Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0000283

Physical Location & Seq

Genomic Coordinates Reverse (-)

21524018 .. 21527091

Gene Structure 3074 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1101900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2040 bp

ATGATCACAATGCTTTCCATCAATCTGAATGTGTTCATGACTCTTGTGCTGATCGGCTTCCTAAGCCTCAGCCGTACTAGTGAAGCTGCCCCAAATTACCGCTACCATGTCTGTGATAACACAACCAGTTTGGCTCCCAACAGCACCTACCAGTCAAACCTTAACCTCCTCCTCTCTTCTCTTACCTCCAATGCAACCATTGACACCGGATACTACTACACTACCGCCGGCTCTGAAGATTCCAACTCTGCAGTTTACGGTGCTTTTCTGTGTCGCGGCGATCTCACAGCCAGCAAATGCCAAGATTGTGTTACTGCTGCGGCCAGAGAAGCAGTTCAAATGTACTGCCCCTTAAGAAAAGTGACTCTGTTATGGTACGATGAGTGCATGTTACGCTACACAAACGTGTCTTTCTTCCGTAAACTGGATGAAAGCCCTAGGGGCTTTCTGTGGAACGTGGAGAATGCTAGTGAACCAAACCGGTTTAGGCAACTGGTGGCTGAGACTGTCACTGGCCTAGTGTCCGTAGCTGCAAACGCACCTTCCCGTGCTAAAAAACTTGCAACCAAAGAAGAAAACTTTACTGGGTTTGAGGAGCTGTATAGCCTTGCGCAGTGCACGCCTGACCTATCAAGCACCGATTGTGATCAGTGTCTTAGAGGAGCTATTGCACTTTTTCCGGCATGCTGTGACGGAAAGCTAGGAGGAAGAGTTTTGTTTCCTAGTTGTAATGTTAGATATGAGGTGTATCCATTTTATACACTTGTTGCTGCCGCACCTCCGCCCACACCAGTTTTTCTTCCTCCTCCACCTATTCAGCCTCCAGGCTCAGTGCCAAGATCTCATGGAAATGATGAACAAATTTCATCATGGATCGTAGTGGCGATTGTTGTTCCAATTGCACTATTGCTTTTCTTGGTAGGCTGCCTTTTCCATAGAAGAAGAGCGAAGAAAAAGTACAATGTGATACAAGAACAAAATGTGGGGACGGAAATAAATGCTGTCGAGTCATTGCAGTTTAGTTTTGCTACAATTGAAGCAGCGACAAACAAGTTCTCTGTTCATAACAAGCTAGGGAAGGGTGGCTTCGGTGAGGTCTACAAGGGCATATTTCCTGATGGGCAACAAGTGGCCGTGAAGAGGTTGTCTAGGGGATCCGGGCAAGGCGCTGAGGAGTTTAAGAATGAGGTTGTTTTGGTAGCTAAGCTTCAGCACAGAAACTTAGTGAGGCTACTAGGATTTTGCTCCGAAGGTCCAGAAAAGATACTTGTTTACGAATTTGTGCAGAACAAAAGCCTTGACCACTTTCTATATGACTCCGGGGATCAAGTGAAATTGGACTGGCCAAGCAGATACAAGATTATCAACGGGATTGCTCGAGGGATTCTCTATCTCCATCATGATTCACGGCTTAAAATCATACATCGTGATCTTAAAGCGAGTAATGTATTGTTAGATGGGGAAATGAACCCAAAAATTGCCGATTTTGGTATGGCAAGAATATTTAGAGTTGATCAAACTCAAGGAAACACAAGAAGGGTTGTGGGAACGTATGGTTATATTGCCCCAGAATATGCGATGCATGGACTGTTCTCTGTGAAGTCTGATGTGTACAGTCTTGGTGTCTTGATCTTAGAGATTGTAACTGGCAAGAAGAACACTAATTTCTATAACTCAGACAACGGCGAAGACCTCTTGAGCCATGTTTGGAGACATTGGAGGGACGGGACACCGTTAGAATTGCTGGACGTAAATCTAAGAGATTCATATTCAAGAAGTGAAGTAATTAGATGTATCCATATTGGGTTACTCTGTGTTCAGGAAAATCCAGATGATAGACCGAAAATGCAAAAGATACTTCTCATGCTAAGCAGTTACTCTTTGAGTATGCCATCACCTCAAAAGCCAGCATATTTCCTGCACAGTAGGACAGATCCGAACATGCCAGCAATGAATTGTAGCTCATCTGATCAACCTGCTAGCAACTCATTGTCATTATCTGTTAATGAAGCATCGATTACTGAACTATACCCTCGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0042742 