Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0000283

Physical Location & Seq

Genomic Coordinates Reverse (-)

92884770 .. 92892735

Gene Structure 7966 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00071770.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3831 bp

ATGGCAGAAGTGATCCTTCTCGTCCTTTCAGTGGTCACCCTGCTAAGCTTTCCGACTACGGAAGCCGACTACCTCGCCCATATATGCCCCAACACCGCCGTCTTCACCCCAAACTCCACCTTCCAGTCCAACCTCAACCACCTCCTCTCCACTCTCTCCTCCAACGCCACACGCGACACCGGCTTCTACAACGCCACCTCCGGCCGTCTCCCTAACGACGCCGCCTACGGCCTCTTCCTCTGCCGCGGTGACGTCACCACCGCTGGTTGCAGAGCCTGCGTGACCACCGCAACCTCCGAGGTCGTGCGGCGCTGCCCGGTCGAGACAGAGGTTGTGATATGGTACGATGACTGCATGCTACGCTACTCCAACAGGTCCTTCTTCTCTGTCCCCGCCGAGTCGCCGATGTTGTTCATGTGGAACCCCCAAAACGTCAGCAGCGAAACGAACCGGTTCAACCAGGTTTTGGCGGCTAGCATGACGGAGGTGGCCACGGAGGCTTCGGGCGACGGCGATAAGTTCGCGACGAGAGAGGCGAACTTCACGGGGTTGATTAGCTTGTACAGCTTGGGGCAGTGCACGCAGGACTTGTCTACTTCTGATTGCAAACGGTGTCTTACGGGAGCCATTGCGAATTTGCCGAGTTGCTGCGGCGGGAAGTTAGGGGGACGAGTTCTGTATCCGAGTTGTAATGTTAGGTACGAAGTTTATCCCTTTTATAACTCGACGTCAGCACCGGCGCCGGCGCCAGGGGCTCGACCTCTTTCTCCGCCGAGCACAGTGACGAAAGGGAAGAGCAAAATCTCCTGGAAAAAGATTGTGGCCATTGCCGTTCCAATTGCAGCCTCTTTGGTGCTTTTTGTGATCTGCTACTGCCTCATTACTAAGAGGGCAAAAAAGAAGTACAATGCAGTAGCAGAAACAAGTGTGAATGACATTTCTAGTGTAGAGTCTTTGCAATATGATTTTGGTACCATTGAAGCTGCTACAAACAATTTCTCTGATGATAACAAGTTAGGCGAAGGTGGATTTGGTCAAGTCTACAAGGGTTTGCTTTCTGATGGTCAAGAAATAGCTGTCAAGAGGCTATCAAGGAACTCAGGCCAAGGTGCACAAGAATTTAAGAATGAGATGGTACTGGTAGCCAAGCTTCAACACCGAAATCTGGTCAGGCTATTGGGATTTTGCTTGGAAGGGGAAGAAAAGATGCTTGTTTATGAATACGTCCCCAACAAAAGCCTCGACTATATTCTATTTGACCCTGAGAAACAAGGACAACTGGATTGGTCGAGACGTTACAAAATTATTGCGGGGATTGCCCGAGGAATTATGTATCTACATGAAGACTCCCGGCTAAGAATTATACATCGTGATCTTAAAGCCAGCAATATATTGTTAGATGGGGAAATGCATCCAAAAATATCTGATTTTGGCATGGCGAGGATATTCGGCGTTGATCAAACTCAAGCAAACACAAATAGAATTGTTGGAACATATGGTTACATGTCTCCAGAATATGCAATGCATGGACAATTTTCTATCAAGTCCGATATCTATAGTTTTGGTGTCTTAGTTCTAGAAATTATCAGTGGCAAGAAGAATAGTTTCCTCTTCCAAACAGATGCAGCTGAGCACCTCGTGAGCCATGCTTGGAAACTATGGAGAGATGGAACTCCTTTGGAATTTTTGGATCCGTGTCTGAGAGATTCTTATTCTAGAAATGAAGTTATCAGATGTATCCACATTGGCTTACTTTGTGTTCAGGAAGATCCGGCTGACAGACCTACAATGCAATCACTAGTTCTTATGCTCAACAGCTACTCTGTTACTCTACCATTACCTCAAGAGCCAGCATTTGTCCATAGGACTAAATCGGAGTTCATGCCAAAGATACCGCTGGAGTATGATCAGTCTATCAGCAAGTCAAGTCCATCGGTTTATGAAGGATCAATCACTGAATTGACCGCCGAGTCTGAGACGCTTGCAACCTCACAACTCCTGCCAATTTATAGCAACATCTCAAACAGGGCAGCTGTACCTTCCTTATGCAAATCGTTAGCGTTGAAGCTCAAGCACCAACTTACCGGTATCTTTTCTGTTCAAACACAACCAATTTCACTCCCAACAGCTCCTACCAGTCTAATCGAAATCTCCTCCTCTCTTCTCTCACATCCAACGCCACACGTGATATTGGATTCTACTATACCACAGCGGGCGTGCAAAACTCAACTGCAGAAGTACGTAGTTTACGGTTCTTTCCTATGCCGTGGAGATCTCACCCCTGAGTCGTGCCAAGAATGTGTTTCTACAATAGCCAAAGATGGTGTCCAAGAGCACTGCCCCTTGGGAAAAATCGCAATAATATGGTACGACGAGTGCATGTTGCGCTACGCAAACAAGTCTTACTTGAACAGAATGGACGAAGCTCCTCGAGTTTCCATGTGGAACACTGGGAATATCACTGACCCTACCCGCTTTTTGCAGCTGGCGGCAGAAACAATCAATGGCTTGGTGGCTCCGGCTTCAAATGCACCCTCCGGTGCTAAAAAGTATGCAACAAAAGAAGCAGACTTCAACGGGTTTCAGCAGTTTTACAGCCTTGTGCAGTGCACACCAGACCTACCAAGCACAAGTTGTGTTAAATGTCTTCTAGAAGCTATTGCGCAAATTCCAGATTGTTGTACTGGAAAGCTAGGTGGTAGAGTTTTATATCCTAGTTGTAATTTTCGATATGAACTTTATCCATTTTATGCAACTGAAGCAGCCACACCTTCACCACCGCCACAACCTTTGACTCTTCCTCCCCCACCTCCAGCAGGAAACGACCAAATCTCATCCTTAATCATAGCGGCCATTGTTGTTCCAATTGCCATAGTGCTTTTCGATCTTGGGGAGAATGAAATTACTACTGAGAAGTCATTGCAGTTTGATTTTGCTACAATTGAAGCAGCCACAAACCAGTTCTCTGTTAATAATAGATTAGGGGAAGGTGGCTTTGGAGAGGTTTACAAGGGAACACTTCCTAATGGGCAAGAAATAGCTGTGAAGAGGTTATCTAGAAGCTCTGGGCAAGGTGTCATGGAATTTAAGAATGAGGTGGTTTTGGTAGCCAAGCTTCAGCACAGAAATTTAGTGAGGCTACTGGGATTTTGCTCTGAAGGAGAAGAAAAGATTCTTATCTATGAACTTGTGCAGAACAAAAGCCTTGACCACTTTTTATTCGACTCGGATATTCAAGTAAAGTTGGATTGGTCAAGCAGATACAATATTATAGGGGGGATTGCTAGAGGGATTCTCTATCTCCATCAAGATTCTCGGCTTAAAATCATACATCGCGATCTCAAAGCAAGCAACATATTGTTAGATGGAGAAATGAACCCCAAAGTATCAGATTTTGGAATGGCAAGAATATTTCGAGTTGATCAAACTCAAGGAAGCACCAGAAGGATTGTTGGAACTTATGGTTACATGCCTCCAGAGTATGCAATGCATGGACAGTTCTCGGAGAAGTCTGATGTGTACAGTCTTGGTGTCTTAATCTTAGAGATTGTAACAGGCAAGAAAAACACTAGTTTCTATCAATCAGACCGTGGCGAAGATCTCTTAACATATGAAGATCCAGAGGAGAGACCTACTATGCAAACCATAGTTCTCATGCTAAGCAGTTACTCAGTTACTATGTCATTGCCTCAAAAGCCAGCATTTTTCATGCATGGTAAAGCAGAATTGAACATGCCATCTTTATCCCCTGGATCATCATATCAACCCACTAGCAGCGCAGTCTCATATTCTGTTAATGAAGCTTCAATTACTGAATTATTCCCTCGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0042742 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050896 GO:0051704 GO:0051707 GO:0071704 GO:0071944 GO:0098542 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr Stress-antifung

Protein Analysis

1276

Amino Acids

141.03

Weight (kDa)

6.04

Isoelectric Point (pI)

47.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Stress-antifung	PF01657	29 - 125	2.2e-27	Salt stress response/antifungal
Stress-antifung	PF01657	147 - 236	2.8e-11	Salt stress response/antifungal
Pkinase	PF00069	334 - 601	1.6e-45	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	336 - 602	4.8e-48	Protein tyrosine and serine/threonine kinase
Stress-antifung	PF01657	746 - 799	1.3e-13	Salt stress response/antifungal
Stress-antifung	PF01657	819 - 913	1.4e-09	Salt stress response/antifungal
Pkinase	PF00069	989 - 1190	4.6e-43	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	990 - 1194	1.2e-44	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0000283)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G45860 AT4G05200 AT4G05200 AT4G11521 AT4G11530 AT4G23130 AT4G23130 AT4G23130 AT4G23140 AT4G23140 AT4G23150 AT4G23160 AT4G23170 AT4G23180 AT4G23180 AT4G23180 AT4G23230 AT4G23270 AT4G23270 AT4G23270 AT4G23280 AT4G23280 AT4G23280 AT4G23310 AT4G23310
fragaria_vesca	FvH4_3g02772 FvH4_3g02772 FvH4_3g02773 FvH4_3g02773 FvH4_3g02774 FvH4_3g02800 FvH4_3g02820 FvH4_3g02820 FvH4_3g02830
malus_domestica	MD00G1101900.v1.1 MD00G1102100.v1.1 MD00G1102200.v1.1 MD01G1098200.v1.1 MD05G1340000.v1.1 MD05G1340300.v1.1 MD07G1014100.v1.1 MD07G1014300.v1.1 MD07G1014400.v1.1 MD07G1014700.v1.1 MD10G1312900.v1.1 MD11G1022600.v1.1 MD11G1291500.v1.1 MD14G1207100.v1.1 MD16G1096600.v1.1
prunus_persica	Prupe.4G027300_v2.0.a1 Prupe.4G027400_v2.0.a1 Prupe.4G027700_v2.0.a1 Prupe.4G027800_v2.0.a1
pyrus_communis	pycom05g30960 pycom10g00940 pycom10g00950 pycom10g26430 pycom10g26450 pycom10g26460 pycom10g26470 pycom10g26480 pycom10g26490 pycom10g26500 pycom10g26510 pycom10g26570 pycom11g01820 pycom11g04000 pycom14g17130 pycom14g17140 pycom15g28820
rosa_chinensis	RchiOBHm_Chr4g0408141 RchiOBHm_Chr5g0004211 RchiOBHm_Chr5g0004231 RchiOBHm_Chr5g0004241 RchiOBHm_Chr5g0004261
rosa_laevigata	RLG00000008668 RLG00000008672 RLG00000031212 RLG00000031217
rosa_multiflora	Rmu_co8162658.1_g000001 Rmu_co8336297.1_g000001 Rmu_sc0002073.1_g000001 Rmu_sc0004964.1_g000002 Rmu_sc0004964.1_g000003 Rmu_sc0004964.1_g000007 Rmu_sc0006006.1_g000007 Rmu_sc0007868.1_g000015 Rmu_sc0007868.1_g000016 Rmu_sc0007868.1_g000017 Rmu_sc0007868.1_g000021 Rmu_sc0007868.1_g000023 Rmu_sc0019654.1_g000002 Rmu_sc0038540.1_g000001
rosa_roxburghii	Rroxscaffold_1G00071450 Rroxscaffold_1G00071460 Rroxscaffold_1G00071470 Rroxscaffold_1G00071560 Rroxscaffold_1G00071720 Rroxscaffold_1G00071770 Rroxscaffold_5G00352640
rosa_rugosa	Rorug04G0083700 Rorug04G0410700 Rorug04G0410800 Rorug04G0410900
rosa_samantha	Rh4BG145400 Rh4CG154900 Rh4DG141900 Rh5BG037500 Rh5BG037600 Rh5BG037700 Rh5CG041000 Rh5CG041100 Rh5CG041200 Rh5CG041300 Rh5CG041400 Rh5DG037100
rosa_wichuraiana	Rw4G012130 Rw5G003450 Rw5G003460 Rw5G003470 Rw5G003480 Rw5G003640 Rw5G003660 Rw5G003670 Rw5G003680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	720
AasI	GACNNNNNNGTC	1 cut(s)	1969
AatII	GACGTC	2 cut(s)	255, 731
Acc16I	TGCGCA	1 cut(s)	2664
Acc65I	GGTACC	1 cut(s)	971
AccB1I	GGYRCC	3 cut(s)	739, 745, 971
AccB7I	CCANNNNNTGG	1 cut(s)	466
AccI	GTMKAC	2 cut(s)	593, 1041
AccII	CGCG	4 cut(s)	174, 246, 524, 3336
AclWI	GGATC	7 cut(s)	7, 1685, 1698, 1763, 1954, 3611, 3760
AcoI	YGGCCR	4 cut(s)	202, 489, 822, 2850
AcsI	RAATTY	6 cut(s)	634, 1118, 1682, 2667, 3083, 3127
AcuI	CTGAAG	3 cut(s)	2778, 3101, 3177
AcvI	CACGTG	1 cut(s)	2185
AcyI	GRCGYC	5 cut(s)	219, 252, 728, 740, 746
AdeI	CACNNNGTG	1 cut(s)	1639
AfiI	CCNNNNNNNGG	5 cut(s)	31, 466, 749, 1165, 2816
AflIII	ACRYGT	2 cut(s)	1503, 2182
AgeI	ACCGGT	2 cut(s)	450, 2084
AhlI	ACTAGT	2 cut(s)	1798, 3569
AjnI	CCWGG	4 cut(s)	459, 748, 806, 3748
AjuI	GAANNNNNNNTTGG	6 cut(s)	362, 394, 1014, 1046, 2979, 3011
Alw21I	GWGCWC	6 cut(s)	581, 779, 1114, 1635, 2337, 2612
Alw26I	GTCTC	7 cut(s)	212, 317, 1285, 1512, 1970, 3622, 3787
