Rh4AG130100

metal ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000343
Physical Location & Seq
Forward (+)
28214208 .. 28226452
12245 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG130100.1

Sequence Viewer

Length: 2946 bp
ATGGAATCGGGTTCTTCTCTGAATTTGGTCAGGTGTGAGTTAAACAAGGGTATGGCATCGGCAGAGTGTCAACCATATTCTGTTTCGGAGTACCATTTCGTAGATGACAAGGATGAATGTGTCTCATTTCATGTATTGCCTGTTCAATGGAGTAATGGTGAGAGACTGGATGCCGAGAAAGTAAAGATTTTTCTGCATGGAACCGCTGATAATGGGGGGAATTTATGTAAGCATGTTGTTGCATGGAAGTTTGACATTTCGAGTGAAGAGCCCGAGATATTGGTTCTCTCTGAGGAAAATAGTTGGATCAAGCTTGGGAAGCCGAGGAAGAGCTTTGAGGACACTGTTAGGTCGATCTTGATAACGGTGCACTGCCTTCACTATGTGAAGAGAAATCCTGAAAAATCCAGAGAATGTGTGTGGAATCACCTATCTGAAGTTTTCAGGTTGTATGTGGTCAGGCCTTCTCAAAGTGATCTGGTGGATCACATGCCTCTAATTGGTGATTGTGTTAACAGATATCGTGCCCTAGCGGATTCCAAGTTTTTGGCCAAACTTTTTGAAGAGAAGACAATGAAGAGGAAACTCTCTGATGAGGATACTGAAGCTAGAAAGAAGCTCAGAATTATGATCGATGATGTAAAAGATGATATGATTCATGCAGAAGATGAGGATATCGATAACCTGTTTGGTGAGGTCTGTGCTTTATGTGACGATGGTGGCAAGATCATTTGTTGTGAAGGGGGGTGCTTAAGGTCTTTTCATGCAACTGAGGAGGCGGGTGCCGGATCTTATTGCAAATCTCTTGGCTTTATGCAGGATGATGTTGTTGGGATGCAAAATTTCTACTGCAAGAACTGTGAATATAAACAGCACCAGTGCTTTGTTTGTGGGAAGTTAGGTTCCTCTGATAAGTCCTCAGGTGCTGCTGAGGTGGTTTCCTGTGTATCCCCAAACTGTGGTCGATTTTACCATCCACATTGTGTTGCAATATTACTTTACCAAGATAATGGAGTTCCTGCTGAAGAACTTGAGAGAAAGATTGCTGGTGGGGAATCTTTTACTTGTCCACTTCATAAGTGCTGCATTTGTAAACAAGGAGAAAATAAGAAGGATAGTGAGTTACAATTTGCTGTGTGCATGCATTGTCCAAAATCGTACCACAGGAAATGCCTGCCAAGTGAGATTGCTTTTGAAAATCAAGGAGAAATGCTAGAAGGGAGATCAATAATAAGAGCTTGGGATGGTCTATTACCTAACCGTATACTCATATATTGCACAAAACATGATATAGATGTTGAATTTGGGACTGTAAGAAGGGACCACGTAAAATTCCCTGATGTCAAACAGAATATGAGCACTCTCAAAAAGAAGAAGAAGACTATTTTAGAAGAGAAGTGGAAGCCAATATCAGAATTCCTTGTAGACGGAGACAAAGTTGGGACTAAGAGAAATATTTCCTTGGAAAAATCATGTAGGGATAAAAATGCTCCTACAGCACTTAAGACACAAAAGCCATATGTTCAAGAGAAGAAGACTAGGAAACTTATGTCAGGGCAGAAACCTGTGTCTAAGAAGAGCGATTCTTCTTTGGAAGAATCAGTTGGGGAAGGAAGTGATCCTGTACTATCCACACTAGTCAAGCAGAAGCTTTATTCAGTTAAAGAGAAGAAGGCTACTTTTGTAAAAGAGAAAACTTTTGTTGAAGAGAAAGAAAGCCAACTGACATCAACGGTCTCTGTAGACAGAGAAAAAGCTGTGTTGAAGAGGAGAACTCCTTCCTCTGAAGGACTATATAGTGAGAAAAGTGCTCCTACAATATCCAAGAAAAAACAGAAGCCATCTTTTGTTGAAGAGAAGAACAATGACCTTGTAAAAAAGAAAACTAATGTTGAAGAGAAGAGGAAACAGACATCAGAGTCCCTTGTTGAGAGAGAGACAGCTGTGTCCAAAAAGAAAAATCCTTCCTCGAAAGAATCCTATAGGGAAAGAAATGCTTCTACAACTCCCAAACAGAAGCAGAAGCCATCAGAGCTCACTGTAGATGGAGATGAGGCTGGGTCTAAGAAGGGAAGTCCTTTGGAAGAATCATTACGTGCAATTGCTTCAACTGTACCCAAACTGAAGCAGAAGTCAACTTCTGCCGTGAAACTGGGTAGTAACAAGAATAGGGAAAAGACGTTTTCCGGTATAGATATTTCAAGGATGGTGAAACGAAATAATTCACTGAAAAACGAACGTCAGGCTTCCATGTCTGAGAAAATCAAGACCTCACTGGGTGAAGAGCCATTTTCTTCAATGGAGTCTGAGCAGGTGAAGCTCGGGAACCTGGATACACCTGATGCTAAAACAGTTAGTGAGATACACAGGGAAATGCCACCACATACTGAATGTGGTGACGATGGAAATCAGCATCCTAACCCCAGCAAGTCTCGTATGCATTGTGCTCATGCTAACGGTCGAGTCCAATTCAAAATTCCTGATGATGCAGGCAGAAGGTGCACCACGAGTAATGATGAGCCTCGTTCAAGTGTTACCAATCCTAGGTCTGGCAATGCGAAAGGGTGCAAGAGAGAGAACTCGGGAAAACCATATTCAAATGGGGGGATGAAACAGAAACAGAAACTGAGAACAGAATCTGACAGAGTCCCACCGGCTTGCATTAAAGGGAAGCAAGATCCTTTTTTTTCAAGAGGCAATGATCTAGCGGGCCAAAATTCAGTATATGACCATATTAGGACGTTCTCATCTACTTTTTACAGCCATACAGGTCCTGTTGCTGACTCGTTATCTAGTATCAACAGATTAGCTATGCCATCAGATGCAACTAAATTGGTTGGTCCAAGAGCACCCCAACCTGATTACCTGGATAGGTGTATGGGTTTCGCTCCTGGTCCGCACCTGAACTATTCACTACAGAATTCAGCTGGTTGGATCGATGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006325 GO:0006355 GO:0006950 GO:0006952 GO:0006996 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0009605 GO:0009607 GO:0009620 GO:0009791 GO:0009889 GO:0009893 GO:0009909 GO:0009911 GO:0009987 GO:0010228 GO:0010468 GO:0010556 GO:0010604 GO:0010638 GO:0016043 GO:0019219 GO:0019222 GO:0022414 GO:0023052 GO:0030154 GO:0031056 GO:0031058 GO:0031060 GO:0031062 GO:0031323 GO:0031325 GO:0031326 GO:0031399 GO:0031401 GO:0031440 GO:0031935 GO:0031937 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033043 GO:0033044 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0043954 GO:0044030 GO:0044424 GO:0044464 GO:0048366 GO:0048367 GO:0048468 GO:0048518 GO:0048522 GO:0048580 GO:0048582 GO:0048608 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0048869 GO:0050684 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051052 GO:0051094 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051276 GO:0051570 GO:0051574 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060968 GO:0061085 GO:0061087 GO:0061458 GO:0065007 GO:0070827 GO:0070828 GO:0070829 GO:0071840 GO:0080090 GO:0090436 GO:0097159 GO:0098542 GO:0099402 GO:0140110 GO:1900109 GO:1900111 GO:1900363 GO:1901363 GO:1902275 GO:1903311 GO:1903506 GO:1905269 GO:2000024 GO:2000026 GO:2000112 GO:2000241 GO:2000243 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNMT1-RFD

Protein Analysis

981

Amino Acids

109.95

Weight (kDa)

8.71

Isoelectric Point (pI)

49.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNMT1-RFD PF12047 28 - 149 2.8e-10 Cytosine specific DNA methyltransferase replication foci domain