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050896 GO:0051704 GO:0051707 GO:0071704 GO:0071944 GO:0098542 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr Stress-antifung

Protein Analysis

680

Amino Acids

75.68

Weight (kDa)

7.8

Isoelectric Point (pI)

41.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Stress-antifung	PF01657	38 - 135	1.8e-23	Salt stress response/antifungal
Stress-antifung	PF01657	156 - 249	2.7e-10	Salt stress response/antifungal
Pkinase	PF00069	352 - 620	1.4e-44	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	354 - 620	6.6e-47	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000283)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G45860 AT4G05200 AT4G05200 AT4G11521 AT4G11530 AT4G23130 AT4G23130 AT4G23130 AT4G23140 AT4G23140 AT4G23150 AT4G23160 AT4G23170 AT4G23180 AT4G23180 AT4G23180 AT4G23230 AT4G23270 AT4G23270 AT4G23270 AT4G23280 AT4G23280 AT4G23280 AT4G23310 AT4G23310
fragaria_vesca	FvH4_3g02772 FvH4_3g02772 FvH4_3g02773 FvH4_3g02773 FvH4_3g02774 FvH4_3g02800 FvH4_3g02820 FvH4_3g02820 FvH4_3g02830
malus_domestica	MD00G1101900.v1.1 MD00G1102100.v1.1 MD00G1102200.v1.1 MD01G1098200.v1.1 MD05G1340000.v1.1 MD05G1340300.v1.1 MD07G1014100.v1.1 MD07G1014300.v1.1 MD07G1014400.v1.1 MD07G1014700.v1.1 MD10G1312900.v1.1 MD11G1022600.v1.1 MD11G1291500.v1.1 MD14G1207100.v1.1 MD16G1096600.v1.1
prunus_persica	Prupe.4G027300_v2.0.a1 Prupe.4G027400_v2.0.a1 Prupe.4G027700_v2.0.a1 Prupe.4G027800_v2.0.a1
pyrus_communis	pycom05g30960 pycom10g00940 pycom10g00950 pycom10g26430 pycom10g26450 pycom10g26460 pycom10g26470 pycom10g26480 pycom10g26490 pycom10g26500 pycom10g26510 pycom10g26570 pycom11g01820 pycom11g04000 pycom14g17130 pycom14g17140 pycom15g28820
rosa_chinensis	RchiOBHm_Chr4g0408141 RchiOBHm_Chr5g0004211 RchiOBHm_Chr5g0004231 RchiOBHm_Chr5g0004241 RchiOBHm_Chr5g0004261
