Rorug05G0195800

metal ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000343
Physical Location & Seq
Forward (+)
19283155 .. 19285761
2607 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0195800.1

Sequence Viewer

Length: 2607 bp
ATGGCGGAACTTGCAATTCCTTTATCATTGTTGTGTGCCGAGAAGGCGATGAGCTTGTTCGAAATAAACAAGTCCAACGACCGAAGTACTAATATCCATGATGATGTTGAAAGAGCCTGCAAATGTCTGAAGAAGATGCAAGCCTATCTAGGGGACTCGGAAGAGATTAATGAAGGCAGCCGAATGCTTCAAAATCGTGTCGAAGAGGTCCAAAATCTGGCTTATGAAATTGAAGATGCTCTTGACAAGTTCATGGCTCAGATTCCACATCACTTCCACAGCAACATGTTCTCTCAGAAGCTGCACGACGTTGCCCATTATCGAACAGAAAAGAACGCAAGAGTTGAGTTCTCCACCAAGATTAAAGATATTGAAACGAAAATTAGTTCCTTCTCAGGACTGAATTCAATTCTTGAGCCACCAAGTTTCAGCACAAGGCAGAGAGATAGTTTAATCCACCAAGTTCTTGAAGAAGACGAAATCGTAGGGTTTGAGGAGCCTAGAGAAAGACTTTCCAAACAGTTGTTGGAAGTAGATTTGAGGCTCTTGATGATTTTGATTGTTGGTCCTGGCGGCTCCGGCAAAACCACTGTCTTGAAGAATGTTTATGACAGCAAGAGCATTCAAGAATTTTTTGATTGCCTTGCTTGGATTGATGTGTCCCGCCCTTCTAAAGCAGAAGAAGATCAAAAAGCAGAACTCAGTCATCTGAAGGGAAAGAGATTTGTGGTTGTTTTGGATAATGTTTGGACAACAAATGATCTGGAACGCATCACAAATGCTTTGCCAAAAGGATTGCTTGGGAGTAAAGTAATCATAACTACTCGTAACTCTGATATAGCTTCTTCTCGTGTAGAGTCCTCCGAGTATATCCATGATTTGAGTTGCGGATTATTATGGAAAGATGCCTGGTGTCTATTTTGCAACAAGGCTTTCAAACATACTGCAGGATATTGTCCACCTGAGCTCGAAGAGTGGGCTAAAAATTTTCTGAAGAAGTGTGAAGGTCTGCCCCTTGCAATTTCAGCTATTGCTACTTTGCTTGCAAAGAAGCCACAAACTCCAATTGAGTGGAAAAAGTTACATCAAAGCCTTGGGTCTGAAATTAGACCTCATTTTAGTCTTTCGATTATTAGCCGTGTCTTGCAGCCAAGTTACATGCATCTTCCGAGCCATCTTAAAATATGTTTCTCGTACTTTGGCATGTTTCCAGAAGACTATTCCATCAGCCGTGACAGGCTTATTCGATTGTGGTTAGCTGAAGGATTTGTAAAACCGGGAAAAGGACAAACAATGGAAGAAGCTGGGGAAAGTTATCTCAATGAGCTCATCGGAAGGAATTTGGTTCATGCCACCACAAGAGAGATGGATGGTCGTGTAAGGAGCTGTCGTGTTTTCAACCTTGTGCGAGAGTTCATCATTTCAAAAGAGGGGAACTTCATCACTGTATTGGAACCAAACTGCAGTATTGCTGCTCATTCTGGTGAGAAAATTCGGCGCCTTTCGGTTCAAAATGTCAATATTAATTTGTTAAAGGATAAGGAGTTAAGTCAGATTCGTACCTTACTAGTGTTTGGGCAGGCAAGTTTCCCTTCCGAGCTTGAAGTGGTACTTAAGACCTGTACATACTTGAGGGTGTTAGATATGCAAGGTGTACCTTTAAAGAACTTCCCTGACAATGTTATTGGTCTCCCCCTCTTAAGGTACTTAAATCTGAGCCAAACTGAAATCAAATCAGTTCCAAAGTCCATCGGCAATCTTGCATTTTTGCAAACCTTAGATCTTAAGCACACACAGGTGACCAGCTTACCAAAAAAGATTTATGAACTCCAGGATATGCGTCATCTTTTAGTTTATAGCTGTGATGTTACTTCTGGTGTAGCACAAGGAGTTGAGGTTCCTGCAGGCAACATTGGAGCTTTGTCTTCCATACAGAAGTTGTTTCTAATCAAGGTGAAAAAGCAAAGAAAAGTAGTCCTTCAAGCCTTGGGACAGTTAGTTGCTCTCCGGAAGTTGGGTCTGGTCGATCTCGAAAATGAGCATGGAAGAGAACTGTGCTGTTCCATCCAGAAAATGAGAGAGCTTTCGACACTTGACATACGATCAACAAGTGAGGCCGAGTATCTTGATTTGGATCATATGAACTCCCCTGATGGGCCTCCTCGCTATCTACAACGTCTCTCTTTGACAGGGCGCCTGGAGAGTCTGCCGCAGTGGATCTCCCAGCTTCATAGTCTAGCTAGGATTGCTTTAAAGAGATCAAAATTGGATGCTGATTCCAGCTCACTTGAGGCCCTTCAAGATTTGCCTAATCTTGTGGAACTCGAGTTGGTTGACTTTTATACTGGGGATTTGTTGAAGTTCAGAGCTAAAAAGTTCAAGAAACTACAGGTATTACGCATTGAGCAATTTGATGAGCTAGATTTGATGATAATCGAGGATGGAGCAATGCCTGAGCTTAAGAGGCTAACATTGTCTAAATGTCGAAATCTAAAGTTACTTCTATTTGGCATTGGTAGTCTTATGTTGCTCGAAGAATTTCTTCTGTATGAGATGCCTGATGAATTTATTGCTTCAAATGAACAGCGAGGAGCAGTACTTAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006325 