Rw4G010540

metal ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000343
Physical Location & Seq
Forward (+)
24439079 .. 24444381
5303 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G010540.1

Sequence Viewer

Length: 2886 bp
ATGGCATCGGCAGAGTGTCAACCATATTCTGTTTCGGAGTACCATTTCGTAGATGACAAGGATGAATGTGTCTCATTTCATGTATTGCCTGTTCAATGGAGTGATGGTGAGAGACTGGATGGTGAGAAGGTAAAGATTTTTCTGCATGGAACCGCTGATAATGGGGGGAATTTATGTAAGCATGTTGTTGCATGGAAGTTTGACATTTCGAGTGAAGAGCCCGAGATATTGGTTCTCCTCTCCGAGGAAAATAGTTGGATCAAGCTTGGGAAGCCGAGGAAGAGCTTTGAGGACACTGTTAGGTCGATCTTGATAACGGTGCACTGCCTTCACTATGTGAAGAGAAATCCTGAAAAATCCAGAGAATGTGTGTGGAATCACCTATCTGAAGTTTTCAGGTTGTATGTGGTCAGGCCTTCTCAAAGTGATCTGGTGGATCACATGCCTCTAATCGGTGATTGTGTTAACAGATATCGTGCCCTAGCGGATTCCAAGTTTTTGGCCAAACTTTTTGAAGAGAAGAGGAAACTCTCTGATGAGGATACTGAAGCTAGAAAGAAGCTCAGAATTATGATCGATGATGTAAAAGATGATACGATTCATGCAGAAGATGAGGATATCGATAACCTGTTTGGTGAGGTCTGTGCTTTATGTGACGATGGTGGCAAGATCATTTGTTGTGAAGGGGGGTGCTTAAGGTCTTTTCATGCAACTGAGGAGGCGGGTGCTGGATCTGATTGCAAATCTCTTGGCTTTATGCAGGATGATGTTGTTGGGATGCAAAATTTCTACTGCAAGAACTGTGAATATAAACAGCACCAGTGCTTTGTTTGTGGGAAGTTAGGTTCCTCTGATAAGTCCTCAGGTGCTGCTGAGGTGGTTTCCTGTGTATCCCCAAACTGTGGTCGATTTTACCATCCACATTGTGTTGCAATATTACTTTATCAAGATAATGGAGTTCCTGCTGAAGAACTTGAGAGAAAGATTGCTGGTGGGGAATCTTTTACTTGTCCACTTCATAAGTGCTGCATTTGCAAACAAGGAGAAAATAAGAAGGATAGTGAGTTACAATTTGCTGTGTGCATGCGTTGTCCAAAATCATACCACAGGAAATGCCTGCCAAGTGAGATTGCTTTTGAAAATCAAGGAGAAATGCTAGAAGGGAGATCAATAATAAGAGCTTGGGATGGTCTATTACCTAACCGTATACTCATATATTGCACAAAACATGATATAGATATTGAATTTGGAACTGTAAGAAGGGACCACGTAAAATTCCCTGATGTCAAACAGAATATGAGCACTCTCAAAAAGAAGAAGAAGACTATTTTAGAAGAGAAGTGGAAGCCAATATCAGAATTCCTTGAAGACGGAGACAAAGTTGGGACTAAGAGAAATATTTCCTTGGAAAAATCATGTAGGGATAAAAATGCTCCTACAGCACTTAAGACACAAAAGCCATATGTTCAAGAGAAGAAGACTAGGAAACTTATGTCAGAGCAGAAACCTGTGTCTAAGAAGAGCAATTCTTCTTTGGAAGAATCAGTTGGGGAAGGAAGTGATCCTGTACTATCCACACTAGTCAAGCAGAAGCTTTATTCAGTTAAAGAGAAGAAGGCTACTTTTGTAAAAGAGAAAACTTTTGTTGAAGAGAAAGAAAGCCAACTGACATCAACGGTCTCTGTAGACAGAGAAAAAGCTGTGTTGAAGAGGAGAACTCCTTCCTCTGAAGGACTATATAGTGAGAAAAGTGCTCCTACAATATCCAAGAAAAAACAGAAGCCATCTTTTGTTGAAGAGAAGAACAATGACCTTGTAAAAAAGAAAACTAATGTTGAAGAGAAGAGGAAACAGACATCAGAGTCCCTTGTTGAGAGAGAGACAGCTGTGTCCAAAAAGAAAAATCCTTCCTCGAAAGAATCCTATAGGGAAAGAAATGCTTCTACAACTCCCAAACAGAAGCAGAAGCGATCAGAGCTCACTGTAGATGGAGGTGAGGCTGGGTCTAAGAAGGGAAGTCCTTTGGAAGAATCATTAAGTGCAATTGCTTCAACTGTACCCAAACTGAAGCAGAAGTCGACTTCTGCCATGAAACTGGGTAGTAACAAGAATAGGGAAAAGACGTTTTCTGGTATAGATATTTCAAGGATGGTGAAACGAAATAATTCACTGAAAAATGAACATCAGGCTTCCATGTCTGAGAAAATCAAGACCTCACTGGGTGAAGAGCCATTTTGTTCAATGGAATCTGAGCAGGTGAAGCTCGGGAACCTGGATACTCCTGATGCTAATACAGTTAGTGAGATACACAGGGAAATGCCACCACATACTGAATGTGGTGACGATGGAAATCAGCATCCTAACCCCAGCAAGTCTCGTATGCACTGTGCTCATGCTAACAGTCGAGTCCAATTCAAAATTCCTGATGATGCAGGCAGAAGGTGCACCACGAGTAATGATGAGCCTCGTTCGAGTGTTACCAATCCTAGGTCTGGCAATGCGAAAGGGTGCAAGAGAGAGAACTCGGGAAAACCATATTCAAATGGGACGAAACAGAAACAGAAACTGAGAACAGAATTTGACAGAGTCCCACCGGCTTGCATTAAAGGGAAGCAAGATCCTATTTTTTCAAGAGGCAATGATCTAGCGGGCCAAAATTCAGTGTATGACCATATTAGGACGTTCTCATCTACTTTTTACAGCCATATAGGTCCTGTTGCTGACTCGTTATCTTGTATCAACAGATTAGCTATGCCATCAGATGCAACTAAATTGGTTGGTCCAAGAGCACCCCAACCTGATTACCTGGATAGGTGTATGGGTTTCGCTCCTGGTCCGCACCTGAACTATGCAGTACAGAATTCAGCTGGTTGGATCGATGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006325 GO:0006355 GO:0006950 GO:0006952 GO:0006996 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0009605 GO:0009607 GO:0009620 GO:0009791 GO:0009889 GO:0009893 GO:0009909 GO:0009911 GO:0009987 GO:0010228 GO:0010468 GO:0010556 GO:0010604 GO:0010638 GO:0016043 GO:0019219 GO:0019222 GO:0022414 GO:0023052 GO:0030154 GO:0031056 GO:0031058 GO:0031060 GO:0031062 GO:0031323 GO:0031325 GO:0031326 GO:0031399 GO:0031401 GO:0031440 GO:0031935 GO:0031937 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033043 GO:0033044 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0043954 GO:0044030 GO:0044424 GO:0044464 GO:0048366 GO:0048367 GO:0048468 GO:0048518 GO:0048522 GO:0048580 GO:0048582 GO:0048608 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0048869 GO:0050684 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051052 GO:0051094 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051276 GO:0051570 GO:0051574 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060968 GO:0061085 GO:0061087 GO:0061458 GO:0065007 GO:0070827 GO:0070828 GO:0070829 GO:0071840 GO:0080090 GO:0090436 GO:0097159 GO:0098542 GO:0099402 GO:0140110 GO:1900109 GO:1900111 GO:1900363 GO:1901363 GO:1902275 GO:1903311 GO:1903506 GO:1905269 GO:2000024 GO:2000026 GO:2000112 GO:2000241 GO:2000243 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNMT1-RFD

Protein Analysis

961

Amino Acids

107.84

Weight (kDa)

8.57

Isoelectric Point (pI)

50.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNMT1-RFD PF12047 11 - 133 7.5e-10 Cytosine specific DNA methyltransferase replication foci domain
