RchiOBHm_Chr5g0004231

Cysteine-rich receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000283
Physical Location & Seq
Forward (+)
2684905 .. 2688573
3669 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28549

Sequence Viewer

Length: 1974 bp
ATGGCAGAAGTGATCCTTCTCTTCCTTTCAGTGGTCACCCTGGTAAGCTTTCCGACGACAAAAGCCGACTACCTCTACCATAGCTGCCCCAACACTACCGTCTTCACCCCAAACTCCACCTTCCAGTCCAACCTCAACCACCTCTTCTCTACCCTCTCCTCCAACGCCACACGCGACACCGGCTTCTACAACGCCACCTCCGGCCTTACCCCTAATGAAACCGTCTACGGCCTCTTCCTCTGCCGCGGTGACGTCACCACAGCCGCTTGCCGGTCCTGCGTGACCACCGCAACCTCCGAGGTCGTGCAGCGCTGCCCGGTCGAGAAAGAGGTTGTGATATGGTACGACGACTGCTTGCTCCGCTACTCCAACAAGTCCTTCTTCTCCGTCGCCACTGAGTCGCCGGGGGTGTTCATGTGGAACACGGGTAACATAAGCAGCGAACCGAACCGGTTTAACCAGGTTTTGGCGGCGAGCATGAAGGAGGTGGCCACGGTGGCTTCGAACGACGGCGATAAGTTCGCGACGAGAGAGGCGAACTTCACGGAGTTGATTAGCTTGTACAGCTTAGGGCAGTGCACGCAGGACTTGTCTAGTTTTGATTGCAATCGGTGTCTTCTGGGAGCCATATCGCAGTTGCCGGGTTGCTGCGGCGGGAAGCAAGGGGGACGAGTTCTGTATCCGAGTTGTAATGTTAGGTATGAAGTTTATCCCTTTTATAACTCGACGTCAGCACCGCCGCCGGCGCCAGGGGCTCGACCTCTTTCTCCGCCGAGCACAGTGACGAAAGGTAAGAGCAAAATCTCCTGGAAAAAGATTGTGGCCATTGCCGTTTCAATTGCTGCCTCTTTGGTGCTTTTTGTGATCGGCTACTGCCTCATCACTAAGAGGGCAAAAAAGAAGTACAATGCAGTACCAGAAACAAGTGAGAATGACATTTCTAGTGTAGAGTCTTTGCAATTTGATTTTGGTACCATTGAAGCTGCTACAAACAATTTCTCTGATGATAACAAGTTAGGCGAAGGTGGATTTGGTCAAGTCTACAAGGGTTTGCTTTCTGATGGTCAAGAAATAGCTGTCAAGAGGCTATCAAGGAACTCTGGCCAAGGTGCACAAGAATTTAAGAATGAGATGGTACTGGTAGCCAAGCTTCAACACCGAAATCTGGTCAGGCTATTGGGATTTTGCTTGGAAGGGGAAGAAAAGATGCTTGTTTATGAATACGTCCCCAACAAAAGCCTCGACTATATTCTATTTGACCCTGAGAAACAAGGACAACTGGATTGGTCGAGACGTTACAAAATTATTGCGGGGATTGCCCGAGGAATTATGTATCTACATGAAGACTCCCGGCTAAGAATTATACATCGTGATCTTAAAGCCAGCAATATATTGTTAGATGGGGAAATGCATCCAAAAATATCTGATTTTGGCATGGCGAGGATATTCGGCGTTGATCAAACTCAAGCAAACACAAATAGAATTGTTGGAACATATGGCTACATGTCTCCAGAATATGCAATGCATGGACAATTTTCTATCAAGTCCGATATCTATAGTTTTGGTGTCTTAGTTCTAGAAATTATCAGTGGCAAGAAGAATAGTTACTTCTTCCAAACGAATGCAGATGAGGACCTTGTGAGCCATGCTTGGAAACTATGGAGAGATGGAACTCCTTTGGAATTTTTGGATCCGTGTCTGAGAGATTCTTATTCTAGAAATGAAGTTATCAGATGTATCCACATTGGCTTACTTTGTGTTCAGGAGGATCCGGCTGACAGACCTACAATGCAATCACTAGTTCTTATGCTCAACAGCTACTCTGTTAGTCTACCATTACCTCAAGAGCCAGCATTTGTCCATAGGACTAGATCGGAGTTCATGCCAAAGATACCGCTGGAGTCTGATCAGTCTATCAGCAAGTCAAGTCCGTCGGTTTATGAAGGATCTTTCACTGAAGTATATCCTCGATAA

Protein Analysis

657

Amino Acids

73.11

Weight (kDa)

6.26

Isoelectric Point (pI)

46.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stress-antifung PF01657 29 - 125 2.4e-28 Salt stress response/antifungal
Stress-antifung PF01657 147 - 236 8.7e-11 Salt stress response/antifungal
Pkinase PF00069 334 - 601 3e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 336 - 602 7.2e-49 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000283)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G45860 AT4G05200 AT4G05200 AT4G11521 AT4G11530 AT4G23130 AT4G23130 AT4G23130 AT4G23140 AT4G23140 AT4G23150 AT4G23160 AT4G23170 AT4G23180 AT4G23180 AT4G23180 AT4G23230 AT4G23270 AT4G23270 AT4G23270 AT4G23280 AT4G23280 AT4G23280 AT4G23310 AT4G23310
fragaria_vesca FvH4_3g02772 FvH4_3g02772 FvH4_3g02773 FvH4_3g02773 FvH4_3g02774 FvH4_3g02800 FvH4_3g02820 FvH4_3g02820 FvH4_3g02830
malus_domestica MD00G1101900.v1.1 MD00G1102100.v1.1 MD00G1102200.v1.1 MD01G1098200.v1.1 MD05G1340000.v1.1 MD05G1340300.v1.1 MD07G1014100.v1.1 MD07G1014300.v1.1 MD07G1014400.v1.1 MD07G1014700.v1.1 MD10G1312900.v1.1 MD11G1022600.v1.1 MD11G1291500.v1.1 MD14G1207100.v1.1 MD16G1096600.v1.1
prunus_persica Prupe.4G027300_v2.0.a1 Prupe.4G027400_v2.0.a1 Prupe.4G027700_v2.0.a1 Prupe.4G027800_v2.0.a1
pyrus_communis pycom05g30960 pycom10g00940 pycom10g00950 pycom10g26430 pycom10g26450 pycom10g26460 pycom10g26470 pycom10g26480 pycom10g26490 pycom10g26500 pycom10g26510 pycom10g26570 pycom11g01820 pycom11g04000 pycom14g17130 pycom14g17140 pycom15g28820
rosa_chinensis RchiOBHm_Chr4g0408141 RchiOBHm_Chr5g0004211 RchiOBHm_Chr5g0004231 RchiOBHm_Chr5g0004241 RchiOBHm_Chr5g0004261
rosa_laevigata RLG00000008668 RLG00000008672 RLG00000031212 RLG00000031217
rosa_multiflora Rmu_co8162658.1_g000001 Rmu_co8336297.1_g000001 Rmu_sc0002073.1_g000001 Rmu_sc0004964.1_g000002 Rmu_sc0004964.1_g000003 Rmu_sc0004964.1_g000007 Rmu_sc0006006.1_g000007 Rmu_sc0007868.1_g000015 Rmu_sc0007868.1_g000016 Rmu_sc0007868.1_g000017 Rmu_sc0007868.1_g000021 Rmu_sc0007868.1_g000023 Rmu_sc0019654.1_g000002 Rmu_sc0038540.1_g000001
rosa_roxburghii Rroxscaffold_1G00071450 Rroxscaffold_1G00071460 Rroxscaffold_1G00071470 Rroxscaffold_1G00071560 Rroxscaffold_1G00071720 Rroxscaffold_1G00071770 Rroxscaffold_5G00352640
rosa_rugosa Rorug04G0083700 Rorug04G0410700 Rorug04G0410800 Rorug04G0410900
rosa_samantha Rh4BG145400 Rh4CG154900 Rh4DG141900 Rh5BG037500 Rh5BG037600 Rh5BG037700 Rh5CG041000 Rh5CG041100 Rh5CG041200 Rh5CG041300 Rh5CG041400 Rh5DG037100
