Prupe.4G227800_v2.0.a1

metal ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0000343
Physical Location & Seq
Reverse (-)
14537549 .. 14543878
6330 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G227800.3

Sequence Viewer

Length: 2247 bp
ATGGCATCCTCAGATGGCGAAGCCGAAGCTCTGCCATCTACTGTGTCAAATTACCATTTTATGGATGACAGAGACGAACCCATATCATTTCATGTTCTGCCCATTCAATGGAGTGAAGGTGAGAGGCAGGGTGGCAAGAAAGTTAAGATTTTTCTGCATGGAACTGTTGATGATGGGATTCAGAAGATATATAAGCATGTTATAGCTTGGAAGTTTGACCTTTCCAAGGGAAAGCCCGAGATTTTTGTACTCTCCAAGGAAAATAATTGGCTTAGGCTTCTGAAGCCGAGGAAGAGCTTTGAGGACACGGTCCGATCAATTTTGATAATGGTTCAGTTCCTTCATTATGTGAAGAGAAATCCTGAAACATCCTACAAATCGTTGTGTACTCACTTGTCTAAAGTTTTCAGCTTGTATGACGTCAGGCATTCTCATAATGATCTGCGGGATCACATTCTGTTGATACGTGATTCTGTTGAACGGGATGATGCCTTAGCAAGGTCCAAGTTTTTGGTGAATTTTCTTGGAGAGAAGCCCGCAAAGAGGCAACTATCTGATGAGGTCATATTCTCCAGCTTATCTCTTTCCCTTCCTTTGGGCATTCAAGGTACAAATGATCTCAGATTTGTGGATAATCATATAGAACATGATATGAATGATGGTGCAGAAAATGAGTCCAGTGACATTGATAGCCTGTTTAGCTCTGTCTGTGCTTTCTGCGATGATGGTGGTGATCTCTTGTGTTGTGAAGGGAGGTGCCTAAGGTCCTTTCATCCAACTGTAGAGTCTACTGTGGGTTCTGATTGCAAATCTCTTGGCTTTACCAAGGATGAAGTAGATGCGACGCAAAATTACTTTTGCAAGAACTGTAAATATAAACAGCACCAGTGCTTTGCTTGTGGGAAGCTAGGTCCCTCTGATAAATCTTCAGGTGCAGAGGTTGTTCCATGTTTTTCTGCGATCTGTGGTCGATTTTACCATCCACGATGTGTTGCAAAATTGCTTCATCGAGATAATGGAGTTTCTGCCGAAGAACTTGAGAAAAAGATTGCTATGGGGGGGTCTTTTACATGTCCGATTCACAAGTGCTGCGTTTGTAAACAAGGAGAGAATAAGAAGGAGAGGGATTTGCAGTTTGCTGTGTGCATGCGTTGTCCTAAATCGTACCACAGGAAATGCTTGCCAAGAAAGATTGCTTTTGCAAAGGGAGGAGGAAGTGATGGAGAACGGCTATTAATACCAAGAGCATGGGAAGGTCTATTACCTAAACGCATTCTTATATATTGCACAAAACATGAAATGAATGAAGAAGGCAGAACTATTATAAGGGACCACTTAAAATTCCCTGATGTTGAAGTGAAGAAGACTGTTACAGAAGAAAATACGAAAATTTTGACATCAGAGTCCCTCGTAGGGAGAGAGAAAGTTGTGTCTAAGAAAATGGTTTCCTTGGATGACTTATATAGGGTAACAATTCCTACTGAAAGATCTGAGCAGAAGCAGAAGCCATCTGCTGAGGAAGTGGGTGGTAGAAATGGGGATAAAGTATTTTCTGGGTTTGACAGTTTGAGAAAGGTGACAATAAATACTGCATCTAAAAAAGAACTGAAGACTTTCACCTCTGAGGAAAAGAAGACTCCAGTGGGTGAACATAAATGGAGCTCCTCTAAGAGCAATGGTGAAGGAGAATGTCAGAAGAGTCTCGGCCATGGGAATAAGCGAGGCAATGCGACATTAGGAAAGCTGCAGCATGGTGGCAAAGAAAGGCACAGAAGAGGAATTGTTGAGATTTCATCAGTGAACAAGCGTTGTGGCATTGCTAATCAGCATTCTGAGTTGAGTAAGACTGGTAATATGCAGCTTGGAATGTCTAGTGGTGGAACCCAGCTCAACATTCCTGTTGACACAGGATCTTGCTCACAGGTTTGCACGTGTAGGCAACAAGTTCCTGATCTTGCACGATGTAATTTAGTGGGTCAGGATTCTGTTTATGACCGGATTGGTTTGCGGTCTTCTACTTGTGGCCATCAAGGTCTGGCAGCTGAGTCATTTTCTGGAATGAAGCCATTATCTATGGTGCCGTATGCACCTCAATGTAATATTAACTGCTCAATGCCAACTGCTTTAGTTAACAGTTATGGTCCTGGAGAGCGTAAACCTGGCTACCTTGGCAATCCTTTGGGTTTTGCTCCTGGCCCTCACCAGAACTACTCAGTCCATAATTCAGCAGGTTGGCTGGATGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006325 