Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0000283

Physical Location & Seq

Genomic Coordinates Forward (+)

3248091 .. 3251110

Gene Structure 3020 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G003450.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1974 bp

ATGGCAGAAGTGATCCTTCTCTTCCTTTCAGTGGTCACCCTGCTAAGCTTTCCGACTACGGAAGCCGACTACCTCGCCCATATCTGCCCCAACACCACTGTCTTCACCCCAAACTCCACCTTCCAGTCCAACCTCAACCACCTCCTCTCCACTCTCTCCTCCAACGCCACACGCGACTCCGGCTTCTACAACGCCACCTCCGGCCGTACCTCTAACGACCCCGCCTACGGCCTCTTCCTCTGCCGCGGTGACGTCACCACCGCTGGTTGCAGAGCCTGCGTGACCACCGCAACCTCCGAGGTCGTGCGGCGCTGCCCGGTCGAGACAGAGGTTGTGATATGGTACGATGACTGCATGCTACGCTACTCCAACAGGTCCTTCTTCTCTGTCCCCGCCGAGTCGCCGATGTTGTTCATGTGGAACCCCCAAAACGTCAGCAGCGAAACGAACCGGTTCAACCAGGTTTTGGCGGCTAGCATGACGGAGGTGGCCACGGAGGCTTCGGGCGACGGCGATAAGTTCGCAACGAGAGAGGCGAACTTCACGGGGTTGATTAGCTTGTACAGCTTGGGGCAGTGCACGCAGGACTTGTCTACTTCTGATTGCAAACGGTGTCTTACGGGAGCCATTGCGAATTTGCCGAGTTGCTGCGGCGGGAAGTTAGGGGGACGAGTTCTGTATCCGAGTTGTAATGTTAGGTACGAAGTTTATCCCTTTTATAACTCGACGTCAGCACCGGCGCCGGCGCCAGGGGCTCGACCTCTTTCTCCGCCGAGCACAGTGACGAAAGGGAAGAGCAAAATCTCCTGGAAAAAGATTGTGGCCATTGCCGTTCCAATTGCAGCCTCTTTGGTGCTTTTTGTGATCGGCTACTGCCTCATTACTAAGAGGGCAAAAAAGAAGTACAATGCAGTACCAGAAACAAGTGAGAATGACATTTCTAGTGTAGAGTCTTTGCAATTTGATTTTGGTACCATTGAAGCTGCTACAAACAATTTCTCTGATGATAACAAGTTAGGCGAAGGTGGATTTGGTCAAGTCTACAAGGGTTTGCTTTCGGATGGTCAAGAAATAGCTGTCAAGAGGCTATCAAGGAACTCTGGCCAAGGTGCACAAGAATTTAAGAATGAGATGGTACTGGTAGCCAAGCTTCAACACCGAAATCTGGTCAGGCTATTGGGATTTTGCTTGGAAGGGGAAGAAAAGATGCTTGTTTATGAATACGTCCCCAACAAAAGCCTCGACTATATTCTATTTGACCCTGAGAAACAAGGACAGCTGGATTGGTCGAGACGTTACAAAATTATTGCGGGGATTGCCCGAGGAATTATGTATCTACATGAAGACTCCCGGCTAAGAATTATACATCGTGATCTTAAAGCCAGCAATATATTGTTAGATGGGGAAATGCATCCAAAAATATCTGATTTTGGCATGGCGAGGATTTTCGGTGTTGATCAAACTCAAGCAAACACAAATAGAATTGTTGGAACATATGGTTACATGTCTCCAGAATATGCAATGCATGGACAATTTTCTATCAAGTCCGATATCTATAGTTTTGGTGTCTTAGTTCTAGAAATTATCAGTGGCAAGAAGAATAGTTACTTCTTCCAAACAGATGCAGCTGAGGACCTCGTGAGCCATGCTTGGAAACTATGGAGAGATGGAACTCCTTTGGAATTTTTGGATCCGTGTCTGAGAGATTCTTATTCTAGAAATGAAGTTATCAGATGTATCCACATTGGCCTACTTTGTGTTCAGGAAGATCCGGCTGACAGACCTACAATGCAATCACTAGTTCTTATGCTCAACAGCTACTCTGTTACTCTACCATTACCTCAAGAGCCAGCATTTGTCCATAGGACTAGATCGGAGTTCATGCCAAAGATACCGCTGGAGTATGATCAGTCTATCAGCAAGTCAAGTCCATCGGTTTATGAAGGATCAATCACTGAAGTATATCCTCGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0042742 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050896 GO:0051704 GO:0051707 GO:0071704 GO:0071944 GO:0098542 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr Stress-antifung

