Rh4DG123700

metal ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0000343
Physical Location & Seq
Forward (+)
19940048 .. 19945361
5314 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG123700.1

Sequence Viewer

Length: 2850 bp
ATGGGTTCTTCTGTGAATTTGGTCAGGTGTAAGTTAAACAAGGGTATGGCATTGGCAGAGTGTCAACCATATTCTGTTTCGGAGTACCATTTCGTAGATGACAAGGATGAATGTGTCTCATTTCATGTATTGCCTGTTCAATGGAGTGATGGTGAGAGACTGGATGGTGAGAAAGTAAAGATTTTTCTGCATGGAACCGCTGATAATGGGGGGAATTTATGTAAGCATGTTGTTGCATGGAAGTTTGACATTTCGAGTGAAGAGCCCGAGATATTGGTTCTCCTCTCCGAGGAAAATAGTTGGATCAAGCTTGGGAAGCCGAGGAAGAGCTTTGAGGACACTGTTAGGTCGATCTTGATAACGGTGCACTGCCTTCACTATGTGAAGAGAAATCCTGAAAAATCCAGAGAATGTGTGTGGAATCACCTATCTGAAGTTTTCAGGTTGTATGTGGTCAGGCCTTCTCAAAGTGATCTGGTGGATCACATGCCTCTAATCGGTGATTGTGTTAACAGATATCGTGCCCTAGCGGATTCCAAGTTTTTGGCCAAACTTTTTGAAGAGAAGAGGAAACTCTCTGATGAGGATACTGAAGCTAGAAAGAAGCTCAGAATTATGATCGATGATGTAAAAGATGATACGATTCATGCAGAAGATGAGGATATCGATAACCTGTTTGGTGAGGTCTGTGCTTTATGTGACGATGGTGGCAAGATCATTTGTTGTGAAGGGGGGTGCTTAAGGTCTTTTCATGCAACTGAGGAGGCGGGTGCTGGATCTGATTGCAAATCTCTTGGCTTTATGCAGGATGATGTTGTTGGGATGCAAAATTTCTACTGCAAGAACTGTGAATATAAACAGCACCAGTGCTTTGTTTGTGGGAAGTTAGGTTCCTCTGATAAGTCCTCAGGTGCTGCTGAGGTGGTTTCCTGTGTATCCCCAAACTGTGGTCGATTTTACCATCCACATTGTGTTGCAATATTACTTTATCAAGATAATGGAGTTCCTGCTGAAGAACTTGAGAGAAAGATTGCTGGTGGGGAATCTTTTACTTGTCCACTTCATAAGTGCTGCATTTGCAAACAAGGAGAAAATAAGAAGGATAGTGAGTTACAATTTGCTGTGTGCATGCGTTGTCCAAAATCATACCACAGGAAATGCCTGCCAAGTGAGATTGCTTTTGAAAATCAAGGAGAAATGCTAGAAGGGAGATCAATAATAAGAGCTTGGGATGGTCTATTACCTAACCGTATACTCATATATTGCACAAAACATGATATAGATATTGAATTTGGAACTGTAAGAAGGGACCACGTAAAATTCCCTGATGTCAAACAGAATATGAGCACTCTCAAAAAGAAGAAGAAGACTATTTTAGAAGAGAAGTGGAAGCCAATATCAGAATTCCTTGAAGACGGAGACAAAGTTGGGACTAAGAGAAATATTTCCTTGGAAAAATCATGTAGGGATAAAAATGCTCCTACAGCACTTAAGACACAAAAGCCATATGTTCAAGAGAAGAAGACTAGGAAACTTATGTCAGAGCAGAAACCTGTGTCTAAGAAGAGCAATTCTTCTTTGGAAGAATCAGTTGGGGAAGGAAGTGATCCTGTACTATCCACACTAGTCAAGCAGAAGCTTTATTCAGTTAAAGAGAAGAAGGCTACTTTTGTAAAAGAGAAAACTTTTGTTGAAGAGAAAGAAAGCCAACTGACATCAACGGTCTCTGTAGACAGAGAAAAAGCTGTGTTGAAGAGGAGAACTCCTTCCTCTGAAGGACTATATAGTGAGAAAAGTGCTCCTACAATATCCAAGAAAAAACAGAAGCCATCTTTTGTTGAAGAGAAGAACAATGACCTTGTAAAAAAGAAAACTAATGTTGAAGAGAAGAGGAAACAGACATCAGAGTCCCTTGTTGAGAGAGAGACAGCTGTGTCCAAAAAGAAAAATCCTTCCTCGAAAGAATCCTATAGGGAAAGAAATGCTTCTACAACTCCCAAACAGAAGCAGAAGCGATCAGAGCTCACTGTAGATGGAGGTGAGGCTGGGTCTAAGAAGGGAAGTCCTTTGGAAGAATCATTAAGTGCAATTGCTTCAACTGTACCCAAACTGAAGCAGAAGTCGACTTCTGCCATGAAACTGGGTAGTAACAAGAATAGGGAAAAGACGTTTTCTGGTATAGATATTTCAAGGATGGTGAAACGAAATAATTCACTGAAAAATGAACATCAGGCTTCCATGTCTGAGAAAATCAAGACCTCACTGGGTGAAGAGCCATTTTGTTCAATGGAATCTGAGCAGGTGAAGCTCGGGAACCTGGATACTCCTGATGCTAATACAGTTAGTGAGATACACAGGGAAATGCCACCACATACTGAATGTGGTGACGATGGAAATCAGCATCCTAACCCCAGCAAGTCTCGTATGCACTGTGCTCATGCTAACAGTCGAGTCCAATTCAAAATTCCTGATGATGCAGGCAGAAGGTGCACCACGAGTAATGATGAGCCTCGTTCGAGTGTTACCAATCCTAGGTCTGGCAATGCGAAAGGGTGCAAGAGAGAGAACTCGGGAAAACCATATTCAAATGGGACGAAACAGAAACAGAAACTGAGAACAGAATTTGACAGAGTCCCACCGGCTTGCATTAAAGGGAAGCAAGATCCTATTTTTTCAAGAGGCAATGATCTAGCGGGCCAAAATTCAGTATTAGCTATGCCATCAGATGCAACTAAATTGGTTGGTCCAAGAGCACCCCAACCTGATTACCTGGATAGGTGTATGGGTTTCGCTCCTGGTCTGCACCTGAACTATGCACTACAGAATTCAGCTGGTTGGATCGATGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006325 GO:0006355 GO:0006950 GO:0006952 GO:0006996 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0009605 GO:0009607 GO:0009620 GO:0009791 GO:0009889 GO:0009893 GO:0009909 GO:0009911 GO:0009987 GO:0010228 GO:0010468 GO:0010556 GO:0010604 GO:0010638 GO:0016043 GO:0019219 GO:0019222 GO:0022414 GO:0023052 GO:0030154 GO:0031056 GO:0031058 GO:0031060 GO:0031062 GO:0031323 GO:0031325 GO:0031326 GO:0031399 GO:0031401 GO:0031440 GO:0031935 GO:0031937 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033043 GO:0033044 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0043954 GO:0044030 GO:0044424 GO:0044464 GO:0048366 GO:0048367 GO:0048468 GO:0048518 GO:0048522 GO:0048580 GO:0048582 GO:0048608 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0048869 GO:0050684 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051052 GO:0051094 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051276 GO:0051570 GO:0051574 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060968 GO:0061085 GO:0061087 GO:0061458 GO:0065007 GO:0070827 GO:0070828 GO:0070829 GO:0071840 GO:0080090 GO:0090436 GO:0097159 GO:0098542 GO:0099402 GO:0140110 GO:1900109 GO:1900111 GO:1900363 GO:1901363 GO:1902275 GO:1903311 GO:1903506 GO:1905269 GO:2000024 GO:2000026 GO:2000112 GO:2000241 GO:2000243 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNMT1-RFD

Protein Analysis

949

Amino Acids

106.42

Weight (kDa)

8.67

Isoelectric Point (pI)

