Rw4G012130

Cysteine-rich receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000283
Physical Location & Seq
Reverse (-)
28526970 .. 28528231
1262 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G012130.1

Sequence Viewer

Length: 1032 bp
ATGGCCCTTGTGGTGCTCCCCATTCTTGGCTTCCTTAGAGTTAGGGTTGAAGCTCAAGCACCAACTTACCGGTATCATATCTGTTCAAACACAACCAGTTTCACTCCCAACAGCACCTACCAGTCTAATCTTAATCTTCTCCTCTCTTCTCTCACATCCAACGCCACACGTGATATCGGATTCTACTACACCAAAGCGGGCGTGCAAAACTCAACTTCAGAAGTAGTTTACGGTTCTTTCCTATGCCGTGGAGATCTCACCCCTGACTTGTGCCAAGAATGTATTACTACTATAGCCAAAGATGGTGTCCAAAAGTACTGCCCCTTGGAAAAAATTTCAATAATATGGTACGATGGGTGCATGTTGCGCTACACAAATCAGTCTTTCTTGAACAGGTTGGACAAAGATCCTCAAATTTCCATGCTGCTAGCAGAAACAATCAATGGCTTGGTGGCTCTGGCTTCAAATGCACCATCTGGTGCTAAAAAGTATGCAACGAAAGAAGCACCTTTCACAGGGTTTCAGCAGTTGAACAGCCTTGTTCAGTGCACACCAGACATATCAAGCACAAGTTGTGATACATGTCTTCGAGAAGCTATTGCGCAACTTTCAGGTTGTTGTACTGGAAAGGAAAGTGCTAGAATTTTATCTCCTAGTTGTAATGTTCGATACGAAGTTTATCCATTTTATACAACTGAAGCAGTCACACCTTCACCCCTCCCACAACCTCCGACTCTTCCTCCCCCACCTCCAGGAAAAGACCAAATCTCATCCTTAATCATAGTGGCCATTGTTGTTCCAATTTCCATAGTGCTTTTATTGGTAGGCTGCTTTTTTCTACGAAGAAAGGCAAGAAAAAAGTGTAATGCTTTACAAGAACAAAATGTGGGGAATAAAATTTCCACTGTGAAGTCATTGCAGTTTGATTTTGCTACAATTGAAGCAGCCACAAACCAGTTCTCTGTTAATAACAGATTAGGGGAAGGTGGCTTTGGAGAGGTTTACAAGGTAATTATGATGTTTGATTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr Stress-antifung

Protein Analysis

343

Amino Acids

37.61

Weight (kDa)

8.32

Isoelectric Point (pI)

