Rw5G003640

Cysteine-rich receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000283
Physical Location & Seq
Forward (+)
3535117 .. 3538153
3037 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G003640.1

Sequence Viewer

Length: 1980 bp
ATGGCAGAAGTGATCCTTCTCTTCCTTTCAGTGGTCACCCTGGTAAGCTTTCCGACTACAAAAGCCGACTACCTCTACCATAGCTGCCCAAACACCACCGTCTTCACCCCAAACTCCACCTTCCAGTCCAACCTCAACCACCTCTTCTCTACCCTCTCCTCCAACGCCACACGCGACACTGGCTTCTACAACGCCACCTCCGGCCGTACCCCTAATGAAACCGTCTACGGCCTCTTCCTTTGCCGCGGTGACGTCACCACAGCCGCTTGCCAGTCCTGCGTGACCACCGCAACCTCCGAGGTCGTGCACCGCTGCCCGGTTGAGAAAGAGGTTGTGATTTGGTACGACGACTGCTTGCTCCGCTACTCCAACAAGTCCTTCTTCTCCGTCGCCACTGAGTCGCCGGGGGTGTTCATGTGGAACACGGGTAACATAAGCAGCGAACCTAACCGGTTCAACCAGGTTTTGGCGGCGAGCATGAAGGAGGTGGCCACGGCGGCTTCGAACGGCGGCGACAAGTTCGCGACGAGAGAGGCGGATTTCACGGAATTGATTAGTTTGTACAGCTTAGGGCAGTGCACGCAGGACTTGTCTAGATTTGATTGCAATCGGTGTCTTCTGGGAGCCATATCGCAGTTGCCGGGTTGCTGCGGCGGGAAGCAAGGGGGACGAGTTCTGTATCCGAGTTGTAATGTTAGGTATGAAGTTTATCCCTTTTATAACTCGACGTCAGCACCGGCACCGGCGCCGGCGCCAGGGGCTCAACCTCTTTCTCCGCCGAAGGGGAAGAGCAAAATCTCCTGGAAAAAGATTGTGGCCATTGCCGTTCCAATTGCTGCCTCTTTGGTGCTTTTTGTGATCGGCTCCTGCCTCGTTACTAAGAGGGCAAGAAAGAAGTACAATGCAGTACCAGAAACAAGTGGTGCGAATGACATTTCTAGTGTAGAGTCTTTGCAATTTGATTTTGGTACCATTGAAGCTGCTACAAACAATTTCTCTGACGATAACAAGTTAGGCGAAGGTGGATTTGGTCAAGTCTACAAGGGTTTGCTTTCTGATGGTCAAGAAATAGCTGTCAAGAGGCTATCAAGGAACTCTGGCCAAGGTGCACAAGAATTTAAGAATGAGATGGTACTGGTAGCCAAGCTTCAACACCGAAATCTGGTCAGGCTATTGGGATTTTGCTTGGAAGGGGAAGAAAAGATGCTTGTTTATGAATACGTCCCCAACAAAAGCCTCGACTATATTCTATTTGACCCTGAGAAACAAGGACAACTGGATTGGTCGAGACGTTACAAAATTATTGCGGGGATTGCCCGAGGAATTATGTATCTACATGAAGACTCCCGGCTAAGAATTATACATCGCGATCTTAAAGCCAGCAATATATTGTTAGATGGGGAAATGCATCCAAAAATATCTGATTTTGGCATGGCGAGGATATTCGGCGTTGATCAAACTCAAGCAAACACAAATAGAATTGTTGGAACATATGGTTACATGTCTCCAGAATATGCAATGCATGGACAATTTTCTATCAAGTCCGATATCTATAGTTTTGGTGTCTTAGTTCTAGAAATTATCAGTGGCAAGAAGAATAGTTACTTCTTCCAAACAGATGCAGCTGAGGACCTCGTGAGCCATGCTTGGAAACTATGGAGAGATGGAACACCTTTGGAATTGTTGGATCCGTGTCTGAGAGATTCTTATTCTAGAAATGAAGTCGTCAGATGTATCCACATTGGCTTGCTTTGTGTTCAGGAAGACCCGGCTGACAGACCTACAATGCAATCACTAGTTCTCATGCTCAACAGCTACTCTGTTACTCTACCATTACCTCAAAAGCCAGCATTTTTCCATAGGACTGGATCGGAGTTCATGCCGAAGATACCGCTGGATTCTGATCAGTCTACCAGCAAGTCCAGCAAGTCAAGTCCGTCGGTTTATGATGGATCTATCACTGAAGTATATCCTCGATAA