49.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNMT1-RFD PF12047 26 - 148 7.7e-10 Cytosine specific DNA methyltransferase replication foci domain
PHD_NSD PF22908 286 - 355 3.3e-19 Histone-lysine N-methyltransferase NSD-like, PHD zinc finger
PHDvar_NSD PF23004 357 - 393 7.9e-06 Histone-lysine N-methyltransferase NSD-like, variant PHD zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000343)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48090 AT5G48090 AT5G48090 AT5G55390 AT5G55390 AT5G55390
fragaria_vesca FvH4_3g23690 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160
malus_domestica MD03G1191000.v1.1 MD11G1207800.v1.1 MD11G1208000.v1.1
prunus_persica Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227600_v2.0.a1 Prupe.4G227700_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1
pyrus_communis pycom01g03400 pycom03g14420 pycom11g18100 pycom11g18140 pycom11g18150 pycom11g18160
rosa_chinensis RchiOBHm_Chr2g0145981 RchiOBHm_Chr4g0405241 RchiOBHm_Chr4g0405271 RchiOBHm_Chr5g0016201 RchiOBHm_Chr5g0041831 RchiOBHm_Chr5g0041871 RchiOBHm_Chr5g0041881 RchiOBHm_Chr5g0059241
rosa_laevigata RLG00000002117 RLG00000008861 RLG00000008862 RLG00000011000 RLG00000033216 RLG00000034097 RLG00000034101
rosa_multiflora Rmu_sc0000161.1_g000001 Rmu_sc0000861.1_g000040 Rmu_sc0007522.1_g000003 Rmu_sc0009443.1_g000015 Rmu_sc0022129.1_g000001 Rmu_sc0042315.1_g000001
rosa_roxburghii Rroxscaffold_1G00038660 Rroxscaffold_1G00038690 Rroxscaffold_5G00349560 Rroxscaffold_5G00349610 Rroxscaffold_6G00388370 Rroxscaffold_7G00188740
rosa_rugosa Rorug03G0199700 Rorug04G0055400 Rorug04G0055500 Rorug04G0055900 Rorug04G0056000 Rorug04G0056100 Rorug04G0056100 Rorug04G0056200 Rorug05G0195600 Rorug05G0195700 Rorug05G0195800 Rorug05G0195900 Rorug05G0196800 Rorug05G0385000
rosa_samantha Rh4AG129900 Rh4AG130100 Rh4BG124200 Rh4BG124400 Rh4BG124500 Rh4CG137200 Rh4CG137300 Rh4DG123500 Rh4DG123700 Rh5AG280800 Rh5AG281400 Rh5BG286700 Rh5BG287000 Rh5BG397800 Rh5CG318100 Rh5CG318600 Rh5CG421600 Rh5DG132300 Rh5DG132400 Rh5DG295400 Rh5DG295700 Rh7BG475300
rosa_wichuraiana Rw0G001330 Rw0G005730 Rw4G008670 Rw4G010510 Rw4G010540 Rw5G026450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2290
AasI GACNNNNNNGTC 2 cut(s) 1906, 1933
Acc36I ACCTGC 1 cut(s) 2290
AccB7I CCANNNNNTGG 1 cut(s) 947
AccI GTMKAC 3 cut(s) 1252, 1731, 2123
AciI CCGC 4 cut(s) 198, 530, 767, 2693