PHD_NSD PF22908 290 - 359 3.4e-19 Histone-lysine N-methyltransferase NSD-like, PHD zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000343)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48090 AT5G48090 AT5G48090 AT5G55390 AT5G55390 AT5G55390
fragaria_vesca FvH4_3g23690 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160
malus_domestica MD03G1191000.v1.1 MD11G1207800.v1.1 MD11G1208000.v1.1
prunus_persica Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227600_v2.0.a1 Prupe.4G227700_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1
pyrus_communis pycom01g03400 pycom03g14420 pycom11g18100 pycom11g18140 pycom11g18150 pycom11g18160
rosa_chinensis RchiOBHm_Chr2g0145981 RchiOBHm_Chr4g0405241 RchiOBHm_Chr4g0405271 RchiOBHm_Chr5g0016201 RchiOBHm_Chr5g0041831 RchiOBHm_Chr5g0041871 RchiOBHm_Chr5g0041881 RchiOBHm_Chr5g0059241
rosa_laevigata RLG00000002117 RLG00000008861 RLG00000008862 RLG00000011000 RLG00000033216 RLG00000034097 RLG00000034101
rosa_multiflora Rmu_sc0000161.1_g000001 Rmu_sc0000861.1_g000040 Rmu_sc0007522.1_g000003 Rmu_sc0009443.1_g000015 Rmu_sc0022129.1_g000001 Rmu_sc0042315.1_g000001
rosa_roxburghii Rroxscaffold_1G00038660 Rroxscaffold_1G00038690 Rroxscaffold_5G00349560 Rroxscaffold_5G00349610 Rroxscaffold_6G00388370 Rroxscaffold_7G00188740
rosa_rugosa Rorug03G0199700 Rorug04G0055400 Rorug04G0055500 Rorug04G0055900 Rorug04G0056000 Rorug04G0056100 Rorug04G0056100 Rorug04G0056200 Rorug05G0195600 Rorug05G0195700 Rorug05G0195800 Rorug05G0195900 Rorug05G0196800 Rorug05G0385000
rosa_samantha Rh4AG129900 Rh4AG130100 Rh4BG124200 Rh4BG124400 Rh4BG124500 Rh4CG137200 Rh4CG137300 Rh4DG123500 Rh4DG123700 Rh5AG280800 Rh5AG281400 Rh5BG286700 Rh5BG287000 Rh5BG397800 Rh5CG318100 Rh5CG318600 Rh5CG421600 Rh5DG132300 Rh5DG132400 Rh5DG295400 Rh5DG295700 Rh7BG475300
rosa_wichuraiana Rw0G001330 Rw0G005730 Rw4G008670 Rw4G010510 Rw4G010540 Rw5G026450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2302
AasI GACNNNNNNGTC 2 cut(s) 1918, 1945
Acc36I ACCTGC 1 cut(s) 2302
AccB1I GGYRCC 1 cut(s) 782
AccB7I CCANNNNNTGG 1 cut(s) 959
AccI GTMKAC 3 cut(s) 1264, 1425, 1743
AciI CCGC 5 cut(s) 204, 533, 779, 2708, 2897
AclWI GGATC 6 cut(s) 314, 492, 796, 1613, 2672, 2942
AcoI YGGCCR 1 cut(s) 549
AcsI RAATTY 9 cut(s) 22, 220, 841, 1301, 1331, 1415, 2475, 2716, 2920
AcuI CTGAAG 5 cut(s) 456, 624, 1044, 1806, 2144
AdeI CACNNNGTG 3 cut(s) 385, 983, 2279
AfaI GTAC 4 cut(s) 92, 1160, 1626, 2115
AfiI CCNNNNNNNGG 4 cut(s) 500, 959, 2544, 2549
AflII CTTAAG 2 cut(s) 751, 1502
AhlI ACTAGT 1 cut(s) 1636