GO:0006355 GO:0006950 GO:0006952 GO:0006996 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0009605 GO:0009607 GO:0009620 GO:0009791 GO:0009889 GO:0009893 GO:0009909 GO:0009911 GO:0009987 GO:0010228 GO:0010468 GO:0010556 GO:0010604 GO:0010638 GO:0016043 GO:0019219 GO:0019222 GO:0022414 GO:0023052 GO:0030154 GO:0031056 GO:0031058 GO:0031060 GO:0031062 GO:0031323 GO:0031325 GO:0031326 GO:0031399 GO:0031401 GO:0031440 GO:0031935 GO:0031937 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033043 GO:0033044 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0043954 GO:0044030 GO:0044424 GO:0044464 GO:0048366 GO:0048367 GO:0048468 GO:0048518 GO:0048522 GO:0048580 GO:0048582 GO:0048608 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0048869 GO:0050684 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051052 GO:0051094 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051276 GO:0051570 GO:0051574 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060968 GO:0061085 GO:0061087 GO:0061458 GO:0065007 GO:0070827 GO:0070828 GO:0070829 GO:0071840 GO:0080090 GO:0090436 GO:0097159 GO:0098542 GO:0099402 GO:0140110 GO:1900109 GO:1900111 GO:1900363 GO:1901363 GO:1902275 GO:1903311 GO:1903506 GO:1905269 GO:2000024 GO:2000026 GO:2000112 GO:2000241 GO:2000243 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNMT1-RFD

Protein Analysis

749

Amino Acids

83.23

Weight (kDa)

8.61

Isoelectric Point (pI)

40.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000343)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48090 AT5G48090 AT5G48090 AT5G55390 AT5G55390 AT5G55390
fragaria_vesca FvH4_3g23690 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160
malus_domestica MD03G1191000.v1.1 MD11G1207800.v1.1 MD11G1208000.v1.1
prunus_persica Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227600_v2.0.a1 Prupe.4G227700_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1
pyrus_communis pycom01g03400 pycom03g14420 pycom11g18100 pycom11g18140 pycom11g18150 pycom11g18160
