Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0000283

Physical Location & Seq

Genomic Coordinates Reverse (-)

39758570 .. 39761786

Gene Structure 3217 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1312900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2064 bp

ATGAAAAACATGGTTACCCTTCTTTTGATTTTCTCCGTTTTTACTCTGCTCAGCTTTCCGACCAGTACGGATGCTGATTACCTGGCTCACATCTGCCCAAACACCACCACCTTCACACCAAACTCCACCTTCCAGTCCAACCTCAACCGCCTCCTCTCCACCCTCTCCTCCAACGCTAACCGTTCCACCGGCTTCTACAACGCCACAGTCCAAAGCACAAACAACGCCGTCTACGGCCTTTTCCTCTGCCGCGGCGACGTCGTGGGCACCGACGCTTGCCAAACCTGTGTCACCACCGCAACCGCCGAGGCCCCAAAACGCTGCTCCGTAGAAAAACAGGTGGTGATCTGGTACGACGACTGCTTGCTACGCTACTCCAACGAGTCATTCTTCTCCACCGCCGCCGAGTCACCCCGCCTCTACATGTGGAACACGGAGAACGCCACCGACCCGACTCGGTTCAACCAGGTCCTGGGGGCCAGTATGAACCAGGTTGCCGGTCAGGCCGCCGGAGAAGCGGACAAGTTCGCGACGAATCAAGCAAATGTTAGCGGGTTGATTTCGGTGTACAGCCTCGGGCAGTGCACGCAGGACCTGTCGGAAGCGGATTGCAACCGCTGTTTACGGGGGACCATAGCCCTACTTCCGAGTTGCTGTAATGGGAAACTCGGAGGACGAGTTTTGTTTCCGAGTTGTAATGTTAGGTACGAAGTCTACCCCTTCTACGCCCAGAACGCTACGTTAGCACCTGAGCCTGCACCAGGGCCGCCTCCTCCTTCCCTCCTCCGCCTAAAGGTAAAGGGAATATGGTGTTGTGCTGTGGCTGCTGATTTTACCCATTTCTCAATATTTTTTGTTTATTTTGCAGGAAAAAGTAAAATCCCAACTAGGACAATTGTTGCCATTGTTGTACCAATATCTGTATCTGTGCTACTTTTTGTTGTGGGTTACTGCTGCATAACTAGGAGAGCAAGAAAGAAGTACAATCAAGCAGCAGCAGACGAACCAAGCGGTGAGAATGACATTTCTACTGTCGAGTCTTTGCAATTTGATTTTGCTACCATCCAAGCAGCTACGAGCAATTTCTCCGATGATCACAAGTTAGGCGAAGGTGGTTTCGGTCAAGTTTACAAGGGTACACTTTCAAACGGTCAAGAAGTAGCTGTGAAGAGGCTATCAAAGAACTCTGGCCAAGGCACAGAAGAGTTTAAGAACGAGATGGTATTGGTAGCCAAGCTTCAACACCGAAATTTGGTTAGGCTATTGGGATTTTGCTTGGAAGGCGAAGAAAAGATTCTTGTTTATGAATATGTGCTCAACAAAAGCCTCGACTGTTTCCTATTTGACCCTGAGAAACAGGGGCAATTGGATTGGTCAAGACGTTACAAGATTATTTCAGGAATTGCTCGAGGAATCATGTACCTGCATGAAGATTCTCAACTTAGAATTATACATCGTGATCTCAAAGCCAGCAATATATTGTTAGATGGGGACATGCATCCAAAAATATCTGATTTTGGCATGGCCAGGATCTTTGGGGTTGATCAAACTCAAGCAAACACAAATAGAATTGTCGGAACATATGGTTATATGTCTCCTGAGTATGCAATGCACGGGCATTTTTCTGTCAAGTCCGATCTGTATAGTTTCGGTGTTTTAGTGCTAGAAATCATCAGTGGCAAGAAGAACAGTAACTTCTTTCAGACCGATGCAGCTGAGGACCTCGCGAGCCATGCTTGGAAGTTATGGAGAGACGGGAAATCTTTGGAATTGTTGGATCAGTGTATGAGAGACTCTTATTCGAGGACTGAAGTAATCAGATGCATCCACATCGGCTTACTTTGTGTTCAGGAAGATCCAGCTGACAGGCCTACAATGCAATCAGTAGTTCTCATGCTCAATAGCTACTCTGTCAGTCTGGCATTACCTAAACAACCGGCATTTTTTCTCCAAAGCAGAGCAGTGGGGAACATGCCAAAGATAACGCTGGAGTCCGATCAATCTATCAGCAAGTCATCTCCGTCCGTTTATGAAGGATCTATCACTGAAGTATACCCTAGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0042742 