MD07G1014700.v1.1

Salt stress response/antifungal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0000283
Physical Location & Seq
Reverse (-)
1321764 .. 1326411
4648 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1014700.v1.1.491

Sequence Viewer

Length: 2658 bp
ATGAAAAACATGACTTCCGTTCTTTTGATTTTCTCCATTTTTACTCTGCCCAGCTTTCTGACCAGTACGGAAGCAGAATATCTCTACCACGTATGCCCAAACACCACCACCTTCACCCCAAACTCCACCTTCCAGTCCAATCTCAACCGCCTCTTCTCCACCCTCTCCTCCAACGCCTACCGCTCCACCGGCTTCTACAACACCTCCGTCCAAACCCCAAACAACGCCGTCTACGGTCTCTTCCTCTGCCGCGGTGATGTCTTGGGAACCGACGCTTGTGAAACCTGCGTTGCCAATGCAACCAGCGAGGCTCCCCAACGATGCCCCATCGAAAAAGAGGCTGTGATCTGGTATGACGACTGCATGCTGCGCTACTCGTACGTGTCATTCTTTTCCACCTCGGCCGAGAACCCTGGCGTGTTCCTGATGAACACGCAGAACGTAACGGAACAGACTCGGTTCAACCAGGTGCTGGCGGCGAGTATGAACGAGGTGGCTACCGAGGCTGCCAACGACGCCTACAAGTTCGCGACAAAACAAACGAAATTCAGTGATCTGATTACGTTGTACAGCCTCGCGCAGTGCACGCAGGACCTGTCCGCGGCCGTTTGCGACGAGTGCCTTAGGGATGTCATAGCGCTATTGCCGTCTTGTTGTAGCGGAAAGCAAGGGGGACGAGTTCTGCTTCCGAGTTGTAGCGTTAGGTACGAAATCTACCCCTTCTACACCCAGAACGCCACAGCACATGAGCCCACCCCTACCAAAGCTGTTTACCTGGACGAGTACTGCCCACCCACCACCACCTTCACCCCAAACTCCGCCTTCCAATCCAACCTCAAACGCCTCCTATCCACCCTCTCCTCCGAAGCCACGCGTTACACCGGCTTCTACAATTACACCGCATCCTCTTCCCAAAACCCTTATGACACTGTGTACGGCCTCTTCCTTTGCCGCGGCGACTTGGTCTCCACTGACGAGTGCAAAACCTGCGTTGCCACCGCAACGTCGGAGGCCCTCGAGCGCTGCCCCAATAGAAAAAATGTTATAATCTGGTACGTCGACTGCATGTTGCGCTACTCTGACGAGTCCTTCTTTTCCAGACTTGACGACATCCCTTTAATGTACTTGGGGAACAAGACGAAGGTAACGGCCAACGAAAGCCGCTTTTACCAGGTACTGGAGGCGAGTGTGAATGAGTTGGCCGTTCAGGCTTCCAATAAAAGCAATATGTTTGCAACCAAAAAAGCGGACGTTTCAGGGTCCATTTCGCTGCAAGTGCTTGGGCAGTGCACGCAGGACTTGTCCCCTAGAGATTGCTATTTATGTCTTCAGGGAGCCATAGCAGAAGTTCCCAGAATACAACAAGGGGGACGACTTCTATGTCCCAGTTGCCGTCTTACGATTGAAGTTTACCGCTACATCCTCCGCACCCCCTCCTTCCCACCTCCGCTTCCTCCTCCGCCAGCCTCCATCAGGGCAAGGCCTAAAGGAAGAAGCAGATTATCTCCTTACATAATCATTATTGGCAGTATTCTTGCTTTTATTGCTGTTACGGTCGTATTAGTCTACTGCTTTGCAAGCAGAAGAGCAGTAAGAAATAATGCTCCAAGTCAAGAAAACGTTAGGGACGACACAGAGACTCTTGAGTCCTTGCAATTCGACTTGGGAACCATTGAAACCGCTACAAACAAGTTCTCGGACAATAACAAGTTAGGTGAAGGCGGATTTGGTGTAGTTTTTAAGGGAACACTTGCTAATGAACAAGAAATAGCAGTGAAGAGGTTGTCAAAGAGCTCCAAGCAAGGTGTACAAGAATTTAAGAATGAGGTTGCATTGGTAGCCAAACTTCAACACAGAAATCTTGTTAGGCTTCTGGGATTTTGTTTGGAAGGAGAGGAAACCATACTTGTTTATGAATATGTGCCTAACCAAAGTCTTGATTATTTTCTTTTTGAATCCAAGAAACGTGAACAGCTGGATTGGTCAAGGCGTTGCATGATAATAGGAGGAATCACTCAAGGAATTGTATATCTGCATGAATATTCAAGGCTTAGAGTTATACATCGTGATTTAAAAGCGAGTAACATTCTGTTAGATGAAAATATGAATCCAAAAATATCAGATTTTGGTATGGCAAGAATGTTTGGAGCCGATGATCAAACTCAAGGAAACACCGAAAGAATTGTCGGCACCTATGGTTACATGGCTCCAGAATATGCAATGGAGGGATTGTATTCAATAAAATCTGATGTATTTAGCTTCGGAGTACTTTTACTTGAGATCATAACGGGGAGAAAGAACTTTTTAGGTTTTCATCTGACAAATGGTGCACCGACACTTATAGGAAACGCTTGGAAATTATGGAATGAAGGGAAAGTGTTGGAGTTGATGGATCCGTTGTTGAAAGGCTCATGCAGTCCAAATGAATTCCTGAGATACGTTCATATCGGATTATTGTGTGTTCAAGAAGATGCAAACAACAGGCCGACCATGTCATCAGTTGTTCTTATGTTGAAAAGTGAAACCATTGGTCTTTCTAAACCTGAGCGACCAGCCTTCTTTACAGGGAGATGTATTGATCACCATGATCAAGTAGCAGTAAGTGCTCACAATTGCTCGGTCAATGGCTTGACGATTTCTAATGTTGTGGCCCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr Stress-antifung

Protein Analysis

886

Amino Acids

98.67

Weight (kDa)

6.29

Isoelectric Point (pI)

42.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stress-antifung PF01657 32 - 126 1.7e-26 Salt stress response/antifungal
Stress-antifung PF01657 146 - 238 1.2e-11 Salt stress response/antifungal
Stress-antifung PF01657 263 - 361 3.2e-25 Salt stress response/antifungal
Stress-antifung PF01657 381 - 469 3.3e-07 Salt stress response/antifungal
Pkinase PF00069 566 - 769 4.3e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 568 - 835 4e-50 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000283)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G45860 AT4G05200 AT4G05200 AT4G11521 AT4G11530 AT4G23130 AT4G23130 AT4G23130 AT4G23140 AT4G23140 AT4G23150 AT4G23160 AT4G23170 AT4G23180 AT4G23180 AT4G23180 AT4G23230 AT4G23270 AT4G23270 AT4G23270 AT4G23280 AT4G23280 AT4G23280 AT4G23310 AT4G23310
fragaria_vesca FvH4_3g02772 FvH4_3g02772 FvH4_3g02773 FvH4_3g02773 FvH4_3g02774 FvH4_3g02800 FvH4_3g02820 FvH4_3g02820 FvH4_3g02830
malus_domestica MD00G1101900.v1.1 MD00G1102100.v1.1 MD00G1102200.v1.1 MD01G1098200.v1.1 MD05G1340000.v1.1 MD05G1340300.v1.1 MD07G1014100.v1.1 MD07G1014300.v1.1 MD07G1014400.v1.1 MD07G1014700.v1.1 MD10G1312900.v1.1 MD11G1022600.v1.1 MD11G1291500.v1.1 MD14G1207100.v1.1 MD16G1096600.v1.1
prunus_persica Prupe.4G027300_v2.0.a1 Prupe.4G027400_v2.0.a1 Prupe.4G027700_v2.0.a1 Prupe.4G027800_v2.0.a1
pyrus_communis pycom05g30960 pycom10g00940 pycom10g00950 pycom10g26430 pycom10g26450 pycom10g26460 pycom10g26470 pycom10g26480 pycom10g26490 pycom10g26500 pycom10g26510 pycom10g26570 pycom11g01820 pycom11g04000 pycom14g17130 pycom14g17140 pycom15g28820
rosa_chinensis RchiOBHm_Chr4g0408141 RchiOBHm_Chr5g0004211 RchiOBHm_Chr5g0004231 RchiOBHm_Chr5g0004241 RchiOBHm_Chr5g0004261
