Rh5AG280800

metal ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000343
Physical Location & Seq
Reverse (-)
38009128 .. 38021329
12202 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG280800.1

Sequence Viewer

Length: 3429 bp
ATGGCTGCTTCACTCCTTTTTCTAAGTTTAATTACCGGCTCCCCTTCGCCGTTCATCGATCAACAATATACCAAGCTTTCAGGGGTGAAAGAAGTGTTTGAATTCAAGGAGGAGATGGAATCGTCAGATGATGAGGCTGAGGCAATGCAGCTTTCGGTTTCGAATTACTACTTTGTAGATGGCAAGGAAGATCCTATCTCCTTTCATGTACTGCCAATTCAATGGAGTGAGGGTGATAGGCAGGATGGGAAGGAAGCAGTGTTGTTTCTGCATGGCAGTGCCGACAATGGGCTCGAGAAGATTTACAAACCTGTTGTTGCTTGGAAGTTTGATCTATCGAATGTCAAGCCCGAGATTGCAGTGCTTTCCAAGGAGAATGATTGGATTCTGCTCCAGAAGCCGAGGAGGAGCTATGAGGGGATCGTAAGGACTGTCTTGATAACTGTGCAGTGCCTAAGTTATGCAATGTGGAATGCTGAGGCGTCCGGAAAAGCTGTGTGGGATCACTTGTCTAAAGCGTTCAGTTGTGAAGGGAGATGCCTAAGGTCCTTCCATCCCACGGTAGAGGACGGCGAGGACTCAATGTGTGAATCTCTTGGATTCACTCGGGAGGAAGTAGATGCAATGGCAAGTTTCTTCTGCAAGAACTGTCAATATAAGCAGCATCAGTGCTTTGCTTGTGGGAAGTTAGGCTCCTCAGACAAATCTTTGGGTGCAGAGGTCTTCCCCTGTGTTTCTGCAACCTGTGGTCAATTTTACCATCCTCATTGTGTTGCACAACTAATTTATCAAGACAATGGACTTTCTGCTGAAGAACTGGAGAAAAATATCTCTCTGGGGGAGTCTTTTACGTGTCCGATTCATAAGTGCTGTGTTTGTAAGCAAGGAGAGAATAAGAAGGATCCCGAGTTACGGTTTGCTGTGTGCAGGCGTTGTCCTAAATCCTATCACAGGAAATGCTTGCCCAGAGAACACGAAATAGATGAAGAAATTGGAACTCCAATAAGGGACCATGTTAAATTTCCCGATGTTGACGGAAAGAAGACTAGTATTGTGAAAAGGAAGACTGCTTTTGAACAGAAAAAGAGAAGACTGACATCAGAGTCCCTTGTGGTCAGAGATAATTCTCTGTCGAAGAAAAGAAAGCTTTCCTCAGAGGGATTTTATAGTGGAAGAACTGCACCTCCAATATCTAAGCAGAAATTGAATTCGGGTGAAAAAGTTGGCAGTAGCAGAAATACTGAAAAAGTACCTTCTCGGTTAGACGTATCAAGAAAGGTGATGGTTAATAAGACATTGAAAAAGGAAGTTCAGACTAGCGTGGAAAAGAAGACCTCGTTGGGTAATAGACTGTTTACCTACATAAAGGGATCTGGGTCAGTAAAGTTTGGGAAGCAGGATACACCTGATGATGCTGAACTAAATTCTAAAACGAGTGCAAAAATTGATCCTACATCAAGAACATTGAGTGCGGCAGCTTCCTTAGATCCTGCTAGAGAAAGGAGATTACATGCTTTAATGAAAGACGCTGCTTCATCAATAAGATTGGAAGATGTTATAGAAAAGCACAAAGTTCCATCCACTCATGCATCCTCAAATAAATATGCTGTAGAAAGGAACATAACACGAGGAAAGGTGGAAGGCTCAGTTGAGGCTGTTCGAACCGCATTAAAGAAACTACAGGAAGGCTGCAGTACTGAAGATGCAGAGGCTGTCTGTGCTCCTGAGATTCTTTCCCAGATTTATAAGTGGAAGAGCAAGCTTAGAGTATATCTTGCACCTTTCTTGCATGGAAAGCGGTACACGTCCTTCGGTCGCCATTTCACAAAGGTGGAAAAACTCGAACAGATTGCTGATAGGCTCCATTCGTACGTACAGAATGGAGACACGATAGTGGACTTCTGTTGTGGCTCTAATGATTTCAGCATCGTAATGAAGAAAAAGCTTGAGGAGACAGGGAAGAAGTGCTATTTTAGAAATTATGACATTATTCAACCAAAGAATGATTTCTGTTTTGAGAAGAGAGATTGGATGACTGTTCAAAAAGGTGAATTACCGGATAGTAGTAAATTGATCATGGGGTTGAACCCTCCTTTTGGGGTTAAAGCATCTTTGGCAAACCAGTTTATTGATAAGGCTCTGCAGTTCCAGCCGAAGCTCCTTATTCTTATTGTTCCACCAGAAACGAAAGGGTTACAATGTTATGATCTGATATGGAAGGATGAACGGTCTCTGTCGGGAAAGTCCTTTTATCTTCCTGGATCTGTTGATGAGAATGACAAGCAAATGGATCAATGGAATGTGACGACACCTCCACTCTATCTTTGGAGTCGTCCTGATTGGTCTGAGAGACACAGAGCTATTGCTCAAAAGGATAGTGACGGTTCCATGTCAGGGTCAGTGGAAGATGGTCATTCAGTTGAGAATAAGGATGAAAATGTTGAAAATCTCATGTTGAAAAATGCTTATCTGACACCGACCGGTAATTCCAGAGATTTTGTTGGTGGGGCAGTTGCAGGCGAGGGACATGAAGATAGATCCTCCAAGGGCAATGATGACAGAGGTTACCATGCGAGTTCTGGTCACCAGAAGAACCAGGTTAGTAAGATTTCTGAGAGGCCGCGGTGTGGTGGCAGAAAAAAGAATGGGAGTGGAGTGGTTGAGTTGTCGCCAGACAACAAATGTGATGGTGGCAATGTTTCTAGTGAGATTCAGATGGAAAGGTCTCCATCTACTGAGCGACAGCGGAAAGCAAATCAGCAGTCTGAGCCCAGCGATTCTGGTTCAAGTATGCATTTCAGAACTGCTTGTGGGAGGACCACAGCCAGCATCCCTGATGACACTGGCAGCAATGTCACAAGCAGTGCTGACCCTTATTCAAGTGTGACTCATAGATGGTCCAGTGGTGCCAGTCCTAGCTCTATATATAGGGATACAAAATTGGAGGAACCTGTTTTGGGTTACATGAGAGAGACAGATAACTTCGGTTATAGACCGTATTCCATGGGGATGGAAGACCCATATAGGAGAGACTCCGATATACGCGCACAAGTTCGCCATTACGGGCAGCAAGATCCTGATATGTTCAGAAGTAATTATCTAGCAGGTCAGGATTTGTTACGTGGTCAGGTTGGATCATTTTCGTCCACGTATGGCCATGCTCATCTAGGTTCAGCTGTTGACTCATCTTACAGAATGAACATGTCAGCCATGCAGCGATATGAACCTCGGCTGGATGAATTGAACCACACAAGAACGAGTGCCTTAGGAACCCAACCAGCATTTGGGTATGATCCACGCACATACAGTAGCAACATGTATGATCCCAGGGCACCCCGACCTGGTGACCAAGGGGGCCCCTTTGGTTTTGCTCCCGGTTCTCACCAAAGCTTCTCAAACCGGAATTCAGCAGGTTGGCTGAACGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006325 GO:0006355 GO:0006950 GO:0006952 GO:0006996 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0009605 GO:0009607 GO:0009620 GO:0009791 GO:0009889 GO:0009893 GO:0009909 GO:0009911 GO:0009987 GO:0010228 GO:0010468 GO:0010556 GO:0010604 GO:0010638 GO:0016043 GO:0019219 GO:0019222 GO:0022414 GO:0023052 GO:0030154 GO:0031056 GO:0031058 GO:0031060 GO:0031062 GO:0031323 GO:0031325 GO:0031326 GO:0031399 GO:0031401 GO:0031440 GO:0031935 GO:0031937 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033043 GO:0033044 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0043954 GO:0044030 GO:0044424 GO:0044464 GO:0048366 GO:0048367 GO:0048468 GO:0048518 GO:0048522 GO:0048580 GO:0048582 GO:0048608 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0048869 GO:0050684 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051052 GO:0051094 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051276 GO:0051570 GO:0051574 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060968 GO:0061085 GO:0061087 GO:0061458 GO:0065007 GO:0070827 GO:0070828 GO:0070829 GO:0071840 GO:0080090 GO:0090436 GO:0097159 GO:0098542 GO:0099402 GO:0140110 GO:1900109 GO:1900111 GO:1900363 GO:1901363 GO:1902275 GO:1903311 GO:1903506 GO:1905269 GO:2000024 GO:2000026 GO:2000112 GO:2000241 GO:2000243 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNMT1-RFD

Protein Analysis

1142

Amino Acids

127.9

Weight (kDa)

8.23

Isoelectric Point (pI)

42.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNMT1-RFD PF12047 51 - 149 1.6e-06 Cytosine specific DNA methyltransferase replication foci domain
PHD_NSD PF22908 220 - 288 1.6e-19 Histone-lysine N-methyltransferase NSD-like, PHD zinc finger
