RLG00000034097

metal ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000343
Physical Location & Seq
Reverse (-)
36720126 .. 36734051
13926 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034097

Sequence Viewer

Length: 2496 bp
ATGATCTTAGTCTTCTTGGCCACTGGAATAACCAAGTTCATGAGACTTGAGGACGGTTGCAATGTGGTGACAAAAAAGAGCATGCAAGGGAAATGGTTGAATTGTCACTCAACAAAAGATGCTAGGGTGGAAAGGCTTTTGTTTCTAAGTTTAATTACCGGCTCCCCTTCGCCGTTCATCGATCAACAATATACCAAGCTTTCAGGGGTGAAAGAAGTGTTTGAATTGAAGGAGGAGATGGAATCATCAGATGATGAGGCTGAGGCAATGCAGCTTTGGGTTTCAAATTACTACTTTGTAGATGGCAAGGAAGAGCCTATCTCCTTTCATGTGCTGCCAATTCAATGGAGGGATGGTGATAGGCAGGATGGGAAGGAAGCAGTGTTGTTTCTGCATGGCAGTGCCGACAATGGGCTTGAGAAGATTTACAAACCTGTTGTTGCTTGGAAGTTTGATCTTTCGAATGTGAAGCCCGAGATTGCAGTGCTTTCCAAGGAGAATGATTGGATTCTGCTCCAGAAGCCGAGGAGGAGCTATGAGGGGATCGTAAGGACTGTCTTGATAACTGTGCAGTGCCTTAGTTATGCAGTGTGGAATGCTGAGGTGTCCGGAAAAGCTGTGTGGGATCACTTGTCTAAAGCGTTCAGCATGTATGAGAACAGGCCTTCGCAGCATGATTTGGGTGATCACAGGAGTCTGATTAGTGAAGCTCTAAGGCGAGATGATGCCTTGGCAAAGTCGAAGAGTAGGCTTTCAATCAATTATCAAAACTGGAGTGGCCACAGGACGACTGTCGCCTATTCACAGAGAATCCTATACAGCAAGCTTAGAGTATATCTTGCACCTTTCTTGCATGGAAAGCGGTACACGTCCTTCGGTCGCCATTTCACAAAGGTGGAAAAACTCGAACAGATTGCTGATAGGCTCCATTCATACGTACAGAATGGAGACACGATAGTGGACTTCTGTTGTGGCTCTAATGATTTCAGCATCGTAATGAAGAAAAAGCTTGAGGAGACAGGGAAGAAGTGCCTTTTTAGAAATTATGACATCATTCAACCAAAGAATGATTTCTGTTTTGAGAAGAGAGATTGGATGACTGTTCAAAAAGGTGAATTACCGGATAGTAGTAAATTGATCATGGGGTTGAACCCTCCTTTTGGAGTTAAAGCATCTTTGGCAAACCAGTTTATTGATAAGGCTCTGCAGTTCCAGCCGAAGCTCCTTATTCTTATTGTTCCACCAGAAACAAAAGGGTTACAATGTTATGATCTGATATGGAAGGATGAACGGTCTCTGTCAGGAAAGTCCTTTTATCTTCCTGGATCTGTTGATGAGAATGACAAGCAAATGGATCAGTGGAATGTGACGACACCTCCACTCTATCTTTGGAGTCGTCCTGATTGGTCTGAGAGACACAGAGCTATTACTCAAAAGGATAGTCACGGTTCCATGTCACAAGGGTCAGTGGAAGATGGTCATTCAGTTGAGAATAAGGATGAAAATGTTGAAAATCTCATGTTAAAAAATGCTTATCTGACACCGACCGGTAATTCCAGAGATTTTGTTGGTGGGTCAGTTGCAGGCGAGGGACATGAAGATAGATCCTCCAAGGGCAATGATGACAGAGGTTACCATGCGAGTTCTGGTCACCAGAAGAACCAGGTTAGTAAGATTTCTGAGAGGCCGCAGTGTGGTGGCAGAAAAAAGAATGGGAGTGGAGTGGTTGAGTTGTCGCCAGACAACAAATGTGATGGTGGCAATGTTTCTAGTGAGATTCAGATGGAAAGGTCTCCATCTACTGAGCGACAGCGGAAAGCAAATCAGCAGTCTGAGCCCAGCGATTCTGGTTCAAGTATGCATTTCAGAACTGCTTGTGGGAGGACCACAGTCAGCATCCCTGATGACACTGGCAGCAATGTCACAAGCAGTGCTGACCCTTATTCAAGTGTGACTCATAGATGGTCCAGTGGTGCCAGTCCTAGCTCTATATATAGGGATACAAAATTGGAGGAACCTGTTTTGGGTTACATGAGAGAGACAGATAACTTTGGTTATAGACCGTATTCCATGGGGATGGAAGACCCATATAGGAGAGACTCCGATATACGCGCACAAGTTCGCCATTACGGGCAGCAAGATTCTGATATGTTCAGAAGTAATTATCTAGCAGGTCAGGATTTGTTACGTGGTCGGGTTGGATCATTTTCGTCCACGTATGGCCATGCTCATCTAGGTTCAGCAGTTGACTCATCTTACAGAATGAACATGTCAGCCATGCAGCGATATGAACCTCGGCTGGATGAATTGAACCACACAAGAGCGAGTAACTTAGGAACCCAACCAGCGTTTGGGTATGATCCATGCACATACAGTAGCAACATGTATGATCCCAGGGCACCCCGACCTGGTGACCAAGGGGCCCCCTTTGGTTTTGCTCCCGGTTCTCACAGAAGCTTCTCAAACCGGAATTCAGCAGGTTGGCTGAACGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006325 GO:0006355 GO:0006950 GO:0006952 GO:0006996 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0009605 GO:0009607 GO:0009620 GO:0009791 GO:0009889 GO:0009893 GO:0009909 GO:0009911 GO:0009987 GO:0010228 GO:0010468 GO:0010556 GO:0010604 GO:0010638 GO:0016043 GO:0019219 GO:0019222 GO:0022414 GO:0023052 GO:0030154 GO:0031056 GO:0031058 GO:0031060 GO:0031062 GO:0031323 GO:0031325 GO:0031326 GO:0031399 GO:0031401 GO:0031440 GO:0031935 GO:0031937 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033043 GO:0033044 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0043954 GO:0044030 GO:0044424 GO:0044464 GO:0048366 GO:0048367 GO:0048468 GO:0048518 GO:0048522 GO:0048580 GO:0048582 GO:0048608 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0048869 GO:0050684 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051052 GO:0051094 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051276 GO:0051570 GO:0051574 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060968 GO:0061085 GO:0061087 GO:0061458 GO:0065007 GO:0070827 GO:0070828 GO:0070829 GO:0071840 GO:0080090 GO:0090436 GO:0097159 GO:0098542 GO:0099402 GO:0140110 GO:1900109 GO:1900111 GO:1900363 GO:1901363 GO:1902275 GO:1903311 GO:1903506 GO:1905269 GO:2000024 GO:2000026 GO:2000112 GO:2000241 GO:2000243 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNMT1-RFD

Protein Analysis

832

Amino Acids

93.96

Weight (kDa)

7.09

Isoelectric Point (pI)

41.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNMT1-RFD PF12047 94 - 190 1.4e-06 Cytosine specific DNA methyltransferase replication foci domain
Mtase_EDM2 PF26055 312 - 478 8.6e-88 EDM2 methyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000343)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48090 AT5G48090 AT5G48090 AT5G55390 AT5G55390 AT5G55390
fragaria_vesca FvH4_3g23690 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160
malus_domestica MD03G1191000.v1.1 MD11G1207800.v1.1 MD11G1208000.v1.1
