MD03G1191000.v1.1

metal ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000343
Physical Location & Seq
Forward (+)
26261850 .. 26274142
12293 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1191000.v1.1.491

Sequence Viewer

Length: 3690 bp
ATGGAATCGTCAGACGATGAGGCGGAGGCGGTGCCGCAATCCGTTTCGAATTACCATTTTGTAGATGACAAGGACGAACCCATCTCATTTCATGTTTTGCCGATTCAGTGGAGTGAGGGTGAGACGCAAGATGGGAAGAAAGTGGACATATTTGTGCGTGGCACTGCCGATAATGGGCTTCAGAAACTGTATAAGCATGTTATCGCATGGCGGTTTGATCTTTCCAATGTCGATCCTGAGATATCGGTTCTCTCTAAGGAAAATAACTGGCTCAAGCTTCAGAAGCCGAGAAAGAGTTTCGAGGAAGTAATAAGTTCAATCTTGATAACAGTGCAGTGCCTTCACTATGTGAGGAGAAATCCTGAGACATCCGGCAAATCTCTGTGGGATCACTTATCTAAAGTTTTCAGCTCCTATGAGGTCAGGCCTTCTCAGAATGACCTGGTGAATTATATTCCTCTAATTAGTGAAGCTGTTAAACGGGATGATGCATTAGCAAAGTGCAAGTTTTTAGTCAACTTTCTTGAAGAGAAGCCCACCAAGAGGAAACTGCATGATGAGGACATTCAAGCTACAACAAAACCTGGATTTATAGTTGATGATACGGAAGAAGATATGATTGATGCTGAAGACGAGTCCAGTGACGATGATAACCTGTTTGATTCTGTTTGTGCATTTTGTGATAATGGTGGCGATCTTTTGTGTTGTGAAGGGAGATGCCTAAGGTCATTTCACGCAACTGTAAAGGATGGTGAGGATTCTATGTGTGAAACCCTTGGCTTTACCCAGGATGAAGTAGACGCGATTCAGAACTTCTTCTGTAAGAACTGTCAGTATAAACAACATCAGTGCTATGCTTGTGGGAAGCTAGGCTCCTCTGATAAATCTTCAGGGGCTGAGGTCTTCCCCTGTGTTTCTGCAACCTGTGGTCAATTTTACCATCCACGTTGCATTGCAAAACTCATTTATAAAGACAATGGAGTCTCTGCTGAAGAACTTGAGAAAAAAATTGCTATGGGTGAATCTTTTACATGTCCGATTCATAAATGCTGCATTTGTAAACAAGGAGAGAACAAGAAGGATACTCAGTTGCGGTTTGCTGTGTGTCGGCGTTGTCCCAAATCCTACCACAGGAAATGCTTGCCAGAGGATATTGTATTTGAGAAAACAGAGAAAACAGAGAAAACAGAGGAAGACGAGGAGGAGGAAGACGAAGAGGATGAAGGTACAACACCAAGGGCTTGGGAAGGACTATTACCAAACCGTGTACTAATATATTGCACAAAACATGAGATAATTAAAGACATTGGAACGCCAATAAGGGACCACGTAACATTCCCTGATGTTGGAGAAAAGAGGACCAGTTTTGTTCGAAAGAAGACTGATTTTGTAGAGAAAAAGAAAAAACGGACATTAGAATCCCTTCAAGATAGAGAAAAATCTGTGAAAAATAAGAGAAGCCTTTCCGCTGAAGAATTCTGTAGGGGTCAAACTGCTCCTACGATATCTAAAGAGAAGCTGAAGTCATCTTCTGCTGCAAAAGTGGGTGGTAACAGAATTAGTAAAAAACTACCTTCTGGGTTAGATACATCAAGAAAGGTGAAAGCGAATAGTGCTTTGAAAAAGGAAGCTAAGATTTCTGTGGCTGAGGAACAAATGACCTCATTGGGTGATCAGCTATATGCCTATATGAACAGGTCTAATCAAGTGAAGTTGGGTAAGCAGGGCAAACCTGATGGGGAGTGTGGTTTGGCAATAGGCAATCCTGCCTCAAAAACGTTGATCAGTGCACCACCATCTTTAGATGCAGCTACAGAGAGGAGATTACTGGCTTTAATGAAAGATGCCACATCTTCAATAACTCTGGAAGATGTTATAAGAAAGCGGCAGCGCACAGTTCCATCGACTCATCAATCCTCGTCCAAAAATGCTGTGGATAGGAACATTACACTGGGAAAGGTAGAAGGCTCAGTTGAGGCTGTTCGAACAGCGTTACGGAAACTAGAGGAAGGATGCAGTACTGAAGATTCTGAGGCTGTTTGTGCTCCTGAGGTGGTTCACCAGATTTACAAGTGGAAGAACAAGCTGAGAGTATATCTTGCACCTTTTTTGCACGGAATGCGCTACACGTCCTTTGGTCGTCATTTTACAAAGGTGGACAAATTAGAAGAGATTGCTGATAGGCTGCATTGGTACGTAAAAGATGGAGATACGATAGTGGACTTCTGTTGTGGTGCCAATGATTTCAGCATCGTAATGAAGAAAAAGCTTGAAGAGACGGGGAAGAACTGCTTTTATAAAAACTATGACTTAATTCAACCGAAGAACGATTTCTGTTTTGAGAAGAGAGATTGGATGAAAGTAAAACCAAAGGAGTTACCGAAAGGGTCACAATTGATCATGGGGTTGAACCCTCCCTTCGGAGTTAAAGCAGCTCTGGCAAACAGGTTTATCGACAAGGCTCTTGAGTTCGATCCAAAGCTCCTTATTCTTATTGTTCCACCAGAAACTCAAAGGTTAAATGAAAAGAAATCGCCATATGATCTAATCTGGGAGGATAACCAGTTTCTGTCAGGAAAGTCTTTTTATCTCCCGGGATCTGTTGATGTGAATGACAAACAAATGGATCAATGGAATGTAACGCCGCCACCACTCTATCTCTGGAGTCGTCCTGATTGGTCTGCTGACATCAAGGCTATAGCTCAAGAGCATGGTCACATGTCTGCATCACAAAGGTATATGAAAGATCATTCTGACTCTCTAAATCATGGTCGTTCAATAGGTAATGATGATCAATATGGTGAAGCACCTATGCTGATTGATTATGATGGCATAAAAACCGATAGTCCCAAAGATGTTGAAGGTGGGGCTGTTGTGGCTGAGGAACACAGAGAAAGCTCCTGTGAGAACAGTGGTGACATAGGTGGCAACGAGAGTCCTGGGGATGGTAACAACATTGATGAGACATGCAATGAAATTCTGCCACGCATTGAGCCTGCTGAAAAGGGAGATCAGCACTCTGAGCCTAGCAATTCTGGTTCAAGCGCGAAGTACGGAACTACTTATGGTGGGACAACGGTTAATGTAGCTGATGACAGAGGTAGAAGAAGCTTATCGAGGAGCAGTGATGAGCCTTACTCGAGTCTGACTCATAGATGGTCCGCTGGTCCCAGTTCTGGTTATAGGGCTACAAACTTGGAGGAACCGTTTGTGGGGCACATGAGAGACAGATTGGATACCCTTGGATATAGACCATGCTTGAATGAGGTAGAAGATCCATTTAGGAGGGAGTCTGACGTACGTTCTCAGATTCGGATTTATGGTCAGCAAGATTTTGGTCCTTTAAGAAGTAATTATCTAGTGGGTCAGGATTCTGTAAGTGGACAGATTGGATCGTATTCATCTCCTTACAGCCATAGTCATCTTGGTCTGACAGCTGAGTCATCTTACAGAATGAACACGTCCGCCATGCAGCGGTATGCACCTCGGTTGGATGAACTGAACCACACGAGGATGGGTGGCGTAGGATCTGAACCTGCTTTAGGATACGAACCGCACATGTTCAGCAGCAACGGCACTTTTGATCCCAGGGCACCCCGGCCCGGTCAACACGGTGGCTCCATGGGTTTTGCTCCCGGTCCTCACCAAAGCTATTCGAACCAAAATTCAGCAGGTTGGTTGAATGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006325 GO:0006355 GO:0006950 GO:0006952 GO:0006996 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0009605 GO:0009607 GO:0009620 GO:0009791 GO:0009889 GO:0009893 GO:0009909 GO:0009911 GO:0009987 GO:0010228 GO:0010468 GO:0010556 GO:0010604 GO:0010638 GO:0016043 GO:0019219 GO:0019222 GO:0022414 GO:0023052 GO:0030154 GO:0031056 GO:0031058 GO:0031060 GO:0031062 GO:0031323 GO:0031325 GO:0031326 GO:0031399 GO:0031401 GO:0031440 GO:0031935 GO:0031937 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033043 GO:0033044 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0043954 GO:0044030 GO:0044424 GO:0044464 GO:0048366 GO:0048367 GO:0048468 GO:0048518 GO:0048522 GO:0048580 GO:0048582 GO:0048608 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0048869 GO:0050684 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051052 GO:0051094 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051276 