rosa_chinensis RchiOBHm_Chr2g0145981 RchiOBHm_Chr4g0405241 RchiOBHm_Chr4g0405271 RchiOBHm_Chr5g0016201 RchiOBHm_Chr5g0041831 RchiOBHm_Chr5g0041871 RchiOBHm_Chr5g0041881 RchiOBHm_Chr5g0059241
rosa_laevigata RLG00000002117 RLG00000008861 RLG00000008862 RLG00000011000 RLG00000033216 RLG00000034097 RLG00000034101
rosa_multiflora Rmu_sc0000161.1_g000001 Rmu_sc0000861.1_g000040 Rmu_sc0007522.1_g000003 Rmu_sc0009443.1_g000015 Rmu_sc0022129.1_g000001 Rmu_sc0042315.1_g000001
rosa_roxburghii Rroxscaffold_1G00038660 Rroxscaffold_1G00038690 Rroxscaffold_5G00349560 Rroxscaffold_5G00349610 Rroxscaffold_6G00388370 Rroxscaffold_7G00188740
rosa_rugosa Rorug03G0199700 Rorug04G0055400 Rorug04G0055500 Rorug04G0055900 Rorug04G0056000 Rorug04G0056100 Rorug04G0056100 Rorug04G0056200 Rorug05G0195600 Rorug05G0195700 Rorug05G0195800 Rorug05G0195900 Rorug05G0196800 Rorug05G0385000
rosa_samantha Rh4AG129900 Rh4AG130100 Rh4BG124200 Rh4BG124400 Rh4BG124500 Rh4CG137200 Rh4CG137300 Rh4DG123500 Rh4DG123700 Rh5AG280800 Rh5AG281400 Rh5BG286700 Rh5BG287000 Rh5BG397800 Rh5CG318100 Rh5CG318600 Rh5CG421600 Rh5DG132300 Rh5DG132400 Rh5DG295400 Rh5DG295700 Rh7BG475300
rosa_wichuraiana Rw0G001330 Rw0G005730 Rw4G008670 Rw4G010510 Rw4G010540 Rw5G026450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1325
AasI GACNNNNNNGTC 1 cut(s) 1402
AatII GACGTC 1 cut(s) 423
Acc36I ACCTGC 1 cut(s) 2219
AccB1I GGYRCC 2 cut(s) 756, 2077
AccB7I CCANNNNNTGG 2 cut(s) 61, 108
AccI GTMKAC 1 cut(s) 788
AciI CCGC 3 cut(s) 445, 537, 2008
AclWI GGATC 2 cut(s) 456, 1918
AcoI YGGCCR 2 cut(s) 1705, 2023
AcsI RAATTY 3 cut(s) 517, 1340, 1389
AcuI CTGAAG 3 cut(s) 302, 912, 1628
AcvI CACGTG 1 cut(s) 1932
AcyI GRCGYC 1 cut(s) 420
AdeI CACNNNGTG 1 cut(s) 989
AfaI GTAC 4 cut(s) 249, 388, 610, 1166
AfiI CCNNNNNNNGG 7 cut(s) 61, 108, 226, 498, 543, 595, 1413
AflIII ACRYGT 2 cut(s) 1070, 1931
AgsI TTSAA 4 cut(s) 107, 479, 605, 1355
AjnI CCWGG 3 cut(s) 2143, 2158, 2191
Alw21I GWGCWC 1 cut(s) 1664
Alw26I GTCTC 2 cut(s) 66, 1706
AlwI GGATC 2 cut(s) 456, 1918
Ama87I CYCGRG 1 cut(s) 236
AoxI GGCC 3 cut(s) 1705, 2023, 2194
ApeKI GCWGC 5 cut(s) 1089, 1744, 1747, 1858, 2039
ApoI RAATTY 3 cut(s) 517, 1340, 1389
AseI ATTAAT 1 cut(s) 1235
Asp700I GAANNNNTTC 1 cut(s) 1613
AspS9I GGNCC 7 cut(s) 310, 501, 765, 911, 1330, 2141, 2195