rosa_wichuraiana Rw4G012130 Rw5G003450 Rw5G003460 Rw5G003470 Rw5G003480 Rw5G003640 Rw5G003660 Rw5G003670 Rw5G003680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 720
AatII GACGTC 2 cut(s) 255, 731
Acc65I GGTACC 1 cut(s) 971
AccB1I GGYRCC 2 cut(s) 745, 971
AccB7I CCANNNNNTGG 1 cut(s) 466
AccI GTMKAC 3 cut(s) 225, 1041, 1831
AccII CGCG 3 cut(s) 174, 246, 524
AclWI GGATC 6 cut(s) 7, 1685, 1698, 1763, 1776, 1954
AcoI YGGCCR 3 cut(s) 489, 822, 1102
AcsI RAATTY 2 cut(s) 1118, 1682
AcyI GRCGYC 3 cut(s) 252, 728, 746
AfaI GTAC 6 cut(s) 344, 563, 905, 915, 973, 1137
AfeI AGCGCT 1 cut(s) 311
AfiI CCNNNNNNNGG 5 cut(s) 31, 270, 466, 749, 1165
AflIII ACRYGT 1 cut(s) 1503
AgeI ACCGGT 1 cut(s) 450
AgsI TTSAA 3 cut(s) 837, 980, 1154
AhlI ACTAGT 1 cut(s) 1798
AjnI CCWGG 4 cut(s) 39, 459, 748, 806
AjuI GAANNNNNNNTTGG 4 cut(s) 362, 394, 1014, 1046
AluBI AGCT 8 cut(s) 48, 84, 558, 567, 983, 1076, 1150, 1818
AluI AGCT 8 cut(s) 48, 84, 558, 567, 983, 1076, 1150, 1818
Alw21I GWGCWC 3 cut(s) 581, 779, 1114
Alw26I GTCTC 2 cut(s) 1285, 1512
Alw44I GTGCAC 2 cut(s) 577, 1110
AlwI GGATC 6 cut(s) 7, 1685, 1698, 1763, 1776, 1954
Ama87I CYCGRG 1 cut(s) 1320
Aor51HI AGCGCT 1 cut(s) 311
AoxI GGCC 5 cut(s) 202, 229, 489, 822, 1102
ApaLI GTGCAC 2 cut(s) 577, 1110
ApeKI GCWGC 7 cut(s) 84, 307, 312, 438, 648, 842, 983
ApoI RAATTY 2 cut(s) 1118, 1682
AsiGI ACCGGT 1 cut(s) 450
Asp718I GGTACC 1 cut(s) 971
AspLEI GCGC 2 cut(s) 312, 748
AspS9I GGNCC 2 cut(s) 273, 1633
AsuC2I CCSGG 4 cut(s) 317, 405, 642, 1351
AsuHPI GGTGA 4 cut(s) 28, 97, 247, 260
AsuII TTCGAA 1 cut(s) 503
AvaI CYCGRG 1 cut(s) 1320
AvaII GGWCC 2 cut(s) 273, 1633
BaeGI GKGCMC 2 cut(s) 581, 1114
BalI TGGCCA 3 cut(s) 491, 824, 1104
BamHI GGATCC 2 cut(s) 1690, 1768
BanI GGYRCC 2 cut(s) 745, 971
BanII GRGCYC 1 cut(s) 757
BarI GAAGNNNNNNTAC 4 cut(s) 1134, 1166, 1589, 1621
BbsI GAAGAC 3 cut(s) 94, 608, 1350
Bbv12I GWGCWC 3 cut(s) 581, 779, 1114
BbvI GCAGC 7 cut(s) 71, 299, 319, 450, 635, 829, 970
BccI CCATC 4 cut(s) 1055, 1126, 1394, 1661
BceAI ACGGC 3 cut(s) 244, 526, 815
BciT130I CCWGG 4 cut(s) 41, 461, 750, 808
BciVI GTATCC 2 cut(s) 690, 1748
BclI TGATCA 2 cut(s) 1456, 1906
BcnI CCSGG 4 cut(s) 317, 405, 642, 1351
BcoDI GTCTC 2 cut(s) 1285, 1512
BcuI ACTAGT 1 cut(s) 1798
BfaI CTAG 6 cut(s) 594, 942, 1577, 1716, 1799, 1869
