Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0000283

Physical Location & Seq

Genomic Coordinates Reverse (-)

6738146 .. 6741138

Gene Structure 2993 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1096600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2007 bp

ATGAATCACCAAAAAATGAAAAGCATGACTACCCTTCTTTTGATTTTCTCCATTTTTACTCTGCCCAGCTTTCTGACCAATACGGAAGCAGTATACCTTTCCCACATCTGCCCAAACACCACCACCTTCACCCCAAACTCCACCTTCCAGTCCAACCTCAACCGTCTCCTCTCCAACCTCTCGTCCAACGCCAACCGCTCCACCGGCTTCTACAACGTCACCGTCCAAACCACAAACAACGCCGTCTACGGCCTCTTCCTCTGCCGCGGCGACGTCGTGAGCACCGACGCTTGCGAAACCTGTGTAGCCACCGCAACCACCGAGGCCCCACAACGCTGCCCCGTAGAAAAACAGGTGGTCATCTGGTACGACGACTGCATGCTACGCTACTCCAACGTTTCTTTCTTCTCCACCGTCACCGAGTCGCCCCGCTGGTACATGTGGAACACGCAGAACGCCACCGACCCGACTCGGTTCAACCAGGTCATGGCGGCGAGTGTGAACCAGGCGGCCAGTGAGGCTGTCGGAGACGCCGACAAGTTCGGGACGAATCAGGCAAATGTTAGTGGGTTGATTTCGGTGTACAGCCTCGGTCAGTGCACGCAAGACCTGTCGGCGACGGATTGCAACCGCTGTCTTCGGGGGACCATAGCCCTACTTCCGAGTTGCTGTAATGGGGCACTCGGAGCACGAGTTTTGTATCCGAGTTGTAATGTTAGGTACGAAGTCTACCCCTTCTACGCCCAGAACGCCACGTCAGCACCTGAGCCTGCACCAGGGCCGCCTCCTCCTCTTCCTCCTCCGACTAAAGGAAAAAGTAAAATCCCAACTAGGACAATTGTTGCCATTGTTGTACCAATATCTGTATCTGTGCTACTTTTTGTTGTGGGTTACTGCTGCATAACTAGGAGAGCAAGAAAGAAGTACAATCAAGCAGCAGCAGACGAACCAAGCGGTGAGAATGACATTACTACTGTCGAGTCTTTGCAATTTGATTTTGCTACCATCCAAGCAGCTACGAGCAATTTCTCCGACGATCACAAGTTAGGCGAAGGCGGTTTCGGTCAAGTTTACAAGGGTACACTTTCAAACGGTCAAGAAGTAGCTGTGAAGAGGCTATCAAGGAACTCTGGCCAAGGCACAGAAGAGTTTAAGAACGAGATGGTATTAGTAGCCAAGCTTCAACACCGAAATTTGGTTAGGCTATTGGGATTTTGCTTGGAAGGCGAAGAAAAGATTCTTGTTTATGAATATGTGCTCAACAAAAGCCTCGACTGTTTCCTATTTGACCCTGAGAAACAAGGACAATTGGATTGGTCAAGACGTTACAAGATTATTTCAGGAATTGCTCGAGGAATCATGTACCTGCATGAAGATTCCCGACTTAGAATTATACATCGTGATCTCAAAGCCAGCAATATATTGTTAGATGGGGAGATGCATCCAAAAATATCTGATTTTGGTATGGCCAGGATCTTTGGGGTTGATCAAACTCAAGCAAACACAAATAGAATTGTCGGAACATATGGCTATATGTCTCCTGAGTATGCAATGCACGGGCATTTTTCTGTCAAGTCCGATCTGTTTAGTTTCGGTGTTTTAGTGCTAGAAATCATTAGTGGCAAGAAGAACAGTAACTTCTTTCAAACCGATGCAGCTGAGGACCTCGCGAGCCATGCTTGGAAGTTATGGAGAGACGGGACATCTTTGGAATTGTTGGATCAGTGTCTGAGAGACTCTTATTCAAGGACTGAAGTAATCAGATGCATCCACATCGGGTTACTTTGTGTTCAGGAAGATCCAGCTGACAGGCCTACAATGCAATCAGTAGTTCTCATGCTGAATAGCTACTCTGTCAGTCTGCCATTACCTAAACAACCGGCATTTTTTCTCCAAAGCAGAACAGTGGGGAACATGCCAATGATAACGCTGGAGTCCGATCAATCTATCAGCAAGTCATCTCCGTCCGTTAATGAAGGATCTATCACTGAAGTATACCCTAGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0042742 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050896 GO:0051704 GO:0051707 GO:0071704 GO:0071944 GO:0098542 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr Stress-antifung

