Rh4CG137300

metal ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0000343
Physical Location & Seq
Forward (+)
27166145 .. 27191992
25848 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG137300.1

Sequence Viewer

Length: 4026 bp
ATGGCTGCGGCACTTATAAATCTGATAGTTGGGGCAGCATCCGTCATGGTCATCGTCCTTGCTTATCTATCACACAGAATAGGTCCTTTTAGAATGGTTGTCTCCGCAAGTTTTGCCTATACTCTTGCACTGGCTCTATTATGGATTTCAGCCAAGTTTATACGTCAGGGCGCAGATGCAGTAGATAATTTTTTCTATTGGGCCGCGGTACTATTGGCAATAGCTGGCGCTGGAATGGATGCTGTTATACCTAAATTTCTTGACGATCAATACACAAGTGAACAGAGAGAAAGAGATCATGAATACACAATAGAAGAAGAAGAACGATTCCAAAGCCGCAAAGATGCTTTGTGGAGCCATCCCTGGTTTTTCGGTGCAGTGGCAGCTCTTTTCATTCCTGGAGAGGCACATTGGAAAACTGTCTTCCTGATTTCAATGATTGCAATGGGGGTTTGTTTTTTATTGTTCTGGTTTGGCAAATCCTTTTATTCAGTGTCATCTTCAGTAGAAACAGCACCAAGAGGGAAGTTTGGGGTCACACTGAAAGAAAATGGACACCGTTATGCAGAAATGTTGGCTATGAGCTTTGCCTTCATTGCATACAGTCTGGTGGAGGCAACGGGATCCACGTTCTTTTTTGAACAAACAACTTTCTTGGATGATCATGTCGGAAAATTCACAGTTCCTGTCGTATATCTAACTGTACTTCAGTCTTTTTCAAAGTTCATAATCCCACATCTTTGGGGGCTACTAATTCCAAAGCAGCGAAGAAGTGTTGCACTGGTTAAAATTGGGTGCGGTTTGGTTCTTTCTGTTTTATGCTGTGTCATTGCTTGGCGAATTGAGATTCGTAGGTCGAGAAAAATCAAGGTGGAAGTTGACCTTGAAGATTCCAAACCAATTATTTCCATGAGCATCCTTTGGTTACTTCCACAATTCTCTCTTCTTGGGCTCATGAGAGGGTTAGCTGAAGACGGATTGGTCGAGTTTTTTGCAGAACAAGTGGTTGACGATGATAAACAAGTGGTGCTTAGGTACTATGGACCTCACGTATTTTCCTTTGTCCTGGGTATTGGAACACTACTGACCGCTGGTTCTTCTCTGAATTTGGTCAGGTGTGAGTTAAACAAGGGTATGGCATCGGCAGAGTGTCAACCATATTCTGTTTCGGAGTACCATTTCGTAGATGACAAGGATGAATGTGTCTCATTTCATGTATTGCCTGTTCAATGGAGTAATGGTGAGAGACTGGATGCCGAGAAAGTAAAGATTTTTCTGCATGGAACCGCTGATAATGGGGGGAATTTATGTAAGCATGTTGTTGCATGGAAGTTTGACATTTCGAGTGAAGAGCCTGAGATATTGGTTCTCTCTGAGGAAAATAGTTGGATCAAGCTTGGGAAGCCGAGGAAGAGCTTTGAGGACACTGTTAGGTCGATCTTGATAACGGTGCACTGCCTTCACTATGTGAAGAGAAATCCTGAAAAATCCAGAGAATGTGTGTGGAATCACCTATCTGAAGTTTTCAGGTTGTATGTGGTCAGGCCTTCTCAAAGTGATCTGGTGGATCACATGCCTCTAATTGGTGATTGTGTTAACAGATATCGTGCCCTAGCGGATTCCAAGTTTTTGGCCAAACTTTTTGAAGAGAAGACAATGAAGAGGAAACTCTCTGATGAGGATACTGAAGCTAGAAAGAAGCTCAGAATTATGATCGATGATGTAAAAGATGATATGATTCATGCAGAAGATGAGGATATCGATAACCTGTTTGGTGACGTCTGTGCTTTATGTGACGATGGTGGCAAGATCATTTGTTGTGAAGGGGGGTGCCTAAGGTCTTTTCATGCAACTGAGGAGGCGGGTGCCAGATCTTATTGCAAATCTCTTGGCTTTATGCAGGATGATGTTGTTGGGATGCAAAATTTCTACTGCAAGAACTGTGAATATAAACAGCACCAGTGCTTTGTTTGTGGGAAGTTAGGTTCCTCTGATAAGTCCTCAGGTGCTGCTGAGGTGGTTTCCTGTGTATCCCCAAACTGTGGTCGATTTTACCATCCACATTGTGTTGCAATATTACTTTACCAAGATAATGGAGTTCCTGCTGAAGAACTTGAGAGAAAGATTGCTGGTGGGGAATCTTTTACTTGTCCACTTCATAAGTGCTGCATTTGTAAACAAGGAGAAAATAAGAAGGATAGTGAGTTACAATTTGCTGTGTGCATGCATTGTCCAAAATCGTACCACAGGAAATGCCTGCCAAGTGAGATTGCTTTTGAAAATCAAGGAGAAATGCTAGAAGGGAGATCAATAATAAGAGCTTGGGATGGTCTATTACCTAACCGTATACTCATATATTGCACAAAACATGATATAGATATTGAATTTGGGACTGTAAGAAGGGACCACGTAAAATTCCCTGATGTCAAACAGAATATGAGCACTCTCAAAAAGAAGAAGAAGACTATTTTAGAAGAGAAGTGGAAGCCAATATCAGAATTCCTTGTAGACGGAGACAAAGTTGGGACTAAGAGAAATATTTCCTTGGAAAAATCATGTTGGGATAAAAATGCTCCTACAGCACTTAAGACACAAAAGCCATATGTTCAAGAGAAGAAGACTAGGAAACTTATGTCAGGGCAGAAACCTGTGTCTAAGAAGAGCGATTCTTCTTTGGAAGAATCAGTTGGGGAAGGAAGTGATCCTGTACTATCCACACTAGTCAAGCAGAAGCTTTATTCAGTTAAAGAGAAGAAGGCTACTTTTGTAAAAGAGAAAACTTTTGTTGAAGATAAAGAAAGCCAACTGACATCAACGGTCTCTGTAGACAGAGAAAAAACTGTGTTGAAGAGGAGAACTCCTTCCTCTGAAGGACTATATAGTGAGAAAAGTGCTCCTACAATATCCAAGAAAAAACAGAAGCCATCTTTTGTTGAAGAGAAGAACAATGACCTTGTAAAAAAGAAAACTAATGTTGAAGAGAAGAGGAAACAGACATCAGAGTCCCTTGTTGAGAGAGAGACAGCTGTGTCCAAAAAGAAAAATCCTTCCTCGAAAGAATCCTATAGGGAAAGAAATGCTTCTACAACTCCCAAACAGAAGCAGAAGCCATCAGAGCTCACTGTAGATGGAGATGAGGCTGGGTCTAAGAAGGGAAGTCCTTTGGAAGAATCATTAAGTGCAATTGCTTCAACTGTACCCAAACTGAAGCAGAAGTCAACTTCTGCCGTGAAACTGGGTAGTAACAAGAATAGGGAAAAGACGTTTTCCGGTATAGATATTTCAAGGATGGTGAAACGAAATAATTCACTGAAAAACGAACGTCAGGCTTCCATGTCTGAGAAAATCAAGACCTCACTGGGTGAAGAGCCATTTTCTTCAATGGAGTCTGAGCAGGTGAAGCTCGGGAACCTGGATACACCTGATGCTAAAACAGTTAGTGAGATACGCAGGGAAATGCCACCACATACTGAATGTGGTGACGATGGAAATCAGCATCCTAACCCCAGCAAGTCTCGTATGCATTGTGCTCATGCTAACGGTCGAGTCCAATTCAAAATTCCTGATGATGCAGGCAGAAGGTGCACCACGAGTAATGATGAGCCTCGTTCAAGTGTTACCAATCCTAGGTCTGGCAATGCGAAAGGGTGCAAGAGAGAGAACTCGGGAAAACCATATTCAAATGGGACGAAACAGAAACAGAAACTGAGAACAGAATCTGACAGAGTCCCACCGGCTTGCATTAAAGGGAAGCAAGATCCTTTTTTTTCAAGAGGCAATGATCTAGCGGGCCAAAATTCAGTATATGACCATATTAGGACGTTCTCATCTACTTTTTACAGCCATACAGGTCCTGTTGCTGACTCGTTATCTAGTATCAACAGATTAGCTATGCCATCAGATGCAACTAAATTGGTTGGTCCAAGAGCACCCCAACCTGATTACCTGGATAGGTGTATGGGTTTCGCTCCTGGTCCGCACCTGAACTATTCACTACAGAATTCAGCTGGTTGGATCGATGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006325 GO:0006355 GO:0006950 GO:0006952 GO:0006996 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0009605 GO:0009607 GO:0009620 GO:0009791 GO:0009889 GO:0009893 GO:0009909 GO:0009911 GO:0009987 GO:0010228 GO:0010468 GO:0010556 GO:0010604 GO:0010638 GO:0016043 GO:0019219 GO:0019222 GO:0022414 GO:0023052 GO:0030154 GO:0031056 GO:0031058 GO:0031060 GO:0031062 GO:0031323 GO:0031325 GO:0031326 GO:0031399 GO:0031401 GO:0031440 GO:0031935 GO:0031937 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033043 GO:0033044 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0043954 GO:0044030 GO:0044424 GO:0044464 GO:0048366 GO:0048367 GO:0048468 GO:0048518 GO:0048522 GO:0048580 GO:0048582 GO:0048608 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0048869 GO:0050684 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051052 GO:0051094 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051276 GO:0051570 GO:0051574 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060968 GO:0061085 GO:0061087 GO:0061458 GO:0065007 GO:0070827 GO:0070828 GO:0070829 GO:0071840 GO:0080090 GO:0090436 GO:0097159 GO:0098542 GO:0099402 GO:0140110 GO:1900109 GO:1900111 GO:1900363 GO:1901363 GO:1902275 GO:1903311 GO:1903506 GO:1905269 GO:2000024 GO:2000026 GO:2000112 GO:2000241 GO:2000243 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNMT1-RFD