BfmI CTRYAG 1 cut(s) 1555
BfoI RGCGCY 2 cut(s) 313, 749
BfuI GTATCC 2 cut(s) 690, 1748
BglI GCCNNNNNGGC 1 cut(s) 497
Bme1390I CCNGG 8 cut(s) 41, 317, 405, 461, 642, 750, 808, 1351
Bme18I GGWCC 2 cut(s) 273, 1633
BmeT110I CYCGRG 1 cut(s) 1320
BmgT120I GGNCC 2 cut(s) 273, 1633
BmiI GGNNCC 5 cut(s) 625, 747, 973, 1692, 1770
BmrFI CCNGG 8 cut(s) 41, 317, 405, 461, 642, 750, 808, 1351
BmsI GCATC 2 cut(s) 1197, 1420
BpiI GAAGAC 3 cut(s) 94, 608, 1350
BpmI CTGGAG 2 cut(s) 1494, 1919
Bpu10I CCTNAGC 1 cut(s) 568
Bpu14I TTCGAA 1 cut(s) 503
BpuEI CTTGAG 2 cut(s) 1449, 1827
BpuMI CCSGG 4 cut(s) 317, 405, 642, 1351
BsaBI GATNNNNATC 1 cut(s) 606
BsaHI GRCGYC 3 cut(s) 252, 728, 746
BsaJI CCNNGG 8 cut(s) 39, 244, 297, 404, 492, 749, 1105, 1321
BsaWI WCCGGW 1 cut(s) 450
BsaXI ACNNNNNCTCC 2 cut(s) 182, 212
Bsc4I CCNNNNNNNGG 5 cut(s) 31, 270, 466, 749, 1165
Bse118I RCCGGY 4 cut(s) 179, 270, 450, 742
Bse1I ACTGG 3 cut(s) 124, 1143, 1284
Bse3DI GCAATG 2 cut(s) 825, 1527
Bse8I GATNNNNATC 1 cut(s) 606
BseBI CCWGG 4 cut(s) 41, 461, 750, 808
BseDI CCNNGG 8 cut(s) 39, 244, 297, 404, 492, 749, 1105, 1321
BseGI GGATG 1 cut(s) 1411
BseJI GATNNNNATC 1 cut(s) 606
BseLI CCNNNNNNNGG 5 cut(s) 31, 270, 466, 749, 1165
BseMI GCAATG 2 cut(s) 825, 1527
BseMII CTCAG 3 cut(s) 387, 1254, 1691
BseNI ACTGG 3 cut(s) 124, 1143, 1284
BseRI GAGGAG 1 cut(s) 148
BseSI GKGCMC 2 cut(s) 581, 1114
BseXI GCAGC 7 cut(s) 71, 299, 319, 450, 635, 829, 970
BsgI GTGCAG 1 cut(s) 326
Bsh1236I CGCG 3 cut(s) 174, 246, 524
Bsh1285I CGRYCG 1 cut(s) 321
BshFI GGCC 5 cut(s) 204, 231, 491, 824, 1104
BshNI GGYRCC 2 cut(s) 745, 971
BshTI ACCGGT 1 cut(s) 450
BsiEI CGRYCG 1 cut(s) 321
BsiHKAI GWGCWC 3 cut(s) 581, 779, 1114
BsiHKCI CYCGRG 1 cut(s) 1320
BslFI GGGAC 2 cut(s) 681, 1211
BslI CCNNNNNNNGG 5 cut(s) 31, 270, 466, 749, 1165
BsmAI GTCTC 2 cut(s) 1285, 1512
BsmBI CGTCTC 1 cut(s) 1285
BsmFI GGGAC 2 cut(s) 681, 1211
BsmI GAATGC 1 cut(s) 1627
BsnI GGCC 5 cut(s) 204, 231, 491, 824, 1104
BsoBI CYCGRG 1 cut(s) 1320
Bsp119I TTCGAA 1 cut(s) 503
Bsp1286I GDGCHC 4 cut(s) 581, 757, 779, 1114
Bsp1407I TGTACA 1 cut(s) 561
Bsp143I GATC 9 cut(s) 12, 864, 1372, 1456, 1690, 1768, 1871, 1906, 1946
Bsp68I TCGCGA 1 cut(s) 524
BspANI GGCC 5 cut(s) 204, 231, 491, 824, 1104
BspCNI CTCAG 3 cut(s) 388, 1255, 1692
BspFNI CGCG 3 cut(s) 174, 246, 524
BspLI GGNNCC 5 cut(s) 625, 747, 973, 1692, 1770
