Rh5DG295400

metal ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000343
Physical Location & Seq
Reverse (-)
37253633 .. 37265692
12060 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG295400.1

Sequence Viewer

Length: 2463 bp
ATGGAATCGTCAGATGATGAGGCTGAGGCAATGCAGCTTTCGGTTTCGAATTACTACTTTGTAGATGGCAAGGAAGATCCTATCTCCTTTCATGTACTGCCCATTCAATGGAGTGAGGGTGATAGGCAGGATGGGAAGGAAGCAGTGTTGTTTCTGCATGGCAGTGCCGACAATGGGCTCGAGAAGATTTACAAACCTGTTGTTGCTTGGAAGTTTGATCTTTCGAATGTGAAGCCCGAGATTGCAGTGCTTTCCAAGGAGAATGATTGGATTCTGCTCCAGAAGCCGAGGAGGAGCTATGAGGGGATCGTAAGGACTGTCTTGATAACTGTGCAGTGCCTTAGTTATGCAATGTGGAATGCTGAGGCGTCCGGAAAAGCTGTGTGGGATCACTTGTCTAAAGCGTTCAGCATGTATGAGAACAGGCCTTCGCAGCATGATTTGGGTGATCACAGGAGTCTGATTAGTGAAGCTCTAAAGCGAGATGATGCCTTGGCAAAGTCGAAGTTTATAATTGACTTTCTTAAAGAAGAGCCACCCACAAAGAGGAAACTATCTGATGAGGATATTCAAGCTACAGCAAAGCTTGGATTTATAGTTGATGATGCGGAAGATTATATAATTGATGATGAAGATGAGTCAGATGATGATGATGATGATAACCTGTTTGATTCTGTTTGTGCCTTTTGTGACAATGGTGGCAAGCTGTTATGTTGTGAAGGGAGATGCCTAAGGTCCTTCCATCCCACGGTAGAGGACGGCGAGGACTCAATGTGTGAATCTCTTGGATTCACTCGGGAGGAAGTAGATGCAATGGCAAGTTTCTTCTGTAAGAACTGTCAATATAAGCAGCATCAGTGCTTTGCTTGTGGGAAGTTAGGCTCCTCAGACAAATCTTTGGGTGCAAAGGTCTTCCCCTGTGTTTCTGCAACCTGTGGTCAATTTTACCATCCTCATTGTGTTGCACAACTAATTTATCAAGACAATGGAGTTTCTGCTGAAGAACTGGAGAAAAATATCTCTCTGGGGGAGTCTTTTACGTGTCCGATTCATAAGTGCTGTGTTTGTAAGCAAGGAGAGAATAAGAAGGATCCCGAGTTACGGTTTGCTGTGTGCAGGCGTTGTCCTAAATCCTATCACAGGAAATGCTTGCCCAGTGAGATTCGTTTTGAGAAAACAGAGGAAGATGATGAGGAAGAGATAGAGGATGAAGAAGAAATAGAAACAAGAGCTTGGGAAGGTCTATTACCTAATCGCATACTAATATATTGCACAGAACACGAAATAGATGAAGAAATTGGAACTCCAATAAGGGACCATGTTAAATTTCCCGATGTTGACGGAAAGAAGACTAGTATTGTGAAAAGGAAGACTGCTTTTGAACAGAAAAAGAGAAGACTGACATCAGAGTCCCTTGTGGTCAGAGATAATTCTCTGTCGAAGAAAAGAAAGCTTACCTCAGAGGGATTTTATAGTGGAAGAACTGCACCTCCAATATCTAGGCAGAAATTGAATTCGGGTGAAAAAGTTGGCAGTAGCAGAAATACTGAAAAAGTACCTTCTCGGTTAGACGTATCAAGAAAGGTGATGGTTAATAAGACATTGAAAAAGGAAGTTCAGACTAGCGTGGAAAAGAAGACCTCGTTGGGTAATAGACTGTTTACCTACATAAAGGGATCTGGGTCAGTAAAGTTTGGGAAGCAGGATACACCTGATGATGCTGAACTAAATTCTAAAACGAGTGCAAAAATTGATCCTACATCAAGAACATTGAGTGCGGAAGCTTCCTTAGATCCTGCTAGAGAAAGGAGATTACATGCTTTAATGAAAGACGCTGCTTCATCAATAAGATTGGAAGATGTTATAGAAAAGCACAAAGTTCCATCCACTCATGCATCCTCAAATAAATATGCTGTAGAAAGGAACATAACACGAGGAAAGGTGGAAGGCTCAGTTGAGGCTGTTCGAACCGCATTAAAGAAACTACAGGAAGGCTGCAGTACTGAAGATGCAGAGGCTGTCTGTGCTCCTGAGATTCTTTCCCAGATTTATAAGTGGAAGAGCAAGCTTAGAGTATATCTTGCACCTTTCTTGCATGGAAAGCGGTACACGTCCTTCGGTCGCCATTTCACAAAGGTGGAAAAACTCGAACAGATTGCTGATAGGCTCCATTCGTACGTACAGAATGGAGACACGATAGTGGACTTCTGTTGTGGCTCTAATGATTTCAGCATCGTAATGAAGAAAAAGCTTGAGGAGACAGGGAAGAAGTGCTATTTTAGAAATTATGACATTATTCAACCAAAGAATGATTTCTGTTTTGAGAAGAGAGATTGGATGACTGTTCAAAAAGGTGAATTACCGGATAGTAGTAAATTGGTAAGAAATGGAGGCTTGGTTATATTTTACATCAAGTTGTTTGGATTCCTTAAGCCTTGTGCTAATTGTTTCACTATTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006325 GO:0006355 GO:0006950 GO:0006952 GO:0006996 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0009605 GO:0009607 GO:0009620 GO:0009791 GO:0009889 GO:0009893 GO:0009909 GO:0009911 GO:0009987 GO:0010228 GO:0010468 GO:0010556 GO:0010604 GO:0010638 GO:0016043 GO:0019219 GO:0019222 GO:0022414 GO:0023052 GO:0030154 GO:0031056 GO:0031058 GO:0031060 GO:0031062 GO:0031323 GO:0031325 GO:0031326 GO:0031399 GO:0031401 GO:0031440 GO:0031935 GO:0031937 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033043 GO:0033044 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0043954 GO:0044030 GO:0044424 GO:0044464 GO:0048366 GO:0048367 GO:0048468 GO:0048518 GO:0048522 GO:0048580 GO:0048582 GO:0048608 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0048869 GO:0050684 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051052 GO:0051094 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051276 GO:0051570 GO:0051574 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060968 GO:0061085 GO:0061087 GO:0061458 GO:0065007 GO:0070827 GO:0070828 GO:0070829 GO:0071840 GO:0080090 GO:0090436 GO:0097159 GO:0098542 GO:0099402 GO:0140110 GO:1900109 GO:1900111 GO:1900363 GO:1901363 GO:1902275 GO:1903311 GO:1903506 GO:1905269 GO:2000024 GO:2000026 GO:2000112 GO:2000241 GO:2000243 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNMT1-RFD

Protein Analysis

820

Amino Acids

93.0

Weight (kDa)

6.05

Isoelectric Point (pI)

42.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNMT1-RFD PF12047 13 - 111 1e-06 Cytosine specific DNA methyltransferase replication foci domain
PHD_NSD PF22908 283 - 351 1.8e-19 Histone-lysine N-methyltransferase NSD-like, PHD zinc finger
Mtase_EDM2 PF26055 726 - 794 8.3e-33 EDM2 methyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000343)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48090 AT5G48090 AT5G48090 AT5G55390 AT5G55390 AT5G55390