AsuHPI GGTGA 8 cut(s) 131, 526, 743, 1588, 1609, 1658, 1691, 2192
AvaI CYCGRG 1 cut(s) 236
AvaII GGWCC 6 cut(s) 310, 501, 765, 911, 1330, 2141
AxyI CCTNAGG 1 cut(s) 761
BalI TGGCCA 1 cut(s) 2025
BanI GGYRCC 2 cut(s) 756, 2077
BanII GRGCYC 1 cut(s) 1664
BarI GAAGNNNNNNTAC 2 cut(s) 1245, 1277
BbrPI CACGTG 1 cut(s) 1932
BbsI GAAGAC 4 cut(s) 1370, 1616, 1640, 2004
Bbv12I GWGCWC 1 cut(s) 1664
BbvCI CCTCAGC 1 cut(s) 1515
BbvI GCAGC 5 cut(s) 1076, 1731, 1759, 1870, 2051
BccI CCATC 9 cut(s) 8, 43, 167, 653, 719, 987, 1214, 1516, 2034
BceAI ACGGC 2 cut(s) 1244, 2065
BciT130I CCWGG 3 cut(s) 2145, 2160, 2193
BcoDI GTCTC 2 cut(s) 66, 1706
BfaI CTAG 2 cut(s) 908, 1872
BfmI CTRYAG 2 cut(s) 780, 1745
BfuAI ACCTGC 1 cut(s) 2219
BglII AGATCT 1 cut(s) 1487
BisI GCNGC 5 cut(s) 1090, 1745, 1748, 1859, 2040
BlsI GCNGC 5 cut(s) 1091, 1746, 1749, 1860, 2041
Bme1390I CCNGG 3 cut(s) 2145, 2160, 2193
Bme18I GGWCC 6 cut(s) 310, 501, 765, 911, 1330, 2141
BmeT110I CYCGRG 1 cut(s) 236
BmgT120I GGNCC 7 cut(s) 310, 501, 765, 911, 1330, 2141, 2195
BmiI GGNNCC 5 cut(s) 758, 913, 1331, 1882, 2079
BmrFI CCNGG 3 cut(s) 2145, 2160, 2193
BmsI GCATC 4 cut(s) 14, 478, 829, 1601
BpiI GAAGAC 4 cut(s) 1370, 1616, 1640, 2004
BpmI CTGGAG 3 cut(s) 556, 1623, 2166
Bpu10I CCTNAGC 3 cut(s) 272, 493, 1515
BpuEI CTTGAG 1 cut(s) 1058
BsaAI YACGTR 2 cut(s) 467, 1932
BsaHI GRCGYC 1 cut(s) 420
BsaJI CCNNGG 7 cut(s) 225, 255, 287, 825, 1449, 1708, 2167
BsaWI WCCGGW 1 cut(s) 1995
BsaXI ACNNNNNCTCC 2 cut(s) 103, 133
Bsc4I CCNNNNNNNGG 7 cut(s) 61, 108, 226, 498, 543, 595, 1413
Bse1I ACTGG 4 cut(s) 678, 886, 1640, 1852
Bse21I CCTNAGG 1 cut(s) 761
Bse3DI GCAATG 3 cut(s) 1681, 1732, 1815
BseBI CCWGG 3 cut(s) 2145, 2160, 2193
BseDI CCNNGG 7 cut(s) 225, 255, 287, 825, 1449, 1708, 2167
BseGI GGATG 9 cut(s) 5, 70, 368, 490, 772, 835, 979, 1459, 2245
BseLI CCNNNNNNNGG 7 cut(s) 61, 108, 226, 498, 543, 595, 1413
BseMI GCAATG 3 cut(s) 1681, 1732, 1815
BseMII CTCAG 8 cut(s) 24, 634, 1482, 1506, 1614, 1824, 2034, 2226
BseNI ACTGG 4 cut(s) 678, 886, 1640, 1852
BseRI GAGGAG 2 cut(s) 1224, 1654
BseXI GCAGC 5 cut(s) 1076, 1731, 1759, 1870, 2051
BseYI CCCAGC 1 cut(s) 1884
BsgI GTGCAG 2 cut(s) 684, 954
BshFI GGCC 3 cut(s) 1707, 2025, 2196
BshNI GGYRCC 2 cut(s) 756, 2077
BsiHKAI GWGCWC 1 cut(s) 1664