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050896 GO:0051704 GO:0051707 GO:0071704 GO:0071944 GO:0098542 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr Stress-antifung

Protein Analysis

688

Amino Acids

76.02

Weight (kDa)

6.61

Isoelectric Point (pI)

39.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Stress-antifung	PF01657	32 - 128	2.4e-26	Salt stress response/antifungal
Stress-antifung	PF01657	146 - 238	9.5e-12	Salt stress response/antifungal
Pkinase	PF00069	363 - 630	3e-46	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	365 - 631	5.8e-50	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000283)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G45860 AT4G05200 AT4G05200 AT4G11521 AT4G11530 AT4G23130 AT4G23130 AT4G23130 AT4G23140 AT4G23140 AT4G23150 AT4G23160 AT4G23170 AT4G23180 AT4G23180 AT4G23180 AT4G23230 AT4G23270 AT4G23270 AT4G23270 AT4G23280 AT4G23280 AT4G23280 AT4G23310 AT4G23310
fragaria_vesca	FvH4_3g02772 FvH4_3g02772 FvH4_3g02773 FvH4_3g02773 FvH4_3g02774 FvH4_3g02800 FvH4_3g02820 FvH4_3g02820 FvH4_3g02830
malus_domestica	MD00G1101900.v1.1 MD00G1102100.v1.1 MD00G1102200.v1.1 MD01G1098200.v1.1 MD05G1340000.v1.1 MD05G1340300.v1.1 MD07G1014100.v1.1 MD07G1014300.v1.1 MD07G1014400.v1.1 MD07G1014700.v1.1 MD10G1312900.v1.1 MD11G1022600.v1.1 MD11G1291500.v1.1 MD14G1207100.v1.1 MD16G1096600.v1.1
prunus_persica	Prupe.4G027300_v2.0.a1 Prupe.4G027400_v2.0.a1 Prupe.4G027700_v2.0.a1 Prupe.4G027800_v2.0.a1
pyrus_communis	pycom05g30960 pycom10g00940 pycom10g00950 pycom10g26430 pycom10g26450 pycom10g26460 pycom10g26470 pycom10g26480 pycom10g26490 pycom10g26500 pycom10g26510 pycom10g26570 pycom11g01820 pycom11g04000 pycom14g17130 pycom14g17140 pycom15g28820
rosa_chinensis	RchiOBHm_Chr4g0408141 RchiOBHm_Chr5g0004211 RchiOBHm_Chr5g0004231 RchiOBHm_Chr5g0004241 RchiOBHm_Chr5g0004261
rosa_laevigata	RLG00000008668 RLG00000008672 RLG00000031212 RLG00000031217
