Rh4BG124500

metal ion binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0000343
Physical Location & Seq
Forward (+)
20812775 .. 20818087
5313 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG124500.1

Sequence Viewer

Length: 2877 bp
ATGGCATCGGCAGAGTGTCAACCATATTCTGTTTCGGAGTACCATTTCGTAGATGAAAAGGATGTATGTGTCTCATTTCATGTATTGCCTGTTCAATGGAGTGATGGTGAGAGACTGGATGGCGAGAATGTAAAGATTTTTCTGCATGGAACCGCTGATAATGGGGGGGATTTATGTAAGCATGTTATTGCATGGAAGTTTGACATTTCGAGTGAAGAGCCTGAGATATTGGTTCTCTCAGAGGAAAATAGTTGGATCAAGCTTGGGAAGCCGAGGAAGAGCTTTGAGGACACTGTTAGGTCGATCTTGATAACGGTGCACTGCCTTCACTATGTGAAGAGAAATCCGGAAAAATCCAGAGAATGTGTGTGGAATCACCTATCTGAAGTTTTCAGGTTGTATGTGGTCAGGCCTTCTCAAAGTGATTTGGTGGATCACAGGCCTCTAATCGGTGATTGTGTTAACAGATATCGTTCCCTTGCGGATTCCAAGTTTTTGGCCAAACTTTTTGAAGAGAAGACAATGAAGAAGAAACTCTCTGATGAGGATACTGAAGCTAGAAAGAAGCTCAGAATTATGATCGATGATGTAAAAGATGATATGATTCATGCAGAAGATGAGGATATCGATAACCTGTTTGGTGAGGTCTGTGCTTTATGTGACGATGGTGGCAAGATCATTTGTTGTGAAGGGGGGTGCCTAAGGTCTTTTCATGCAACTGAGGAGGCGGGTGCCGGATCTTATTGCAAATCTCTTGGCTTTATGCAGGATGATGTTGTTGGGATGCAAAATTTCTACTGCAAGAACTGTGAACATAAACAACACCAGTGCTTTGTTTGTGGGAAGTTAGGTTCCTCTGATAAGTCCTCAGGTGCTGCTGAGGTGGTTTCCTGTGTATCCCCAAACTGTGGTCGATTTTACCATCCACATTGTGTTGCAATATTACTTTACCAAGATAATGGAGTTCCTGCTGAAGAACTTGAGAGAAAGATTGCTGGTGGGGAATCTTTTACTTGTCCAATTCATAAGTGCTGCATTTGTAAACAAGGAGAAAATAAGAAGGATAGTGAGTTACAATTTGCTGTGTGCATGCGTTGTCCAAAATCGTACCACAGGAAATGCTTGCCAAGGGAGATTGCTTTTGAAAATCAAGGAGAAATGCTAGAAGGGAGATCAATAATAAGAGCTTGGGATGGTCTATTACCTAACCGTGTACTCATATATTGCACAAAACATGATATAGATATTGAATTTGGAACTGTAAGAAGGGACCACATAAAATTCCCTGATGTCAAACAGAATATGAGCACTCTCAAAAAGAAGAAGAAGACTATTTTAGAAGAGAAGTGGAAGCCAATGTCAGAATTCCTTGCAGACGGAGACAAAGTTGTGACTAAGAGAAATATTTCCTTGGATAAAAATGCTCCTACAGCACTTAAGACACAAAAGCCATATGTTCAAGAGAAGAAGACTAGGAAACTTATGTCAGAGCAGAAACCTGTGTCTAAGAAGAGCGATTCTTTTTTGGAAGAATCAGTTGGGGAAGGAAGTGATCCTGTACTATCCACACTAGTCAAGCAGAAGCTTTATTCAGTTAAAGAGAAGAAGGCTACTTTTGTAAAAGAGAAGACTTTTGTTGAAGAGAAAGAAAGCCAACTGACATCAACGGTCTCTGTAGACAGAGAAAAAGCTGTGTCGAAGAGGAGAACTCCTTCCTCTGAAGGACTATATAGTGAGAAAAGTGCTCCTACAATATCCAAGAAAAAACAGAAGCCATCTTTTGTTGAAGAAAAGAACAATAACCTTGTAAAAAAGAAAACTATTGTTGAAGAGAAGAGGAAACAGACGTCAGAGTCCCTTGTTGAGAGAGAGACAGCTGTGTCCAAAAAGAAAAATCCTTCCTCGAAAGAATCCTATAGGGAAAGAAATGCTTCTACAACTCCCAAACAGAAGAAGAAGCCATCAGAGCTCACTGTGGATGGAGATGAGGCTGGGTCTAAGAAGGGAAGTCCTTTGGAAGAATCATTAAGTGCAATTGCTTCAACTGTACCCAAACTGAAGCAGAAGTCGACTTCTGCCGTGAAACTGGGTAGTAACAAAAATAGGGAAAAGACGTTTTCCGGTATAGATATTTCAAGGATGGTGAAACGAAATAATTCACTGAAAAATGAACTCCAGGCTTCCATGTCTGAGAAAATCAAGACCTCACTGGATGAAGAGCCATTTTCTTCAATGGAGTCTGAGCAGGTGAAGCTCGGGAACCTGGATACACCTGATGCTAATACAGCTAGTGAGATACACAAGGAAATGCTACCACATACTGAATGTGGTGAGGATGGAAATCAGCATCCTAACCCCAGCAAGTCTCGTATGCACTGTGCTCATGCTAATGGTCAAGTCCAATTCAAAATTCCTGATGATGCAGGCAGAAGGTGCACCACGAGTAATGATGAGCCTCGTTCGAGTGTTACCAATCCTGGGTCTGGCAATGAGAAAGGGTGCAAGAGAGAGAACTCGGGAAAACCATATTCAAATGGGACGAAACAGAAACAGAAACTGAGAACAGAATTTGACAGAGTCCCACCGGCTTGCATTAAAGGGAAGCAAGATCCTATTTTTTCAAGAGGCTTTGATCTAGCGGGTCAAAATTCAGTATATGACCATATTAGGACATTCTCATCTACTTTTTACAGCCATATAGGTCATGTTGCCGACTCGTTATCTAGTACTAACGGATTACCTATGCCATCAGATGCAACTAAATTGGTTGGTCCAAGAGCACCCCAACCTGGTTACCTGGATAGGTGTATGAGTTTCGCTCCTGGTCCGCAGATGAACTATTCACTACAGAGTTCAGCTGGTTGGATCGATGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006325 GO:0006355 GO:0006950 GO:0006952 GO:0006996 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0009605 GO:0009607 GO:0009620 GO:0009791 GO:0009889 GO:0009893 GO:0009909 GO:0009911 GO:0009987 GO:0010228 GO:0010468 GO:0010556 GO:0010604 GO:0010638 GO:0016043 GO:0019219 GO:0019222 GO:0022414 GO:0023052 GO:0030154 GO:0031056 GO:0031058 GO:0031060 GO:0031062 GO:0031323 GO:0031325 GO:0031326 GO:0031399 GO:0031401 GO:0031440 GO:0031935 GO:0031937 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033043 GO:0033044 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0043954 GO:0044030 GO:0044424 GO:0044464 GO:0048366 GO:0048367 GO:0048468 GO:0048518 GO:0048522 GO:0048580 GO:0048582 GO:0048608 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0048869 GO:0050684 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051052 GO:0051094 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051276 GO:0051570 GO:0051574 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060968 GO:0061085 GO:0061087 GO:0061458 GO:0065007 GO:0070827 GO:0070828 GO:0070829 GO:0071840 GO:0080090 GO:0090436 GO:0097159 GO:0098542 GO:0099402 GO:0140110 GO:1900109 GO:1900111 GO:1900363 GO:1901363 GO:1902275 GO:1903311 GO:1903506 GO:1905269 GO:2000024 GO:2000026 GO:2000112 GO:2000241 GO:2000243 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNMT1-RFD

Protein Analysis

958

Amino Acids

107.46

Weight (kDa)

8.51

Isoelectric Point (pI)

50.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNMT1-RFD PF12047 11 - 132 4.5e-09 Cytosine specific DNA methyltransferase replication foci domain
PHD_NSD PF22908 273 - 342 5.1e-19 Histone-lysine N-methyltransferase NSD-like, PHD zinc finger