BspPI GGATC 6 cut(s) 7, 1685, 1698, 1763, 1776, 1954
BspT104I TTCGAA 1 cut(s) 503
BspT107I GGYRCC 2 cut(s) 745, 971
BsrDI GCAATG 2 cut(s) 825, 1527
BsrFI RCCGGY 4 cut(s) 179, 270, 450, 742
BsrGI TGTACA 1 cut(s) 561
BsrI ACTGG 3 cut(s) 124, 1143, 1284
BssAI RCCGGY 4 cut(s) 179, 270, 450, 742
BssECI CCNNGG 8 cut(s) 39, 244, 297, 404, 492, 749, 1105, 1321
BssMI GATC 9 cut(s) 12, 864, 1372, 1456, 1690, 1768, 1871, 1906, 1946
BssNI GRCGYC 3 cut(s) 252, 728, 746
BssT1I CCWWGG 1 cut(s) 1105
Bst2UI CCWGG 4 cut(s) 41, 461, 750, 808
Bst4CI ACNGT 4 cut(s) 100, 223, 496, 781
Bst6I CTCTTC 3 cut(s) 26, 149, 239
BstACI GRCGYC 3 cut(s) 252, 728, 746
BstAUI TGTACA 1 cut(s) 561
BstBI TTCGAA 1 cut(s) 503
BstC8I GCNNGC 7 cut(s) 268, 356, 475, 581, 744, 1384, 1851
BstDEI CTNAG 7 cut(s) 396, 568, 885, 1263, 1355, 1570, 1700
BstDSI CCRYGG 2 cut(s) 244, 492
BstEII GGTNACC 1 cut(s) 34
BstF5I GGATG 1 cut(s) 1411
BstFNI CGCG 3 cut(s) 174, 246, 524
BstH2I RGCGCY 2 cut(s) 313, 749
BstHHI GCGC 2 cut(s) 312, 748
BstKTI GATC 9 cut(s) 15, 867, 1375, 1459, 1693, 1771, 1874, 1909, 1949
BstMAI GTCTC 2 cut(s) 1285, 1512
BstMBI GATC 9 cut(s) 12, 864, 1372, 1456, 1690, 1768, 1871, 1906, 1946
BstMCI CGRYCG 1 cut(s) 321
BstMWI GCNNNNNNNGC 9 cut(s) 180, 276, 360, 497, 564, 580, 745, 752, 1316
BstNI CCWGG 4 cut(s) 41, 461, 750, 808
BstNSI RCATGY 1 cut(s) 1507
BstPI GGTNACC 1 cut(s) 34
BstSCI CCNGG 8 cut(s) 39, 315, 403, 459, 640, 748, 806, 1349
BstSFI CTRYAG 1 cut(s) 1555
BstSLI GKGCMC 2 cut(s) 581, 1114
BstUI CGCG 3 cut(s) 174, 246, 524
BstV1I GCAGC 7 cut(s) 71, 299, 319, 450, 635, 829, 970
BstV2I GAAGAC 3 cut(s) 94, 608, 1350
BstX2I RGATCY 3 cut(s) 1690, 1768, 1946
BstYI RGATCY 3 cut(s) 1690, 1768, 1946
BsuI GTATCC 2 cut(s) 690, 1748
BsuRI GGCC 5 cut(s) 204, 231, 491, 824, 1104
BtgI CCRYGG 2 cut(s) 244, 492
BtsCI GGATG 1 cut(s) 1411
BtsI GCAGTG 1 cut(s) 581
BtsIMutI CAGTG 6 cut(s) 36, 393, 581, 786, 1594, 1953
BtuMI TCGCGA 1 cut(s) 524
Cac8I GCNNGC 7 cut(s) 268, 356, 475, 581, 744, 1384, 1851
CfoI GCGC 2 cut(s) 312, 748
Cfr10I RCCGGY 4 cut(s) 179, 270, 450, 742
Cfr13I GGNCC 2 cut(s) 273, 1633
Cfr42I CCGCGG 1 cut(s) 247
CsiI ACCWGGT 1 cut(s) 459
Csp6I GTAC 6 cut(s) 343, 562, 904, 914, 972, 1136
CspAI ACCGGT 1 cut(s) 450
CviAII CATG 8 cut(s) 415, 478, 1340, 1435, 1504, 1526, 1646, 1882
CviQI GTAC 6 cut(s) 343, 562, 904, 914, 972, 1136