GO:0051570 GO:0051574 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060968 GO:0061085 GO:0061087 GO:0061458 GO:0065007 GO:0070827 GO:0070828 GO:0070829 GO:0071840 GO:0080090 GO:0090436 GO:0097159 GO:0098542 GO:0099402 GO:0140110 GO:1900109 GO:1900111 GO:1900363 GO:1901363 GO:1902275 GO:1903311 GO:1903506 GO:1905269 GO:2000024 GO:2000026 GO:2000112 GO:2000241 GO:2000243 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNMT1-RFD

Protein Analysis

1230

Amino Acids

137.98

Weight (kDa)

5.77

Isoelectric Point (pI)

44.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNMT1-RFD PF12047 14 - 137 4e-12 Cytosine specific DNA methyltransferase replication foci domain
PHD_NSD PF22908 280 - 348 1.7e-19 Histone-lysine N-methyltransferase NSD-like, PHD zinc finger
Mtase_EDM2 PF26055 732 - 902 1.8e-93 EDM2 methyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000343)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48090 AT5G48090 AT5G48090 AT5G55390 AT5G55390 AT5G55390
fragaria_vesca FvH4_3g23690 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160
malus_domestica MD03G1191000.v1.1 MD11G1207800.v1.1 MD11G1208000.v1.1
prunus_persica Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227600_v2.0.a1 Prupe.4G227700_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1
pyrus_communis pycom01g03400 pycom03g14420 pycom11g18100 pycom11g18140 pycom11g18150 pycom11g18160
rosa_chinensis RchiOBHm_Chr2g0145981 RchiOBHm_Chr4g0405241 RchiOBHm_Chr4g0405271 RchiOBHm_Chr5g0016201 RchiOBHm_Chr5g0041831 RchiOBHm_Chr5g0041871 RchiOBHm_Chr5g0041881 RchiOBHm_Chr5g0059241
rosa_laevigata RLG00000002117 RLG00000008861 RLG00000008862 RLG00000011000 RLG00000033216 RLG00000034097 RLG00000034101
rosa_multiflora Rmu_sc0000161.1_g000001 Rmu_sc0000861.1_g000040 Rmu_sc0007522.1_g000003 Rmu_sc0009443.1_g000015 Rmu_sc0022129.1_g000001 Rmu_sc0042315.1_g000001
rosa_roxburghii Rroxscaffold_1G00038660 Rroxscaffold_1G00038690 Rroxscaffold_5G00349560 Rroxscaffold_5G00349610 Rroxscaffold_6G00388370 Rroxscaffold_7G00188740
rosa_rugosa Rorug03G0199700 Rorug04G0055400 Rorug04G0055500 Rorug04G0055900 Rorug04G0056000 Rorug04G0056100 Rorug04G0056100 Rorug04G0056200 Rorug05G0195600 Rorug05G0195700 Rorug05G0195800 Rorug05G0195900 Rorug05G0196800 Rorug05G0385000
rosa_samantha Rh4AG129900 Rh4AG130100 Rh4BG124200 Rh4BG124400 Rh4BG124500 Rh4CG137200 Rh4CG137300 Rh4DG123500 Rh4DG123700 Rh5AG280800 Rh5AG281400 Rh5BG286700 Rh5BG287000 Rh5BG397800 Rh5CG318100 Rh5CG318600 Rh5CG421600 Rh5DG132300 Rh5DG132400 Rh5DG295400 Rh5DG295700 Rh7BG475300