fragaria_vesca FvH4_3g23690 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160
malus_domestica MD03G1191000.v1.1 MD11G1207800.v1.1 MD11G1208000.v1.1
prunus_persica Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227600_v2.0.a1 Prupe.4G227700_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1
pyrus_communis pycom01g03400 pycom03g14420 pycom11g18100 pycom11g18140 pycom11g18150 pycom11g18160
rosa_chinensis RchiOBHm_Chr2g0145981 RchiOBHm_Chr4g0405241 RchiOBHm_Chr4g0405271 RchiOBHm_Chr5g0016201 RchiOBHm_Chr5g0041831 RchiOBHm_Chr5g0041871 RchiOBHm_Chr5g0041881 RchiOBHm_Chr5g0059241
rosa_laevigata RLG00000002117 RLG00000008861 RLG00000008862 RLG00000011000 RLG00000033216 RLG00000034097 RLG00000034101
rosa_multiflora Rmu_sc0000161.1_g000001 Rmu_sc0000861.1_g000040 Rmu_sc0007522.1_g000003 Rmu_sc0009443.1_g000015 Rmu_sc0022129.1_g000001 Rmu_sc0042315.1_g000001
rosa_roxburghii Rroxscaffold_1G00038660 Rroxscaffold_1G00038690 Rroxscaffold_5G00349560 Rroxscaffold_5G00349610 Rroxscaffold_6G00388370 Rroxscaffold_7G00188740
rosa_rugosa Rorug03G0199700 Rorug04G0055400 Rorug04G0055500 Rorug04G0055900 Rorug04G0056000 Rorug04G0056100 Rorug04G0056100 Rorug04G0056200 Rorug05G0195600 Rorug05G0195700 Rorug05G0195800 Rorug05G0195900 Rorug05G0196800 Rorug05G0385000
rosa_samantha Rh4AG129900 Rh4AG130100 Rh4BG124200 Rh4BG124400 Rh4BG124500 Rh4CG137200 Rh4CG137300 Rh4DG123500 Rh4DG123700 Rh5AG280800 Rh5AG281400 Rh5BG286700 Rh5BG287000 Rh5BG397800 Rh5CG318100 Rh5CG318600 Rh5CG421600 Rh5DG132300 Rh5DG132400 Rh5DG295400 Rh5DG295700 Rh7BG475300
rosa_wichuraiana Rw0G001330 Rw0G005730 Rw4G008670 Rw4G010510 Rw4G010540 Rw5G026450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 512, 2052
AasI GACNNNNNNGTC 1 cut(s) 1408
AccB7I CCANNNNNTGG 1 cut(s) 108
AccIII TCCGGA 1 cut(s) 371
AciI CCGC 4 cut(s) 608, 1778, 1971, 2104
AclWI GGATC 8 cut(s) 71, 314, 396, 1085, 1098, 1684, 1748, 1787
AcsI RAATTY 3 cut(s) 1325, 1513, 1729
AcuI CTGAAG 2 cut(s) 1020, 2025
AcyI GRCGYC 1 cut(s) 368
AfaI GTAC 6 cut(s) 96, 1557, 2002, 2108, 2177, 2181
AfiI CCNNNNNNNGG 6 cut(s) 108, 174, 546, 748, 1101, 1140
AflII CTTAAG 1 cut(s) 2429
AflIII ACRYGT 2 cut(s) 1040, 2109
AgsI TTSAA 7 cut(s) 107, 572, 1382, 1513, 1606, 2300, 2348
AhlI ACTAGT 1 cut(s) 1352
AjiI CACGTC 1 cut(s) 2112
AjuI GAANNNNNNNTTGG 2 cut(s) 1628, 1660
AleI CACNNNNGTG 2 cut(s) 2135, 2198