rosa_laevigata RLG00000008668 RLG00000008672 RLG00000031212 RLG00000031217
rosa_multiflora Rmu_co8162658.1_g000001 Rmu_co8336297.1_g000001 Rmu_sc0002073.1_g000001 Rmu_sc0004964.1_g000002 Rmu_sc0004964.1_g000003 Rmu_sc0004964.1_g000007 Rmu_sc0006006.1_g000007 Rmu_sc0007868.1_g000015 Rmu_sc0007868.1_g000016 Rmu_sc0007868.1_g000017 Rmu_sc0007868.1_g000021 Rmu_sc0007868.1_g000023 Rmu_sc0019654.1_g000002 Rmu_sc0038540.1_g000001
rosa_roxburghii Rroxscaffold_1G00071450 Rroxscaffold_1G00071460 Rroxscaffold_1G00071470 Rroxscaffold_1G00071560 Rroxscaffold_1G00071720 Rroxscaffold_1G00071770 Rroxscaffold_5G00352640
rosa_rugosa Rorug04G0083700 Rorug04G0410700 Rorug04G0410800 Rorug04G0410900
rosa_samantha Rh4BG145400 Rh4CG154900 Rh4DG141900 Rh5BG037500 Rh5BG037600 Rh5BG037700 Rh5CG041000 Rh5CG041100 Rh5CG041200 Rh5CG041300 Rh5CG041400 Rh5DG037100
rosa_wichuraiana Rw4G012130 Rw5G003450 Rw5G003460 Rw5G003470 Rw5G003480 Rw5G003640 Rw5G003660 Rw5G003670 Rw5G003680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1046
AasI GACNNNNNNGTC 2 cut(s) 1380, 1645
Acc36I ACCTGC 2 cut(s) 293, 995
AccB1I GGYRCC 1 cut(s) 2189
AccB7I CCANNNNNTGG 2 cut(s) 472, 1177
AccBSI CCGCTC 1 cut(s) 183
AccI GTMKAC 3 cut(s) 231, 1059, 1566
AccII CGCG 6 cut(s) 252, 530, 578, 602, 874, 954
AclI AACGTT 1 cut(s) 1620
AclWI GGATC 2 cut(s) 2387, 2400
AcoI YGGCCR 4 cut(s) 402, 603, 1149, 1200
AcsI RAATTY 3 cut(s) 545, 1814, 2426
AcuI CTGAAG 1 cut(s) 1313
AcyI GRCGYC 1 cut(s) 516
AfeI AGCGCT 2 cut(s) 639, 1022
AfiI CCNNNNNNNGG 4 cut(s) 472, 601, 1177, 1473
AflIII ACRYGT 2 cut(s) 381, 872
AjnI CCWGG 4 cut(s) 412, 465, 774, 1170
AjuI GAANNNNNNNTTGG 2 cut(s) 1710, 1742
AluBI AGCT 5 cut(s) 54, 767, 1794, 1975, 2259
AluI AGCT 5 cut(s) 54, 767, 1794, 1975, 2259
Alw21I GWGCWC 5 cut(s) 587, 1292, 1796, 2332, 2608
Alw26I GTCTC 3 cut(s) 242, 970, 1631
Alw44I GTGCAC 3 cut(s) 583, 1288, 2328
AlwI GGATC 2 cut(s) 2387, 2400
AlwNI CAGNNNCTG 3 cut(s) 472, 595, 1177
Ama87I CYCGRG 1 cut(s) 1016
Aor51HI AGCGCT 2 cut(s) 639, 1022
AoxI GGCC 9 cut(s) 402, 603, 937, 1011, 1149, 1200, 1481, 2483, 2649
ApaLI GTGCAC 3 cut(s) 583, 1288, 2328
ApeKI GCWGC 4 cut(s) 367, 506, 1023, 1270
ApoI RAATTY 3 cut(s) 545, 1814, 2426
ArsI GACNNNNNNTTYG 2 cut(s) 2169, 2201
AspLEI GCGC 5 cut(s) 372, 580, 640, 1023, 1074
AspS9I GGNCC 4 cut(s) 592, 1012, 1260, 2650
AsuHPI GGTGA 5 cut(s) 106, 266, 799, 1727, 2573