Protein Analysis

669

Amino Acids

74.06

Weight (kDa)

6.44

Isoelectric Point (pI)

44.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Stress-antifung	PF01657	37 - 132	2.6e-27	Salt stress response/antifungal
Stress-antifung	PF01657	151 - 243	2e-11	Salt stress response/antifungal
Pkinase	PF00069	344 - 611	1.6e-45	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	346 - 612	4.4e-49	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0000283)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G45860 AT4G05200 AT4G05200 AT4G11521 AT4G11530 AT4G23130 AT4G23130 AT4G23130 AT4G23140 AT4G23140 AT4G23150 AT4G23160 AT4G23170 AT4G23180 AT4G23180 AT4G23180 AT4G23230 AT4G23270 AT4G23270 AT4G23270 AT4G23280 AT4G23280 AT4G23280 AT4G23310 AT4G23310
fragaria_vesca	FvH4_3g02772 FvH4_3g02772 FvH4_3g02773 FvH4_3g02773 FvH4_3g02774 FvH4_3g02800 FvH4_3g02820 FvH4_3g02820 FvH4_3g02830
malus_domestica	MD00G1101900.v1.1 MD00G1102100.v1.1 MD00G1102200.v1.1 MD01G1098200.v1.1 MD05G1340000.v1.1 MD05G1340300.v1.1 MD07G1014100.v1.1 MD07G1014300.v1.1 MD07G1014400.v1.1 MD07G1014700.v1.1 MD10G1312900.v1.1 MD11G1022600.v1.1 MD11G1291500.v1.1 MD14G1207100.v1.1 MD16G1096600.v1.1
prunus_persica	Prupe.4G027300_v2.0.a1 Prupe.4G027400_v2.0.a1 Prupe.4G027700_v2.0.a1 Prupe.4G027800_v2.0.a1
pyrus_communis	pycom05g30960 pycom10g00940 pycom10g00950 pycom10g26430 pycom10g26450 pycom10g26460 pycom10g26470 pycom10g26480 pycom10g26490 pycom10g26500 pycom10g26510 pycom10g26570 pycom11g01820 pycom11g04000 pycom14g17130 pycom14g17140 pycom15g28820
rosa_chinensis	RchiOBHm_Chr4g0408141 RchiOBHm_Chr5g0004211 RchiOBHm_Chr5g0004231 RchiOBHm_Chr5g0004241 RchiOBHm_Chr5g0004261
rosa_laevigata	RLG00000008668 RLG00000008672 RLG00000031212 RLG00000031217
rosa_multiflora	Rmu_co8162658.1_g000001 Rmu_co8336297.1_g000001 Rmu_sc0002073.1_g000001 Rmu_sc0004964.1_g000002 Rmu_sc0004964.1_g000003 Rmu_sc0004964.1_g000007 Rmu_sc0006006.1_g000007 Rmu_sc0007868.1_g000015 Rmu_sc0007868.1_g000016 Rmu_sc0007868.1_g000017 Rmu_sc0007868.1_g000021 Rmu_sc0007868.1_g000023 Rmu_sc0019654.1_g000002 Rmu_sc0038540.1_g000001