AloI GAACNNNNNNTCC 2 cut(s) 1474, 1506
Alw21I GWGCWC 1 cut(s) 2029
Alw26I GTCTC 2 cut(s) 2184, 2252
AlwI GGATC 8 cut(s) 71, 314, 396, 1085, 1098, 1684, 1748, 1787
AlwNI CAGNNNCTG 1 cut(s) 2018
Ama87I CYCGRG 4 cut(s) 179, 236, 795, 1094
Aor13HI TCCGGA 1 cut(s) 371
AoxI GGCC 1 cut(s) 425
ApeKI GCWGC 5 cut(s) 34, 433, 850, 1835, 1995
ApoI RAATTY 3 cut(s) 1325, 1513, 1729
Asp700I GAANNNNTTC 1 cut(s) 2312
AspS9I GGNCC 2 cut(s) 735, 1315
AsuHPI GGTGA 5 cut(s) 131, 458, 1532, 1597, 2366
AsuII TTCGAA 3 cut(s) 47, 224, 1966
AvaI CYCGRG 4 cut(s) 179, 236, 795, 1094
AvaII GGWCC 2 cut(s) 735, 1315
AxyI CCTNAGG 1 cut(s) 731
BamHI GGATCC 1 cut(s) 1090
BanII GRGCYC 1 cut(s) 180
BarI GAAGNNNNNNTAC 4 cut(s) 1230, 1262, 2099, 2131
BauI CACGAG 1 cut(s) 1932
BbsI GAAGAC 5 cut(s) 904, 1355, 1376, 1402, 1643
Bbv12I GWGCWC 1 cut(s) 2029
BbvCI CCTCAGC 2 cut(s) 24, 363
BbvI GCAGC 5 cut(s) 46, 445, 862, 1822, 1982
BccI CCATC 6 cut(s) 59, 125, 750, 957, 1582, 1891
BceAI ACGGC 1 cut(s) 775
BcgI CGANNNNNNTGC 2 cut(s) 2138, 2172
BciVI GTATCC 1 cut(s) 1699
BclI TGATCA 1 cut(s) 448
BcoDI GTCTC 2 cut(s) 2184, 2252
BcuI ACTAGT 1 cut(s) 1352
BfaI CTAG 4 cut(s) 1353, 1500, 1623, 1800
BfmI CTRYAG 4 cut(s) 576, 1914, 1985, 1996
BfrI CTTAAG 1 cut(s) 2429
BfuI GTATCC 1 cut(s) 1699
BisI GCNGC 5 cut(s) 35, 434, 851, 1836, 1996
BlsI GCNGC 5 cut(s) 36, 435, 852, 1837, 1997
BmcAI AGTACT 1 cut(s) 2002
Bme18I GGWCC 2 cut(s) 735, 1315
BmeT110I CYCGRG 4 cut(s) 179, 236, 795, 1094
BmgBI CACGTC 1 cut(s) 2112
BmgT120I GGNCC 2 cut(s) 735, 1315
BmiI GGNNCC 4 cut(s) 883, 1092, 1316, 2168
BmrI ACTGGG 1 cut(s) 1149
BmsI GCATC 9 cut(s) 478, 595, 716, 799, 862, 1708, 1904, 1999, 2241
BmuI ACTGGG 1 cut(s) 1149
BpiI GAAGAC 5 cut(s) 904, 1355, 1376, 1402, 1643
BpmI CTGGAG 2 cut(s) 263, 1028
Bpu10I CCTNAGC 2 cut(s) 24, 363
Bpu14I TTCGAA 3 cut(s) 47, 224, 1966
BpuEI CTTGAG 1 cut(s) 2273
BsaAI YACGTR 2 cut(s) 1041, 2179
BsaHI GRCGYC 1 cut(s) 368
BsaJI CCNNGG 4 cut(s) 255, 287, 492, 747
BsaWI WCCGGW 2 cut(s) 371, 2362
BsaXI ACNNNNNCTCC 6 cut(s) 103, 133, 1474, 1504, 2382, 2412
Bsc4I CCNNNNNNNGG 6 cut(s) 108, 174, 546, 748, 1101, 1140
Bse1I ACTGG 2 cut(s) 1011, 1155
Bse21I CCTNAGG 1 cut(s) 731
Bse3DI GCAATG 3 cut(s) 36, 357, 819
BseAI TCCGGA 1 cut(s) 371
BseDI CCNNGG 4 cut(s) 255, 287, 492, 747
BseGI GGATG 7 cut(s) 136, 742, 949, 1213, 1883, 1895, 2343