AjnI CCWGG 3 cut(s) 2328, 2865, 2890
AjuI GAANNNNNNNTTGG 4 cut(s) 1587, 1619, 2460, 2492
AloI GAACNNNNNNTCC 2 cut(s) 886, 918
Alw21I GWGCWC 7 cut(s) 372, 1361, 1813, 2037, 2449, 2504, 2851
Alw26I GTCTC 6 cut(s) 127, 157, 1425, 1741, 1931, 2436
Alw44I GTGCAC 2 cut(s) 368, 2500
AlwI GGATC 6 cut(s) 314, 492, 796, 1613, 2672, 2942
AlwNI CAGNNNCTG 4 cut(s) 926, 2626, 2639, 2774
Ama87I CYCGRG 3 cut(s) 272, 2321, 2581
AoxI GGCC 3 cut(s) 461, 549, 2710
ApaLI GTGCAC 2 cut(s) 368, 2500
ApeKI GCWGC 2 cut(s) 926, 1083
ApoI RAATTY 9 cut(s) 22, 220, 841, 1301, 1331, 1415, 2475, 2716, 2920
Asp700I GAANNNNTTC 4 cut(s) 1456, 1777, 1996, 2221
AspA2I CCTAGG 1 cut(s) 2543
AspS9I GGNCC 5 cut(s) 1321, 2710, 2771, 2840, 2894
AsuHPI GGTGA 8 cut(s) 170, 419, 515, 704, 2221, 2291, 2326, 2408
AvaI CYCGRG 3 cut(s) 272, 2321, 2581
AvaII GGWCC 4 cut(s) 1321, 2771, 2840, 2894
AvrII CCTAGG 1 cut(s) 2543
AxyI CCTNAGG 1 cut(s) 919
BaeGI GKGCMC 3 cut(s) 372, 529, 2504
BalI TGGCCA 1 cut(s) 551
BanI GGYRCC 1 cut(s) 782
BanII GRGCYC 2 cut(s) 273, 2037
BarI GAAGNNNNNNTAC 2 cut(s) 2076, 2108
BauI CACGAG 1 cut(s) 2506
BbsI GAAGAC 3 cut(s) 575, 1385, 1541
Bbv12I GWGCWC 7 cut(s) 372, 1361, 1813, 2037, 2449, 2504, 2851
BbvCI CCTCAGC 1 cut(s) 930
BbvI GCAGC 2 cut(s) 913, 1070
BccI CCATC 9 cut(s) 710, 981, 1238, 1849, 2035, 2039, 2200, 2396, 2824
BceAI ACGGC 1 cut(s) 2129
BciT130I CCWGG 3 cut(s) 2330, 2867, 2892
BciVI GTATCC 3 cut(s) 592, 958, 2326
BcoDI GTCTC 6 cut(s) 127, 157, 1425, 1741, 1931, 2436
BcuI ACTAGT 1 cut(s) 1636
BfaI CTAG 8 cut(s) 530, 609, 1214, 1539, 1637, 2544, 2705, 2793
BfmI CTRYAG 5 cut(s) 1494, 1740, 1981, 2040, 2915
BfrI CTTAAG 2 cut(s) 751, 1502
BfuAI ACCTGC 1 cut(s) 2302
BfuI GTATCC 3 cut(s) 592, 958, 2326
BisI GCNGC 2 cut(s) 927, 1084
BlnI CCTAGG 1 cut(s) 2543
BlsI GCNGC 2 cut(s) 928, 1085
Bme1390I CCNGG 3 cut(s) 2330, 2867, 2892
Bme18I GGWCC 4 cut(s) 1321, 2771, 2840, 2894
BmeT110I CYCGRG 3 cut(s) 272, 2321, 2581
BmgT120I GGNCC 5 cut(s) 1321, 2710, 2771, 2840, 2894
BmiI GGNNCC 5 cut(s) 202, 784, 904, 1322, 2327
BmrFI CCNGG 3 cut(s) 2330, 2867, 2892
BmrI ACTGGG 2 cut(s) 2162, 2285
BmsI GCATC 7 cut(s) 65, 160, 825, 2332, 2422, 2476, 2812
BmuI ACTGGG 2 cut(s) 2162, 2285
BpiI GAAGAC 3 cut(s) 575, 1385, 1541
BplI GAGNNNNNCTC 2 cut(s) 1759, 1791
Bpu10I CCTNAGC 1 cut(s) 930
BpuEI CTTGAG 1 cut(s) 1052
Bsa29I ATCGAT 3 cut(s) 633, 678, 2937
BsaAI YACGTR 2 cut(s) 1327, 2096
BsaBI GATNNNNATC 1 cut(s) 2406
BsaI GGTCTC 1 cut(s) 1741
BsaJI CCNNGG 3 cut(s) 323, 1461, 2543