BsiHKCI CYCGRG 1 cut(s) 236
BsiSI CCGG 1 cut(s) 1996
BslFI GGGAC 3 cut(s) 897, 1343, 1390
BslI CCNNNNNNNGG 7 cut(s) 61, 108, 226, 498, 543, 595, 1413
BsmAI GTCTC 2 cut(s) 66, 1706
BsmBI CGTCTC 1 cut(s) 66
BsmFI GGGAC 3 cut(s) 897, 1343, 1390
BsmI GAATGC 4 cut(s) 427, 600, 1272, 1828
BsnI GGCC 3 cut(s) 1707, 2025, 2196
BsoBI CYCGRG 1 cut(s) 236
Bsp1286I GDGCHC 1 cut(s) 1664
Bsp143I GATC 9 cut(s) 314, 439, 448, 616, 733, 960, 1487, 1910, 1951
Bsp19I CCATGG 1 cut(s) 1708
BspACI CCGC 3 cut(s) 445, 537, 2008
BspANI GGCC 3 cut(s) 1707, 2025, 2196
BspCNI CTCAG 8 cut(s) 23, 633, 1483, 1507, 1615, 1825, 2035, 2225
BspLI GGNNCC 5 cut(s) 758, 913, 1331, 1882, 2079
BspMAI CTGCAG 1 cut(s) 1749
BspMI ACCTGC 1 cut(s) 2219
BspPI GGATC 2 cut(s) 456, 1918
BspQI GCTCTTC 1 cut(s) 287
BspT107I GGYRCC 2 cut(s) 756, 2077
BsrDI GCAATG 3 cut(s) 1681, 1732, 1815
BsrI ACTGG 4 cut(s) 678, 886, 1640, 1852
BssECI CCNNGG 7 cut(s) 225, 255, 287, 825, 1449, 1708, 2167
BssMI GATC 9 cut(s) 314, 439, 448, 616, 733, 960, 1487, 1910, 1951
BssNI GRCGYC 1 cut(s) 420
BssT1I CCWWGG 6 cut(s) 225, 255, 825, 1449, 1708, 2167
Bst2UI CCWGG 3 cut(s) 2145, 2160, 2193
Bst4CI ACNGT 9 cut(s) 43, 166, 310, 781, 793, 869, 1369, 1565, 2135
Bst6I CTCTTC 4 cut(s) 287, 347, 1691, 1768
BstACI GRCGYC 1 cut(s) 420
BstBAI YACGTR 2 cut(s) 467, 1932
BstC8I GCNNGC 3 cut(s) 537, 1148, 1181
BstDSI CCRYGG 1 cut(s) 1708
BstENI CCTNNNNNAGG 2 cut(s) 224, 496
BstF5I GGATG 9 cut(s) 5, 70, 368, 490, 772, 835, 979, 1459, 2245
BstKTI GATC 9 cut(s) 317, 442, 451, 619, 736, 963, 1490, 1913, 1954
BstMAI GTCTC 2 cut(s) 66, 1706
BstMBI GATC 9 cut(s) 314, 439, 448, 616, 733, 960, 1487, 1910, 1951
BstMWI GCNNNNNNNGC 3 cut(s) 283, 699, 2169
BstNI CCWGG 3 cut(s) 2145, 2160, 2193
BstNSI RCATGY 3 cut(s) 200, 1074, 1150
BstSCI CCNGG 3 cut(s) 2143, 2158, 2191
BstSFI CTRYAG 2 cut(s) 780, 1745
BstV1I GCAGC 5 cut(s) 1076, 1731, 1759, 1870, 2051
BstV2I GAAGAC 4 cut(s) 1370, 1616, 1640, 2004
BstX2I RGATCY 2 cut(s) 1487, 1910
BstXI CCANNNNNNTGG 2 cut(s) 511, 1248
BstYI RGATCY 2 cut(s) 1487, 1910
Bsu36I CCTNAGG 1 cut(s) 761
BsuRI GGCC 3 cut(s) 1707, 2025, 2196
BtgI CCRYGG 1 cut(s) 1708
BtgZI GCGATG 1 cut(s) 735
BtsCI GGATG 9 cut(s) 5, 70, 368, 490, 772, 835, 979, 1459, 2245
BtsIMutI CAGTG 4 cut(s) 685, 893, 1647, 1803