rosa_multiflora	Rmu_co8162658.1_g000001 Rmu_co8336297.1_g000001 Rmu_sc0002073.1_g000001 Rmu_sc0004964.1_g000002 Rmu_sc0004964.1_g000003 Rmu_sc0004964.1_g000007 Rmu_sc0006006.1_g000007 Rmu_sc0007868.1_g000015 Rmu_sc0007868.1_g000016 Rmu_sc0007868.1_g000017 Rmu_sc0007868.1_g000021 Rmu_sc0007868.1_g000023 Rmu_sc0019654.1_g000002 Rmu_sc0038540.1_g000001
rosa_roxburghii	Rroxscaffold_1G00071450 Rroxscaffold_1G00071460 Rroxscaffold_1G00071470 Rroxscaffold_1G00071560 Rroxscaffold_1G00071720 Rroxscaffold_1G00071770 Rroxscaffold_5G00352640
rosa_rugosa	Rorug04G0083700 Rorug04G0410700 Rorug04G0410800 Rorug04G0410900
rosa_samantha	Rh4BG145400 Rh4CG154900 Rh4DG141900 Rh5BG037500 Rh5BG037600 Rh5BG037700 Rh5CG041000 Rh5CG041100 Rh5CG041200 Rh5CG041300 Rh5CG041400 Rh5DG037100
rosa_wichuraiana	Rw4G012130 Rw5G003450 Rw5G003460 Rw5G003470 Rw5G003480 Rw5G003640 Rw5G003660 Rw5G003670 Rw5G003680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	261
Acc36I	ACCTGC	1 cut(s)	1431
AccB1I	GGYRCC	1 cut(s)	266
AccB7I	CCANNNNNTGG	1 cut(s)	472
AccI	GTMKAC	3 cut(s)	231, 714, 2052
AccII	CGCG	3 cut(s)	252, 530, 1727
AclWI	GGATC	4 cut(s)	1538, 1785, 1850, 2044
AcoI	YGGCCR	2 cut(s)	1189, 1524
AcsI	RAATTY	1 cut(s)	1249
AcuI	CTGAAG	1 cut(s)	1830
AcyI	GRCGYC	1 cut(s)	258
AfaI	GTAC	8 cut(s)	67, 353, 569, 707, 912, 983, 1138, 1421
AfiI	CCNNNNNNNGG	4 cut(s)	472, 761, 793, 1252
AflIII	ACRYGT	1 cut(s)	423
AgsI	TTSAA	3 cut(s)	463, 1146, 1241
AjnI	CCWGG	6 cut(s)	81, 465, 471, 489, 760, 1526
AjuI	GAANNNNNNNTTGG	2 cut(s)	371, 403
AluBI	AGCT	7 cut(s)	54, 1073, 1163, 1237, 1715, 1862, 1905
AluI	AGCT	7 cut(s)	54, 1073, 1163, 1237, 1715, 1862, 1905
Alw21I	GWGCWC	2 cut(s)	587, 1317
Alw26I	GTCTC	3 cut(s)	1599, 1746, 1785
Alw44I	GTGCAC	1 cut(s)	583
AlwI	GGATC	4 cut(s)	1538, 1785, 1850, 2044
AlwNI	CAGNNNCTG	2 cut(s)	472, 595
Ama87I	CYCGRG	2 cut(s)	575, 1407
AoxI	GGCC	8 cut(s)	235, 309, 477, 504, 764, 1189, 1524, 1868
ApaLI	GTGCAC	1 cut(s)	583
ApeKI	GCWGC	7 cut(s)	321, 824, 954, 992, 995, 1070, 1712
ApoI	RAATTY	1 cut(s)	1249
Asp700I	GAANNNNTTC	1 cut(s)	1293
AspS9I	GGNCC	7 cut(s)	310, 469, 477, 592, 630, 764, 1720
AsuHPI	GGTGA	4 cut(s)	283, 355, 402, 1025
AvaI	CYCGRG	2 cut(s)	575, 1407
AvaII	GGWCC	4 cut(s)	469, 592, 630, 1720
BaeGI	GKGCMC	2 cut(s)	269, 587
BalI	TGGCCA	2 cut(s)	1191, 1526