Protein Analysis

659

Amino Acids

72.89

Weight (kDa)

6.83

Isoelectric Point (pI)

46.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stress-antifung PF01657 29 - 125 2.6e-28 Salt stress response/antifungal
Stress-antifung PF01657 147 - 236 1.6e-10 Salt stress response/antifungal
Pkinase PF00069 333 - 600 5.6e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 335 - 601 1.2e-48 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000283)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G45860 AT4G05200 AT4G05200 AT4G11521 AT4G11530 AT4G23130 AT4G23130 AT4G23130 AT4G23140 AT4G23140 AT4G23150 AT4G23160 AT4G23170 AT4G23180 AT4G23180 AT4G23180 AT4G23230 AT4G23270 AT4G23270 AT4G23270 AT4G23280 AT4G23280 AT4G23280 AT4G23310 AT4G23310
fragaria_vesca FvH4_3g02772 FvH4_3g02772 FvH4_3g02773 FvH4_3g02773 FvH4_3g02774 FvH4_3g02800 FvH4_3g02820 FvH4_3g02820 FvH4_3g02830
malus_domestica MD00G1101900.v1.1 MD00G1102100.v1.1 MD00G1102200.v1.1 MD01G1098200.v1.1 MD05G1340000.v1.1 MD05G1340300.v1.1 MD07G1014100.v1.1 MD07G1014300.v1.1 MD07G1014400.v1.1 MD07G1014700.v1.1 MD10G1312900.v1.1 MD11G1022600.v1.1 MD11G1291500.v1.1 MD14G1207100.v1.1 MD16G1096600.v1.1
prunus_persica Prupe.4G027300_v2.0.a1 Prupe.4G027400_v2.0.a1 Prupe.4G027700_v2.0.a1 Prupe.4G027800_v2.0.a1
pyrus_communis pycom05g30960 pycom10g00940 pycom10g00950 pycom10g26430 pycom10g26450 pycom10g26460 pycom10g26470 pycom10g26480 pycom10g26490 pycom10g26500 pycom10g26510 pycom10g26570 pycom11g01820 pycom11g04000 pycom14g17130 pycom14g17140 pycom15g28820
rosa_chinensis RchiOBHm_Chr4g0408141 RchiOBHm_Chr5g0004211 RchiOBHm_Chr5g0004231 RchiOBHm_Chr5g0004241 RchiOBHm_Chr5g0004261
rosa_laevigata RLG00000008668 RLG00000008672 RLG00000031212 RLG00000031217
rosa_multiflora Rmu_co8162658.1_g000001 Rmu_co8336297.1_g000001 Rmu_sc0002073.1_g000001 Rmu_sc0004964.1_g000002 Rmu_sc0004964.1_g000003 Rmu_sc0004964.1_g000007 Rmu_sc0006006.1_g000007 Rmu_sc0007868.1_g000015 Rmu_sc0007868.1_g000016 Rmu_sc0007868.1_g000017 Rmu_sc0007868.1_g000021 Rmu_sc0007868.1_g000023 Rmu_sc0019654.1_g000002 Rmu_sc0038540.1_g000001
rosa_roxburghii Rroxscaffold_1G00071450 Rroxscaffold_1G00071460 Rroxscaffold_1G00071470 Rroxscaffold_1G00071560 Rroxscaffold_1G00071720 Rroxscaffold_1G00071770 Rroxscaffold_5G00352640
rosa_rugosa Rorug04G0083700 Rorug04G0410700 Rorug04G0410800 Rorug04G0410900
rosa_samantha Rh4BG145400 Rh4CG154900 Rh4DG141900 Rh5BG037500 Rh5BG037600 Rh5BG037700 Rh5CG041000 Rh5CG041100 Rh5CG041200 Rh5CG041300 Rh5CG041400 Rh5DG037100