BveI ACCTGC 1 cut(s) 2219
Cac8I GCNNGC 3 cut(s) 537, 1148, 1181
Cfr13I GGNCC 7 cut(s) 310, 501, 765, 911, 1330, 2141, 2195
CpoI CGGWCCG 1 cut(s) 310
CseI GACGC 1 cut(s) 853
Csp6I GTAC 4 cut(s) 248, 387, 609, 1165
CspCI CAANNNNNGTGG 2 cut(s) 1792, 1827
CspI CGGWCCG 1 cut(s) 310
CviQI GTAC 4 cut(s) 248, 387, 609, 1165
DpnI GATC 9 cut(s) 316, 441, 450, 618, 735, 962, 1489, 1912, 1953
DpnII GATC 9 cut(s) 314, 439, 448, 616, 733, 960, 1487, 1910, 1951
DraIII CACNNNGTG 1 cut(s) 989
DrdI GACNNNNNNGTC 1 cut(s) 1402
DseDI GACNNNNNNGTC 1 cut(s) 1402
EaeI YGGCCR 2 cut(s) 1705, 2023
Eam1104I CTCTTC 4 cut(s) 287, 347, 1691, 1768
EarI CTCTTC 4 cut(s) 287, 347, 1691, 1768
Ecl136II GAGCTC 1 cut(s) 1662
Eco130I CCWWGG 6 cut(s) 225, 255, 825, 1449, 1708, 2167
Eco24I GRGCYC 1 cut(s) 1664
Eco47I GGWCC 6 cut(s) 310, 501, 765, 911, 1330, 2141
Eco53kI GAGCTC 1 cut(s) 1662
Eco57I CTGAAG 3 cut(s) 302, 912, 1628
Eco72I CACGTG 1 cut(s) 1932
Eco81I CCTNAGG 1 cut(s) 761
Eco88I CYCGRG 1 cut(s) 236
EcoICRI GAGCTC 1 cut(s) 1662
EcoNI CCTNNNNNAGG 2 cut(s) 224, 496
EcoO109I RGGNCCY 2 cut(s) 765, 911
EcoRII CCWGG 3 cut(s) 2143, 2158, 2191
EcoT14I CCWWGG 6 cut(s) 225, 255, 825, 1449, 1708, 2167
EcoT38I GRGCYC 1 cut(s) 1664
ErhI CCWWGG 6 cut(s) 225, 255, 825, 1449, 1708, 2167
Esp3I CGTCTC 1 cut(s) 66
FaqI GGGAC 3 cut(s) 897, 1343, 1390
FauI CCCGC 2 cut(s) 438, 544
FblI GTMKAC 1 cut(s) 788
Fnu4HI GCNGC 5 cut(s) 1090, 1745, 1748, 1859, 2040
FokI GGATG 7 cut(s) 77, 355, 497, 759, 842, 966, 1466
FriOI GRGCYC 1 cut(s) 1664
Fsp4HI GCNGC 5 cut(s) 1090, 1745, 1748, 1859, 2040
FspBI CTAG 2 cut(s) 908, 1872
GluI GCNGC 5 cut(s) 1090, 1745, 1748, 1859, 2040
GsaI CCCAGC 1 cut(s) 1888
GsuI CTGGAG 3 cut(s) 556, 1623, 2166
HaeIII GGCC 3 cut(s) 1707, 2025, 2196
HapII CCGG 1 cut(s) 1996
HgaI GACGC 1 cut(s) 853
Hin1I GRCGYC 1 cut(s) 420
HincII GTYRAC 2 cut(s) 1903, 2131
HindII GTYRAC 2 cut(s) 1903, 2131
HpaI GTTAAC 1 cut(s) 2131
HpaII CCGG 1 cut(s) 1996
HphI GGTGA 8 cut(s) 131, 526, 743, 1588, 1609, 1658, 1691, 2192
Hpy166II GTNNAC 8 cut(s) 387, 789, 1100, 1649, 1801, 1903, 2131, 2156
Hpy188III TCNNGA 5 cut(s) 362, 1010, 1949, 1979, 2055
Hpy8I GTNNAC 8 cut(s) 387, 789, 1100, 1649, 1801, 1903, 2131, 2156
Hpy99I CGWCG 1 cut(s) 847
HpyAV CCTTC 8 cut(s) 110, 350, 599, 743, 1111, 1247, 1304, 1676