Alw44I	GTGCAC	3 cut(s)	577, 1110, 2608
AlwI	GGATC	7 cut(s)	7, 1685, 1698, 1763, 1954, 3611, 3760
AlwNI	CAGNNNCTG	1 cut(s)	276
Ama87I	CYCGRG	2 cut(s)	1320, 2430
AoxI	GGCC	6 cut(s)	202, 229, 489, 822, 1102, 2850
ApaLI	GTGCAC	3 cut(s)	577, 1110, 2608
ApoI	RAATTY	6 cut(s)	634, 1118, 1682, 2667, 3083, 3127
ArsI	GACNNNNNNTTYG	2 cut(s)	2310, 2342
AsiGI	ACCGGT	2 cut(s)	450, 2084
Asp700I	GAANNNNTTC	3 cut(s)	452, 1604, 3171
Asp718I	GGTACC	1 cut(s)	971
AspLEI	GCGC	6 cut(s)	312, 742, 748, 2388, 2665, 3779
AspS9I	GGNCC	1 cut(s)	375
AsuC2I	CCSGG	2 cut(s)	317, 1351
AsuHPI	GGTGA	6 cut(s)	28, 97, 247, 260, 2269, 2766
AsuNHI	GCTAGC	1 cut(s)	473
AvaI	CYCGRG	2 cut(s)	1320, 2430
AvaII	GGWCC	1 cut(s)	375
BaeGI	GKGCMC	3 cut(s)	581, 1114, 2612
BalI	TGGCCA	2 cut(s)	491, 824
BamHI	GGATCC	1 cut(s)	1690
BanI	GGYRCC	3 cut(s)	739, 745, 971
BanII	GRGCYC	1 cut(s)	757
BarI	GAAGNNNNNNTAC	4 cut(s)	1134, 1166, 3099, 3131
BauI	CACGAG	1 cut(s)	1637
BbrPI	CACGTG	1 cut(s)	2185
BbsI	GAAGAC	3 cut(s)	94, 1350, 2639
Bbv12I	GWGCWC	6 cut(s)	581, 779, 1114, 1635, 2337, 2612
BccI	CCATC	9 cut(s)	1055, 1126, 1394, 1661, 1939, 2315, 3312, 3359, 3745
BceAI	ACGGC	6 cut(s)	83, 189, 244, 526, 815, 2250
BciT130I	CCWGG	4 cut(s)	461, 750, 808, 3750
BciVI	GTATCC	2 cut(s)	690, 1748
BclI	TGATCA	3 cut(s)	1456, 1906, 3421
BcnI	CCSGG	2 cut(s)	317, 1351
BcoDI	GTCTC	7 cut(s)	212, 317, 1285, 1512, 1970, 3622, 3787
BcuI	ACTAGT	2 cut(s)	1798, 3569
BfmI	CTRYAG	2 cut(s)	1555, 2231
BfoI	RGCGCY	3 cut(s)	313, 743, 749
BfuI	GTATCC	2 cut(s)	690, 1748
BglI	GCCNNNNNGGC	1 cut(s)	497
BglII	AGATCT	2 cut(s)	2272, 3598
BlpI	GCTNAGC	3 cut(s)	44, 1629, 3659
Bme1390I	CCNGG	6 cut(s)	317, 461, 750, 808, 1351, 3750
Bme18I	GGWCC	1 cut(s)	375
BmeT110I	CYCGRG	2 cut(s)	1320, 2430
BmgT120I	GGNCC	1 cut(s)	375
BmiI	GGNNCC	7 cut(s)	422, 625, 741, 747, 973, 1692, 2517
BmrFI	CCNGG	6 cut(s)	317, 461, 750, 808, 1351, 3750
BmrI	ACTGGG	2 cut(s)	2460, 3152
BmsI	GCATC	3 cut(s)	1197, 1420, 1612
BmtI	GCTAGC	1 cut(s)	477
BmuI	ACTGGG	2 cut(s)	2460, 3152
BpiI	GAAGAC	3 cut(s)	94, 1350, 2639
BplI	GAGNNNNNCTC	2 cut(s)	3279, 3311
BpmI	CTGGAG	4 cut(s)	1494, 1919, 2796, 3459
Bpu1102I	GCTNAGC	3 cut(s)	44, 1629, 3659
BpuEI	CTTGAG	4 cut(s)	1449, 1827, 2054, 3414
BpuMI	CCSGG	2 cut(s)	317, 1351
BsaAI	YACGTR	2 cut(s)	2185, 2242
BsaBI	GATNNNNATC	1 cut(s)	3176
BsaHI	GRCGYC	5 cut(s)	219, 252, 728, 740, 746
BsaI	GGTCTC	1 cut(s)	3622
BsaWI	WCCGGW	3 cut(s)	450, 2084, 2537
BsaXI	ACNNNNNCTCC	6 cut(s)	131, 161, 182, 212, 2785, 2815
Bsc4I	CCNNNNNNNGG	5 cut(s)	31, 466, 749, 1165, 2816
Bse118I	RCCGGY	5 cut(s)	179, 450, 736, 742, 2084
Bse1I	ACTGG	8 cut(s)	124, 1143, 1284, 2136, 2455, 2689, 2959, 3147
Bse3DI	GCAATG	6 cut(s)	627, 825, 1527, 2918, 3492, 3683
Bse8I	GATNNNNATC	1 cut(s)	3176