rosa_wichuraiana Rw0G001330 Rw0G005730 Rw4G008670 Rw4G010510 Rw4G010540 Rw5G026450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 969, 1877, 2298
AasI GACNNNNNNGTC 2 cut(s) 980, 3439
Acc36I ACCTGC 2 cut(s) 3544, 3662
AccB1I GGYRCC 3 cut(s) 31, 2234, 3592
AccI GTMKAC 1 cut(s) 798
AccII CGCG 2 cut(s) 803, 3047
AclI AACGTT 1 cut(s) 1778
AclWI GGATC 9 cut(s) 227, 396, 2468, 2605, 2634, 3269, 3400, 3535, 3578
AcsI RAATTY 3 cut(s) 1475, 2976, 3664
AcuI CTGAAG 8 cut(s) 164, 263, 648, 873, 1011, 1491, 1541, 2043
AdeI CACNNNGTG 1 cut(s) 349
AfaI GTAC 6 cut(s) 1228, 1269, 2020, 2195, 3053, 3300
AflIII ACRYGT 5 cut(s) 1031, 2127, 2718, 3459, 3558
AjiI CACGTC 2 cut(s) 2130, 3462
AjnI CCWGG 5 cut(s) 441, 583, 786, 2938, 3587
Alw21I GWGCWC 2 cut(s) 1792, 2047
Alw26I GTCTC 6 cut(s) 116, 359, 988, 2270, 2957, 3219
Alw44I GTGCAC 1 cut(s) 1788
AlwI GGATC 9 cut(s) 227, 396, 2468, 2605, 2634, 3269, 3400, 3535, 3578
AlwNI CAGNNNCTG 3 cut(s) 187, 896, 2569
Ama87I CYCGRG 2 cut(s) 2593, 3139
AoxI GGCC 2 cut(s) 425, 3599
ApaLI GTGCAC 1 cut(s) 1788
ApeKI GCWGC 8 cut(s) 1050, 1535, 1808, 1888, 2185, 2432, 3472, 3567
ApoI RAATTY 3 cut(s) 1475, 2976, 3664
Asp700I GAANNNNTTC 4 cut(s) 815, 1422, 1462, 2330
AspLEI GCGC 3 cut(s) 1893, 2124, 3047
AspS9I GGNCC 7 cut(s) 1324, 1359, 3159, 3167, 3338, 3600, 3638
AsuC2I CCSGG 5 cut(s) 2594, 2595, 3598, 3603, 3636
AsuII TTCGAA 4 cut(s) 47, 1372, 1984, 3656
AvaI CYCGRG 2 cut(s) 2593, 3139
AvaII GGWCC 6 cut(s) 1324, 1359, 3159, 3167, 3338, 3638
AxyI CCTNAGG 2 cut(s) 722, 2049
BaeGI GKGCMC 3 cut(s) 1792, 3219, 3595
BaeI ACNNNNGTAYC 2 cut(s) 2202, 2235
BanI GGYRCC 3 cut(s) 31, 2234, 3592
BarI GAAGNNNNNNTAC 2 cut(s) 1239, 1271
BauI CACGAG 1 cut(s) 3508
BbsI GAAGAC 5 cut(s) 636, 895, 1200, 1215, 1385
Bbv12I GWGCWC 2 cut(s) 1792, 2047
BbvCI CCTCAGC 3 cut(s) 897, 1647, 2880
BbvI GCAGC 8 cut(s) 1037, 1522, 1820, 1900, 2172, 2444, 3484, 3579
BceAI ACGGC 1 cut(s) 3589
BciT130I CCWGG 5 cut(s) 443, 585, 788, 2940, 3589
BciVI GTATCC 3 cut(s) 1075, 3229, 3539
BclI TGATCA 4 cut(s) 1672, 1782, 2397, 2791
BcnI CCSGG 5 cut(s) 2594, 2595, 3598, 3603, 3636
BcoDI GTCTC 6 cut(s) 116, 359, 988, 2270, 2957, 3219
BfaI CTAG 4 cut(s) 869, 2003, 3027, 3359
BfmI CTRYAG 3 cut(s) 1480, 1812, 2697
BfuAI ACCTGC 2 cut(s) 3544, 3662
BfuI GTATCC 3 cut(s) 1075, 3229, 3539
BmcAI AGTACT 1 cut(s) 2020