Mtase_EDM2 PF26055 624 - 790 3.7e-88 EDM2 methyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000343)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48090 AT5G48090 AT5G48090 AT5G55390 AT5G55390 AT5G55390
fragaria_vesca FvH4_3g23690 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160
malus_domestica MD03G1191000.v1.1 MD11G1207800.v1.1 MD11G1208000.v1.1
prunus_persica Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227600_v2.0.a1 Prupe.4G227700_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1
pyrus_communis pycom01g03400 pycom03g14420 pycom11g18100 pycom11g18140 pycom11g18150 pycom11g18160
rosa_chinensis RchiOBHm_Chr2g0145981 RchiOBHm_Chr4g0405241 RchiOBHm_Chr4g0405271 RchiOBHm_Chr5g0016201 RchiOBHm_Chr5g0041831 RchiOBHm_Chr5g0041871 RchiOBHm_Chr5g0041881 RchiOBHm_Chr5g0059241
rosa_laevigata RLG00000002117 RLG00000008861 RLG00000008862 RLG00000011000 RLG00000033216 RLG00000034097 RLG00000034101
rosa_multiflora Rmu_sc0000161.1_g000001 Rmu_sc0000861.1_g000040 Rmu_sc0007522.1_g000003 Rmu_sc0009443.1_g000015 Rmu_sc0022129.1_g000001 Rmu_sc0042315.1_g000001
rosa_roxburghii Rroxscaffold_1G00038660 Rroxscaffold_1G00038690 Rroxscaffold_5G00349560 Rroxscaffold_5G00349610 Rroxscaffold_6G00388370 Rroxscaffold_7G00188740
rosa_rugosa Rorug03G0199700 Rorug04G0055400 Rorug04G0055500 Rorug04G0055900 Rorug04G0056000 Rorug04G0056100 Rorug04G0056100 Rorug04G0056200 Rorug05G0195600 Rorug05G0195700 Rorug05G0195800 Rorug05G0195900 Rorug05G0196800 Rorug05G0385000
rosa_samantha Rh4AG129900 Rh4AG130100 Rh4BG124200 Rh4BG124400 Rh4BG124500 Rh4CG137200 Rh4CG137300 Rh4DG123500 Rh4DG123700 Rh5AG280800 Rh5AG281400 Rh5BG286700 Rh5BG287000 Rh5BG397800 Rh5CG318100 Rh5CG318600 Rh5CG421600 Rh5DG132300 Rh5DG132400 Rh5DG295400 Rh5DG295700 Rh7BG475300
rosa_wichuraiana Rw0G001330 Rw0G005730 Rw4G008670 Rw4G010510 Rw4G010540 Rw5G026450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1746
AasI GACNNNNNNGTC 1 cut(s) 1102
Acc36I ACCTGC 2 cut(s) 3095, 3401
AccB1I GGYRCC 2 cut(s) 2906, 3331
AccB7I CCANNNNNTGG 1 cut(s) 3284
AccII CGCG 2 cut(s) 2623, 3045
AccIII TCCGGA 1 cut(s) 485
AciI CCGC 6 cut(s) 1472, 1665, 1798, 2621, 2623, 2746
AcoI YGGCCR 1 cut(s) 3154
AcsI RAATTY 5 cut(s) 101, 1019, 1207, 1423, 3403
AcuI CTGAAG 2 cut(s) 831, 1719
AcyI GRCGYC 1 cut(s) 482
AfaI GTAC 6 cut(s) 210, 1251, 1696, 1802, 1871, 1875
AflIII ACRYGT 4 cut(s) 851, 1803, 3201, 3315
AgeI ACCGGT 1 cut(s) 2480
AhlI ACTAGT 1 cut(s) 1046
AjiI CACGTC 1 cut(s) 1806
AjnI CCWGG 4 cut(s) 2257, 2595, 3326, 3340
AjuI GAANNNNNNNTTGG 2 cut(s) 1322, 1354
AleI CACNNNNGTG 2 cut(s) 1829, 1892
AloI GAACNNNNNNTCC 2 cut(s) 1168, 1200
Alw21I GWGCWC 1 cut(s) 1723