PHDvar_NSD PF23004 344 - 380 5.9e-06 Histone-lysine N-methyltransferase NSD-like, variant PHD zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000343)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48090 AT5G48090 AT5G48090 AT5G55390 AT5G55390 AT5G55390
fragaria_vesca FvH4_3g23690 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_4g08730 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160 FvH4_5g31160
malus_domestica MD03G1191000.v1.1 MD11G1207800.v1.1 MD11G1208000.v1.1
prunus_persica Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227500_v2.0.a1 Prupe.4G227600_v2.0.a1 Prupe.4G227700_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1 Prupe.4G227800_v2.0.a1
pyrus_communis pycom01g03400 pycom03g14420 pycom11g18100 pycom11g18140 pycom11g18150 pycom11g18160
rosa_chinensis RchiOBHm_Chr2g0145981 RchiOBHm_Chr4g0405241 RchiOBHm_Chr4g0405271 RchiOBHm_Chr5g0016201 RchiOBHm_Chr5g0041831 RchiOBHm_Chr5g0041871 RchiOBHm_Chr5g0041881 RchiOBHm_Chr5g0059241
rosa_laevigata RLG00000002117 RLG00000008861 RLG00000008862 RLG00000011000 RLG00000033216 RLG00000034097 RLG00000034101
rosa_multiflora Rmu_sc0000161.1_g000001 Rmu_sc0000861.1_g000040 Rmu_sc0007522.1_g000003 Rmu_sc0009443.1_g000015 Rmu_sc0022129.1_g000001 Rmu_sc0042315.1_g000001
rosa_roxburghii Rroxscaffold_1G00038660 Rroxscaffold_1G00038690 Rroxscaffold_5G00349560 Rroxscaffold_5G00349610 Rroxscaffold_6G00388370 Rroxscaffold_7G00188740
rosa_rugosa Rorug03G0199700 Rorug04G0055400 Rorug04G0055500 Rorug04G0055900 Rorug04G0056000 Rorug04G0056100 Rorug04G0056100 Rorug04G0056200 Rorug05G0195600 Rorug05G0195700 Rorug05G0195800 Rorug05G0195900 Rorug05G0196800 Rorug05G0385000
rosa_samantha Rh4AG129900 Rh4AG130100 Rh4BG124200 Rh4BG124400 Rh4BG124500 Rh4CG137200 Rh4CG137300 Rh4DG123500 Rh4DG123700 Rh5AG280800 Rh5AG281400 Rh5BG286700 Rh5BG287000 Rh5BG397800 Rh5CG318100 Rh5CG318600 Rh5CG421600 Rh5DG132300 Rh5DG132400 Rh5DG295400 Rh5DG295700 Rh7BG475300
rosa_wichuraiana Rw0G001330 Rw0G005730 Rw4G008670 Rw4G010510 Rw4G010540 Rw5G026450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2236
AasI GACNNNNNNGTC 2 cut(s) 1852, 1879
AatII GACGTC 1 cut(s) 1850
Acc36I ACCTGC 1 cut(s) 2236
AccB1I GGYRCC 2 cut(s) 696, 731
AccB7I CCANNNNNTGG 1 cut(s) 908
AccI GTMKAC 2 cut(s) 1677, 2069
AccIII TCCGGA 1 cut(s) 346
AciI CCGC 5 cut(s) 153, 482, 728, 2639, 2828
AclWI GGATC 6 cut(s) 263, 441, 745, 1547, 2603, 2873
AcoI YGGCCR 1 cut(s) 498
AcsI RAATTY 7 cut(s) 790, 1250, 1280, 1364, 2409, 2567, 2647
AcuI CTGAAG 5 cut(s) 405, 573, 993, 1740, 2078
AcyI GRCGYC 1 cut(s) 1847
AdeI CACNNNGTG 2 cut(s) 334, 932