44.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stress-antifung PF01657 28 - 127 8e-25 Salt stress response/antifungal
Stress-antifung PF01657 154 - 226 4e-10 Salt stress response/antifungal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000283)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G45860 AT4G05200 AT4G05200 AT4G11521 AT4G11530 AT4G23130 AT4G23130 AT4G23130 AT4G23140 AT4G23140 AT4G23150 AT4G23160 AT4G23170 AT4G23180 AT4G23180 AT4G23180 AT4G23230 AT4G23270 AT4G23270 AT4G23270 AT4G23280 AT4G23280 AT4G23280 AT4G23310 AT4G23310
fragaria_vesca FvH4_3g02772 FvH4_3g02772 FvH4_3g02773 FvH4_3g02773 FvH4_3g02774 FvH4_3g02800 FvH4_3g02820 FvH4_3g02820 FvH4_3g02830
malus_domestica MD00G1101900.v1.1 MD00G1102100.v1.1 MD00G1102200.v1.1 MD01G1098200.v1.1 MD05G1340000.v1.1 MD05G1340300.v1.1 MD07G1014100.v1.1 MD07G1014300.v1.1 MD07G1014400.v1.1 MD07G1014700.v1.1 MD10G1312900.v1.1 MD11G1022600.v1.1 MD11G1291500.v1.1 MD14G1207100.v1.1 MD16G1096600.v1.1
prunus_persica Prupe.4G027300_v2.0.a1 Prupe.4G027400_v2.0.a1 Prupe.4G027700_v2.0.a1 Prupe.4G027800_v2.0.a1
pyrus_communis pycom05g30960 pycom10g00940 pycom10g00950 pycom10g26430 pycom10g26450 pycom10g26460 pycom10g26470 pycom10g26480 pycom10g26490 pycom10g26500 pycom10g26510 pycom10g26570 pycom11g01820 pycom11g04000 pycom14g17130 pycom14g17140 pycom15g28820
rosa_chinensis RchiOBHm_Chr4g0408141 RchiOBHm_Chr5g0004211 RchiOBHm_Chr5g0004231 RchiOBHm_Chr5g0004241 RchiOBHm_Chr5g0004261
rosa_laevigata RLG00000008668 RLG00000008672 RLG00000031212 RLG00000031217
rosa_multiflora Rmu_co8162658.1_g000001 Rmu_co8336297.1_g000001 Rmu_sc0002073.1_g000001 Rmu_sc0004964.1_g000002 Rmu_sc0004964.1_g000003 Rmu_sc0004964.1_g000007 Rmu_sc0006006.1_g000007 Rmu_sc0007868.1_g000015 Rmu_sc0007868.1_g000016 Rmu_sc0007868.1_g000017 Rmu_sc0007868.1_g000021 Rmu_sc0007868.1_g000023 Rmu_sc0019654.1_g000002 Rmu_sc0038540.1_g000001
rosa_roxburghii Rroxscaffold_1G00071450 Rroxscaffold_1G00071460 Rroxscaffold_1G00071470 Rroxscaffold_1G00071560 Rroxscaffold_1G00071720 Rroxscaffold_1G00071770 Rroxscaffold_5G00352640
rosa_rugosa Rorug04G0083700 Rorug04G0410700 Rorug04G0410800 Rorug04G0410900
rosa_samantha Rh4BG145400 Rh4CG154900 Rh4DG141900 Rh5BG037500 Rh5BG037600 Rh5BG037700 Rh5CG041000 Rh5CG041100 Rh5CG041200 Rh5CG041300 Rh5CG041400 Rh5DG037100
rosa_wichuraiana Rw4G012130 Rw5G003450 Rw5G003460 Rw5G003470 Rw5G003480 Rw5G003640 Rw5G003660 Rw5G003670 Rw5G003680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 603
AciI CCGC 1 cut(s) 197
AclWI GGATC 1 cut(s) 401