Alw26I GTCTC 7 cut(s) 1878, 1946, 2235, 2344, 2730, 2966, 3024
AlwNI CAGNNNCTG 1 cut(s) 1712
Ama87I CYCGRG 4 cut(s) 293, 350, 606, 905
Aor13HI TCCGGA 1 cut(s) 485
AoxI GGCC 3 cut(s) 2618, 3154, 3355
ApaI GGGCCC 1 cut(s) 3359
ApeKI GCWGC 9 cut(s) 5, 148, 661, 1475, 1529, 1689, 2847, 3067, 3214
ApoI RAATTY 5 cut(s) 101, 1019, 1207, 1423, 3403
ArsI GACNNNNNNTTYG 2 cut(s) 2747, 2779
AsiGI ACCGGT 1 cut(s) 2480
Asp700I GAANNNNTTC 2 cut(s) 1147, 2006
AspLEI GCGC 1 cut(s) 3047
AspS9I GGNCC 6 cut(s) 546, 1009, 2817, 2898, 3355, 3356
AsuC2I CCSGG 1 cut(s) 3375
AsuHPI GGTGA 8 cut(s) 97, 245, 1226, 1291, 2060, 2576, 3356, 3374
AsuII TTCGAA 2 cut(s) 161, 1660
AvaI CYCGRG 4 cut(s) 293, 350, 606, 905
AvaII GGWCC 4 cut(s) 546, 1009, 2817, 2898
AxyI CCTNAGG 2 cut(s) 542, 3265
BaeGI GKGCMC 2 cut(s) 3334, 3359
BalI TGGCCA 1 cut(s) 3156
BamHI GGATCC 1 cut(s) 901
BanI GGYRCC 2 cut(s) 2906, 3331
BanII GRGCYC 3 cut(s) 294, 2772, 3359
BarI GAAGNNNNNNTAC 2 cut(s) 1793, 1825
BauI CACGAG 1 cut(s) 1626
BbsI GAAGAC 6 cut(s) 715, 1049, 1070, 1096, 1337, 3021
Bbv12I GWGCWC 1 cut(s) 1723
BbvCI CCTCAGC 2 cut(s) 138, 477
BbvI GCAGC 8 cut(s) 160, 673, 1487, 1516, 1676, 2859, 3079, 3226
BceAI ACGGC 2 cut(s) 34, 586
BcgI CGANNNNNNTGC 2 cut(s) 1832, 1866
BciT130I CCWGG 4 cut(s) 2259, 2597, 3328, 3342
BciVI GTATCC 2 cut(s) 1393, 2926
BclI TGATCA 1 cut(s) 2073
BcnI CCSGG 1 cut(s) 3375
BcoDI GTCTC 7 cut(s) 1878, 1946, 2235, 2344, 2730, 2966, 3024
BcuI ACTAGT 1 cut(s) 1046
BfaI CTAG 7 cut(s) 1047, 1317, 1494, 2703, 2916, 3101, 3167
BfmI CTRYAG 4 cut(s) 1608, 1679, 1690, 2141
BfuAI ACCTGC 2 cut(s) 3095, 3401
BfuI GTATCC 2 cut(s) 1393, 2926
BmcAI AGTACT 1 cut(s) 1696
Bme1390I CCNGG 5 cut(s) 2259, 2597, 3328, 3342, 3375
Bme18I GGWCC 4 cut(s) 546, 1009, 2817, 2898
BmeT110I CYCGRG 4 cut(s) 293, 350, 606, 905
BmgBI CACGTC 1 cut(s) 1806
BmgT120I GGNCC 6 cut(s) 546, 1009, 2817, 2898, 3355, 3356
BmrFI CCNGG 5 cut(s) 2259, 2597, 3328, 3342, 3375
BmsI GCATC 9 cut(s) 527, 610, 673, 1402, 1598, 1693, 1935, 2117, 2838
BpiI GAAGAC 6 cut(s) 715, 1049, 1070, 1096, 1337, 3021
BpmI CTGGAG 2 cut(s) 377, 839
Bpu10I CCTNAGC 2 cut(s) 138, 477
Bpu14I TTCGAA 2 cut(s) 161, 1660
BpuEI CTTGAG 1 cut(s) 1967
BpuMI CCSGG 1 cut(s) 3375
Bsa29I ATCGAT 1 cut(s) 57
BsaAI YACGTR 4 cut(s) 852, 1873, 3122, 3150
BsaHI GRCGYC 1 cut(s) 482
BsaI GGTCTC 2 cut(s) 2235, 2730
BsaWI WCCGGW 4 cut(s) 485, 2056, 2480, 3399
BsaXI ACNNNNNCTCC 6 cut(s) 217, 247, 1168, 1198, 2296, 2326
Bse118I RCCGGY 2 cut(s) 35, 2480
Bse1I ACTGG 5 cut(s) 822, 2122, 2848, 2901, 2910
Bse21I CCTNAGG 2 cut(s) 542, 3265
Bse3DI GCAATG 6 cut(s) 150, 471, 630, 2557, 2701, 2857
BseAI TCCGGA 1 cut(s) 485
BseBI CCWGG 4 cut(s) 2259, 2597, 3328, 3342
BseCI ATCGAT 1 cut(s) 57
