Rroxscaffold_1G00038690

metal ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000343
Physical Location & Seq
Forward (+)
56220745 .. 56232127
11383 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00038690.1

Sequence Viewer

Length: 3738 bp
ATGGAATCGTCAGATGATGAGGCTGAGGCAATGCAGCTTTCGGTTTCGAATTACTACTTCGTAGATGGCAAGGAAGAGCCTATCTCTTTTCATGTGTTGCCAATTCAATGGAGTGAGGGTGATAGGCAGGATGGGAAGGAAGCAGTGTTGTTTCTGCATGGCAGTGCTGACAATGGGCTCGAGAAGATTTACAAACCTGTTGTTGCTTGGAAGTTTGATCTTTCGAATGTGAAGCCCGAGATTGCAGTGCTTTCCAAGGAGAATGATTGGATTCTGCTCCAGAAGCCGAGGAGGAGCTATGAGGGGATCGTAAGGACTGTCTTGATAACTGTGCAGTGCCTTAGTTATGCAATGTGGAATGCTGAGGCGTCTGGAAAAGCTGTGTGGGATCACTTGTCTAAAGCGTTCAGCATGTATGAGAACAGGCCTTCGCAGCATGATTTGGGTGATCACAGGGCTCTGATTAGTGAAGCTCTAAAGCGAGATGATGCCTTGGCAAAGTCGAAGTTTATAATTGACTTTCTTAAAGAAGAGCCACCCACAAAGAGGAAACTATCTGATGAGGATATTCAAGCTACAGCAAAGCTTGGATTTATAGTTGATGATGCGGAAGATTATATAATTGATGATGAAGATGAGTCAGATGATGATGATGATGATAACCTGTTTGATTCTGTTTGTGCCTTTTGTGATAATGGTGGCAAGCTGTTATGTTGTGAAGGGAGATGCCTAAGGTCCTTCCATCCCACGGTAGAGGACGGCGAGGACTCAATGTGTGAATCTCTTGGATTCACTCGGGAGGAAGTAGATGCAATGCCAAGTTTCTTCTGCAAGAACTGTCAATGTAAGCAGCATCAGTGCTTTGCTTGTGGGAAGTTAGGCTCCTCAGACAAATCTTTGGGTGCAGAGGTCTTCCCCTGTGTTTCTGCAACCTGTGGTCAATTTTACCATCCTCGTTGTGTTGCACAACTAATTTATCAAGACAATGGAGTTTCTGCTGAAGAACTGGTGAAAAATATCTCTCTGGGGGAGTCTTTTACGTGTCCAATTCATAAGTGCTGTGTTTGTAAGCAAGGAGAGAATAAGAAGGATCCCGAGTTGCGGTTTGCTGTGTGCAGGCGTTGTCCTAAATCCTATCACAGGAAATGCTTGCCCAGTGAGATTCGTTTTGAGAAAACAGAGGAAGATGATGAGGAAGAGATAGAGGATGAAGAAGAAATAGAAACAAGAGCTTGGGAAGGTCTATTACCTAATCGCATACTAATATATTGCACAGAACACGAAATAGATGAAGAAATTGGAACTCCAATAAGGGACCATGTTAAATTTCCCGATGTTGACGGAAAGAAGACTAGTATTGTGAAAAGGATGACTGCTTTTGAAGAGAAAAAGAGAAGACTGACATCAGAGTCCCTTGTGGTCAGAGATAATTCTCTGTCGAAGAAAAGAAAGCTTTCCTCAGAGGGATTTTATAGTGGAAGAACTGCACCTCCAATATCTAAGCAGAAATTGAATTCGGGTGAAAAAGTTGGCAGTAGCAGAAATACTGAAAAAGTACCTTCTCGGTTAGACGTATCGAGAAAGGTGATGGTTAAGAAGACATTGAAAAAGGAAGTTCAGACTAGCGTGGAAAAGAAGACCTCGTTGGGTAATAGACTGTTTACCTACATAAAGGAATCTGGGTCAGTAAAGTTTGGGAAGCAGGATACACCTGATGATGCTGAACTAAATTCTGAAACGAGTGCAAAAATTGATCCTACATCAAGAACATTGAGTGCGGCAGCTTCCTTAGATCCTGCTAGAGAAAGGAGATTACATGCTTTAATGAAAGATGCTGCTTCGTCAATAAGATTGGAAGATGTTATAGAAAAGCACAAAGTTCCATCCACTCATGCATCCTCAAATAAATATGCTGTAGAAAGGAACATAACACGAGGAAAGGTGGAAGGCTCAGTTGAGGCTGTTCGAACCGCATTAAAGAAACTACAGGGAGGCTGCAGTACTGAAGATGCAGCGGCTGTCTGTGCTCCCGAGATTCTTTCCCAGATTTATAAGTGGAAGAGCAAGCTTAGAGTATATCTTGCACCTTTCTTGCATGGAAAGCGGTACACGTCCTTCGGTCGCCATTTCACAAAGGTGGAAAAACTCGAACAGATTGCTGATAGGCTCCATCCGTACGTACAGAATGGAGACACGATAGTGGACTTCTGTTGTGGCTCTAATGATTTCAGCATCGTAATGAAGAAAAAGCTTGAGGAGACAGGGAAGAAATGCTATTTTAGAAATTATGACATCATTCAACCAAAGAATGATTTCTGTTTTGAGAAGAGAGATTGGATGACTGTTCAAAAAGGTGAATTACCGGATAGTAGTAAATTGATCATGGGGTTGAACCCTCCTTTTGGAGTTAAAGCATCTTTGGCAAACCAGTTTATTGATAAGGCTCTGCAGTTCCAGCCGAAGCTCCTTATTCTTATTGTTCCACCAGAAACAAAAGGGTTACAATGTTATGATCTGATATGGAAGGATGAACGGTCTCTGTCGGGAAAGTCCTTTTATCTTCCTGGATCTGTTGATGAGAATGACAAGCAAATGGATCAGTGGAATGTGACGACACCTCCACTCTATCTCTGGAGTCGTCCTGATTGGTCTGAGAGACACAGAGCTATTGCTCAAAAGGATAGTCACGGTTCCATGTCACAAGGGTCAGTGGAAGATGGTCATTCAGTTGAGAATAAGGATGAAAATGTTGAAAATCTCATGTTGAAAAATGCTTATCTGACACCGACCGGTAATTCCAGAGATTTTGTTGGTGGGGCAGTTGCAGGCGAGGGACATGAAGATAGATCCTCCAAGGGCAATGATGACAGAGGTTACCATGCGAGTTCTGGTCACCAGAAGAACCAGGTTAGTAAGGTTTCTGAGAGGCCGCGGTGTGGTGGCAGAAAAAAGAATGGGACTGGAGTGGTTGAGTTGTCGCCAGACAACAAATGTGATGGTGGCAATGTTTCTAGTGAGATTCAGATGGAAAGGTCTCCATCTACTGAGCGACAGCGGAAAGCAAATCAGCAGTCTGAGCCCAGCGATTCTGGTTCAAGTATGCATTTCAGAACTGCTTGTGGGAGGACCACAGCCAGCATCCCTGATGACACTGGCAGCAATGTCACAAGCAGTGCTGACCCTTATTCAAGTGTGACTCATAGATGGTCCAGTGGTGCCAGTCCTAGCTCTATATATGGGGATACAAAATTGGAGGAACCTGTTTTGGGTTACATGAGAGAGACAGATAACTTTGGTTATAGACCGTATTCCATGGGGATGGAAGACCCATATAGGAGAGACTCTGATATACGCGCACAAGTTCGCCATTACGGGCAGCAAGATCCTGATATGTTCAGAAGTAATTATCTAGCAGGTCAGGATTTGTTACGTGGTCGGGTTGGATCATTTTCGTCCACGTATGGCCATGCTCATATAGGTTCAGCAGCTGACTCATCTTACAGAATGAACATGTCAGCCATGCAGCGATATGAACCTCGGCTGGATGAATTGAATCACACAAGAGCGAGTGCCTTAGGAACCCAACCGGCGTTTGGGTATGATCCACGCACATACAGTAGCAACATGTATGATCCCAGGGCACCCCAACCTGGTGACCAAGCGGGCCCCTATGGTTTTGCTCCCGGTTCTCACCAAAGCTTCTCAAACCGGAATTCAGCAGGTTGGCTGAACGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006325 GO:0006355 GO:0006950 GO:0006952 GO:0006996 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0009605 GO:0009607 GO:0009620 GO:0009791 GO:0009889 GO:0009893 GO:0009909 GO:0009911 GO:0009987 GO:0010228 GO:0010468 GO:0010556 GO:0010604 GO:0010638 GO:0016043 GO:0019219 GO:0019222 GO:0022414 GO:0023052 GO:0030154 GO:0031056 GO:0031058 GO:0031060 GO:0031062 GO:0031323 GO:0031325 GO:0031326 GO:0031399 GO:0031401 GO:0031440 GO:0031935 GO:0031937 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033043 GO:0033044 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0043954 GO:0044030 GO:0044424 GO:0044464 GO:0048366 GO:0048367 GO:0048468 GO:0048518 GO:0048522 GO:0048580 GO:0048582 GO:0048608 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0048869 GO:0050684 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051052 GO:0051094 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051276 GO:0051570 GO:0051574 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060968 GO:0061085 GO:0061087 GO:0061458 GO:0065007 GO:0070827 GO:0070828 GO:0070829 GO:0071840 GO:0080090 GO:0090436 GO:0097159 GO:0098542 GO:0099402 GO:0140110 GO:1900109 GO:1900111 GO:1900363 GO:1901363 GO:1902275 GO:1903311 GO:1903506 GO:1905269 GO:2000024 GO:2000026 GO:2000112 GO:2000241 GO:2000243 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNMT1-RFD