rosa_laevigata	RLG00000008668 RLG00000008672 RLG00000031212 RLG00000031217
rosa_multiflora	Rmu_co8162658.1_g000001 Rmu_co8336297.1_g000001 Rmu_sc0002073.1_g000001 Rmu_sc0004964.1_g000002 Rmu_sc0004964.1_g000003 Rmu_sc0004964.1_g000007 Rmu_sc0006006.1_g000007 Rmu_sc0007868.1_g000015 Rmu_sc0007868.1_g000016 Rmu_sc0007868.1_g000017 Rmu_sc0007868.1_g000021 Rmu_sc0007868.1_g000023 Rmu_sc0019654.1_g000002 Rmu_sc0038540.1_g000001
rosa_roxburghii	Rroxscaffold_1G00071450 Rroxscaffold_1G00071460 Rroxscaffold_1G00071470 Rroxscaffold_1G00071560 Rroxscaffold_1G00071720 Rroxscaffold_1G00071770 Rroxscaffold_5G00352640
rosa_rugosa	Rorug04G0083700 Rorug04G0410700 Rorug04G0410800 Rorug04G0410900
rosa_samantha	Rh4BG145400 Rh4CG154900 Rh4DG141900 Rh5BG037500 Rh5BG037600 Rh5BG037700 Rh5CG041000 Rh5CG041100 Rh5CG041200 Rh5CG041300 Rh5CG041400 Rh5DG037100
rosa_wichuraiana	Rw4G012130 Rw5G003450 Rw5G003460 Rw5G003470 Rw5G003480 Rw5G003640 Rw5G003660 Rw5G003670 Rw5G003680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	612
Acc36I	ACCTGC	1 cut(s)	1984
AccI	GTMKAC	1 cut(s)	1098
AccII	CGCG	1 cut(s)	276
AciI	CCGC	6 cut(s)	100, 225, 276, 320, 774, 782
AclWI	GGATC	5 cut(s)	881, 1149, 1162, 1332, 1928
AcoI	YGGCCR	3 cut(s)	321, 1131, 1343
AcsI	RAATTY	2 cut(s)	861, 1277
AcuI	CTGAAG	2 cut(s)	255, 1193
AfaI	GTAC	5 cut(s)	76, 344, 377, 959, 1613
AfiI	CCNNNNNNNGG	2 cut(s)	424, 1140
AflII	CTTAAG	1 cut(s)	352
AflIII	ACRYGT	1 cut(s)	405
AgeI	ACCGGT	1 cut(s)	480
AgsI	TTSAA	3 cut(s)	338, 1037, 1773
AhlI	ACTAGT	1 cut(s)	77
AjnI	CCWGG	1 cut(s)	823
AleI	CACNNNNGTG	1 cut(s)	404
AluBI	AGCT	9 cut(s)	86, 530, 598, 665, 700, 1072, 1202, 1207, 1962
AluI	AGCT	9 cut(s)	86, 530, 598, 665, 700, 1072, 1202, 1207, 1962
Alw21I	GWGCWC	1 cut(s)	620
Alw26I	GTCTC	2 cut(s)	497, 1705
Alw44I	GTGCAC	1 cut(s)	616
AlwI	GGATC	5 cut(s)	881, 1149, 1162, 1332, 1928
Ama87I	CYCGRG	1 cut(s)	1377
AoxI	GGCC	4 cut(s)	321, 514, 1131, 1343
ApaLI	GTGCAC	1 cut(s)	616
ApeKI	GCWGC	6 cut(s)	86, 317, 530, 770, 924, 1040
ApoI	RAATTY	2 cut(s)	861, 1277
AsiGI	ACCGGT	1 cut(s)	480
Asp700I	GAANNNNTTC	2 cut(s)	32, 333
AspA2I	CCTAGG	1 cut(s)	437
AspLEI	GCGC	2 cut(s)	613, 1169
AspS9I	GGNCC	1 cut(s)	1253