AclWI GGATC 6 cut(s) 311, 489, 784, 1601, 2657, 2846
AcoI YGGCCR 1 cut(s) 546
AcuI CTGAAG 5 cut(s) 453, 612, 1032, 1794, 2132
AdeI CACNNNGTG 3 cut(s) 382, 971, 2267
AfaI GTAC 3 cut(s) 86, 1614, 2103
AfiI CCNNNNNNNGG 5 cut(s) 289, 497, 947, 2532, 2537
AflII CTTAAG 2 cut(s) 739, 1490
AhlI ACTAGT 1 cut(s) 1624
AjnI CCWGG 3 cut(s) 2316, 2769, 2794
AjuI GAANNNNNNNTTGG 4 cut(s) 1575, 1607, 2448, 2480
AloI GAACNNNNNNTCC 2 cut(s) 874, 906
Alw21I GWGCWC 7 cut(s) 369, 1349, 1801, 2025, 2437, 2492, 2755
Alw26I GTCTC 6 cut(s) 121, 151, 1413, 1729, 1919, 2424
Alw44I GTGCAC 2 cut(s) 365, 2488
AlwI GGATC 6 cut(s) 311, 489, 784, 1601, 2657, 2846
AlwNI CAGNNNCTG 2 cut(s) 914, 2611
Ama87I CYCGRG 3 cut(s) 266, 2309, 2569
AoxI GGCC 3 cut(s) 458, 546, 2695
ApaLI GTGCAC 2 cut(s) 365, 2488
ApeKI GCWGC 2 cut(s) 914, 1071
Asp700I GAANNNNTTC 4 cut(s) 1444, 1765, 1984, 2209
AspA2I CCTAGG 1 cut(s) 2531
AspS9I GGNCC 3 cut(s) 1309, 2695, 2744
AvaI CYCGRG 3 cut(s) 266, 2309, 2569
AvaII GGWCC 2 cut(s) 1309, 2744
AvrII CCTAGG 1 cut(s) 2531
AxyI CCTNAGG 1 cut(s) 907
BaeGI GKGCMC 3 cut(s) 369, 526, 2492
BalI TGGCCA 1 cut(s) 548
BanII GRGCYC 2 cut(s) 267, 2025
BauI CACGAG 1 cut(s) 2494
BbsI GAAGAC 3 cut(s) 1373, 1419, 1529
Bbv12I GWGCWC 7 cut(s) 369, 1349, 1801, 2025, 2437, 2492, 2755
BbvCI CCTCAGC 1 cut(s) 918
BbvI GCAGC 2 cut(s) 901, 1058
BciT130I CCWGG 3 cut(s) 2318, 2771, 2796
BciVI GTATCC 3 cut(s) 580, 946, 2314
BcoDI GTCTC 6 cut(s) 121, 151, 1413, 1729, 1919, 2424
BcuI ACTAGT 1 cut(s) 1624
BfaI CTAG 7 cut(s) 527, 597, 1202, 1527, 1625, 2532, 2690
BfmI CTRYAG 5 cut(s) 1482, 1728, 1969, 2028, 2819
BfrI CTTAAG 2 cut(s) 739, 1490
BfuAI ACCTGC 1 cut(s) 2290
BfuI GTATCC 3 cut(s) 580, 946, 2314
BisI GCNGC 2 cut(s) 915, 1072
BlnI CCTAGG 1 cut(s) 2531
BlsI GCNGC 2 cut(s) 916, 1073
Bme1390I CCNGG 3 cut(s) 2318, 2771, 2796
Bme18I GGWCC 2 cut(s) 1309, 2744
BmeT110I CYCGRG 3 cut(s) 266, 2309, 2569
BmgT120I GGNCC 3 cut(s) 1309, 2695, 2744
BmiI GGNNCC 4 cut(s) 196, 892, 1310, 2315
BmrFI CCNGG 3 cut(s) 2318, 2771, 2796
BmrI ACTGGG 2 cut(s) 2150, 2273
BmsI GCATC 5 cut(s) 813, 2320, 2410, 2464, 2716
BmuI ACTGGG 2 cut(s) 2150, 2273
BpiI GAAGAC 3 cut(s) 1373, 1419, 1529
BplI GAGNNNNNCTC 2 cut(s) 1747, 1779
Bpu10I CCTNAGC 1 cut(s) 918
BpuEI CTTGAG 1 cut(s) 1040
Bsa29I ATCGAT 3 cut(s) 621, 666, 2841
BsaAI YACGTR 1 cut(s) 1315
BsaBI GATNNNNATC 1 cut(s) 2394