BseBI	CCWGG	4 cut(s)	461, 750, 808, 3750
BseGI	GGATG	3 cut(s)	1411, 2170, 2834
BseJI	GATNNNNATC	1 cut(s)	3176
BseLI	CCNNNNNNNGG	5 cut(s)	31, 466, 749, 1165, 2816
BseMI	GCAATG	6 cut(s)	627, 825, 1527, 2918, 3492, 3683
BseMII	CTCAG	8 cut(s)	1113, 1254, 1620, 1691, 1965, 2274, 2901, 3684
BseNI	ACTGG	8 cut(s)	124, 1143, 1284, 2136, 2455, 2689, 2959, 3147
BseRI	GAGGAG	6 cut(s)	134, 148, 2143, 2146, 2418, 3638
BseSI	GKGCMC	3 cut(s)	581, 1114, 2612
BseX3I	CGGCCG	1 cut(s)	202
BsgI	GTGCAG	2 cut(s)	2624, 3212
Bsh1236I	CGCG	4 cut(s)	174, 246, 524, 3336
Bsh1285I	CGRYCG	2 cut(s)	205, 321
BshFI	GGCC	6 cut(s)	204, 231, 491, 824, 1104, 2852
BshNI	GGYRCC	3 cut(s)	739, 745, 971
BshTI	ACCGGT	2 cut(s)	450, 2084
BsiEI	CGRYCG	2 cut(s)	205, 321
BsiHKAI	GWGCWC	6 cut(s)	581, 779, 1114, 1635, 2337, 2612
BsiHKCI	CYCGRG	2 cut(s)	1320, 2430
BslFI	GGGAC	3 cut(s)	374, 681, 1211
BslI	CCNNNNNNNGG	5 cut(s)	31, 466, 749, 1165, 2816
BsmAI	GTCTC	7 cut(s)	212, 317, 1285, 1512, 1970, 3622, 3787
BsmBI	CGTCTC	3 cut(s)	212, 1285, 1970
BsmFI	GGGAC	3 cut(s)	374, 681, 1211
BsnI	GGCC	6 cut(s)	204, 231, 491, 824, 1104, 2852
Bso31I	GGTCTC	1 cut(s)	3622
BsoBI	CYCGRG	2 cut(s)	1320, 2430
Bsp1286I	GDGCHC	7 cut(s)	581, 757, 779, 1114, 1635, 2337, 2612
Bsp1407I	TGTACA	2 cut(s)	561, 3519
Bsp1720I	GCTNAGC	3 cut(s)	44, 1629, 3659
Bsp68I	TCGCGA	2 cut(s)	524, 3336
BspANI	GGCC	6 cut(s)	204, 231, 491, 824, 1104, 2852
BspCNI	CTCAG	8 cut(s)	1112, 1255, 1621, 1692, 1966, 2275, 2902, 3683
BspFNI	CGCG	4 cut(s)	174, 246, 524, 3336
BspLI	GGNNCC	7 cut(s)	422, 625, 741, 747, 973, 1692, 2517
BspMAI	CTGCAG	1 cut(s)	2235
BspOI	GCTAGC	1 cut(s)	477
BspPI	GGATC	7 cut(s)	7, 1685, 1698, 1763, 1954, 3611, 3760
BspQI	GCTCTTC	1 cut(s)	788
BspT107I	GGYRCC	3 cut(s)	739, 745, 971
BspTNI	GGTCTC	1 cut(s)	3622
BsrDI	GCAATG	6 cut(s)	627, 825, 1527, 2918, 3492, 3683
BsrFI	RCCGGY	5 cut(s)	179, 450, 736, 742, 2084
BsrGI	TGTACA	2 cut(s)	561, 3519
BsrI	ACTGG	8 cut(s)	124, 1143, 1284, 2136, 2455, 2689, 2959, 3147
BssAI	RCCGGY	5 cut(s)	179, 450, 736, 742, 2084
BssNI	GRCGYC	5 cut(s)	219, 252, 728, 740, 746
BssSI	CACGAG	1 cut(s)	1637
BssT1I	CCWWGG	2 cut(s)	1105, 2343
Bst2BI	CACGAG	1 cut(s)	1637
Bst2UI	CCWGG	4 cut(s)	461, 750, 808, 3750
Bst4CI	ACNGT	6 cut(s)	612, 781, 2252, 3498, 3524, 3590
Bst6I	CTCTTC	6 cut(s)	239, 788, 1616, 2166, 2802, 3041
BstACI	GRCGYC	5 cut(s)	219, 252, 728, 740, 746
BstAPI	GCANNNNNTGC	1 cut(s)	276
BstAUI	TGTACA	2 cut(s)	561, 3519
BstBAI	YACGTR	2 cut(s)	2185, 2242
BstDSI	CCRYGG	4 cut(s)	244, 492, 2266, 3589
BstEII	GGTNACC	1 cut(s)	34
BstENI	CCTNNNNNAGG	1 cut(s)	2814
BstF5I	GGATG	3 cut(s)	1411, 2170, 2834
BstFNI	CGCG	4 cut(s)	174, 246, 524, 3336
BstH2I	RGCGCY	3 cut(s)	313, 743, 749
BstHHI	GCGC	6 cut(s)	312, 742, 748, 2388, 2665, 3779
BstMAI	GTCTC	7 cut(s)	212, 317, 1285, 1512, 1970, 3622, 3787