AcoI YGGCCR 1 cut(s) 786
AcsI RAATTY 4 cut(s) 333, 414, 642, 897
AcuI CTGAAG 2 cut(s) 201, 717
AcvI CACGTG 1 cut(s) 170
AfaI GTAC 3 cut(s) 317, 350, 622
AfiI CCNNNNNNNGG 3 cut(s) 26, 515, 752
AflIII ACRYGT 2 cut(s) 167, 581
AgeI ACCGGT 1 cut(s) 69
AgsI TTSAA 7 cut(s) 50, 87, 339, 391, 465, 532, 941
AjnI CCWGG 1 cut(s) 751
AjuI GAANNNNNNNTTGG 2 cut(s) 975, 1007
AluBI AGCT 2 cut(s) 53, 596
AluI AGCT 2 cut(s) 53, 596
Alw21I GWGCWC 2 cut(s) 18, 551
Alw44I GTGCAC 1 cut(s) 547
AlwI GGATC 1 cut(s) 401
AoxI GGCC 2 cut(s) 3, 786
ApaLI GTGCAC 1 cut(s) 547
ApeKI GCWGC 3 cut(s) 424, 828, 944
ApoI RAATTY 4 cut(s) 333, 414, 642, 897
ArsI GACNNNNNNTTYG 2 cut(s) 291, 323
AsiGI ACCGGT 1 cut(s) 69
AspLEI GCGC 2 cut(s) 369, 604
AspS9I GGNCC 1 cut(s) 4
AsuHPI GGTGA 2 cut(s) 250, 705
AsuNHI GCTAGC 1 cut(s) 427
BaeGI GKGCMC 1 cut(s) 551
BalI TGGCCA 1 cut(s) 788
BbrPI CACGTG 1 cut(s) 170
BbsI GAAGAC 1 cut(s) 578
Bbv12I GWGCWC 2 cut(s) 18, 551
BbvI GCAGC 3 cut(s) 411, 815, 956
BccI CCATC 3 cut(s) 296, 347, 481
BceAI ACGGC 1 cut(s) 231
BciT130I CCWGG 1 cut(s) 753
BfaI CTAG 3 cut(s) 428, 639, 654
BfmI CTRYAG 1 cut(s) 291
BglII AGATCT 1 cut(s) 253
BisI GCNGC 3 cut(s) 425, 829, 945
BlsI GCNGC 3 cut(s) 426, 830, 946
BmcAI AGTACT 1 cut(s) 317
Bme1390I CCNGG 1 cut(s) 753
BmgT120I GGNCC 1 cut(s) 4
BmrFI CCNGG 1 cut(s) 753
BmtI GCTAGC 1 cut(s) 431
BpiI GAAGAC 1 cut(s) 578
BpmI CTGGAG 1 cut(s) 735
BpuEI CTTGAG 1 cut(s) 39
BsaAI YACGTR 1 cut(s) 170
BsaJI CCNNGG 2 cut(s) 247, 324
BsaWI WCCGGW 1 cut(s) 69
BsaXI ACNNNNNCTCC 2 cut(s) 724, 754
Bsc4I CCNNNNNNNGG 3 cut(s) 26, 515, 752
Bse118I RCCGGY 1 cut(s) 69
Bse1I ACTGG 4 cut(s) 96, 121, 628, 955
Bse3DI GCAATG 1 cut(s) 914
BseBI CCWGG 1 cut(s) 753
BseDI CCNNGG 2 cut(s) 247, 324
BseGI GGATG 2 cut(s) 155, 770
BseLI CCNNNNNNNGG 3 cut(s) 26, 515, 752
BseMI GCAATG 1 cut(s) 914
BseNI ACTGG 4 cut(s) 96, 121, 628, 955
BseRI GAGGAG 1 cut(s) 131
BseSI GKGCMC 1 cut(s) 551
BseXI GCAGC 3 cut(s) 411, 815, 956
BshFI GGCC 2 cut(s) 5, 788
BshTI ACCGGT 1 cut(s) 69
BsiHKAI GWGCWC 2 cut(s) 18, 551
BsiSI CCGG 1 cut(s) 70
BslI CCNNNNNNNGG 3 cut(s) 26, 515, 752
BsnI GGCC 2 cut(s) 5, 788
Bsp1286I GDGCHC 2 cut(s) 18, 551
Bsp143I GATC 2 cut(s) 253, 406
BspACI CCGC 1 cut(s) 197
BspANI GGCC 2 cut(s) 5, 788
BspOI GCTAGC 1 cut(s) 431
BspPI GGATC 1 cut(s) 401
BsrDI GCAATG 1 cut(s) 914
BsrFI RCCGGY 1 cut(s) 69
BsrI ACTGG 4 cut(s) 96, 121, 628, 955
BssAI RCCGGY 1 cut(s) 69
BssECI CCNNGG 2 cut(s) 247, 324
BssMI GATC 2 cut(s) 253, 406
BssT1I CCWWGG 1 cut(s) 324
Bst2UI CCWGG 1 cut(s) 753