GO:0006355 GO:0006950 GO:0006952 GO:0006996 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0009605 GO:0009607 GO:0009620 GO:0009791 GO:0009889 GO:0009893 GO:0009909 GO:0009911 GO:0009987 GO:0010228 GO:0010468 GO:0010556 GO:0010604 GO:0010638 GO:0016043 GO:0019219 GO:0019222 GO:0022414 GO:0023052 GO:0030154 GO:0031056 GO:0031058 GO:0031060 GO:0031062 GO:0031323 GO:0031325 GO:0031326 GO:0031399 GO:0031401 GO:0031440 GO:0031935 GO:0031937 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033043 GO:0033044 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0043954 GO:0044030 GO:0044424 GO:0044464 GO:0048366 GO:0048367 GO:0048468 GO:0048518 GO:0048522 GO:0048580 GO:0048582 GO:0048608 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0048869 GO:0050684 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051052 GO:0051094 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051276 GO:0051570 GO:0051574 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060968 GO:0061085 GO:0061087 GO:0061458 GO:0065007 GO:0070827 GO:0070828 GO:0070829 GO:0071840 GO:0080090 GO:0090436 GO:0097159 GO:0098542 GO:0099402 GO:0140110 GO:1900109 GO:1900111 GO:1900363 GO:1901363 GO:1902275 GO:1903311 GO:1903506 GO:1905269 GO:2000024 GO:2000026 GO:2000112 GO:2000241 GO:2000243 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNMT1-RFD

Protein Analysis

868

Amino Acids

98.52

Weight (kDa)

6.63

Isoelectric Point (pI)

43.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 28 - 95 4.3e-11 Rx N-terminal domain
NB-ARC PF00931 165 - 228 8.8e-10 NB-ARC domain
nSTAND_NTPase5 PF25199 177 - 284 1.2e-07 Novel STAND NTPase 5
NB-ARC PF00931 230 - 315 5.8e-16 NB-ARC domain
WHD_DRP PF23559 402 - 473 3.9e-21 Disease resistance protein Winged helix domain
LRR_14 PF23598 519 - 846 1.3e-45 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000343)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48090 AT5G48090 AT5G48090 AT5G55390 AT5G55390 AT5G55390
fragaria_vesca FvH4_3g23690 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160
malus_domestica MD03G1191000.v1.1 MD11G1207800.v1.1 MD11G1208000.v1.1
prunus_persica Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227600_v2.0.a1 Prupe.4G227700_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1