Protein Analysis

1341

Amino Acids

150.46

Weight (kDa)

8.6

Isoelectric Point (pI)

45.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 181 - 343 1.5e-10 POT family
DNMT1-RFD PF12047 389 - 510 4.2e-10 Cytosine specific DNA methyltransferase replication foci domain
PHD_NSD PF22908 651 - 720 5e-19 Histone-lysine N-methyltransferase NSD-like, PHD zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000343)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48090 AT5G48090 AT5G48090 AT5G55390 AT5G55390 AT5G55390
fragaria_vesca FvH4_3g23690 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160
malus_domestica MD03G1191000.v1.1 MD11G1207800.v1.1 MD11G1208000.v1.1
prunus_persica Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227600_v2.0.a1 Prupe.4G227700_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1
pyrus_communis pycom01g03400 pycom03g14420 pycom11g18100 pycom11g18140 pycom11g18150 pycom11g18160
rosa_chinensis RchiOBHm_Chr2g0145981 RchiOBHm_Chr4g0405241 RchiOBHm_Chr4g0405271 RchiOBHm_Chr5g0016201 RchiOBHm_Chr5g0041831 RchiOBHm_Chr5g0041871 RchiOBHm_Chr5g0041881 RchiOBHm_Chr5g0059241
rosa_laevigata RLG00000002117 RLG00000008861 RLG00000008862 RLG00000011000 RLG00000033216 RLG00000034097 RLG00000034101
rosa_multiflora Rmu_sc0000161.1_g000001 Rmu_sc0000861.1_g000040 Rmu_sc0007522.1_g000003 Rmu_sc0009443.1_g000015 Rmu_sc0022129.1_g000001 Rmu_sc0042315.1_g000001
rosa_roxburghii Rroxscaffold_1G00038660 Rroxscaffold_1G00038690 Rroxscaffold_5G00349560 Rroxscaffold_5G00349610 Rroxscaffold_6G00388370 Rroxscaffold_7G00188740
rosa_rugosa Rorug03G0199700 Rorug04G0055400 Rorug04G0055500 Rorug04G0055900 Rorug04G0056000 Rorug04G0056100 Rorug04G0056100 Rorug04G0056200 Rorug05G0195600 Rorug05G0195700 Rorug05G0195800 Rorug05G0195900 Rorug05G0196800 Rorug05G0385000
rosa_samantha Rh4AG129900 Rh4AG130100 Rh4BG124200 Rh4BG124400 Rh4BG124500 Rh4CG137200 Rh4CG137300 Rh4DG123500 Rh4DG123700 Rh5AG280800 Rh5AG281400 Rh5BG286700 Rh5BG287000 Rh5BG397800 Rh5CG318100 Rh5CG318600 Rh5CG421600 Rh5DG132300 Rh5DG132400 Rh5DG295400 Rh5DG295700 Rh7BG475300
rosa_wichuraiana Rw0G001330 Rw0G005730 Rw4G008670 Rw4G010510 Rw4G010540 Rw5G026450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 17
AarI CACCTGC 1 cut(s) 3385
AasI GACNNNNNNGTC 3 cut(s) 980, 3001, 3028
AatII GACGTC 1 cut(s) 1782
Acc36I ACCTGC 1 cut(s) 3385
AccB1I GGYRCC 2 cut(s) 1830, 1865
AccB7I CCANNNNNTGG 1 cut(s) 2042
AccI GTMKAC 3 cut(s) 2347, 2508, 2826
AccII CGCG 1 cut(s) 206
AclWI GGATC 7 cut(s) 618, 631, 1397, 1575, 2696, 3752, 4022
AcoI YGGCCR 1 cut(s) 1632
AcuI CTGAAG 8 cut(s) 486, 692, 990, 1539, 1707, 2127, 2889, 3227
AcyI GRCGYC 1 cut(s) 1779
AdeI CACNNNGTG 3 cut(s) 1468, 2066, 3362
AfaI GTAC 7 cut(s) 210, 705, 1037, 1175, 2243, 2709, 3198
AfiI CCNNNNNNNGG 4 cut(s) 1583, 2042, 3627, 3632