Protein Analysis

657

Amino Acids

72.97

Weight (kDa)

6.13

Isoelectric Point (pI)

48.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Stress-antifung	PF01657	29 - 125	5e-28	Salt stress response/antifungal
Stress-antifung	PF01657	147 - 236	1.1e-11	Salt stress response/antifungal
Pkinase	PF00069	334 - 601	5e-46	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	336 - 602	1.1e-48	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0000283)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G45860 AT4G05200 AT4G05200 AT4G11521 AT4G11530 AT4G23130 AT4G23130 AT4G23130 AT4G23140 AT4G23140 AT4G23150 AT4G23160 AT4G23170 AT4G23180 AT4G23180 AT4G23180 AT4G23230 AT4G23270 AT4G23270 AT4G23270 AT4G23280 AT4G23280 AT4G23280 AT4G23310 AT4G23310
fragaria_vesca	FvH4_3g02772 FvH4_3g02772 FvH4_3g02773 FvH4_3g02773 FvH4_3g02774 FvH4_3g02800 FvH4_3g02820 FvH4_3g02820 FvH4_3g02830
malus_domestica	MD00G1101900.v1.1 MD00G1102100.v1.1 MD00G1102200.v1.1 MD01G1098200.v1.1 MD05G1340000.v1.1 MD05G1340300.v1.1 MD07G1014100.v1.1 MD07G1014300.v1.1 MD07G1014400.v1.1 MD07G1014700.v1.1 MD10G1312900.v1.1 MD11G1022600.v1.1 MD11G1291500.v1.1 MD14G1207100.v1.1 MD16G1096600.v1.1
prunus_persica	Prupe.4G027300_v2.0.a1 Prupe.4G027400_v2.0.a1 Prupe.4G027700_v2.0.a1 Prupe.4G027800_v2.0.a1
pyrus_communis	pycom05g30960 pycom10g00940 pycom10g00950 pycom10g26430 pycom10g26450 pycom10g26460 pycom10g26470 pycom10g26480 pycom10g26490 pycom10g26500 pycom10g26510 pycom10g26570 pycom11g01820 pycom11g04000 pycom14g17130 pycom14g17140 pycom15g28820
rosa_chinensis	RchiOBHm_Chr4g0408141 RchiOBHm_Chr5g0004211 RchiOBHm_Chr5g0004231 RchiOBHm_Chr5g0004241 RchiOBHm_Chr5g0004261
rosa_laevigata	RLG00000008668 RLG00000008672 RLG00000031212 RLG00000031217
rosa_multiflora	Rmu_co8162658.1_g000001 Rmu_co8336297.1_g000001 Rmu_sc0002073.1_g000001 Rmu_sc0004964.1_g000002 Rmu_sc0004964.1_g000003 Rmu_sc0004964.1_g000007 Rmu_sc0006006.1_g000007 Rmu_sc0007868.1_g000015 Rmu_sc0007868.1_g000016 Rmu_sc0007868.1_g000017 Rmu_sc0007868.1_g000021 Rmu_sc0007868.1_g000023 Rmu_sc0019654.1_g000002 Rmu_sc0038540.1_g000001
rosa_roxburghii	Rroxscaffold_1G00071450 Rroxscaffold_1G00071460 Rroxscaffold_1G00071470 Rroxscaffold_1G00071560 Rroxscaffold_1G00071720 Rroxscaffold_1G00071770 Rroxscaffold_5G00352640
rosa_rugosa	Rorug04G0083700 Rorug04G0410700 Rorug04G0410800 Rorug04G0410900