PHD_NSD PF22908 271 - 340 3.3e-19 Histone-lysine N-methyltransferase NSD-like, PHD zinc finger
PHDvar_NSD PF23004 342 - 378 8.1e-06 Histone-lysine N-methyltransferase NSD-like, variant PHD zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000343)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48090 AT5G48090 AT5G48090 AT5G55390 AT5G55390 AT5G55390
fragaria_vesca FvH4_3g23690 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160
malus_domestica MD03G1191000.v1.1 MD11G1207800.v1.1 MD11G1208000.v1.1
prunus_persica Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227600_v2.0.a1 Prupe.4G227700_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1
pyrus_communis pycom01g03400 pycom03g14420 pycom11g18100 pycom11g18140 pycom11g18150 pycom11g18160
rosa_chinensis RchiOBHm_Chr2g0145981 RchiOBHm_Chr4g0405241 RchiOBHm_Chr4g0405271 RchiOBHm_Chr5g0016201 RchiOBHm_Chr5g0041831 RchiOBHm_Chr5g0041871 RchiOBHm_Chr5g0041881 RchiOBHm_Chr5g0059241
rosa_laevigata RLG00000002117 RLG00000008861 RLG00000008862 RLG00000011000 RLG00000033216 RLG00000034097 RLG00000034101
rosa_multiflora Rmu_sc0000161.1_g000001 Rmu_sc0000861.1_g000040 Rmu_sc0007522.1_g000003 Rmu_sc0009443.1_g000015 Rmu_sc0022129.1_g000001 Rmu_sc0042315.1_g000001
rosa_roxburghii Rroxscaffold_1G00038660 Rroxscaffold_1G00038690 Rroxscaffold_5G00349560 Rroxscaffold_5G00349610 Rroxscaffold_6G00388370 Rroxscaffold_7G00188740
rosa_rugosa Rorug03G0199700 Rorug04G0055400 Rorug04G0055500 Rorug04G0055900 Rorug04G0056000 Rorug04G0056100 Rorug04G0056100 Rorug04G0056200 Rorug05G0195600 Rorug05G0195700 Rorug05G0195800 Rorug05G0195900 Rorug05G0196800 Rorug05G0385000
rosa_samantha Rh4AG129900 Rh4AG130100 Rh4BG124200 Rh4BG124400 Rh4BG124500 Rh4CG137200 Rh4CG137300 Rh4DG123500 Rh4DG123700 Rh5AG280800 Rh5AG281400 Rh5BG286700 Rh5BG287000 Rh5BG397800 Rh5CG318100 Rh5CG318600 Rh5CG421600 Rh5DG132300 Rh5DG132400 Rh5DG295400 Rh5DG295700 Rh7BG475300
rosa_wichuraiana Rw0G001330 Rw0G005730 Rw4G008670 Rw4G010510 Rw4G010540 Rw5G026450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2245
AasI GACNNNNNNGTC 2 cut(s) 1861, 1888
Acc36I ACCTGC 1 cut(s) 2245
AccB7I CCANNNNNTGG 1 cut(s) 902
AccI GTMKAC 3 cut(s) 1207, 1686, 2078
AciI CCGC 5 cut(s) 153, 485, 722, 2648, 2837
AclWI GGATC 6 cut(s) 266, 444, 739, 1556, 2612, 2882
AcoI YGGCCR 1 cut(s) 501
AcsI RAATTY 9 cut(s) 169, 784, 1244, 1274, 1358, 2418, 2576, 2656, 2860
AcuI CTGAAG 5 cut(s) 408, 567, 987, 1749, 2087