rosa_roxburghii	Rroxscaffold_1G00071450 Rroxscaffold_1G00071460 Rroxscaffold_1G00071470 Rroxscaffold_1G00071560 Rroxscaffold_1G00071720 Rroxscaffold_1G00071770 Rroxscaffold_5G00352640
rosa_rugosa	Rorug04G0083700 Rorug04G0410700 Rorug04G0410800 Rorug04G0410900
rosa_samantha	Rh4BG145400 Rh4CG154900 Rh4DG141900 Rh5BG037500 Rh5BG037600 Rh5BG037700 Rh5CG041000 Rh5CG041100 Rh5CG041200 Rh5CG041300 Rh5CG041400 Rh5DG037100
rosa_wichuraiana	Rw4G012130 Rw5G003450 Rw5G003460 Rw5G003470 Rw5G003480 Rw5G003640 Rw5G003660 Rw5G003670 Rw5G003680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	276
Acc36I	ACCTGC	1 cut(s)	1374
AccB7I	CCANNNNNTGG	1 cut(s)	487
AccBSI	CCGCTC	1 cut(s)	198
AccI	GTMKAC	4 cut(s)	93, 246, 729, 1995
AccII	CGCG	2 cut(s)	267, 1670
AclI	AACGTT	1 cut(s)	396
AclWI	GGATC	4 cut(s)	1481, 1728, 1793, 1987
AcoI	YGGCCR	3 cut(s)	510, 1132, 1467
AcsI	RAATTY	1 cut(s)	1192
AcuI	CTGAAG	1 cut(s)	1773
AcyI	GRCGYC	2 cut(s)	273, 531
AfaI	GTAC	8 cut(s)	368, 437, 584, 722, 855, 926, 1081, 1364
AfiI	CCNNNNNNNGG	4 cut(s)	487, 776, 809, 1195
AflIII	ACRYGT	1 cut(s)	438
AgsI	TTSAA	5 cut(s)	478, 1089, 1184, 1646, 1746
AjiI	CACGTC	1 cut(s)	756
AjnI	CCWGG	4 cut(s)	480, 504, 775, 1469
AjuI	GAANNNNNNNTTGG	2 cut(s)	386, 418
AluBI	AGCT	7 cut(s)	69, 1016, 1106, 1180, 1658, 1805, 1848
AluI	AGCT	7 cut(s)	69, 1016, 1106, 1180, 1658, 1805, 1848
Alw21I	GWGCWC	4 cut(s)	284, 602, 691, 1260
Alw26I	GTCTC	5 cut(s)	170, 522, 1542, 1689, 1728
Alw44I	GTGCAC	1 cut(s)	598
AlwI	GGATC	4 cut(s)	1481, 1728, 1793, 1987
AlwNI	CAGNNNCTG	2 cut(s)	764, 1729
Ama87I	CYCGRG	1 cut(s)	1350
AoxI	GGCC	7 cut(s)	250, 324, 510, 779, 1132, 1467, 1811
ApaLI	GTGCAC	1 cut(s)	598
ApeKI	GCWGC	6 cut(s)	336, 897, 935, 938, 1013, 1655
ApoI	RAATTY	1 cut(s)	1192
Asp700I	GAANNNNTTC	1 cut(s)	1236
AspS9I	GGNCC	4 cut(s)	325, 645, 779, 1663
AsuHPI	GGTGA	4 cut(s)	121, 211, 409, 968
AvaI	CYCGRG	1 cut(s)	1350
AvaII	GGWCC	2 cut(s)	645, 1663
BaeGI	GKGCMC	2 cut(s)	602, 682
BalI	TGGCCA	2 cut(s)	1134, 1469
BarI	GAAGNNNNNNTAC	2 cut(s)	1164, 1196
BauI	CACGAG	1 cut(s)	690
BbsI	GAAGAC	1 cut(s)	629
Bbv12I	GWGCWC	4 cut(s)	284, 602, 691, 1260
BbvCI	CCTCAGC	1 cut(s)	1659
BbvI	GCAGC	6 cut(s)	323, 884, 947, 950, 1025, 1667
BccI	CCATC	3 cut(s)	1013, 1156, 1424
BceAI	ACGGC	2 cut(s)	227, 265
BcgI	CGANNNNNNTGC	2 cut(s)	1756, 1790