Bme18I GGWCC 6 cut(s) 1324, 1359, 3159, 3167, 3338, 3638
BmeT110I CYCGRG 2 cut(s) 2593, 3139
BmgBI CACGTC 2 cut(s) 2130, 3462
BmgT120I GGNCC 7 cut(s) 1324, 1359, 3159, 3167, 3338, 3600, 3638
BmiI GGNNCC 8 cut(s) 33, 874, 1325, 2236, 3169, 3204, 3594, 3619
BmrI ACTGGG 2 cut(s) 1961, 3165
BmsI GCATC 8 cut(s) 478, 613, 707, 1795, 1834, 2003, 2259, 2735
BmuI ACTGGG 2 cut(s) 1961, 3165
BpiI GAAGAC 5 cut(s) 636, 895, 1200, 1215, 1385
BplI GAGNNNNNCTC 4 cut(s) 3122, 3154, 3133, 3165
BpmI CTGGAG 1 cut(s) 2683
Bpu10I CCTNAGC 3 cut(s) 897, 1647, 2880
Bpu14I TTCGAA 4 cut(s) 47, 1372, 1984, 3656
BpuEI CTTGAG 4 cut(s) 257, 1019, 2486, 2688
BpuMI CCSGG 5 cut(s) 2594, 2595, 3598, 3603, 3636
BsaAI YACGTR 2 cut(s) 1330, 2197
BsaXI ACNNNNNCTCC 6 cut(s) 103, 133, 3191, 3221, 3602, 3632
Bse1I ACTGG 7 cut(s) 272, 639, 1362, 1833, 1956, 2563, 3171
Bse21I CCTNAGG 2 cut(s) 722, 2049
Bse3DI GCAATG 2 cut(s) 951, 2977
BseBI CCWGG 5 cut(s) 443, 585, 788, 2940, 3589
BseMI GCAATG 2 cut(s) 951, 2977
BseNI ACTGG 7 cut(s) 272, 639, 1362, 1833, 1956, 2563, 3171
BseRI GAGGAG 6 cut(s) 367, 865, 1214, 1217, 1834, 3133
BseSI GKGCMC 3 cut(s) 1792, 3219, 3595
BseXI GCAGC 8 cut(s) 1037, 1522, 1820, 1900, 2172, 2444, 3484, 3579
BsgI GTGCAG 1 cut(s) 353
Bsh1236I CGCG 2 cut(s) 803, 3047
BshFI GGCC 2 cut(s) 427, 3601
BshNI GGYRCC 3 cut(s) 31, 2234, 3592
BsiHKAI GWGCWC 2 cut(s) 1792, 2047
BsiHKCI CYCGRG 2 cut(s) 2593, 3139
BsiSI CCGG 5 cut(s) 372, 2594, 3598, 3603, 3636
BsiWI CGTACG 1 cut(s) 3298
BslFI GGGAC 5 cut(s) 1101, 1337, 2832, 3085, 3153
BsmAI GTCTC 6 cut(s) 116, 359, 988, 2270, 2957, 3219
BsmBI CGTCTC 2 cut(s) 116, 2270
BsmFI GGGAC 5 cut(s) 1101, 1337, 2832, 3085, 3153
BsmI GAATGC 1 cut(s) 2124
BsnI GGCC 2 cut(s) 427, 3601
BsoBI CYCGRG 2 cut(s) 2593, 3139
Bsp119I TTCGAA 4 cut(s) 47, 1372, 1984, 3656
Bsp1286I GDGCHC 4 cut(s) 1792, 2047, 3219, 3595
Bsp19I CCATGG 1 cut(s) 3621
BspANI GGCC 2 cut(s) 427, 3601
BspFNI CGCG 2 cut(s) 803, 3047
BspLI GGNNCC 8 cut(s) 33, 874, 1325, 2236, 3169, 3204, 3594, 3619
BspMI ACCTGC 2 cut(s) 3544, 3662
BspPI GGATC 9 cut(s) 227, 396, 2468, 2605, 2634, 3269, 3400, 3535, 3578
BspT104I TTCGAA 4 cut(s) 47, 1372, 1984, 3656
BspT107I GGYRCC 3 cut(s) 31, 2234, 3592
BsrDI GCAATG 2 cut(s) 951, 2977
BsrI ACTGG 7 cut(s) 272, 639, 1362, 1833, 1956, 2563, 3171
BssSI CACGAG 1 cut(s) 3508
BssT1I CCWWGG 4 cut(s) 775, 1235, 3241, 3621
Bst2BI CACGAG 1 cut(s) 3508
Bst2UI CCWGG 5 cut(s) 443, 585, 788, 2940, 3589
Bst6I CTCTTC 5 cut(s) 522, 1209, 2163, 2268, 2339