BanI	GGYRCC	1 cut(s)	266
BarI	GAAGNNNNNNTAC	2 cut(s)	1221, 1253
Bbv12I	GWGCWC	2 cut(s)	587, 1317
BbvCI	CCTCAGC	1 cut(s)	1716
BbvI	GCAGC	7 cut(s)	308, 811, 941, 1004, 1007, 1082, 1724
BccI	CCATC	3 cut(s)	1070, 1213, 1481
BceAI	ACGGC	2 cut(s)	212, 250
BcgI	CGANNNNNNTGC	2 cut(s)	1813, 1847
BciT130I	CCWGG	6 cut(s)	83, 467, 473, 491, 762, 1528
BclI	TGATCA	2 cut(s)	1093, 1543
BcoDI	GTCTC	3 cut(s)	1599, 1746, 1785
BfaI	CTAG	4 cut(s)	888, 963, 1664, 2058
BfuAI	ACCTGC	1 cut(s)	1431
BglI	GCCNNNNNGGC	1 cut(s)	503
BlpI	GCTNAGC	1 cut(s)	50
Bme1390I	CCNGG	6 cut(s)	83, 467, 473, 491, 762, 1528
Bme18I	GGWCC	4 cut(s)	469, 592, 630, 1720
BmeT110I	CYCGRG	2 cut(s)	575, 1407
BmgT120I	GGNCC	7 cut(s)	310, 469, 477, 592, 630, 764, 1720
BmiI	GGNNCC	4 cut(s)	268, 312, 478, 631
BmrFI	CCNGG	6 cut(s)	83, 467, 473, 491, 762, 1528
BmsI	GCATC	5 cut(s)	61, 1507, 1699, 1811, 1833
BpmI	CTGGAG	1 cut(s)	2009
Bpu10I	CCTNAGC	2 cut(s)	750, 1716
Bpu1102I	GCTNAGC	1 cut(s)	50
BpuEI	CTTGAG	1 cut(s)	1536
BsaHI	GRCGYC	1 cut(s)	258
BsaJI	CCNNGG	6 cut(s)	250, 306, 472, 574, 761, 1192
BsaXI	ACNNNNNCTCC	2 cut(s)	663, 693
Bsc4I	CCNNNNNNNGG	4 cut(s)	472, 761, 793, 1252
Bse118I	RCCGGY	3 cut(s)	188, 497, 1936
Bse1I	ACTGG	3 cut(s)	63, 133, 480
Bse3DI	GCAATG	1 cut(s)	1614
BseBI	CCWGG	6 cut(s)	83, 467, 473, 491, 762, 1528
BseDI	CCNNGG	6 cut(s)	250, 306, 472, 574, 761, 1192
BseGI	GGATG	4 cut(s)	76, 1062, 1498, 1824
BseLI	CCNNNNNNNGG	4 cut(s)	472, 761, 793, 1252
BseMI	GCAATG	1 cut(s)	1614
BseMII	CTCAG	5 cut(s)	64, 741, 1341, 1590, 1707
BseNI	ACTGG	3 cut(s)	63, 133, 480
BseRI	GAGGAG	4 cut(s)	143, 157, 762, 773
BseSI	GKGCMC	2 cut(s)	269, 587
BseXI	GCAGC	7 cut(s)	308, 811, 941, 1004, 1007, 1082, 1724
BsgI	GTGCAG	1 cut(s)	741
Bsh1236I	CGCG	3 cut(s)	252, 530, 1727
BshFI	GGCC	8 cut(s)	237, 311, 479, 506, 766, 1191, 1526, 1870
BshNI	GGYRCC	1 cut(s)	266
BsiHKAI	GWGCWC	2 cut(s)	587, 1317
BsiHKCI	CYCGRG	2 cut(s)	575, 1407
BsiSI	CCGG	4 cut(s)	189, 498, 510, 1937
BslFI	GGGAC	2 cut(s)	643, 1505
BslI	CCNNNNNNNGG	4 cut(s)	472, 761, 793, 1252
BsmAI	GTCTC	3 cut(s)	1599, 1746, 1785
BsmBI	CGTCTC	1 cut(s)	1746
BsmFI	GGGAC	2 cut(s)	643, 1505
BsnI	GGCC	8 cut(s)	237, 311, 479, 506, 766, 1191, 1526, 1870
BsoBI	CYCGRG	2 cut(s)	575, 1407