BciT130I	CCWGG	4 cut(s)	482, 506, 777, 1471
BciVI	GTATCC	1 cut(s)	711
BclI	TGATCA	1 cut(s)	1486
BcoDI	GTCTC	5 cut(s)	170, 522, 1542, 1689, 1728
BfaI	CTAG	4 cut(s)	831, 906, 1607, 2001
BfuAI	ACCTGC	1 cut(s)	1374
BfuI	GTATCC	1 cut(s)	711
BglI	GCCNNNNNGGC	1 cut(s)	518
Bme1390I	CCNGG	4 cut(s)	482, 506, 777, 1471
Bme18I	GGWCC	2 cut(s)	645, 1663
BmeT110I	CYCGRG	1 cut(s)	1350
BmgBI	CACGTC	1 cut(s)	756
BmgT120I	GGNCC	4 cut(s)	325, 645, 779, 1663
BmiI	GGNNCC	2 cut(s)	327, 646
BmrFI	CCNGG	4 cut(s)	482, 506, 777, 1471
BmsI	GCATC	5 cut(s)	1428, 1450, 1642, 1754, 1776
BpiI	GAAGAC	1 cut(s)	629
BpmI	CTGGAG	1 cut(s)	1952
Bpu10I	CCTNAGC	2 cut(s)	765, 1659
BpuEI	CTTGAG	1 cut(s)	1479
BsaHI	GRCGYC	2 cut(s)	273, 531
BsaJI	CCNNGG	5 cut(s)	265, 321, 589, 776, 1135
BsaXI	ACNNNNNCTCC	2 cut(s)	678, 708
Bsc4I	CCNNNNNNNGG	4 cut(s)	487, 776, 809, 1195
Bse118I	RCCGGY	2 cut(s)	203, 1879
Bse1I	ACTGG	2 cut(s)	148, 513
Bse3DI	GCAATG	1 cut(s)	1557
BseBI	CCWGG	4 cut(s)	482, 506, 777, 1471
BseDI	CCNNGG	5 cut(s)	265, 321, 589, 776, 1135
BseGI	GGATG	3 cut(s)	1005, 1441, 1767
BseLI	CCNNNNNNNGG	4 cut(s)	487, 776, 809, 1195
BseMI	GCAATG	1 cut(s)	1557
BseMII	CTCAG	5 cut(s)	756, 1284, 1533, 1650, 1721
BseNI	ACTGG	2 cut(s)	148, 513
BseRI	GAGGAG	4 cut(s)	158, 777, 780, 789
BseSI	GKGCMC	2 cut(s)	602, 682
BseXI	GCAGC	6 cut(s)	323, 884, 947, 950, 1025, 1667
BseYI	CCCAGC	1 cut(s)	65
BsgI	GTGCAG	1 cut(s)	756
Bsh1236I	CGCG	2 cut(s)	267, 1670
BshFI	GGCC	7 cut(s)	252, 326, 512, 781, 1134, 1469, 1813
BsiHKAI	GWGCWC	4 cut(s)	284, 602, 691, 1260
BsiHKCI	CYCGRG	1 cut(s)	1350
BsiSI	CCGG	2 cut(s)	204, 1880
BslFI	GGGAC	3 cut(s)	559, 658, 1714
BslI	CCNNNNNNNGG	4 cut(s)	487, 776, 809, 1195
BsmAI	GTCTC	5 cut(s)	170, 522, 1542, 1689, 1728
BsmBI	CGTCTC	3 cut(s)	170, 522, 1689
BsmFI	GGGAC	3 cut(s)	559, 658, 1714
BsnI	GGCC	7 cut(s)	252, 326, 512, 781, 1134, 1469, 1813
BsoBI	CYCGRG	1 cut(s)	1350
Bsp1286I	GDGCHC	5 cut(s)	284, 602, 682, 691, 1260
Bsp1407I	TGTACA	1 cut(s)	582
Bsp143I	GATC	9 cut(s)	1036, 1402, 1473, 1486, 1579, 1720, 1798, 1939, 1979
Bsp68I	TCGCGA	1 cut(s)	1670
BspANI	GGCC	7 cut(s)	252, 326, 512, 781, 1134, 1469, 1813
BspCNI	CTCAG	5 cut(s)	757, 1285, 1534, 1651, 1722
BspFNI	CGCG	2 cut(s)	267, 1670
BspLI	GGNNCC	2 cut(s)	327, 646
BspMI	ACCTGC	1 cut(s)	1374
BspPI	GGATC	4 cut(s)	1481, 1728, 1793, 1987