AdeI CACNNNGTG 3 cut(s) 337, 926, 2222
AfaI GTAC 4 cut(s) 41, 1569, 2058, 2856
AfiI CCNNNNNNNGG 5 cut(s) 244, 452, 902, 2487, 2492
AflII CTTAAG 2 cut(s) 694, 1445
AhlI ACTAGT 1 cut(s) 1579
AjnI CCWGG 3 cut(s) 2271, 2805, 2830
AjuI GAANNNNNNNTTGG 4 cut(s) 1530, 1562, 2403, 2435
AloI GAACNNNNNNTCC 2 cut(s) 829, 861
Alw21I GWGCWC 7 cut(s) 324, 1304, 1756, 1980, 2392, 2447, 2791
Alw26I GTCTC 6 cut(s) 76, 106, 1368, 1684, 1874, 2379
Alw44I GTGCAC 2 cut(s) 320, 2443
AlwI GGATC 6 cut(s) 266, 444, 739, 1556, 2612, 2882
AlwNI CAGNNNCTG 2 cut(s) 869, 2566
Ama87I CYCGRG 3 cut(s) 221, 2264, 2524
AoxI GGCC 3 cut(s) 413, 501, 2650
ApaLI GTGCAC 2 cut(s) 320, 2443
ApeKI GCWGC 2 cut(s) 869, 1026
ApoI RAATTY 9 cut(s) 169, 784, 1244, 1274, 1358, 2418, 2576, 2656, 2860
Asp700I GAANNNNTTC 4 cut(s) 1399, 1720, 1939, 2164
AspA2I CCTAGG 1 cut(s) 2486
AspS9I GGNCC 5 cut(s) 1264, 2650, 2711, 2780, 2834
AvaI CYCGRG 3 cut(s) 221, 2264, 2524
AvaII GGWCC 4 cut(s) 1264, 2711, 2780, 2834
AvrII CCTAGG 1 cut(s) 2486
AxyI CCTNAGG 1 cut(s) 862
BaeGI GKGCMC 3 cut(s) 324, 481, 2447
BalI TGGCCA 1 cut(s) 503
BanII GRGCYC 2 cut(s) 222, 1980
BauI CACGAG 1 cut(s) 2449
BbsI GAAGAC 3 cut(s) 1328, 1374, 1484
Bbv12I GWGCWC 7 cut(s) 324, 1304, 1756, 1980, 2392, 2447, 2791
BbvCI CCTCAGC 1 cut(s) 873
BbvI GCAGC 2 cut(s) 856, 1013
BciT130I CCWGG 3 cut(s) 2273, 2807, 2832
BciVI GTATCC 3 cut(s) 535, 901, 2269
BcoDI GTCTC 6 cut(s) 76, 106, 1368, 1684, 1874, 2379
BcuI ACTAGT 1 cut(s) 1579
BfaI CTAG 7 cut(s) 482, 552, 1157, 1482, 1580, 2487, 2645
BfmI CTRYAG 4 cut(s) 1437, 1683, 1924, 1983
BfrI CTTAAG 2 cut(s) 694, 1445
BfuAI ACCTGC 1 cut(s) 2245
BfuI GTATCC 3 cut(s) 535, 901, 2269
BisI GCNGC 2 cut(s) 870, 1027
BlnI CCTAGG 1 cut(s) 2486
BlsI GCNGC 2 cut(s) 871, 1028
Bme1390I CCNGG 3 cut(s) 2273, 2807, 2832
Bme18I GGWCC 4 cut(s) 1264, 2711, 2780, 2834
BmeT110I CYCGRG 3 cut(s) 221, 2264, 2524
BmgT120I GGNCC 5 cut(s) 1264, 2650, 2711, 2780, 2834
BmiI GGNNCC 4 cut(s) 151, 847, 1265, 2270
BmrFI CCNGG 3 cut(s) 2273, 2807, 2832
BmrI ACTGGG 2 cut(s) 2105, 2228
BmsI GCATC 6 cut(s) 14, 768, 2275, 2365, 2419, 2752
BmuI ACTGGG 2 cut(s) 2105, 2228
BpiI GAAGAC 3 cut(s) 1328, 1374, 1484
BplI GAGNNNNNCTC 2 cut(s) 1702, 1734
Bpu10I CCTNAGC 1 cut(s) 873
BpuEI CTTGAG 1 cut(s) 995
Bsa29I ATCGAT 3 cut(s) 576, 621, 2877
BsaAI YACGTR 1 cut(s) 1270
BsaBI GATNNNNATC 1 cut(s) 2349
BsaI GGTCTC 1 cut(s) 1684