BsaWI WCCGGW 1 cut(s) 2186
BsaXI ACNNNNNCTCC 4 cut(s) 142, 172, 1422, 1452
Bsc4I CCNNNNNNNGG 4 cut(s) 500, 959, 2544, 2549
Bse118I RCCGGY 1 cut(s) 2653
Bse1I ACTGG 4 cut(s) 171, 877, 2157, 2280
Bse21I CCTNAGG 1 cut(s) 919
Bse3DI GCAATG 2 cut(s) 2560, 2704
Bse8I GATNNNNATC 1 cut(s) 2406
BseBI CCWGG 3 cut(s) 2330, 2867, 2892
BseCI ATCGAT 3 cut(s) 633, 678, 2937
BseDI CCNNGG 3 cut(s) 323, 1461, 2543
BseGI GGATG 9 cut(s) 118, 175, 826, 840, 973, 1249, 2211, 2413, 2613
BseJI GATNNNNATC 1 cut(s) 2406
BseLI CCNNNNNNNGG 4 cut(s) 500, 959, 2544, 2549
BseMI GCAATG 2 cut(s) 2560, 2704
BseMII CTCAG 8 cut(s) 282, 634, 762, 921, 933, 2247, 2298, 2618
BseNI ACTGG 4 cut(s) 171, 877, 2157, 2280
BseRI GAGGAG 2 cut(s) 788, 1783
BseSI GKGCMC 3 cut(s) 372, 529, 2504
BseXI GCAGC 2 cut(s) 913, 1070
BseYI CCCAGC 2 cut(s) 2057, 2423
Bsh1285I CGRYCG 1 cut(s) 2461
BshFI GGCC 3 cut(s) 463, 551, 2712
BshNI GGYRCC 1 cut(s) 782
BshVI ATCGAT 3 cut(s) 633, 678, 2937
BsiEI CGRYCG 1 cut(s) 2461
BsiHKAI GWGCWC 7 cut(s) 372, 1361, 1813, 2037, 2449, 2504, 2851
BsiHKCI CYCGRG 3 cut(s) 272, 2321, 2581
BsiSI CCGG 3 cut(s) 786, 2187, 2654
BslFI GGGAC 5 cut(s) 1321, 1334, 1456, 1906, 2633
BslI CCNNNNNNNGG 4 cut(s) 500, 959, 2544, 2549
BsmAI GTCTC 6 cut(s) 127, 157, 1425, 1741, 1931, 2436
BsmFI GGGAC 5 cut(s) 1321, 1334, 1456, 1906, 2633
BsnI GGCC 3 cut(s) 463, 551, 2712
Bso31I GGTCTC 1 cut(s) 1741
BsoBI CYCGRG 3 cut(s) 272, 2321, 2581
Bsp1286I GDGCHC 9 cut(s) 273, 372, 529, 1361, 1813, 2037, 2449, 2504, 2851
BspACI CCGC 5 cut(s) 204, 533, 779, 2708, 2897
BspANI GGCC 3 cut(s) 463, 551, 2712
BspCNI CTCAG 8 cut(s) 283, 633, 763, 922, 932, 2248, 2299, 2619
BspDI ATCGAT 3 cut(s) 633, 678, 2937
BspLI GGNNCC 5 cut(s) 202, 784, 904, 1322, 2327
BspMI ACCTGC 1 cut(s) 2302
BspPI GGATC 6 cut(s) 314, 492, 796, 1613, 2672, 2942
BspQI GCTCTTC 4 cut(s) 261, 323, 1571, 2277
BspT107I GGYRCC 1 cut(s) 782
BspTI CTTAAG 2 cut(s) 751, 1502
BspTNI GGTCTC 1 cut(s) 1741
BsrDI GCAATG 2 cut(s) 2560, 2704
BsrFI RCCGGY 1 cut(s) 2653
BsrI ACTGG 4 cut(s) 171, 877, 2157, 2280
BssAI RCCGGY 1 cut(s) 2653
BssECI CCNNGG 3 cut(s) 323, 1461, 2543
BssNAI GTATAC 1 cut(s) 1265
BssSI CACGAG 1 cut(s) 2506
BssT1I CCWWGG 2 cut(s) 1461, 2543
Bst1107I GTATAC 1 cut(s) 1265
Bst2BI CACGAG 1 cut(s) 2506
Bst2UI CCWGG 3 cut(s) 2330, 2867, 2892
BstAFI CTTAAG 2 cut(s) 751, 1502
BstAPI GCANNNNNTGC 1 cut(s) 2499
BstBAI YACGTR 2 cut(s) 1327, 2096
BstC8I GCNNGC 5 cut(s) 1142, 1175, 2491, 2659, 2710