BsrBI	CCGCTC	1 cut(s)	198
BsrDI	GCAATG	1 cut(s)	1557
BsrFI	RCCGGY	2 cut(s)	203, 1879
BsrGI	TGTACA	1 cut(s)	582
BsrI	ACTGG	2 cut(s)	148, 513
BssAI	RCCGGY	2 cut(s)	203, 1879
BssECI	CCNNGG	5 cut(s)	265, 321, 589, 776, 1135
BssMI	GATC	9 cut(s)	1036, 1402, 1473, 1486, 1579, 1720, 1798, 1939, 1979
BssNAI	GTATAC	2 cut(s)	94, 1996
BssNI	GRCGYC	2 cut(s)	273, 531
BssSI	CACGAG	1 cut(s)	690
BssT1I	CCWWGG	1 cut(s)	1135
Bst1107I	GTATAC	2 cut(s)	94, 1996
Bst2BI	CACGAG	1 cut(s)	690
Bst2UI	CCWGG	4 cut(s)	482, 506, 777, 1471
Bst4CI	ACNGT	8 cut(s)	164, 223, 415, 976, 1094, 1277, 1634, 1906
Bst6I	CTCTTC	4 cut(s)	260, 798, 1106, 1140
BstACI	GRCGYC	2 cut(s)	273, 531
BstAUI	TGTACA	1 cut(s)	582
BstC8I	GCNNGC	6 cut(s)	292, 380, 602, 771, 1414, 1672
BstDEI	CTNAG	6 cut(s)	765, 1293, 1385, 1542, 1659, 1730
BstDSI	CCRYGG	1 cut(s)	265
BstENI	CCTNNNNNAGG	1 cut(s)	774
BstF5I	GGATG	3 cut(s)	1005, 1441, 1767
BstFNI	CGCG	2 cut(s)	267, 1670
BstKTI	GATC	9 cut(s)	1039, 1405, 1476, 1489, 1582, 1723, 1801, 1942, 1982
BstMAI	GTCTC	5 cut(s)	170, 522, 1542, 1689, 1728
BstMBI	GATC	9 cut(s)	1036, 1402, 1473, 1486, 1579, 1720, 1798, 1939, 1979
BstMWI	GCNNNNNNNGC	9 cut(s)	204, 384, 518, 686, 749, 758, 1224, 1676, 1819
BstNI	CCWGG	4 cut(s)	482, 506, 777, 1471
BstNSI	RCATGY	3 cut(s)	382, 442, 1918
BstSCI	CCNGG	4 cut(s)	480, 504, 775, 1469
BstSLI	GKGCMC	2 cut(s)	602, 682
BstUI	CGCG	2 cut(s)	267, 1670
BstV1I	GCAGC	6 cut(s)	323, 884, 947, 950, 1025, 1667
BstV2I	GAAGAC	1 cut(s)	629
BstX2I	RGATCY	3 cut(s)	1473, 1798, 1979
BstYI	RGATCY	3 cut(s)	1473, 1798, 1979
BstZ17I	GTATAC	2 cut(s)	94, 1996
BsuI	GTATCC	1 cut(s)	711
BsuRI	GGCC	7 cut(s)	252, 326, 512, 781, 1134, 1469, 1813
BtgI	CCRYGG	1 cut(s)	265
BtrI	CACGTC	1 cut(s)	756
BtsCI	GGATG	3 cut(s)	1005, 1441, 1767
BtsIMutI	CAGTG	5 cut(s)	520, 602, 1730, 1911, 1986
BtuMI	TCGCGA	1 cut(s)	1670
BveI	ACCTGC	1 cut(s)	1374
Cac8I	GCNNGC	6 cut(s)	292, 380, 602, 771, 1414, 1672
CaiI	CAGNNNCTG	2 cut(s)	764, 1729
Cfr10I	RCCGGY	2 cut(s)	203, 1879
Cfr13I	GGNCC	4 cut(s)	325, 645, 779, 1663
Cfr42I	CCGCGG	1 cut(s)	268
CseI	GACGC	2 cut(s)	296, 539
CsiI	ACCWGGT	1 cut(s)	480
Csp6I	GTAC	8 cut(s)	367, 436, 583, 721, 854, 925, 1080, 1363
CviAII	CATG	9 cut(s)	25, 379, 439, 487, 1360, 1370, 1676, 1837, 1915
CviQI	GTAC	8 cut(s)	367, 436, 583, 721, 854, 925, 1080, 1363