Bst4CI ACNGT 2 cut(s) 233, 907
Bst6I CTCTTC 2 cut(s) 151, 741
BstBAI YACGTR 1 cut(s) 170
BstC8I GCNNGC 3 cut(s) 199, 203, 429
BstDEI CTNAG 1 cut(s) 35
BstDSI CCRYGG 1 cut(s) 247
BstENI CCTNNNNNAGG 1 cut(s) 513
BstF5I GGATG 2 cut(s) 155, 770
BstHHI GCGC 2 cut(s) 369, 604
BstKTI GATC 2 cut(s) 256, 409
BstMBI GATC 2 cut(s) 253, 406
BstMWI GCNNNNNNNGC 2 cut(s) 366, 467
BstNI CCWGG 1 cut(s) 753
BstNSI RCATGY 2 cut(s) 364, 585
BstSCI CCNGG 1 cut(s) 751
BstSFI CTRYAG 1 cut(s) 291
BstSLI GKGCMC 1 cut(s) 551
BstV1I GCAGC 3 cut(s) 411, 815, 956
BstV2I GAAGAC 1 cut(s) 578
BstX2I RGATCY 2 cut(s) 253, 406
BstYI RGATCY 2 cut(s) 253, 406
BsuRI GGCC 2 cut(s) 5, 788
BtgI CCRYGG 1 cut(s) 247
BtsCI GGATG 2 cut(s) 155, 770
BtsIMutI CAGTG 2 cut(s) 551, 903
Cac8I GCNNGC 3 cut(s) 199, 203, 429
CfoI GCGC 2 cut(s) 369, 604
Cfr10I RCCGGY 1 cut(s) 69
Cfr13I GGNCC 1 cut(s) 4
Csp6I GTAC 3 cut(s) 316, 349, 621
CspAI ACCGGT 1 cut(s) 69
CviAII CATG 3 cut(s) 361, 421, 582
CviQI GTAC 3 cut(s) 316, 349, 621
DdeI CTNAG 1 cut(s) 35
DpnI GATC 2 cut(s) 255, 408
DpnII GATC 2 cut(s) 253, 406
EaeI YGGCCR 1 cut(s) 786
Eam1104I CTCTTC 2 cut(s) 151, 741
EarI CTCTTC 2 cut(s) 151, 741
Eco130I CCWWGG 1 cut(s) 324
Eco32I GATATC 1 cut(s) 175
Eco57I CTGAAG 2 cut(s) 201, 717
Eco72I CACGTG 1 cut(s) 170
EcoNI CCTNNNNNAGG 1 cut(s) 513
EcoRII CCWGG 1 cut(s) 751
EcoRV GATATC 1 cut(s) 175
EcoT14I CCWWGG 1 cut(s) 324
ErhI CCWWGG 1 cut(s) 324
FaeI CATG 3 cut(s) 364, 424, 585
FatI CATG 3 cut(s) 360, 420, 581
FauI CCCGC 1 cut(s) 190
Fnu4HI GCNGC 3 cut(s) 425, 829, 945
FokI GGATG 2 cut(s) 142, 757
Fsp4HI GCNGC 3 cut(s) 425, 829, 945
FspBI CTAG 3 cut(s) 428, 639, 654
FspI TGCGCA 1 cut(s) 603
GlaI GCGC 2 cut(s) 368, 603
GluI GCNGC 3 cut(s) 425, 829, 945
GsuI CTGGAG 1 cut(s) 735
HaeIII GGCC 2 cut(s) 5, 788
HapII CCGG 1 cut(s) 70
HhaI GCGC 2 cut(s) 369, 604
Hin1II CATG 3 cut(s) 364, 424, 585
Hin6I GCGC 2 cut(s) 367, 602
HinP1I GCGC 2 cut(s) 367, 602
HinfI GANTC 3 cut(s) 180, 733, 1025
HpaII CCGG 1 cut(s) 70
HphI GGTGA 2 cut(s) 250, 705
Hpy166II GTNNAC 3 cut(s) 229, 549, 1003
Hpy188I TCNGA 3 cut(s) 179, 220, 732
Hpy188III TCNNGA 2 cut(s) 388, 590
Hpy8I GTNNAC 3 cut(s) 229, 549, 1003
HpyAV CCTTC 2 cut(s) 720, 977
HpyCH4III ACNGT 2 cut(s) 233, 907
HpyCH4IV ACGT 1 cut(s) 169
HpyCH4V TGCA 6 cut(s) 205, 360, 470, 494, 549, 919
HpyF10VI GCNNNNNNNGC 2 cut(s) 366, 467
HpyF3I CTNAG 1 cut(s) 35
HpySE526I ACGT 1 cut(s) 169
Hsp92II CATG 3 cut(s) 364, 424, 585
HspAI GCGC 2 cut(s) 367, 602
Kzo9I GATC 2 cut(s) 253, 406
LmnI GCTCC 1 cut(s) 21
Lsp1109I GCAGC 3 cut(s) 411, 815, 956