BsaJI CCNNGG 4 cut(s) 243, 275, 1404, 2486
BsaXI ACNNNNNCTCC 4 cut(s) 91, 121, 1365, 1395
Bsc4I CCNNNNNNNGG 5 cut(s) 244, 452, 902, 2487, 2492
Bse118I RCCGGY 1 cut(s) 2593
Bse1I ACTGG 4 cut(s) 120, 820, 2100, 2223
Bse21I CCTNAGG 1 cut(s) 862
Bse3DI GCAATG 2 cut(s) 2503, 2644
Bse8I GATNNNNATC 1 cut(s) 2349
BseBI CCWGG 3 cut(s) 2273, 2807, 2832
BseCI ATCGAT 3 cut(s) 576, 621, 2877
BseDI CCNNGG 4 cut(s) 243, 275, 1404, 2486
BseGI GGATG 8 cut(s) 67, 124, 769, 783, 916, 1192, 2154, 2356
BseJI GATNNNNATC 1 cut(s) 2349
BseLI CCNNNNNNNGG 5 cut(s) 244, 452, 902, 2487, 2492
BseMI GCAATG 2 cut(s) 2503, 2644
BseMII CTCAG 7 cut(s) 577, 705, 864, 876, 2190, 2241, 2558
BseNI ACTGG 4 cut(s) 120, 820, 2100, 2223
BseRI GAGGAG 3 cut(s) 227, 731, 1726
BseSI GKGCMC 3 cut(s) 324, 481, 2447
BseXI GCAGC 2 cut(s) 856, 1013
BseYI CCCAGC 2 cut(s) 2000, 2366
BshFI GGCC 3 cut(s) 415, 503, 2652
BshVI ATCGAT 3 cut(s) 576, 621, 2877
BsiHKAI GWGCWC 7 cut(s) 324, 1304, 1756, 1980, 2392, 2447, 2791
BsiHKCI CYCGRG 3 cut(s) 221, 2264, 2524
BsiSI CCGG 1 cut(s) 2594
BslFI GGGAC 5 cut(s) 1277, 1399, 1849, 2560, 2573
BslI CCNNNNNNNGG 5 cut(s) 244, 452, 902, 2487, 2492
BsmAI GTCTC 6 cut(s) 76, 106, 1368, 1684, 1874, 2379
BsmFI GGGAC 5 cut(s) 1277, 1399, 1849, 2560, 2573
BsnI GGCC 3 cut(s) 415, 503, 2652
Bso31I GGTCTC 1 cut(s) 1684
BsoBI CYCGRG 3 cut(s) 221, 2264, 2524
Bsp1286I GDGCHC 9 cut(s) 222, 324, 481, 1304, 1756, 1980, 2392, 2447, 2791
BspACI CCGC 5 cut(s) 153, 485, 722, 2648, 2837
BspANI GGCC 3 cut(s) 415, 503, 2652
BspCNI CTCAG 7 cut(s) 576, 706, 865, 875, 2191, 2242, 2559
BspDI ATCGAT 3 cut(s) 576, 621, 2877
BspLI GGNNCC 4 cut(s) 151, 847, 1265, 2270
BspMI ACCTGC 1 cut(s) 2245
BspPI GGATC 6 cut(s) 266, 444, 739, 1556, 2612, 2882
BspQI GCTCTTC 4 cut(s) 210, 275, 1514, 2220
BspTI CTTAAG 2 cut(s) 694, 1445
BspTNI GGTCTC 1 cut(s) 1684
BsrDI GCAATG 2 cut(s) 2503, 2644
BsrFI RCCGGY 1 cut(s) 2593
BsrI ACTGG 4 cut(s) 120, 820, 2100, 2223
BssAI RCCGGY 1 cut(s) 2593
BssECI CCNNGG 4 cut(s) 243, 275, 1404, 2486
BssNAI GTATAC 1 cut(s) 1208
BssSI CACGAG 1 cut(s) 2449
BssT1I CCWWGG 2 cut(s) 1404, 2486
Bst1107I GTATAC 1 cut(s) 1208
Bst2BI CACGAG 1 cut(s) 2449
Bst2UI CCWGG 3 cut(s) 2273, 2807, 2832
BstAFI CTTAAG 2 cut(s) 694, 1445
BstAPI GCANNNNNTGC 1 cut(s) 2442
BstBAI YACGTR 1 cut(s) 1270
BstC8I GCNNGC 5 cut(s) 1085, 1118, 2434, 2599, 2650
BstENI CCTNNNNNAGG 1 cut(s) 242