BseMI GCAATG 6 cut(s) 150, 471, 630, 2557, 2701, 2857
BseNI ACTGG 5 cut(s) 822, 2122, 2848, 2901, 2910
BseRI GAGGAG 5 cut(s) 125, 418, 421, 685, 1964
BseSI GKGCMC 2 cut(s) 3334, 3359
BseXI GCAGC 8 cut(s) 160, 673, 1487, 1516, 1676, 2859, 3079, 3226
BseYI CCCAGC 1 cut(s) 2771
BsgI GTGCAG 4 cut(s) 467, 735, 946, 1164
Bsh1236I CGCG 2 cut(s) 2623, 3045
Bsh1285I CGRYCG 2 cut(s) 1816, 2481
BshFI GGCC 3 cut(s) 2620, 3156, 3357
BshNI GGYRCC 2 cut(s) 2906, 3331
BshTI ACCGGT 1 cut(s) 2480
BshVI ATCGAT 1 cut(s) 57
BsiEI CGRYCG 2 cut(s) 1816, 2481
BsiHKAI GWGCWC 1 cut(s) 1723
BsiHKCI CYCGRG 4 cut(s) 293, 350, 606, 905
BsiSI CCGG 6 cut(s) 36, 486, 2057, 2481, 3375, 3400
BsiWI CGTACG 1 cut(s) 1869
BslFI GGGAC 3 cut(s) 1022, 1090, 2538
BsmAI GTCTC 7 cut(s) 1878, 1946, 2235, 2344, 2730, 2966, 3024
BsmFI GGGAC 3 cut(s) 1022, 1090, 2538
BsmI GAATGC 1 cut(s) 478
BsnI GGCC 3 cut(s) 2620, 3156, 3357
Bso31I GGTCTC 2 cut(s) 2235, 2730
BsoBI CYCGRG 4 cut(s) 293, 350, 606, 905
Bsp119I TTCGAA 2 cut(s) 161, 1660
Bsp120I GGGCCC 1 cut(s) 3355
Bsp1286I GDGCHC 5 cut(s) 294, 1723, 2772, 3334, 3359
Bsp13I TCCGGA 1 cut(s) 485
Bsp19I CCATGG 1 cut(s) 3003
BspACI CCGC 6 cut(s) 1472, 1665, 1798, 2621, 2623, 2746
BspANI GGCC 3 cut(s) 2620, 3156, 3357
BspDI ATCGAT 1 cut(s) 57
BspEI TCCGGA 1 cut(s) 485
BspFNI CGCG 2 cut(s) 2623, 3045
BspMAI CTGCAG 2 cut(s) 1694, 2145
BspMI ACCTGC 2 cut(s) 3095, 3401
BspQI GCTCTTC 1 cut(s) 1748
BspT104I TTCGAA 2 cut(s) 161, 1660
BspT107I GGYRCC 2 cut(s) 2906, 3331
BspTNI GGTCTC 2 cut(s) 2235, 2730
BsrDI GCAATG 6 cut(s) 150, 471, 630, 2557, 2701, 2857
BsrFI RCCGGY 2 cut(s) 35, 2480
BsrI ACTGG 5 cut(s) 822, 2122, 2848, 2901, 2910
BssAI RCCGGY 2 cut(s) 35, 2480
BssNI GRCGYC 1 cut(s) 482
BssSI CACGAG 1 cut(s) 1626
BssT1I CCWWGG 4 cut(s) 369, 2544, 3003, 3349
Bst2BI CACGAG 1 cut(s) 1626
Bst2UI CCWGG 4 cut(s) 2259, 2597, 3328, 3342
Bst6I CTCTTC 2 cut(s) 1748, 2015
BstACI GRCGYC 1 cut(s) 482
BstBAI YACGTR 4 cut(s) 852, 1873, 3122, 3150
BstBI TTCGAA 2 cut(s) 161, 1660
BstC8I GCNNGC 5 cut(s) 929, 962, 1760, 2518, 2827
BstDSI CCRYGG 3 cut(s) 558, 2621, 3003
BstEII GGTNACC 3 cut(s) 2564, 2582, 3344
BstENI CCTNNNNNAGG 1 cut(s) 949
BstFNI CGCG 2 cut(s) 2623, 3045
BstHHI GCGC 1 cut(s) 3047
BstMAI GTCTC 7 cut(s) 1878, 1946, 2235, 2344, 2730, 2966, 3024
BstMCI CGRYCG 2 cut(s) 1816, 2481
BstMWI GCNNNNNNNGC 6 cut(s) 397, 1718, 1795, 2114, 2149, 2767
BstNI CCWGG 4 cut(s) 2259, 2597, 3328, 3342
BstNSI RCATGY 3 cut(s) 1514, 3205, 3319
BstPI GGTNACC 3 cut(s) 2564, 2582, 3344
BstSCI CCNGG 5 cut(s) 2257, 2595, 3326, 3340, 3373
BstSFI CTRYAG 4 cut(s) 1608, 1679, 1690, 2141
BstSLI GKGCMC 2 cut(s) 3334, 3359
BstSNI TACGTA 1 cut(s) 1873
BstUI CGCG 2 cut(s) 2623, 3045