AsuC2I	CCSGG	2 cut(s)	1159, 1321
AsuHPI	GGTGA	2 cut(s)	1103, 1887
AsuNHI	GCTAGC	1 cut(s)	1979
AvaI	CYCGRG	1 cut(s)	1377
AvaII	GGWCC	1 cut(s)	1253
AvrII	CCTAGG	1 cut(s)	437
BaeGI	GKGCMC	1 cut(s)	620
BalI	TGGCCA	1 cut(s)	1345
BamHI	GGATCC	1 cut(s)	1154
BarI	GAAGNNNNNNTAC	4 cut(s)	941, 973, 1191, 1223
BbsI	GAAGAC	1 cut(s)	1695
Bbv12I	GWGCWC	1 cut(s)	620
BbvCI	CCTCAGC	2 cut(s)	68, 1170
BbvI	GCAGC	6 cut(s)	73, 304, 517, 757, 911, 1052
BccI	CCATC	5 cut(s)	26, 1112, 1404, 1451, 1900
BceAI	ACGGC	4 cut(s)	57, 1118, 1424, 1699
BciT130I	CCWGG	1 cut(s)	825
BciVI	GTATCC	3 cut(s)	203, 759, 1805
BclI	TGATCA	4 cut(s)	3, 646, 1513, 1969
BcnI	CCSGG	2 cut(s)	1159, 1321
BcoDI	GTCTC	2 cut(s)	497, 1705
BcuI	ACTAGT	1 cut(s)	77
BfmI	CTRYAG	1 cut(s)	249
BfoI	RGCGCY	1 cut(s)	1170
BfrI	CTTAAG	1 cut(s)	352
BfuAI	ACCTGC	1 cut(s)	1984
BfuI	GTATCC	3 cut(s)	203, 759, 1805
BglI	GCCNNNNNGGC	1 cut(s)	441
BglII	AGATCT	1 cut(s)	839
BisI	GCNGC	9 cut(s)	87, 277, 318, 321, 531, 771, 774, 925, 1041
BlnI	CCTAGG	1 cut(s)	437
BlpI	GCTNAGC	2 cut(s)	1203, 1868
BlsI	GCNGC	9 cut(s)	88, 278, 319, 322, 532, 772, 775, 926, 1042
Bme1390I	CCNGG	3 cut(s)	825, 1159, 1321
Bme18I	GGWCC	1 cut(s)	1253
BmeT110I	CYCGRG	1 cut(s)	1377
BmgT120I	GGNCC	1 cut(s)	1253
BmiI	GGNNCC	2 cut(s)	135, 1156
BmrFI	CCNGG	3 cut(s)	825, 1159, 1321
BmrI	ACTGGG	1 cut(s)	594
BmsI	GCATC	2 cut(s)	1569, 2021
BmtI	GCTAGC	1 cut(s)	1983
BmuI	ACTGGG	1 cut(s)	594
BpiI	GAAGAC	1 cut(s)	1695
BplI	GAGNNNNNCTC	2 cut(s)	1371, 1403
BpmI	CTGGAG	1 cut(s)	807
Bpu10I	CCTNAGC	3 cut(s)	62, 68, 1170
Bpu1102I	GCTNAGC	2 cut(s)	1203, 1868
BpuEI	CTTGAG	2 cut(s)	1506, 1717
BpuMI	CCSGG	2 cut(s)	1159, 1321
Bsa29I	ATCGAT	1 cut(s)	2015
BsaBI	GATNNNNATC	1 cut(s)	645
BsaJI	CCNNGG	2 cut(s)	437, 1320
BsaWI	WCCGGW	2 cut(s)	206, 480
BsaXI	ACNNNNNCTCC	2 cut(s)	696, 726
Bsc4I	CCNNNNNNNGG	2 cut(s)	424, 1140
Bse118I	RCCGGY	2 cut(s)	227, 480
Bse1I	ACTGG	9 cut(s)	126, 151, 429, 498, 517, 589, 791, 1346, 1651
Bse3DI	GCAATG	2 cut(s)	1010, 1956
Bse8I	GATNNNNATC	1 cut(s)	645
BseBI	CCWGG	1 cut(s)	825
BseCI	ATCGAT	1 cut(s)	2015
BseDI	CCNNGG	2 cut(s)	437, 1320
BseGI	GGATG	1 cut(s)	433
BseJI	GATNNNNATC	1 cut(s)	645