rosa_samantha	Rh4BG145400 Rh4CG154900 Rh4DG141900 Rh5BG037500 Rh5BG037600 Rh5BG037700 Rh5CG041000 Rh5CG041100 Rh5CG041200 Rh5CG041300 Rh5CG041400 Rh5DG037100
rosa_wichuraiana	Rw4G012130 Rw5G003450 Rw5G003460 Rw5G003470 Rw5G003480 Rw5G003640 Rw5G003660 Rw5G003670 Rw5G003680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	720
AatII	GACGTC	2 cut(s)	255, 731
Acc65I	GGTACC	1 cut(s)	971
AccB1I	GGYRCC	3 cut(s)	739, 745, 971
AccB7I	CCANNNNNTGG	1 cut(s)	466
AccI	GTMKAC	2 cut(s)	593, 1041
AccII	CGCG	2 cut(s)	174, 246
AclWI	GGATC	5 cut(s)	7, 1685, 1698, 1763, 1954
AcoI	YGGCCR	4 cut(s)	202, 489, 822, 1102
AcsI	RAATTY	3 cut(s)	634, 1118, 1682
AcyI	GRCGYC	4 cut(s)	252, 728, 740, 746
AfaI	GTAC	8 cut(s)	208, 344, 563, 701, 905, 915, 973, 1137
AfiI	CCNNNNNNNGG	5 cut(s)	31, 227, 466, 749, 1165
AflIII	ACRYGT	1 cut(s)	1503
AgeI	ACCGGT	1 cut(s)	450
AgsI	TTSAA	3 cut(s)	457, 980, 1154
AhlI	ACTAGT	1 cut(s)	1798
AjnI	CCWGG	3 cut(s)	459, 748, 806
AjuI	GAANNNNNNNTTGG	4 cut(s)	362, 394, 1014, 1046
AluBI	AGCT	9 cut(s)	48, 558, 567, 983, 1076, 1150, 1279, 1628, 1818
AluI	AGCT	9 cut(s)	48, 558, 567, 983, 1076, 1150, 1279, 1628, 1818
Alw21I	GWGCWC	3 cut(s)	581, 779, 1114
Alw26I	GTCTC	3 cut(s)	317, 1285, 1512
Alw44I	GTGCAC	2 cut(s)	577, 1110
AlwI	GGATC	5 cut(s)	7, 1685, 1698, 1763, 1954
AlwNI	CAGNNNCTG	1 cut(s)	276
Ama87I	CYCGRG	1 cut(s)	1320
AoxI	GGCC	6 cut(s)	202, 229, 489, 822, 1102, 1747
ApaLI	GTGCAC	2 cut(s)	577, 1110
ApeKI	GCWGC	6 cut(s)	312, 438, 648, 842, 983, 1625
ApoI	RAATTY	3 cut(s)	634, 1118, 1682
AsiGI	ACCGGT	1 cut(s)	450
Asp700I	GAANNNNTTC	1 cut(s)	452
Asp718I	GGTACC	1 cut(s)	971
AspLEI	GCGC	3 cut(s)	312, 742, 748
AspS9I	GGNCC	2 cut(s)	375, 1633
AsuC2I	CCSGG	2 cut(s)	317, 1351
AsuHPI	GGTGA	4 cut(s)	28, 97, 247, 260
AsuNHI	GCTAGC	1 cut(s)	473
AvaI	CYCGRG	1 cut(s)	1320
AvaII	GGWCC	2 cut(s)	375, 1633
BaeGI	GKGCMC	2 cut(s)	581, 1114
BalI	TGGCCA	3 cut(s)	491, 824, 1104
BamHI	GGATCC	1 cut(s)	1690
BanI	GGYRCC	3 cut(s)	739, 745, 971
BanII	GRGCYC	1 cut(s)	757
BarI	GAAGNNNNNNTAC	4 cut(s)	1134, 1166, 1589, 1621
BauI	CACGAG	1 cut(s)	1637
BbsI	GAAGAC	2 cut(s)	94, 1350
Bbv12I	GWGCWC	3 cut(s)	581, 779, 1114
BbvCI	CCTCAGC	1 cut(s)	1629
BbvI	GCAGC	6 cut(s)	299, 450, 635, 854, 970, 1637
BccI	CCATC	5 cut(s)	1055, 1126, 1394, 1661, 1939
BceAI	ACGGC	4 cut(s)	189, 244, 526, 815
BciT130I	CCWGG	3 cut(s)	461, 750, 808
BciVI	GTATCC	2 cut(s)	690, 1748
BclI	TGATCA	2 cut(s)	1456, 1906
BcnI	CCSGG	2 cut(s)	317, 1351
BcoDI	GTCTC	3 cut(s)	317, 1285, 1512
BcuI	ACTAGT	1 cut(s)	1798
BfaI	CTAG	6 cut(s)	474, 942, 1577, 1716, 1799, 1869