BstF5I GGATG 9 cut(s) 118, 175, 826, 840, 973, 1249, 2211, 2413, 2613
BstMAI GTCTC 6 cut(s) 127, 157, 1425, 1741, 1931, 2436
BstMCI CGRYCG 1 cut(s) 2461
BstMWI GCNNNNNNNGC 5 cut(s) 319, 1496, 2032, 2317, 2499
BstNI CCWGG 3 cut(s) 2330, 2867, 2892
BstNSI RCATGY 3 cut(s) 236, 493, 1144
BstSCI CCNGG 3 cut(s) 2328, 2865, 2890
BstSFI CTRYAG 5 cut(s) 1494, 1740, 1981, 2040, 2915
BstSLI GKGCMC 3 cut(s) 372, 529, 2504
BstV1I GCAGC 2 cut(s) 913, 1070
BstV2I GAAGAC 3 cut(s) 575, 1385, 1541
BstX2I RGATCY 2 cut(s) 788, 2677
BstXI CCANNNNNNTGG 2 cut(s) 547, 1010
BstYI RGATCY 2 cut(s) 788, 2677
BstZ17I GTATAC 1 cut(s) 1265
Bsu15I ATCGAT 3 cut(s) 633, 678, 2937
Bsu36I CCTNAGG 1 cut(s) 919
BsuI GTATCC 3 cut(s) 592, 958, 2326
BsuRI GGCC 3 cut(s) 463, 551, 2712
BsuTUI ATCGAT 3 cut(s) 633, 678, 2937
BtsCI GGATG 9 cut(s) 118, 175, 826, 840, 973, 1249, 2211, 2413, 2613
BtsI GCAGTG 1 cut(s) 370
BtsIMutI CAGTG 6 cut(s) 342, 370, 884, 2037, 2225, 2273
BveI ACCTGC 1 cut(s) 2302
Cac8I GCNNGC 5 cut(s) 1142, 1175, 2491, 2659, 2710
CaiI CAGNNNCTG 4 cut(s) 926, 2626, 2639, 2774
Cfr10I RCCGGY 1 cut(s) 2653
Cfr13I GGNCC 5 cut(s) 1321, 2710, 2771, 2840, 2894
ClaI ATCGAT 3 cut(s) 633, 678, 2937
Csp6I GTAC 4 cut(s) 91, 1159, 1625, 2114
CviQI GTAC 4 cut(s) 91, 1159, 1625, 2114
DraIII CACNNNGTG 3 cut(s) 385, 983, 2279
DrdI GACNNNNNNGTC 2 cut(s) 1918, 1945
DseDI GACNNNNNNGTC 2 cut(s) 1918, 1945
EaeI YGGCCR 1 cut(s) 549
Ecl136II GAGCTC 1 cut(s) 2035
Eco130I CCWWGG 2 cut(s) 1461, 2543
Eco147I AGGCCT 1 cut(s) 463
Eco24I GRGCYC 2 cut(s) 273, 2037
Eco31I GGTCTC 1 cut(s) 1741
Eco32I GATATC 2 cut(s) 521, 676
Eco47I GGWCC 4 cut(s) 1321, 2771, 2840, 2894
Eco53kI GAGCTC 1 cut(s) 2035
Eco57I CTGAAG 5 cut(s) 456, 624, 1044, 1806, 2144
Eco81I CCTNAGG 1 cut(s) 919
Eco88I CYCGRG 3 cut(s) 272, 2321, 2581
EcoICRI GAGCTC 1 cut(s) 2035
EcoO109I RGGNCCY 1 cut(s) 2771
EcoRI GAATTC 2 cut(s) 1415, 2920
EcoRII CCWGG 3 cut(s) 2328, 2865, 2890
EcoRV GATATC 2 cut(s) 521, 676
EcoT14I CCWWGG 2 cut(s) 1461, 2543
EcoT22I ATGCAT 2 cut(s) 1146, 2442
EcoT38I GRGCYC 2 cut(s) 273, 2037
ErhI CCWWGG 2 cut(s) 1461, 2543
FalI AAGNNNNNCTT 2 cut(s) 1981, 2013
FaqI GGGAC 5 cut(s) 1321, 1334, 1456, 1906, 2633
FauI CCCGC 2 cut(s) 772, 2701
FauNDI CATATG 1 cut(s) 1519
FblI GTMKAC 3 cut(s) 1264, 1425, 1743
Fnu4HI GCNGC 2 cut(s) 927, 1084
FokI GGATG 9 cut(s) 125, 182, 833, 847, 960, 1256, 2218, 2400, 2620
FriOI GRGCYC 2 cut(s) 273, 2037
Fsp4HI GCNGC 2 cut(s) 927, 1084