BstMCI	CGRYCG	2 cut(s)	205, 321
BstNI	CCWGG	4 cut(s)	461, 750, 808, 3750
BstNSI	RCATGY	5 cut(s)	358, 1507, 2383, 3472, 3736
BstPI	GGTNACC	1 cut(s)	34
BstSCI	CCNGG	6 cut(s)	315, 459, 748, 806, 1349, 3748
BstSFI	CTRYAG	2 cut(s)	1555, 2231
BstSLI	GKGCMC	3 cut(s)	581, 1114, 2612
BstSNI	TACGTA	1 cut(s)	2242
BstUI	CGCG	4 cut(s)	174, 246, 524, 3336
BstV2I	GAAGAC	3 cut(s)	94, 1350, 2639
BstX2I	RGATCY	5 cut(s)	1690, 1768, 2272, 3598, 3616
BstYI	RGATCY	5 cut(s)	1690, 1768, 2272, 3598, 3616
BstZI	CGGCCG	1 cut(s)	202
BsuI	GTATCC	2 cut(s)	690, 1748
BsuRI	GGCC	6 cut(s)	204, 231, 491, 824, 1104, 2852
BtgI	CCRYGG	4 cut(s)	244, 492, 2266, 3589
BtgZI	GCGATG	1 cut(s)	3317
BtsCI	GGATG	3 cut(s)	1411, 2170, 2834
BtsI	GCAGTG	3 cut(s)	581, 2335, 2612
BtsIMutI	CAGTG	9 cut(s)	36, 581, 786, 1594, 1953, 2335, 2448, 2460, 2612
BtuMI	TCGCGA	2 cut(s)	524, 3336
CaiI	CAGNNNCTG	1 cut(s)	276
CfoI	GCGC	6 cut(s)	312, 742, 748, 2388, 2665, 3779
Cfr10I	RCCGGY	5 cut(s)	179, 450, 736, 742, 2084
Cfr13I	GGNCC	1 cut(s)	375
Cfr42I	CCGCGG	1 cut(s)	247
CseI	GACGC	2 cut(s)	227, 1987
CsiI	ACCWGGT	1 cut(s)	459
CspAI	ACCGGT	2 cut(s)	450, 2084
CspCI	CAANNNNNGTGG	2 cut(s)	2772, 2807
DinI	GGCGCC	2 cut(s)	741, 747
DraIII	CACNNNGTG	1 cut(s)	1639
DrdI	GACNNNNNNGTC	1 cut(s)	1969
DseDI	GACNNNNNNGTC	1 cut(s)	1969
EaeI	YGGCCR	4 cut(s)	202, 489, 822, 2850
EagI	CGGCCG	1 cut(s)	202
Eam1104I	CTCTTC	6 cut(s)	239, 788, 1616, 2166, 2802, 3041
EarI	CTCTTC	6 cut(s)	239, 788, 1616, 2166, 2802, 3041
EciI	GGCGGA	1 cut(s)	759
EclXI	CGGCCG	1 cut(s)	202
Eco105I	TACGTA	1 cut(s)	2242
Eco130I	CCWWGG	2 cut(s)	1105, 2343
Eco24I	GRGCYC	1 cut(s)	757
Eco31I	GGTCTC	1 cut(s)	3622
Eco32I	GATATC	1 cut(s)	1552
Eco47I	GGWCC	1 cut(s)	375
Eco52I	CGGCCG	1 cut(s)	202
Eco57I	CTGAAG	3 cut(s)	2778, 3101, 3177
Eco72I	CACGTG	1 cut(s)	2185
Eco88I	CYCGRG	2 cut(s)	1320, 2430
Eco91I	GGTNACC	1 cut(s)	34
EcoNI	CCTNNNNNAGG	1 cut(s)	2814
EcoO109I	RGGNCCY	1 cut(s)	375
EcoO65I	GGTNACC	1 cut(s)	34
EcoRII	CCWGG	4 cut(s)	459, 748, 806, 3748
EcoRV	GATATC	1 cut(s)	1552
EcoT14I	CCWWGG	2 cut(s)	1105, 2343
EcoT22I	ATGCAT	4 cut(s)	1413, 1527, 3492, 3714
EcoT38I	GRGCYC	1 cut(s)	757
EgeI	GGCGCC	2 cut(s)	741, 747
EheI	GGCGCC	2 cut(s)	741, 747
ErhI	CCWWGG	2 cut(s)	1105, 2343
Esp3I	CGTCTC	3 cut(s)	212, 1285, 1970
FalI	AAGNNNNNCTT	3 cut(s)	32, 2390, 2422
FaqI	GGGAC	3 cut(s)	374, 681, 1211
FauI	CCCGC	5 cut(s)	400, 647, 1303, 2205, 2480
FauNDI	CATATG	2 cut(s)	1495, 3610
FbaI	TGATCA	3 cut(s)	1456, 1906, 3421
FblI	GTMKAC	2 cut(s)	593, 1041
FokI	GGATG	3 cut(s)	1398, 2157, 2821
FriOI	GRGCYC	1 cut(s)	757
FspI	TGCGCA	1 cut(s)	2664
GlaI	GCGC	6 cut(s)	311, 741, 747, 2387, 2664, 3778
GsuI	CTGGAG	4 cut(s)	1494, 1919, 2796, 3459
HaeII	RGCGCY	3 cut(s)	313, 743, 749
HaeIII	GGCC	6 cut(s)	204, 231, 491, 824, 1104, 2852