AflII CTTAAG 1 cut(s) 2585
AhlI ACTAGT 1 cut(s) 2719
AjnI CCWGG 6 cut(s) 362, 397, 1065, 3411, 3945, 3970
AjuI GAANNNNNNNTTGG 4 cut(s) 2670, 2702, 3543, 3575
AloI GAACNNNNNNTCC 2 cut(s) 1969, 2001
Alw21I GWGCWC 7 cut(s) 1455, 2444, 2896, 3120, 3532, 3587, 3931
Alw26I GTCTC 7 cut(s) 106, 1210, 1240, 2508, 2824, 3014, 3519
Alw44I GTGCAC 2 cut(s) 1451, 3583
AlwI GGATC 7 cut(s) 618, 631, 1397, 1575, 2696, 3752, 4022
AlwNI CAGNNNCTG 5 cut(s) 686, 2009, 3706, 3719, 3854
Ama87I CYCGRG 2 cut(s) 3404, 3664
AoxI GGCC 4 cut(s) 201, 1544, 1632, 3790
ApaLI GTGCAC 2 cut(s) 1451, 3583
ApeKI GCWGC 6 cut(s) 5, 35, 383, 763, 2009, 2166
Asp700I GAANNNNTTC 4 cut(s) 2539, 2860, 3079, 3304
AspA2I CCTAGG 1 cut(s) 3626
AspLEI GCGC 2 cut(s) 173, 230
AspS9I GGNCC 8 cut(s) 83, 201, 1043, 2404, 3790, 3851, 3920, 3974
AsuHPI GGTGA 8 cut(s) 1253, 1502, 1598, 1787, 3304, 3374, 3409, 3491
AvaI CYCGRG 2 cut(s) 3404, 3664
AvaII GGWCC 6 cut(s) 83, 1043, 2404, 3851, 3920, 3974
AvrII CCTAGG 1 cut(s) 3626
AxyI CCTNAGG 2 cut(s) 1835, 2002
BaeGI GKGCMC 3 cut(s) 1455, 1612, 3587
BalI TGGCCA 1 cut(s) 1634
BamHI GGATCC 1 cut(s) 623
BanI GGYRCC 2 cut(s) 1830, 1865
BanII GRGCYC 2 cut(s) 954, 3120
BauI CACGAG 1 cut(s) 3589
BbsI GAAGAC 5 cut(s) 415, 978, 1658, 2468, 2624
Bbv12I GWGCWC 7 cut(s) 1455, 2444, 2896, 3120, 3532, 3587, 3931
BbvCI CCTCAGC 1 cut(s) 2013
BbvI GCAGC 5 cut(s) 47, 395, 775, 1996, 2153
BceAI ACGGC 1 cut(s) 3212
BcgI CGANNNNNNTGC 2 cut(s) 974, 1008
BciT130I CCWGG 6 cut(s) 364, 399, 1067, 3413, 3947, 3972
BciVI GTATCC 3 cut(s) 1675, 2041, 3409
BclI TGATCA 1 cut(s) 661
BcoDI GTCTC 7 cut(s) 106, 1210, 1240, 2508, 2824, 3014, 3519
BcuI ACTAGT 1 cut(s) 2719
BfaI CTAG 8 cut(s) 1613, 1692, 2297, 2622, 2720, 3627, 3785, 3873
BfmI CTRYAG 5 cut(s) 2577, 2823, 3064, 3123, 3995
BfoI RGCGCY 1 cut(s) 231
BfrI CTTAAG 1 cut(s) 2585
BfuAI ACCTGC 1 cut(s) 3385
BfuI GTATCC 3 cut(s) 1675, 2041, 3409
BglII AGATCT 1 cut(s) 1871
BisI GCNGC 9 cut(s) 6, 9, 36, 204, 337, 384, 764, 2010, 2167
BlnI CCTAGG 1 cut(s) 3626
BlsI GCNGC 9 cut(s) 7, 10, 37, 205, 338, 385, 765, 2011, 2168
Bme1390I CCNGG 6 cut(s) 364, 399, 1067, 3413, 3947, 3972
Bme18I GGWCC 6 cut(s) 83, 1043, 2404, 3851, 3920, 3974
BmeT110I CYCGRG 2 cut(s) 3404, 3664
BmgT120I GGNCC 8 cut(s) 83, 201, 1043, 2404, 3790, 3851, 3920, 3974
BmiI GGNNCC 8 cut(s) 356, 625, 1285, 1832, 1867, 1987, 2405, 3410
BmrFI CCNGG 6 cut(s) 364, 399, 1067, 3413, 3947, 3972
BmrI ACTGGG 2 cut(s) 3245, 3368
BmuI ACTGGG 2 cut(s) 3245, 3368
BpiI GAAGAC 5 cut(s) 415, 978, 1658, 2468, 2624
BplI GAGNNNNNCTC 2 cut(s) 2842, 2874
BpmI CTGGAG 1 cut(s) 420
Bpu10I CCTNAGC 2 cut(s) 1031, 2013
BpuEI CTTGAG 1 cut(s) 2135
Bsa29I ATCGAT 3 cut(s) 1716, 1761, 4017
BsaAI YACGTR 2 cut(s) 1051, 2410
BsaBI GATNNNNATC 1 cut(s) 3489
BsaHI GRCGYC 1 cut(s) 1779
BsaI GGTCTC 1 cut(s) 2824
BsaJI CCNNGG 6 cut(s) 204, 362, 1066, 1406, 2544, 3626