BseLI	CCNNNNNNNGG	2 cut(s)	424, 1140
BseMI	GCAATG	2 cut(s)	1010, 1956
BseMII	CTCAG	5 cut(s)	82, 492, 843, 1161, 1689
BseNI	ACTGG	9 cut(s)	126, 151, 429, 498, 517, 589, 791, 1346, 1651
BseRI	GAGGAG	6 cut(s)	158, 161, 608, 675, 795, 1187
BseSI	GKGCMC	1 cut(s)	620
BseXI	GCAGC	6 cut(s)	73, 304, 517, 757, 911, 1052
BsgI	GTGCAG	2 cut(s)	1304, 1904
Bsh1236I	CGCG	1 cut(s)	276
BshFI	GGCC	4 cut(s)	323, 516, 1133, 1345
BshTI	ACCGGT	1 cut(s)	480
BshVI	ATCGAT	1 cut(s)	2015
BsiHKAI	GWGCWC	1 cut(s)	620
BsiHKCI	CYCGRG	1 cut(s)	1377
BsiSI	CCGG	6 cut(s)	207, 228, 481, 680, 1158, 1320
BslFI	GGGAC	3 cut(s)	1000, 1736, 1741
BslI	CCNNNNNNNGG	2 cut(s)	424, 1140
BsmAI	GTCTC	2 cut(s)	497, 1705
BsmFI	GGGAC	3 cut(s)	1000, 1736, 1741
BsmI	GAATGC	1 cut(s)	469
BsnI	GGCC	4 cut(s)	323, 516, 1133, 1345
BsoBI	CYCGRG	1 cut(s)	1377
Bsp1286I	GDGCHC	1 cut(s)	620
Bsp1407I	TGTACA	1 cut(s)	1611
Bsp1720I	GCTNAGC	2 cut(s)	1203, 1868
BspACI	CCGC	6 cut(s)	100, 225, 276, 320, 774, 782
BspANI	GGCC	4 cut(s)	323, 516, 1133, 1345
BspCNI	CTCAG	5 cut(s)	81, 493, 842, 1162, 1688
BspDI	ATCGAT	1 cut(s)	2015
BspFNI	CGCG	1 cut(s)	276
BspHI	TCATGA	2 cut(s)	36, 1399
BspLI	GGNNCC	2 cut(s)	135, 1156
BspMAI	CTGCAG	1 cut(s)	253
BspMI	ACCTGC	1 cut(s)	1984
BspOI	GCTAGC	1 cut(s)	1983
BspPI	GGATC	5 cut(s)	881, 1149, 1162, 1332, 1928
BspQI	GCTCTTC	1 cut(s)	937
BspTI	CTTAAG	1 cut(s)	352
BsrDI	GCAATG	2 cut(s)	1010, 1956
BsrFI	RCCGGY	2 cut(s)	227, 480
BsrGI	TGTACA	1 cut(s)	1611
BsrI	ACTGG	9 cut(s)	126, 151, 429, 498, 517, 589, 791, 1346, 1651
BssAI	RCCGGY	2 cut(s)	227, 480
BssECI	CCNNGG	2 cut(s)	437, 1320
BssT1I	CCWWGG	1 cut(s)	437
Bst2UI	CCWGG	1 cut(s)	825
Bst4CI	ACNGT	6 cut(s)	260, 508, 1590, 1616, 1734, 1925
Bst6I	CTCTTC	4 cut(s)	181, 703, 937, 1133
BstAFI	CTTAAG	1 cut(s)	352
BstAUI	TGTACA	1 cut(s)	1611
BstC8I	GCNNGC	7 cut(s)	229, 292, 620, 685, 1908, 1947, 1981
BstF5I	GGATG	1 cut(s)	433
BstFNI	CGCG	1 cut(s)	276
BstH2I	RGCGCY	1 cut(s)	1170
BstHHI	GCGC	2 cut(s)	613, 1169
BstMAI	GTCTC	2 cut(s)	497, 1705
BstMWI	GCNNNNNNNGC	6 cut(s)	63, 329, 393, 441, 536, 619
BstNI	CCWGG	1 cut(s)	825
BstNSI	RCATGY	3 cut(s)	391, 687, 1945
BstSCI	CCNGG	3 cut(s)	823, 1157, 1319