DdeI	CTNAG	6 cut(s)	765, 1293, 1385, 1542, 1659, 1730
DpnI	GATC	9 cut(s)	1038, 1404, 1475, 1488, 1581, 1722, 1800, 1941, 1981
DpnII	GATC	9 cut(s)	1036, 1402, 1473, 1486, 1579, 1720, 1798, 1939, 1979
EaeI	YGGCCR	3 cut(s)	510, 1132, 1467
Eam1104I	CTCTTC	4 cut(s)	260, 798, 1106, 1140
EarI	CTCTTC	4 cut(s)	260, 798, 1106, 1140
Eco130I	CCWWGG	1 cut(s)	1135
Eco147I	AGGCCT	1 cut(s)	1813
Eco47I	GGWCC	2 cut(s)	645, 1663
Eco57I	CTGAAG	1 cut(s)	1773
Eco88I	CYCGRG	1 cut(s)	1350
EcoNI	CCTNNNNNAGG	1 cut(s)	774
EcoO109I	RGGNCCY	2 cut(s)	325, 1663
EcoRII	CCWGG	4 cut(s)	480, 504, 775, 1469
EcoT14I	CCWWGG	1 cut(s)	1135
EcoT22I	ATGCAT	2 cut(s)	1443, 1769
ErhI	CCWWGG	1 cut(s)	1135
Esp3I	CGTCTC	3 cut(s)	170, 522, 1689
FaeI	CATG	9 cut(s)	28, 382, 442, 490, 1363, 1373, 1679, 1840, 1918
FaqI	GGGAC	3 cut(s)	559, 658, 1714
FatI	CATG	9 cut(s)	24, 378, 438, 486, 1359, 1369, 1675, 1836, 1914
FauI	CCCGC	1 cut(s)	437
FauNDI	CATATG	1 cut(s)	1525
FbaI	TGATCA	1 cut(s)	1486
FblI	GTMKAC	4 cut(s)	93, 246, 729, 1995
FokI	GGATG	3 cut(s)	992, 1428, 1754
FspBI	CTAG	4 cut(s)	831, 906, 1607, 2001
GsaI	CCCAGC	1 cut(s)	69
GsuI	CTGGAG	1 cut(s)	1952
HaeIII	GGCC	7 cut(s)	252, 326, 512, 781, 1134, 1469, 1813
HapII	CCGG	2 cut(s)	204, 1880
HgaI	GACGC	2 cut(s)	296, 539
Hin1I	GRCGYC	2 cut(s)	273, 531
Hin1II	CATG	9 cut(s)	28, 382, 442, 490, 1363, 1373, 1679, 1840, 1918
HindIII	AAGCTT	1 cut(s)	1178
HpaII	CCGG	2 cut(s)	204, 1880
HphI	GGTGA	4 cut(s)	121, 211, 409, 968
Hpy166II	GTNNAC	9 cut(s)	94, 247, 502, 583, 600, 730, 1072, 1082, 1996
Hpy8I	GTNNAC	9 cut(s)	94, 247, 502, 583, 600, 730, 1072, 1082, 1996
Hpy99I	CGWCG	6 cut(s)	275, 278, 290, 374, 622, 1037
HpyAV	CCTTC	7 cut(s)	44, 136, 154, 745, 1046, 1217, 1970
HpyCH4III	ACNGT	8 cut(s)	164, 223, 415, 976, 1094, 1277, 1634, 1906
HpyCH4IV	ACGT	5 cut(s)	216, 273, 396, 755, 1324
HpyF10VI	GCNNNNNNNGC	9 cut(s)	204, 384, 518, 686, 749, 758, 1224, 1676, 1819
HpyF3I	CTNAG	6 cut(s)	765, 1293, 1385, 1542, 1659, 1730
HpySE526I	ACGT	5 cut(s)	216, 273, 396, 755, 1324
Hsp92I	GRCGYC	2 cut(s)	273, 531
Hsp92II	CATG	9 cut(s)	28, 382, 442, 490, 1363, 1373, 1679, 1840, 1918
Ksp22I	TGATCA	1 cut(s)	1486
KspI	CCGCGG	1 cut(s)	268
Kzo9I	GATC	9 cut(s)	1036, 1402, 1473, 1486, 1579, 1720, 1798, 1939, 1979
LmnI	GCTCC	2 cut(s)	203, 686
Lsp1109I	GCAGC	6 cut(s)	323, 884, 947, 950, 1025, 1667