prunus_persica Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227600_v2.0.a1 Prupe.4G227700_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1
pyrus_communis pycom01g03400 pycom03g14420 pycom11g18100 pycom11g18140 pycom11g18150 pycom11g18160
rosa_chinensis RchiOBHm_Chr2g0145981 RchiOBHm_Chr4g0405241 RchiOBHm_Chr4g0405271 RchiOBHm_Chr5g0016201 RchiOBHm_Chr5g0041831 RchiOBHm_Chr5g0041871 RchiOBHm_Chr5g0041881 RchiOBHm_Chr5g0059241
rosa_laevigata RLG00000002117 RLG00000008861 RLG00000008862 RLG00000011000 RLG00000033216 RLG00000034097 RLG00000034101
rosa_multiflora Rmu_sc0000161.1_g000001 Rmu_sc0000861.1_g000040 Rmu_sc0007522.1_g000003 Rmu_sc0009443.1_g000015 Rmu_sc0022129.1_g000001 Rmu_sc0042315.1_g000001
rosa_roxburghii Rroxscaffold_1G00038660 Rroxscaffold_1G00038690 Rroxscaffold_5G00349560 Rroxscaffold_5G00349610 Rroxscaffold_6G00388370 Rroxscaffold_7G00188740
rosa_rugosa Rorug03G0199700 Rorug04G0055400 Rorug04G0055500 Rorug04G0055900 Rorug04G0056000 Rorug04G0056100 Rorug04G0056100 Rorug04G0056200 Rorug05G0195600 Rorug05G0195700 Rorug05G0195800 Rorug05G0195900 Rorug05G0196800 Rorug05G0385000
rosa_samantha Rh4AG129900 Rh4AG130100 Rh4BG124200 Rh4BG124400 Rh4BG124500 Rh4CG137200 Rh4CG137300 Rh4DG123500 Rh4DG123700 Rh5AG280800 Rh5AG281400 Rh5BG286700 Rh5BG287000 Rh5BG397800 Rh5CG318100 Rh5CG318600 Rh5CG421600 Rh5DG132300 Rh5DG132400 Rh5DG295400 Rh5DG295700 Rh7BG475300
rosa_wichuraiana Rw0G001330 Rw0G005730 Rw4G008670 Rw4G010510 Rw4G010540 Rw5G026450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 2162, 2468
AccB1I GGYRCC 2 cut(s) 1973, 2398
AccB7I CCANNNNNTGG 1 cut(s) 2351
AccII CGCG 1 cut(s) 2112
AccIII TCCGGA 1 cut(s) 608
AciI CCGC 3 cut(s) 862, 1688, 1813
AclWI GGATC 8 cut(s) 551, 633, 1333, 1362, 1599, 2209, 2354, 2384
AcoI YGGCCR 3 cut(s) 18, 778, 2221
AcsI RAATTY 1 cut(s) 2470
AfaI GTAC 2 cut(s) 866, 939
AfiI CCNNNNNNNGG 6 cut(s) 411, 1160, 1694, 2024, 2351, 2408
AflIII ACRYGT 3 cut(s) 867, 2268, 2382
AgeI ACCGGT 1 cut(s) 1547
AjiI CACGTC 1 cut(s) 870
AjnI CCWGG 4 cut(s) 1321, 1662, 2393, 2407
AleI CACNNNNGTG 2 cut(s) 893, 956
Alw26I GTCTC 8 cut(s) 37, 942, 1010, 1299, 1408, 1797, 2033, 2091
AlwI GGATC 8 cut(s) 551, 633, 1333, 1362, 1599, 2209, 2354, 2384
Ama87I CYCGRG 1 cut(s) 473
Aor13HI TCCGGA 1 cut(s) 608
AoxI GGCC 6 cut(s) 18, 662, 778, 1685, 2221, 2421
ApaI GGGCCC 1 cut(s) 2425
ApeKI GCWGC 6 cut(s) 271, 334, 670, 1914, 2134, 2281
ApoI RAATTY 1 cut(s) 2470
ArsI GACNNNNNNTTYG 4 cut(s) 1426, 1458, 1814, 1846
