RchiOBHm_Chr4g0405271

metal ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000343
Physical Location & Seq
Forward (+)
25487388 .. 25492978
5591 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37676

Sequence Viewer

Length: 2892 bp
ATGGCATCGGCAGAGTGTCAACCATATTCTGTTTCGGAGTACCATTTCGTAGATGACAAGGATGAATGTGTCTCATTTCATGTATTGCCTGTTCAATGGAGTGATGGTGAGAGACTGGATGGTGAGAAAGTAGAGATTTTTCTGCATGGAACCGCTGATAATGGGGGGAATTTATGTAAGCATGTTGTTGCATGGAAGTTTGACATTTCGAGTGAAGAGCCCGAGATATTGGTTCTCTCTGAGGAAAATAGTTGGATCAAGCTTGGGAAGCCGAGGAAGAGCTTTGAGGACACTGTTAGGTCGATCTTGATAACGGTGCACTGCCTTCACTATGTGAAGAGAAGTCCTGAAAAATCCAGAGAATGTGTGTGGAATCACCTATCTGAAGTTTTCAGGTTGTATGTGGTCAGGCCTTCTCAAAGTGATCTGGTGGATCACATGCCTCTAATCAGTGATTGTGTTAACAGATATCGTGCCCTAGCGGATTCCAAGTTTTTGGCCAAACTTTTTGAAGAGAAGACAATGAAGAGGAAACTCTCTGATGAGGATACTGAAGCTAGAAAGAAGCTCAGAATTATGATCGATGATGTAAAAGATGATATGATTCATGCAGAAGATGAGGATATCGATAACCTGTTTGGTGACGTCTGTGCTTTATGTGACGATGGTGGCAAGATCATTTGTTGTGAAGGGGGGTGCCTAAGGTCTTTTCATGCAACTGAGGAGGCGGGTGCCGGATCTTATTGCAAATCTCTTGGCTTTATGCAGGATTATGTTGTTGGGATGCAAAATTTCTACTGCAAGAACTGTGAATATAAACAGCACCAGTGCTTTGTTTGTGGGAAGTTAGGTTCCTCTGATAAGTCCTCAGGTGCTGCTGAGGTGGTTTCCTGTGTATCCCCAAACTGTGGTCGATTTTACCATCCACATTGTGTTGCAATATTACTTTACCAAGATAATGGAGTTCCTGCTGAAGAACTTGAGAGAAAGATTGCTGGTGGGGAATCTTTTACTTGTCCACTTCATAAGTGCTGCATTTGTAAACAAGGAGAAAATAAGAAGGATAGTGAGTTACAATTTGCTGTGTGCATGCGTTGTCCAAAATCGTACCACAGGAAATGCCTGCCAAGGGAGATTGCTTTTGAAAATCAAGGAGAAATGCTAGAAGGGAGATCAATAATAAGAGCTTGGGATGGTCTATTACCTAACCGTATACTCATATATTGCACAAAACATGATATAGATATTGAATTTGGAACTGTAAGAAGGGACCACGTAAAATTCCCTGATGTCAAACAGAATATGAGCACTCTCACAAAGAAGAAGAAGAAGAAGAAGACTATTTTAGAGGAGAAGTGGAAGCCAATATCAGAATTCCTTGTGTACGGAGACAAAGTTGTGACTAAGAGAAATATTTCCTTGGAAAAATCATGCAGGGATAAAAATGCTCCTACAGCACTTAAGACACAAAAGCCATATGTTCAAGAGAAGAAGACTAGGAAACTTATGTCAGAGCAGAAACCTGTGTCTAAGAAGAGAGATTCTTCTTTGGAAGAATCCGTTGGGGAAGGAAGTGATCCTGTACTATCCACACTAGTCAAGCAGAAGCTTTATTCAGTTAAAGAGAAGAAGGCTACTTTTGTAAAAGAGAAAACTTTTGTTGAAGAGAAAGAAAGCCAACTGACATCAACGGTCTCTGTAGACAGAGAAAAAGCTGTGTTGAAGAGAACTCCTTCCTCTGAAGGACTATATAGTGAGAAAAGTGCTCGTACAATATCCAAGAAAAAACAGAAGCCATCTTTTGTTGAAGAGAAGAACAATGACCTTGTAAAAAAGAAAACTGATGTTGAAGAGAAGAGGAAACAGACATCAGAGTCCCTTGTTGAGAAAGAGACAGCTGCGTCCAAAAAGAAAAATCCTTCCTCTAAAGAATCCTATAGGGAAAGAAATGCTTCTACAACTCCCAAACAGAAGCAGAAGCCATCAGAGCTCACTGTAGATGGAGATGAGGCTGGGTCTAAGAAGGGAAGTCCTTTGGAAGAATCATTAAGTGCAATTGCTTCAACTGTACTGAAGCAGAAGTCGACTTCTGCCGTGAAGCTGGGTAGTAACAAGAATAGGGAAAAGACGTTTTCTGGTATAGATATTTCAAGGATGGTGAAACGAAATAATTCACTGAAAAATGAACGTCAGGCTTCCATGTCTGAGAAAATCAAGACCTCACTGGGTGAAGAGCCATTTTCTTCAATGGAGTCTGAGCAGGTGAAGCTCGGGAACCTGGATACACCTGATGCTAATACAGTTAGTGAGATACACAGGGAAATGCCACCACATACTGTATGTGGTGACGATGGAAATCAGCATCCTAACCCCAGCAAGTCTCGTATGCACTGTGCTCATGCTAACGGTCGAGTCCAATTCAAAATTCCTGATGATGCAGGCAGAAGGTGCACCACGAGTAATGATGAGCCTCGTTCGAGTGTTACCAATCCTAGGTCTGGCAATGCGAAAGGGTGCAAGAGGGAGAACTCGAGAAAACCATATTCAAATGGGACGAAACAGAAACAGAAACTGAGAACAGAATTTGACAGAGTCCCACCGGCTTGCATTAAAGGGAAGCAGGATCCTATTTTTTCAAGAGGCAATGATCTAGCGGGCCAAAATTCAGTATATGACCATATTAGGACGTTCTCATCTACTTTTTACAGCCATACAGGTCCTGTTGCTGACTCGTTATCTACTATCAACAGATTAGCTATGCCATCAGATGCAACTAAATTGGTTGGTCCAAGAGCACCCCAACCTGATTACCTGGATAGGTGTATGGGTTTCGCTCCTGGTCCGCACCTGAACTATTCACTACAGAATTCAGCTGGTTGGATCGATGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006325 GO:0006355 GO:0006950 GO:0006952 GO:0006996 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0009605 GO:0009607 GO:0009620 GO:0009791 GO:0009889 GO:0009893 GO:0009909 GO:0009911 GO:0009987 GO:0010228 GO:0010468 GO:0010556 GO:0010604 GO:0010638 GO:0016043 GO:0019219 GO:0019222 GO:0022414 GO:0023052 GO:0030154 GO:0031056 GO:0031058 GO:0031060 GO:0031062 GO:0031323 GO:0031325 GO:0031326 GO:0031399 GO:0031401 GO:0031440 GO:0031935 GO:0031937 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033043 GO:0033044 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0043954 GO:0044030 GO:0044424 GO:0044464 GO:0048366 GO:0048367 GO:0048468 GO:0048518 GO:0048522 GO:0048580 GO:0048582 GO:0048608 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0048869 GO:0050684 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051052 GO:0051094 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051276 GO:0051570 GO:0051574 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060968 GO:0061085 GO:0061087 GO:0061458 GO:0065007 GO:0070827 GO:0070828 GO:0070829 GO:0071840 GO:0080090 GO:0090436 GO:0097159 GO:0098542 GO:0099402 GO:0140110 GO:1900109 GO:1900111 GO:1900363 GO:1901363 GO:1902275 GO:1903311 GO:1903506 GO:1905269 GO:2000024 GO:2000026 GO:2000112 GO:2000241 GO:2000243 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNMT1-RFD

Protein Analysis

963

Amino Acids

108.41

Weight (kDa)

8.75

Isoelectric Point (pI)

49.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNMT1-RFD PF12047 11 - 132 3.4e-10 Cytosine specific DNA methyltransferase replication foci domain
PHD_NSD PF22908 273 - 342 3.4e-19 Histone-lysine N-methyltransferase NSD-like, PHD zinc finger