FspBI CTAG 8 cut(s) 530, 609, 1214, 1539, 1637, 2544, 2705, 2793
GluI GCNGC 2 cut(s) 927, 1084
GsaI CCCAGC 2 cut(s) 2061, 2427
HaeIII GGCC 3 cut(s) 463, 551, 2712
HapII CCGG 3 cut(s) 786, 2187, 2654
HincII GTYRAC 3 cut(s) 71, 514, 2136
HindII GTYRAC 3 cut(s) 71, 514, 2136
HindIII AAGCTT 2 cut(s) 311, 1649
HpaI GTTAAC 1 cut(s) 514
HpaII CCGG 3 cut(s) 786, 2187, 2654
HphI GGTGA 8 cut(s) 170, 419, 515, 704, 2221, 2291, 2326, 2408
Hpy188III TCNNGA 9 cut(s) 358, 398, 408, 1526, 2266, 2323, 2480, 2583, 2691
HpyCH4IV ACGT 5 cut(s) 1326, 2095, 2180, 2239, 2741
HpyF10VI GCNNNNNNNGC 5 cut(s) 319, 1496, 2032, 2317, 2499
HpySE526I ACGT 5 cut(s) 1326, 2095, 2180, 2239, 2741
KspAI GTTAAC 1 cut(s) 514
LguI GCTCTTC 4 cut(s) 261, 323, 1571, 2277
LmnI GCTCC 3 cut(s) 1495, 1816, 2893
Lsp1109I GCAGC 2 cut(s) 913, 1070
LweI GCATC 7 cut(s) 65, 160, 825, 2332, 2422, 2476, 2812
MaeI CTAG 8 cut(s) 530, 609, 1214, 1539, 1637, 2544, 2705, 2793
MaeII ACGT 5 cut(s) 1326, 2095, 2180, 2239, 2741
MaeIII GTNAC 5 cut(s) 710, 1122, 2159, 2396, 2533
MfeI CAATTG 1 cut(s) 2100
MflI RGATCY 2 cut(s) 788, 2677
MhlI GDGCHC 9 cut(s) 273, 372, 529, 1361, 1813, 2037, 2449, 2504, 2851
MlsI TGGCCA 1 cut(s) 551
MluNI TGGCCA 1 cut(s) 551
MlyI GAGTC 5 cut(s) 1928, 2312, 2472, 2655, 2777
MmeI TCCRAC 2 cut(s) 284, 2912
Mox20I TGGCCA 1 cut(s) 551
Mph1103I ATGCAT 2 cut(s) 1146, 2442
MroXI GAANNNNTTC 4 cut(s) 1456, 1777, 1996, 2221
MscI TGGCCA 1 cut(s) 551
MseI TTAA 6 cut(s) 41, 513, 752, 1503, 1662, 2664
Msp20I TGGCCA 1 cut(s) 551
MspA1I CMGCKG 3 cut(s) 206, 1943, 2927
MspCI CTTAAG 2 cut(s) 751, 1502
MspI CCGG 3 cut(s) 786, 2187, 2654
MspR9I CCNGG 3 cut(s) 2330, 2867, 2892
MunI CAATTG 1 cut(s) 2100
MvaI CCWGG 3 cut(s) 2330, 2867, 2892
MwoI GCNNNNNNNGC 5 cut(s) 319, 1496, 2032, 2317, 2499
NdeI CATATG 1 cut(s) 1519
NlaIV GGNNCC 5 cut(s) 202, 784, 904, 1322, 2327
NmeAIII GCCGAG 2 cut(s) 199, 348
NmuCI GTSAC 2 cut(s) 710, 2396
NsiI ATGCAT 2 cut(s) 1146, 2442
NspI RCATGY 3 cut(s) 236, 493, 1144
PaeI GCATGC 1 cut(s) 1144
PaqCI CACCTGC 1 cut(s) 2302
PceI AGGCCT 1 cut(s) 463
PciSI GCTCTTC 4 cut(s) 261, 323, 1571, 2277
PdmI GAANNNNTTC 4 cut(s) 1456, 1777, 1996, 2221
PflFI GACNNNGTC 1 cut(s) 2645
PflMI CCANNNNNTGG 1 cut(s) 959
PkrI GCNGC 2 cut(s) 928, 1085
PleI GAGTC 5 cut(s) 1927, 2311, 2471, 2654, 2777
PpsI GAGTC 5 cut(s) 1927, 2311, 2471, 2654, 2777
Ppu21I YACGTR 2 cut(s) 1327, 2096
PpuMI RGGWCCY 1 cut(s) 2771
Psp124BI GAGCTC 1 cut(s) 2037
Psp5II RGGWCCY 1 cut(s) 2771
Psp6I CCWGG 3 cut(s) 2328, 2865, 2890