AfaI GTAC 6 cut(s) 41, 1109, 1215, 1560, 2049, 2728
AfiI CCNNNNNNNGG 5 cut(s) 449, 908, 2478, 2483, 2789
AflII CTTAAG 1 cut(s) 1436
AhlI ACTAGT 1 cut(s) 1570
AjnI CCWGG 6 cut(s) 2175, 2262, 2476, 2788, 2796, 2821
AjuI GAANNNNNNNTTGG 4 cut(s) 1521, 1553, 2394, 2426
AloI GAACNNNNNNTCC 2 cut(s) 835, 867
Alw21I GWGCWC 7 cut(s) 321, 1310, 1747, 1971, 2383, 2438, 2782
Alw26I GTCTC 6 cut(s) 76, 106, 1374, 1675, 1865, 2370
Alw44I GTGCAC 2 cut(s) 317, 2434
AlwI GGATC 6 cut(s) 263, 441, 745, 1547, 2603, 2873
AlwNI CAGNNNCTG 2 cut(s) 875, 2557
Ama87I CYCGRG 2 cut(s) 2255, 2515
Aor13HI TCCGGA 1 cut(s) 346
AoxI GGCC 3 cut(s) 410, 440, 498
ApaLI GTGCAC 2 cut(s) 317, 2434
ApeKI GCWGC 2 cut(s) 875, 1032
ApoI RAATTY 7 cut(s) 790, 1250, 1280, 1364, 2409, 2567, 2647
Asp700I GAANNNNTTC 4 cut(s) 1405, 1711, 1930, 2155
AspS9I GGNCC 3 cut(s) 1270, 2771, 2825
AsuHPI GGTGA 7 cut(s) 119, 368, 464, 653, 2155, 2260, 2342
AvaI CYCGRG 2 cut(s) 2255, 2515
AvaII GGWCC 3 cut(s) 1270, 2771, 2825
AxyI CCTNAGG 2 cut(s) 701, 868
BaeGI GKGCMC 2 cut(s) 321, 2438
BalI TGGCCA 1 cut(s) 500
BanI GGYRCC 2 cut(s) 696, 731
BanII GRGCYC 1 cut(s) 1971
BauI CACGAG 1 cut(s) 2440
BbsI GAAGAC 4 cut(s) 524, 1334, 1475, 1634
Bbv12I GWGCWC 7 cut(s) 321, 1310, 1747, 1971, 2383, 2438, 2782
BbvCI CCTCAGC 1 cut(s) 879
BbvI GCAGC 2 cut(s) 862, 1019
BceAI ACGGC 1 cut(s) 2063
BciT130I CCWGG 6 cut(s) 2177, 2264, 2478, 2790, 2798, 2823
BciVI GTATCC 3 cut(s) 541, 907, 2260
BcoDI GTCTC 6 cut(s) 76, 106, 1374, 1675, 1865, 2370
BcuI ACTAGT 1 cut(s) 1570
BfaI CTAG 7 cut(s) 558, 1163, 1473, 1571, 2289, 2636, 2724
BfmI CTRYAG 4 cut(s) 1428, 1674, 1915, 2846
BfrI CTTAAG 1 cut(s) 1436
BfuAI ACCTGC 1 cut(s) 2236
BfuI GTATCC 3 cut(s) 541, 907, 2260
BisI GCNGC 2 cut(s) 876, 1033
BlsI GCNGC 2 cut(s) 877, 1034
BmcAI AGTACT 1 cut(s) 2728
Bme1390I CCNGG 6 cut(s) 2177, 2264, 2478, 2790, 2798, 2823
Bme18I GGWCC 3 cut(s) 1270, 2771, 2825
BmeT110I CYCGRG 2 cut(s) 2255, 2515
BmgT120I GGNCC 3 cut(s) 1270, 2771, 2825
BmiI GGNNCC 6 cut(s) 151, 698, 733, 853, 1271, 2261
BmrFI CCNGG 6 cut(s) 2177, 2264, 2478, 2790, 2798, 2823
BmrI ACTGGG 1 cut(s) 2096
BmsI GCATC 6 cut(s) 14, 774, 2266, 2356, 2410, 2743
BmuI ACTGGG 1 cut(s) 2096
BpiI GAAGAC 4 cut(s) 524, 1334, 1475, 1634
BplI GAGNNNNNCTC 4 cut(s) 1693, 1725, 2803, 2835
BpmI CTGGAG 1 cut(s) 2159
Bpu10I CCTNAGC 1 cut(s) 879
BpuEI CTTGAG 1 cut(s) 1001
Bsa29I ATCGAT 3 cut(s) 582, 627, 2868
BsaBI GATNNNNATC 1 cut(s) 2340
BsaHI GRCGYC 1 cut(s) 1847
BsaI GGTCTC 1 cut(s) 1675