BsaWI WCCGGW 1 cut(s) 3269
BsaXI ACNNNNNCTCC 4 cut(s) 1225, 1255, 2505, 2535
Bsc4I CCNNNNNNNGG 4 cut(s) 1583, 2042, 3627, 3632
Bse118I RCCGGY 1 cut(s) 3733
Bse1I ACTGG 6 cut(s) 135, 786, 1254, 1960, 3240, 3363
Bse21I CCTNAGG 2 cut(s) 1835, 2002
Bse3DI GCAATG 5 cut(s) 450, 594, 828, 3643, 3784
Bse8I GATNNNNATC 1 cut(s) 3489
BseBI CCWGG 6 cut(s) 364, 399, 1067, 3413, 3947, 3972
BseCI ATCGAT 3 cut(s) 1716, 1761, 4017
BseDI CCNNGG 6 cut(s) 204, 362, 1066, 1406, 2544, 3626
BseJI GATNNNNATC 1 cut(s) 3489
BseLI CCNNNNNNNGG 4 cut(s) 1583, 2042, 3627, 3632
BseMI GCAATG 5 cut(s) 450, 594, 828, 3643, 3784
BseMII CTCAG 9 cut(s) 1347, 1365, 1717, 1845, 2004, 2016, 3330, 3381, 3698
BseNI ACTGG 6 cut(s) 135, 786, 1254, 1960, 3240, 3363
BseRI GAGGAG 2 cut(s) 1871, 2866
BseSI GKGCMC 3 cut(s) 1455, 1612, 3587
BseXI GCAGC 5 cut(s) 47, 395, 775, 1996, 2153
BseYI CCCAGC 2 cut(s) 3140, 3506
BsgI GTGCAG 1 cut(s) 396
Bsh1236I CGCG 1 cut(s) 206
Bsh1285I CGRYCG 1 cut(s) 3544
BshFI GGCC 4 cut(s) 203, 1546, 1634, 3792
BshNI GGYRCC 2 cut(s) 1830, 1865
BshVI ATCGAT 3 cut(s) 1716, 1761, 4017
BsiEI CGRYCG 1 cut(s) 3544
BsiHKAI GWGCWC 7 cut(s) 1455, 2444, 2896, 3120, 3532, 3587, 3931
BsiHKCI CYCGRG 2 cut(s) 3404, 3664
BsiSI CCGG 2 cut(s) 3270, 3734
BslFI GGGAC 6 cut(s) 2404, 2417, 2539, 2989, 3700, 3713
BslI CCNNNNNNNGG 4 cut(s) 1583, 2042, 3627, 3632
BsmAI GTCTC 7 cut(s) 106, 1210, 1240, 2508, 2824, 3014, 3519
BsmFI GGGAC 6 cut(s) 2404, 2417, 2539, 2989, 3700, 3713
BsnI GGCC 4 cut(s) 203, 1546, 1634, 3792
Bso31I GGTCTC 1 cut(s) 2824
BsoBI CYCGRG 2 cut(s) 3404, 3664
Bsp1286I GDGCHC 9 cut(s) 954, 1455, 1612, 2444, 2896, 3120, 3532, 3587, 3931
BspANI GGCC 4 cut(s) 203, 1546, 1634, 3792
BspCNI CTCAG 9 cut(s) 1348, 1366, 1716, 1846, 2005, 2015, 3331, 3382, 3699
BspDI ATCGAT 3 cut(s) 1716, 1761, 4017
BspFNI CGCG 1 cut(s) 206
BspHI TCATGA 2 cut(s) 298, 954
BspLI GGNNCC 8 cut(s) 356, 625, 1285, 1832, 1867, 1987, 2405, 3410
BspMI ACCTGC 1 cut(s) 3385
BspPI GGATC 7 cut(s) 618, 631, 1397, 1575, 2696, 3752, 4022
BspQI GCTCTTC 4 cut(s) 1344, 1406, 2654, 3360
BspT107I GGYRCC 2 cut(s) 1830, 1865
BspTI CTTAAG 1 cut(s) 2585
BspTNI GGTCTC 1 cut(s) 2824
BsrDI GCAATG 5 cut(s) 450, 594, 828, 3643, 3784
BsrFI RCCGGY 1 cut(s) 3733
BsrI ACTGG 6 cut(s) 135, 786, 1254, 1960, 3240, 3363
BssAI RCCGGY 1 cut(s) 3733
BssECI CCNNGG 6 cut(s) 204, 362, 1066, 1406, 2544, 3626
BssNAI GTATAC 1 cut(s) 2348
BssNI GRCGYC 1 cut(s) 1779
BssSI CACGAG 1 cut(s) 3589
BssT1I CCWWGG 2 cut(s) 2544, 3626
Bst1107I GTATAC 1 cut(s) 2348
Bst2BI CACGAG 1 cut(s) 3589
Bst2UI CCWGG 6 cut(s) 364, 399, 1067, 3413, 3947, 3972
BstACI GRCGYC 1 cut(s) 1779
BstAFI CTTAAG 1 cut(s) 2585
BstAPI GCANNNNNTGC 2 cut(s) 113, 3582
BstBAI YACGTR 2 cut(s) 1051, 2410
BstC8I GCNNGC 6 cut(s) 226, 2225, 2258, 3574, 3739, 3790
BstDSI CCRYGG 1 cut(s) 204