BstSFI	CTRYAG	1 cut(s)	249
BstSLI	GKGCMC	1 cut(s)	620
BstUI	CGCG	1 cut(s)	276
BstV1I	GCAGC	6 cut(s)	73, 304, 517, 757, 911, 1052
BstV2I	GAAGAC	1 cut(s)	1695
BstX2I	RGATCY	3 cut(s)	839, 1154, 1933
BstYI	RGATCY	3 cut(s)	839, 1154, 1933
Bsu15I	ATCGAT	1 cut(s)	2015
BsuI	GTATCC	3 cut(s)	203, 759, 1805
BsuRI	GGCC	4 cut(s)	323, 516, 1133, 1345
BsuTUI	ATCGAT	1 cut(s)	2015
BtgZI	GCGATG	1 cut(s)	1592
BtsCI	GGATG	1 cut(s)	433
BtsI	GCAGTG	1 cut(s)	620
BtsIMutI	CAGTG	4 cut(s)	510, 620, 656, 837
BveI	ACCTGC	1 cut(s)	1984
Cac8I	GCNNGC	7 cut(s)	229, 292, 620, 685, 1908, 1947, 1981
CciI	TCATGA	2 cut(s)	36, 1399
CfoI	GCGC	2 cut(s)	613, 1169
Cfr10I	RCCGGY	2 cut(s)	227, 480
Cfr13I	GGNCC	1 cut(s)	1253
ClaI	ATCGAT	1 cut(s)	2015
Csp6I	GTAC	5 cut(s)	75, 343, 376, 958, 1612
CspAI	ACCGGT	1 cut(s)	480
CviQI	GTAC	5 cut(s)	75, 343, 376, 958, 1612
EaeI	YGGCCR	3 cut(s)	321, 1131, 1343
Eam1104I	CTCTTC	4 cut(s)	181, 703, 937, 1133
EarI	CTCTTC	4 cut(s)	181, 703, 937, 1133
EciI	GGCGGA	1 cut(s)	771
Eco130I	CCWWGG	1 cut(s)	437
Eco47I	GGWCC	1 cut(s)	1253
Eco57I	CTGAAG	2 cut(s)	255, 1193
Eco88I	CYCGRG	1 cut(s)	1377
EcoRII	CCWGG	1 cut(s)	823
EcoT14I	CCWWGG	1 cut(s)	437
EcoT22I	ATGCAT	1 cut(s)	1584
ErhI	CCWWGG	1 cut(s)	437
FalI	AAGNNNNNCTT	2 cut(s)	1070, 1102
FaqI	GGGAC	3 cut(s)	1000, 1736, 1741
FbaI	TGATCA	4 cut(s)	3, 646, 1513, 1969
FblI	GTMKAC	1 cut(s)	1098
Fnu4HI	GCNGC	9 cut(s)	87, 277, 318, 321, 531, 771, 774, 925, 1041
FokI	GGATG	1 cut(s)	440
Fsp4HI	GCNGC	9 cut(s)	87, 277, 318, 321, 531, 771, 774, 925, 1041
FspI	TGCGCA	1 cut(s)	612
GlaI	GCGC	2 cut(s)	612, 1168
GluI	GCNGC	9 cut(s)	87, 277, 318, 321, 531, 771, 774, 925, 1041
GsuI	CTGGAG	1 cut(s)	807
HaeII	RGCGCY	1 cut(s)	1170
HaeIII	GGCC	4 cut(s)	323, 516, 1133, 1345
HapII	CCGG	6 cut(s)	207, 228, 481, 680, 1158, 1320
HhaI	GCGC	2 cut(s)	613, 1169
Hin6I	GCGC	2 cut(s)	611, 1167
HinP1I	GCGC	2 cut(s)	611, 1167
HindIII	AAGCTT	1 cut(s)	1205
HinfI	GANTC	8 cut(s)	40, 239, 364, 1007, 1316, 1384, 1403, 1763
HpaII	CCGG	6 cut(s)	207, 228, 481, 680, 1158, 1320
HphI	GGTGA	2 cut(s)	1103, 1887
Hpy166II	GTNNAC	7 cut(s)	256, 422, 473, 618, 1099, 1273, 1612