BstAPI GCANNNNNTGC 1 cut(s) 2119
BstBAI YACGTR 2 cut(s) 1330, 2197
BstBI TTCGAA 4 cut(s) 47, 1372, 1984, 3656
BstC8I GCNNGC 2 cut(s) 1142, 2997
BstDSI CCRYGG 1 cut(s) 3621
BstENI CCTNNNNNAGG 2 cut(s) 1129, 3540
BstFNI CGCG 2 cut(s) 803, 3047
BstHHI GCGC 3 cut(s) 1893, 2124, 3047
BstMAI GTCTC 6 cut(s) 116, 359, 988, 2270, 2957, 3219
BstMWI GCNNNNNNNGC 8 cut(s) 283, 1613, 2042, 2119, 2438, 2876, 3022, 3573
BstNI CCWGG 5 cut(s) 443, 585, 788, 2940, 3589
BstNSI RCATGY 5 cut(s) 200, 1035, 2722, 2970, 3562
BstSFI CTRYAG 3 cut(s) 1480, 1812, 2697
BstSLI GKGCMC 3 cut(s) 1792, 3219, 3595
BstSNI TACGTA 1 cut(s) 2197
BstUI CGCG 2 cut(s) 803, 3047
BstV1I GCAGC 8 cut(s) 1037, 1522, 1820, 1900, 2172, 2444, 3484, 3579
BstV2I GAAGAC 5 cut(s) 636, 895, 1200, 1215, 1385
BstX2I RGATCY 3 cut(s) 2597, 3274, 3527
BstXI CCANNNNNNTGG 1 cut(s) 1242
BstYI RGATCY 3 cut(s) 2597, 3274, 3527
Bsu36I CCTNAGG 2 cut(s) 722, 2049
BsuI GTATCC 3 cut(s) 1075, 3229, 3539
BsuRI GGCC 2 cut(s) 427, 3601
BtgI CCRYGG 1 cut(s) 3621
BtrI CACGTC 2 cut(s) 2130, 3462
BtsI GCAGTG 3 cut(s) 162, 341, 3130
BveI ACCTGC 2 cut(s) 3544, 3662
Cac8I GCNNGC 2 cut(s) 1142, 2997
CaiI CAGNNNCTG 3 cut(s) 187, 896, 2569
CfoI GCGC 3 cut(s) 1893, 2124, 3047
Cfr13I GGNCC 7 cut(s) 1324, 1359, 3159, 3167, 3338, 3600, 3638
Cfr9I CCCGGG 1 cut(s) 2593
CseI GACGC 2 cut(s) 133, 809
CsiI ACCWGGT 1 cut(s) 441
Csp6I GTAC 6 cut(s) 1227, 1268, 2019, 2194, 3052, 3299
CviQI GTAC 6 cut(s) 1227, 1268, 2019, 2194, 3052, 3299
DraIII CACNNNGTG 1 cut(s) 349
DrdI GACNNNNNNGTC 2 cut(s) 980, 3439
DseDI GACNNNNNNGTC 2 cut(s) 980, 3439
Eam1104I CTCTTC 5 cut(s) 522, 1209, 2163, 2268, 2339
EarI CTCTTC 5 cut(s) 522, 1209, 2163, 2268, 2339
EciI GGCGGA 2 cut(s) 38, 3454
Eco105I TACGTA 1 cut(s) 2197
Eco130I CCWWGG 4 cut(s) 775, 1235, 3241, 3621
Eco147I AGGCCT 1 cut(s) 427
Eco32I GATATC 2 cut(s) 243, 1506
Eco47I GGWCC 6 cut(s) 1324, 1359, 3159, 3167, 3338, 3638
Eco57I CTGAAG 8 cut(s) 164, 263, 648, 873, 1011, 1491, 1541, 2043
Eco81I CCTNAGG 2 cut(s) 722, 2049
Eco88I CYCGRG 2 cut(s) 2593, 3139
EcoNI CCTNNNNNAGG 2 cut(s) 1129, 3540
EcoRI GAATTC 1 cut(s) 1475
EcoRII CCWGG 5 cut(s) 441, 583, 786, 2938, 3587
EcoRV GATATC 2 cut(s) 243, 1506
EcoT14I CCWWGG 4 cut(s) 775, 1235, 3241, 3621
EcoT22I ATGCAT 1 cut(s) 493
ErhI CCWWGG 4 cut(s) 775, 1235, 3241, 3621
Esp3I CGTCTC 2 cut(s) 116, 2270
FalI AAGNNNNNCTT 2 cut(s) 2276, 2308
FaqI GGGAC 5 cut(s) 1101, 1337, 2832, 3085, 3153
FauNDI CATATG 1 cut(s) 2539