BsaI GGTCTC 1 cut(s) 1729
BsaJI CCNNGG 4 cut(s) 288, 320, 1449, 2531
BsaXI ACNNNNNCTCC 4 cut(s) 136, 166, 1410, 1440
Bsc4I CCNNNNNNNGG 5 cut(s) 289, 497, 947, 2532, 2537
Bse118I RCCGGY 1 cut(s) 2638
Bse1I ACTGG 4 cut(s) 165, 865, 2145, 2268
Bse21I CCTNAGG 1 cut(s) 907
Bse3DI GCAATG 2 cut(s) 2548, 2689
Bse8I GATNNNNATC 1 cut(s) 2394
BseBI CCWGG 3 cut(s) 2318, 2771, 2796
BseCI ATCGAT 3 cut(s) 621, 666, 2841
BseDI CCNNGG 4 cut(s) 288, 320, 1449, 2531
BseGI GGATG 8 cut(s) 112, 169, 814, 828, 961, 1237, 2199, 2401
BseJI GATNNNNATC 1 cut(s) 2394
BseLI CCNNNNNNNGG 5 cut(s) 289, 497, 947, 2532, 2537
BseMI GCAATG 2 cut(s) 2548, 2689
BseMII CTCAG 7 cut(s) 622, 750, 909, 921, 2235, 2286, 2603
BseNI ACTGG 4 cut(s) 165, 865, 2145, 2268
BseRI GAGGAG 3 cut(s) 272, 776, 1771
BseSI GKGCMC 3 cut(s) 369, 526, 2492
BseXI GCAGC 2 cut(s) 901, 1058
BseYI CCCAGC 2 cut(s) 2045, 2411
BsgI GTGCAG 1 cut(s) 2786
BshFI GGCC 3 cut(s) 460, 548, 2697
BshVI ATCGAT 3 cut(s) 621, 666, 2841
BsiHKAI GWGCWC 7 cut(s) 369, 1349, 1801, 2025, 2437, 2492, 2755
BsiHKCI CYCGRG 3 cut(s) 266, 2309, 2569
BsiSI CCGG 1 cut(s) 2639
BslFI GGGAC 5 cut(s) 1322, 1444, 1894, 2605, 2618
BslI CCNNNNNNNGG 5 cut(s) 289, 497, 947, 2532, 2537
BsmAI GTCTC 6 cut(s) 121, 151, 1413, 1729, 1919, 2424
BsmFI GGGAC 5 cut(s) 1322, 1444, 1894, 2605, 2618
BsnI GGCC 3 cut(s) 460, 548, 2697
Bso31I GGTCTC 1 cut(s) 1729
BsoBI CYCGRG 3 cut(s) 266, 2309, 2569
Bsp1286I GDGCHC 9 cut(s) 267, 369, 526, 1349, 1801, 2025, 2437, 2492, 2755
BspACI CCGC 4 cut(s) 198, 530, 767, 2693
BspANI GGCC 3 cut(s) 460, 548, 2697
BspCNI CTCAG 7 cut(s) 621, 751, 910, 920, 2236, 2287, 2604
BspDI ATCGAT 3 cut(s) 621, 666, 2841
BspLI GGNNCC 4 cut(s) 196, 892, 1310, 2315
BspMI ACCTGC 1 cut(s) 2290
BspPI GGATC 6 cut(s) 311, 489, 784, 1601, 2657, 2846
BspQI GCTCTTC 4 cut(s) 255, 320, 1559, 2265
BspTI CTTAAG 2 cut(s) 739, 1490
BspTNI GGTCTC 1 cut(s) 1729
BsrDI GCAATG 2 cut(s) 2548, 2689
BsrFI RCCGGY 1 cut(s) 2638
BsrI ACTGG 4 cut(s) 165, 865, 2145, 2268
BssAI RCCGGY 1 cut(s) 2638
BssECI CCNNGG 4 cut(s) 288, 320, 1449, 2531
BssNAI GTATAC 1 cut(s) 1253
BssSI CACGAG 1 cut(s) 2494
BssT1I CCWWGG 2 cut(s) 1449, 2531
Bst1107I GTATAC 1 cut(s) 1253
Bst2BI CACGAG 1 cut(s) 2494
Bst2UI CCWGG 3 cut(s) 2318, 2771, 2796
BstAFI CTTAAG 2 cut(s) 739, 1490
BstAPI GCANNNNNTGC 1 cut(s) 2487