HpyCH4III ACNGT 9 cut(s) 43, 166, 310, 781, 793, 869, 1369, 1565, 2135
HpyCH4IV ACGT 3 cut(s) 420, 466, 1931
HpyF10VI GCNNNNNNNGC 3 cut(s) 283, 699, 2169
HpySE526I ACGT 3 cut(s) 420, 466, 1931
Hsp92I GRCGYC 1 cut(s) 420
KspAI GTTAAC 1 cut(s) 2131
Kzo9I GATC 9 cut(s) 314, 439, 448, 616, 733, 960, 1487, 1910, 1951
LguI GCTCTTC 1 cut(s) 287
LmnI GCTCC 3 cut(s) 1659, 1667, 2194
Lsp1109I GCAGC 5 cut(s) 1076, 1731, 1759, 1870, 2051
LweI GCATC 4 cut(s) 14, 478, 829, 1601
MaeI CTAG 2 cut(s) 908, 1872
MaeII ACGT 3 cut(s) 420, 466, 1931
MaeIII GTNAC 4 cut(s) 680, 1369, 1468, 1576
MalI GATC 9 cut(s) 316, 441, 450, 618, 735, 962, 1489, 1912, 1953
MboI GATC 9 cut(s) 314, 439, 448, 616, 733, 960, 1487, 1910, 1951
MflI RGATCY 2 cut(s) 1487, 1910
MhlI GDGCHC 1 cut(s) 1664
MlsI TGGCCA 1 cut(s) 2025
MluNI TGGCCA 1 cut(s) 2025
MlyI GAGTC 6 cut(s) 683, 794, 1412, 1630, 1708, 2054
MmeI TCCRAC 1 cut(s) 800
Mox20I TGGCCA 1 cut(s) 2025
MroXI GAANNNNTTC 1 cut(s) 1613
MscI TGGCCA 1 cut(s) 2025
MseI TTAA 5 cut(s) 144, 1235, 1337, 2103, 2130
MslI CAYNNNNRTG 1 cut(s) 2092
Msp20I TGGCCA 1 cut(s) 2025
MspA1I CMGCKG 1 cut(s) 2042
MspI CCGG 1 cut(s) 1996
MspR9I CCNGG 3 cut(s) 2145, 2160, 2193
Mva1269I GAATGC 4 cut(s) 427, 600, 1272, 1828
MvaI CCWGG 3 cut(s) 2145, 2160, 2193
MwoI GCNNNNNNNGC 3 cut(s) 283, 699, 2169
NcoI CCATGG 1 cut(s) 1708
NdeII GATC 9 cut(s) 314, 439, 448, 616, 733, 960, 1487, 1910, 1951
NlaIV GGNNCC 5 cut(s) 758, 913, 1331, 1882, 2079
NmeAIII GCCGAG 2 cut(s) 312, 1683
NmuCI GTSAC 2 cut(s) 680, 1576
NspI RCATGY 3 cut(s) 200, 1074, 1150
PaeI GCATGC 1 cut(s) 1150
PciI ACATGT 1 cut(s) 1070
PciSI GCTCTTC 1 cut(s) 287
PctI GAATGC 4 cut(s) 427, 600, 1272, 1828
PdmI GAANNNNTTC 1 cut(s) 1613
PfeI GAWTC 4 cut(s) 178, 470, 1078, 1982
PflFI GACNNNGTC 1 cut(s) 308
PflMI CCANNNNNTGG 2 cut(s) 61, 108
PfoI TCCNGGA 1 cut(s) 2143
PkrI GCNGC 5 cut(s) 1091, 1746, 1749, 1860, 2041
PleI GAGTC 6 cut(s) 682, 793, 1411, 1630, 1707, 2053
PmaCI CACGTG 1 cut(s) 1932
PmlI CACGTG 1 cut(s) 1932
PpsI GAGTC 6 cut(s) 682, 793, 1411, 1630, 1707, 2053
Ppu21I YACGTR 2 cut(s) 467, 1932
PpuMI RGGWCCY 2 cut(s) 765, 911
PscI ACATGT 1 cut(s) 1070
PshBI ATTAAT 1 cut(s) 1235
PsiI TTATAA 1 cut(s) 1325
Psp124BI GAGCTC 1 cut(s) 1664
Psp5II RGGWCCY 2 cut(s) 765, 911