Bsp1286I	GDGCHC	3 cut(s)	269, 587, 1317
Bsp1407I	TGTACA	1 cut(s)	567
Bsp1720I	GCTNAGC	1 cut(s)	50
Bsp68I	TCGCGA	2 cut(s)	530, 1727
BspANI	GGCC	8 cut(s)	237, 311, 479, 506, 766, 1191, 1526, 1870
BspCNI	CTCAG	5 cut(s)	63, 742, 1342, 1591, 1708
BspFNI	CGCG	3 cut(s)	252, 530, 1727
BspLI	GGNNCC	4 cut(s)	268, 312, 478, 631
BspMI	ACCTGC	1 cut(s)	1431
BspPI	GGATC	4 cut(s)	1538, 1785, 1850, 2044
BspT107I	GGYRCC	1 cut(s)	266
BsrDI	GCAATG	1 cut(s)	1614
BsrFI	RCCGGY	3 cut(s)	188, 497, 1936
BsrGI	TGTACA	1 cut(s)	567
BsrI	ACTGG	3 cut(s)	63, 133, 480
BssAI	RCCGGY	3 cut(s)	188, 497, 1936
BssECI	CCNNGG	6 cut(s)	250, 306, 472, 574, 761, 1192
BssNAI	GTATAC	1 cut(s)	2053
BssNI	GRCGYC	1 cut(s)	258
BssT1I	CCWWGG	1 cut(s)	1192
Bst1107I	GTATAC	1 cut(s)	2053
Bst2UI	CCWGG	6 cut(s)	83, 467, 473, 491, 762, 1528
Bst4CI	ACNGT	6 cut(s)	182, 208, 1033, 1151, 1334, 1691
Bst6I	CTCTTC	2 cut(s)	1163, 1197
BstACI	GRCGYC	1 cut(s)	258
BstAUI	TGTACA	1 cut(s)	567
BstC8I	GCNNGC	6 cut(s)	277, 365, 587, 756, 1471, 1729
BstDEI	CTNAG	6 cut(s)	50, 750, 1350, 1442, 1599, 1716
BstDSI	CCRYGG	1 cut(s)	250
BstEII	GGTNACC	1 cut(s)	13
BstENI	CCTNNNNNAGG	1 cut(s)	759
BstF5I	GGATG	4 cut(s)	76, 1062, 1498, 1824
BstFNI	CGCG	3 cut(s)	252, 530, 1727
BstMAI	GTCTC	3 cut(s)	1599, 1746, 1785
BstNI	CCWGG	6 cut(s)	83, 467, 473, 491, 762, 1528
BstNSI	RCATGY	3 cut(s)	427, 1498, 1975
BstPI	GGTNACC	1 cut(s)	13
BstSCI	CCNGG	6 cut(s)	81, 465, 471, 489, 760, 1526
BstSLI	GKGCMC	2 cut(s)	269, 587
BstUI	CGCG	3 cut(s)	252, 530, 1727
BstV1I	GCAGC	7 cut(s)	308, 811, 941, 1004, 1007, 1082, 1724
BstX2I	RGATCY	3 cut(s)	1530, 1855, 2036
BstYI	RGATCY	3 cut(s)	1530, 1855, 2036
BstZ17I	GTATAC	1 cut(s)	2053
BsuRI	GGCC	8 cut(s)	237, 311, 479, 506, 766, 1191, 1526, 1870
BtgI	CCRYGG	1 cut(s)	250
BtsCI	GGATG	4 cut(s)	76, 1062, 1498, 1824
BtsI	GCAGTG	2 cut(s)	587, 1968
BtsIMutI	CAGTG	5 cut(s)	587, 1681, 1787, 1968, 2043
BtuMI	TCGCGA	2 cut(s)	530, 1727
BveI	ACCTGC	1 cut(s)	1431
Cac8I	GCNNGC	6 cut(s)	277, 365, 587, 756, 1471, 1729
CaiI	CAGNNNCTG	2 cut(s)	472, 595
Cfr10I	RCCGGY	3 cut(s)	188, 497, 1936
Cfr13I	GGNCC	7 cut(s)	310, 469, 477, 592, 630, 764, 1720
Cfr42I	CCGCGG	1 cut(s)	253
CseI	GACGC	1 cut(s)	281
CsiI	ACCWGGT	2 cut(s)	465, 489