BstV1I GCAGC 8 cut(s) 160, 673, 1487, 1516, 1676, 2859, 3079, 3226
BstV2I GAAGAC 6 cut(s) 715, 1049, 1070, 1096, 1337, 3021
BstX2I RGATCY 7 cut(s) 190, 901, 1370, 1486, 2261, 2537, 3073
BstXI CCANNNNNNTGG 2 cut(s) 222, 3010
BstYI RGATCY 7 cut(s) 190, 901, 1370, 1486, 2261, 2537, 3073
Bsu15I ATCGAT 1 cut(s) 57
Bsu36I CCTNAGG 2 cut(s) 542, 3265
BsuI GTATCC 2 cut(s) 1393, 2926
BsuRI GGCC 3 cut(s) 2620, 3156, 3357
BsuTUI ATCGAT 1 cut(s) 57
BtgI CCRYGG 3 cut(s) 558, 2621, 3003
BtrI CACGTC 1 cut(s) 1806
BtsI GCAGTG 5 cut(s) 264, 283, 366, 455, 2869
BtsIMutI CAGTG 9 cut(s) 264, 283, 366, 455, 674, 2406, 2841, 2869, 2908
BveI ACCTGC 2 cut(s) 3095, 3401
Cac8I GCNNGC 5 cut(s) 929, 962, 1760, 2518, 2827
CaiI CAGNNNCTG 1 cut(s) 1712
CfoI GCGC 1 cut(s) 3047
Cfr10I RCCGGY 2 cut(s) 35, 2480
Cfr13I GGNCC 6 cut(s) 546, 1009, 2817, 2898, 3355, 3356
Cfr42I CCGCGG 1 cut(s) 2624
ClaI ATCGAT 1 cut(s) 57
CseI GACGC 2 cut(s) 471, 1535
CsiI ACCWGGT 2 cut(s) 2595, 3340
Csp6I GTAC 6 cut(s) 209, 1250, 1695, 1801, 1870, 1874
CspAI ACCGGT 1 cut(s) 2480
CviQI GTAC 6 cut(s) 209, 1250, 1695, 1801, 1870, 1874
DrdI GACNNNNNNGTC 1 cut(s) 1102
DseDI GACNNNNNNGTC 1 cut(s) 1102
EaeI YGGCCR 1 cut(s) 3154
Eam1104I CTCTTC 2 cut(s) 1748, 2015
EarI CTCTTC 2 cut(s) 1748, 2015
Eco105I TACGTA 1 cut(s) 1873
Eco130I CCWWGG 4 cut(s) 369, 2544, 3003, 3349
Eco24I GRGCYC 3 cut(s) 294, 2772, 3359
Eco31I GGTCTC 2 cut(s) 2235, 2730
Eco47I GGWCC 4 cut(s) 546, 1009, 2817, 2898
Eco57I CTGAAG 2 cut(s) 831, 1719
Eco81I CCTNAGG 2 cut(s) 542, 3265
Eco88I CYCGRG 4 cut(s) 293, 350, 606, 905
Eco91I GGTNACC 3 cut(s) 2564, 2582, 3344
EcoNI CCTNNNNNAGG 1 cut(s) 949
EcoO109I RGGNCCY 3 cut(s) 546, 3355, 3356
EcoO65I GGTNACC 3 cut(s) 2564, 2582, 3344
EcoRI GAATTC 3 cut(s) 101, 1207, 3403
EcoRII CCWGG 4 cut(s) 2257, 2595, 3326, 3340
EcoT14I CCWWGG 4 cut(s) 369, 2544, 3003, 3349
EcoT22I ATGCAT 2 cut(s) 1591, 2796
EcoT38I GRGCYC 3 cut(s) 294, 2772, 3359
ErhI CCWWGG 4 cut(s) 369, 2544, 3003, 3349
FaqI GGGAC 3 cut(s) 1022, 1090, 2538
FbaI TGATCA 1 cut(s) 2073
FriOI GRGCYC 3 cut(s) 294, 2772, 3359
FspBI CTAG 7 cut(s) 1047, 1317, 1494, 2703, 2916, 3101, 3167
GlaI GCGC 1 cut(s) 3046
GsaI CCCAGC 1 cut(s) 2775
GsuI CTGGAG 2 cut(s) 377, 839
HaeIII GGCC 3 cut(s) 2620, 3156, 3357
HapII CCGG 6 cut(s) 36, 486, 2057, 2481, 3375, 3400
HgaI GACGC 2 cut(s) 471, 1535
HhaI GCGC 1 cut(s) 3047
Hin1I GRCGYC 1 cut(s) 482
Hin6I GCGC 1 cut(s) 3045
HinP1I GCGC 1 cut(s) 3045
HincII GTYRAC 2 cut(s) 1033, 3181
HindII GTYRAC 2 cut(s) 1033, 3181
HindIII AAGCTT 5 cut(s) 74, 1145, 1760, 1943, 3388
HpaII CCGG 6 cut(s) 36, 486, 2057, 2481, 3375, 3400
HphI GGTGA 8 cut(s) 97, 245, 1226, 1291, 2060, 2576, 3356, 3374