BstFNI CGCG 1 cut(s) 206
BstH2I RGCGCY 1 cut(s) 231
BstHHI GCGC 2 cut(s) 173, 230
BstMAI GTCTC 7 cut(s) 106, 1210, 1240, 2508, 2824, 3014, 3519
BstMCI CGRYCG 1 cut(s) 3544
BstMWI GCNNNNNNNGC 8 cut(s) 113, 383, 596, 1402, 2579, 3115, 3400, 3582
BstNI CCWGG 6 cut(s) 364, 399, 1067, 3413, 3947, 3972
BstNSI RCATGY 3 cut(s) 1319, 1576, 2227
BstSCI CCNGG 6 cut(s) 362, 397, 1065, 3411, 3945, 3970
BstSFI CTRYAG 5 cut(s) 2577, 2823, 3064, 3123, 3995
BstSLI GKGCMC 3 cut(s) 1455, 1612, 3587
BstUI CGCG 1 cut(s) 206
BstV1I GCAGC 5 cut(s) 47, 395, 775, 1996, 2153
BstV2I GAAGAC 5 cut(s) 415, 978, 1658, 2468, 2624
BstX2I RGATCY 3 cut(s) 623, 1871, 3757
BstXI CCANNNNNNTGG 3 cut(s) 741, 1630, 2093
BstYI RGATCY 3 cut(s) 623, 1871, 3757
BstZ17I GTATAC 1 cut(s) 2348
Bsu15I ATCGAT 3 cut(s) 1716, 1761, 4017
Bsu36I CCTNAGG 2 cut(s) 1835, 2002
BsuI GTATCC 3 cut(s) 1675, 2041, 3409
BsuRI GGCC 4 cut(s) 203, 1546, 1634, 3792
BsuTUI ATCGAT 3 cut(s) 1716, 1761, 4017
BtgI CCRYGG 1 cut(s) 204
BtsI GCAGTG 2 cut(s) 384, 1453
BveI ACCTGC 1 cut(s) 3385
Cac8I GCNNGC 6 cut(s) 226, 2225, 2258, 3574, 3739, 3790
CaiI CAGNNNCTG 5 cut(s) 686, 2009, 3706, 3719, 3854
CciI TCATGA 2 cut(s) 298, 954
CfoI GCGC 2 cut(s) 173, 230
Cfr10I RCCGGY 1 cut(s) 3733
Cfr13I GGNCC 8 cut(s) 83, 201, 1043, 2404, 3790, 3851, 3920, 3974
Cfr42I CCGCGG 1 cut(s) 207
ClaI ATCGAT 3 cut(s) 1716, 1761, 4017
Csp6I GTAC 7 cut(s) 209, 704, 1036, 1174, 2242, 2708, 3197
CviQI GTAC 7 cut(s) 209, 704, 1036, 1174, 2242, 2708, 3197
DraIII CACNNNGTG 3 cut(s) 1468, 2066, 3362
DrdI GACNNNNNNGTC 3 cut(s) 980, 3001, 3028
DseDI GACNNNNNNGTC 3 cut(s) 980, 3001, 3028
EaeI YGGCCR 1 cut(s) 1632
Ecl136II GAGCTC 1 cut(s) 3118
Eco130I CCWWGG 2 cut(s) 2544, 3626
Eco147I AGGCCT 1 cut(s) 1546
Eco24I GRGCYC 2 cut(s) 954, 3120
Eco31I GGTCTC 1 cut(s) 2824
Eco32I GATATC 2 cut(s) 1604, 1759
Eco47I GGWCC 6 cut(s) 83, 1043, 2404, 3851, 3920, 3974
Eco53kI GAGCTC 1 cut(s) 3118
Eco57I CTGAAG 8 cut(s) 486, 692, 990, 1539, 1707, 2127, 2889, 3227
Eco81I CCTNAGG 2 cut(s) 1835, 2002
Eco88I CYCGRG 2 cut(s) 3404, 3664
EcoICRI GAGCTC 1 cut(s) 3118
EcoO109I RGGNCCY 2 cut(s) 83, 3851
EcoRI GAATTC 2 cut(s) 2498, 4000
EcoRII CCWGG 6 cut(s) 362, 397, 1065, 3411, 3945, 3970
EcoRV GATATC 2 cut(s) 1604, 1759
EcoT14I CCWWGG 2 cut(s) 2544, 3626
EcoT22I ATGCAT 2 cut(s) 2229, 3525
EcoT38I GRGCYC 2 cut(s) 954, 3120
ErhI CCWWGG 2 cut(s) 2544, 3626
FalI AAGNNNNNCTT 6 cut(s) 867, 899, 1014, 1046, 3064, 3096
FaqI GGGAC 6 cut(s) 2404, 2417, 2539, 2989, 3700, 3713
FauI CCCGC 2 cut(s) 1855, 3781
FauNDI CATATG 1 cut(s) 2602
FbaI TGATCA 1 cut(s) 661
FblI GTMKAC 3 cut(s) 2347, 2508, 2826
Fnu4HI GCNGC 9 cut(s) 6, 9, 36, 204, 337, 384, 764, 2010, 2167
FriOI GRGCYC 2 cut(s) 954, 3120
Fsp4HI GCNGC 9 cut(s) 6, 9, 36, 204, 337, 384, 764, 2010, 2167
FspBI CTAG 8 cut(s) 1613, 1692, 2297, 2622, 2720, 3627, 3785, 3873