Csp6I	GTAC	8 cut(s)	66, 352, 568, 706, 911, 982, 1137, 1420
CviAII	CATG	9 cut(s)	10, 424, 1417, 1427, 1495, 1522, 1733, 1894, 1972
CviQI	GTAC	8 cut(s)	66, 352, 568, 706, 911, 982, 1137, 1420
DdeI	CTNAG	6 cut(s)	50, 750, 1350, 1442, 1599, 1716
EaeI	YGGCCR	2 cut(s)	1189, 1524
Eam1104I	CTCTTC	2 cut(s)	1163, 1197
EarI	CTCTTC	2 cut(s)	1163, 1197
EciI	GGCGGA	1 cut(s)	776
Eco130I	CCWWGG	1 cut(s)	1192
Eco147I	AGGCCT	1 cut(s)	1870
Eco47I	GGWCC	4 cut(s)	469, 592, 630, 1720
Eco57I	CTGAAG	1 cut(s)	1830
Eco88I	CYCGRG	2 cut(s)	575, 1407
Eco91I	GGTNACC	1 cut(s)	13
EcoNI	CCTNNNNNAGG	1 cut(s)	759
EcoO109I	RGGNCCY	4 cut(s)	310, 469, 592, 1720
EcoO65I	GGTNACC	1 cut(s)	13
EcoRII	CCWGG	6 cut(s)	81, 465, 471, 489, 760, 1526
EcoT14I	CCWWGG	1 cut(s)	1192
EcoT22I	ATGCAT	2 cut(s)	1500, 1826
ErhI	CCWWGG	1 cut(s)	1192
Esp3I	CGTCTC	1 cut(s)	1746
FaeI	CATG	9 cut(s)	13, 427, 1420, 1430, 1498, 1525, 1736, 1897, 1975
FaqI	GGGAC	2 cut(s)	643, 1505
FatI	CATG	9 cut(s)	9, 423, 1416, 1426, 1494, 1521, 1732, 1893, 1971
FauI	CCCGC	2 cut(s)	422, 545
FauNDI	CATATG	1 cut(s)	1582
FbaI	TGATCA	2 cut(s)	1093, 1543
FblI	GTMKAC	3 cut(s)	231, 714, 2052
FokI	GGATG	4 cut(s)	83, 1049, 1485, 1811
FspBI	CTAG	4 cut(s)	888, 963, 1664, 2058
GsuI	CTGGAG	1 cut(s)	2009
HaeIII	GGCC	8 cut(s)	237, 311, 479, 506, 766, 1191, 1526, 1870
HapII	CCGG	4 cut(s)	189, 498, 510, 1937
HgaI	GACGC	1 cut(s)	281
Hin1I	GRCGYC	1 cut(s)	258
Hin1II	CATG	9 cut(s)	13, 427, 1420, 1430, 1498, 1525, 1736, 1897, 1975
HindIII	AAGCTT	1 cut(s)	1235
HpaII	CCGG	4 cut(s)	189, 498, 510, 1937
HphI	GGTGA	4 cut(s)	283, 355, 402, 1025
Hpy166II	GTNNAC	8 cut(s)	232, 568, 585, 623, 715, 1129, 1139, 2053
Hpy188III	TCNNGA	8 cut(s)	529, 1154, 1377, 1398, 1457, 1598, 1726, 1850
Hpy8I	GTNNAC	8 cut(s)	232, 568, 585, 623, 715, 1129, 1139, 2053
Hpy99I	CGWCG	5 cut(s)	260, 263, 275, 359, 535
HpyAV	CCTTC	8 cut(s)	29, 121, 139, 730, 786, 1103, 1274, 2027
HpyCH4III	ACNGT	6 cut(s)	182, 208, 1033, 1151, 1334, 1691
HpyCH4IV	ACGT	3 cut(s)	258, 740, 1381
HpyF3I	CTNAG	6 cut(s)	50, 750, 1350, 1442, 1599, 1716
HpySE526I	ACGT	3 cut(s)	258, 740, 1381
Hsp92I	GRCGYC	1 cut(s)	258
Hsp92II	CATG	9 cut(s)	13, 427, 1420, 1430, 1498, 1525, 1736, 1897, 1975
Ksp22I	TGATCA	2 cut(s)	1093, 1543