AsiGI ACCGGT 1 cut(s) 1547
Asp700I GAANNNNTTC 1 cut(s) 1070
AspLEI GCGC 1 cut(s) 2114
AspS9I GGNCC 4 cut(s) 1884, 1965, 2421, 2422
AsuC2I CCSGG 1 cut(s) 2442
AsuHPI GGTGA 7 cut(s) 79, 220, 368, 695, 1124, 1643, 2423
AsuII TTCGAA 1 cut(s) 461
AvaI CYCGRG 1 cut(s) 473
AvaII GGWCC 2 cut(s) 1884, 1965
BaeGI GKGCMC 2 cut(s) 2401, 2425
BalI TGGCCA 3 cut(s) 20, 780, 2223
BanI GGYRCC 2 cut(s) 1973, 2398
BanII GRGCYC 2 cut(s) 1839, 2425
BarI GAAGNNNNNNTAC 2 cut(s) 857, 889
BbsI GAAGAC 2 cut(s) 4, 2088
BbvCI CCTCAGC 2 cut(s) 261, 600
BbvI GCAGC 6 cut(s) 283, 321, 682, 1926, 2146, 2293
BceAI ACGGC 1 cut(s) 157
BcgI CGANNNNNNTGC 2 cut(s) 896, 930
BciT130I CCWGG 4 cut(s) 1323, 1664, 2395, 2409
BciVI GTATCC 1 cut(s) 1993
BclI TGATCA 2 cut(s) 685, 1137
BcnI CCSGG 1 cut(s) 2442
BcoDI GTCTC 8 cut(s) 37, 942, 1010, 1299, 1408, 1797, 2033, 2091
BfaI CTAG 5 cut(s) 123, 1770, 1983, 2168, 2234
BfmI CTRYAG 1 cut(s) 1205
BfuAI ACCTGC 2 cut(s) 2162, 2468
BfuI GTATCC 1 cut(s) 1993
BisI GCNGC 7 cut(s) 272, 335, 671, 1688, 1915, 2135, 2282
BlsI GCNGC 7 cut(s) 273, 336, 672, 1689, 1916, 2136, 2283
Bme1390I CCNGG 5 cut(s) 1323, 1664, 2395, 2409, 2442
Bme18I GGWCC 2 cut(s) 1884, 1965
BmeT110I CYCGRG 1 cut(s) 473
BmgBI CACGTC 1 cut(s) 870
BmgT120I GGNCC 4 cut(s) 1884, 1965, 2421, 2422
BmrFI CCNGG 5 cut(s) 1323, 1664, 2395, 2409, 2442
BmsI GCATC 5 cut(s) 109, 715, 999, 1181, 1905
BoxI GACNNNNGTC 2 cut(s) 791, 1889
BpiI GAAGAC 2 cut(s) 4, 2088
BplI GAGNNNNNCTC 2 cut(s) 305, 337
BpmI CTGGAG 2 cut(s) 500, 793
Bpu10I CCTNAGC 2 cut(s) 261, 600
Bpu14I TTCGAA 1 cut(s) 461
BpuEI CTTGAG 3 cut(s) 68, 437, 1031
BpuMI CCSGG 1 cut(s) 2442
Bsa29I ATCGAT 1 cut(s) 180
BsaAI YACGTR 3 cut(s) 937, 2189, 2217
BsaI GGTCTC 2 cut(s) 1299, 1797
BsaJI CCNNGG 9 cut(s) 492, 524, 729, 1611, 2070, 2294, 2393, 2394, 2416
BsaWI WCCGGW 4 cut(s) 608, 1120, 1547, 2466
BsaXI ACNNNNNCTCC 4 cut(s) 340, 370, 1360, 1390
Bsc4I CCNNNNNNNGG 6 cut(s) 411, 1160, 1694, 2024, 2351, 2408
Bse118I RCCGGY 2 cut(s) 158, 1547
Bse1I ACTGG 6 cut(s) 28, 776, 1186, 1915, 1968, 1977
Bse3DI GCAATG 5 cut(s) 67, 273, 1624, 1768, 1924
BseAI TCCGGA 1 cut(s) 608
BseBI CCWGG 4 cut(s) 1323, 1664, 2395, 2409
BseCI ATCGAT 1 cut(s) 180
BseDI CCNNGG 9 cut(s) 492, 524, 729, 1611, 2070, 2294, 2393, 2394, 2416
BseGI GGATG 8 cut(s) 358, 373, 1101, 1291, 1504, 1896, 2082, 2308
BseLI CCNNNNNNNGG 6 cut(s) 411, 1160, 1694, 2024, 2351, 2408