Hpy188I	TCNGA	7 cut(s)	27, 235, 1249, 1606, 1678, 1938, 1969
Hpy8I	GTNNAC	7 cut(s)	256, 422, 473, 618, 1099, 1273, 1612
HpyAV	CCTTC	4 cut(s)	552, 1072, 1244, 1530
HpyCH4III	ACNGT	6 cut(s)	260, 508, 1590, 1616, 1734, 1925
HpyCH4IV	ACGT	4 cut(s)	405, 456, 1550, 1749
HpyF10VI	GCNNNNNNNGC	6 cut(s)	63, 329, 393, 441, 536, 619
HpySE526I	ACGT	4 cut(s)	405, 456, 1550, 1749
HspAI	GCGC	2 cut(s)	611, 1167
KroI	GCCGGC	1 cut(s)	227
KroNI	GCCGGC	1 cut(s)	229
Ksp22I	TGATCA	4 cut(s)	3, 646, 1513, 1969
LguI	GCTCTTC	1 cut(s)	937
LmnI	GCTCC	4 cut(s)	139, 595, 662, 1250
Lsp1109I	GCAGC	6 cut(s)	73, 304, 517, 757, 911, 1052
LweI	GCATC	2 cut(s)	1569, 2021
MaeII	ACGT	4 cut(s)	405, 456, 1550, 1749
MaeIII	GTNAC	8 cut(s)	310, 361, 390, 508, 689, 1642, 1806, 1874
MfeI	CAATTG	2 cut(s)	897, 1032
MflI	RGATCY	3 cut(s)	839, 1154, 1933
MhlI	GDGCHC	1 cut(s)	620
MlsI	TGGCCA	1 cut(s)	1345
MluNI	TGGCCA	1 cut(s)	1345
MlyI	GAGTC	4 cut(s)	34, 358, 1016, 1310
MmeI	TCCRAC	1 cut(s)	267
Mox20I	TGGCCA	1 cut(s)	1345
Mph1103I	ATGCAT	1 cut(s)	1584
MroNI	GCCGGC	1 cut(s)	227
MroXI	GAANNNNTTC	2 cut(s)	32, 333
MscI	TGGCCA	1 cut(s)	1345
MseI	TTAA	6 cut(s)	162, 353, 1179, 1413, 1434, 2004
MslI	CAYNNNNRTG	3 cut(s)	111, 404, 849
Msp20I	TGGCCA	1 cut(s)	1345
MspCI	CTTAAG	1 cut(s)	352
MspI	CCGG	6 cut(s)	207, 228, 481, 680, 1158, 1320
MspR9I	CCNGG	3 cut(s)	825, 1159, 1321
MunI	CAATTG	2 cut(s)	897, 1032
Mva1269I	GAATGC	1 cut(s)	469
MvaI	CCWGG	1 cut(s)	825
MvnI	CGCG	1 cut(s)	276
MwoI	GCNNNNNNNGC	6 cut(s)	63, 329, 393, 441, 536, 619
NaeI	GCCGGC	1 cut(s)	229
NciI	CCSGG	2 cut(s)	1159, 1321
NgoMIV	GCCGGC	1 cut(s)	227
NheI	GCTAGC	1 cut(s)	1979
NlaIV	GGNNCC	2 cut(s)	135, 1156
NmuCI	GTSAC	3 cut(s)	361, 508, 689
NsbI	TGCGCA	1 cut(s)	612
NsiI	ATGCAT	1 cut(s)	1584
NspI	RCATGY	3 cut(s)	391, 687, 1945
OliI	CACNNNNGTG	1 cut(s)	404
PaeI	GCATGC	1 cut(s)	687
PaeR7I	CTCGAG	1 cut(s)	1377
PagI	TCATGA	2 cut(s)	36, 1399
PciSI	GCTCTTC	1 cut(s)	937
PctI	GAATGC	1 cut(s)	469
PdiI	GCCGGC	1 cut(s)	229
PdmI	GAANNNNTTC	2 cut(s)	32, 333
PfeI	GAWTC	4 cut(s)	239, 1384, 1403, 1763
PinAI	ACCGGT	1 cut(s)	480
PkrI	GCNGC	9 cut(s)	88, 278, 319, 322, 532, 772, 775, 926, 1042
PleI	GAGTC	4 cut(s)	34, 358, 1015, 1310