KspI	CCGCGG	1 cut(s)	253
LmnI	GCTCC	1 cut(s)	329
Lsp1109I	GCAGC	7 cut(s)	308, 811, 941, 1004, 1007, 1082, 1724
LweI	GCATC	5 cut(s)	61, 1507, 1699, 1811, 1833
MabI	ACCWGGT	2 cut(s)	465, 489
MaeI	CTAG	4 cut(s)	888, 963, 1664, 2058
MaeII	ACGT	3 cut(s)	258, 740, 1381
MaeIII	GTNAC	6 cut(s)	13, 289, 408, 947, 1382, 1691
MboII	GAAGA	7 cut(s)	382, 1180, 1214, 1298, 1442, 1696, 1865
MfeI	CAATTG	2 cut(s)	894, 1364
MflI	RGATCY	3 cut(s)	1530, 1855, 2036
MhlI	GDGCHC	3 cut(s)	269, 587, 1317
MlsI	TGGCCA	2 cut(s)	1191, 1526
MluCI	AATT	9 cut(s)	894, 1046, 1081, 1249, 1364, 1401, 1446, 1569, 1769
MluNI	TGGCCA	2 cut(s)	1191, 1526
MlyI	GAGTC	6 cut(s)	392, 416, 448, 1046, 1787, 2000
MmeI	TCCRAC	7 cut(s)	83, 162, 195, 402, 579, 1555, 1755
Mox20I	TGGCCA	2 cut(s)	1191, 1526
Mph1103I	ATGCAT	2 cut(s)	1500, 1826
MroXI	GAANNNNTTC	1 cut(s)	1293
MscI	TGGCCA	2 cut(s)	1191, 1526
MseI	TTAA	1 cut(s)	1209
Msp20I	TGGCCA	2 cut(s)	1191, 1526
MspA1I	CMGCKG	4 cut(s)	252, 618, 1715, 1862
MspI	CCGG	4 cut(s)	189, 498, 510, 1937
MspR9I	CCNGG	6 cut(s)	83, 467, 473, 491, 762, 1528
MunI	CAATTG	2 cut(s)	894, 1364
MvaI	CCWGG	6 cut(s)	83, 467, 473, 491, 762, 1528
MvnI	CGCG	3 cut(s)	252, 530, 1727
NdeI	CATATG	1 cut(s)	1582
NlaIII	CATG	9 cut(s)	13, 427, 1420, 1430, 1498, 1525, 1736, 1897, 1975
NlaIV	GGNNCC	4 cut(s)	268, 312, 478, 631
NmeAIII	GCCGAG	2 cut(s)	331, 430
NmuCI	GTSAC	2 cut(s)	289, 408
NruI	TCGCGA	2 cut(s)	530, 1727
NsiI	ATGCAT	2 cut(s)	1500, 1826
NspI	RCATGY	3 cut(s)	427, 1498, 1975
PaeR7I	CTCGAG	1 cut(s)	1407
PceI	AGGCCT	1 cut(s)	1870
PciI	ACATGT	1 cut(s)	423
PcsI	WCGNNNNNNNCGW	1 cut(s)	267
PdmI	GAANNNNTTC	1 cut(s)	1293
PfeI	GAWTC	4 cut(s)	535, 1294, 1413, 1433
PflMI	CCANNNNNTGG	1 cut(s)	472
PleI	GAGTC	6 cut(s)	391, 415, 448, 1045, 1787, 1999
PpsI	GAGTC	6 cut(s)	391, 415, 448, 1045, 1787, 1999
PpuMI	RGGWCCY	3 cut(s)	469, 592, 1720
PscI	ACATGT	1 cut(s)	423
Psp5II	RGGWCCY	3 cut(s)	469, 592, 1720
Psp6I	CCWGG	6 cut(s)	81, 465, 471, 489, 760, 1526
PspEI	GGTNACC	1 cut(s)	13
PspGI	CCWGG	6 cut(s)	81, 465, 471, 489, 760, 1526
PspN4I	GGNNCC	4 cut(s)	268, 312, 478, 631
PspPI	GGNCC	7 cut(s)	310, 469, 477, 592, 630, 764, 1720
PspPPI	RGGWCCY	3 cut(s)	469, 592, 1720
PspXI	VCTCGAGB	1 cut(s)	1407
PstNI	CAGNNNCTG	2 cut(s)	472, 595