pyrus_communis pycom01g03400 pycom03g14420 pycom11g18100 pycom11g18140 pycom11g18150 pycom11g18160
rosa_chinensis RchiOBHm_Chr2g0145981 RchiOBHm_Chr4g0405241 RchiOBHm_Chr4g0405271 RchiOBHm_Chr5g0016201 RchiOBHm_Chr5g0041831 RchiOBHm_Chr5g0041871 RchiOBHm_Chr5g0041881 RchiOBHm_Chr5g0059241
rosa_laevigata RLG00000002117 RLG00000008861 RLG00000008862 RLG00000011000 RLG00000033216 RLG00000034097 RLG00000034101
rosa_multiflora Rmu_sc0000161.1_g000001 Rmu_sc0000861.1_g000040 Rmu_sc0007522.1_g000003 Rmu_sc0009443.1_g000015 Rmu_sc0022129.1_g000001 Rmu_sc0042315.1_g000001
rosa_roxburghii Rroxscaffold_1G00038660 Rroxscaffold_1G00038690 Rroxscaffold_5G00349560 Rroxscaffold_5G00349610 Rroxscaffold_6G00388370 Rroxscaffold_7G00188740
rosa_rugosa Rorug03G0199700 Rorug04G0055400 Rorug04G0055500 Rorug04G0055900 Rorug04G0056000 Rorug04G0056100 Rorug04G0056100 Rorug04G0056200 Rorug05G0195600 Rorug05G0195700 Rorug05G0195800 Rorug05G0195900 Rorug05G0196800 Rorug05G0385000
rosa_samantha Rh4AG129900 Rh4AG130100 Rh4BG124200 Rh4BG124400 Rh4BG124500 Rh4CG137200 Rh4CG137300 Rh4DG123500 Rh4DG123700 Rh5AG280800 Rh5AG281400 Rh5BG286700 Rh5BG287000 Rh5BG397800 Rh5CG318100 Rh5CG318600 Rh5CG421600 Rh5DG132300 Rh5DG132400 Rh5DG295400 Rh5DG295700 Rh7BG475300
rosa_wichuraiana Rw0G001330 Rw0G005730 Rw4G008670 Rw4G010510 Rw4G010540 Rw5G026450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 1497, 2193
AccB7I CCANNNNNTGG 1 cut(s) 217
AccIII TCCGGA 1 cut(s) 2007
AciI CCGC 5 cut(s) 5, 573, 664, 888, 2210
AclWI GGATC 2 cut(s) 2142, 2225
AcsI RAATTY 7 cut(s) 403, 629, 985, 1339, 1491, 2537, 2564
AcuI CTGAAG 4 cut(s) 149, 731, 1013, 1281
AcyI GRCGYC 2 cut(s) 1498, 2194
AfaI GTAC 8 cut(s) 88, 1196, 1561, 1611, 1624, 1656, 1706, 2598
AfiI CCNNNNNNNGG 6 cut(s) 150, 217, 1283, 1701, 2014, 2154
AflII CTTAAG 4 cut(s) 1613, 1699, 1784, 2459
AflIII ACRYGT 1 cut(s) 285
AhlI ACTAGT 1 cut(s) 1567
AjnI CCWGG 4 cut(s) 568, 908, 1830, 2196
AleI CACNNNNGTG 1 cut(s) 1796
AloI GAACNNNNNNTCC 2 cut(s) 1881, 1913
Alw21I GWGCWC 2 cut(s) 969, 1329
Alw26I GTCTC 2 cut(s) 1694, 2183
AlwI GGATC 2 cut(s) 2142, 2225
AlwNI CAGNNNCTG 1 cut(s) 301
Ama87I CYCGRG 1 cut(s) 2324
Aor13HI TCCGGA 1 cut(s) 2007
AoxI GGCC 3 cut(s) 2115, 2156, 2292
ApeKI GCWGC 4 cut(s) 177, 301, 1147, 1472
ApoI RAATTY 7 cut(s) 403, 629, 985, 1339, 1491, 2537, 2564
ArsI GACNNNNNNTTYG 2 cut(s) 576, 608
AseI ATTAAT 2 cut(s) 168, 1524
Asp700I GAANNNNTTC 2 cut(s) 2538, 2541
AspLEI GCGC 2 cut(s) 1500, 2196
AspS9I GGNCC 4 cut(s) 208, 566, 2156, 2293