PHDvar_NSD PF23004 344 - 380 6e-06 Histone-lysine N-methyltransferase NSD-like, variant PHD zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000343)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48090 AT5G48090 AT5G48090 AT5G55390 AT5G55390 AT5G55390
fragaria_vesca FvH4_3g23690 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160
malus_domestica MD03G1191000.v1.1 MD11G1207800.v1.1 MD11G1208000.v1.1
prunus_persica Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227600_v2.0.a1 Prupe.4G227700_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1
pyrus_communis pycom01g03400 pycom03g14420 pycom11g18100 pycom11g18140 pycom11g18150 pycom11g18160
rosa_chinensis RchiOBHm_Chr2g0145981 RchiOBHm_Chr4g0405241 RchiOBHm_Chr4g0405271 RchiOBHm_Chr5g0016201 RchiOBHm_Chr5g0041831 RchiOBHm_Chr5g0041871 RchiOBHm_Chr5g0041881 RchiOBHm_Chr5g0059241
rosa_laevigata RLG00000002117 RLG00000008861 RLG00000008862 RLG00000011000 RLG00000033216 RLG00000034097 RLG00000034101
rosa_multiflora Rmu_sc0000161.1_g000001 Rmu_sc0000861.1_g000040 Rmu_sc0007522.1_g000003 Rmu_sc0009443.1_g000015 Rmu_sc0022129.1_g000001 Rmu_sc0042315.1_g000001
rosa_roxburghii Rroxscaffold_1G00038660 Rroxscaffold_1G00038690 Rroxscaffold_5G00349560 Rroxscaffold_5G00349610 Rroxscaffold_6G00388370 Rroxscaffold_7G00188740
rosa_rugosa Rorug03G0199700 Rorug04G0055400 Rorug04G0055500 Rorug04G0055900 Rorug04G0056000 Rorug04G0056100 Rorug04G0056100 Rorug04G0056200 Rorug05G0195600 Rorug05G0195700 Rorug05G0195800 Rorug05G0195900 Rorug05G0196800 Rorug05G0385000
rosa_samantha Rh4AG129900 Rh4AG130100 Rh4BG124200 Rh4BG124400 Rh4BG124500 Rh4CG137200 Rh4CG137300 Rh4DG123500 Rh4DG123700 Rh5AG280800 Rh5AG281400 Rh5BG286700 Rh5BG287000 Rh5BG397800 Rh5CG318100 Rh5CG318600 Rh5CG421600 Rh5DG132300 Rh5DG132400 Rh5DG295400 Rh5DG295700 Rh7BG475300
rosa_wichuraiana Rw0G001330 Rw0G005730 Rw4G008670 Rw4G010510 Rw4G010540 Rw5G026450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2251
AasI GACNNNNNNGTC 2 cut(s) 1873, 1900
AatII GACGTC 1 cut(s) 648
Acc36I ACCTGC 1 cut(s) 2251
AccB1I GGYRCC 2 cut(s) 696, 731
AccB7I CCANNNNNTGG 1 cut(s) 908
AccI GTMKAC 3 cut(s) 1213, 1701, 2084
AciI CCGC 5 cut(s) 153, 482, 728, 2654, 2843
AclWI GGATC 7 cut(s) 263, 441, 745, 1571, 2618, 2631, 2888
AcoI YGGCCR 1 cut(s) 498
AcsI RAATTY 9 cut(s) 169, 790, 1250, 1280, 1373, 2424, 2582, 2662, 2866
AcuI CTGAAG 5 cut(s) 405, 573, 993, 1761, 2093
AcyI GRCGYC 1 cut(s) 645
AdeI CACNNNGTG 3 cut(s) 334, 932, 2228
AfaI GTAC 6 cut(s) 41, 1109, 1385, 1584, 1771, 2070
AfiI CCNNNNNNNGG 4 cut(s) 908, 1129, 2493, 2498
AflII CTTAAG 1 cut(s) 1460
AhlI ACTAGT 1 cut(s) 1594