FbaI TGATCA 4 cut(s) 1672, 1782, 2397, 2791
FblI GTMKAC 1 cut(s) 798
FspBI CTAG 4 cut(s) 869, 2003, 3027, 3359
GlaI GCGC 3 cut(s) 1892, 2123, 3046
GsuI CTGGAG 1 cut(s) 2683
HaeIII GGCC 2 cut(s) 427, 3601
HapII CCGG 5 cut(s) 372, 2594, 3598, 3603, 3636
HgaI GACGC 2 cut(s) 133, 809
HhaI GCGC 3 cut(s) 1893, 2124, 3047
Hin6I GCGC 3 cut(s) 1891, 2122, 3045
HinP1I GCGC 3 cut(s) 1891, 2122, 3045
HincII GTYRAC 2 cut(s) 517, 3608
HindII GTYRAC 2 cut(s) 517, 3608
HindIII AAGCTT 3 cut(s) 275, 2267, 3109
HpaII CCGG 5 cut(s) 372, 2594, 3598, 3603, 3636
HpyCH4IV ACGT 8 cut(s) 946, 1329, 1778, 2129, 2196, 3297, 3301, 3461
HpyF10VI GCNNNNNNNGC 8 cut(s) 283, 1613, 2042, 2119, 2438, 2876, 3022, 3573
HpySE526I ACGT 8 cut(s) 946, 1329, 1778, 2129, 2196, 3297, 3301, 3461
HspAI GCGC 3 cut(s) 1891, 2122, 3045
Ksp22I TGATCA 4 cut(s) 1672, 1782, 2397, 2791
LmnI GCTCC 9 cut(s) 416, 878, 1501, 2050, 2487, 2903, 3120, 3623, 3637
Lsp1109I GCAGC 8 cut(s) 1037, 1522, 1820, 1900, 2172, 2444, 3484, 3579
LweI GCATC 8 cut(s) 478, 613, 707, 1795, 1834, 2003, 2259, 2735
MabI ACCWGGT 1 cut(s) 441
MaeI CTAG 4 cut(s) 869, 2003, 3027, 3359
MaeII ACGT 8 cut(s) 946, 1329, 1778, 2129, 2196, 3297, 3301, 3461
MfeI CAATTG 1 cut(s) 2393
MflI RGATCY 3 cut(s) 2597, 3274, 3527
MhlI GDGCHC 4 cut(s) 1792, 2047, 3219, 3595
MmeI TCCRAC 2 cut(s) 1327, 3471
Mph1103I ATGCAT 1 cut(s) 493
MroXI GAANNNNTTC 4 cut(s) 815, 1422, 1462, 2330
MseI TTAA 8 cut(s) 477, 1299, 1835, 2312, 2427, 2519, 3081, 3344
MslI CAYNNNNRTG 3 cut(s) 152, 2255, 3512
MspA1I CMGCKG 4 cut(s) 1469, 3164, 3437, 3475
MspI CCGG 5 cut(s) 372, 2594, 3598, 3603, 3636
MunI CAATTG 1 cut(s) 2393
Mva1269I GAATGC 1 cut(s) 2124
MvaI CCWGG 5 cut(s) 443, 585, 788, 2940, 3589
MvnI CGCG 2 cut(s) 803, 3047
MwoI GCNNNNNNNGC 8 cut(s) 283, 1613, 2042, 2119, 2438, 2876, 3022, 3573
NciI CCSGG 5 cut(s) 2594, 2595, 3598, 3603, 3636
NcoI CCATGG 1 cut(s) 3621
NdeI CATATG 1 cut(s) 2539
NlaIV GGNNCC 8 cut(s) 33, 874, 1325, 2236, 3169, 3204, 3594, 3619
NmeAIII GCCGAG 1 cut(s) 312
NmuCI GTSAC 4 cut(s) 641, 2388, 2714, 2915
NsiI ATGCAT 1 cut(s) 493
NspI RCATGY 5 cut(s) 200, 1035, 2722, 2970, 3562
NspV TTCGAA 4 cut(s) 47, 1372, 1984, 3656
PaeR7I CTCGAG 1 cut(s) 3139
PasI CCCWGGG 1 cut(s) 3588
PceI AGGCCT 1 cut(s) 427
PciI ACATGT 3 cut(s) 1031, 2718, 3558
PctI GAATGC 1 cut(s) 2124
PdmI GAANNNNTTC 4 cut(s) 815, 1422, 1462, 2330
Pfl23II CGTACG 1 cut(s) 3298
Ppu21I YACGTR 2 cut(s) 1330, 2197
PscI ACATGT 3 cut(s) 1031, 2718, 3558