PsuI	RGATCY	3 cut(s)	1530, 1855, 2036
PvuII	CAGCTG	2 cut(s)	1715, 1862
RruI	TCGCGA	2 cut(s)	530, 1727
RsaI	GTAC	8 cut(s)	67, 353, 569, 707, 912, 983, 1138, 1421
RsaNI	GTAC	8 cut(s)	66, 352, 568, 706, 911, 982, 1137, 1420
SacII	CCGCGG	1 cut(s)	253
SaqAI	TTAA	1 cut(s)	1209
Sau96I	GGNCC	7 cut(s)	310, 469, 477, 592, 630, 764, 1720
SchI	GAGTC	6 cut(s)	392, 416, 448, 1046, 1787, 2000
ScrFI	CCNGG	6 cut(s)	83, 467, 473, 491, 762, 1528
SduI	GDGCHC	3 cut(s)	269, 587, 1317
SexAI	ACCWGGT	2 cut(s)	465, 489
SfaNI	GCATC	5 cut(s)	61, 1507, 1699, 1811, 1833
Sfr274I	CTCGAG	1 cut(s)	1407
Sfr303I	CCGCGG	1 cut(s)	253
SgrBI	CCGCGG	1 cut(s)	253
SinI	GGWCC	4 cut(s)	469, 592, 630, 1720
SlaI	CTCGAG	1 cut(s)	1407
SmlI	CTYRAG	2 cut(s)	1407, 1551
SmoI	CTYRAG	2 cut(s)	1407, 1551
Sse9I	AATT	9 cut(s)	894, 1046, 1081, 1249, 1364, 1401, 1446, 1569, 1769
SseBI	AGGCCT	1 cut(s)	1870
SspI	AATATT	1 cut(s)	849
SspMI	CTAG	4 cut(s)	888, 963, 1664, 2058
StuI	AGGCCT	1 cut(s)	1870
StyD4I	CCNGG	6 cut(s)	81, 465, 471, 489, 760, 1526
StyI	CCWWGG	1 cut(s)	1192
TaaI	ACNGT	6 cut(s)	182, 208, 1033, 1151, 1334, 1691
TaiI	ACGT	3 cut(s)	261, 743, 1384
TaqI	TCGA	4 cut(s)	1035, 1329, 1408, 1802
TaqII	GACCGA	2 cut(s)	1109, 1721
TasI	AATT	9 cut(s)	894, 1046, 1081, 1249, 1364, 1401, 1446, 1569, 1769
TatI	WGTACW	2 cut(s)	567, 981
TauI	GCSGC	5 cut(s)	252, 255, 404, 509, 769
TfiI	GAWTC	4 cut(s)	535, 1294, 1413, 1433
Tru1I	TTAA	1 cut(s)	1209
Tru9I	TTAA	1 cut(s)	1209
TscAI	CASTG	5 cut(s)	587, 1681, 1787, 1968, 2050
TseFI	GTSAC	2 cut(s)	289, 408
TseI	GCWGC	7 cut(s)	321, 824, 954, 992, 995, 1070, 1712
Tsp45I	GTSAC	2 cut(s)	289, 408
TspDTI	ATGAA	5 cut(s)	17, 500, 1320, 1443, 2046
TspGWI	ACGGA	6 cut(s)	25, 83, 316, 449, 2010, 2014
TspRI	CASTG	5 cut(s)	587, 1681, 1787, 1968, 2050
Van91I	CCANNNNNTGG	1 cut(s)	472
VneI	GTGCAC	1 cut(s)	583
VpaK11BI	GGWCC	4 cut(s)	469, 592, 630, 1720
XagI	CCTNNNNNAGG	1 cut(s)	759
XapI	RAATTY	1 cut(s)	1249
XceI	RCATGY	3 cut(s)	427, 1498, 1975
XhoI	CTCGAG	1 cut(s)	1407
XmiI	GTMKAC	3 cut(s)	231, 714, 2052
XmnI	GAANNNNTTC	1 cut(s)	1293
XspI	CTAG	4 cut(s)	888, 963, 1664, 2058
ZraI	GACGTC	1 cut(s)	259
Zsp2I	ATGCAT	2 cut(s)	1500, 1826

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.