GlaI GCGC 2 cut(s) 172, 229
GluI GCNGC 9 cut(s) 6, 9, 36, 204, 337, 384, 764, 2010, 2167
GsaI CCCAGC 2 cut(s) 3144, 3510
GsuI CTGGAG 1 cut(s) 420
HaeII RGCGCY 1 cut(s) 231
HaeIII GGCC 4 cut(s) 203, 1546, 1634, 3792
HapII CCGG 2 cut(s) 3270, 3734
HhaI GCGC 2 cut(s) 173, 230
Hin1I GRCGYC 1 cut(s) 1779
Hin6I GCGC 2 cut(s) 171, 228
HinP1I GCGC 2 cut(s) 171, 228
HincII GTYRAC 5 cut(s) 880, 1009, 1154, 1597, 3219
HindII GTYRAC 5 cut(s) 880, 1009, 1154, 1597, 3219
HindIII AAGCTT 2 cut(s) 1394, 2732
HpaI GTTAAC 1 cut(s) 1597
HpaII CCGG 2 cut(s) 3270, 3734
HphI GGTGA 8 cut(s) 1253, 1502, 1598, 1787, 3304, 3374, 3409, 3491
HpyCH4IV ACGT 8 cut(s) 163, 629, 1050, 1779, 2409, 3263, 3322, 3821
HpyF10VI GCNNNNNNNGC 8 cut(s) 113, 383, 596, 1402, 2579, 3115, 3400, 3582
HpySE526I ACGT 8 cut(s) 163, 629, 1050, 1779, 2409, 3263, 3322, 3821
Hsp92I GRCGYC 1 cut(s) 1779
HspAI GCGC 2 cut(s) 171, 228
Ksp22I TGATCA 1 cut(s) 661
KspAI GTTAAC 1 cut(s) 1597
KspI CCGCGG 1 cut(s) 207
LguI GCTCTTC 4 cut(s) 1344, 1406, 2654, 3360
LmnI GCTCC 4 cut(s) 354, 2578, 2899, 3973
Lsp1109I GCAGC 5 cut(s) 47, 395, 775, 1996, 2153
MaeI CTAG 8 cut(s) 1613, 1692, 2297, 2622, 2720, 3627, 3785, 3873
MaeII ACGT 8 cut(s) 163, 629, 1050, 1779, 2409, 3263, 3322, 3821
MaeIII GTNAC 8 cut(s) 535, 924, 1775, 1793, 2205, 3242, 3479, 3616
MfeI CAATTG 1 cut(s) 3183
MflI RGATCY 3 cut(s) 623, 1871, 3757
MhlI GDGCHC 9 cut(s) 954, 1455, 1612, 2444, 2896, 3120, 3532, 3587, 3931
MlsI TGGCCA 1 cut(s) 1634
MluNI TGGCCA 1 cut(s) 1634
MlyI GAGTC 5 cut(s) 3011, 3395, 3555, 3735, 3857
MmeI TCCRAC 3 cut(s) 649, 1367, 3992
Mox20I TGGCCA 1 cut(s) 1634
Mph1103I ATGCAT 2 cut(s) 2229, 3525
MroXI GAANNNNTTC 4 cut(s) 2539, 2860, 3079, 3304
MscI TGGCCA 1 cut(s) 1634
MseI TTAA 7 cut(s) 786, 1124, 1596, 2586, 2745, 3176, 3744
MslI CAYNNNNRTG 1 cut(s) 561
Msp20I TGGCCA 1 cut(s) 1634
MspA1I CMGCKG 5 cut(s) 206, 1091, 1289, 3026, 4007
MspCI CTTAAG 1 cut(s) 2585
MspI CCGG 2 cut(s) 3270, 3734
MspR9I CCNGG 6 cut(s) 364, 399, 1067, 3413, 3947, 3972
MunI CAATTG 1 cut(s) 3183
MvaI CCWGG 6 cut(s) 364, 399, 1067, 3413, 3947, 3972
MvnI CGCG 1 cut(s) 206
MwoI GCNNNNNNNGC 8 cut(s) 113, 383, 596, 1402, 2579, 3115, 3400, 3582
NdeI CATATG 1 cut(s) 2602
NlaIV GGNNCC 8 cut(s) 356, 625, 1285, 1832, 1867, 1987, 2405, 3410
NmeAIII GCCGAG 2 cut(s) 1282, 1431
NmuCI GTSAC 4 cut(s) 535, 1775, 1793, 3479
NsiI ATGCAT 2 cut(s) 2229, 3525
NspI RCATGY 3 cut(s) 1319, 1576, 2227
PaeI GCATGC 1 cut(s) 2227
PagI TCATGA 2 cut(s) 298, 954
PaqCI CACCTGC 1 cut(s) 3385
PceI AGGCCT 1 cut(s) 1546
PciSI GCTCTTC 4 cut(s) 1344, 1406, 2654, 3360
PcsI WCGNNNNNNNCGW 2 cut(s) 626, 981
PdmI GAANNNNTTC 4 cut(s) 2539, 2860, 3079, 3304
PflFI GACNNNGTC 1 cut(s) 3725
PflMI CCANNNNNTGG 1 cut(s) 2042
PfoI TCCNGGA 1 cut(s) 397
PkrI GCNGC 9 cut(s) 7, 10, 37, 205, 338, 385, 765, 2011, 2168
PleI GAGTC 5 cut(s) 3010, 3394, 3554, 3734, 3857