AsuC2I CCSGG 1 cut(s) 1278
AsuHPI GGTGA 3 cut(s) 1496, 1810, 1966
AsuII TTCGAA 1 cut(s) 60
AvaI CYCGRG 1 cut(s) 2324
AvaII GGWCC 2 cut(s) 208, 566
BanI GGYRCC 2 cut(s) 1497, 2193
BanII GRGCYC 2 cut(s) 969, 1329
BauI CACGAG 1 cut(s) 849
BbsI GAAGAC 3 cut(s) 480, 1221, 1917
Bbv12I GWGCWC 2 cut(s) 969, 1329
BbvI GCAGC 4 cut(s) 189, 288, 1159, 1459
BccI CCATC 8 cut(s) 1182, 1232, 1360, 1364, 1757, 2072, 2147, 2435
BceAI ACGGC 2 cut(s) 1122, 1215
BciT130I CCWGG 4 cut(s) 570, 910, 1832, 2198
BcnI CCSGG 1 cut(s) 1278
BcoDI GTCTC 2 cut(s) 1694, 2183
BcuI ACTAGT 1 cut(s) 1567
BfaI CTAG 6 cut(s) 149, 501, 1568, 2237, 2241, 2420
BfmI CTRYAG 4 cut(s) 945, 1462, 1902, 2387
BfoI RGCGCY 2 cut(s) 1501, 2197
BfrI CTTAAG 4 cut(s) 1613, 1699, 1784, 2459
BglI GCCNNNNNGGC 1 cut(s) 44
BglII AGATCT 1 cut(s) 1780
BisI GCNGC 6 cut(s) 178, 302, 574, 1148, 1473, 2210
BlsI GCNGC 6 cut(s) 179, 303, 575, 1149, 1474, 2211
BmcAI AGTACT 2 cut(s) 88, 2598
Bme1390I CCNGG 5 cut(s) 570, 910, 1278, 1832, 2198
Bme18I GGWCC 2 cut(s) 208, 566
BmeT110I CYCGRG 1 cut(s) 2324
BmgT120I GGNCC 4 cut(s) 208, 566, 2156, 2293
BmiI GGNNCC 6 cut(s) 498, 577, 1455, 1499, 1899, 2195
BmrFI CCNGG 5 cut(s) 570, 910, 1278, 1832, 2198
BmrI ACTGGG 1 cut(s) 2355
BmsI GCATC 7 cut(s) 126, 226, 780, 895, 1171, 2260, 2544
BmuI ACTGGG 1 cut(s) 2355
BpiI GAAGAC 3 cut(s) 480, 1221, 1917
BpmI CTGGAG 2 cut(s) 1814, 2219
Bpu10I CCTNAGC 2 cut(s) 963, 2454
Bpu14I TTCGAA 1 cut(s) 60
BpuEI CTTGAG 3 cut(s) 434, 1651, 2309
BpuMI CCSGG 1 cut(s) 1278
BsaBI GATNNNNATC 2 cut(s) 2133, 2432
BsaHI GRCGYC 2 cut(s) 1498, 2194
BsaI GGTCTC 1 cut(s) 1694
BsaJI CCNNGG 2 cut(s) 1093, 1986
BsaWI WCCGGW 1 cut(s) 2007
BsaXI ACNNNNNCTCC 4 cut(s) 1375, 1405, 1881, 1911
Bsc4I CCNNNNNNNGG 6 cut(s) 150, 217, 1283, 1701, 2014, 2154
Bse1I ACTGG 1 cut(s) 2350
Bse3DI GCAATG 1 cut(s) 2454
Bse8I GATNNNNATC 2 cut(s) 2133, 2432
BseAI TCCGGA 1 cut(s) 2007
BseBI CCWGG 4 cut(s) 570, 910, 1832, 2198
BseDI CCNNGG 2 cut(s) 1093, 1986
BseGI GGATG 4 cut(s) 1375, 2064, 2275, 2446
BseJI GATNNNNATC 2 cut(s) 2133, 2432
BseLI CCNNNNNNNGG 6 cut(s) 150, 217, 1283, 1701, 2014, 2154
BseMI GCAATG 1 cut(s) 2454
BseMII CTCAG 7 cut(s) 272, 308, 408, 715, 954, 1706, 2445
BseNI ACTGG 1 cut(s) 2350
BseRI GAGGAG 3 cut(s) 509, 2151, 2604
BseXI GCAGC 4 cut(s) 189, 288, 1159, 1459
BseYI CCCAGC 2 cut(s) 1304, 2223
BsgI GTGCAG 1 cut(s) 287
Bsh1285I CGRYCG 1 cut(s) 82
BshFI GGCC 3 cut(s) 2117, 2158, 2294
BshNI GGYRCC 2 cut(s) 1497, 2193
BsiEI CGRYCG 1 cut(s) 82
BsiHKAI GWGCWC 2 cut(s) 969, 1329
BsiHKCI CYCGRG 1 cut(s) 2324
BsiSI CCGG 3 cut(s) 579, 1277, 2008