AjnI CCWGG 3 cut(s) 2277, 2811, 2836
AjuI GAANNNNNNNTTGG 4 cut(s) 1545, 1577, 2409, 2441
AloI GAACNNNNNNTCC 2 cut(s) 835, 867
Alw21I GWGCWC 7 cut(s) 321, 1310, 1768, 1992, 2398, 2453, 2797
Alw26I GTCTC 6 cut(s) 76, 106, 1383, 1699, 1886, 2385
Alw44I GTGCAC 2 cut(s) 317, 2449
AlwI GGATC 7 cut(s) 263, 441, 745, 1571, 2618, 2631, 2888
AlwNI CAGNNNCTG 3 cut(s) 875, 2572, 2720
Ama87I CYCGRG 3 cut(s) 221, 2270, 2530
AoxI GGCC 3 cut(s) 410, 498, 2656
ApaLI GTGCAC 2 cut(s) 317, 2449
ApeKI GCWGC 3 cut(s) 875, 1032, 1898
ApoI RAATTY 9 cut(s) 169, 790, 1250, 1280, 1373, 2424, 2582, 2662, 2866
Asp700I GAANNNNTTC 4 cut(s) 1414, 1732, 1951, 2170
AspA2I CCTAGG 1 cut(s) 2492
AspS9I GGNCC 5 cut(s) 1270, 2656, 2717, 2786, 2840
AsuHPI GGTGA 8 cut(s) 119, 134, 368, 653, 2170, 2240, 2275, 2357
AvaI CYCGRG 3 cut(s) 221, 2270, 2530
AvaII GGWCC 4 cut(s) 1270, 2717, 2786, 2840
AvrII CCTAGG 1 cut(s) 2492
AxyI CCTNAGG 2 cut(s) 701, 868
BaeGI GKGCMC 3 cut(s) 321, 478, 2453
BalI TGGCCA 1 cut(s) 500
BamHI GGATCC 1 cut(s) 2623
BanI GGYRCC 2 cut(s) 696, 731
BanII GRGCYC 2 cut(s) 222, 1992
BauI CACGAG 1 cut(s) 2455
BbsI GAAGAC 3 cut(s) 524, 1343, 1499
Bbv12I GWGCWC 7 cut(s) 321, 1310, 1768, 1992, 2398, 2453, 2797
BbvCI CCTCAGC 1 cut(s) 879
BbvI GCAGC 3 cut(s) 862, 1019, 1885
BceAI ACGGC 1 cut(s) 2078
BciT130I CCWGG 3 cut(s) 2279, 2813, 2838
BciVI GTATCC 3 cut(s) 541, 907, 2275
BcoDI GTCTC 6 cut(s) 76, 106, 1383, 1699, 1886, 2385
BcuI ACTAGT 1 cut(s) 1594
BfaI CTAG 7 cut(s) 479, 558, 1163, 1497, 1595, 2493, 2651
BfmI CTRYAG 5 cut(s) 1452, 1698, 1936, 1995, 2861
BfrI CTTAAG 1 cut(s) 1460
BfuAI ACCTGC 1 cut(s) 2251
BfuI GTATCC 3 cut(s) 541, 907, 2275
BisI GCNGC 3 cut(s) 876, 1033, 1899
BlnI CCTAGG 1 cut(s) 2492
BlsI GCNGC 3 cut(s) 877, 1034, 1900
Bme1390I CCNGG 3 cut(s) 2279, 2813, 2838
Bme18I GGWCC 4 cut(s) 1270, 2717, 2786, 2840
BmeT110I CYCGRG 3 cut(s) 221, 2270, 2530
BmgT120I GGNCC 5 cut(s) 1270, 2656, 2717, 2786, 2840
BmiI GGNNCC 7 cut(s) 151, 698, 733, 853, 1271, 2276, 2625
BmrFI CCNGG 3 cut(s) 2279, 2813, 2838
BmrI ACTGGG 1 cut(s) 2234
BmsI GCATC 6 cut(s) 14, 774, 2281, 2371, 2425, 2758
BmuI ACTGGG 1 cut(s) 2234
BpiI GAAGAC 3 cut(s) 524, 1343, 1499
Bpu10I CCTNAGC 1 cut(s) 879
BpuEI CTTGAG 1 cut(s) 1001
Bsa29I ATCGAT 3 cut(s) 582, 627, 2883
BsaAI YACGTR 1 cut(s) 1276
BsaBI GATNNNNATC 1 cut(s) 2355
BsaHI GRCGYC 1 cut(s) 645
BsaI GGTCTC 1 cut(s) 1699
BsaJI CCNNGG 4 cut(s) 272, 1127, 1419, 2492
BsaXI ACNNNNNCTCC 4 cut(s) 91, 121, 1380, 1410
Bsc4I CCNNNNNNNGG 4 cut(s) 908, 1129, 2493, 2498