PpsI	GAGTC	4 cut(s)	34, 358, 1015, 1310
Psp6I	CCWGG	1 cut(s)	823
PspGI	CCWGG	1 cut(s)	823
PspN4I	GGNNCC	2 cut(s)	135, 1156
PspPI	GGNCC	1 cut(s)	1253
PspXI	VCTCGAGB	1 cut(s)	1377
PstI	CTGCAG	1 cut(s)	253
PsuI	RGATCY	3 cut(s)	839, 1154, 1933
RsaI	GTAC	5 cut(s)	76, 344, 377, 959, 1613
RsaNI	GTAC	5 cut(s)	75, 343, 376, 958, 1612
RseI	CAYNNNNRTG	3 cut(s)	111, 404, 849
SapI	GCTCTTC	1 cut(s)	937
SaqAI	TTAA	6 cut(s)	162, 353, 1179, 1413, 1434, 2004
SatI	GCNGC	9 cut(s)	87, 277, 318, 321, 531, 771, 774, 925, 1041
Sau96I	GGNCC	1 cut(s)	1253
SchI	GAGTC	4 cut(s)	34, 358, 1016, 1310
ScrFI	CCNGG	3 cut(s)	825, 1159, 1321
SduI	GDGCHC	1 cut(s)	620
SfaNI	GCATC	2 cut(s)	1569, 2021
SfcI	CTRYAG	1 cut(s)	249
Sfr274I	CTCGAG	1 cut(s)	1377
SinI	GGWCC	1 cut(s)	1253
SlaI	CTCGAG	1 cut(s)	1377
SmiMI	CAYNNNNRTG	3 cut(s)	111, 404, 849
SmlI	CTYRAG	4 cut(s)	352, 1377, 1521, 1696
SmoI	CTYRAG	4 cut(s)	352, 1377, 1521, 1696
SpeI	ACTAGT	1 cut(s)	77
SphI	GCATGC	1 cut(s)	687
SsiI	CCGC	6 cut(s)	100, 225, 276, 320, 774, 782
SspI	AATATT	1 cut(s)	1503
StyD4I	CCNGG	3 cut(s)	823, 1157, 1319
StyI	CCWWGG	1 cut(s)	437
TaaI	ACNGT	6 cut(s)	260, 508, 1590, 1616, 1734, 1925
TaiI	ACGT	4 cut(s)	408, 459, 1553, 1752
TaqI	TCGA	4 cut(s)	1005, 1378, 2015, 2035
TaqII	GACCGA	1 cut(s)	1855
TatI	WGTACW	3 cut(s)	342, 957, 1611
TauI	GCSGC	3 cut(s)	279, 323, 776
TfiI	GAWTC	4 cut(s)	239, 1384, 1403, 1763
Tru1I	TTAA	6 cut(s)	162, 353, 1179, 1413, 1434, 2004
Tru9I	TTAA	6 cut(s)	162, 353, 1179, 1413, 1434, 2004
TscAI	CASTG	4 cut(s)	517, 620, 656, 837
TseFI	GTSAC	3 cut(s)	361, 508, 689
TseI	GCWGC	6 cut(s)	86, 317, 530, 770, 924, 1040
Tsp45I	GTSAC	3 cut(s)	361, 508, 689
TspDTI	ATGAA	9 cut(s)	25, 444, 855, 870, 1052, 1481, 1755, 1967, 2022
TspGWI	ACGGA	4 cut(s)	407, 514, 708, 1004
TspRI	CASTG	4 cut(s)	517, 620, 656, 837
Vha464I	CTTAAG	1 cut(s)	352
VneI	GTGCAC	1 cut(s)	616
VpaK11BI	GGWCC	1 cut(s)	1253
XapI	RAATTY	2 cut(s)	861, 1277
XceI	RCATGY	3 cut(s)	391, 687, 1945
XhoI	CTCGAG	1 cut(s)	1377
XmaJI	CCTAGG	1 cut(s)	437
XmiI	GTMKAC	1 cut(s)	1098
XmnI	GAANNNNTTC	2 cut(s)	32, 333
Zsp2I	ATGCAT	1 cut(s)	1584

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.