BslFI GGGAC 3 cut(s) 167, 646, 2004
BslI CCNNNNNNNGG 6 cut(s) 150, 217, 1283, 1701, 2014, 2154
BsmAI GTCTC 2 cut(s) 1694, 2183
BsmBI CGTCTC 1 cut(s) 2183
BsmFI GGGAC 3 cut(s) 167, 646, 2004
BsmI GAATGC 2 cut(s) 189, 621
BsnI GGCC 3 cut(s) 2117, 2158, 2294
Bso31I GGTCTC 1 cut(s) 1694
BsoBI CYCGRG 1 cut(s) 2324
Bsp119I TTCGAA 1 cut(s) 60
Bsp1286I GDGCHC 2 cut(s) 969, 1329
Bsp13I TCCGGA 1 cut(s) 2007
Bsp1407I TGTACA 1 cut(s) 1622
Bsp143I GATC 8 cut(s) 685, 760, 1780, 2026, 2102, 2134, 2217, 2258
BspACI CCGC 5 cut(s) 5, 573, 664, 888, 2210
BspANI GGCC 3 cut(s) 2117, 2158, 2294
BspCNI CTCAG 7 cut(s) 271, 307, 407, 714, 955, 1707, 2446
BspEI TCCGGA 1 cut(s) 2007
BspLI GGNNCC 6 cut(s) 498, 577, 1455, 1499, 1899, 2195
BspMAI CTGCAG 3 cut(s) 949, 1466, 1906
BspPI GGATC 2 cut(s) 2142, 2225
BspT104I TTCGAA 1 cut(s) 60
BspT107I GGYRCC 2 cut(s) 1497, 2193
BspTI CTTAAG 4 cut(s) 1613, 1699, 1784, 2459
BspTNI GGTCTC 1 cut(s) 1694
BsrDI GCAATG 1 cut(s) 2454
BsrGI TGTACA 1 cut(s) 1622
BsrI ACTGG 1 cut(s) 2350
BssECI CCNNGG 2 cut(s) 1093, 1986
BssMI GATC 8 cut(s) 685, 760, 1780, 2026, 2102, 2134, 2217, 2258
BssNI GRCGYC 2 cut(s) 1498, 2194
BssSI CACGAG 1 cut(s) 849
BssT1I CCWWGG 2 cut(s) 1093, 1986
Bst2BI CACGAG 1 cut(s) 849
Bst2UI CCWGG 4 cut(s) 570, 910, 1832, 2198
Bst4CI ACNGT 5 cut(s) 522, 592, 1447, 1995, 2055
Bst6I CTCTTC 4 cut(s) 156, 198, 966, 2041
BstACI GRCGYC 2 cut(s) 1498, 2194
BstAFI CTTAAG 4 cut(s) 1613, 1699, 1784, 2459
BstAUI TGTACA 1 cut(s) 1622
BstBI TTCGAA 1 cut(s) 60
BstC8I GCNNGC 5 cut(s) 118, 141, 1044, 1581, 1906
BstDEI CTNAG 9 cut(s) 258, 294, 394, 701, 963, 1715, 1777, 2454, 2600
BstEII GGTNACC 1 cut(s) 1798
BstF5I GGATG 4 cut(s) 1375, 2064, 2275, 2446
BstH2I RGCGCY 2 cut(s) 1501, 2197
BstHHI GCGC 2 cut(s) 1500, 2196
BstKTI GATC 8 cut(s) 688, 763, 1783, 2029, 2105, 2137, 2220, 2261
BstMAI GTCTC 2 cut(s) 1694, 2183
BstMBI GATC 8 cut(s) 685, 760, 1780, 2026, 2102, 2134, 2217, 2258
BstMCI CGRYCG 1 cut(s) 82
BstMWI GCNNNNNNNGC 6 cut(s) 11, 44, 579, 1025, 2246, 2464
BstNI CCWGG 4 cut(s) 570, 910, 1832, 2198
BstNSI RCATGY 3 cut(s) 289, 1162, 1207
BstPI GGTNACC 1 cut(s) 1798
BstSCI CCNGG 5 cut(s) 568, 908, 1276, 1830, 2196
BstSFI CTRYAG 4 cut(s) 945, 1462, 1902, 2387
BstV1I GCAGC 4 cut(s) 189, 288, 1159, 1459
BstV2I GAAGAC 3 cut(s) 480, 1221, 1917
BstX2I RGATCY 2 cut(s) 1780, 2217
BstXI CCANNNNNNTGG 1 cut(s) 1071
BstYI RGATCY 2 cut(s) 1780, 2217
BsuRI GGCC 3 cut(s) 2117, 2158, 2294
BtgZI GCGATG 1 cut(s) 62
BtsCI GGATG 4 cut(s) 1375, 2064, 2275, 2446
BtsI GCAGTG 1 cut(s) 2219
BtsIMutI CAGTG 3 cut(s) 588, 1443, 2219