Protein Analysis

1245

Amino Acids

139.73

Weight (kDa)

5.92

Isoelectric Point (pI)

46.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNMT1-RFD PF12047 13 - 111 7.1e-07 Cytosine specific DNA methyltransferase replication foci domain
PHD_NSD PF22908 283 - 351 1e-19 Histone-lysine N-methyltransferase NSD-like, PHD zinc finger
Mtase_EDM2 PF26055 726 - 892 4.2e-88 EDM2 methyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000343)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48090 AT5G48090 AT5G48090 AT5G55390 AT5G55390 AT5G55390
fragaria_vesca FvH4_3g23690 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160
malus_domestica MD03G1191000.v1.1 MD11G1207800.v1.1 MD11G1208000.v1.1
prunus_persica Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227600_v2.0.a1 Prupe.4G227700_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1
pyrus_communis pycom01g03400 pycom03g14420 pycom11g18100 pycom11g18140 pycom11g18150 pycom11g18160
rosa_chinensis RchiOBHm_Chr2g0145981 RchiOBHm_Chr4g0405241 RchiOBHm_Chr4g0405271 RchiOBHm_Chr5g0016201 RchiOBHm_Chr5g0041831 RchiOBHm_Chr5g0041871 RchiOBHm_Chr5g0041881 RchiOBHm_Chr5g0059241
rosa_laevigata RLG00000002117 RLG00000008861 RLG00000008862 RLG00000011000 RLG00000033216 RLG00000034097 RLG00000034101
rosa_multiflora Rmu_sc0000161.1_g000001 Rmu_sc0000861.1_g000040 Rmu_sc0007522.1_g000003 Rmu_sc0009443.1_g000015 Rmu_sc0022129.1_g000001 Rmu_sc0042315.1_g000001
rosa_roxburghii Rroxscaffold_1G00038660 Rroxscaffold_1G00038690 Rroxscaffold_5G00349560 Rroxscaffold_5G00349610 Rroxscaffold_6G00388370 Rroxscaffold_7G00188740
rosa_rugosa Rorug03G0199700 Rorug04G0055400 Rorug04G0055500 Rorug04G0055900 Rorug04G0056000 Rorug04G0056100 Rorug04G0056100 Rorug04G0056200 Rorug05G0195600 Rorug05G0195700 Rorug05G0195800 Rorug05G0195900 Rorug05G0196800 Rorug05G0385000
rosa_samantha Rh4AG129900 Rh4AG130100 Rh4BG124200 Rh4BG124400 Rh4BG124500 Rh4CG137200 Rh4CG137300 Rh4DG123500 Rh4DG123700 Rh5AG280800 Rh5AG281400 Rh5BG286700 Rh5BG287000 Rh5BG397800 Rh5CG318100 Rh5CG318600 Rh5CG421600 Rh5DG132300 Rh5DG132400 Rh5DG295400 Rh5DG295700 Rh7BG475300
rosa_wichuraiana Rw0G001330 Rw0G005730 Rw4G008670 Rw4G010510 Rw4G010540 Rw5G026450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 512, 2052
AasI GACNNNNNNGTC 1 cut(s) 1408
Acc36I ACCTGC 2 cut(s) 3404, 3710
AccB1I GGYRCC 2 cut(s) 3215, 3640
AccII CGCG 2 cut(s) 2932, 3354
AcoI YGGCCR 1 cut(s) 3463
AcsI RAATTY 4 cut(s) 1325, 1513, 1729, 3712
AcuI CTGAAG 2 cut(s) 1020, 2025
AcyI GRCGYC 1 cut(s) 368
AfaI GTAC 5 cut(s) 1557, 2002, 2108, 2177, 2181