BsaJI CCNNGG 4 cut(s) 272, 1127, 1410, 2477
BsaWI WCCGGW 2 cut(s) 346, 2120
BsaXI ACNNNNNCTCC 4 cut(s) 91, 121, 1371, 1401
Bsc4I CCNNNNNNNGG 5 cut(s) 449, 908, 2478, 2483, 2789
Bse118I RCCGGY 1 cut(s) 2584
Bse1I ACTGG 4 cut(s) 120, 826, 2091, 2214
Bse21I CCTNAGG 2 cut(s) 701, 868
Bse3DI GCAATG 1 cut(s) 2494
Bse8I GATNNNNATC 1 cut(s) 2340
BseAI TCCGGA 1 cut(s) 346
BseBI CCWGG 6 cut(s) 2177, 2264, 2478, 2790, 2798, 2823
BseCI ATCGAT 3 cut(s) 582, 627, 2868
BseDI CCNNGG 4 cut(s) 272, 1127, 1410, 2477
BseJI GATNNNNATC 1 cut(s) 2340
BseLI CCNNNNNNNGG 5 cut(s) 449, 908, 2478, 2483, 2789
BseMI GCAATG 1 cut(s) 2494
BseMII CTCAG 9 cut(s) 213, 252, 583, 711, 870, 882, 2181, 2232, 2549
BseNI ACTGG 4 cut(s) 120, 826, 2091, 2214
BseRI GAGGAG 2 cut(s) 737, 1717
BseSI GKGCMC 2 cut(s) 321, 2438
BseXI GCAGC 2 cut(s) 862, 1019
BseYI CCCAGC 2 cut(s) 1991, 2357
BshFI GGCC 3 cut(s) 412, 442, 500
BshNI GGYRCC 2 cut(s) 696, 731
BshVI ATCGAT 3 cut(s) 582, 627, 2868
BsiHKAI GWGCWC 7 cut(s) 321, 1310, 1747, 1971, 2383, 2438, 2782
BsiHKCI CYCGRG 2 cut(s) 2255, 2515
BsiSI CCGG 4 cut(s) 347, 735, 2121, 2585
BslFI GGGAC 4 cut(s) 1283, 1840, 2551, 2564
BslI CCNNNNNNNGG 5 cut(s) 449, 908, 2478, 2483, 2789
BsmAI GTCTC 6 cut(s) 76, 106, 1374, 1675, 1865, 2370
BsmFI GGGAC 4 cut(s) 1283, 1840, 2551, 2564
BsnI GGCC 3 cut(s) 412, 442, 500
Bso31I GGTCTC 1 cut(s) 1675
BsoBI CYCGRG 2 cut(s) 2255, 2515
Bsp1286I GDGCHC 7 cut(s) 321, 1310, 1747, 1971, 2383, 2438, 2782
Bsp13I TCCGGA 1 cut(s) 346
BspACI CCGC 5 cut(s) 153, 482, 728, 2639, 2828
BspANI GGCC 3 cut(s) 412, 442, 500
BspCNI CTCAG 9 cut(s) 214, 251, 582, 712, 871, 881, 2182, 2233, 2550
BspDI ATCGAT 3 cut(s) 582, 627, 2868
BspEI TCCGGA 1 cut(s) 346
BspLI GGNNCC 6 cut(s) 151, 698, 733, 853, 1271, 2261
BspMI ACCTGC 1 cut(s) 2236
BspPI GGATC 6 cut(s) 263, 441, 745, 1547, 2603, 2873
BspQI GCTCTTC 4 cut(s) 210, 272, 1505, 2211
BspT107I GGYRCC 2 cut(s) 696, 731
BspTI CTTAAG 1 cut(s) 1436
BspTNI GGTCTC 1 cut(s) 1675
BsrDI GCAATG 1 cut(s) 2494
BsrFI RCCGGY 1 cut(s) 2584
BsrI ACTGG 4 cut(s) 120, 826, 2091, 2214
BssAI RCCGGY 1 cut(s) 2584
BssECI CCNNGG 4 cut(s) 272, 1127, 1410, 2477
BssNI GRCGYC 1 cut(s) 1847
BssSI CACGAG 1 cut(s) 2440
BssT1I CCWWGG 2 cut(s) 1127, 1410
Bst2BI CACGAG 1 cut(s) 2440
Bst2UI CCWGG 6 cut(s) 2177, 2264, 2478, 2790, 2798, 2823
BstACI GRCGYC 1 cut(s) 1847
BstAFI CTTAAG 1 cut(s) 1436
BstAPI GCANNNNNTGC 1 cut(s) 2433
BstC8I GCNNGC 4 cut(s) 1091, 1124, 2425, 2590
BstEII GGTNACC 1 cut(s) 2792
BstMAI GTCTC 6 cut(s) 76, 106, 1374, 1675, 1865, 2370