Cac8I GCNNGC 5 cut(s) 118, 141, 1044, 1581, 1906
CaiI CAGNNNCTG 1 cut(s) 301
CfoI GCGC 2 cut(s) 1500, 2196
Cfr13I GGNCC 4 cut(s) 208, 566, 2156, 2293
CseI GACGC 1 cut(s) 1829
Csp6I GTAC 8 cut(s) 87, 1195, 1560, 1610, 1623, 1655, 1705, 2597
CviAII CATG 8 cut(s) 98, 253, 286, 875, 1159, 1204, 1349, 2042
CviQI GTAC 8 cut(s) 87, 1195, 1560, 1610, 1623, 1655, 1705, 2597
DdeI CTNAG 9 cut(s) 258, 294, 394, 701, 963, 1715, 1777, 2454, 2600
DinI GGCGCC 2 cut(s) 1499, 2195
DpnI GATC 8 cut(s) 687, 762, 1782, 2028, 2104, 2136, 2219, 2260
DpnII GATC 8 cut(s) 685, 760, 1780, 2026, 2102, 2134, 2217, 2258
DraI TTTAAA 2 cut(s) 1662, 2253
Eam1104I CTCTTC 4 cut(s) 156, 198, 966, 2041
EarI CTCTTC 4 cut(s) 156, 198, 966, 2041
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 2 cut(s) 967, 1327
Eco130I CCWWGG 2 cut(s) 1093, 1986
Eco24I GRGCYC 2 cut(s) 969, 1329
Eco31I GGTCTC 1 cut(s) 1694
Eco47I GGWCC 2 cut(s) 208, 566
Eco53kI GAGCTC 2 cut(s) 967, 1327
Eco57I CTGAAG 4 cut(s) 149, 731, 1013, 1281
Eco88I CYCGRG 1 cut(s) 2324
Eco91I GGTNACC 1 cut(s) 1798
EcoICRI GAGCTC 2 cut(s) 967, 1327
EcoO109I RGGNCCY 1 cut(s) 2293
EcoO65I GGTNACC 1 cut(s) 1798
EcoRI GAATTC 1 cut(s) 403
EcoRII CCWGG 4 cut(s) 568, 908, 1830, 2196
EcoT14I CCWWGG 2 cut(s) 1093, 1986
EcoT22I ATGCAT 1 cut(s) 1164
EcoT38I GRGCYC 2 cut(s) 969, 1329
EgeI GGCGCC 2 cut(s) 1499, 2195
EheI GGCGCC 2 cut(s) 1499, 2195
ErhI CCWWGG 2 cut(s) 1093, 1986
Esp3I CGTCTC 1 cut(s) 2183
FaeI CATG 8 cut(s) 101, 256, 289, 878, 1162, 1207, 1352, 2045
FaqI GGGAC 3 cut(s) 167, 646, 2004
FatI CATG 8 cut(s) 97, 252, 285, 874, 1158, 1203, 1348, 2041
FauI CCCGC 1 cut(s) 671
FauNDI CATATG 1 cut(s) 2139
Fnu4HI GCNGC 6 cut(s) 178, 302, 574, 1148, 1473, 2210
FokI GGATG 4 cut(s) 1382, 2051, 2282, 2453
FriOI GRGCYC 2 cut(s) 969, 1329
Fsp4HI GCNGC 6 cut(s) 178, 302, 574, 1148, 1473, 2210
FspBI CTAG 6 cut(s) 149, 501, 1568, 2237, 2241, 2420
GlaI GCGC 2 cut(s) 1499, 2195
GluI GCNGC 6 cut(s) 178, 302, 574, 1148, 1473, 2210
GsaI CCCAGC 2 cut(s) 1308, 2227
GsuI CTGGAG 2 cut(s) 1814, 2219
HaeII RGCGCY 2 cut(s) 1501, 2197
HaeIII GGCC 3 cut(s) 2117, 2158, 2294
HapII CCGG 3 cut(s) 579, 1277, 2008
HgaI GACGC 1 cut(s) 1829
HhaI GCGC 2 cut(s) 1500, 2196
Hin1I GRCGYC 2 cut(s) 1498, 2194
Hin1II CATG 8 cut(s) 101, 256, 289, 878, 1162, 1207, 1352, 2045
Hin6I GCGC 2 cut(s) 1498, 2194
HinP1I GCGC 2 cut(s) 1498, 2194
HincII GTYRAC 1 cut(s) 2335
HindII GTYRAC 1 cut(s) 2335
HinfI GANTC 6 cut(s) 155, 262, 857, 1555, 2203, 2276
HpaII CCGG 3 cut(s) 579, 1277, 2008
HphI GGTGA 3 cut(s) 1496, 1810, 1966
Hpy166II GTNNAC 3 cut(s) 959, 1655, 2335