AfiI CCNNNNNNNGG 9 cut(s) 546, 748, 1101, 1140, 2402, 2936, 3266, 3593, 3650
AflIII ACRYGT 4 cut(s) 1040, 2109, 3510, 3624
AgeI ACCGGT 1 cut(s) 2789
AhlI ACTAGT 1 cut(s) 1352
AjiI CACGTC 1 cut(s) 2112
AjnI CCWGG 4 cut(s) 2563, 2904, 3635, 3649
AjuI GAANNNNNNNTTGG 2 cut(s) 1628, 1660
AleI CACNNNNGTG 2 cut(s) 2135, 2198
AloI GAACNNNNNNTCC 2 cut(s) 1474, 1506
Alw21I GWGCWC 1 cut(s) 2029
Alw26I GTCTC 7 cut(s) 2184, 2252, 2541, 2650, 3039, 3275, 3333
AlwNI CAGNNNCTG 2 cut(s) 2018, 3488
Ama87I CYCGRG 5 cut(s) 179, 236, 795, 1094, 2030
AoxI GGCC 4 cut(s) 425, 2927, 3463, 3664
ApaI GGGCCC 1 cut(s) 3668
ApoI RAATTY 4 cut(s) 1325, 1513, 1729, 3712
ArsI GACNNNNNNTTYG 4 cut(s) 2668, 2700, 3056, 3088
AsiGI ACCGGT 1 cut(s) 2789
Asp700I GAANNNNTTC 2 cut(s) 1453, 2312
AspLEI GCGC 1 cut(s) 3356
AspS9I GGNCC 6 cut(s) 735, 1315, 3126, 3207, 3664, 3665
AsuC2I CCSGG 1 cut(s) 3684
AsuHPI GGTGA 9 cut(s) 131, 458, 1021, 1532, 1597, 2366, 2885, 3665, 3683
AsuII TTCGAA 3 cut(s) 47, 224, 1966
AvaI CYCGRG 5 cut(s) 179, 236, 795, 1094, 2030
AvaII GGWCC 4 cut(s) 735, 1315, 3126, 3207
AxyI CCTNAGG 2 cut(s) 731, 3574
BaeGI GKGCMC 2 cut(s) 3643, 3668
BalI TGGCCA 1 cut(s) 3465
BamHI GGATCC 1 cut(s) 1090
BanI GGYRCC 2 cut(s) 3215, 3640
BanII GRGCYC 4 cut(s) 180, 460, 3081, 3668
BarI GAAGNNNNNNTAC 4 cut(s) 1230, 1262, 2099, 2131
BauI CACGAG 1 cut(s) 1932
BbsI GAAGAC 6 cut(s) 904, 1355, 1402, 1604, 1643, 3330
Bbv12I GWGCWC 1 cut(s) 2029
BbvCI CCTCAGC 2 cut(s) 24, 363
BceAI ACGGC 1 cut(s) 775
BcgI CGANNNNNNTGC 2 cut(s) 2138, 2172
BciT130I CCWGG 4 cut(s) 2565, 2906, 3637, 3651
BciVI GTATCC 2 cut(s) 1699, 3235
BclI TGATCA 2 cut(s) 448, 2379
BcnI CCSGG 1 cut(s) 3684
BcoDI GTCTC 7 cut(s) 2184, 2252, 2541, 2650, 3039, 3275, 3333
BcuI ACTAGT 1 cut(s) 1352
BfaI CTAG 6 cut(s) 1353, 1623, 1800, 3012, 3225, 3410
BfmI CTRYAG 5 cut(s) 576, 1914, 1985, 1996, 2447
BfuAI ACCTGC 2 cut(s) 3404, 3710
BfuI GTATCC 2 cut(s) 1699, 3235
BmcAI AGTACT 1 cut(s) 2002
Bme1390I CCNGG 5 cut(s) 2565, 2906, 3637, 3651, 3684
Bme18I GGWCC 4 cut(s) 735, 1315, 3126, 3207
BmeT110I CYCGRG 5 cut(s) 179, 236, 795, 1094, 2030
BmgBI CACGTC 1 cut(s) 2112
BmgT120I GGNCC 6 cut(s) 735, 1315, 3126, 3207, 3664, 3665
BmrFI CCNGG 5 cut(s) 2565, 2906, 3637, 3651, 3684
BmrI ACTGGG 1 cut(s) 1149
BmuI ACTGGG 1 cut(s) 1149
BpiI GAAGAC 6 cut(s) 904, 1355, 1402, 1604, 1643, 3330
BplI GAGNNNNNCTC 2 cut(s) 68, 100
BpmI CTGGAG 3 cut(s) 263, 2653, 2982
Bpu10I CCTNAGC 2 cut(s) 24, 363
Bpu14I TTCGAA 3 cut(s) 47, 224, 1966
BpuEI CTTGAG 1 cut(s) 2273
BpuMI CCSGG 1 cut(s) 3684
BsaAI YACGTR 4 cut(s) 1041, 2179, 3431, 3459
BsaHI GRCGYC 1 cut(s) 368
BsaI GGTCTC 2 cut(s) 2541, 3039
BsaWI WCCGGW 3 cut(s) 2362, 2789, 3708