BseMI GCAATG 5 cut(s) 67, 273, 1624, 1768, 1924
BseMII CTCAG 6 cut(s) 252, 591, 1401, 1671, 1794, 1824
BseNI ACTGG 6 cut(s) 28, 776, 1186, 1915, 1968, 1977
BseRI GAGGAG 4 cut(s) 248, 541, 544, 1028
BseSI GKGCMC 2 cut(s) 2401, 2425
BseXI GCAGC 6 cut(s) 283, 321, 682, 1926, 2146, 2293
BseYI CCCAGC 1 cut(s) 1838
BsgI GTGCAG 1 cut(s) 590
Bsh1236I CGCG 1 cut(s) 2112
Bsh1285I CGRYCG 2 cut(s) 880, 1548
BshFI GGCC 6 cut(s) 20, 664, 780, 1687, 2223, 2423
BshNI GGYRCC 2 cut(s) 1973, 2398
BshTI ACCGGT 1 cut(s) 1547
BshVI ATCGAT 1 cut(s) 180
BsiEI CGRYCG 2 cut(s) 880, 1548
BsiHKCI CYCGRG 1 cut(s) 473
BsiSI CCGG 6 cut(s) 159, 609, 1121, 1548, 2442, 2467
BslFI GGGAC 1 cut(s) 1605
BslI CCNNNNNNNGG 6 cut(s) 411, 1160, 1694, 2024, 2351, 2408
BsmAI GTCTC 8 cut(s) 37, 942, 1010, 1299, 1408, 1797, 2033, 2091
BsmFI GGGAC 1 cut(s) 1605
BsmI GAATGC 1 cut(s) 601
BsnI GGCC 6 cut(s) 20, 664, 780, 1687, 2223, 2423
Bso31I GGTCTC 2 cut(s) 1299, 1797
BsoBI CYCGRG 1 cut(s) 473
Bsp119I TTCGAA 1 cut(s) 461
Bsp120I GGGCCC 1 cut(s) 2421
Bsp1286I GDGCHC 3 cut(s) 1839, 2401, 2425
Bsp13I TCCGGA 1 cut(s) 608
Bsp19I CCATGG 1 cut(s) 2070
BspACI CCGC 3 cut(s) 862, 1688, 1813
BspANI GGCC 6 cut(s) 20, 664, 780, 1687, 2223, 2423
BspCNI CTCAG 6 cut(s) 253, 592, 1402, 1672, 1795, 1825
BspDI ATCGAT 1 cut(s) 180
BspEI TCCGGA 1 cut(s) 608
BspFNI CGCG 1 cut(s) 2112
BspHI TCATGA 1 cut(s) 39
BspMAI CTGCAG 1 cut(s) 1209
BspMI ACCTGC 2 cut(s) 2162, 2468
BspPI GGATC 8 cut(s) 551, 633, 1333, 1362, 1599, 2209, 2354, 2384
BspQI GCTCTTC 1 cut(s) 306
BspT104I TTCGAA 1 cut(s) 461
BspT107I GGYRCC 2 cut(s) 1973, 2398
BspTNI GGTCTC 2 cut(s) 1299, 1797
BsrDI GCAATG 5 cut(s) 67, 273, 1624, 1768, 1924
BsrFI RCCGGY 2 cut(s) 158, 1547
BsrI ACTGG 6 cut(s) 28, 776, 1186, 1915, 1968, 1977
BssAI RCCGGY 2 cut(s) 158, 1547
BssECI CCNNGG 9 cut(s) 492, 524, 729, 1611, 2070, 2294, 2393, 2394, 2416
BssT1I CCWWGG 5 cut(s) 492, 729, 1611, 2070, 2416
Bst2UI CCWGG 4 cut(s) 1323, 1664, 2395, 2409
Bst6I CTCTTC 3 cut(s) 306, 737, 1079
BstBAI YACGTR 3 cut(s) 937, 2189, 2217
BstBI TTCGAA 1 cut(s) 461
BstC8I GCNNGC 3 cut(s) 83, 824, 1585
BstDSI CCRYGG 1 cut(s) 2070
BstEII GGTNACC 3 cut(s) 1631, 1649, 2411
BstF5I GGATG 8 cut(s) 358, 373, 1101, 1291, 1504, 1896, 2082, 2308
BstFNI CGCG 1 cut(s) 2112
BstHHI GCGC 1 cut(s) 2114
BstMAI GTCTC 8 cut(s) 37, 942, 1010, 1299, 1408, 1797, 2033, 2091
BstMCI CGRYCG 2 cut(s) 880, 1548
BstMWI GCNNNNNNNGC 6 cut(s) 520, 670, 859, 1178, 1213, 1834