Psp6I CCWGG 3 cut(s) 2143, 2158, 2191
PspCI CACGTG 1 cut(s) 1932
PspFI CCCAGC 1 cut(s) 1884
PspGI CCWGG 3 cut(s) 2143, 2158, 2191
PspN4I GGNNCC 5 cut(s) 758, 913, 1331, 1882, 2079
PspPI GGNCC 7 cut(s) 310, 501, 765, 911, 1330, 2141, 2195
PspPPI RGGWCCY 2 cut(s) 765, 911
PstI CTGCAG 1 cut(s) 1749
PsuI RGATCY 2 cut(s) 1487, 1910
PsyI GACNNNGTC 1 cut(s) 308
PvuII CAGCTG 1 cut(s) 2042
RsaI GTAC 4 cut(s) 249, 388, 610, 1166
RsaNI GTAC 4 cut(s) 248, 387, 609, 1165
RseI CAYNNNNRTG 1 cut(s) 2092
Rsr2I CGGWCCG 1 cut(s) 310
RsrII CGGWCCG 1 cut(s) 310
SacI GAGCTC 1 cut(s) 1664
SapI GCTCTTC 1 cut(s) 287
SaqAI TTAA 5 cut(s) 144, 1235, 1337, 2103, 2130
SatI GCNGC 5 cut(s) 1090, 1745, 1748, 1859, 2040
Sau3AI GATC 9 cut(s) 314, 439, 448, 616, 733, 960, 1487, 1910, 1951
Sau96I GGNCC 7 cut(s) 310, 501, 765, 911, 1330, 2141, 2195
SchI GAGTC 6 cut(s) 683, 794, 1412, 1630, 1708, 2054
ScrFI CCNGG 3 cut(s) 2145, 2160, 2193
SduI GDGCHC 1 cut(s) 1664
SfaNI GCATC 4 cut(s) 14, 478, 829, 1601
SfcI CTRYAG 2 cut(s) 780, 1745
SinI GGWCC 6 cut(s) 310, 501, 765, 911, 1330, 2141
SmiMI CAYNNNNRTG 1 cut(s) 2092
SmlI CTYRAG 1 cut(s) 1037
SmoI CTYRAG 1 cut(s) 1037
SphI GCATGC 1 cut(s) 1150
SsiI CCGC 3 cut(s) 445, 537, 2008
SspI AATATT 1 cut(s) 2101
SspMI CTAG 2 cut(s) 908, 1872
SstI GAGCTC 1 cut(s) 1664
StyD4I CCNGG 3 cut(s) 2143, 2158, 2191
StyI CCWWGG 6 cut(s) 225, 255, 825, 1449, 1708, 2167
TaaI ACNGT 9 cut(s) 43, 166, 310, 781, 793, 869, 1369, 1565, 2135
TaiI ACGT 3 cut(s) 423, 469, 1934
TaqI TCGA 2 cut(s) 970, 1009
TatI WGTACW 2 cut(s) 247, 386
TfiI GAWTC 4 cut(s) 178, 470, 1078, 1982
Tru1I TTAA 5 cut(s) 144, 1235, 1337, 2103, 2130
Tru9I TTAA 5 cut(s) 144, 1235, 1337, 2103, 2130
TscAI CASTG 4 cut(s) 685, 893, 1647, 1803
TseFI GTSAC 2 cut(s) 680, 1576
TseI GCWGC 5 cut(s) 1089, 1744, 1747, 1858, 2039
Tsp45I GTSAC 2 cut(s) 680, 1576
TspRI CASTG 4 cut(s) 685, 893, 1647, 1803
Tth111I GACNNNGTC 1 cut(s) 308
Van91I CCANNNNNTGG 2 cut(s) 61, 108
VpaK11BI GGWCC 6 cut(s) 310, 501, 765, 911, 1330, 2141
VspI ATTAAT 1 cut(s) 1235
XagI CCTNNNNNAGG 2 cut(s) 224, 496
XapI RAATTY 3 cut(s) 517, 1340, 1389
XceI RCATGY 3 cut(s) 200, 1074, 1150
XmiI GTMKAC 1 cut(s) 788
XmnI GAANNNNTTC 1 cut(s) 1613
XspI CTAG 2 cut(s) 908, 1872
ZraI GACGTC 1 cut(s) 421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.