BsaXI ACNNNNNCTCC 6 cut(s) 103, 133, 1474, 1504, 2602, 2632
Bsc4I CCNNNNNNNGG 9 cut(s) 546, 748, 1101, 1140, 2402, 2936, 3266, 3593, 3650
Bse118I RCCGGY 2 cut(s) 2789, 3586
Bse1I ACTGG 7 cut(s) 1011, 1155, 2428, 2965, 3157, 3210, 3219
Bse21I CCTNAGG 2 cut(s) 731, 3574
Bse3DI GCAATG 6 cut(s) 36, 357, 819, 2866, 3010, 3166
BseBI CCWGG 4 cut(s) 2565, 2906, 3637, 3651
BseLI CCNNNNNNNGG 9 cut(s) 546, 748, 1101, 1140, 2402, 2936, 3266, 3593, 3650
BseMI GCAATG 6 cut(s) 36, 357, 819, 2866, 3010, 3166
BseMII CTCAG 9 cut(s) 15, 354, 900, 1473, 1965, 2643, 2913, 3036, 3066
BseNI ACTGG 7 cut(s) 1011, 1155, 2428, 2965, 3157, 3210, 3219
BseRI GAGGAG 4 cut(s) 304, 307, 874, 2270
BseSI GKGCMC 2 cut(s) 3643, 3668
BseYI CCCAGC 1 cut(s) 3080
BsgI GTGCAG 4 cut(s) 353, 924, 1135, 1470
Bsh1236I CGCG 2 cut(s) 2932, 3354
Bsh1285I CGRYCG 2 cut(s) 2122, 2790
BshFI GGCC 4 cut(s) 427, 2929, 3465, 3666
BshNI GGYRCC 2 cut(s) 3215, 3640
BshTI ACCGGT 1 cut(s) 2789
BsiEI CGRYCG 2 cut(s) 2122, 2790
BsiHKAI GWGCWC 1 cut(s) 2029
BsiHKCI CYCGRG 5 cut(s) 179, 236, 795, 1094, 2030
BsiSI CCGG 5 cut(s) 2363, 2790, 3587, 3684, 3709
BsiWI CGTACG 1 cut(s) 2175
BslFI GGGAC 4 cut(s) 1328, 1396, 2847, 2971
BslI CCNNNNNNNGG 9 cut(s) 546, 748, 1101, 1140, 2402, 2936, 3266, 3593, 3650
BsmAI GTCTC 7 cut(s) 2184, 2252, 2541, 2650, 3039, 3275, 3333
BsmFI GGGAC 4 cut(s) 1328, 1396, 2847, 2971
BsmI GAATGC 1 cut(s) 364
BsnI GGCC 4 cut(s) 427, 2929, 3465, 3666
Bso31I GGTCTC 2 cut(s) 2541, 3039
BsoBI CYCGRG 5 cut(s) 179, 236, 795, 1094, 2030
Bsp119I TTCGAA 3 cut(s) 47, 224, 1966
Bsp120I GGGCCC 1 cut(s) 3664
Bsp1286I GDGCHC 6 cut(s) 180, 460, 2029, 3081, 3643, 3668
Bsp19I CCATGG 1 cut(s) 3312
BspANI GGCC 4 cut(s) 427, 2929, 3465, 3666
BspCNI CTCAG 9 cut(s) 16, 355, 899, 1472, 1964, 2644, 2914, 3037, 3067
BspFNI CGCG 2 cut(s) 2932, 3354
BspMAI CTGCAG 2 cut(s) 2000, 2451
BspMI ACCTGC 2 cut(s) 3404, 3710
BspQI GCTCTTC 3 cut(s) 69, 525, 2054
BspT104I TTCGAA 3 cut(s) 47, 224, 1966
BspT107I GGYRCC 2 cut(s) 3215, 3640
BspTNI GGTCTC 2 cut(s) 2541, 3039
BsrDI GCAATG 6 cut(s) 36, 357, 819, 2866, 3010, 3166
BsrFI RCCGGY 2 cut(s) 2789, 3586
BsrI ACTGG 7 cut(s) 1011, 1155, 2428, 2965, 3157, 3210, 3219
BssAI RCCGGY 2 cut(s) 2789, 3586
BssNI GRCGYC 1 cut(s) 368
BssSI CACGAG 1 cut(s) 1932
BssT1I CCWWGG 4 cut(s) 255, 492, 2853, 3312
Bst2BI CACGAG 1 cut(s) 1932
Bst2UI CCWGG 4 cut(s) 2565, 2906, 3637, 3651
Bst6I CTCTTC 6 cut(s) 69, 525, 1191, 1377, 2054, 2321
BstACI GRCGYC 1 cut(s) 368
BstBAI YACGTR 4 cut(s) 1041, 2179, 3431, 3459
BstBI TTCGAA 3 cut(s) 47, 224, 1966
BstC8I GCNNGC 7 cut(s) 704, 1118, 1151, 2066, 2827, 3136, 3664
BstDSI CCRYGG 3 cut(s) 747, 2930, 3312
BstEII GGTNACC 3 cut(s) 2873, 2891, 3653