BfmI	CTRYAG	1 cut(s)	1555
BfoI	RGCGCY	3 cut(s)	313, 743, 749
BfuI	GTATCC	2 cut(s)	690, 1748
BglI	GCCNNNNNGGC	1 cut(s)	497
BlpI	GCTNAGC	1 cut(s)	44
Bme1390I	CCNGG	5 cut(s)	317, 461, 750, 808, 1351
Bme18I	GGWCC	2 cut(s)	375, 1633
BmeT110I	CYCGRG	1 cut(s)	1320
BmgT120I	GGNCC	2 cut(s)	375, 1633
BmiI	GGNNCC	6 cut(s)	422, 625, 741, 747, 973, 1692
BmrFI	CCNGG	5 cut(s)	317, 461, 750, 808, 1351
BmsI	GCATC	3 cut(s)	1197, 1420, 1612
BmtI	GCTAGC	1 cut(s)	477
BpiI	GAAGAC	2 cut(s)	94, 1350
BpmI	CTGGAG	2 cut(s)	1494, 1919
Bpu10I	CCTNAGC	1 cut(s)	1629
Bpu1102I	GCTNAGC	1 cut(s)	44
BpuEI	CTTGAG	2 cut(s)	1449, 1827
BpuMI	CCSGG	2 cut(s)	317, 1351
BsaHI	GRCGYC	4 cut(s)	252, 728, 740, 746
BsaJI	CCNNGG	6 cut(s)	244, 297, 492, 749, 1105, 1321
BsaWI	WCCGGW	1 cut(s)	450
BsaXI	ACNNNNNCTCC	4 cut(s)	131, 161, 182, 212
Bsc4I	CCNNNNNNNGG	5 cut(s)	31, 227, 466, 749, 1165
Bse118I	RCCGGY	3 cut(s)	450, 736, 742
Bse1I	ACTGG	2 cut(s)	124, 1143
Bse3DI	GCAATG	3 cut(s)	627, 825, 1527
BseBI	CCWGG	3 cut(s)	461, 750, 808
BseDI	CCNNGG	6 cut(s)	244, 297, 492, 749, 1105, 1321
BseGI	GGATG	2 cut(s)	1066, 1411
BseLI	CCNNNNNNNGG	5 cut(s)	31, 227, 466, 749, 1165
BseMI	GCAATG	3 cut(s)	627, 825, 1527
BseMII	CTCAG	3 cut(s)	1254, 1620, 1691
BseNI	ACTGG	2 cut(s)	124, 1143
BseRI	GAGGAG	2 cut(s)	134, 148
BseSI	GKGCMC	2 cut(s)	581, 1114
BseX3I	CGGCCG	1 cut(s)	202
BseXI	GCAGC	6 cut(s)	299, 450, 635, 854, 970, 1637
Bsh1236I	CGCG	2 cut(s)	174, 246
Bsh1285I	CGRYCG	2 cut(s)	205, 321
BshFI	GGCC	6 cut(s)	204, 231, 491, 824, 1104, 1749
BshNI	GGYRCC	3 cut(s)	739, 745, 971
BshTI	ACCGGT	1 cut(s)	450
BsiEI	CGRYCG	2 cut(s)	205, 321
BsiHKAI	GWGCWC	3 cut(s)	581, 779, 1114
BsiHKCI	CYCGRG	1 cut(s)	1320
BsiSI	CCGG	8 cut(s)	180, 201, 317, 451, 737, 743, 1351, 1772
BslFI	GGGAC	3 cut(s)	374, 681, 1211
BslI	CCNNNNNNNGG	5 cut(s)	31, 227, 466, 749, 1165
BsmAI	GTCTC	3 cut(s)	317, 1285, 1512
BsmBI	CGTCTC	1 cut(s)	1285
BsmFI	GGGAC	3 cut(s)	374, 681, 1211
BsnI	GGCC	6 cut(s)	204, 231, 491, 824, 1104, 1749
BsoBI	CYCGRG	1 cut(s)	1320
Bsp1286I	GDGCHC	4 cut(s)	581, 757, 779, 1114
Bsp1407I	TGTACA	1 cut(s)	561
Bsp143I	GATC	9 cut(s)	12, 864, 1372, 1456, 1690, 1768, 1871, 1906, 1946
Bsp1720I	GCTNAGC	1 cut(s)	44
BspANI	GGCC	6 cut(s)	204, 231, 491, 824, 1104, 1749
BspCNI	CTCAG	3 cut(s)	1255, 1621, 1692
BspFNI	CGCG	2 cut(s)	174, 246
BspLI	GGNNCC	6 cut(s)	422, 625, 741, 747, 973, 1692
BspOI	GCTAGC	1 cut(s)	477
BspPI	GGATC	5 cut(s)	7, 1685, 1698, 1763, 1954
BspQI	GCTCTTC	1 cut(s)	788