PsiI TTATAA 3 cut(s) 969, 1877, 2298
Psp1406I AACGTT 1 cut(s) 1778
Psp6I CCWGG 5 cut(s) 441, 583, 786, 2938, 3587
PspGI CCWGG 5 cut(s) 441, 583, 786, 2938, 3587
PspLI CGTACG 1 cut(s) 3298
PspN4I GGNNCC 8 cut(s) 33, 874, 1325, 2236, 3169, 3204, 3594, 3619
PspPI GGNCC 7 cut(s) 1324, 1359, 3159, 3167, 3338, 3600, 3638
PspXI VCTCGAGB 1 cut(s) 3139
PstNI CAGNNNCTG 3 cut(s) 187, 896, 2569
PsuI RGATCY 3 cut(s) 2597, 3274, 3527
PvuII CAGCTG 1 cut(s) 3437
RsaI GTAC 6 cut(s) 1228, 1269, 2020, 2195, 3053, 3300
RsaNI GTAC 6 cut(s) 1227, 1268, 2019, 2194, 3052, 3299
RseI CAYNNNNRTG 3 cut(s) 152, 2255, 3512
SaqAI TTAA 8 cut(s) 477, 1299, 1835, 2312, 2427, 2519, 3081, 3344
Sau96I GGNCC 7 cut(s) 1324, 1359, 3159, 3167, 3338, 3600, 3638
ScaI AGTACT 1 cut(s) 2020
SduI GDGCHC 4 cut(s) 1792, 2047, 3219, 3595
SexAI ACCWGGT 1 cut(s) 441
SfaNI GCATC 8 cut(s) 478, 613, 707, 1795, 1834, 2003, 2259, 2735
SfcI CTRYAG 3 cut(s) 1480, 1812, 2697
Sfr274I CTCGAG 1 cut(s) 3139
SfuI TTCGAA 4 cut(s) 47, 1372, 1984, 3656
SinI GGWCC 6 cut(s) 1324, 1359, 3159, 3167, 3338, 3638
SlaI CTCGAG 1 cut(s) 3139
SmaI CCCGGG 1 cut(s) 2595
SmiMI CAYNNNNRTG 3 cut(s) 152, 2255, 3512
SmlI CTYRAG 5 cut(s) 272, 998, 2465, 2703, 3139
SmoI CTYRAG 5 cut(s) 272, 998, 2465, 2703, 3139
SnaBI TACGTA 1 cut(s) 2197
SseBI AGGCCT 1 cut(s) 427
SspMI CTAG 4 cut(s) 869, 2003, 3027, 3359
StuI AGGCCT 1 cut(s) 427
StyI CCWWGG 4 cut(s) 775, 1235, 3241, 3621
TaiI ACGT 8 cut(s) 949, 1332, 1781, 2132, 2199, 3300, 3304, 3464
TatI WGTACW 2 cut(s) 1267, 2018
TauI GCSGC 3 cut(s) 37, 1888, 2647
Tru1I TTAA 8 cut(s) 477, 1299, 1835, 2312, 2427, 2519, 3081, 3344
Tru9I TTAA 8 cut(s) 477, 1299, 1835, 2312, 2427, 2519, 3081, 3344
TseFI GTSAC 4 cut(s) 641, 2388, 2714, 2915
TseI GCWGC 8 cut(s) 1050, 1535, 1808, 1888, 2185, 2432, 3472, 3567
Tsp45I GTSAC 4 cut(s) 641, 2388, 2714, 2915
TspGWI ACGGA 6 cut(s) 31, 620, 1423, 2011, 2130, 3069
TspMI CCCGGG 1 cut(s) 2593
VneI GTGCAC 1 cut(s) 1788
VpaK11BI GGWCC 6 cut(s) 1324, 1359, 3159, 3167, 3338, 3638
XagI CCTNNNNNAGG 2 cut(s) 1129, 3540
XapI RAATTY 3 cut(s) 1475, 2976, 3664
XceI RCATGY 5 cut(s) 200, 1035, 2722, 2970, 3562
XcmI CCANNNNNNNNNTGG 3 cut(s) 1930, 2658, 3422
XhoI CTCGAG 1 cut(s) 3139
XmaI CCCGGG 1 cut(s) 2593
XmiI GTMKAC 1 cut(s) 798
XmnI GAANNNNTTC 4 cut(s) 815, 1422, 1462, 2330
XspI CTAG 4 cut(s) 869, 2003, 3027, 3359
ZrmI AGTACT 1 cut(s) 2020
Zsp2I ATGCAT 1 cut(s) 493
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.