PpsI GAGTC 5 cut(s) 3010, 3394, 3554, 3734, 3857
Ppu21I YACGTR 2 cut(s) 1051, 2410
PpuMI RGGWCCY 2 cut(s) 83, 3851
PsiI TTATAA 1 cut(s) 17
Psp124BI GAGCTC 1 cut(s) 3120
Psp5II RGGWCCY 2 cut(s) 83, 3851
Psp6I CCWGG 6 cut(s) 362, 397, 1065, 3411, 3945, 3970
PspFI CCCAGC 2 cut(s) 3140, 3506
PspGI CCWGG 6 cut(s) 362, 397, 1065, 3411, 3945, 3970
PspN4I GGNNCC 8 cut(s) 356, 625, 1285, 1832, 1867, 1987, 2405, 3410
PspPI GGNCC 8 cut(s) 83, 201, 1043, 2404, 3790, 3851, 3920, 3974
PspPPI RGGWCCY 2 cut(s) 83, 3851
PsrI GAACNNNNNNTAC 2 cut(s) 1209, 1241
PstNI CAGNNNCTG 5 cut(s) 686, 2009, 3706, 3719, 3854
PsuI RGATCY 3 cut(s) 623, 1871, 3757
PsyI GACNNNGTC 1 cut(s) 3725
PvuII CAGCTG 2 cut(s) 3026, 4007
RsaI GTAC 7 cut(s) 210, 705, 1037, 1175, 2243, 2709, 3198
RsaNI GTAC 7 cut(s) 209, 704, 1036, 1174, 2242, 2708, 3197
RseI CAYNNNNRTG 1 cut(s) 561
SacI GAGCTC 1 cut(s) 3120
SacII CCGCGG 1 cut(s) 207
SapI GCTCTTC 4 cut(s) 1344, 1406, 2654, 3360
SaqAI TTAA 7 cut(s) 786, 1124, 1596, 2586, 2745, 3176, 3744
SatI GCNGC 9 cut(s) 6, 9, 36, 204, 337, 384, 764, 2010, 2167
Sau96I GGNCC 8 cut(s) 83, 201, 1043, 2404, 3790, 3851, 3920, 3974
SchI GAGTC 5 cut(s) 3011, 3395, 3555, 3735, 3857
ScrFI CCNGG 6 cut(s) 364, 399, 1067, 3413, 3947, 3972
SduI GDGCHC 9 cut(s) 954, 1455, 1612, 2444, 2896, 3120, 3532, 3587, 3931
SfcI CTRYAG 5 cut(s) 2577, 2823, 3064, 3123, 3995
Sfr303I CCGCGG 1 cut(s) 207
SgrBI CCGCGG 1 cut(s) 207
SinI GGWCC 6 cut(s) 83, 1043, 2404, 3851, 3920, 3974
SmiMI CAYNNNNRTG 1 cut(s) 561
SmlI CTYRAG 2 cut(s) 2114, 2585
SmoI CTYRAG 2 cut(s) 2114, 2585
SpeI ACTAGT 1 cut(s) 2719
SphI GCATGC 1 cut(s) 2227
SseBI AGGCCT 1 cut(s) 1546
SspI AATATT 2 cut(s) 2076, 2539
SspMI CTAG 8 cut(s) 1613, 1692, 2297, 2622, 2720, 3627, 3785, 3873
SstI GAGCTC 1 cut(s) 3120
StuI AGGCCT 1 cut(s) 1546
StyD4I CCNGG 6 cut(s) 362, 397, 1065, 3411, 3945, 3970
StyI CCWWGG 2 cut(s) 2544, 3626
TaiI ACGT 8 cut(s) 166, 632, 1053, 1782, 2412, 3266, 3325, 3824
TatI WGTACW 2 cut(s) 703, 2707
TauI GCSGC 3 cut(s) 11, 206, 339
Tru1I TTAA 7 cut(s) 786, 1124, 1596, 2586, 2745, 3176, 3744
Tru9I TTAA 7 cut(s) 786, 1124, 1596, 2586, 2745, 3176, 3744
TseFI GTSAC 4 cut(s) 535, 1775, 1793, 3479
TseI GCWGC 6 cut(s) 5, 35, 383, 763, 2009, 2166
Tsp45I GTSAC 4 cut(s) 535, 1775, 1793, 3479
TspGWI ACGGA 3 cut(s) 31, 990, 2526
Tth111I GACNNNGTC 1 cut(s) 3725
Van91I CCANNNNNTGG 1 cut(s) 2042
Vha464I CTTAAG 1 cut(s) 2585
VneI GTGCAC 2 cut(s) 1451, 3583
VpaK11BI GGWCC 6 cut(s) 83, 1043, 2404, 3851, 3920, 3974
XceI RCATGY 3 cut(s) 1319, 1576, 2227
XmaJI CCTAGG 1 cut(s) 3626
XmiI GTMKAC 3 cut(s) 2347, 2508, 2826
XmnI GAANNNNTTC 4 cut(s) 2539, 2860, 3079, 3304
XspI CTAG 8 cut(s) 1613, 1692, 2297, 2622, 2720, 3627, 3785, 3873
ZraI GACGTC 1 cut(s) 1780
Zsp2I ATGCAT 2 cut(s) 2229, 3525
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.