DdeI CTNAG 7 cut(s) 396, 568, 885, 1263, 1355, 1570, 1700
DinI GGCGCC 1 cut(s) 747
DpnI GATC 9 cut(s) 14, 866, 1374, 1458, 1692, 1770, 1873, 1908, 1948
DpnII GATC 9 cut(s) 12, 864, 1372, 1456, 1690, 1768, 1871, 1906, 1946
EaeI YGGCCR 3 cut(s) 489, 822, 1102
Eam1104I CTCTTC 3 cut(s) 26, 149, 239
EarI CTCTTC 3 cut(s) 26, 149, 239
EciI GGCGGA 1 cut(s) 759
Eco130I CCWWGG 1 cut(s) 1105
Eco24I GRGCYC 1 cut(s) 757
Eco32I GATATC 1 cut(s) 1552
Eco47I GGWCC 2 cut(s) 273, 1633
Eco47III AGCGCT 1 cut(s) 311
Eco88I CYCGRG 1 cut(s) 1320
Eco91I GGTNACC 1 cut(s) 34
EcoO109I RGGNCCY 1 cut(s) 1633
EcoO65I GGTNACC 1 cut(s) 34
EcoRII CCWGG 4 cut(s) 39, 459, 748, 806
EcoRV GATATC 1 cut(s) 1552
EcoT14I CCWWGG 1 cut(s) 1105
EcoT22I ATGCAT 2 cut(s) 1413, 1527
EcoT38I GRGCYC 1 cut(s) 757
EgeI GGCGCC 1 cut(s) 747
EheI GGCGCC 1 cut(s) 747
ErhI CCWWGG 1 cut(s) 1105
Esp3I CGTCTC 1 cut(s) 1285
FaeI CATG 8 cut(s) 418, 481, 1343, 1438, 1507, 1529, 1649, 1885
FalI AAGNNNNNCTT 3 cut(s) 32, 365, 397
FaqI GGGAC 2 cut(s) 681, 1211
FatI CATG 8 cut(s) 414, 477, 1339, 1434, 1503, 1525, 1645, 1881
FauI CCCGC 2 cut(s) 647, 1303
FauNDI CATATG 1 cut(s) 1495
FbaI TGATCA 2 cut(s) 1456, 1906
FblI GTMKAC 3 cut(s) 225, 1041, 1831
FokI GGATG 1 cut(s) 1398
FriOI GRGCYC 1 cut(s) 757
FspBI CTAG 6 cut(s) 594, 942, 1577, 1716, 1799, 1869
GlaI GCGC 2 cut(s) 311, 747
GsuI CTGGAG 2 cut(s) 1494, 1919
HaeII RGCGCY 2 cut(s) 313, 749
HaeIII GGCC 5 cut(s) 204, 231, 491, 824, 1104
HhaI GCGC 2 cut(s) 312, 748
Hin1I GRCGYC 3 cut(s) 252, 728, 746
Hin1II CATG 8 cut(s) 418, 481, 1343, 1438, 1507, 1529, 1649, 1885
Hin6I GCGC 2 cut(s) 310, 746
HinP1I GCGC 2 cut(s) 310, 746
HindIII AAGCTT 2 cut(s) 46, 1148
HinfI GANTC 5 cut(s) 398, 950, 1346, 1706, 1901
HphI GGTGA 4 cut(s) 28, 97, 247, 260
Hpy166II GTNNAC 5 cut(s) 226, 579, 1042, 1112, 1832
Hpy8I GTNNAC 5 cut(s) 226, 579, 1042, 1112, 1832
Hpy99I CGWCG 7 cut(s) 58, 350, 392, 512, 529, 730, 1936
HpyAV CCTTC 7 cut(s) 26, 130, 388, 475, 1016, 1187, 1937
HpyCH4III ACNGT 4 cut(s) 100, 223, 496, 781
HpyCH4IV ACGT 4 cut(s) 252, 728, 1224, 1294
HpyF10VI GCNNNNNNNGC 9 cut(s) 180, 276, 360, 497, 564, 580, 745, 752, 1316
HpyF3I CTNAG 7 cut(s) 396, 568, 885, 1263, 1355, 1570, 1700
HpySE526I ACGT 4 cut(s) 252, 728, 1224, 1294
Hsp92I GRCGYC 3 cut(s) 252, 728, 746
Hsp92II CATG 8 cut(s) 418, 481, 1343, 1438, 1507, 1529, 1649, 1885
HspAI GCGC 2 cut(s) 310, 746