BseLI CCNNNNNNNGG 6 cut(s) 108, 174, 546, 748, 1101, 1140
BseMI GCAATG 3 cut(s) 36, 357, 819
BseMII CTCAG 6 cut(s) 15, 354, 900, 1473, 1965, 2022
BseNI ACTGG 2 cut(s) 1011, 1155
BseRI GAGGAG 4 cut(s) 304, 307, 874, 2270
BseXI GCAGC 5 cut(s) 46, 445, 862, 1822, 1982
BsgI GTGCAG 3 cut(s) 353, 1135, 1470
Bsh1285I CGRYCG 1 cut(s) 2122
BshFI GGCC 1 cut(s) 427
BsiEI CGRYCG 1 cut(s) 2122
BsiHKAI GWGCWC 1 cut(s) 2029
BsiHKCI CYCGRG 4 cut(s) 179, 236, 795, 1094
BsiSI CCGG 2 cut(s) 372, 2363
BsiWI CGTACG 1 cut(s) 2175
BslFI GGGAC 2 cut(s) 1328, 1396
BslI CCNNNNNNNGG 6 cut(s) 108, 174, 546, 748, 1101, 1140
BsmAI GTCTC 2 cut(s) 2184, 2252
BsmFI GGGAC 2 cut(s) 1328, 1396
BsmI GAATGC 1 cut(s) 364
BsnI GGCC 1 cut(s) 427
BsoBI CYCGRG 4 cut(s) 179, 236, 795, 1094
Bsp119I TTCGAA 3 cut(s) 47, 224, 1966
Bsp1286I GDGCHC 2 cut(s) 180, 2029
Bsp13I TCCGGA 1 cut(s) 371
Bsp143I GATC 9 cut(s) 76, 217, 306, 388, 448, 1090, 1676, 1753, 1792
BspACI CCGC 4 cut(s) 608, 1778, 1971, 2104
BspANI GGCC 1 cut(s) 427
BspCNI CTCAG 6 cut(s) 16, 355, 899, 1472, 1964, 2023
BspEI TCCGGA 1 cut(s) 371
BspLI GGNNCC 4 cut(s) 883, 1092, 1316, 2168
BspMAI CTGCAG 1 cut(s) 2000
BspPI GGATC 8 cut(s) 71, 314, 396, 1085, 1098, 1684, 1748, 1787
BspQI GCTCTTC 2 cut(s) 525, 2054
BspT104I TTCGAA 3 cut(s) 47, 224, 1966
BspTI CTTAAG 1 cut(s) 2429
BsrDI GCAATG 3 cut(s) 36, 357, 819
BsrI ACTGG 2 cut(s) 1011, 1155
BssECI CCNNGG 4 cut(s) 255, 287, 492, 747
BssMI GATC 9 cut(s) 76, 217, 306, 388, 448, 1090, 1676, 1753, 1792
BssNI GRCGYC 1 cut(s) 368
BssSI CACGAG 1 cut(s) 1932
BssT1I CCWWGG 2 cut(s) 255, 492
Bst2BI CACGAG 1 cut(s) 1932
Bst4CI ACNGT 7 cut(s) 319, 331, 751, 839, 1104, 1659, 2344
Bst6I CTCTTC 4 cut(s) 525, 1191, 2054, 2321
BstACI GRCGYC 1 cut(s) 368
BstAFI CTTAAG 1 cut(s) 2429
BstBAI YACGTR 2 cut(s) 1041, 2179
BstBI TTCGAA 3 cut(s) 47, 224, 1966
BstC8I GCNNGC 4 cut(s) 704, 1118, 1151, 2066
BstDSI CCRYGG 1 cut(s) 747
BstENI CCTNNNNNAGG 1 cut(s) 1138
BstF5I GGATG 7 cut(s) 136, 742, 949, 1213, 1883, 1895, 2343
BstKTI GATC 9 cut(s) 79, 220, 309, 391, 451, 1093, 1679, 1756, 1795
BstMAI GTCTC 2 cut(s) 2184, 2252
BstMBI GATC 9 cut(s) 76, 217, 306, 388, 448, 1090, 1676, 1753, 1792
BstMCI CGRYCG 1 cut(s) 2122
BstMWI GCNNNNNNNGC 4 cut(s) 283, 433, 2024, 2101
BstNSI RCATGY 2 cut(s) 415, 1820
BstSFI CTRYAG 4 cut(s) 576, 1914, 1985, 1996
BstSNI TACGTA 1 cut(s) 2179