HgaI	GACGC	2 cut(s)	227, 1987
HhaI	GCGC	6 cut(s)	312, 742, 748, 2388, 2665, 3779
Hin1I	GRCGYC	5 cut(s)	219, 252, 728, 740, 746
Hin6I	GCGC	6 cut(s)	310, 740, 746, 2386, 2663, 3777
HinP1I	GCGC	6 cut(s)	310, 740, 746, 2386, 2663, 3777
HindIII	AAGCTT	4 cut(s)	46, 1148, 3113, 3801
HphI	GGTGA	6 cut(s)	28, 97, 247, 260, 2269, 2766
Hpy166II	GTNNAC	8 cut(s)	579, 594, 1042, 1112, 2248, 2610, 3007, 3520
Hpy8I	GTNNAC	8 cut(s)	579, 594, 1042, 1112, 2248, 2610, 3007, 3520
Hpy99I	CGWCG	5 cut(s)	221, 512, 529, 730, 2375
HpyCH4III	ACNGT	6 cut(s)	612, 781, 2252, 3498, 3524, 3590
HpyCH4IV	ACGT	7 cut(s)	252, 432, 728, 1224, 1294, 2184, 2241
HpySE526I	ACGT	7 cut(s)	252, 432, 728, 1224, 1294, 2184, 2241
Hsp92I	GRCGYC	5 cut(s)	219, 252, 728, 740, 746
HspAI	GCGC	6 cut(s)	310, 740, 746, 2386, 2663, 3777
KasI	GGCGCC	2 cut(s)	739, 745
KpnI	GGTACC	1 cut(s)	975
KroI	GCCGGC	1 cut(s)	742
KroNI	GCCGGC	1 cut(s)	744
Ksp22I	TGATCA	3 cut(s)	1456, 1906, 3421
KspI	CCGCGG	1 cut(s)	247
LguI	GCTCTTC	1 cut(s)	788
LmnI	GCTCC	4 cut(s)	623, 2134, 2431, 2521
LweI	GCATC	3 cut(s)	1197, 1420, 1612
MabI	ACCWGGT	1 cut(s)	459
MaeII	ACGT	7 cut(s)	252, 432, 728, 1224, 1294, 2184, 2241
MfeI	CAATTG	3 cut(s)	837, 2865, 2940
MflI	RGATCY	5 cut(s)	1690, 1768, 2272, 3598, 3616
MhlI	GDGCHC	7 cut(s)	581, 757, 779, 1114, 1635, 2337, 2612
MlsI	TGGCCA	2 cut(s)	491, 824
MluNI	TGGCCA	2 cut(s)	491, 824
Mly113I	GGCGCC	2 cut(s)	740, 746
MlyI	GAGTC	7 cut(s)	407, 959, 1340, 1979, 2294, 2788, 3218
MmeI	TCCRAC	8 cut(s)	77, 153, 186, 393, 1468, 2198, 3225, 3433
Mox20I	TGGCCA	2 cut(s)	491, 824
Mph1103I	ATGCAT	4 cut(s)	1413, 1527, 3492, 3714
MreI	CGCCGGCG	1 cut(s)	742
MroNI	GCCGGC	1 cut(s)	742
MroXI	GAANNNNTTC	3 cut(s)	452, 1604, 3171
MscI	TGGCCA	2 cut(s)	491, 824
Msp20I	TGGCCA	2 cut(s)	491, 824
MspA1I	CMGCKG	7 cut(s)	246, 263, 1628, 1897, 2033, 2212, 2485
MspR9I	CCNGG	6 cut(s)	317, 461, 750, 808, 1351, 3750
MunI	CAATTG	3 cut(s)	837, 2865, 2940
MvaI	CCWGG	4 cut(s)	461, 750, 808, 3750
MvnI	CGCG	4 cut(s)	174, 246, 524, 3336
NaeI	GCCGGC	1 cut(s)	744
NarI	GGCGCC	2 cut(s)	740, 746
NciI	CCSGG	2 cut(s)	317, 1351
NdeI	CATATG	2 cut(s)	1495, 3610
NgoMIV	GCCGGC	1 cut(s)	742
NheI	GCTAGC	1 cut(s)	473
NlaIV	GGNNCC	7 cut(s)	422, 625, 741, 747, 973, 1692, 2517
NmeAIII	GCCGAG	5 cut(s)	421, 666, 798, 1993, 3295
NmuCI	GTSAC	5 cut(s)	34, 248, 253, 280, 781
NruI	TCGCGA	2 cut(s)	524, 3336
NsbI	TGCGCA	1 cut(s)	2664
NsiI	ATGCAT	4 cut(s)	1413, 1527, 3492, 3714
NspI	RCATGY	5 cut(s)	358, 1507, 2383, 3472, 3736
PaeI	GCATGC	1 cut(s)	358
PaeR7I	CTCGAG	1 cut(s)	2430
PciI	ACATGT	1 cut(s)	1503
PciSI	GCTCTTC	1 cut(s)	788
PcsI	WCGNNNNNNNCGW	3 cut(s)	171, 438, 533
PdiI	GCCGGC	1 cut(s)	744
PdmI	GAANNNNTTC	3 cut(s)	452, 1604, 3171
PfeI	GAWTC	5 cut(s)	1706, 2195, 3172, 3292, 3311
PflMI	CCANNNNNTGG	1 cut(s)	466