BstNI CCWGG 4 cut(s) 1323, 1664, 2395, 2409
BstNSI RCATGY 4 cut(s) 85, 652, 2272, 2386
BstPAI GACNNNNGTC 2 cut(s) 791, 1889
BstPI GGTNACC 3 cut(s) 1631, 1649, 2411
BstSCI CCNGG 5 cut(s) 1321, 1662, 2393, 2407, 2440
BstSFI CTRYAG 1 cut(s) 1205
BstSLI GKGCMC 2 cut(s) 2401, 2425
BstSNI TACGTA 1 cut(s) 937
BstUI CGCG 1 cut(s) 2112
BstV1I GCAGC 6 cut(s) 283, 321, 682, 1926, 2146, 2293
BstV2I GAAGAC 2 cut(s) 4, 2088
BstX2I RGATCY 2 cut(s) 1325, 1604
BstXI CCANNNNNNTGG 2 cut(s) 345, 2077
BstYI RGATCY 2 cut(s) 1325, 1604
Bsu15I ATCGAT 1 cut(s) 180
BsuI GTATCC 1 cut(s) 1993
BsuRI GGCC 6 cut(s) 20, 664, 780, 1687, 2223, 2423
BsuTUI ATCGAT 1 cut(s) 180
BtgI CCRYGG 1 cut(s) 2070
BtrI CACGTC 1 cut(s) 870
BtsCI GGATG 8 cut(s) 358, 373, 1101, 1291, 1504, 1896, 2082, 2308
BtsI GCAGTG 7 cut(s) 387, 406, 489, 578, 594, 1697, 1936
BveI ACCTGC 2 cut(s) 2162, 2468
Cac8I GCNNGC 3 cut(s) 83, 824, 1585
CciI TCATGA 1 cut(s) 39
CfoI GCGC 1 cut(s) 2114
Cfr10I RCCGGY 2 cut(s) 158, 1547
Cfr13I GGNCC 4 cut(s) 1884, 1965, 2421, 2422
ClaI ATCGAT 1 cut(s) 180
CsiI ACCWGGT 2 cut(s) 1662, 2407
Csp6I GTAC 2 cut(s) 865, 938
CspAI ACCGGT 1 cut(s) 1547
CviQI GTAC 2 cut(s) 865, 938
EaeI YGGCCR 3 cut(s) 18, 778, 2221
Eam1104I CTCTTC 3 cut(s) 306, 737, 1079
EarI CTCTTC 3 cut(s) 306, 737, 1079
Eco105I TACGTA 1 cut(s) 937
Eco130I CCWWGG 5 cut(s) 492, 729, 1611, 2070, 2416
Eco147I AGGCCT 1 cut(s) 664
Eco24I GRGCYC 2 cut(s) 1839, 2425
Eco31I GGTCTC 2 cut(s) 1299, 1797
Eco47I GGWCC 2 cut(s) 1884, 1965
Eco88I CYCGRG 1 cut(s) 473
Eco91I GGTNACC 3 cut(s) 1631, 1649, 2411
EcoO109I RGGNCCY 2 cut(s) 2421, 2422
EcoO65I GGTNACC 3 cut(s) 1631, 1649, 2411
EcoRI GAATTC 1 cut(s) 2470
EcoRII CCWGG 4 cut(s) 1321, 1662, 2393, 2407
EcoT14I CCWWGG 5 cut(s) 492, 729, 1611, 2070, 2416
EcoT22I ATGCAT 1 cut(s) 1863
EcoT38I GRGCYC 2 cut(s) 1839, 2425
ErhI CCWWGG 5 cut(s) 492, 729, 1611, 2070, 2416
FaqI GGGAC 1 cut(s) 1605
FbaI TGATCA 2 cut(s) 685, 1137
Fnu4HI GCNGC 7 cut(s) 272, 335, 671, 1688, 1915, 2135, 2282
FokI GGATG 8 cut(s) 365, 380, 1108, 1298, 1511, 1883, 2089, 2315
FriOI GRGCYC 2 cut(s) 1839, 2425
Fsp4HI GCNGC 7 cut(s) 272, 335, 671, 1688, 1915, 2135, 2282
FspBI CTAG 5 cut(s) 123, 1770, 1983, 2168, 2234
GlaI GCGC 1 cut(s) 2113
GluI GCNGC 7 cut(s) 272, 335, 671, 1688, 1915, 2135, 2282
GsaI CCCAGC 1 cut(s) 1842
GsuI CTGGAG 2 cut(s) 500, 793
HaeIII GGCC 6 cut(s) 20, 664, 780, 1687, 2223, 2423