AvaI CYCGRG 1 cut(s) 1016
AvaII GGWCC 2 cut(s) 592, 1260
AxyI CCTNAGG 1 cut(s) 623
BaeGI GKGCMC 3 cut(s) 587, 1292, 2332
BamHI GGATCC 1 cut(s) 2392
BanI GGYRCC 1 cut(s) 2189
BanII GRGCYC 2 cut(s) 753, 1796
BarI GAAGNNNNNNTAC 4 cut(s) 926, 958, 1830, 1862
BbsI GAAGAC 1 cut(s) 1319
Bbv12I GWGCWC 5 cut(s) 587, 1292, 1796, 2332, 2608
BbvI GCAGC 4 cut(s) 354, 493, 1010, 1257
BccI CCATC 3 cut(s) 335, 1478, 2383
BceAI ACGGC 7 cut(s) 212, 590, 631, 952, 1164, 1187, 1377
BciT130I CCWGG 4 cut(s) 414, 467, 776, 1172
BclI TGATCA 3 cut(s) 2155, 2578, 2587
BcoDI GTCTC 3 cut(s) 242, 970, 1631
BfaI CTAG 1 cut(s) 1308
BfoI RGCGCY 2 cut(s) 641, 1024
BfuAI ACCTGC 2 cut(s) 293, 995
BglI GCCNNNNNGGC 1 cut(s) 1208
BmcAI AGTACT 2 cut(s) 785, 2268
Bme1390I CCNGG 4 cut(s) 414, 467, 776, 1172
Bme18I GGWCC 2 cut(s) 592, 1260
BmeT110I CYCGRG 1 cut(s) 1016
BmgT120I GGNCC 4 cut(s) 592, 1012, 1260, 2650
BmiI GGNNCC 9 cut(s) 268, 312, 1261, 1336, 1669, 2149, 2191, 2208, 2394
BmrFI CCNGG 4 cut(s) 414, 467, 776, 1172
BmrI ACTGGG 1 cut(s) 1380
BmsI GCATC 3 cut(s) 311, 911, 2461
BmuI ACTGGG 1 cut(s) 1380
BpiI GAAGAC 1 cut(s) 1319
BpmI CTGGAG 2 cut(s) 1199, 2193
Bpu10I CCTNAGC 1 cut(s) 2544
BpuEI CTTGAG 4 cut(s) 1664, 2001, 2148, 2297
BsaAI YACGTR 2 cut(s) 91, 382
BsaHI GRCGYC 1 cut(s) 516
BsaI GGTCTC 2 cut(s) 242, 970
BsaJI CCNNGG 6 cut(s) 250, 399, 412, 501, 600, 952
BsaXI ACNNNNNCTCC 6 cut(s) 188, 218, 950, 980, 2216, 2246
Bsc4I CCNNNNNNNGG 4 cut(s) 472, 601, 1177, 1473
Bse118I RCCGGY 2 cut(s) 188, 881
Bse1I ACTGG 4 cut(s) 63, 133, 1182, 1386
Bse21I CCTNAGG 1 cut(s) 623
Bse3DI GCAATG 1 cut(s) 2226
BseBI CCWGG 4 cut(s) 414, 467, 776, 1172
BseDI CCNNGG 6 cut(s) 250, 399, 412, 501, 600, 952
BseGI GGATG 4 cut(s) 634, 902, 1110, 1419
BseLI CCNNNNNNNGG 4 cut(s) 472, 601, 1177, 1473
BseMI GCAATG 1 cut(s) 2226
BseMII CTCAG 2 cut(s) 2423, 2535
BseNI ACTGG 4 cut(s) 63, 133, 1182, 1386
BseRI GAGGAG 3 cut(s) 157, 850, 1446
BseSI GKGCMC 3 cut(s) 587, 1292, 2332
BseX3I CGGCCG 2 cut(s) 402, 603
BseXI GCAGC 4 cut(s) 354, 493, 1010, 1257
BseYI CCCAGC 1 cut(s) 50
Bsh1236I CGCG 6 cut(s) 252, 530, 578, 602, 874, 954
Bsh1285I CGRYCG 3 cut(s) 405, 606, 1557
BshFI GGCC 9 cut(s) 404, 605, 939, 1013, 1151, 1202, 1483, 2485, 2651
BshNI GGYRCC 1 cut(s) 2189
BsiEI CGRYCG 3 cut(s) 405, 606, 1557
BsiHKAI GWGCWC 5 cut(s) 587, 1292, 1796, 2332, 2608
BsiHKCI CYCGRG 1 cut(s) 1016
BsiSI CCGG 2 cut(s) 189, 882