rosa_wichuraiana Rw4G012130 Rw5G003450 Rw5G003460 Rw5G003470 Rw5G003480 Rw5G003640 Rw5G003660 Rw5G003670 Rw5G003680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 720
AatII GACGTC 2 cut(s) 255, 731
Acc65I GGTACC 1 cut(s) 968
AccB1I GGYRCC 4 cut(s) 739, 745, 751, 968
AccB7I CCANNNNNTGG 1 cut(s) 466
AccI GTMKAC 3 cut(s) 225, 1038, 1910
AccII CGCG 4 cut(s) 174, 246, 524, 1368
AclWI GGATC 5 cut(s) 7, 1682, 1695, 1876, 1960
AcoI YGGCCR 4 cut(s) 202, 489, 816, 1099
AcsI RAATTY 1 cut(s) 1115
AcyI GRCGYC 4 cut(s) 252, 728, 746, 752
AfaI GTAC 7 cut(s) 208, 344, 563, 899, 909, 970, 1134
AfiI CCNNNNNNNGG 6 cut(s) 31, 316, 466, 755, 782, 1162
AflIII ACRYGT 1 cut(s) 1500
AgeI ACCGGT 1 cut(s) 450
AgsI TTSAA 3 cut(s) 457, 977, 1151
AhlI ACTAGT 1 cut(s) 1795
AjnI CCWGG 4 cut(s) 39, 459, 754, 800
AjuI GAANNNNNNNTTGG 4 cut(s) 362, 394, 1011, 1043
AluBI AGCT 8 cut(s) 48, 84, 567, 980, 1073, 1147, 1625, 1815
AluI AGCT 8 cut(s) 48, 84, 567, 980, 1073, 1147, 1625, 1815
Alw21I GWGCWC 3 cut(s) 309, 581, 1111
Alw26I GTCTC 2 cut(s) 1282, 1509
Alw44I GTGCAC 3 cut(s) 305, 577, 1107
AlwI GGATC 5 cut(s) 7, 1682, 1695, 1876, 1960
Ama87I CYCGRG 1 cut(s) 1317
AoxI GGCC 5 cut(s) 202, 229, 489, 816, 1099
ApaLI GTGCAC 3 cut(s) 305, 577, 1107
ApeKI GCWGC 7 cut(s) 84, 312, 438, 648, 836, 980, 1622
ApoI RAATTY 1 cut(s) 1115
AsiGI ACCGGT 1 cut(s) 450
Asp718I GGTACC 1 cut(s) 968
AspLEI GCGC 2 cut(s) 748, 754
AspS9I GGNCC 1 cut(s) 1630
AsuC2I CCSGG 5 cut(s) 317, 405, 642, 1348, 1769
AsuHPI GGTGA 4 cut(s) 28, 97, 247, 260
AsuII TTCGAA 1 cut(s) 503
AvaI CYCGRG 1 cut(s) 1317
AvaII GGWCC 1 cut(s) 1630
BaeGI GKGCMC 3 cut(s) 309, 581, 1111
BalI TGGCCA 3 cut(s) 491, 818, 1101
BamHI GGATCC 1 cut(s) 1687
BanI GGYRCC 4 cut(s) 739, 745, 751, 968
BanII GRGCYC 1 cut(s) 763
BarI GAAGNNNNNNTAC 4 cut(s) 1131, 1163, 1586, 1618
BauI CACGAG 1 cut(s) 1634
BbsI GAAGAC 4 cut(s) 94, 608, 1347, 1770
Bbv12I GWGCWC 3 cut(s) 309, 581, 1111
BbvCI CCTCAGC 1 cut(s) 1626
BbvI GCAGC 7 cut(s) 71, 299, 450, 635, 823, 967, 1634
BccI CCATC 5 cut(s) 1052, 1123, 1391, 1658, 1943
BceAI ACGGC 5 cut(s) 189, 244, 510, 523, 809
BciT130I CCWGG 4 cut(s) 41, 461, 756, 802
BciVI GTATCC 2 cut(s) 690, 1745
BclI TGATCA 2 cut(s) 1453, 1903
BcnI CCSGG 5 cut(s) 317, 405, 642, 1348, 1769
BcoDI GTCTC 2 cut(s) 1282, 1509
BcuI ACTAGT 1 cut(s) 1795
BfaI CTAG 5 cut(s) 594, 939, 1574, 1713, 1796
BfmI CTRYAG 1 cut(s) 1552
BfoI RGCGCY 2 cut(s) 749, 755
BfuI GTATCC 2 cut(s) 690, 1745
BglI GCCNNNNNGGC 1 cut(s) 497
Bme1390I CCNGG 9 cut(s) 41, 317, 405, 461, 642, 756, 802, 1348, 1769
Bme18I GGWCC 1 cut(s) 1630
BmeT110I CYCGRG 1 cut(s) 1317
BmgT120I GGNCC 1 cut(s) 1630