BspT107I	GGYRCC	3 cut(s)	739, 745, 971
BsrDI	GCAATG	3 cut(s)	627, 825, 1527
BsrFI	RCCGGY	3 cut(s)	450, 736, 742
BsrGI	TGTACA	1 cut(s)	561
BsrI	ACTGG	2 cut(s)	124, 1143
BssAI	RCCGGY	3 cut(s)	450, 736, 742
BssECI	CCNNGG	6 cut(s)	244, 297, 492, 749, 1105, 1321
BssMI	GATC	9 cut(s)	12, 864, 1372, 1456, 1690, 1768, 1871, 1906, 1946
BssNI	GRCGYC	4 cut(s)	252, 728, 740, 746
BssSI	CACGAG	1 cut(s)	1637
BssT1I	CCWWGG	1 cut(s)	1105
Bst2BI	CACGAG	1 cut(s)	1637
Bst2UI	CCWGG	3 cut(s)	461, 750, 808
Bst4CI	ACNGT	3 cut(s)	100, 612, 781
Bst6I	CTCTTC	3 cut(s)	26, 239, 788
BstACI	GRCGYC	4 cut(s)	252, 728, 740, 746
BstAPI	GCANNNNNTGC	1 cut(s)	276
BstAUI	TGTACA	1 cut(s)	561
BstC8I	GCNNGC	7 cut(s)	277, 356, 475, 581, 744, 1384, 1851
BstDEI	CTNAG	7 cut(s)	44, 885, 1263, 1355, 1570, 1629, 1700
BstDSI	CCRYGG	2 cut(s)	244, 492
BstEII	GGTNACC	1 cut(s)	34
BstF5I	GGATG	2 cut(s)	1066, 1411
BstFNI	CGCG	2 cut(s)	174, 246
BstH2I	RGCGCY	3 cut(s)	313, 743, 749
BstHHI	GCGC	3 cut(s)	312, 742, 748
BstKTI	GATC	9 cut(s)	15, 867, 1375, 1459, 1693, 1771, 1874, 1909, 1949
BstMAI	GTCTC	3 cut(s)	317, 1285, 1512
BstMBI	GATC	9 cut(s)	12, 864, 1372, 1456, 1690, 1768, 1871, 1906, 1946
BstMCI	CGRYCG	2 cut(s)	205, 321
BstMWI	GCNNNNNNNGC	8 cut(s)	180, 276, 360, 497, 564, 580, 752, 1316
BstNI	CCWGG	3 cut(s)	461, 750, 808
BstNSI	RCATGY	2 cut(s)	358, 1507
BstPI	GGTNACC	1 cut(s)	34
BstSCI	CCNGG	5 cut(s)	315, 459, 748, 806, 1349
BstSFI	CTRYAG	1 cut(s)	1555
BstSLI	GKGCMC	2 cut(s)	581, 1114
BstUI	CGCG	2 cut(s)	174, 246
BstV1I	GCAGC	6 cut(s)	299, 450, 635, 854, 970, 1637
BstV2I	GAAGAC	2 cut(s)	94, 1350
BstX2I	RGATCY	2 cut(s)	1690, 1768
BstYI	RGATCY	2 cut(s)	1690, 1768
BstZI	CGGCCG	1 cut(s)	202
BsuI	GTATCC	2 cut(s)	690, 1748
BsuRI	GGCC	6 cut(s)	204, 231, 491, 824, 1104, 1749
BtgI	CCRYGG	2 cut(s)	244, 492
BtsCI	GGATG	2 cut(s)	1066, 1411
BtsI	GCAGTG	1 cut(s)	581
BtsIMutI	CAGTG	6 cut(s)	36, 96, 581, 786, 1594, 1953
Cac8I	GCNNGC	7 cut(s)	277, 356, 475, 581, 744, 1384, 1851
CaiI	CAGNNNCTG	1 cut(s)	276
CfoI	GCGC	3 cut(s)	312, 742, 748
Cfr10I	RCCGGY	3 cut(s)	450, 736, 742
Cfr13I	GGNCC	2 cut(s)	375, 1633
Cfr42I	CCGCGG	1 cut(s)	247
CsiI	ACCWGGT	1 cut(s)	459
Csp6I	GTAC	8 cut(s)	207, 343, 562, 700, 904, 914, 972, 1136
CspAI	ACCGGT	1 cut(s)	450
CviAII	CATG	9 cut(s)	355, 415, 478, 1340, 1435, 1504, 1526, 1646, 1882
CviQI	GTAC	8 cut(s)	207, 343, 562, 700, 904, 914, 972, 1136
DdeI	CTNAG	7 cut(s)	44, 885, 1263, 1355, 1570, 1629, 1700
DinI	GGCGCC	2 cut(s)	741, 747