BsiWI CGTACG 1 cut(s) 378
BslFI GGGAC 5 cut(s) 687, 1288, 1368, 1383, 1640
BslI CCNNNNNNNGG 4 cut(s) 472, 601, 1177, 1473
BsmAI GTCTC 3 cut(s) 242, 970, 1631
BsmFI GGGAC 5 cut(s) 687, 1288, 1368, 1383, 1640
BsnI GGCC 9 cut(s) 404, 605, 939, 1013, 1151, 1202, 1483, 2485, 2651
Bso31I GGTCTC 2 cut(s) 242, 970
BsoBI CYCGRG 1 cut(s) 1016
Bsp1286I GDGCHC 6 cut(s) 587, 753, 1292, 1796, 2332, 2608
Bsp1407I TGTACA 2 cut(s) 567, 1807
Bsp143I GATC 7 cut(s) 345, 553, 2155, 2280, 2392, 2578, 2587
Bsp68I TCGCGA 1 cut(s) 530
BspANI GGCC 9 cut(s) 404, 605, 939, 1013, 1151, 1202, 1483, 2485, 2651
BspCNI CTCAG 2 cut(s) 2424, 2536
BspFNI CGCG 6 cut(s) 252, 530, 578, 602, 874, 954
BspLI GGNNCC 9 cut(s) 268, 312, 1261, 1336, 1669, 2149, 2191, 2208, 2394
BspMI ACCTGC 2 cut(s) 293, 995
BspPI GGATC 2 cut(s) 2387, 2400
BspQI GCTCTTC 1 cut(s) 1579
BspT107I GGYRCC 1 cut(s) 2189
BspTNI GGTCTC 2 cut(s) 242, 970
BsrBI CCGCTC 1 cut(s) 183
BsrDI GCAATG 1 cut(s) 2226
BsrFI RCCGGY 2 cut(s) 188, 881
BsrGI TGTACA 2 cut(s) 567, 1807
BsrI ACTGG 4 cut(s) 63, 133, 1182, 1386
BssAI RCCGGY 2 cut(s) 188, 881
BssECI CCNNGG 6 cut(s) 250, 399, 412, 501, 600, 952
BssMI GATC 7 cut(s) 345, 553, 2155, 2280, 2392, 2578, 2587
BssNI GRCGYC 1 cut(s) 516
Bst2UI CCWGG 4 cut(s) 414, 467, 776, 1172
Bst4CI ACNGT 3 cut(s) 236, 931, 1555
Bst6I CTCTTC 6 cut(s) 158, 245, 913, 947, 1579, 1772
BstACI GRCGYC 1 cut(s) 516
BstAPI GCANNNNNTGC 2 cut(s) 987, 2603
BstAUI TGTACA 2 cut(s) 567, 1807
BstBAI YACGTR 2 cut(s) 91, 382
BstC8I GCNNGC 6 cut(s) 365, 474, 587, 1292, 1464, 1579
BstDEI CTNAG 4 cut(s) 623, 2051, 2432, 2544
BstDSI CCRYGG 3 cut(s) 250, 600, 952
BstENI CCTNNNNNAGG 1 cut(s) 1471
BstF5I GGATG 4 cut(s) 634, 902, 1110, 1419
BstFNI CGCG 6 cut(s) 252, 530, 578, 602, 874, 954
BstH2I RGCGCY 2 cut(s) 641, 1024
BstHHI GCGC 5 cut(s) 372, 580, 640, 1023, 1074
BstKTI GATC 7 cut(s) 348, 556, 2158, 2283, 2395, 2581, 2590
BstMAI GTCTC 3 cut(s) 242, 970, 1631
BstMBI GATC 7 cut(s) 345, 553, 2155, 2280, 2392, 2578, 2587
BstMCI CGRYCG 3 cut(s) 405, 606, 1557
BstNI CCWGG 4 cut(s) 414, 467, 776, 1172
BstNSI RCATGY 2 cut(s) 367, 1069
BstSCI CCNGG 4 cut(s) 412, 465, 774, 1170
BstSLI GKGCMC 3 cut(s) 587, 1292, 2332
BstUI CGCG 6 cut(s) 252, 530, 578, 602, 874, 954
BstV1I GCAGC 4 cut(s) 354, 493, 1010, 1257
BstV2I GAAGAC 1 cut(s) 1319
BstX2I RGATCY 1 cut(s) 2392
BstYI RGATCY 1 cut(s) 2392
BstZI CGGCCG 2 cut(s) 402, 603