PfoI	TCCNGGA	1 cut(s)	806
PinAI	ACCGGT	2 cut(s)	450, 2084
PleI	GAGTC	7 cut(s)	406, 958, 1340, 1978, 2293, 2788, 3218
PluTI	GGCGCC	2 cut(s)	743, 749
PmaCI	CACGTG	1 cut(s)	2185
PmlI	CACGTG	1 cut(s)	2185
PpsI	GAGTC	7 cut(s)	406, 958, 1340, 1978, 2293, 2788, 3218
Ppu21I	YACGTR	2 cut(s)	2185, 2242
PpuMI	RGGWCCY	1 cut(s)	375
PscI	ACATGT	1 cut(s)	1503
PsiI	TTATAA	1 cut(s)	720
Psp5II	RGGWCCY	1 cut(s)	375
Psp6I	CCWGG	4 cut(s)	459, 748, 806, 3748
PspCI	CACGTG	1 cut(s)	2185
PspEI	GGTNACC	1 cut(s)	34
PspGI	CCWGG	4 cut(s)	459, 748, 806, 3748
PspN4I	GGNNCC	7 cut(s)	422, 625, 741, 747, 973, 1692, 2517
PspPI	GGNCC	1 cut(s)	375
PspPPI	RGGWCCY	1 cut(s)	375
PspXI	VCTCGAGB	1 cut(s)	2430
PstI	CTGCAG	1 cut(s)	2235
PstNI	CAGNNNCTG	1 cut(s)	276
PsuI	RGATCY	5 cut(s)	1690, 1768, 2272, 3598, 3616
PvuII	CAGCTG	3 cut(s)	1628, 2033, 2485
RruI	TCGCGA	2 cut(s)	524, 3336
SacII	CCGCGG	1 cut(s)	247
SapI	GCTCTTC	1 cut(s)	788
Sau96I	GGNCC	1 cut(s)	375
SchI	GAGTC	7 cut(s)	407, 959, 1340, 1979, 2294, 2788, 3218
ScrFI	CCNGG	6 cut(s)	317, 461, 750, 808, 1351, 3750
SduI	GDGCHC	7 cut(s)	581, 757, 779, 1114, 1635, 2337, 2612
SexAI	ACCWGGT	1 cut(s)	459
SfaNI	GCATC	3 cut(s)	1197, 1420, 1612
SfcI	CTRYAG	2 cut(s)	1555, 2231
SfoI	GGCGCC	2 cut(s)	741, 747
Sfr274I	CTCGAG	1 cut(s)	2430
Sfr303I	CCGCGG	1 cut(s)	247
SgrAI	CRCCGGYG	2 cut(s)	736, 742
SgrBI	CCGCGG	1 cut(s)	247
SinI	GGWCC	1 cut(s)	375
SlaI	CTCGAG	1 cut(s)	2430
SmlI	CTYRAG	5 cut(s)	1464, 1842, 2069, 2430, 3429
SmoI	CTYRAG	5 cut(s)	1464, 1842, 2069, 2430, 3429
SnaBI	TACGTA	1 cut(s)	2242
SpeI	ACTAGT	2 cut(s)	1798, 3569
SphI	GCATGC	1 cut(s)	358
SspDI	GGCGCC	2 cut(s)	739, 745
SspI	AATATT	2 cut(s)	3268, 3411
StyD4I	CCNGG	6 cut(s)	315, 459, 748, 806, 1349, 3748
StyI	CCWWGG	2 cut(s)	1105, 2343
TaaI	ACNGT	6 cut(s)	612, 781, 2252, 3498, 3524, 3590
TaiI	ACGT	7 cut(s)	255, 435, 731, 1227, 1297, 2187, 2244
TatI	WGTACW	4 cut(s)	561, 903, 2681, 3519
TauI	GCSGC	7 cut(s)	224, 246, 310, 473, 654, 2492, 2852
TfiI	GAWTC	5 cut(s)	1706, 2195, 3172, 3292, 3311
TscAI	CASTG	9 cut(s)	36, 581, 786, 1594, 1960, 2342, 2455, 2467, 2612
TseFI	GTSAC	5 cut(s)	34, 248, 253, 280, 781
Tsp45I	GTSAC	5 cut(s)	34, 248, 253, 280, 781
TspGWI	ACGGA	4 cut(s)	74, 497, 509, 1683
TspRI	CASTG	9 cut(s)	36, 581, 786, 1594, 1960, 2342, 2455, 2467, 2612
Van91I	CCANNNNNTGG	1 cut(s)	466
VneI	GTGCAC	3 cut(s)	577, 1110, 2608
VpaK11BI	GGWCC	1 cut(s)	375
XagI	CCTNNNNNAGG	1 cut(s)	2814
XapI	RAATTY	6 cut(s)	634, 1118, 1682, 2667, 3083, 3127
XbaI	TCTAGA	4 cut(s)	1576, 1715, 2650, 3056
XceI	RCATGY	5 cut(s)	358, 1507, 2383, 3472, 3736
XhoI	CTCGAG	1 cut(s)	2430
XmiI	GTMKAC	2 cut(s)	593, 1041
XmnI	GAANNNNTTC	3 cut(s)	452, 1604, 3171
ZraI	GACGTC	2 cut(s)	253, 729
Zsp2I	ATGCAT	4 cut(s)	1413, 1527, 3492, 3714

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.