HapII CCGG 6 cut(s) 159, 609, 1121, 1548, 2442, 2467
HhaI GCGC 1 cut(s) 2114
Hin6I GCGC 1 cut(s) 2112
HinP1I GCGC 1 cut(s) 2112
HincII GTYRAC 1 cut(s) 2248
HindII GTYRAC 1 cut(s) 2248
HindIII AAGCTT 4 cut(s) 197, 824, 1007, 2455
HpaII CCGG 6 cut(s) 159, 609, 1121, 1548, 2442, 2467
HphI GGTGA 7 cut(s) 79, 220, 368, 695, 1124, 1643, 2423
Hpy166II GTNNAC 4 cut(s) 867, 961, 2214, 2248
Hpy188III TCNNGA 8 cut(s) 40, 517, 559, 609, 1302, 1400, 1557, 2177
Hpy8I GTNNAC 4 cut(s) 867, 961, 2214, 2248
HpyAV CCTTC 6 cut(s) 177, 223, 367, 675, 883, 1276
HpyCH4IV ACGT 4 cut(s) 869, 936, 2188, 2216
HpyF10VI GCNNNNNNNGC 6 cut(s) 520, 670, 859, 1178, 1213, 1834
HpySE526I ACGT 4 cut(s) 869, 936, 2188, 2216
HspAI GCGC 1 cut(s) 2112
Kpn2I TCCGGA 1 cut(s) 608
Ksp22I TGATCA 2 cut(s) 685, 1137
LguI GCTCTTC 1 cut(s) 306
LmnI GCTCC 6 cut(s) 167, 519, 531, 930, 1227, 2443
Lsp1109I GCAGC 6 cut(s) 283, 321, 682, 1926, 2146, 2293
LweI GCATC 5 cut(s) 109, 715, 999, 1181, 1905
MabI ACCWGGT 2 cut(s) 1662, 2407
MaeI CTAG 5 cut(s) 123, 1770, 1983, 2168, 2234
MaeII ACGT 4 cut(s) 869, 936, 2188, 2216
MflI RGATCY 2 cut(s) 1325, 1604
MhlI GDGCHC 3 cut(s) 1839, 2401, 2425
MlsI TGGCCA 3 cut(s) 20, 780, 2223
MluNI TGGCCA 3 cut(s) 20, 780, 2223
MlyI GAGTC 5 cut(s) 703, 1402, 1948, 2093, 2243
MmeI TCCRAC 1 cut(s) 2179
Mox20I TGGCCA 3 cut(s) 20, 780, 2223
Mph1103I ATGCAT 1 cut(s) 1863
MroI TCCGGA 1 cut(s) 608
MroXI GAANNNNTTC 1 cut(s) 1070
MscI TGGCCA 3 cut(s) 20, 780, 2223
MseI TTAA 3 cut(s) 152, 1167, 1523
MslI CAYNNNNRTG 5 cut(s) 399, 893, 956, 995, 2075
Msp20I TGGCCA 3 cut(s) 20, 780, 2223
MspA1I CMGCKG 1 cut(s) 1813
MspI CCGG 6 cut(s) 159, 609, 1121, 1548, 2442, 2467
MspR9I CCNGG 5 cut(s) 1323, 1664, 2395, 2409, 2442
Mva1269I GAATGC 1 cut(s) 601
MvaI CCWGG 4 cut(s) 1323, 1664, 2395, 2409
MvnI CGCG 1 cut(s) 2112
MwoI GCNNNNNNNGC 6 cut(s) 520, 670, 859, 1178, 1213, 1834
NciI CCSGG 1 cut(s) 2442
NcoI CCATGG 1 cut(s) 2070
NmeAIII GCCGAG 2 cut(s) 549, 2275
NmuCI GTSAC 9 cut(s) 67, 104, 1366, 1442, 1455, 1649, 1921, 1951, 2411
NsiI ATGCAT 1 cut(s) 1863
NspI RCATGY 4 cut(s) 85, 652, 2272, 2386
NspV TTCGAA 1 cut(s) 461
OliI CACNNNNGTG 2 cut(s) 893, 956
PaeI GCATGC 1 cut(s) 85
PagI TCATGA 1 cut(s) 39
PasI CCCWGGG 1 cut(s) 2394
PceI AGGCCT 1 cut(s) 664
PciI ACATGT 2 cut(s) 2268, 2382
PciSI GCTCTTC 1 cut(s) 306
PctI GAATGC 1 cut(s) 601
PdmI GAANNNNTTC 1 cut(s) 1070
PfeI GAWTC 6 cut(s) 242, 508, 810, 1777, 1844, 2141