BmiI GGNNCC 7 cut(s) 625, 741, 747, 753, 865, 970, 1689
BmrFI CCNGG 9 cut(s) 41, 317, 405, 461, 642, 756, 802, 1348, 1769
BmsI GCATC 3 cut(s) 1194, 1417, 1609
BpiI GAAGAC 4 cut(s) 94, 608, 1347, 1770
BpmI CTGGAG 1 cut(s) 1491
Bpu10I CCTNAGC 2 cut(s) 568, 1626
Bpu14I TTCGAA 1 cut(s) 503
BpuEI CTTGAG 1 cut(s) 1446
BpuMI CCSGG 5 cut(s) 317, 405, 642, 1348, 1769
BsaBI GATNNNNATC 2 cut(s) 606, 1902
BsaHI GRCGYC 4 cut(s) 252, 728, 746, 752
BsaJI CCNNGG 8 cut(s) 39, 244, 297, 404, 492, 755, 1102, 1318
BsaWI WCCGGW 1 cut(s) 450
BsaXI ACNNNNNCTCC 2 cut(s) 182, 212
Bsc4I CCNNNNNNNGG 6 cut(s) 31, 316, 466, 755, 782, 1162
Bse118I RCCGGY 4 cut(s) 450, 736, 742, 748
Bse1I ACTGG 6 cut(s) 124, 184, 271, 1140, 1281, 1870
Bse3DI GCAATG 2 cut(s) 819, 1524
Bse8I GATNNNNATC 2 cut(s) 606, 1902
BseBI CCWGG 4 cut(s) 41, 461, 756, 802
BseDI CCNNGG 8 cut(s) 39, 244, 297, 404, 492, 755, 1102, 1318
BseGI GGATG 1 cut(s) 1408
BseJI GATNNNNATC 2 cut(s) 606, 1902
BseLI CCNNNNNNNGG 6 cut(s) 31, 316, 466, 755, 782, 1162
BseMI GCAATG 2 cut(s) 819, 1524
BseMII CTCAG 4 cut(s) 387, 1251, 1617, 1688
BseNI ACTGG 6 cut(s) 124, 184, 271, 1140, 1281, 1870
BseRI GAGGAG 1 cut(s) 148
BseSI GKGCMC 3 cut(s) 309, 581, 1111
BseX3I CGGCCG 1 cut(s) 202
BseXI GCAGC 7 cut(s) 71, 299, 450, 635, 823, 967, 1634
Bsh1236I CGCG 4 cut(s) 174, 246, 524, 1368
Bsh1285I CGRYCG 1 cut(s) 205
BshFI GGCC 5 cut(s) 204, 231, 491, 818, 1101
BshNI GGYRCC 4 cut(s) 739, 745, 751, 968
BshTI ACCGGT 1 cut(s) 450
BsiEI CGRYCG 1 cut(s) 205
BsiHKAI GWGCWC 3 cut(s) 309, 581, 1111
BsiHKCI CYCGRG 1 cut(s) 1317
BslFI GGGAC 2 cut(s) 681, 1208
BslI CCNNNNNNNGG 6 cut(s) 31, 316, 466, 755, 782, 1162
BsmAI GTCTC 2 cut(s) 1282, 1509
BsmBI CGTCTC 1 cut(s) 1282
BsmFI GGGAC 2 cut(s) 681, 1208
BsnI GGCC 5 cut(s) 204, 231, 491, 818, 1101
BsoBI CYCGRG 1 cut(s) 1317
Bsp119I TTCGAA 1 cut(s) 503
Bsp1286I GDGCHC 4 cut(s) 309, 581, 763, 1111
Bsp1407I TGTACA 1 cut(s) 561
Bsp143I GATC 8 cut(s) 12, 858, 1369, 1453, 1687, 1868, 1903, 1952
Bsp68I TCGCGA 2 cut(s) 524, 1368
BspANI GGCC 5 cut(s) 204, 231, 491, 818, 1101
BspCNI CTCAG 4 cut(s) 388, 1252, 1618, 1689
BspFNI CGCG 4 cut(s) 174, 246, 524, 1368
BspLI GGNNCC 7 cut(s) 625, 741, 747, 753, 865, 970, 1689
BspPI GGATC 5 cut(s) 7, 1682, 1695, 1876, 1960
BspQI GCTCTTC 1 cut(s) 782
BspT104I TTCGAA 1 cut(s) 503
BspT107I GGYRCC 4 cut(s) 739, 745, 751, 968
BsrDI GCAATG 2 cut(s) 819, 1524
BsrFI RCCGGY 4 cut(s) 450, 736, 742, 748
BsrGI TGTACA 1 cut(s) 561
BsrI ACTGG 6 cut(s) 124, 184, 271, 1140, 1281, 1870
BssAI RCCGGY 4 cut(s) 450, 736, 742, 748