BstMWI GCNNNNNNNGC 6 cut(s) 268, 1430, 1966, 2251, 2285, 2433
BstNI CCWGG 6 cut(s) 2177, 2264, 2478, 2790, 2798, 2823
BstNSI RCATGY 2 cut(s) 185, 1093
BstPI GGTNACC 1 cut(s) 2792
BstSCI CCNGG 6 cut(s) 2175, 2262, 2476, 2788, 2796, 2821
BstSFI CTRYAG 4 cut(s) 1428, 1674, 1915, 2846
BstSLI GKGCMC 2 cut(s) 321, 2438
BstV1I GCAGC 2 cut(s) 862, 1019
BstV2I GAAGAC 4 cut(s) 524, 1334, 1475, 1634
BstX2I RGATCY 2 cut(s) 737, 2608
BstXI CCANNNNNNTGG 2 cut(s) 496, 959
BstYI RGATCY 2 cut(s) 737, 2608
Bsu15I ATCGAT 3 cut(s) 582, 627, 2868
Bsu36I CCTNAGG 2 cut(s) 701, 868
BsuI GTATCC 3 cut(s) 541, 907, 2260
BsuRI GGCC 3 cut(s) 412, 442, 500
BsuTUI ATCGAT 3 cut(s) 582, 627, 2868
BtsI GCAGTG 1 cut(s) 319
BtsIMutI CAGTG 7 cut(s) 291, 319, 833, 1971, 2159, 2207, 2374
BveI ACCTGC 1 cut(s) 2236
Cac8I GCNNGC 4 cut(s) 1091, 1124, 2425, 2590
CaiI CAGNNNCTG 2 cut(s) 875, 2557
Cfr10I RCCGGY 1 cut(s) 2584
Cfr13I GGNCC 3 cut(s) 1270, 2771, 2825
ClaI ATCGAT 3 cut(s) 582, 627, 2868
CsiI ACCWGGT 1 cut(s) 2788
Csp6I GTAC 6 cut(s) 40, 1108, 1214, 1559, 2048, 2727
CviQI GTAC 6 cut(s) 40, 1108, 1214, 1559, 2048, 2727
DraIII CACNNNGTG 2 cut(s) 334, 932
DrdI GACNNNNNNGTC 2 cut(s) 1852, 1879
DseDI GACNNNNNNGTC 2 cut(s) 1852, 1879
EaeI YGGCCR 1 cut(s) 498
Ecl136II GAGCTC 1 cut(s) 1969
Eco130I CCWWGG 2 cut(s) 1127, 1410
Eco147I AGGCCT 2 cut(s) 412, 442
Eco24I GRGCYC 1 cut(s) 1971
Eco31I GGTCTC 1 cut(s) 1675
Eco32I GATATC 2 cut(s) 470, 625
Eco47I GGWCC 3 cut(s) 1270, 2771, 2825
Eco53kI GAGCTC 1 cut(s) 1969
Eco57I CTGAAG 5 cut(s) 405, 573, 993, 1740, 2078
Eco81I CCTNAGG 2 cut(s) 701, 868
Eco88I CYCGRG 2 cut(s) 2255, 2515
Eco91I GGTNACC 1 cut(s) 2792
EcoICRI GAGCTC 1 cut(s) 1969
EcoO65I GGTNACC 1 cut(s) 2792
EcoRI GAATTC 1 cut(s) 1364
EcoRII CCWGG 6 cut(s) 2175, 2262, 2476, 2788, 2796, 2821
EcoRV GATATC 2 cut(s) 470, 625
EcoT14I CCWWGG 2 cut(s) 1127, 1410
EcoT38I GRGCYC 1 cut(s) 1971
ErhI CCWWGG 2 cut(s) 1127, 1410
FalI AAGNNNNNCTT 2 cut(s) 1915, 1947
FaqI GGGAC 4 cut(s) 1283, 1840, 2551, 2564
FauI CCCGC 2 cut(s) 721, 2632
FauNDI CATATG 1 cut(s) 1453
FblI GTMKAC 2 cut(s) 1677, 2069
Fnu4HI GCNGC 2 cut(s) 876, 1033
FriOI GRGCYC 1 cut(s) 1971
Fsp4HI GCNGC 2 cut(s) 876, 1033
FspBI CTAG 7 cut(s) 558, 1163, 1473, 1571, 2289, 2636, 2724
GluI GCNGC 2 cut(s) 876, 1033
GsaI CCCAGC 2 cut(s) 1995, 2361
GsuI CTGGAG 1 cut(s) 2159
HaeIII GGCC 3 cut(s) 412, 442, 500
HapII CCGG 4 cut(s) 347, 735, 2121, 2585
Hin1I GRCGYC 1 cut(s) 1847
HincII GTYRAC 3 cut(s) 20, 463, 2070