PflMI CCANNNNNTGG 1 cut(s) 2351
PfoI TCCNGGA 1 cut(s) 1321
PinAI ACCGGT 1 cut(s) 1547
PkrI GCNGC 7 cut(s) 273, 336, 672, 1689, 1916, 2136, 2283
PleI GAGTC 5 cut(s) 702, 1401, 1948, 2093, 2243
PpsI GAGTC 5 cut(s) 702, 1401, 1948, 2093, 2243
Ppu21I YACGTR 3 cut(s) 937, 2189, 2217
PscI ACATGT 2 cut(s) 2268, 2382
PshAI GACNNNNGTC 2 cut(s) 791, 1889
Psp6I CCWGG 4 cut(s) 1321, 1662, 2393, 2407
PspEI GGTNACC 3 cut(s) 1631, 1649, 2411
PspFI CCCAGC 1 cut(s) 1838
PspGI CCWGG 4 cut(s) 1321, 1662, 2393, 2407
PspOMI GGGCCC 1 cut(s) 2421
PspPI GGNCC 4 cut(s) 1884, 1965, 2421, 2422
PstI CTGCAG 1 cut(s) 1209
PsuI RGATCY 2 cut(s) 1325, 1604
RsaI GTAC 2 cut(s) 866, 939
RsaNI GTAC 2 cut(s) 865, 938
RseI CAYNNNNRTG 5 cut(s) 399, 893, 956, 995, 2075
SapI GCTCTTC 1 cut(s) 306
SaqAI TTAA 3 cut(s) 152, 1167, 1523
SatI GCNGC 7 cut(s) 272, 335, 671, 1688, 1915, 2135, 2282
Sau96I GGNCC 4 cut(s) 1884, 1965, 2421, 2422
SchI GAGTC 5 cut(s) 703, 1402, 1948, 2093, 2243
ScrFI CCNGG 5 cut(s) 1323, 1664, 2395, 2409, 2442
SduI GDGCHC 3 cut(s) 1839, 2401, 2425
SexAI ACCWGGT 2 cut(s) 1662, 2407
SfaNI GCATC 5 cut(s) 109, 715, 999, 1181, 1905
SfcI CTRYAG 1 cut(s) 1205
SfuI TTCGAA 1 cut(s) 461
SinI GGWCC 2 cut(s) 1884, 1965
SmiMI CAYNNNNRTG 5 cut(s) 399, 893, 956, 995, 2075
SmlI CTYRAG 3 cut(s) 47, 416, 1010
SmoI CTYRAG 3 cut(s) 47, 416, 1010
SnaBI TACGTA 1 cut(s) 937
SphI GCATGC 1 cut(s) 85
SseBI AGGCCT 1 cut(s) 664
SsiI CCGC 3 cut(s) 862, 1688, 1813
SspMI CTAG 5 cut(s) 123, 1770, 1983, 2168, 2234
StuI AGGCCT 1 cut(s) 664
StyD4I CCNGG 5 cut(s) 1321, 1662, 2393, 2407, 2440
StyI CCWWGG 5 cut(s) 492, 729, 1611, 2070, 2416
TaiI ACGT 4 cut(s) 872, 939, 2191, 2219
TaqI TCGA 4 cut(s) 180, 461, 740, 906
TaqII GACCGA 1 cut(s) 866
TauI GCSGC 1 cut(s) 1690
TfiI GAWTC 6 cut(s) 242, 508, 810, 1777, 1844, 2141
Tru1I TTAA 3 cut(s) 152, 1167, 1523
Tru9I TTAA 3 cut(s) 152, 1167, 1523
TseFI GTSAC 9 cut(s) 67, 104, 1366, 1442, 1455, 1649, 1921, 1951, 2411
TseI GCWGC 6 cut(s) 271, 334, 670, 1914, 2134, 2281
Tsp45I GTSAC 9 cut(s) 67, 104, 1366, 1442, 1455, 1649, 1921, 1951, 2411
Van91I CCANNNNNTGG 1 cut(s) 2351
VpaK11BI GGWCC 2 cut(s) 1884, 1965
XapI RAATTY 1 cut(s) 2470
XceI RCATGY 4 cut(s) 85, 652, 2272, 2386
XcmI CCANNNNNNNNNTGG 3 cut(s) 1386, 1643, 2348
XmnI GAANNNNTTC 1 cut(s) 1070
XspI CTAG 5 cut(s) 123, 1770, 1983, 2168, 2234
Zsp2I ATGCAT 1 cut(s) 1863
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.