BstF5I GGATG 8 cut(s) 67, 124, 769, 783, 916, 1192, 2154, 2356
BstMAI GTCTC 6 cut(s) 76, 106, 1368, 1684, 1874, 2379
BstMWI GCNNNNNNNGC 6 cut(s) 271, 1032, 1439, 1975, 2260, 2442
BstNI CCWGG 3 cut(s) 2273, 2807, 2832
BstNSI RCATGY 3 cut(s) 185, 445, 1087
BstSCI CCNGG 3 cut(s) 2271, 2805, 2830
BstSFI CTRYAG 4 cut(s) 1437, 1683, 1924, 1983
BstSLI GKGCMC 3 cut(s) 324, 481, 2447
BstV1I GCAGC 2 cut(s) 856, 1013
BstV2I GAAGAC 3 cut(s) 1328, 1374, 1484
BstX2I RGATCY 2 cut(s) 731, 2617
BstXI CCANNNNNNTGG 2 cut(s) 499, 2095
BstYI RGATCY 2 cut(s) 731, 2617
BstZ17I GTATAC 1 cut(s) 1208
Bsu15I ATCGAT 3 cut(s) 576, 621, 2877
Bsu36I CCTNAGG 1 cut(s) 862
BsuI GTATCC 3 cut(s) 535, 901, 2269
BsuRI GGCC 3 cut(s) 415, 503, 2652
BsuTUI ATCGAT 3 cut(s) 576, 621, 2877
BtsCI GGATG 8 cut(s) 67, 124, 769, 783, 916, 1192, 2154, 2356
BtsI GCAGTG 1 cut(s) 322
BtsIMutI CAGTG 8 cut(s) 294, 322, 827, 1980, 2168, 2216, 2383, 2667
BveI ACCTGC 1 cut(s) 2245
Cac8I GCNNGC 5 cut(s) 1085, 1118, 2434, 2599, 2650
CaiI CAGNNNCTG 2 cut(s) 869, 2566
Cfr10I RCCGGY 1 cut(s) 2593
Cfr13I GGNCC 5 cut(s) 1264, 2650, 2711, 2780, 2834
ClaI ATCGAT 3 cut(s) 576, 621, 2877
Csp6I GTAC 4 cut(s) 40, 1568, 2057, 2855
CviQI GTAC 4 cut(s) 40, 1568, 2057, 2855
DraIII CACNNNGTG 3 cut(s) 337, 926, 2222
DrdI GACNNNNNNGTC 2 cut(s) 1861, 1888
DseDI GACNNNNNNGTC 2 cut(s) 1861, 1888
EaeI YGGCCR 1 cut(s) 501
Ecl136II GAGCTC 1 cut(s) 1978
Eco130I CCWWGG 2 cut(s) 1404, 2486
Eco147I AGGCCT 1 cut(s) 415
Eco24I GRGCYC 2 cut(s) 222, 1980
Eco31I GGTCTC 1 cut(s) 1684
Eco32I GATATC 2 cut(s) 473, 619
Eco47I GGWCC 4 cut(s) 1264, 2711, 2780, 2834
Eco53kI GAGCTC 1 cut(s) 1978
Eco57I CTGAAG 5 cut(s) 408, 567, 987, 1749, 2087
Eco81I CCTNAGG 1 cut(s) 862
Eco88I CYCGRG 3 cut(s) 221, 2264, 2524
EcoICRI GAGCTC 1 cut(s) 1978
EcoNI CCTNNNNNAGG 1 cut(s) 242
EcoO109I RGGNCCY 1 cut(s) 2711
EcoRI GAATTC 2 cut(s) 1358, 2860
EcoRII CCWGG 3 cut(s) 2271, 2805, 2830
EcoRV GATATC 2 cut(s) 473, 619
EcoT14I CCWWGG 2 cut(s) 1404, 2486
EcoT38I GRGCYC 2 cut(s) 222, 1980
ErhI CCWWGG 2 cut(s) 1404, 2486
FalI AAGNNNNNCTT 2 cut(s) 1924, 1956
FaqI GGGAC 5 cut(s) 1277, 1399, 1849, 2560, 2573
FauI CCCGC 2 cut(s) 715, 2641
FauNDI CATATG 1 cut(s) 1462
FblI GTMKAC 3 cut(s) 1207, 1686, 2078
Fnu4HI GCNGC 2 cut(s) 870, 1027
FokI GGATG 8 cut(s) 74, 131, 776, 790, 903, 1199, 2161, 2343
FriOI GRGCYC 2 cut(s) 222, 1980
Fsp4HI GCNGC 2 cut(s) 870, 1027
FspBI CTAG 7 cut(s) 482, 552, 1157, 1482, 1580, 2487, 2645