BstBAI YACGTR 1 cut(s) 1315
BstC8I GCNNGC 5 cut(s) 1130, 1163, 2479, 2644, 2695
BstENI CCTNNNNNAGG 1 cut(s) 287
BstF5I GGATG 8 cut(s) 112, 169, 814, 828, 961, 1237, 2199, 2401
BstMAI GTCTC 6 cut(s) 121, 151, 1413, 1729, 1919, 2424
BstMWI GCNNNNNNNGC 6 cut(s) 316, 1077, 1484, 2020, 2305, 2487
BstNI CCWGG 3 cut(s) 2318, 2771, 2796
BstNSI RCATGY 3 cut(s) 230, 490, 1132
BstSCI CCNGG 3 cut(s) 2316, 2769, 2794
BstSFI CTRYAG 5 cut(s) 1482, 1728, 1969, 2028, 2819
BstSLI GKGCMC 3 cut(s) 369, 526, 2492
BstV1I GCAGC 2 cut(s) 901, 1058
BstV2I GAAGAC 3 cut(s) 1373, 1419, 1529
BstX2I RGATCY 2 cut(s) 776, 2662
BstXI CCANNNNNNTGG 2 cut(s) 544, 2140
BstYI RGATCY 2 cut(s) 776, 2662
BstZ17I GTATAC 1 cut(s) 1253
Bsu15I ATCGAT 3 cut(s) 621, 666, 2841
Bsu36I CCTNAGG 1 cut(s) 907
BsuI GTATCC 3 cut(s) 580, 946, 2314
BsuRI GGCC 3 cut(s) 460, 548, 2697
BsuTUI ATCGAT 3 cut(s) 621, 666, 2841
BtsCI GGATG 8 cut(s) 112, 169, 814, 828, 961, 1237, 2199, 2401
BtsI GCAGTG 1 cut(s) 367
BtsIMutI CAGTG 7 cut(s) 339, 367, 872, 2025, 2213, 2261, 2428
BveI ACCTGC 1 cut(s) 2290
Cac8I GCNNGC 5 cut(s) 1130, 1163, 2479, 2644, 2695
CaiI CAGNNNCTG 2 cut(s) 914, 2611
Cfr10I RCCGGY 1 cut(s) 2638
Cfr13I GGNCC 3 cut(s) 1309, 2695, 2744
ClaI ATCGAT 3 cut(s) 621, 666, 2841
Csp6I GTAC 3 cut(s) 85, 1613, 2102
CviQI GTAC 3 cut(s) 85, 1613, 2102
DraIII CACNNNGTG 3 cut(s) 382, 971, 2267
DrdI GACNNNNNNGTC 2 cut(s) 1906, 1933
DseDI GACNNNNNNGTC 2 cut(s) 1906, 1933
EaeI YGGCCR 1 cut(s) 546
Ecl136II GAGCTC 1 cut(s) 2023
Eco130I CCWWGG 2 cut(s) 1449, 2531
Eco147I AGGCCT 1 cut(s) 460
Eco24I GRGCYC 2 cut(s) 267, 2025
Eco31I GGTCTC 1 cut(s) 1729
Eco32I GATATC 2 cut(s) 518, 664
Eco47I GGWCC 2 cut(s) 1309, 2744
Eco53kI GAGCTC 1 cut(s) 2023
Eco57I CTGAAG 5 cut(s) 453, 612, 1032, 1794, 2132
Eco81I CCTNAGG 1 cut(s) 907
Eco88I CYCGRG 3 cut(s) 266, 2309, 2569
EcoICRI GAGCTC 1 cut(s) 2023
EcoNI CCTNNNNNAGG 1 cut(s) 287
EcoRI GAATTC 2 cut(s) 1403, 2824
EcoRII CCWGG 3 cut(s) 2316, 2769, 2794
EcoRV GATATC 2 cut(s) 518, 664
EcoT14I CCWWGG 2 cut(s) 1449, 2531
EcoT38I GRGCYC 2 cut(s) 267, 2025
ErhI CCWWGG 2 cut(s) 1449, 2531
FalI AAGNNNNNCTT 2 cut(s) 1969, 2001
FaqI GGGAC 5 cut(s) 1322, 1444, 1894, 2605, 2618
FauI CCCGC 2 cut(s) 760, 2686
FauNDI CATATG 1 cut(s) 1507
FblI GTMKAC 3 cut(s) 1252, 1731, 2123
Fnu4HI GCNGC 2 cut(s) 915, 1072
FokI GGATG 8 cut(s) 119, 176, 821, 835, 948, 1244, 2206, 2388