Hpy166II GTNNAC 6 cut(s) 1033, 1356, 1803, 1897, 3147, 3181
Hpy8I GTNNAC 6 cut(s) 1033, 1356, 1803, 1897, 3147, 3181
HpyCH4IV ACGT 6 cut(s) 851, 1266, 1805, 1872, 3121, 3149
HpyF10VI GCNNNNNNNGC 6 cut(s) 397, 1718, 1795, 2114, 2149, 2767
HpySE526I ACGT 6 cut(s) 851, 1266, 1805, 1872, 3121, 3149
Hsp92I GRCGYC 1 cut(s) 482
HspAI GCGC 1 cut(s) 3045
Kpn2I TCCGGA 1 cut(s) 485
Ksp22I TGATCA 1 cut(s) 2073
KspI CCGCGG 1 cut(s) 2624
LguI GCTCTTC 1 cut(s) 1748
LmnI GCTCC 8 cut(s) 44, 396, 408, 698, 1726, 1866, 2163, 3376
Lsp1109I GCAGC 8 cut(s) 160, 673, 1487, 1516, 1676, 2859, 3079, 3226
LweI GCATC 9 cut(s) 527, 610, 673, 1402, 1598, 1693, 1935, 2117, 2838
MabI ACCWGGT 2 cut(s) 2595, 3340
MaeI CTAG 7 cut(s) 1047, 1317, 1494, 2703, 2916, 3101, 3167
MaeII ACGT 6 cut(s) 851, 1266, 1805, 1872, 3121, 3149
MflI RGATCY 7 cut(s) 190, 901, 1370, 1486, 2261, 2537, 3073
MhlI GDGCHC 5 cut(s) 294, 1723, 2772, 3334, 3359
MlsI TGGCCA 1 cut(s) 3156
MluNI TGGCCA 1 cut(s) 3156
MlyI GAGTC 7 cut(s) 572, 851, 1112, 2338, 2881, 3026, 3176
MmeI TCCRAC 1 cut(s) 3112
Mox20I TGGCCA 1 cut(s) 3156
Mph1103I ATGCAT 2 cut(s) 1591, 2796
MroI TCCGGA 1 cut(s) 485
MroXI GAANNNNTTC 2 cut(s) 1147, 2006
MscI TGGCCA 1 cut(s) 3156
MseI TTAA 6 cut(s) 29, 1017, 1287, 1517, 1670, 2103
MslI CAYNNNNRTG 5 cut(s) 276, 1829, 1892, 1931, 3008
Msp20I TGGCCA 1 cut(s) 3156
MspA1I CMGCKG 3 cut(s) 2623, 2746, 3176
MspI CCGG 6 cut(s) 36, 486, 2057, 2481, 3375, 3400
MspR9I CCNGG 5 cut(s) 2259, 2597, 3328, 3342, 3375
Mva1269I GAATGC 1 cut(s) 478
MvaI CCWGG 4 cut(s) 2259, 2597, 3328, 3342
MvnI CGCG 2 cut(s) 2623, 3045
MwoI GCNNNNNNNGC 6 cut(s) 397, 1718, 1795, 2114, 2149, 2767
NciI CCSGG 1 cut(s) 3375
NcoI CCATGG 1 cut(s) 3003
NmeAIII GCCGAG 2 cut(s) 426, 3208
NmuCI GTSAC 6 cut(s) 2302, 2378, 2582, 2854, 2884, 3344
NsiI ATGCAT 2 cut(s) 1591, 2796
NspI RCATGY 3 cut(s) 1514, 3205, 3319
NspV TTCGAA 2 cut(s) 161, 1660
OliI CACNNNNGTG 2 cut(s) 1829, 1892
PaeR7I CTCGAG 1 cut(s) 293
PasI CCCWGGG 1 cut(s) 3327
PciI ACATGT 2 cut(s) 3201, 3315
PciSI GCTCTTC 1 cut(s) 1748
PctI GAATGC 1 cut(s) 478
PdmI GAANNNNTTC 2 cut(s) 1147, 2006
PfeI GAWTC 8 cut(s) 119, 385, 590, 600, 859, 1729, 2710, 2777
Pfl23II CGTACG 1 cut(s) 1869
PflMI CCANNNNNTGG 1 cut(s) 3284
PfoI TCCNGGA 1 cut(s) 2257
PinAI ACCGGT 1 cut(s) 2480
PleI GAGTC 7 cut(s) 572, 850, 1111, 2337, 2881, 3026, 3176
PpsI GAGTC 7 cut(s) 572, 850, 1111, 2337, 2881, 3026, 3176
Ppu21I YACGTR 4 cut(s) 852, 1873, 3122, 3150
PpuMI RGGWCCY 1 cut(s) 546
PscI ACATGT 2 cut(s) 3201, 3315
PsiI TTATAA 1 cut(s) 1746
Psp5II RGGWCCY 1 cut(s) 546
Psp6I CCWGG 4 cut(s) 2257, 2595, 3326, 3340
PspEI GGTNACC 3 cut(s) 2564, 2582, 3344
PspFI CCCAGC 1 cut(s) 2771
PspGI CCWGG 4 cut(s) 2257, 2595, 3326, 3340