DpnI	GATC	9 cut(s)	14, 866, 1374, 1458, 1692, 1770, 1873, 1908, 1948
DpnII	GATC	9 cut(s)	12, 864, 1372, 1456, 1690, 1768, 1871, 1906, 1946
EaeI	YGGCCR	4 cut(s)	202, 489, 822, 1102
EagI	CGGCCG	1 cut(s)	202
Eam1104I	CTCTTC	3 cut(s)	26, 239, 788
EarI	CTCTTC	3 cut(s)	26, 239, 788
EciI	GGCGGA	1 cut(s)	759
EclXI	CGGCCG	1 cut(s)	202
Eco130I	CCWWGG	1 cut(s)	1105
Eco24I	GRGCYC	1 cut(s)	757
Eco32I	GATATC	1 cut(s)	1552
Eco47I	GGWCC	2 cut(s)	375, 1633
Eco52I	CGGCCG	1 cut(s)	202
Eco88I	CYCGRG	1 cut(s)	1320
Eco91I	GGTNACC	1 cut(s)	34
EcoO109I	RGGNCCY	2 cut(s)	375, 1633
EcoO65I	GGTNACC	1 cut(s)	34
EcoRII	CCWGG	3 cut(s)	459, 748, 806
EcoRV	GATATC	1 cut(s)	1552
EcoT14I	CCWWGG	1 cut(s)	1105
EcoT22I	ATGCAT	2 cut(s)	1413, 1527
EcoT38I	GRGCYC	1 cut(s)	757
EgeI	GGCGCC	2 cut(s)	741, 747
EheI	GGCGCC	2 cut(s)	741, 747
ErhI	CCWWGG	1 cut(s)	1105
Esp3I	CGTCTC	1 cut(s)	1285
FaeI	CATG	9 cut(s)	358, 418, 481, 1343, 1438, 1507, 1529, 1649, 1885
FalI	AAGNNNNNCTT	1 cut(s)	32
FaqI	GGGAC	3 cut(s)	374, 681, 1211
FatI	CATG	9 cut(s)	354, 414, 477, 1339, 1434, 1503, 1525, 1645, 1881
FauI	CCCGC	4 cut(s)	229, 400, 647, 1303
FauNDI	CATATG	1 cut(s)	1495
FbaI	TGATCA	2 cut(s)	1456, 1906
FblI	GTMKAC	2 cut(s)	593, 1041
FokI	GGATG	2 cut(s)	1073, 1398
FriOI	GRGCYC	1 cut(s)	757
FspBI	CTAG	6 cut(s)	474, 942, 1577, 1716, 1799, 1869
GlaI	GCGC	3 cut(s)	311, 741, 747
GsuI	CTGGAG	2 cut(s)	1494, 1919
HaeII	RGCGCY	3 cut(s)	313, 743, 749
HaeIII	GGCC	6 cut(s)	204, 231, 491, 824, 1104, 1749
HapII	CCGG	8 cut(s)	180, 201, 317, 451, 737, 743, 1351, 1772
HhaI	GCGC	3 cut(s)	312, 742, 748
Hin1I	GRCGYC	4 cut(s)	252, 728, 740, 746
Hin1II	CATG	9 cut(s)	358, 418, 481, 1343, 1438, 1507, 1529, 1649, 1885
Hin6I	GCGC	3 cut(s)	310, 740, 746
HinP1I	GCGC	3 cut(s)	310, 740, 746
HindIII	AAGCTT	2 cut(s)	46, 1148
HinfI	GANTC	5 cut(s)	176, 398, 950, 1346, 1706
HpaII	CCGG	8 cut(s)	180, 201, 317, 451, 737, 743, 1351, 1772
HphI	GGTGA	4 cut(s)	28, 97, 247, 260
Hpy166II	GTNNAC	4 cut(s)	579, 594, 1042, 1112
Hpy8I	GTNNAC	4 cut(s)	579, 594, 1042, 1112
Hpy99I	CGWCG	2 cut(s)	512, 730
HpyAV	CCTTC	6 cut(s)	26, 130, 388, 1016, 1187, 1937
HpyCH4III	ACNGT	3 cut(s)	100, 612, 781
HpyCH4IV	ACGT	5 cut(s)	252, 432, 728, 1224, 1294
HpyF10VI	GCNNNNNNNGC	8 cut(s)	180, 276, 360, 497, 564, 580, 752, 1316
HpyF3I	CTNAG	7 cut(s)	44, 885, 1263, 1355, 1570, 1629, 1700
HpySE526I	ACGT	5 cut(s)	252, 432, 728, 1224, 1294
Hsp92I	GRCGYC	4 cut(s)	252, 728, 740, 746
Hsp92II	CATG	9 cut(s)	358, 418, 481, 1343, 1438, 1507, 1529, 1649, 1885