BstENI CCTNNNNNAGG 1 cut(s) 1138
BstFNI CGCG 2 cut(s) 2932, 3354
BstHHI GCGC 1 cut(s) 3356
BstMAI GTCTC 7 cut(s) 2184, 2252, 2541, 2650, 3039, 3275, 3333
BstMCI CGRYCG 2 cut(s) 2122, 2790
BstMWI GCNNNNNNNGC 7 cut(s) 283, 433, 2024, 2101, 2420, 2455, 3076
BstNI CCWGG 4 cut(s) 2565, 2906, 3637, 3651
BstNSI RCATGY 4 cut(s) 415, 1820, 3514, 3628
BstPI GGTNACC 3 cut(s) 2873, 2891, 3653
BstSCI CCNGG 5 cut(s) 2563, 2904, 3635, 3649, 3682
BstSFI CTRYAG 5 cut(s) 576, 1914, 1985, 1996, 2447
BstSLI GKGCMC 2 cut(s) 3643, 3668
BstSNI TACGTA 1 cut(s) 2179
BstUI CGCG 2 cut(s) 2932, 3354
BstV2I GAAGAC 6 cut(s) 904, 1355, 1402, 1604, 1643, 3330
BstX2I RGATCY 5 cut(s) 1090, 1792, 2567, 2846, 3382
BstXI CCANNNNNNTGG 2 cut(s) 108, 3319
BstYI RGATCY 5 cut(s) 1090, 1792, 2567, 2846, 3382
Bsu36I CCTNAGG 2 cut(s) 731, 3574
BsuI GTATCC 2 cut(s) 1699, 3235
BsuRI GGCC 4 cut(s) 427, 2929, 3465, 3666
BtgI CCRYGG 3 cut(s) 747, 2930, 3312
BtrI CACGTC 1 cut(s) 2112
BtsI GCAGTG 5 cut(s) 150, 169, 252, 341, 3178
BveI ACCTGC 2 cut(s) 3404, 3710
Cac8I GCNNGC 7 cut(s) 704, 1118, 1151, 2066, 2827, 3136, 3664
CaiI CAGNNNCTG 2 cut(s) 2018, 3488
CfoI GCGC 1 cut(s) 3356
Cfr10I RCCGGY 2 cut(s) 2789, 3586
Cfr13I GGNCC 6 cut(s) 735, 1315, 3126, 3207, 3664, 3665
Cfr42I CCGCGG 1 cut(s) 2933
CseI GACGC 1 cut(s) 357
CsiI ACCWGGT 2 cut(s) 2904, 3649
Csp6I GTAC 5 cut(s) 1556, 2001, 2107, 2176, 2180
CspAI ACCGGT 1 cut(s) 2789
CviQI GTAC 5 cut(s) 1556, 2001, 2107, 2176, 2180
DrdI GACNNNNNNGTC 1 cut(s) 1408
DseDI GACNNNNNNGTC 1 cut(s) 1408
EaeI YGGCCR 1 cut(s) 3463
Eam1104I CTCTTC 6 cut(s) 69, 525, 1191, 1377, 2054, 2321
EarI CTCTTC 6 cut(s) 69, 525, 1191, 1377, 2054, 2321
Eco105I TACGTA 1 cut(s) 2179
Eco130I CCWWGG 4 cut(s) 255, 492, 2853, 3312
Eco147I AGGCCT 1 cut(s) 427
Eco24I GRGCYC 4 cut(s) 180, 460, 3081, 3668
Eco31I GGTCTC 2 cut(s) 2541, 3039
Eco47I GGWCC 4 cut(s) 735, 1315, 3126, 3207
Eco57I CTGAAG 2 cut(s) 1020, 2025
Eco81I CCTNAGG 2 cut(s) 731, 3574
Eco88I CYCGRG 5 cut(s) 179, 236, 795, 1094, 2030
Eco91I GGTNACC 3 cut(s) 2873, 2891, 3653
EcoNI CCTNNNNNAGG 1 cut(s) 1138
EcoO109I RGGNCCY 2 cut(s) 735, 3665
EcoO65I GGTNACC 3 cut(s) 2873, 2891, 3653
EcoRI GAATTC 2 cut(s) 1513, 3712
EcoRII CCWGG 4 cut(s) 2563, 2904, 3635, 3649
EcoT14I CCWWGG 4 cut(s) 255, 492, 2853, 3312
EcoT22I ATGCAT 2 cut(s) 1897, 3105
EcoT38I GRGCYC 4 cut(s) 180, 460, 3081, 3668
ErhI CCWWGG 4 cut(s) 255, 492, 2853, 3312
FaqI GGGAC 4 cut(s) 1328, 1396, 2847, 2971
FauI CCCGC 1 cut(s) 3655
FbaI TGATCA 2 cut(s) 448, 2379
FriOI GRGCYC 4 cut(s) 180, 460, 3081, 3668
FspBI CTAG 6 cut(s) 1353, 1623, 1800, 3012, 3225, 3410
GlaI GCGC 1 cut(s) 3355
GsaI CCCAGC 1 cut(s) 3084