HindII GTYRAC 3 cut(s) 20, 463, 2070
HindIII AAGCTT 2 cut(s) 260, 1583
HpaI GTTAAC 1 cut(s) 463
HpaII CCGG 4 cut(s) 347, 735, 2121, 2585
HphI GGTGA 7 cut(s) 119, 368, 464, 653, 2155, 2260, 2342
Hpy166II GTNNAC 9 cut(s) 20, 319, 463, 812, 1043, 1214, 1678, 2070, 2436
Hpy188III TCNNGA 9 cut(s) 307, 347, 357, 1460, 2200, 2257, 2414, 2517, 2622
Hpy8I GTNNAC 9 cut(s) 20, 319, 463, 812, 1043, 1214, 1678, 2070, 2436
HpyCH4IV ACGT 2 cut(s) 1847, 2114
HpyF10VI GCNNNNNNNGC 6 cut(s) 268, 1430, 1966, 2251, 2285, 2433
HpySE526I ACGT 2 cut(s) 1847, 2114
Hsp92I GRCGYC 1 cut(s) 1847
Kpn2I TCCGGA 1 cut(s) 346
KspAI GTTAAC 1 cut(s) 463
LguI GCTCTTC 4 cut(s) 210, 272, 1505, 2211
LmnI GCTCC 3 cut(s) 1429, 1750, 2824
Lsp1109I GCAGC 2 cut(s) 862, 1019
LweI GCATC 6 cut(s) 14, 774, 2266, 2356, 2410, 2743
MabI ACCWGGT 1 cut(s) 2788
MaeI CTAG 7 cut(s) 558, 1163, 1473, 1571, 2289, 2636, 2724
MaeII ACGT 2 cut(s) 1847, 2114
MaeIII GTNAC 6 cut(s) 659, 1071, 1390, 2093, 2467, 2792
MfeI CAATTG 1 cut(s) 2034
MflI RGATCY 2 cut(s) 737, 2608
MhlI GDGCHC 7 cut(s) 321, 1310, 1747, 1971, 2383, 2438, 2782
MlsI TGGCCA 1 cut(s) 500
MluNI TGGCCA 1 cut(s) 500
MlyI GAGTC 4 cut(s) 1862, 2246, 2586, 2708
MmeI TCCRAC 2 cut(s) 233, 2843
Mox20I TGGCCA 1 cut(s) 500
MroI TCCGGA 1 cut(s) 346
MroXI GAANNNNTTC 4 cut(s) 1405, 1711, 1930, 2155
MscI TGGCCA 1 cut(s) 500
MseI TTAA 5 cut(s) 462, 1437, 1596, 2027, 2595
MslI CAYNNNNRTG 1 cut(s) 2388
Msp20I TGGCCA 1 cut(s) 500
MspA1I CMGCKG 3 cut(s) 155, 1877, 2858
MspCI CTTAAG 1 cut(s) 1436
MspI CCGG 4 cut(s) 347, 735, 2121, 2585
MspR9I CCNGG 6 cut(s) 2177, 2264, 2478, 2790, 2798, 2823
MunI CAATTG 1 cut(s) 2034
MvaI CCWGG 6 cut(s) 2177, 2264, 2478, 2790, 2798, 2823
MwoI GCNNNNNNNGC 6 cut(s) 268, 1430, 1966, 2251, 2285, 2433
NdeI CATATG 1 cut(s) 1453
NlaIV GGNNCC 6 cut(s) 151, 698, 733, 853, 1271, 2261
NmeAIII GCCGAG 1 cut(s) 297
NmuCI GTSAC 2 cut(s) 659, 1390
NspI RCATGY 2 cut(s) 185, 1093
PaeI GCATGC 1 cut(s) 1093
PaqCI CACCTGC 1 cut(s) 2236
PceI AGGCCT 2 cut(s) 412, 442
PciSI GCTCTTC 4 cut(s) 210, 272, 1505, 2211
PdmI GAANNNNTTC 4 cut(s) 1405, 1711, 1930, 2155
PfeI GAWTC 8 cut(s) 373, 485, 604, 1004, 1517, 1532, 1910, 2021
PflFI GACNNNGTC 1 cut(s) 2576
PflMI CCANNNNNTGG 1 cut(s) 908
PkrI GCNGC 2 cut(s) 877, 1034
PleI GAGTC 4 cut(s) 1861, 2245, 2585, 2708
PpsI GAGTC 4 cut(s) 1861, 2245, 2585, 2708
Psp124BI GAGCTC 1 cut(s) 1971
Psp6I CCWGG 6 cut(s) 2175, 2262, 2476, 2788, 2796, 2821
PspEI GGTNACC 1 cut(s) 2792
PspFI CCCAGC 2 cut(s) 1991, 2357