HspAI	GCGC	3 cut(s)	310, 740, 746
KasI	GGCGCC	2 cut(s)	739, 745
KpnI	GGTACC	1 cut(s)	975
KroI	GCCGGC	1 cut(s)	742
KroNI	GCCGGC	1 cut(s)	744
Ksp22I	TGATCA	2 cut(s)	1456, 1906
KspI	CCGCGG	1 cut(s)	247
Kzo9I	GATC	9 cut(s)	12, 864, 1372, 1456, 1690, 1768, 1871, 1906, 1946
LguI	GCTCTTC	1 cut(s)	788
LmnI	GCTCC	1 cut(s)	623
Lsp1109I	GCAGC	6 cut(s)	299, 450, 635, 854, 970, 1637
LweI	GCATC	3 cut(s)	1197, 1420, 1612
MabI	ACCWGGT	1 cut(s)	459
MaeI	CTAG	6 cut(s)	474, 942, 1577, 1716, 1799, 1869
MaeII	ACGT	5 cut(s)	252, 432, 728, 1224, 1294
MaeIII	GTNAC	9 cut(s)	34, 248, 253, 280, 781, 1295, 1499, 1604, 1825
MalI	GATC	9 cut(s)	14, 866, 1374, 1458, 1692, 1770, 1873, 1908, 1948
MboI	GATC	9 cut(s)	12, 864, 1372, 1456, 1690, 1768, 1871, 1906, 1946
MfeI	CAATTG	1 cut(s)	837
MflI	RGATCY	2 cut(s)	1690, 1768
MhlI	GDGCHC	4 cut(s)	581, 757, 779, 1114
MlsI	TGGCCA	3 cut(s)	491, 824, 1104
MluNI	TGGCCA	3 cut(s)	491, 824, 1104
Mly113I	GGCGCC	2 cut(s)	740, 746
MlyI	GAGTC	4 cut(s)	170, 407, 959, 1340
MmeI	TCCRAC	5 cut(s)	77, 153, 186, 393, 1468
Mox20I	TGGCCA	3 cut(s)	491, 824, 1104
Mph1103I	ATGCAT	2 cut(s)	1413, 1527
MreI	CGCCGGCG	1 cut(s)	742
MroNI	GCCGGC	1 cut(s)	742
MroXI	GAANNNNTTC	1 cut(s)	452
MscI	TGGCCA	3 cut(s)	491, 824, 1104
MseI	TTAA	2 cut(s)	1122, 1377
Msp20I	TGGCCA	3 cut(s)	491, 824, 1104
MspA1I	CMGCKG	5 cut(s)	246, 263, 1279, 1628, 1897
MspI	CCGG	8 cut(s)	180, 201, 317, 451, 737, 743, 1351, 1772
MspR9I	CCNGG	5 cut(s)	317, 461, 750, 808, 1351
MunI	CAATTG	1 cut(s)	837
MvaI	CCWGG	3 cut(s)	461, 750, 808
MvnI	CGCG	2 cut(s)	174, 246
MwoI	GCNNNNNNNGC	8 cut(s)	180, 276, 360, 497, 564, 580, 752, 1316
NaeI	GCCGGC	1 cut(s)	744
NarI	GGCGCC	2 cut(s)	740, 746
NciI	CCSGG	2 cut(s)	317, 1351
NdeI	CATATG	1 cut(s)	1495
NdeII	GATC	9 cut(s)	12, 864, 1372, 1456, 1690, 1768, 1871, 1906, 1946
NgoMIV	GCCGGC	1 cut(s)	742
NheI	GCTAGC	1 cut(s)	473
NlaIII	CATG	9 cut(s)	358, 418, 481, 1343, 1438, 1507, 1529, 1649, 1885
NlaIV	GGNNCC	6 cut(s)	422, 625, 741, 747, 973, 1692
NmeAIII	GCCGAG	3 cut(s)	421, 666, 798
NmuCI	GTSAC	5 cut(s)	34, 248, 253, 280, 781
NsiI	ATGCAT	2 cut(s)	1413, 1527
NspI	RCATGY	2 cut(s)	358, 1507
PaeI	GCATGC	1 cut(s)	358
PciI	ACATGT	1 cut(s)	1503
PciSI	GCTCTTC	1 cut(s)	788
PcsI	WCGNNNNNNNCGW	3 cut(s)	171, 438, 533
PdiI	GCCGGC	1 cut(s)	744
PdmI	GAANNNNTTC	1 cut(s)	452
PfeI	GAWTC	1 cut(s)	1706
PflMI	CCANNNNNTGG	1 cut(s)	466
PfoI	TCCNGGA	1 cut(s)	806
PinAI	ACCGGT	1 cut(s)	450
PleI	GAGTC	4 cut(s)	170, 406, 958, 1340
PluTI	GGCGCC	2 cut(s)	743, 749