BstV1I GCAGC 5 cut(s) 46, 445, 862, 1822, 1982
BstV2I GAAGAC 5 cut(s) 904, 1355, 1376, 1402, 1643
BstX2I RGATCY 4 cut(s) 76, 1090, 1676, 1792
BstYI RGATCY 4 cut(s) 76, 1090, 1676, 1792
Bsu36I CCTNAGG 1 cut(s) 731
BsuI GTATCC 1 cut(s) 1699
BsuRI GGCC 1 cut(s) 427
BtgI CCRYGG 1 cut(s) 747
BtrI CACGTC 1 cut(s) 2112
BtsCI GGATG 7 cut(s) 136, 742, 949, 1213, 1883, 1895, 2343
BtsI GCAGTG 4 cut(s) 150, 169, 252, 341
BtsIMutI CAGTG 6 cut(s) 150, 169, 252, 341, 863, 1162
Cac8I GCNNGC 4 cut(s) 704, 1118, 1151, 2066
CaiI CAGNNNCTG 1 cut(s) 2018
Cfr13I GGNCC 2 cut(s) 735, 1315
CseI GACGC 2 cut(s) 357, 1841
Csp6I GTAC 6 cut(s) 95, 1556, 2001, 2107, 2176, 2180
CviAII CATG 8 cut(s) 92, 158, 412, 437, 1319, 1817, 1892, 2096
CviQI GTAC 6 cut(s) 95, 1556, 2001, 2107, 2176, 2180
DpnI GATC 9 cut(s) 78, 219, 308, 390, 450, 1092, 1678, 1755, 1794
DpnII GATC 9 cut(s) 76, 217, 306, 388, 448, 1090, 1676, 1753, 1792
DrdI GACNNNNNNGTC 1 cut(s) 1408
DseDI GACNNNNNNGTC 1 cut(s) 1408
Eam1104I CTCTTC 4 cut(s) 525, 1191, 2054, 2321
EarI CTCTTC 4 cut(s) 525, 1191, 2054, 2321
Eco105I TACGTA 1 cut(s) 2179
Eco130I CCWWGG 2 cut(s) 255, 492
Eco147I AGGCCT 1 cut(s) 427
Eco24I GRGCYC 1 cut(s) 180
Eco47I GGWCC 2 cut(s) 735, 1315
Eco57I CTGAAG 2 cut(s) 1020, 2025
Eco81I CCTNAGG 1 cut(s) 731
Eco88I CYCGRG 4 cut(s) 179, 236, 795, 1094
EcoNI CCTNNNNNAGG 1 cut(s) 1138
EcoO109I RGGNCCY 1 cut(s) 735
EcoRI GAATTC 1 cut(s) 1513
EcoT14I CCWWGG 2 cut(s) 255, 492
EcoT22I ATGCAT 1 cut(s) 1897
EcoT38I GRGCYC 1 cut(s) 180
ErhI CCWWGG 2 cut(s) 255, 492
FaeI CATG 8 cut(s) 95, 161, 415, 440, 1322, 1820, 1895, 2099
FaqI GGGAC 2 cut(s) 1328, 1396
FatI CATG 8 cut(s) 91, 157, 411, 436, 1318, 1816, 1891, 2095
FbaI TGATCA 1 cut(s) 448
Fnu4HI GCNGC 5 cut(s) 35, 434, 851, 1836, 1996
FokI GGATG 7 cut(s) 143, 729, 936, 1220, 1870, 1882, 2350
FriOI GRGCYC 1 cut(s) 180
Fsp4HI GCNGC 5 cut(s) 35, 434, 851, 1836, 1996
FspBI CTAG 4 cut(s) 1353, 1500, 1623, 1800
GluI GCNGC 5 cut(s) 35, 434, 851, 1836, 1996
GsuI CTGGAG 2 cut(s) 263, 1028
HaeIII GGCC 1 cut(s) 427
HapII CCGG 2 cut(s) 372, 2363
HgaI GACGC 2 cut(s) 357, 1841
Hin1I GRCGYC 1 cut(s) 368
Hin1II CATG 8 cut(s) 95, 161, 415, 440, 1322, 1820, 1895, 2099
HincII GTYRAC 1 cut(s) 1339
HindII GTYRAC 1 cut(s) 1339
HindIII AAGCTT 5 cut(s) 584, 1451, 1782, 2066, 2249
HpaII CCGG 2 cut(s) 372, 2363