Bsu36I CCTNAGG 1 cut(s) 623
BsuRI GGCC 9 cut(s) 404, 605, 939, 1013, 1151, 1202, 1483, 2485, 2651
BtgI CCRYGG 3 cut(s) 250, 600, 952
BtsCI GGATG 4 cut(s) 634, 902, 1110, 1419
BtsI GCAGTG 3 cut(s) 587, 1292, 1779
BtsIMutI CAGTG 6 cut(s) 556, 587, 927, 969, 1292, 1779
BtuMI TCGCGA 1 cut(s) 530
BveI ACCTGC 2 cut(s) 293, 995
Cac8I GCNNGC 6 cut(s) 365, 474, 587, 1292, 1464, 1579
CaiI CAGNNNCTG 3 cut(s) 472, 595, 1177
CfoI GCGC 5 cut(s) 372, 580, 640, 1023, 1074
Cfr10I RCCGGY 2 cut(s) 188, 881
Cfr13I GGNCC 4 cut(s) 592, 1012, 1260, 2650
Cfr42I CCGCGG 3 cut(s) 253, 603, 955
CseI GACGC 2 cut(s) 281, 524
CsiI ACCWGGT 2 cut(s) 465, 1170
DdeI CTNAG 4 cut(s) 623, 2051, 2432, 2544
DpnI GATC 7 cut(s) 347, 555, 2157, 2282, 2394, 2580, 2589
DpnII GATC 7 cut(s) 345, 553, 2155, 2280, 2392, 2578, 2587
DraI TTTAAA 1 cut(s) 2073
DrdI GACNNNNNNGTC 2 cut(s) 1380, 1645
DseDI GACNNNNNNGTC 2 cut(s) 1380, 1645
EaeI YGGCCR 4 cut(s) 402, 603, 1149, 1200
EagI CGGCCG 2 cut(s) 402, 603
Eam1104I CTCTTC 6 cut(s) 158, 245, 913, 947, 1579, 1772
EarI CTCTTC 6 cut(s) 158, 245, 913, 947, 1579, 1772
EciI GGCGGA 3 cut(s) 808, 1449, 1737
Ecl136II GAGCTC 1 cut(s) 1794
EclXI CGGCCG 2 cut(s) 402, 603
Eco147I AGGCCT 1 cut(s) 1483
Eco24I GRGCYC 2 cut(s) 753, 1796
Eco31I GGTCTC 2 cut(s) 242, 970
Eco47I GGWCC 2 cut(s) 592, 1260
Eco47III AGCGCT 2 cut(s) 639, 1022
Eco52I CGGCCG 2 cut(s) 402, 603
Eco53kI GAGCTC 1 cut(s) 1794
Eco57I CTGAAG 1 cut(s) 1313
Eco81I CCTNAGG 1 cut(s) 623
Eco88I CYCGRG 1 cut(s) 1016
EcoICRI GAGCTC 1 cut(s) 1794
EcoNI CCTNNNNNAGG 1 cut(s) 1471
EcoO109I RGGNCCY 2 cut(s) 592, 1012
EcoRI GAATTC 1 cut(s) 2426
EcoRII CCWGG 4 cut(s) 412, 465, 774, 1170
EcoT38I GRGCYC 2 cut(s) 753, 1796
FaqI GGGAC 5 cut(s) 687, 1288, 1368, 1383, 1640
FbaI TGATCA 3 cut(s) 2155, 2578, 2587
FblI GTMKAC 3 cut(s) 231, 1059, 1566
FokI GGATG 4 cut(s) 641, 889, 1097, 1406
FriOI GRGCYC 2 cut(s) 753, 1796
FspBI CTAG 1 cut(s) 1308
GlaI GCGC 5 cut(s) 371, 579, 639, 1022, 1073
GsaI CCCAGC 1 cut(s) 54
GsuI CTGGAG 2 cut(s) 1199, 2193
HaeII RGCGCY 2 cut(s) 641, 1024
HaeIII GGCC 9 cut(s) 404, 605, 939, 1013, 1151, 1202, 1483, 2485, 2651
HapII CCGG 2 cut(s) 189, 882
HgaI GACGC 2 cut(s) 281, 524
HhaI GCGC 5 cut(s) 372, 580, 640, 1023, 1074
Hin1I GRCGYC 1 cut(s) 516
Hin6I GCGC 5 cut(s) 370, 578, 638, 1021, 1072
HinP1I GCGC 5 cut(s) 370, 578, 638, 1021, 1072
HincII GTYRAC 1 cut(s) 1060
HindII GTYRAC 1 cut(s) 1060