FriOI GRGCYC 2 cut(s) 267, 2025
Fsp4HI GCNGC 2 cut(s) 915, 1072
FspBI CTAG 7 cut(s) 527, 597, 1202, 1527, 1625, 2532, 2690
GluI GCNGC 2 cut(s) 915, 1072
GsaI CCCAGC 2 cut(s) 2049, 2415
HaeIII GGCC 3 cut(s) 460, 548, 2697
HapII CCGG 1 cut(s) 2639
HincII GTYRAC 3 cut(s) 65, 511, 2124
HindII GTYRAC 3 cut(s) 65, 511, 2124
HindIII AAGCTT 2 cut(s) 308, 1637
HpaI GTTAAC 1 cut(s) 511
HpaII CCGG 1 cut(s) 2639
Hpy166II GTNNAC 8 cut(s) 65, 367, 511, 1058, 1253, 1732, 2124, 2490
Hpy8I GTNNAC 8 cut(s) 65, 367, 511, 1058, 1253, 1732, 2124, 2490
HpyCH4IV ACGT 2 cut(s) 1314, 2168
HpyF10VI GCNNNNNNNGC 6 cut(s) 316, 1077, 1484, 2020, 2305, 2487
HpySE526I ACGT 2 cut(s) 1314, 2168
KspAI GTTAAC 1 cut(s) 511
LguI GCTCTTC 4 cut(s) 255, 320, 1559, 2265
LmnI GCTCC 3 cut(s) 1483, 1804, 2797
Lsp1109I GCAGC 2 cut(s) 901, 1058
LweI GCATC 5 cut(s) 813, 2320, 2410, 2464, 2716
MaeI CTAG 7 cut(s) 527, 597, 1202, 1527, 1625, 2532, 2690
MaeII ACGT 2 cut(s) 1314, 2168
MaeIII GTNAC 5 cut(s) 698, 1110, 2147, 2384, 2521
MfeI CAATTG 1 cut(s) 2088
MflI RGATCY 2 cut(s) 776, 2662
MhlI GDGCHC 9 cut(s) 267, 369, 526, 1349, 1801, 2025, 2437, 2492, 2755
MlsI TGGCCA 1 cut(s) 548
MluNI TGGCCA 1 cut(s) 548
MlyI GAGTC 3 cut(s) 1916, 2460, 2640
MmeI TCCRAC 2 cut(s) 281, 2816
Mox20I TGGCCA 1 cut(s) 548
MroXI GAANNNNTTC 4 cut(s) 1444, 1765, 1984, 2209
MscI TGGCCA 1 cut(s) 548
MseI TTAA 7 cut(s) 35, 510, 740, 1491, 1650, 2081, 2649
Msp20I TGGCCA 1 cut(s) 548
MspA1I CMGCKG 3 cut(s) 200, 1931, 2831
MspCI CTTAAG 2 cut(s) 739, 1490
MspI CCGG 1 cut(s) 2639
MspR9I CCNGG 3 cut(s) 2318, 2771, 2796
MunI CAATTG 1 cut(s) 2088
MvaI CCWGG 3 cut(s) 2318, 2771, 2796
MwoI GCNNNNNNNGC 6 cut(s) 316, 1077, 1484, 2020, 2305, 2487
NdeI CATATG 1 cut(s) 1507
NlaIV GGNNCC 4 cut(s) 196, 892, 1310, 2315
NmeAIII GCCGAG 1 cut(s) 345
NmuCI GTSAC 2 cut(s) 698, 2384
NspI RCATGY 3 cut(s) 230, 490, 1132
PaeI GCATGC 1 cut(s) 1132
PaqCI CACCTGC 1 cut(s) 2290
PceI AGGCCT 1 cut(s) 460
PciSI GCTCTTC 4 cut(s) 255, 320, 1559, 2265
PdmI GAANNNNTTC 4 cut(s) 1444, 1765, 1984, 2209
PfeI GAWTC 8 cut(s) 421, 533, 643, 1043, 1586, 1964, 2075, 2291
PflFI GACNNNGTC 1 cut(s) 2630
PflMI CCANNNNNTGG 1 cut(s) 947
PkrI GCNGC 2 cut(s) 916, 1073
PleI GAGTC 3 cut(s) 1915, 2459, 2639
PpsI GAGTC 3 cut(s) 1915, 2459, 2639
Ppu21I YACGTR 1 cut(s) 1315
Psp124BI GAGCTC 1 cut(s) 2025