KasI GGCGCC 1 cut(s) 745
KpnI GGTACC 1 cut(s) 975
KroI GCCGGC 1 cut(s) 742
KroNI GCCGGC 1 cut(s) 744
Ksp22I TGATCA 2 cut(s) 1456, 1906
KspI CCGCGG 1 cut(s) 247
Kzo9I GATC 9 cut(s) 12, 864, 1372, 1456, 1690, 1768, 1871, 1906, 1946
LmnI GCTCC 2 cut(s) 363, 623
Lsp1109I GCAGC 7 cut(s) 71, 299, 319, 450, 635, 829, 970
LweI GCATC 2 cut(s) 1197, 1420
MabI ACCWGGT 1 cut(s) 459
MaeI CTAG 6 cut(s) 594, 942, 1577, 1716, 1799, 1869
MaeII ACGT 4 cut(s) 252, 728, 1224, 1294
MaeIII GTNAC 8 cut(s) 34, 248, 253, 280, 428, 781, 1295, 1604
MalI GATC 9 cut(s) 14, 866, 1374, 1458, 1692, 1770, 1873, 1908, 1948
MboI GATC 9 cut(s) 12, 864, 1372, 1456, 1690, 1768, 1871, 1906, 1946
MfeI CAATTG 1 cut(s) 837
MflI RGATCY 3 cut(s) 1690, 1768, 1946
MhlI GDGCHC 4 cut(s) 581, 757, 779, 1114
MlsI TGGCCA 3 cut(s) 491, 824, 1104
MluNI TGGCCA 3 cut(s) 491, 824, 1104
Mly113I GGCGCC 1 cut(s) 746
MlyI GAGTC 4 cut(s) 407, 959, 1340, 1910
MmeI TCCRAC 5 cut(s) 77, 153, 186, 393, 1468
Mox20I TGGCCA 3 cut(s) 491, 824, 1104
Mph1103I ATGCAT 2 cut(s) 1413, 1527
MreI CGCCGGCG 1 cut(s) 742
MroNI GCCGGC 1 cut(s) 742
MscI TGGCCA 3 cut(s) 491, 824, 1104
MseI TTAA 3 cut(s) 456, 1122, 1377
Msp20I TGGCCA 3 cut(s) 491, 824, 1104
MspA1I CMGCKG 2 cut(s) 246, 1897
MspR9I CCNGG 8 cut(s) 41, 317, 405, 461, 642, 750, 808, 1351
MunI CAATTG 1 cut(s) 837
Mva1269I GAATGC 1 cut(s) 1627
MvaI CCWGG 4 cut(s) 41, 461, 750, 808
MvnI CGCG 3 cut(s) 174, 246, 524
MwoI GCNNNNNNNGC 9 cut(s) 180, 276, 360, 497, 564, 580, 745, 752, 1316
NaeI GCCGGC 1 cut(s) 744
NarI GGCGCC 1 cut(s) 746
NciI CCSGG 4 cut(s) 317, 405, 642, 1351
NdeI CATATG 1 cut(s) 1495
NdeII GATC 9 cut(s) 12, 864, 1372, 1456, 1690, 1768, 1871, 1906, 1946
NgoMIV GCCGGC 1 cut(s) 742
NlaIII CATG 8 cut(s) 418, 481, 1343, 1438, 1507, 1529, 1649, 1885
NlaIV GGNNCC 5 cut(s) 625, 747, 973, 1692, 1770
NmeAIII GCCGAG 1 cut(s) 798
NmuCI GTSAC 5 cut(s) 34, 248, 253, 280, 781
NruI TCGCGA 1 cut(s) 524
NsiI ATGCAT 2 cut(s) 1413, 1527
NspI RCATGY 1 cut(s) 1507
NspV TTCGAA 1 cut(s) 503
PciI ACATGT 1 cut(s) 1503
PcsI WCGNNNNNNNCGW 3 cut(s) 171, 500, 533
PctI GAATGC 1 cut(s) 1627
PdiI GCCGGC 1 cut(s) 744
PfeI GAWTC 1 cut(s) 1706
PflMI CCANNNNNTGG 1 cut(s) 466
PfoI TCCNGGA 1 cut(s) 806
PinAI ACCGGT 1 cut(s) 450
PleI GAGTC 4 cut(s) 406, 958, 1340, 1909
PluTI GGCGCC 1 cut(s) 749
PpsI GAGTC 4 cut(s) 406, 958, 1340, 1909