HinfI GANTC 7 cut(s) 454, 1085, 1639, 1646, 1955, 2010, 2107
HpaII CCGG 2 cut(s) 189, 882
HphI GGTGA 5 cut(s) 106, 266, 799, 1727, 2573
Hpy99I CGWCG 5 cut(s) 275, 518, 617, 1009, 1061
HpyCH4III ACNGT 3 cut(s) 236, 931, 1555
HpyF3I CTNAG 4 cut(s) 623, 2051, 2432, 2544
Hsp92I GRCGYC 1 cut(s) 516
HspAI GCGC 5 cut(s) 370, 578, 638, 1021, 1072
Ksp22I TGATCA 3 cut(s) 2155, 2578, 2587
KspI CCGCGG 3 cut(s) 253, 603, 955
Kzo9I GATC 7 cut(s) 345, 553, 2155, 2280, 2392, 2578, 2587
LguI GCTCTTC 1 cut(s) 1579
LmnI GCTCC 7 cut(s) 188, 316, 1334, 1609, 1799, 2147, 2212
Lsp1109I GCAGC 4 cut(s) 354, 493, 1010, 1257
LweI GCATC 3 cut(s) 311, 911, 2461
MabI ACCWGGT 2 cut(s) 465, 1170
MaeI CTAG 1 cut(s) 1308
MaeIII GTNAC 6 cut(s) 442, 875, 1144, 1549, 2081, 2198
MalI GATC 7 cut(s) 347, 555, 2157, 2282, 2394, 2580, 2589
MbiI CCGCTC 1 cut(s) 183
MboI GATC 7 cut(s) 345, 553, 2155, 2280, 2392, 2578, 2587
MboII GAAGA 9 cut(s) 145, 232, 900, 934, 1319, 1503, 1596, 1789, 2480
MfeI CAATTG 1 cut(s) 2611
MflI RGATCY 1 cut(s) 2392
MhlI GDGCHC 6 cut(s) 587, 753, 1292, 1796, 2332, 2608
MluCI AATT 9 cut(s) 545, 892, 1655, 1814, 2022, 2181, 2357, 2426, 2611
MluI ACGCGT 1 cut(s) 872
MlyI GAGTC 4 cut(s) 448, 1094, 1633, 1655
MmeI TCCRAC 4 cut(s) 195, 855, 987, 2361
MseI TTAA 4 cut(s) 1118, 1740, 1818, 2072
MspA1I CMGCKG 4 cut(s) 252, 602, 954, 1975
MspI CCGG 2 cut(s) 189, 882
MspR9I CCNGG 4 cut(s) 414, 467, 776, 1172
MunI CAATTG 1 cut(s) 2611
MvaI CCWGG 4 cut(s) 414, 467, 776, 1172
MvnI CGCG 6 cut(s) 252, 530, 578, 602, 874, 954
NdeII GATC 7 cut(s) 345, 553, 2155, 2280, 2392, 2578, 2587
NlaIV GGNNCC 9 cut(s) 268, 312, 1261, 1336, 1669, 2149, 2191, 2208, 2394
NmeAIII GCCGAG 2 cut(s) 380, 430
NruI TCGCGA 1 cut(s) 530
NspI RCATGY 2 cut(s) 367, 1069
PaeI GCATGC 1 cut(s) 367
PaeR7I CTCGAG 1 cut(s) 1016
PceI AGGCCT 1 cut(s) 1483
PciSI GCTCTTC 1 cut(s) 1579
PcsI WCGNNNNNNNCGW 1 cut(s) 1626
PfeI GAWTC 3 cut(s) 1955, 2010, 2107
Pfl23II CGTACG 1 cut(s) 378
PflFI GACNNNGTC 2 cut(s) 962, 2491
PflMI CCANNNNNTGG 2 cut(s) 472, 1177
PleI GAGTC 4 cut(s) 448, 1093, 1633, 1654
PpsI GAGTC 4 cut(s) 448, 1093, 1633, 1654
Ppu21I YACGTR 2 cut(s) 91, 382
PpuMI RGGWCCY 1 cut(s) 592
PsiI TTATAA 1 cut(s) 1046
Psp124BI GAGCTC 1 cut(s) 1796
Psp1406I AACGTT 1 cut(s) 1620
Psp5II RGGWCCY 1 cut(s) 592
Psp6I CCWGG 4 cut(s) 412, 465, 774, 1170
PspFI CCCAGC 1 cut(s) 50
PspGI CCWGG 4 cut(s) 412, 465, 774, 1170