Bse118I RCCGGY 1 cut(s) 2599
Bse1I ACTGG 3 cut(s) 120, 826, 2229
Bse21I CCTNAGG 2 cut(s) 701, 868
Bse3DI GCAATG 2 cut(s) 2509, 2650
Bse8I GATNNNNATC 1 cut(s) 2355
BseBI CCWGG 3 cut(s) 2279, 2813, 2838
BseCI ATCGAT 3 cut(s) 582, 627, 2883
BseDI CCNNGG 4 cut(s) 272, 1127, 1419, 2492
BseGI GGATG 7 cut(s) 67, 124, 789, 922, 1198, 2160, 2362
BseJI GATNNNNATC 1 cut(s) 2355
BseLI CCNNNNNNNGG 4 cut(s) 908, 1129, 2493, 2498
BseMI GCAATG 2 cut(s) 2509, 2650
BseMII CTCAG 8 cut(s) 231, 583, 711, 870, 882, 2196, 2247, 2564
BseNI ACTGG 3 cut(s) 120, 826, 2229
BseRI GAGGAG 2 cut(s) 737, 1364
BseSI GKGCMC 3 cut(s) 321, 478, 2453
BseXI GCAGC 3 cut(s) 862, 1019, 1885
BseYI CCCAGC 3 cut(s) 2012, 2101, 2372
Bsh1285I CGRYCG 1 cut(s) 2410
BshFI GGCC 3 cut(s) 412, 500, 2658
BshNI GGYRCC 2 cut(s) 696, 731
BshVI ATCGAT 3 cut(s) 582, 627, 2883
BsiEI CGRYCG 1 cut(s) 2410
BsiHKAI GWGCWC 7 cut(s) 321, 1310, 1768, 1992, 2398, 2453, 2797
BsiHKCI CYCGRG 3 cut(s) 221, 2270, 2530
BsiSI CCGG 2 cut(s) 735, 2600
BslFI GGGAC 4 cut(s) 1283, 1861, 2566, 2579
BslI CCNNNNNNNGG 4 cut(s) 908, 1129, 2493, 2498
BsmAI GTCTC 6 cut(s) 76, 106, 1383, 1699, 1886, 2385
BsmFI GGGAC 4 cut(s) 1283, 1861, 2566, 2579
BsnI GGCC 3 cut(s) 412, 500, 2658
Bso31I GGTCTC 1 cut(s) 1699
BsoBI CYCGRG 3 cut(s) 221, 2270, 2530
Bsp1286I GDGCHC 9 cut(s) 222, 321, 478, 1310, 1768, 1992, 2398, 2453, 2797
BspACI CCGC 5 cut(s) 153, 482, 728, 2654, 2843
BspANI GGCC 3 cut(s) 412, 500, 2658
BspCNI CTCAG 8 cut(s) 232, 582, 712, 871, 881, 2197, 2248, 2565
BspDI ATCGAT 3 cut(s) 582, 627, 2883
BspLI GGNNCC 7 cut(s) 151, 698, 733, 853, 1271, 2276, 2625
BspMI ACCTGC 1 cut(s) 2251
BspPI GGATC 7 cut(s) 263, 441, 745, 1571, 2618, 2631, 2888
BspQI GCTCTTC 3 cut(s) 210, 272, 2226
BspT107I GGYRCC 2 cut(s) 696, 731
BspTI CTTAAG 1 cut(s) 1460
BspTNI GGTCTC 1 cut(s) 1699
BsrDI GCAATG 2 cut(s) 2509, 2650
BsrFI RCCGGY 1 cut(s) 2599
BsrI ACTGG 3 cut(s) 120, 826, 2229
BssAI RCCGGY 1 cut(s) 2599
BssECI CCNNGG 4 cut(s) 272, 1127, 1419, 2492
BssNAI GTATAC 1 cut(s) 1214
BssNI GRCGYC 1 cut(s) 645
BssSI CACGAG 1 cut(s) 2455
BssT1I CCWWGG 3 cut(s) 1127, 1419, 2492
Bst1107I GTATAC 1 cut(s) 1214
Bst2BI CACGAG 1 cut(s) 2455
Bst2UI CCWGG 3 cut(s) 2279, 2813, 2838
BstACI GRCGYC 1 cut(s) 645
BstAFI CTTAAG 1 cut(s) 1460
BstAPI GCANNNNNTGC 1 cut(s) 2448
BstBAI YACGTR 1 cut(s) 1276
BstC8I GCNNGC 5 cut(s) 1091, 1124, 2440, 2605, 2656
BstF5I GGATG 7 cut(s) 67, 124, 789, 922, 1198, 2160, 2362
BstMAI GTCTC 6 cut(s) 76, 106, 1383, 1699, 1886, 2385
BstMCI CGRYCG 1 cut(s) 2410