MaeI CTAG 3 cut(s) 428, 639, 654
MaeII ACGT 1 cut(s) 169
MaeIII GTNAC 1 cut(s) 703
MalI GATC 2 cut(s) 255, 408
MboI GATC 2 cut(s) 253, 406
MboII GAAGA 5 cut(s) 128, 138, 578, 728, 855
MfeI CAATTG 1 cut(s) 936
MflI RGATCY 2 cut(s) 253, 406
MhlI GDGCHC 2 cut(s) 18, 551
MlsI TGGCCA 1 cut(s) 788
MluCI AATT 7 cut(s) 333, 414, 642, 801, 897, 936, 1011
MluNI TGGCCA 1 cut(s) 788
MlyI GAGTC 1 cut(s) 727
MmeI TCCRAC 3 cut(s) 183, 378, 755
MnlI CCTC 7 cut(s) 152, 420, 728, 738, 750, 759, 991
Mox20I TGGCCA 1 cut(s) 788
MscI TGGCCA 1 cut(s) 788
MseI TTAA 3 cut(s) 132, 776, 966
Msp20I TGGCCA 1 cut(s) 788
MspI CCGG 1 cut(s) 70
MspR9I CCNGG 1 cut(s) 753
MunI CAATTG 1 cut(s) 936
MvaI CCWGG 1 cut(s) 753
MwoI GCNNNNNNNGC 2 cut(s) 366, 467
NdeII GATC 2 cut(s) 253, 406
NheI GCTAGC 1 cut(s) 427
NlaIII CATG 3 cut(s) 364, 424, 585
NmuCI GTSAC 1 cut(s) 703
NsbI TGCGCA 1 cut(s) 603
NspI RCATGY 2 cut(s) 364, 585
PciI ACATGT 1 cut(s) 581
PfeI GAWTC 2 cut(s) 180, 1025
PfoI TCCNGGA 1 cut(s) 751
PinAI ACCGGT 1 cut(s) 69
PkrI GCNGC 3 cut(s) 426, 830, 946
PleI GAGTC 1 cut(s) 727
PmaCI CACGTG 1 cut(s) 170
PmlI CACGTG 1 cut(s) 170
PpsI GAGTC 1 cut(s) 727
Ppu21I YACGTR 1 cut(s) 170
PscI ACATGT 1 cut(s) 581
Psp6I CCWGG 1 cut(s) 751
PspCI CACGTG 1 cut(s) 170
PspGI CCWGG 1 cut(s) 751
PspPI GGNCC 1 cut(s) 4
PsuI RGATCY 2 cut(s) 253, 406
RsaI GTAC 3 cut(s) 317, 350, 622
RsaNI GTAC 3 cut(s) 316, 349, 621
SaqAI TTAA 3 cut(s) 132, 776, 966
SatI GCNGC 3 cut(s) 425, 829, 945
Sau3AI GATC 2 cut(s) 253, 406
Sau96I GGNCC 1 cut(s) 4
ScaI AGTACT 1 cut(s) 317
SchI GAGTC 1 cut(s) 727
ScrFI CCNGG 1 cut(s) 753
SduI GDGCHC 2 cut(s) 18, 551
SfcI CTRYAG 1 cut(s) 291
SmlI CTYRAG 1 cut(s) 54
SmoI CTYRAG 1 cut(s) 54
Sse9I AATT 7 cut(s) 333, 414, 642, 801, 897, 936, 1011
SsiI CCGC 1 cut(s) 197
SspMI CTAG 3 cut(s) 428, 639, 654
StyD4I CCNGG 1 cut(s) 751
StyI CCWWGG 1 cut(s) 324
TaaI ACNGT 2 cut(s) 233, 907
TaiI ACGT 1 cut(s) 172
TaqI TCGA 2 cut(s) 589, 667
TasI AATT 7 cut(s) 333, 414, 642, 801, 897, 936, 1011
TatI WGTACW 2 cut(s) 315, 620
TfiI GAWTC 2 cut(s) 180, 1025
Tru1I TTAA 3 cut(s) 132, 776, 966
Tru9I TTAA 3 cut(s) 132, 776, 966
TscAI CASTG 2 cut(s) 551, 910
TseFI GTSAC 1 cut(s) 703
TseI GCWGC 3 cut(s) 424, 828, 944
Tsp45I GTSAC 1 cut(s) 703
TspDTI ATGAA 1 cut(s) 1017
TspRI CASTG 2 cut(s) 551, 910
VneI GTGCAC 1 cut(s) 547
XagI CCTNNNNNAGG 1 cut(s) 513
XapI RAATTY 4 cut(s) 333, 414, 642, 897
XceI RCATGY 2 cut(s) 364, 585
XspI CTAG 3 cut(s) 428, 639, 654
ZrmI AGTACT 1 cut(s) 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.