GsuI CTGGAG 3 cut(s) 263, 2653, 2982
HaeIII GGCC 4 cut(s) 427, 2929, 3465, 3666
HapII CCGG 5 cut(s) 2363, 2790, 3587, 3684, 3709
HgaI GACGC 1 cut(s) 357
HhaI GCGC 1 cut(s) 3356
Hin1I GRCGYC 1 cut(s) 368
Hin6I GCGC 1 cut(s) 3354
HinP1I GCGC 1 cut(s) 3354
HincII GTYRAC 1 cut(s) 1339
HindII GTYRAC 1 cut(s) 1339
HindIII AAGCTT 5 cut(s) 584, 1451, 2066, 2249, 3697
HpaII CCGG 5 cut(s) 2363, 2790, 3587, 3684, 3709
HphI GGTGA 9 cut(s) 131, 458, 1021, 1532, 1597, 2366, 2885, 3665, 3683
Hpy166II GTNNAC 5 cut(s) 1339, 1662, 2109, 2203, 3456
Hpy8I GTNNAC 5 cut(s) 1339, 1662, 2109, 2203, 3456
HpyCH4IV ACGT 6 cut(s) 1040, 1572, 2111, 2178, 3430, 3458
HpyF10VI GCNNNNNNNGC 7 cut(s) 283, 433, 2024, 2101, 2420, 2455, 3076
HpySE526I ACGT 6 cut(s) 1040, 1572, 2111, 2178, 3430, 3458
Hsp92I GRCGYC 1 cut(s) 368
HspAI GCGC 1 cut(s) 3354
Ksp22I TGATCA 2 cut(s) 448, 2379
KspI CCGCGG 1 cut(s) 2933
LguI GCTCTTC 3 cut(s) 69, 525, 2054
LmnI GCTCC 7 cut(s) 282, 294, 887, 2032, 2172, 2469, 3685
MabI ACCWGGT 2 cut(s) 2904, 3649
MaeI CTAG 6 cut(s) 1353, 1623, 1800, 3012, 3225, 3410
MaeII ACGT 6 cut(s) 1040, 1572, 2111, 2178, 3430, 3458
MflI RGATCY 5 cut(s) 1090, 1792, 2567, 2846, 3382
MhlI GDGCHC 6 cut(s) 180, 460, 2029, 3081, 3643, 3668
MlsI TGGCCA 1 cut(s) 3465
MluNI TGGCCA 1 cut(s) 3465
MlyI GAGTC 8 cut(s) 647, 761, 1040, 1418, 2644, 3190, 3335, 3485
MmeI TCCRAC 1 cut(s) 3421
Mox20I TGGCCA 1 cut(s) 3465
Mph1103I ATGCAT 2 cut(s) 1897, 3105
MroXI GAANNNNTTC 2 cut(s) 1453, 2312
MscI TGGCCA 1 cut(s) 3465
MseI TTAA 6 cut(s) 525, 1323, 1593, 1823, 1976, 2409
MslI CAYNNNNRTG 5 cut(s) 162, 2135, 2198, 2237, 3317
Msp20I TGGCCA 1 cut(s) 3465
MspA1I CMGCKG 4 cut(s) 2015, 2932, 3055, 3488
MspI CCGG 5 cut(s) 2363, 2790, 3587, 3684, 3709
MspR9I CCNGG 5 cut(s) 2565, 2906, 3637, 3651, 3684
Mva1269I GAATGC 1 cut(s) 364
MvaI CCWGG 4 cut(s) 2565, 2906, 3637, 3651
MvnI CGCG 2 cut(s) 2932, 3354
MwoI GCNNNNNNNGC 7 cut(s) 283, 433, 2024, 2101, 2420, 2455, 3076
NciI CCSGG 1 cut(s) 3684
NcoI CCATGG 1 cut(s) 3312
NmeAIII GCCGAG 2 cut(s) 312, 3517
NmuCI GTSAC 7 cut(s) 2608, 2684, 2697, 2891, 3163, 3193, 3653
NsiI ATGCAT 2 cut(s) 1897, 3105
NspI RCATGY 4 cut(s) 415, 1820, 3514, 3628
NspV TTCGAA 3 cut(s) 47, 224, 1966
OliI CACNNNNGTG 2 cut(s) 2135, 2198
PaeR7I CTCGAG 1 cut(s) 179
PasI CCCWGGG 1 cut(s) 3636
PceI AGGCCT 1 cut(s) 427
PciI ACATGT 2 cut(s) 3510, 3624
PciSI GCTCTTC 3 cut(s) 69, 525, 2054
PctI GAATGC 1 cut(s) 364
PdmI GAANNNNTTC 2 cut(s) 1453, 2312
Pfl23II CGTACG 1 cut(s) 2175
PfoI TCCNGGA 1 cut(s) 2563
PinAI ACCGGT 1 cut(s) 2789
PleI GAGTC 8 cut(s) 646, 761, 1039, 1417, 2643, 3190, 3335, 3485
PpsI GAGTC 8 cut(s) 646, 761, 1039, 1417, 2643, 3190, 3335, 3485