BstMWI GCNNNNNNNGC 5 cut(s) 268, 1454, 1987, 2266, 2448
BstNI CCWGG 3 cut(s) 2279, 2813, 2838
BstNSI RCATGY 3 cut(s) 185, 442, 1093
BstSCI CCNGG 3 cut(s) 2277, 2811, 2836
BstSFI CTRYAG 5 cut(s) 1452, 1698, 1936, 1995, 2861
BstSLI GKGCMC 3 cut(s) 321, 478, 2453
BstV1I GCAGC 3 cut(s) 862, 1019, 1885
BstV2I GAAGAC 3 cut(s) 524, 1343, 1499
BstX2I RGATCY 2 cut(s) 737, 2623
BstXI CCANNNNNNTGG 2 cut(s) 496, 959
BstYI RGATCY 2 cut(s) 737, 2623
BstZ17I GTATAC 1 cut(s) 1214
Bsu15I ATCGAT 3 cut(s) 582, 627, 2883
Bsu36I CCTNAGG 2 cut(s) 701, 868
BsuI GTATCC 3 cut(s) 541, 907, 2275
BsuRI GGCC 3 cut(s) 412, 500, 2658
BsuTUI ATCGAT 3 cut(s) 582, 627, 2883
BtsCI GGATG 7 cut(s) 67, 124, 789, 922, 1198, 2160, 2362
BtsI GCAGTG 1 cut(s) 319
BtsIMutI CAGTG 8 cut(s) 291, 319, 457, 833, 1992, 2174, 2222, 2389
BveI ACCTGC 1 cut(s) 2251
Cac8I GCNNGC 5 cut(s) 1091, 1124, 2440, 2605, 2656
CaiI CAGNNNCTG 3 cut(s) 875, 2572, 2720
Cfr10I RCCGGY 1 cut(s) 2599
Cfr13I GGNCC 5 cut(s) 1270, 2656, 2717, 2786, 2840
ClaI ATCGAT 3 cut(s) 582, 627, 2883
CseI GACGC 1 cut(s) 1890
Csp6I GTAC 6 cut(s) 40, 1108, 1384, 1583, 1770, 2069
CviQI GTAC 6 cut(s) 40, 1108, 1384, 1583, 1770, 2069
DraIII CACNNNGTG 3 cut(s) 334, 932, 2228
DrdI GACNNNNNNGTC 2 cut(s) 1873, 1900
DseDI GACNNNNNNGTC 2 cut(s) 1873, 1900
EaeI YGGCCR 1 cut(s) 498
Ecl136II GAGCTC 1 cut(s) 1990
Eco130I CCWWGG 3 cut(s) 1127, 1419, 2492
Eco147I AGGCCT 1 cut(s) 412
Eco24I GRGCYC 2 cut(s) 222, 1992
Eco31I GGTCTC 1 cut(s) 1699
Eco32I GATATC 2 cut(s) 470, 625
Eco47I GGWCC 4 cut(s) 1270, 2717, 2786, 2840
Eco53kI GAGCTC 1 cut(s) 1990
Eco57I CTGAAG 5 cut(s) 405, 573, 993, 1761, 2093
Eco81I CCTNAGG 2 cut(s) 701, 868
Eco88I CYCGRG 3 cut(s) 221, 2270, 2530
EcoICRI GAGCTC 1 cut(s) 1990
EcoO109I RGGNCCY 1 cut(s) 2717
EcoRI GAATTC 2 cut(s) 1373, 2866
EcoRII CCWGG 3 cut(s) 2277, 2811, 2836
EcoRV GATATC 2 cut(s) 470, 625
EcoT14I CCWWGG 3 cut(s) 1127, 1419, 2492
EcoT38I GRGCYC 2 cut(s) 222, 1992
ErhI CCWWGG 3 cut(s) 1127, 1419, 2492
FalI AAGNNNNNCTT 2 cut(s) 1936, 1968
FaqI GGGAC 4 cut(s) 1283, 1861, 2566, 2579
FauI CCCGC 2 cut(s) 721, 2647
FauNDI CATATG 1 cut(s) 1477
FblI GTMKAC 3 cut(s) 1213, 1701, 2084
Fnu4HI GCNGC 3 cut(s) 876, 1033, 1899
FokI GGATG 7 cut(s) 74, 131, 796, 909, 1205, 2167, 2349
FriOI GRGCYC 2 cut(s) 222, 1992
Fsp4HI GCNGC 3 cut(s) 876, 1033, 1899
FspBI CTAG 7 cut(s) 479, 558, 1163, 1497, 1595, 2493, 2651
GluI GCNGC 3 cut(s) 876, 1033, 1899
GsaI CCCAGC 3 cut(s) 2016, 2105, 2376
HaeIII GGCC 3 cut(s) 412, 500, 2658
HapII CCGG 2 cut(s) 735, 2600