PspLI CGTACG 1 cut(s) 378
PspN4I GGNNCC 9 cut(s) 268, 312, 1261, 1336, 1669, 2149, 2191, 2208, 2394
PspPI GGNCC 4 cut(s) 592, 1012, 1260, 2650
PspPPI RGGWCCY 1 cut(s) 592
PspXI VCTCGAGB 1 cut(s) 1016
PstNI CAGNNNCTG 3 cut(s) 472, 595, 1177
PsuI RGATCY 1 cut(s) 2392
PsyI GACNNNGTC 2 cut(s) 962, 2491
PvuII CAGCTG 1 cut(s) 1975
RruI TCGCGA 1 cut(s) 530
SacI GAGCTC 1 cut(s) 1796
SacII CCGCGG 3 cut(s) 253, 603, 955
SalI GTCGAC 1 cut(s) 1058
SapI GCTCTTC 1 cut(s) 1579
SaqAI TTAA 4 cut(s) 1118, 1740, 1818, 2072
Sau3AI GATC 7 cut(s) 345, 553, 2155, 2280, 2392, 2578, 2587
Sau96I GGNCC 4 cut(s) 592, 1012, 1260, 2650
ScaI AGTACT 2 cut(s) 785, 2268
SchI GAGTC 4 cut(s) 448, 1094, 1633, 1655
ScrFI CCNGG 4 cut(s) 414, 467, 776, 1172
SduI GDGCHC 6 cut(s) 587, 753, 1292, 1796, 2332, 2608
SexAI ACCWGGT 2 cut(s) 465, 1170
SfaNI GCATC 3 cut(s) 311, 911, 2461
Sfr274I CTCGAG 1 cut(s) 1016
Sfr303I CCGCGG 3 cut(s) 253, 603, 955
SgrBI CCGCGG 3 cut(s) 253, 603, 955
SinI GGWCC 2 cut(s) 592, 1260
SlaI CTCGAG 1 cut(s) 1016
SmlI CTYRAG 5 cut(s) 1016, 1643, 2016, 2163, 2276
SmoI CTYRAG 5 cut(s) 1016, 1643, 2016, 2163, 2276
SphI GCATGC 1 cut(s) 367
Sse9I AATT 9 cut(s) 545, 892, 1655, 1814, 2022, 2181, 2357, 2426, 2611
SseBI AGGCCT 1 cut(s) 1483
SspI AATATT 1 cut(s) 2042
SspMI CTAG 1 cut(s) 1308
SstI GAGCTC 1 cut(s) 1796
StuI AGGCCT 1 cut(s) 1483
StyD4I CCNGG 4 cut(s) 412, 465, 774, 1170
TaaI ACNGT 3 cut(s) 236, 931, 1555
TaqI TCGA 4 cut(s) 330, 1017, 1059, 1659
TaqII GACCGA 1 cut(s) 2608
TasI AATT 9 cut(s) 545, 892, 1655, 1814, 2022, 2181, 2357, 2426, 2611
TatI WGTACW 5 cut(s) 567, 783, 1122, 1807, 2266
TauI GCSGC 6 cut(s) 252, 479, 605, 954, 957, 1164
TfiI GAWTC 3 cut(s) 1955, 2010, 2107
Tru1I TTAA 4 cut(s) 1118, 1740, 1818, 2072
Tru9I TTAA 4 cut(s) 1118, 1740, 1818, 2072
TscAI CASTG 6 cut(s) 556, 587, 934, 976, 1292, 1779
TseI GCWGC 4 cut(s) 367, 506, 1023, 1270
TspGWI ACGGA 5 cut(s) 7, 83, 196, 461, 2385
TspRI CASTG 6 cut(s) 556, 587, 934, 976, 1292, 1779
Tth111I GACNNNGTC 2 cut(s) 962, 2491
Van91I CCANNNNNTGG 2 cut(s) 472, 1177
VneI GTGCAC 3 cut(s) 583, 1288, 2328
VpaK11BI GGWCC 2 cut(s) 592, 1260
XagI CCTNNNNNAGG 1 cut(s) 1471
XapI RAATTY 3 cut(s) 545, 1814, 2426
XceI RCATGY 2 cut(s) 367, 1069
XhoI CTCGAG 1 cut(s) 1016
XmiI GTMKAC 3 cut(s) 231, 1059, 1566
XspI CTAG 1 cut(s) 1308
ZrmI AGTACT 2 cut(s) 785, 2268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.