PspGI CCWGG 6 cut(s) 2175, 2262, 2476, 2788, 2796, 2821
PspN4I GGNNCC 6 cut(s) 151, 698, 733, 853, 1271, 2261
PspPI GGNCC 3 cut(s) 1270, 2771, 2825
PsrI GAACNNNNNNTAC 2 cut(s) 75, 107
PstNI CAGNNNCTG 2 cut(s) 875, 2557
PsuI RGATCY 2 cut(s) 737, 2608
PsyI GACNNNGTC 1 cut(s) 2576
PvuII CAGCTG 2 cut(s) 1877, 2858
RsaI GTAC 6 cut(s) 41, 1109, 1215, 1560, 2049, 2728
RsaNI GTAC 6 cut(s) 40, 1108, 1214, 1559, 2048, 2727
RseI CAYNNNNRTG 1 cut(s) 2388
SacI GAGCTC 1 cut(s) 1971
SalI GTCGAC 1 cut(s) 2068
SapI GCTCTTC 4 cut(s) 210, 272, 1505, 2211
SaqAI TTAA 5 cut(s) 462, 1437, 1596, 2027, 2595
SatI GCNGC 2 cut(s) 876, 1033
Sau96I GGNCC 3 cut(s) 1270, 2771, 2825
ScaI AGTACT 1 cut(s) 2728
SchI GAGTC 4 cut(s) 1862, 2246, 2586, 2708
ScrFI CCNGG 6 cut(s) 2177, 2264, 2478, 2790, 2798, 2823
SduI GDGCHC 7 cut(s) 321, 1310, 1747, 1971, 2383, 2438, 2782
SexAI ACCWGGT 1 cut(s) 2788
SfaNI GCATC 6 cut(s) 14, 774, 2266, 2356, 2410, 2743
SfcI CTRYAG 4 cut(s) 1428, 1674, 1915, 2846
SinI GGWCC 3 cut(s) 1270, 2771, 2825
SmiMI CAYNNNNRTG 1 cut(s) 2388
SmlI CTYRAG 2 cut(s) 980, 1436
SmoI CTYRAG 2 cut(s) 980, 1436
SpeI ACTAGT 1 cut(s) 1570
SphI GCATGC 1 cut(s) 1093
SseBI AGGCCT 2 cut(s) 412, 442
SsiI CCGC 5 cut(s) 153, 482, 728, 2639, 2828
SspI AATATT 2 cut(s) 942, 1405
SspMI CTAG 7 cut(s) 558, 1163, 1473, 1571, 2289, 2636, 2724
SstI GAGCTC 1 cut(s) 1971
StuI AGGCCT 2 cut(s) 412, 442
StyD4I CCNGG 6 cut(s) 2175, 2262, 2476, 2788, 2796, 2821
StyI CCWWGG 2 cut(s) 1127, 1410
TaiI ACGT 2 cut(s) 1850, 2117
TatI WGTACW 3 cut(s) 1213, 1558, 2726
TfiI GAWTC 8 cut(s) 373, 485, 604, 1004, 1517, 1532, 1910, 2021
Tru1I TTAA 5 cut(s) 462, 1437, 1596, 2027, 2595
Tru9I TTAA 5 cut(s) 462, 1437, 1596, 2027, 2595
TscAI CASTG 7 cut(s) 298, 326, 833, 1978, 2166, 2214, 2381
TseFI GTSAC 2 cut(s) 659, 1390
TseI GCWGC 2 cut(s) 875, 1032
Tsp45I GTSAC 2 cut(s) 659, 1390
TspDTI ATGAA 9 cut(s) 68, 69, 539, 596, 701, 1013, 2184, 2229, 2849
TspGWI ACGGA 2 cut(s) 1392, 2748
TspRI CASTG 7 cut(s) 298, 326, 833, 1978, 2166, 2214, 2381
Tth111I GACNNNGTC 1 cut(s) 2576
Van91I CCANNNNNTGG 1 cut(s) 908
Vha464I CTTAAG 1 cut(s) 1436
VneI GTGCAC 2 cut(s) 317, 2434
VpaK11BI GGWCC 3 cut(s) 1270, 2771, 2825
XapI RAATTY 7 cut(s) 790, 1250, 1280, 1364, 2409, 2567, 2647
XceI RCATGY 2 cut(s) 185, 1093
XmiI GTMKAC 2 cut(s) 1677, 2069
XmnI GAANNNNTTC 4 cut(s) 1405, 1711, 1930, 2155
XspI CTAG 7 cut(s) 558, 1163, 1473, 1571, 2289, 2636, 2724
ZraI GACGTC 1 cut(s) 1848
ZrmI AGTACT 1 cut(s) 2728
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.