HgaI GACGC 1 cut(s) 1890
Hin1I GRCGYC 1 cut(s) 645
HincII GTYRAC 3 cut(s) 20, 463, 2085
HindII GTYRAC 3 cut(s) 20, 463, 2085
HindIII AAGCTT 2 cut(s) 260, 1607
HpaI GTTAAC 1 cut(s) 463
HpaII CCGG 2 cut(s) 735, 2600
HphI GGTGA 8 cut(s) 119, 134, 368, 653, 2170, 2240, 2275, 2357
Hpy188III TCNNGA 9 cut(s) 307, 347, 357, 1484, 2215, 2272, 2429, 2532, 2637
HpyCH4IV ACGT 5 cut(s) 645, 1275, 2129, 2188, 2687
HpyF10VI GCNNNNNNNGC 5 cut(s) 268, 1454, 1987, 2266, 2448
HpySE526I ACGT 5 cut(s) 645, 1275, 2129, 2188, 2687
Hsp92I GRCGYC 1 cut(s) 645
KspAI GTTAAC 1 cut(s) 463
LguI GCTCTTC 3 cut(s) 210, 272, 2226
LmnI GCTCC 2 cut(s) 1453, 2839
Lsp1109I GCAGC 3 cut(s) 862, 1019, 1885
LweI GCATC 6 cut(s) 14, 774, 2281, 2371, 2425, 2758
MaeI CTAG 7 cut(s) 479, 558, 1163, 1497, 1595, 2493, 2651
MaeII ACGT 5 cut(s) 645, 1275, 2129, 2188, 2687
MaeIII GTNAC 7 cut(s) 641, 659, 1071, 1399, 2108, 2345, 2482
MfeI CAATTG 1 cut(s) 2055
MflI RGATCY 2 cut(s) 737, 2623
MhlI GDGCHC 9 cut(s) 222, 321, 478, 1310, 1768, 1992, 2398, 2453, 2797
MlsI TGGCCA 1 cut(s) 500
MluNI TGGCCA 1 cut(s) 500
MlyI GAGTC 5 cut(s) 1883, 2261, 2421, 2601, 2723
MmeI TCCRAC 2 cut(s) 233, 2858
Mox20I TGGCCA 1 cut(s) 500
MroXI GAANNNNTTC 4 cut(s) 1414, 1732, 1951, 2170
MscI TGGCCA 1 cut(s) 500
MseI TTAA 5 cut(s) 462, 1461, 1620, 2048, 2610
Msp20I TGGCCA 1 cut(s) 500
MspA1I CMGCKG 3 cut(s) 155, 1898, 2873
MspCI CTTAAG 1 cut(s) 1460
MspI CCGG 2 cut(s) 735, 2600
MspR9I CCNGG 3 cut(s) 2279, 2813, 2838
MunI CAATTG 1 cut(s) 2055
MvaI CCWGG 3 cut(s) 2279, 2813, 2838
MwoI GCNNNNNNNGC 5 cut(s) 268, 1454, 1987, 2266, 2448
NdeI CATATG 1 cut(s) 1477
NlaIV GGNNCC 7 cut(s) 151, 698, 733, 853, 1271, 2276, 2625
NmeAIII GCCGAG 1 cut(s) 297
NmuCI GTSAC 4 cut(s) 641, 659, 1399, 2345
NspI RCATGY 3 cut(s) 185, 442, 1093
PaeI GCATGC 1 cut(s) 1093
PaeR7I CTCGAG 1 cut(s) 2530
PaqCI CACCTGC 1 cut(s) 2251
PceI AGGCCT 1 cut(s) 412
PciSI GCTCTTC 3 cut(s) 210, 272, 2226
PdmI GAANNNNTTC 4 cut(s) 1414, 1732, 1951, 2170
PfeI GAWTC 8 cut(s) 373, 485, 604, 1004, 1541, 1556, 1931, 2042
PflFI GACNNNGTC 1 cut(s) 2591
PflMI CCANNNNNTGG 1 cut(s) 908
PkrI GCNGC 3 cut(s) 877, 1034, 1900
PleI GAGTC 5 cut(s) 1882, 2260, 2420, 2600, 2723
PpsI GAGTC 5 cut(s) 1882, 2260, 2420, 2600, 2723
Ppu21I YACGTR 1 cut(s) 1276
PpuMI RGGWCCY 1 cut(s) 2717
Psp124BI GAGCTC 1 cut(s) 1992
Psp5II RGGWCCY 1 cut(s) 2717
Psp6I CCWGG 3 cut(s) 2277, 2811, 2836
PspFI CCCAGC 3 cut(s) 2012, 2101, 2372
PspGI CCWGG 3 cut(s) 2277, 2811, 2836
PspN4I GGNNCC 7 cut(s) 151, 698, 733, 853, 1271, 2276, 2625