Ppu21I YACGTR 4 cut(s) 1041, 2179, 3431, 3459
PpuMI RGGWCCY 1 cut(s) 735
PscI ACATGT 2 cut(s) 3510, 3624
PsiI TTATAA 2 cut(s) 512, 2052
Psp5II RGGWCCY 1 cut(s) 735
Psp6I CCWGG 4 cut(s) 2563, 2904, 3635, 3649
PspEI GGTNACC 3 cut(s) 2873, 2891, 3653
PspFI CCCAGC 1 cut(s) 3080
PspGI CCWGG 4 cut(s) 2563, 2904, 3635, 3649
PspLI CGTACG 1 cut(s) 2175
PspOMI GGGCCC 1 cut(s) 3664
PspPI GGNCC 6 cut(s) 735, 1315, 3126, 3207, 3664, 3665
PspPPI RGGWCCY 1 cut(s) 735
PstI CTGCAG 2 cut(s) 2000, 2451
PstNI CAGNNNCTG 2 cut(s) 2018, 3488
PsuI RGATCY 5 cut(s) 1090, 1792, 2567, 2846, 3382
PvuII CAGCTG 1 cut(s) 3488
RsaI GTAC 5 cut(s) 1557, 2002, 2108, 2177, 2181
RsaNI GTAC 5 cut(s) 1556, 2001, 2107, 2176, 2180
RseI CAYNNNNRTG 5 cut(s) 162, 2135, 2198, 2237, 3317
SacII CCGCGG 1 cut(s) 2933
SapI GCTCTTC 3 cut(s) 69, 525, 2054
SaqAI TTAA 6 cut(s) 525, 1323, 1593, 1823, 1976, 2409
Sau96I GGNCC 6 cut(s) 735, 1315, 3126, 3207, 3664, 3665
ScaI AGTACT 1 cut(s) 2002
SchI GAGTC 8 cut(s) 647, 761, 1040, 1418, 2644, 3190, 3335, 3485
ScrFI CCNGG 5 cut(s) 2565, 2906, 3637, 3651, 3684
SduI GDGCHC 6 cut(s) 180, 460, 2029, 3081, 3643, 3668
SexAI ACCWGGT 2 cut(s) 2904, 3649
SfcI CTRYAG 5 cut(s) 576, 1914, 1985, 1996, 2447
Sfr274I CTCGAG 1 cut(s) 179
Sfr303I CCGCGG 1 cut(s) 2933
SfuI TTCGAA 3 cut(s) 47, 224, 1966
SgrBI CCGCGG 1 cut(s) 2933
SinI GGWCC 4 cut(s) 735, 1315, 3126, 3207
SlaI CTCGAG 1 cut(s) 179
SmiMI CAYNNNNRTG 5 cut(s) 162, 2135, 2198, 2237, 3317
SmlI CTYRAG 2 cut(s) 179, 2252
SmoI CTYRAG 2 cut(s) 179, 2252
SnaBI TACGTA 1 cut(s) 2179
SpeI ACTAGT 1 cut(s) 1352
SseBI AGGCCT 1 cut(s) 427
SspMI CTAG 6 cut(s) 1353, 1623, 1800, 3012, 3225, 3410
StuI AGGCCT 1 cut(s) 427
StyD4I CCNGG 5 cut(s) 2563, 2904, 3635, 3649, 3682
StyI CCWWGG 4 cut(s) 255, 492, 2853, 3312
TaiI ACGT 6 cut(s) 1043, 1575, 2114, 2181, 3433, 3461
TaqI TCGA 8 cut(s) 47, 180, 224, 503, 1439, 1577, 1966, 2148
TaqII GACCGA 1 cut(s) 2108
TatI WGTACW 1 cut(s) 2000
TauI GCSGC 3 cut(s) 1781, 2018, 2932
Tru1I TTAA 6 cut(s) 525, 1323, 1593, 1823, 1976, 2409
Tru9I TTAA 6 cut(s) 525, 1323, 1593, 1823, 1976, 2409
TseFI GTSAC 7 cut(s) 2608, 2684, 2697, 2891, 3163, 3193, 3653
Tsp45I GTSAC 7 cut(s) 2608, 2684, 2697, 2891, 3163, 3193, 3653
TspGWI ACGGA 2 cut(s) 1356, 2163
VpaK11BI GGWCC 4 cut(s) 735, 1315, 3126, 3207
XagI CCTNNNNNAGG 1 cut(s) 1138
XapI RAATTY 4 cut(s) 1325, 1513, 1729, 3712
XceI RCATGY 4 cut(s) 415, 1820, 3514, 3628
XcmI CCANNNNNNNNNTGG 3 cut(s) 2628, 2885, 3590
XhoI CTCGAG 1 cut(s) 179
XmnI GAANNNNTTC 2 cut(s) 1453, 2312
XspI CTAG 6 cut(s) 1353, 1623, 1800, 3012, 3225, 3410
ZrmI AGTACT 1 cut(s) 2002
Zsp2I ATGCAT 2 cut(s) 1897, 3105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.