PspLI CGTACG 1 cut(s) 1869
PspOMI GGGCCC 1 cut(s) 3355
PspPI GGNCC 6 cut(s) 546, 1009, 2817, 2898, 3355, 3356
PspPPI RGGWCCY 1 cut(s) 546
PstI CTGCAG 2 cut(s) 1694, 2145
PstNI CAGNNNCTG 1 cut(s) 1712
PsuI RGATCY 7 cut(s) 190, 901, 1370, 1486, 2261, 2537, 3073
PvuII CAGCTG 1 cut(s) 3176
RsaI GTAC 6 cut(s) 210, 1251, 1696, 1802, 1871, 1875
RsaNI GTAC 6 cut(s) 209, 1250, 1695, 1801, 1870, 1874
RseI CAYNNNNRTG 5 cut(s) 276, 1829, 1892, 1931, 3008
SacII CCGCGG 1 cut(s) 2624
SapI GCTCTTC 1 cut(s) 1748
SaqAI TTAA 6 cut(s) 29, 1017, 1287, 1517, 1670, 2103
Sau96I GGNCC 6 cut(s) 546, 1009, 2817, 2898, 3355, 3356
ScaI AGTACT 1 cut(s) 1696
SchI GAGTC 7 cut(s) 572, 851, 1112, 2338, 2881, 3026, 3176
ScrFI CCNGG 5 cut(s) 2259, 2597, 3328, 3342, 3375
SduI GDGCHC 5 cut(s) 294, 1723, 2772, 3334, 3359
SexAI ACCWGGT 2 cut(s) 2595, 3340
SfaNI GCATC 9 cut(s) 527, 610, 673, 1402, 1598, 1693, 1935, 2117, 2838
SfcI CTRYAG 4 cut(s) 1608, 1679, 1690, 2141
Sfr274I CTCGAG 1 cut(s) 293
Sfr303I CCGCGG 1 cut(s) 2624
SfuI TTCGAA 2 cut(s) 161, 1660
SgrBI CCGCGG 1 cut(s) 2624
SinI GGWCC 4 cut(s) 546, 1009, 2817, 2898
SlaI CTCGAG 1 cut(s) 293
SmiMI CAYNNNNRTG 5 cut(s) 276, 1829, 1892, 1931, 3008
SmlI CTYRAG 2 cut(s) 293, 1946
SmoI CTYRAG 2 cut(s) 293, 1946
SnaBI TACGTA 1 cut(s) 1873
SpeI ACTAGT 1 cut(s) 1046
SsiI CCGC 6 cut(s) 1472, 1665, 1798, 2621, 2623, 2746
SspMI CTAG 7 cut(s) 1047, 1317, 1494, 2703, 2916, 3101, 3167
StyD4I CCNGG 5 cut(s) 2257, 2595, 3326, 3340, 3373
StyI CCWWGG 4 cut(s) 369, 2544, 3003, 3349
TaiI ACGT 6 cut(s) 854, 1269, 1808, 1875, 3124, 3152
TaqI TCGA 7 cut(s) 57, 161, 294, 338, 1133, 1660, 1842
TaqII GACCGA 1 cut(s) 1802
TatI WGTACW 2 cut(s) 208, 1694
TauI GCSGC 2 cut(s) 1475, 2623
TfiI GAWTC 8 cut(s) 119, 385, 590, 600, 859, 1729, 2710, 2777
Tru1I TTAA 6 cut(s) 29, 1017, 1287, 1517, 1670, 2103
Tru9I TTAA 6 cut(s) 29, 1017, 1287, 1517, 1670, 2103
TscAI CASTG 9 cut(s) 264, 283, 366, 455, 674, 2406, 2848, 2869, 2908
TseFI GTSAC 6 cut(s) 2302, 2378, 2582, 2854, 2884, 3344
TseI GCWGC 9 cut(s) 5, 148, 661, 1475, 1529, 1689, 2847, 3067, 3214
Tsp45I GTSAC 6 cut(s) 2302, 2378, 2582, 2854, 2884, 3344
TspGWI ACGGA 1 cut(s) 1050
TspRI CASTG 9 cut(s) 264, 283, 366, 455, 674, 2406, 2848, 2869, 2908
Van91I CCANNNNNTGG 1 cut(s) 3284
VpaK11BI GGWCC 4 cut(s) 546, 1009, 2817, 2898
XagI CCTNNNNNAGG 1 cut(s) 949
XapI RAATTY 5 cut(s) 101, 1019, 1207, 1423, 3403
XceI RCATGY 3 cut(s) 1514, 3205, 3319
XcmI CCANNNNNNNNNTGG 3 cut(s) 2322, 2576, 3281
XhoI CTCGAG 1 cut(s) 293
XmnI GAANNNNTTC 2 cut(s) 1147, 2006
XspI CTAG 7 cut(s) 1047, 1317, 1494, 2703, 2916, 3101, 3167
ZrmI AGTACT 1 cut(s) 1696
Zsp2I ATGCAT 2 cut(s) 1591, 2796
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.