PspPI GGNCC 5 cut(s) 1270, 2656, 2717, 2786, 2840
PspPPI RGGWCCY 1 cut(s) 2717
PsrI GAACNNNNNNTAC 2 cut(s) 75, 107
PstNI CAGNNNCTG 3 cut(s) 875, 2572, 2720
PsuI RGATCY 2 cut(s) 737, 2623
PsyI GACNNNGTC 1 cut(s) 2591
PvuII CAGCTG 2 cut(s) 1898, 2873
RsaI GTAC 6 cut(s) 41, 1109, 1385, 1584, 1771, 2070
RsaNI GTAC 6 cut(s) 40, 1108, 1384, 1583, 1770, 2069
SacI GAGCTC 1 cut(s) 1992
SalI GTCGAC 1 cut(s) 2083
SapI GCTCTTC 3 cut(s) 210, 272, 2226
SaqAI TTAA 5 cut(s) 462, 1461, 1620, 2048, 2610
SatI GCNGC 3 cut(s) 876, 1033, 1899
Sau96I GGNCC 5 cut(s) 1270, 2656, 2717, 2786, 2840
SchI GAGTC 5 cut(s) 1883, 2261, 2421, 2601, 2723
ScrFI CCNGG 3 cut(s) 2279, 2813, 2838
SduI GDGCHC 9 cut(s) 222, 321, 478, 1310, 1768, 1992, 2398, 2453, 2797
SfaNI GCATC 6 cut(s) 14, 774, 2281, 2371, 2425, 2758
SfcI CTRYAG 5 cut(s) 1452, 1698, 1936, 1995, 2861
Sfr274I CTCGAG 1 cut(s) 2530
SinI GGWCC 4 cut(s) 1270, 2717, 2786, 2840
SlaI CTCGAG 1 cut(s) 2530
SmlI CTYRAG 3 cut(s) 980, 1460, 2530
SmoI CTYRAG 3 cut(s) 980, 1460, 2530
SpeI ACTAGT 1 cut(s) 1594
SphI GCATGC 1 cut(s) 1093
SseBI AGGCCT 1 cut(s) 412
SsiI CCGC 5 cut(s) 153, 482, 728, 2654, 2843
SspI AATATT 2 cut(s) 942, 1414
SspMI CTAG 7 cut(s) 479, 558, 1163, 1497, 1595, 2493, 2651
SstI GAGCTC 1 cut(s) 1992
StuI AGGCCT 1 cut(s) 412
StyD4I CCNGG 3 cut(s) 2277, 2811, 2836
StyI CCWWGG 3 cut(s) 1127, 1419, 2492
TaiI ACGT 5 cut(s) 648, 1278, 2132, 2191, 2690
TatI WGTACW 2 cut(s) 1582, 2068
TfiI GAWTC 8 cut(s) 373, 485, 604, 1004, 1541, 1556, 1931, 2042
Tru1I TTAA 5 cut(s) 462, 1461, 1620, 2048, 2610
Tru9I TTAA 5 cut(s) 462, 1461, 1620, 2048, 2610
TscAI CASTG 8 cut(s) 298, 326, 457, 833, 1999, 2181, 2229, 2396
TseFI GTSAC 4 cut(s) 641, 659, 1399, 2345
TseI GCWGC 3 cut(s) 875, 1032, 1898
Tsp45I GTSAC 4 cut(s) 641, 659, 1399, 2345
TspDTI ATGAA 7 cut(s) 68, 78, 539, 596, 701, 1013, 2199
TspGWI ACGGA 2 cut(s) 1401, 1549
TspRI CASTG 8 cut(s) 298, 326, 457, 833, 1999, 2181, 2229, 2396
Tth111I GACNNNGTC 1 cut(s) 2591
Van91I CCANNNNNTGG 1 cut(s) 908
Vha464I CTTAAG 1 cut(s) 1460
VneI GTGCAC 2 cut(s) 317, 2449
VpaK11BI GGWCC 4 cut(s) 1270, 2717, 2786, 2840
XapI RAATTY 9 cut(s) 169, 790, 1250, 1280, 1373, 2424, 2582, 2662, 2866
XceI RCATGY 3 cut(s) 185, 442, 1093
XhoI CTCGAG 1 cut(s) 2530
XmaJI CCTAGG 1 cut(s) 2492
XmiI GTMKAC 3 cut(s) 1213, 1701, 2084
XmnI GAANNNNTTC 4 cut(s) 1414, 1732, 1951, 2170
XspI CTAG 7 cut(s) 479, 558, 1163, 1497, 1595, 2493, 2651
ZraI GACGTC 1 cut(s) 646
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.