PpuMI RGGWCCY 1 cut(s) 1633
PscI ACATGT 1 cut(s) 1503
PsiI TTATAA 1 cut(s) 720
Psp5II RGGWCCY 1 cut(s) 1633
Psp6I CCWGG 4 cut(s) 39, 459, 748, 806
PspEI GGTNACC 1 cut(s) 34
PspGI CCWGG 4 cut(s) 39, 459, 748, 806
PspN4I GGNNCC 5 cut(s) 625, 747, 973, 1692, 1770
PspPI GGNCC 2 cut(s) 273, 1633
PspPPI RGGWCCY 1 cut(s) 1633
PsuI RGATCY 3 cut(s) 1690, 1768, 1946
RruI TCGCGA 1 cut(s) 524
RsaI GTAC 6 cut(s) 344, 563, 905, 915, 973, 1137
RsaNI GTAC 6 cut(s) 343, 562, 904, 914, 972, 1136
SacII CCGCGG 1 cut(s) 247
SaqAI TTAA 3 cut(s) 456, 1122, 1377
Sau3AI GATC 9 cut(s) 12, 864, 1372, 1456, 1690, 1768, 1871, 1906, 1946
Sau96I GGNCC 2 cut(s) 273, 1633
SchI GAGTC 4 cut(s) 407, 959, 1340, 1910
ScrFI CCNGG 8 cut(s) 41, 317, 405, 461, 642, 750, 808, 1351
SduI GDGCHC 4 cut(s) 581, 757, 779, 1114
SexAI ACCWGGT 1 cut(s) 459
SfaNI GCATC 2 cut(s) 1197, 1420
SfcI CTRYAG 1 cut(s) 1555
SfoI GGCGCC 1 cut(s) 747
Sfr303I CCGCGG 1 cut(s) 247
SfuI TTCGAA 1 cut(s) 503
SgrAI CRCCGGYG 1 cut(s) 742
SgrBI CCGCGG 1 cut(s) 247
SinI GGWCC 2 cut(s) 273, 1633
SmlI CTYRAG 2 cut(s) 1464, 1842
SmoI CTYRAG 2 cut(s) 1464, 1842
SpeI ACTAGT 1 cut(s) 1798
SspDI GGCGCC 1 cut(s) 745
SspMI CTAG 6 cut(s) 594, 942, 1577, 1716, 1799, 1869
StyD4I CCNGG 8 cut(s) 39, 315, 403, 459, 640, 748, 806, 1349
StyI CCWWGG 1 cut(s) 1105
TaaI ACNGT 4 cut(s) 100, 223, 496, 781
TaiI ACGT 4 cut(s) 255, 731, 1227, 1297
TaqI TCGA 7 cut(s) 321, 503, 725, 757, 1242, 1289, 1969
TatI WGTACW 2 cut(s) 561, 903
TauI GCSGC 5 cut(s) 246, 266, 473, 654, 742
TfiI GAWTC 1 cut(s) 1706
Tru1I TTAA 3 cut(s) 456, 1122, 1377
Tru9I TTAA 3 cut(s) 456, 1122, 1377
TscAI CASTG 6 cut(s) 36, 400, 581, 786, 1594, 1960
TseFI GTSAC 5 cut(s) 34, 248, 253, 280, 781
TseI GCWGC 7 cut(s) 84, 307, 312, 438, 648, 842, 983
Tsp45I GTSAC 5 cut(s) 34, 248, 253, 280, 781
TspDTI ATGAA 9 cut(s) 231, 403, 494, 717, 1233, 1356, 1737, 1870, 1956
TspGWI ACGGA 4 cut(s) 376, 560, 1683, 1920
TspRI CASTG 6 cut(s) 36, 400, 581, 786, 1594, 1960
Van91I CCANNNNNTGG 1 cut(s) 466
VneI GTGCAC 2 cut(s) 577, 1110
VpaK11BI GGWCC 2 cut(s) 273, 1633
XapI RAATTY 2 cut(s) 1118, 1682
XbaI TCTAGA 2 cut(s) 1576, 1715
XceI RCATGY 1 cut(s) 1507
XmiI GTMKAC 3 cut(s) 225, 1041, 1831
XspI CTAG 6 cut(s) 594, 942, 1577, 1716, 1799, 1869
ZraI GACGTC 2 cut(s) 253, 729
Zsp2I ATGCAT 2 cut(s) 1413, 1527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.