GluI GCNGC 2 cut(s) 870, 1027
GsaI CCCAGC 2 cut(s) 2004, 2370
HaeIII GGCC 3 cut(s) 415, 503, 2652
HapII CCGG 1 cut(s) 2594
HincII GTYRAC 3 cut(s) 20, 466, 2079
HindII GTYRAC 3 cut(s) 20, 466, 2079
HindIII AAGCTT 2 cut(s) 263, 1592
HpaI GTTAAC 1 cut(s) 466
HpaII CCGG 1 cut(s) 2594
Hpy166II GTNNAC 8 cut(s) 20, 322, 466, 1013, 1208, 1687, 2079, 2445
Hpy8I GTNNAC 8 cut(s) 20, 322, 466, 1013, 1208, 1687, 2079, 2445
HpyCH4IV ACGT 3 cut(s) 1269, 2123, 2681
HpyF10VI GCNNNNNNNGC 6 cut(s) 271, 1032, 1439, 1975, 2260, 2442
HpySE526I ACGT 3 cut(s) 1269, 2123, 2681
KspAI GTTAAC 1 cut(s) 466
LguI GCTCTTC 4 cut(s) 210, 275, 1514, 2220
LmnI GCTCC 3 cut(s) 1438, 1759, 2833
Lsp1109I GCAGC 2 cut(s) 856, 1013
LweI GCATC 6 cut(s) 14, 768, 2275, 2365, 2419, 2752
MaeI CTAG 7 cut(s) 482, 552, 1157, 1482, 1580, 2487, 2645
MaeII ACGT 3 cut(s) 1269, 2123, 2681
MaeIII GTNAC 5 cut(s) 653, 1065, 2102, 2339, 2476
MfeI CAATTG 1 cut(s) 2043
MflI RGATCY 2 cut(s) 731, 2617
MhlI GDGCHC 9 cut(s) 222, 324, 481, 1304, 1756, 1980, 2392, 2447, 2791
MlsI TGGCCA 1 cut(s) 503
MluNI TGGCCA 1 cut(s) 503
MlyI GAGTC 4 cut(s) 1871, 2415, 2595, 2717
MmeI TCCRAC 2 cut(s) 236, 2852
Mox20I TGGCCA 1 cut(s) 503
MroXI GAANNNNTTC 4 cut(s) 1399, 1720, 1939, 2164
MscI TGGCCA 1 cut(s) 503
MseI TTAA 6 cut(s) 465, 695, 1446, 1605, 2036, 2604
Msp20I TGGCCA 1 cut(s) 503
MspA1I CMGCKG 3 cut(s) 155, 1886, 2867
MspCI CTTAAG 2 cut(s) 694, 1445
MspI CCGG 1 cut(s) 2594
MspR9I CCNGG 3 cut(s) 2273, 2807, 2832
MunI CAATTG 1 cut(s) 2043
MvaI CCWGG 3 cut(s) 2273, 2807, 2832
MwoI GCNNNNNNNGC 6 cut(s) 271, 1032, 1439, 1975, 2260, 2442
NdeI CATATG 1 cut(s) 1462
NlaIV GGNNCC 4 cut(s) 151, 847, 1265, 2270
NmeAIII GCCGAG 1 cut(s) 300
NmuCI GTSAC 2 cut(s) 653, 2339
NspI RCATGY 3 cut(s) 185, 445, 1087
PaeI GCATGC 1 cut(s) 1087
PaqCI CACCTGC 1 cut(s) 2245
PceI AGGCCT 1 cut(s) 415
PciSI GCTCTTC 4 cut(s) 210, 275, 1514, 2220
PdmI GAANNNNTTC 4 cut(s) 1399, 1720, 1939, 2164
PfeI GAWTC 8 cut(s) 376, 488, 598, 998, 1541, 1919, 2030, 2246
PflFI GACNNNGTC 1 cut(s) 2585
PflMI CCANNNNNTGG 1 cut(s) 902
PkrI GCNGC 2 cut(s) 871, 1028
PleI GAGTC 4 cut(s) 1870, 2414, 2594, 2717
PpsI GAGTC 4 cut(s) 1870, 2414, 2594, 2717
Ppu21I YACGTR 1 cut(s) 1270
PpuMI RGGWCCY 1 cut(s) 2711
Psp124BI GAGCTC 1 cut(s) 1980
Psp5II RGGWCCY 1 cut(s) 2711
Psp6I CCWGG 3 cut(s) 2271, 2805, 2830
PspFI CCCAGC 2 cut(s) 2000, 2366
PspGI CCWGG 3 cut(s) 2271, 2805, 2830
