7 cut(s) 1216, 1257, 2008, 2183, 2352, 3041, 3288
RsaNI GTAC 7 cut(s) 1215, 1256, 2007, 2182, 2351, 3040, 3287
RseI CAYNNNNRTG 3 cut(s) 152, 2243, 3500
SaqAI TTAA 9 cut(s) 477, 1287, 1769, 1823, 2300, 2415, 2507, 3069, 3332
Sau96I GGNCC 9 cut(s) 1312, 1347, 1474, 3147, 3155, 3326, 3416, 3588, 3626
ScaI AGTACT 1 cut(s) 2008
SchI GAGTC 9 cut(s) 644, 990, 2662, 2738, 2932, 3130, 3139, 3287, 3437
SduI GDGCHC 7 cut(s) 252, 336, 1780, 2035, 3207, 3373, 3583
SexAI ACCWGGT 1 cut(s) 441
SfcI CTRYAG 4 cut(s) 741, 1468, 1800, 2685
SfuI TTCGAA 4 cut(s) 47, 1360, 1972, 3644
SinI GGWCC 7 cut(s) 1312, 1347, 3147, 3155, 3326, 3416, 3626
SmaI CCCGGG 1 cut(s) 2583
SmiMI CAYNNNNRTG 3 cut(s) 152, 2243, 3500
SmlI CTYRAG 4 cut(s) 272, 2453, 2691, 3127
SmoI CTYRAG 4 cut(s) 272, 2453, 2691, 3127
SnaBI TACGTA 1 cut(s) 2185
SseBI AGGCCT 1 cut(s) 427
SspMI CTAG 4 cut(s) 869, 1991, 3015, 3347
StuI AGGCCT 1 cut(s) 427
StyI CCWWGG 4 cut(s) 775, 1223, 3229, 3609
TaaI ACNGT 9 cut(s) 189, 331, 742, 830, 1253, 1885, 2759, 2900, 3195
TaiI ACGT 7 cut(s) 949, 1769, 2120, 2187, 3288, 3292, 3452
TaqI TCGA 8 cut(s) 47, 231, 300, 1360, 1972, 2442, 2460, 3644
TaqII GACCGA 1 cut(s) 2751
TatI WGTACW 3 cut(s) 1255, 2006, 2350
TauI GCSGC 3 cut(s) 1838, 1876, 2635
Tru1I TTAA 9 cut(s) 477, 1287, 1769, 1823, 2300, 2415, 2507, 3069, 3332
Tru9I TTAA 9 cut(s) 477, 1287, 1769, 1823, 2300, 2415, 2507, 3069, 3332
TseFI GTSAC 5 cut(s) 641, 2376, 2702, 2863, 2903
TseI GCWGC 8 cut(s) 1050, 1523, 1796, 1876, 2173, 2420, 3460, 3555
Tsp45I GTSAC 5 cut(s) 641, 2376, 2702, 2863, 2903
TspGWI ACGGA 5 cut(s) 31, 1411, 1999, 2118, 3057
TspMI CCCGGG 1 cut(s) 2581
VneI GTGCAC 3 cut(s) 332, 1776, 3369
VpaK11BI GGWCC 7 cut(s) 1312, 1347, 3147, 3155, 3326, 3416, 3626
XagI CCTNNNNNAGG 1 cut(s) 3528
XapI RAATTY 3 cut(s) 1463, 2964, 3652
XceI RCATGY 5 cut(s) 200, 1035, 2710, 2958, 3550
XcmI CCANNNNNNNNNTGG 4 cut(s) 1918, 2558, 2646, 3410
XmaI CCCGGG 1 cut(s) 2581
XmiI GTMKAC 1 cut(s) 798
XmnI GAANNNNTTC 5 cut(s) 296, 815, 1410, 1450, 2318
XspI CTAG 4 cut(s) 869, 1991, 3015, 3347
ZrmI AGTACT 1 cut(s) 2008
Zsp2I ATGCAT 1 cut(s) 493
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.