LweI	GCATC	5 cut(s)	1428, 1450, 1642, 1754, 1776
MabI	ACCWGGT	1 cut(s)	480
MaeI	CTAG	4 cut(s)	831, 906, 1607, 2001
MaeII	ACGT	5 cut(s)	216, 273, 396, 755, 1324
MaeIII	GTNAC	6 cut(s)	217, 415, 890, 1325, 1634, 1779
MalI	GATC	9 cut(s)	1038, 1404, 1475, 1488, 1581, 1722, 1800, 1941, 1981
MbiI	CCGCTC	1 cut(s)	198
MboI	GATC	9 cut(s)	1036, 1402, 1473, 1486, 1579, 1720, 1798, 1939, 1979
MfeI	CAATTG	2 cut(s)	837, 1307
MflI	RGATCY	3 cut(s)	1473, 1798, 1979
MhlI	GDGCHC	5 cut(s)	284, 602, 682, 691, 1260
MlsI	TGGCCA	2 cut(s)	1134, 1469
MluCI	AATT	9 cut(s)	837, 989, 1024, 1192, 1307, 1344, 1389, 1512, 1712
MluNI	TGGCCA	2 cut(s)	1134, 1469
MlyI	GAGTC	5 cut(s)	431, 463, 989, 1730, 1943
MmeI	TCCRAC	9 cut(s)	177, 198, 210, 417, 505, 827, 1056, 1498, 1698
Mox20I	TGGCCA	2 cut(s)	1134, 1469
Mph1103I	ATGCAT	2 cut(s)	1443, 1769
MroXI	GAANNNNTTC	1 cut(s)	1236
MscI	TGGCCA	2 cut(s)	1134, 1469
MseI	TTAA	2 cut(s)	1152, 1971
MslI	CAYNNNNRTG	1 cut(s)	1919
Msp20I	TGGCCA	2 cut(s)	1134, 1469
MspA1I	CMGCKG	5 cut(s)	267, 432, 633, 1658, 1805
MspI	CCGG	2 cut(s)	204, 1880
MspR9I	CCNGG	4 cut(s)	482, 506, 777, 1471
MunI	CAATTG	2 cut(s)	837, 1307
MvaI	CCWGG	4 cut(s)	482, 506, 777, 1471
MvnI	CGCG	2 cut(s)	267, 1670
MwoI	GCNNNNNNNGC	9 cut(s)	204, 384, 518, 686, 749, 758, 1224, 1676, 1819
NdeI	CATATG	1 cut(s)	1525
NdeII	GATC	9 cut(s)	1036, 1402, 1473, 1486, 1579, 1720, 1798, 1939, 1979
NlaIII	CATG	9 cut(s)	28, 382, 442, 490, 1363, 1373, 1679, 1840, 1918
NlaIV	GGNNCC	2 cut(s)	327, 646
NmuCI	GTSAC	2 cut(s)	217, 415
NruI	TCGCGA	1 cut(s)	1670
NsiI	ATGCAT	2 cut(s)	1443, 1769
NspI	RCATGY	3 cut(s)	382, 442, 1918
PaeI	GCATGC	1 cut(s)	382
PaeR7I	CTCGAG	1 cut(s)	1350
PceI	AGGCCT	1 cut(s)	1813
PciI	ACATGT	1 cut(s)	438
PcsI	WCGNNNNNNNCGW	2 cut(s)	282, 531
PdmI	GAANNNNTTC	1 cut(s)	1236
PfeI	GAWTC	5 cut(s)	4, 550, 1237, 1356, 1376
PflMI	CCANNNNNTGG	1 cut(s)	487
PleI	GAGTC	5 cut(s)	430, 463, 988, 1730, 1942
PpsI	GAGTC	5 cut(s)	430, 463, 988, 1730, 1942
PpuMI	RGGWCCY	1 cut(s)	1663
PscI	ACATGT	1 cut(s)	438
Psp1406I	AACGTT	1 cut(s)	396
Psp5II	RGGWCCY	1 cut(s)	1663
Psp6I	CCWGG	4 cut(s)	480, 504, 775, 1469
PspFI	CCCAGC	1 cut(s)	65
PspGI	CCWGG	4 cut(s)	480, 504, 775, 1469
PspN4I	GGNNCC	2 cut(s)	327, 646
PspPI	GGNCC	4 cut(s)	325, 645, 779, 1663
PspPPI	RGGWCCY	1 cut(s)	1663
PspXI	VCTCGAGB	1 cut(s)	1350
PstNI	CAGNNNCTG	2 cut(s)	764, 1729
