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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 1 cut(s) | 73 |
| AfaI | GTAC | 2 cut(s) | 15, 175 |
| AgsI | TTSAA | 3 cut(s) | 73, 125, 155 |
| AlwI | GGATC | 1 cut(s) | 73 |
| BfaI | CTAG | 1 cut(s) | 18 |
| BpuEI | CTTGAG | 1 cut(s) | 137 |
| Bsa29I | ATCGAT | 1 cut(s) | 45 |
| BseCI | ATCGAT | 1 cut(s) | 45 |
| BshVI | ATCGAT | 1 cut(s) | 45 |
| Bsp143I | GATC | 1 cut(s) | 65 |
| BspDI | ATCGAT | 1 cut(s) | 45 |
| BspPI | GGATC | 1 cut(s) | 73 |
| BssMI | GATC | 1 cut(s) | 65 |
| BstKTI | GATC | 1 cut(s) | 68 |
| BstMBI | GATC | 1 cut(s) | 65 |
| BstX2I | RGATCY | 1 cut(s) | 65 |
| BstYI | RGATCY | 1 cut(s) | 65 |
| Bsu15I | ATCGAT | 1 cut(s) | 45 |
| BsuTUI | ATCGAT | 1 cut(s) | 45 |
| ClaI | ATCGAT | 1 cut(s) | 45 |
| Csp6I | GTAC | 2 cut(s) | 14, 174 |
| CviQI | GTAC | 2 cut(s) | 14, 174 |
| DpnI | GATC | 1 cut(s) | 67 |
| DpnII | GATC | 1 cut(s) | 65 |
| FaiI | YATR | 4 cut(s) | 135, 147, 149, 184 |
| FauNDI | CATATG | 1 cut(s) | 147 |
| FspBI | CTAG | 1 cut(s) | 18 |
| FspEI | CC | 8 cut(s) | 30, 36, 47, 48, 82, 90, 120, 143 |
| HinfI | GANTC | 1 cut(s) | 121 |
| Hpy166II | GTNNAC | 1 cut(s) | 174 |
| Hpy188I | TCNGA | 1 cut(s) | 79 |
| Hpy8I | GTNNAC | 1 cut(s) | 174 |
| HpyCH4V | TGCA | 1 cut(s) | 145 |
| Kzo9I | GATC | 1 cut(s) | 65 |
| MaeI | CTAG | 1 cut(s) | 18 |
| MalI | GATC | 1 cut(s) | 67 |
| MboI | GATC | 1 cut(s) | 65 |
| MfeI | CAATTG | 1 cut(s) | 125 |
| MflI | RGATCY | 1 cut(s) | 65 |
| MluCI | AATT | 2 cut(s) | 110, 125 |
| MnlI | CCTC | 1 cut(s) | 98 |
| MunI | CAATTG | 1 cut(s) | 125 |
| NdeI | CATATG | 1 cut(s) | 147 |
| NdeII | GATC | 1 cut(s) | 65 |
| PfeI | GAWTC | 1 cut(s) | 121 |
| PsuI | RGATCY | 1 cut(s) | 65 |
| RsaI | GTAC | 2 cut(s) | 15, 175 |
| RsaNI | GTAC | 2 cut(s) | 14, 174 |
| Sau3AI | GATC | 1 cut(s) | 65 |
| SetI | ASST | 2 cut(s) | 19, 101 |
| SgeI | CNNG | 5 cut(s) | 30, 42, 116, 128, 176 |
| SmlI | CTYRAG | 1 cut(s) | 116 |
| SmoI | CTYRAG | 1 cut(s) | 116 |
| Sse9I | AATT | 2 cut(s) | 110, 125 |
| SspMI | CTAG | 1 cut(s) | 18 |
| TaqI | TCGA | 3 cut(s) | 45, 103, 165 |
| TasI | AATT | 2 cut(s) | 110, 125 |
| TfiI | GAWTC | 1 cut(s) | 121 |
| XspI | CTAG | 1 cut(s) | 18 |