Hpy8I GTNNAC 3 cut(s) 959, 1655, 2335
Hpy99I CGWCG 1 cut(s) 311
HpyCH4III ACNGT 5 cut(s) 522, 592, 1447, 1995, 2055
HpyCH4IV ACGT 2 cut(s) 309, 2176
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 44, 579, 1025, 2246, 2464
HpyF3I CTNAG 9 cut(s) 258, 294, 394, 701, 963, 1715, 1777, 2454, 2600
HpySE526I ACGT 2 cut(s) 309, 2176
Hsp92I GRCGYC 2 cut(s) 1498, 2194
Hsp92II CATG 8 cut(s) 101, 256, 289, 878, 1162, 1207, 1352, 2045
HspAI GCGC 2 cut(s) 1498, 2194
KasI GGCGCC 2 cut(s) 1497, 2193
Kpn2I TCCGGA 1 cut(s) 2007
Kzo9I GATC 8 cut(s) 685, 760, 1780, 2026, 2102, 2134, 2217, 2258
LmnI GCTCC 6 cut(s) 496, 581, 1383, 1916, 2444, 2591
Lsp1109I GCAGC 4 cut(s) 189, 288, 1159, 1459
LweI GCATC 7 cut(s) 126, 226, 780, 895, 1171, 2260, 2544
MaeI CTAG 6 cut(s) 149, 501, 1568, 2237, 2241, 2420
MaeII ACGT 2 cut(s) 309, 2176
MaeIII GTNAC 7 cut(s) 827, 1080, 1154, 1232, 1798, 1867, 2496
MalI GATC 8 cut(s) 687, 762, 1782, 2028, 2104, 2136, 2219, 2260
MboI GATC 8 cut(s) 685, 760, 1780, 2026, 2102, 2134, 2217, 2258
MfeI CAATTG 1 cut(s) 1065
MflI RGATCY 2 cut(s) 1780, 2217
MhlI GDGCHC 2 cut(s) 969, 1329
Mly113I GGCGCC 2 cut(s) 1498, 2194
MlyI GAGTC 3 cut(s) 149, 866, 2212
MmeI TCCRAC 2 cut(s) 99, 507
Mph1103I ATGCAT 1 cut(s) 1164
MroI TCCGGA 1 cut(s) 2007
MroXI GAANNNNTTC 2 cut(s) 2538, 2541
MslI CAYNNNNRTG 4 cut(s) 31, 102, 1482, 1796
MspCI CTTAAG 4 cut(s) 1613, 1699, 1784, 2459
MspI CCGG 3 cut(s) 579, 1277, 2008
MspR9I CCNGG 5 cut(s) 570, 910, 1278, 1832, 2198
MunI CAATTG 1 cut(s) 1065
Mva1269I GAATGC 2 cut(s) 189, 621
MvaI CCWGG 4 cut(s) 570, 910, 1832, 2198
MwoI GCNNNNNNNGC 6 cut(s) 11, 44, 579, 1025, 2246, 2464
NarI GGCGCC 2 cut(s) 1498, 2194
NciI CCSGG 1 cut(s) 1278
NdeI CATATG 1 cut(s) 2139
NdeII GATC 8 cut(s) 685, 760, 1780, 2026, 2102, 2134, 2217, 2258
NlaIII CATG 8 cut(s) 101, 256, 289, 878, 1162, 1207, 1352, 2045
NlaIV GGNNCC 6 cut(s) 498, 577, 1455, 1499, 1899, 2195
NmeAIII GCCGAG 2 cut(s) 64, 2143
NmuCI GTSAC 2 cut(s) 1232, 1798
NsiI ATGCAT 1 cut(s) 1164
NspI RCATGY 3 cut(s) 289, 1162, 1207
NspV TTCGAA 1 cut(s) 60
OliI CACNNNNGTG 1 cut(s) 1796
PaeR7I CTCGAG 1 cut(s) 2324
PciI ACATGT 1 cut(s) 285
PctI GAATGC 2 cut(s) 189, 621
PdmI GAANNNNTTC 2 cut(s) 2538, 2541
PfeI GAWTC 3 cut(s) 262, 1555, 2276
PflMI CCANNNNNTGG 1 cut(s) 217
PfoI TCCNGGA 1 cut(s) 1830
PkrI GCNGC 6 cut(s) 179, 303, 575, 1149, 1474, 2211
PleI GAGTC 3 cut(s) 149, 865, 2211
PluTI GGCGCC 2 cut(s) 1501, 2197
PpsI GAGTC 3 cut(s) 149, 865, 2211
PscI ACATGT 1 cut(s) 285
PshBI ATTAAT 2 cut(s) 168, 1524
Psp124BI GAGCTC 2 cut(s) 969, 1329