PsuI	RGATCY	3 cut(s)	1473, 1798, 1979
PvuII	CAGCTG	2 cut(s)	1658, 1805
RruI	TCGCGA	1 cut(s)	1670
RsaI	GTAC	8 cut(s)	368, 437, 584, 722, 855, 926, 1081, 1364
RsaNI	GTAC	8 cut(s)	367, 436, 583, 721, 854, 925, 1080, 1363
RseI	CAYNNNNRTG	1 cut(s)	1919
SacII	CCGCGG	1 cut(s)	268
SaqAI	TTAA	2 cut(s)	1152, 1971
Sau3AI	GATC	9 cut(s)	1036, 1402, 1473, 1486, 1579, 1720, 1798, 1939, 1979
Sau96I	GGNCC	4 cut(s)	325, 645, 779, 1663
SchI	GAGTC	5 cut(s)	431, 463, 989, 1730, 1943
ScrFI	CCNGG	4 cut(s)	482, 506, 777, 1471
SduI	GDGCHC	5 cut(s)	284, 602, 682, 691, 1260
SexAI	ACCWGGT	1 cut(s)	480
SfaNI	GCATC	5 cut(s)	1428, 1450, 1642, 1754, 1776
Sfr274I	CTCGAG	1 cut(s)	1350
Sfr303I	CCGCGG	1 cut(s)	268
SgrBI	CCGCGG	1 cut(s)	268
SinI	GGWCC	2 cut(s)	645, 1663
SlaI	CTCGAG	1 cut(s)	1350
SmiMI	CAYNNNNRTG	1 cut(s)	1919
SmlI	CTYRAG	2 cut(s)	1350, 1494
SmoI	CTYRAG	2 cut(s)	1350, 1494
SphI	GCATGC	1 cut(s)	382
Sse9I	AATT	9 cut(s)	837, 989, 1024, 1192, 1307, 1344, 1389, 1512, 1712
SseBI	AGGCCT	1 cut(s)	1813
SspMI	CTAG	4 cut(s)	831, 906, 1607, 2001
StuI	AGGCCT	1 cut(s)	1813
StyD4I	CCNGG	4 cut(s)	480, 504, 775, 1469
StyI	CCWWGG	1 cut(s)	1135
TaaI	ACNGT	8 cut(s)	164, 223, 415, 976, 1094, 1277, 1634, 1906
TaiI	ACGT	5 cut(s)	219, 276, 399, 758, 1327
TaqI	TCGA	3 cut(s)	978, 1272, 1351
TaqII	GACCGA	2 cut(s)	581, 1052
TasI	AATT	9 cut(s)	837, 989, 1024, 1192, 1307, 1344, 1389, 1512, 1712
TatI	WGTACW	2 cut(s)	582, 924
TauI	GCSGC	5 cut(s)	267, 270, 494, 512, 784
TfiI	GAWTC	5 cut(s)	4, 550, 1237, 1356, 1376
Tru1I	TTAA	2 cut(s)	1152, 1971
Tru9I	TTAA	2 cut(s)	1152, 1971
TscAI	CASTG	5 cut(s)	520, 602, 1730, 1911, 1993
TseFI	GTSAC	2 cut(s)	217, 415
TseI	GCWGC	6 cut(s)	336, 897, 935, 938, 1013, 1655
Tsp45I	GTSAC	2 cut(s)	217, 415
TspDTI	ATGAA	5 cut(s)	17, 32, 1263, 1386, 1989
TspGWI	ACGGA	4 cut(s)	98, 635, 1953, 1957
TspRI	CASTG	5 cut(s)	520, 602, 1730, 1911, 1993
Van91I	CCANNNNNTGG	1 cut(s)	487
VneI	GTGCAC	1 cut(s)	598
VpaK11BI	GGWCC	2 cut(s)	645, 1663
XagI	CCTNNNNNAGG	1 cut(s)	774
XapI	RAATTY	1 cut(s)	1192
XceI	RCATGY	3 cut(s)	382, 442, 1918
XhoI	CTCGAG	1 cut(s)	1350
XmiI	GTMKAC	4 cut(s)	93, 246, 729, 1995
XmnI	GAANNNNTTC	1 cut(s)	1236
XspI	CTAG	4 cut(s)	831, 906, 1607, 2001
ZraI	GACGTC	1 cut(s)	274
Zsp2I	ATGCAT	2 cut(s)	1443, 1769

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.