Psp6I CCWGG 3 cut(s) 2316, 2769, 2794
PspFI CCCAGC 2 cut(s) 2045, 2411
PspGI CCWGG 3 cut(s) 2316, 2769, 2794
PspN4I GGNNCC 4 cut(s) 196, 892, 1310, 2315
PspPI GGNCC 3 cut(s) 1309, 2695, 2744
PsrI GAACNNNNNNTAC 2 cut(s) 120, 152
PstNI CAGNNNCTG 2 cut(s) 914, 2611
PsuI RGATCY 2 cut(s) 776, 2662
PsyI GACNNNGTC 1 cut(s) 2630
PvuII CAGCTG 2 cut(s) 1931, 2831
RsaI GTAC 3 cut(s) 86, 1614, 2103
RsaNI GTAC 3 cut(s) 85, 1613, 2102
SacI GAGCTC 1 cut(s) 2025
SalI GTCGAC 1 cut(s) 2122
SapI GCTCTTC 4 cut(s) 255, 320, 1559, 2265
SaqAI TTAA 7 cut(s) 35, 510, 740, 1491, 1650, 2081, 2649
SatI GCNGC 2 cut(s) 915, 1072
Sau96I GGNCC 3 cut(s) 1309, 2695, 2744
SchI GAGTC 3 cut(s) 1916, 2460, 2640
ScrFI CCNGG 3 cut(s) 2318, 2771, 2796
SduI GDGCHC 9 cut(s) 267, 369, 526, 1349, 1801, 2025, 2437, 2492, 2755
SfaNI GCATC 5 cut(s) 813, 2320, 2410, 2464, 2716
SfcI CTRYAG 5 cut(s) 1482, 1728, 1969, 2028, 2819
SinI GGWCC 2 cut(s) 1309, 2744
SmlI CTYRAG 3 cut(s) 739, 1019, 1490
SmoI CTYRAG 3 cut(s) 739, 1019, 1490
SpeI ACTAGT 1 cut(s) 1624
SphI GCATGC 1 cut(s) 1132
SseBI AGGCCT 1 cut(s) 460
SsiI CCGC 4 cut(s) 198, 530, 767, 2693
SspI AATATT 2 cut(s) 981, 1444
SspMI CTAG 7 cut(s) 527, 597, 1202, 1527, 1625, 2532, 2690
SstI GAGCTC 1 cut(s) 2025
StuI AGGCCT 1 cut(s) 460
StyD4I CCNGG 3 cut(s) 2316, 2769, 2794
StyI CCWWGG 2 cut(s) 1449, 2531
TaiI ACGT 2 cut(s) 1317, 2171
TatI WGTACW 1 cut(s) 1612
TfiI GAWTC 8 cut(s) 421, 533, 643, 1043, 1586, 1964, 2075, 2291
Tru1I TTAA 7 cut(s) 35, 510, 740, 1491, 1650, 2081, 2649
Tru9I TTAA 7 cut(s) 35, 510, 740, 1491, 1650, 2081, 2649
TscAI CASTG 7 cut(s) 346, 374, 872, 2032, 2220, 2268, 2435
TseFI GTSAC 2 cut(s) 698, 2384
TseI GCWGC 2 cut(s) 914, 1071
Tsp45I GTSAC 2 cut(s) 698, 2384
TspDTI ATGAA 7 cut(s) 113, 123, 635, 740, 1052, 2150, 2238
TspGWI ACGGA 1 cut(s) 1431
TspRI CASTG 7 cut(s) 346, 374, 872, 2032, 2220, 2268, 2435
Tth111I GACNNNGTC 1 cut(s) 2630
Van91I CCANNNNNTGG 1 cut(s) 947
Vha464I CTTAAG 2 cut(s) 739, 1490
VneI GTGCAC 2 cut(s) 365, 2488
VpaK11BI GGWCC 2 cut(s) 1309, 2744
XagI CCTNNNNNAGG 1 cut(s) 287
XceI RCATGY 3 cut(s) 230, 490, 1132
XmaJI CCTAGG 1 cut(s) 2531
XmiI GTMKAC 3 cut(s) 1252, 1731, 2123
XmnI GAANNNNTTC 4 cut(s) 1444, 1765, 1984, 2209
XspI CTAG 7 cut(s) 527, 597, 1202, 1527, 1625, 2532, 2690
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.