HphI GGTGA 5 cut(s) 131, 458, 1532, 1597, 2366
Hpy166II GTNNAC 4 cut(s) 1339, 1662, 2109, 2203
Hpy8I GTNNAC 4 cut(s) 1339, 1662, 2109, 2203
HpyCH4III ACNGT 7 cut(s) 319, 331, 751, 839, 1104, 1659, 2344
HpyCH4IV ACGT 4 cut(s) 1040, 1572, 2111, 2178
HpyF10VI GCNNNNNNNGC 4 cut(s) 283, 433, 2024, 2101
HpySE526I ACGT 4 cut(s) 1040, 1572, 2111, 2178
Hsp92I GRCGYC 1 cut(s) 368
Hsp92II CATG 8 cut(s) 95, 161, 415, 440, 1322, 1820, 1895, 2099
Kpn2I TCCGGA 1 cut(s) 371
Ksp22I TGATCA 1 cut(s) 448
Kzo9I GATC 9 cut(s) 76, 217, 306, 388, 448, 1090, 1676, 1753, 1792
LguI GCTCTTC 2 cut(s) 525, 2054
LmnI GCTCC 5 cut(s) 282, 294, 887, 2032, 2172
Lsp1109I GCAGC 5 cut(s) 46, 445, 862, 1822, 1982
LweI GCATC 9 cut(s) 478, 595, 716, 799, 862, 1708, 1904, 1999, 2241
MaeI CTAG 4 cut(s) 1353, 1500, 1623, 1800
MaeII ACGT 4 cut(s) 1040, 1572, 2111, 2178
MaeIII GTNAC 2 cut(s) 689, 1098
MalI GATC 9 cut(s) 78, 219, 308, 390, 450, 1092, 1678, 1755, 1794
MboI GATC 9 cut(s) 76, 217, 306, 388, 448, 1090, 1676, 1753, 1792
MflI RGATCY 4 cut(s) 76, 1090, 1676, 1792
MhlI GDGCHC 2 cut(s) 180, 2029
MlyI GAGTC 5 cut(s) 466, 647, 761, 1040, 1418
Mph1103I ATGCAT 1 cut(s) 1897
MroI TCCGGA 1 cut(s) 371
MroXI GAANNNNTTC 1 cut(s) 2312
MseI TTAA 6 cut(s) 525, 1323, 1593, 1823, 1976, 2430
MslI CAYNNNNRTG 4 cut(s) 162, 2135, 2198, 2237
MspCI CTTAAG 1 cut(s) 2429
MspI CCGG 2 cut(s) 372, 2363
Mva1269I GAATGC 1 cut(s) 364
MwoI GCNNNNNNNGC 4 cut(s) 283, 433, 2024, 2101
NdeII GATC 9 cut(s) 76, 217, 306, 388, 448, 1090, 1676, 1753, 1792
NlaIII CATG 8 cut(s) 95, 161, 415, 440, 1322, 1820, 1895, 2099
NlaIV GGNNCC 4 cut(s) 883, 1092, 1316, 2168
NmeAIII GCCGAG 1 cut(s) 312
NmuCI GTSAC 1 cut(s) 689
NsiI ATGCAT 1 cut(s) 1897
NspI RCATGY 2 cut(s) 415, 1820
NspV TTCGAA 3 cut(s) 47, 224, 1966
OliI CACNNNNGTG 2 cut(s) 2135, 2198
PaeR7I CTCGAG 1 cut(s) 179
PceI AGGCCT 1 cut(s) 427
PciSI GCTCTTC 2 cut(s) 525, 2054
PctI GAATGC 1 cut(s) 364
PdmI GAANNNNTTC 1 cut(s) 2312
PfeI GAWTC 9 cut(s) 5, 271, 671, 779, 789, 1048, 1162, 2035, 2424
Pfl23II CGTACG 1 cut(s) 2175
PflMI CCANNNNNTGG 1 cut(s) 108
PkrI GCNGC 5 cut(s) 36, 435, 852, 1837, 1997
PleI GAGTC 5 cut(s) 465, 646, 761, 1039, 1417
PpsI GAGTC 5 cut(s) 465, 646, 761, 1039, 1417
Ppu21I YACGTR 2 cut(s) 1041, 2179
PpuMI RGGWCCY 1 cut(s) 735
PsiI TTATAA 2 cut(s) 512, 2052
Psp5II RGGWCCY 1 cut(s) 735
PspLI CGTACG 1 cut(s) 2175