PspFI CCCAGC 2 cut(s) 2057, 2423
PspGI CCWGG 3 cut(s) 2328, 2865, 2890
PspN4I GGNNCC 5 cut(s) 202, 784, 904, 1322, 2327
PspPI GGNCC 5 cut(s) 1321, 2710, 2771, 2840, 2894
PspPPI RGGWCCY 1 cut(s) 2771
PsrI GAACNNNNNNTAC 2 cut(s) 126, 158
PstNI CAGNNNCTG 4 cut(s) 926, 2626, 2639, 2774
PsuI RGATCY 2 cut(s) 788, 2677
PsyI GACNNNGTC 1 cut(s) 2645
PvuII CAGCTG 2 cut(s) 1943, 2927
RsaI GTAC 4 cut(s) 92, 1160, 1626, 2115
RsaNI GTAC 4 cut(s) 91, 1159, 1625, 2114
SacI GAGCTC 1 cut(s) 2037
SapI GCTCTTC 4 cut(s) 261, 323, 1571, 2277
SaqAI TTAA 6 cut(s) 41, 513, 752, 1503, 1662, 2664
SatI GCNGC 2 cut(s) 927, 1084
Sau96I GGNCC 5 cut(s) 1321, 2710, 2771, 2840, 2894
SchI GAGTC 5 cut(s) 1928, 2312, 2472, 2655, 2777
ScrFI CCNGG 3 cut(s) 2330, 2867, 2892
SduI GDGCHC 9 cut(s) 273, 372, 529, 1361, 1813, 2037, 2449, 2504, 2851
SfaNI GCATC 7 cut(s) 65, 160, 825, 2332, 2422, 2476, 2812
SfcI CTRYAG 5 cut(s) 1494, 1740, 1981, 2040, 2915
SinI GGWCC 4 cut(s) 1321, 2771, 2840, 2894
SmlI CTYRAG 3 cut(s) 751, 1031, 1502
SmoI CTYRAG 3 cut(s) 751, 1031, 1502
SpeI ACTAGT 1 cut(s) 1636
SphI GCATGC 1 cut(s) 1144
SseBI AGGCCT 1 cut(s) 463
SsiI CCGC 5 cut(s) 204, 533, 779, 2708, 2897
SspI AATATT 2 cut(s) 993, 1456
SspMI CTAG 8 cut(s) 530, 609, 1214, 1539, 1637, 2544, 2705, 2793
SstI GAGCTC 1 cut(s) 2037
StuI AGGCCT 1 cut(s) 463
StyD4I CCNGG 3 cut(s) 2328, 2865, 2890
StyI CCWWGG 2 cut(s) 1461, 2543
TaiI ACGT 5 cut(s) 1329, 2098, 2183, 2242, 2744
TaqI TCGA 8 cut(s) 260, 353, 633, 678, 964, 1970, 2461, 2937
TatI WGTACW 1 cut(s) 1624
Tru1I TTAA 6 cut(s) 41, 513, 752, 1503, 1662, 2664
Tru9I TTAA 6 cut(s) 41, 513, 752, 1503, 1662, 2664
TscAI CASTG 6 cut(s) 349, 377, 884, 2044, 2232, 2280
TseFI GTSAC 2 cut(s) 710, 2396
TseI GCWGC 2 cut(s) 926, 1083
Tsp45I GTSAC 2 cut(s) 710, 2396
TspDTI ATGAA 7 cut(s) 119, 129, 590, 647, 752, 1064, 2624
TspGWI ACGGA 1 cut(s) 1443
TspRI CASTG 6 cut(s) 349, 377, 884, 2044, 2232, 2280
Tth111I GACNNNGTC 1 cut(s) 2645
Van91I CCANNNNNTGG 1 cut(s) 959
Vha464I CTTAAG 2 cut(s) 751, 1502
VneI GTGCAC 2 cut(s) 368, 2500
VpaK11BI GGWCC 4 cut(s) 1321, 2771, 2840, 2894
XapI RAATTY 9 cut(s) 22, 220, 841, 1301, 1331, 1415, 2475, 2716, 2920
XceI RCATGY 3 cut(s) 236, 493, 1144
XmaJI CCTAGG 1 cut(s) 2543
XmiI GTMKAC 3 cut(s) 1264, 1425, 1743
XmnI GAANNNNTTC 4 cut(s) 1456, 1777, 1996, 2221
XspI CTAG 8 cut(s) 530, 609, 1214, 1539, 1637, 2544, 2705, 2793
Zsp2I ATGCAT 2 cut(s) 1146, 2442
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.