Psp6I CCWGG 4 cut(s) 568, 908, 1830, 2196
PspEI GGTNACC 1 cut(s) 1798
PspFI CCCAGC 2 cut(s) 1304, 2223
PspGI CCWGG 4 cut(s) 568, 908, 1830, 2196
PspN4I GGNNCC 6 cut(s) 498, 577, 1455, 1499, 1899, 2195
PspPI GGNCC 4 cut(s) 208, 566, 2156, 2293
PspXI VCTCGAGB 1 cut(s) 2324
PstI CTGCAG 3 cut(s) 949, 1466, 1906
PstNI CAGNNNCTG 1 cut(s) 301
PsuI RGATCY 2 cut(s) 1780, 2217
RsaI GTAC 8 cut(s) 88, 1196, 1561, 1611, 1624, 1656, 1706, 2598
RsaNI GTAC 8 cut(s) 87, 1195, 1560, 1610, 1623, 1655, 1705, 2597
RseI CAYNNNNRTG 4 cut(s) 31, 102, 1482, 1796
SacI GAGCTC 2 cut(s) 969, 1329
SatI GCNGC 6 cut(s) 178, 302, 574, 1148, 1473, 2210
Sau3AI GATC 8 cut(s) 685, 760, 1780, 2026, 2102, 2134, 2217, 2258
Sau96I GGNCC 4 cut(s) 208, 566, 2156, 2293
SbfI CCTGCAGG 1 cut(s) 1906
ScaI AGTACT 2 cut(s) 88, 2598
SchI GAGTC 3 cut(s) 149, 866, 2212
ScrFI CCNGG 5 cut(s) 570, 910, 1278, 1832, 2198
SdaI CCTGCAGG 1 cut(s) 1906
SduI GDGCHC 2 cut(s) 969, 1329
SfaNI GCATC 7 cut(s) 126, 226, 780, 895, 1171, 2260, 2544
SfcI CTRYAG 4 cut(s) 945, 1462, 1902, 2387
SfoI GGCGCC 2 cut(s) 1499, 2195
Sfr274I CTCGAG 1 cut(s) 2324
SfuI TTCGAA 1 cut(s) 60
SinI GGWCC 2 cut(s) 208, 566
SlaI CTCGAG 1 cut(s) 2324
SmiMI CAYNNNNRTG 4 cut(s) 31, 102, 1482, 1796
SmlI CTYRAG 8 cut(s) 413, 1613, 1630, 1699, 1784, 2288, 2324, 2459
SmoI CTYRAG 8 cut(s) 413, 1613, 1630, 1699, 1784, 2288, 2324, 2459
SpeI ACTAGT 1 cut(s) 1567
Sse8387I CCTGCAGG 1 cut(s) 1906
SsiI CCGC 5 cut(s) 5, 573, 664, 888, 2210
SspDI GGCGCC 2 cut(s) 1497, 2193
SspI AATATT 1 cut(s) 1522
SspMI CTAG 6 cut(s) 149, 501, 1568, 2237, 2241, 2420
SstI GAGCTC 2 cut(s) 969, 1329
StyD4I CCNGG 5 cut(s) 568, 908, 1276, 1830, 2196
StyI CCWWGG 2 cut(s) 1093, 1986
TaaI ACNGT 5 cut(s) 522, 592, 1447, 1995, 2055
TaiI ACGT 2 cut(s) 312, 2179
TaqII GACCGA 1 cut(s) 96
TatI WGTACW 3 cut(s) 86, 1622, 2596
TauI GCSGC 2 cut(s) 576, 2212
TfiI GAWTC 3 cut(s) 262, 1555, 2276
TscAI CASTG 3 cut(s) 595, 1450, 2219
TseFI GTSAC 2 cut(s) 1232, 1798
TseI GCWGC 4 cut(s) 177, 301, 1147, 1472
Tsp45I GTSAC 2 cut(s) 1232, 1798
TspRI CASTG 3 cut(s) 595, 1450, 2219
Van91I CCANNNNNTGG 1 cut(s) 217
Vha464I CTTAAG 4 cut(s) 1613, 1699, 1784, 2459
VpaK11BI GGWCC 2 cut(s) 208, 566
VspI ATTAAT 2 cut(s) 168, 1524
XapI RAATTY 7 cut(s) 403, 629, 985, 1339, 1491, 2537, 2564
XceI RCATGY 3 cut(s) 289, 1162, 1207
XcmI CCANNNNNNNNNTGG 2 cut(s) 523, 1363
XhoI CTCGAG 1 cut(s) 2324
XmnI GAANNNNTTC 2 cut(s) 2538, 2541
XspI CTAG 6 cut(s) 149, 501, 1568, 2237, 2241, 2420
ZrmI AGTACT 2 cut(s) 88, 2598
Zsp2I ATGCAT 1 cut(s) 1164
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.