PspN4I GGNNCC 4 cut(s) 883, 1092, 1316, 2168
PspPI GGNCC 2 cut(s) 735, 1315
PspPPI RGGWCCY 1 cut(s) 735
PstI CTGCAG 1 cut(s) 2000
PstNI CAGNNNCTG 1 cut(s) 2018
PsuI RGATCY 4 cut(s) 76, 1090, 1676, 1792
RsaI GTAC 6 cut(s) 96, 1557, 2002, 2108, 2177, 2181
RsaNI GTAC 6 cut(s) 95, 1556, 2001, 2107, 2176, 2180
RseI CAYNNNNRTG 4 cut(s) 162, 2135, 2198, 2237
SapI GCTCTTC 2 cut(s) 525, 2054
SaqAI TTAA 6 cut(s) 525, 1323, 1593, 1823, 1976, 2430
SatI GCNGC 5 cut(s) 35, 434, 851, 1836, 1996
Sau3AI GATC 9 cut(s) 76, 217, 306, 388, 448, 1090, 1676, 1753, 1792
Sau96I GGNCC 2 cut(s) 735, 1315
ScaI AGTACT 1 cut(s) 2002
SchI GAGTC 5 cut(s) 466, 647, 761, 1040, 1418
SduI GDGCHC 2 cut(s) 180, 2029
SfaNI GCATC 9 cut(s) 478, 595, 716, 799, 862, 1708, 1904, 1999, 2241
SfcI CTRYAG 4 cut(s) 576, 1914, 1985, 1996
Sfr274I CTCGAG 1 cut(s) 179
SfuI TTCGAA 3 cut(s) 47, 224, 1966
SinI GGWCC 2 cut(s) 735, 1315
SlaI CTCGAG 1 cut(s) 179
SmiMI CAYNNNNRTG 4 cut(s) 162, 2135, 2198, 2237
SmlI CTYRAG 3 cut(s) 179, 2252, 2429
SmoI CTYRAG 3 cut(s) 179, 2252, 2429
SnaBI TACGTA 1 cut(s) 2179
SpeI ACTAGT 1 cut(s) 1352
SseBI AGGCCT 1 cut(s) 427
SsiI CCGC 4 cut(s) 608, 1778, 1971, 2104
SspMI CTAG 4 cut(s) 1353, 1500, 1623, 1800
StuI AGGCCT 1 cut(s) 427
StyI CCWWGG 2 cut(s) 255, 492
TaaI ACNGT 7 cut(s) 319, 331, 751, 839, 1104, 1659, 2344
TaiI ACGT 4 cut(s) 1043, 1575, 2114, 2181
TaqI TCGA 7 cut(s) 47, 180, 224, 503, 1439, 1966, 2148
TaqII GACCGA 1 cut(s) 2108
TatI WGTACW 2 cut(s) 94, 2000
TfiI GAWTC 9 cut(s) 5, 271, 671, 779, 789, 1048, 1162, 2035, 2424
Tru1I TTAA 6 cut(s) 525, 1323, 1593, 1823, 1976, 2430
Tru9I TTAA 6 cut(s) 525, 1323, 1593, 1823, 1976, 2430
TscAI CASTG 6 cut(s) 150, 169, 252, 341, 863, 1162
TseFI GTSAC 1 cut(s) 689
TseI GCWGC 5 cut(s) 34, 433, 850, 1835, 1995
Tsp45I GTSAC 1 cut(s) 689
TspDTI ATGAA 8 cut(s) 80, 645, 1040, 1224, 1305, 1830, 1841, 2255
TspGWI ACGGA 1 cut(s) 1356
TspRI CASTG 6 cut(s) 150, 169, 252, 341, 863, 1162
Van91I CCANNNNNTGG 1 cut(s) 108
Vha464I CTTAAG 1 cut(s) 2429
VpaK11BI GGWCC 2 cut(s) 735, 1315
XagI CCTNNNNNAGG 1 cut(s) 1138
XapI RAATTY 3 cut(s) 1325, 1513, 1729
XceI RCATGY 2 cut(s) 415, 1820
XhoI CTCGAG 1 cut(s) 179
XmnI GAANNNNTTC 1 cut(s) 2312
XspI CTAG 4 cut(s) 1353, 1500, 1623, 1800
ZrmI AGTACT 1 cut(s) 2002
Zsp2I ATGCAT 1 cut(s) 1897
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.