PpsI	GAGTC	4 cut(s)	170, 406, 958, 1340
PpuMI	RGGWCCY	2 cut(s)	375, 1633
PscI	ACATGT	1 cut(s)	1503
PsiI	TTATAA	1 cut(s)	720
Psp5II	RGGWCCY	2 cut(s)	375, 1633
Psp6I	CCWGG	3 cut(s)	459, 748, 806
PspEI	GGTNACC	1 cut(s)	34
PspGI	CCWGG	3 cut(s)	459, 748, 806
PspN4I	GGNNCC	6 cut(s)	422, 625, 741, 747, 973, 1692
PspPI	GGNCC	2 cut(s)	375, 1633
PspPPI	RGGWCCY	2 cut(s)	375, 1633
PstNI	CAGNNNCTG	1 cut(s)	276
PsuI	RGATCY	2 cut(s)	1690, 1768
PvuII	CAGCTG	2 cut(s)	1279, 1628
RsaI	GTAC	8 cut(s)	208, 344, 563, 701, 905, 915, 973, 1137
RsaNI	GTAC	8 cut(s)	207, 343, 562, 700, 904, 914, 972, 1136
SacII	CCGCGG	1 cut(s)	247
SapI	GCTCTTC	1 cut(s)	788
SaqAI	TTAA	2 cut(s)	1122, 1377
Sau3AI	GATC	9 cut(s)	12, 864, 1372, 1456, 1690, 1768, 1871, 1906, 1946
Sau96I	GGNCC	2 cut(s)	375, 1633
SchI	GAGTC	4 cut(s)	170, 407, 959, 1340
ScrFI	CCNGG	5 cut(s)	317, 461, 750, 808, 1351
SduI	GDGCHC	4 cut(s)	581, 757, 779, 1114
SexAI	ACCWGGT	1 cut(s)	459
SfaNI	GCATC	3 cut(s)	1197, 1420, 1612
SfcI	CTRYAG	1 cut(s)	1555
SfoI	GGCGCC	2 cut(s)	741, 747
Sfr303I	CCGCGG	1 cut(s)	247
SgrAI	CRCCGGYG	2 cut(s)	736, 742
SgrBI	CCGCGG	1 cut(s)	247
SinI	GGWCC	2 cut(s)	375, 1633
SmlI	CTYRAG	2 cut(s)	1464, 1842
SmoI	CTYRAG	2 cut(s)	1464, 1842
SpeI	ACTAGT	1 cut(s)	1798
SphI	GCATGC	1 cut(s)	358
SspDI	GGCGCC	2 cut(s)	739, 745
SspMI	CTAG	6 cut(s)	474, 942, 1577, 1716, 1799, 1869
StyD4I	CCNGG	5 cut(s)	315, 459, 748, 806, 1349
StyI	CCWWGG	1 cut(s)	1105
TaaI	ACNGT	3 cut(s)	100, 612, 781
TaiI	ACGT	5 cut(s)	255, 435, 731, 1227, 1297
TaqI	TCGA	6 cut(s)	321, 725, 757, 1242, 1289, 1969
TatI	WGTACW	2 cut(s)	561, 903
TauI	GCSGC	4 cut(s)	246, 310, 473, 654
TfiI	GAWTC	1 cut(s)	1706
Tru1I	TTAA	2 cut(s)	1122, 1377
Tru9I	TTAA	2 cut(s)	1122, 1377
TscAI	CASTG	6 cut(s)	36, 103, 581, 786, 1594, 1960
TseFI	GTSAC	5 cut(s)	34, 248, 253, 280, 781
TseI	GCWGC	6 cut(s)	312, 438, 648, 842, 983, 1625
Tsp45I	GTSAC	5 cut(s)	34, 248, 253, 280, 781
TspDTI	ATGAA	6 cut(s)	403, 1233, 1356, 1737, 1870, 1956
TspGWI	ACGGA	4 cut(s)	74, 497, 509, 1683
TspRI	CASTG	6 cut(s)	36, 103, 581, 786, 1594, 1960
Van91I	CCANNNNNTGG	1 cut(s)	466
VneI	GTGCAC	2 cut(s)	577, 1110
VpaK11BI	GGWCC	2 cut(s)	375, 1633
XapI	RAATTY	3 cut(s)	634, 1118, 1682
XbaI	TCTAGA	2 cut(s)	1576, 1715
XceI	RCATGY	2 cut(s)	358, 1507
XmiI	GTMKAC	2 cut(s)	593, 1041
XmnI	GAANNNNTTC	1 cut(s)	452
XspI	CTAG	6 cut(s)	474, 942, 1577, 1716, 1799, 1869
ZraI	GACGTC	2 cut(s)	253, 729
Zsp2I	ATGCAT	2 cut(s)	1413, 1527

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.