PspN4I GGNNCC 4 cut(s) 151, 847, 1265, 2270
PspPI GGNCC 5 cut(s) 1264, 2650, 2711, 2780, 2834
PspPPI RGGWCCY 1 cut(s) 2711
PsrI GAACNNNNNNTAC 2 cut(s) 75, 107
PstNI CAGNNNCTG 2 cut(s) 869, 2566
PsuI RGATCY 2 cut(s) 731, 2617
PsyI GACNNNGTC 1 cut(s) 2585
PvuII CAGCTG 2 cut(s) 1886, 2867
RsaI GTAC 4 cut(s) 41, 1569, 2058, 2856
RsaNI GTAC 4 cut(s) 40, 1568, 2057, 2855
SacI GAGCTC 1 cut(s) 1980
SalI GTCGAC 1 cut(s) 2077
SapI GCTCTTC 4 cut(s) 210, 275, 1514, 2220
SaqAI TTAA 6 cut(s) 465, 695, 1446, 1605, 2036, 2604
SatI GCNGC 2 cut(s) 870, 1027
Sau96I GGNCC 5 cut(s) 1264, 2650, 2711, 2780, 2834
SchI GAGTC 4 cut(s) 1871, 2415, 2595, 2717
ScrFI CCNGG 3 cut(s) 2273, 2807, 2832
SduI GDGCHC 9 cut(s) 222, 324, 481, 1304, 1756, 1980, 2392, 2447, 2791
SfaNI GCATC 6 cut(s) 14, 768, 2275, 2365, 2419, 2752
SfcI CTRYAG 4 cut(s) 1437, 1683, 1924, 1983
SinI GGWCC 4 cut(s) 1264, 2711, 2780, 2834
SmlI CTYRAG 3 cut(s) 694, 974, 1445
SmoI CTYRAG 3 cut(s) 694, 974, 1445
SpeI ACTAGT 1 cut(s) 1579
SphI GCATGC 1 cut(s) 1087
SseBI AGGCCT 1 cut(s) 415
SsiI CCGC 5 cut(s) 153, 485, 722, 2648, 2837
SspI AATATT 2 cut(s) 936, 1399
SspMI CTAG 7 cut(s) 482, 552, 1157, 1482, 1580, 2487, 2645
SstI GAGCTC 1 cut(s) 1980
StuI AGGCCT 1 cut(s) 415
StyD4I CCNGG 3 cut(s) 2271, 2805, 2830
StyI CCWWGG 2 cut(s) 1404, 2486
TaiI ACGT 3 cut(s) 1272, 2126, 2684
TatI WGTACW 2 cut(s) 1567, 2854
TfiI GAWTC 8 cut(s) 376, 488, 598, 998, 1541, 1919, 2030, 2246
Tru1I TTAA 6 cut(s) 465, 695, 1446, 1605, 2036, 2604
Tru9I TTAA 6 cut(s) 465, 695, 1446, 1605, 2036, 2604
TscAI CASTG 8 cut(s) 301, 329, 827, 1987, 2175, 2223, 2390, 2667
TseFI GTSAC 2 cut(s) 653, 2339
TseI GCWGC 2 cut(s) 869, 1026
Tsp45I GTSAC 2 cut(s) 653, 2339
TspDTI ATGAA 7 cut(s) 68, 78, 590, 695, 1007, 2105, 2193
TspGWI ACGGA 1 cut(s) 1386
TspRI CASTG 8 cut(s) 301, 329, 827, 1987, 2175, 2223, 2390, 2667
Tth111I GACNNNGTC 1 cut(s) 2585
Van91I CCANNNNNTGG 1 cut(s) 902
Vha464I CTTAAG 2 cut(s) 694, 1445
VneI GTGCAC 2 cut(s) 320, 2443
VpaK11BI GGWCC 4 cut(s) 1264, 2711, 2780, 2834
XagI CCTNNNNNAGG 1 cut(s) 242
XapI RAATTY 9 cut(s) 169, 784, 1244, 1274, 1358, 2418, 2576, 2656, 2860
XceI RCATGY 3 cut(s) 185, 445, 1087
XmaJI CCTAGG 1 cut(s) 2486
XmiI GTMKAC 3 cut(s) 1207, 1686, 2078
XmnI GAANNNNTTC 4 cut(s) 1399, 1720, 1939, 2164
XspI CTAG 7 cut(s) 482, 552, 1157, 1482, 1580, 2487, 2645
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.