BssECI CCNNGG 8 cut(s) 39, 244, 297, 404, 492, 755, 1102, 1318
BssMI GATC 8 cut(s) 12, 858, 1369, 1453, 1687, 1868, 1903, 1952
BssNI GRCGYC 4 cut(s) 252, 728, 746, 752
BssSI CACGAG 1 cut(s) 1634
BssT1I CCWWGG 1 cut(s) 1102
Bst2BI CACGAG 1 cut(s) 1634
Bst2UI CCWGG 4 cut(s) 41, 461, 756, 802
Bst4CI ACNGT 2 cut(s) 100, 223
Bst6I CTCTTC 4 cut(s) 26, 149, 239, 782
BstACI GRCGYC 4 cut(s) 252, 728, 746, 752
BstAUI TGTACA 1 cut(s) 561
BstBI TTCGAA 1 cut(s) 503
BstC8I GCNNGC 8 cut(s) 268, 356, 475, 581, 750, 1381, 1748, 1848
BstDEI CTNAG 8 cut(s) 396, 568, 879, 1260, 1352, 1567, 1626, 1697
BstDSI CCRYGG 2 cut(s) 244, 492
BstEII GGTNACC 1 cut(s) 34
BstF5I GGATG 1 cut(s) 1408
BstFNI CGCG 4 cut(s) 174, 246, 524, 1368
BstH2I RGCGCY 2 cut(s) 749, 755
BstHHI GCGC 2 cut(s) 748, 754
BstKTI GATC 8 cut(s) 15, 861, 1372, 1456, 1690, 1871, 1906, 1955
BstMAI GTCTC 2 cut(s) 1282, 1509
BstMBI GATC 8 cut(s) 12, 858, 1369, 1453, 1687, 1868, 1903, 1952
BstMCI CGRYCG 1 cut(s) 205
BstMWI GCNNNNNNNGC 8 cut(s) 180, 276, 360, 497, 580, 758, 1313, 1923
BstNI CCWGG 4 cut(s) 41, 461, 756, 802
BstNSI RCATGY 1 cut(s) 1504
BstPI GGTNACC 1 cut(s) 34
BstSCI CCNGG 9 cut(s) 39, 315, 403, 459, 640, 754, 800, 1346, 1767
BstSFI CTRYAG 1 cut(s) 1552
BstSLI GKGCMC 3 cut(s) 309, 581, 1111
BstUI CGCG 4 cut(s) 174, 246, 524, 1368
BstV1I GCAGC 7 cut(s) 71, 299, 450, 635, 823, 967, 1634
BstV2I GAAGAC 4 cut(s) 94, 608, 1347, 1770
BstX2I RGATCY 2 cut(s) 1687, 1952
BstXI CCANNNNNNTGG 1 cut(s) 1865
BstYI RGATCY 2 cut(s) 1687, 1952
BstZI CGGCCG 1 cut(s) 202
BsuI GTATCC 2 cut(s) 690, 1745
BsuRI GGCC 5 cut(s) 204, 231, 491, 818, 1101
BtgI CCRYGG 2 cut(s) 244, 492
BtgZI GCGATG 1 cut(s) 1349
BtsCI GGATG 1 cut(s) 1408
BtsI GCAGTG 1 cut(s) 581
BtsIMutI CAGTG 6 cut(s) 36, 177, 393, 581, 1591, 1959
BtuMI TCGCGA 2 cut(s) 524, 1368
Cac8I GCNNGC 8 cut(s) 268, 356, 475, 581, 750, 1381, 1748, 1848
CfoI GCGC 2 cut(s) 748, 754
Cfr10I RCCGGY 4 cut(s) 450, 736, 742, 748
Cfr13I GGNCC 1 cut(s) 1630
Cfr42I CCGCGG 1 cut(s) 247
CsiI ACCWGGT 1 cut(s) 459
Csp6I GTAC 7 cut(s) 207, 343, 562, 898, 908, 969, 1133
CspAI ACCGGT 1 cut(s) 450
CviAII CATG 9 cut(s) 415, 478, 1337, 1432, 1501, 1523, 1643, 1804, 1879
CviQI GTAC 7 cut(s) 207, 343, 562, 898, 908, 969, 1133
DdeI CTNAG 8 cut(s) 396, 568, 879, 1260, 1352, 1567, 1626, 1697
DinI GGCGCC 2 cut(s) 747, 753
DpnI GATC 8 cut(s) 14, 860, 1371, 1455, 1689, 1870, 1905, 1954
DpnII GATC 8 cut(s) 12, 858, 1369, 1453, 1687, 1868, 1903, 1952
EaeI YGGCCR 4 cut(s) 202, 489, 816, 1099
EagI CGGCCG 1 cut(s) 202
Eam1104I CTCTTC 4 cut(s) 26, 149, 239, 782
EarI CTCTTC 4 cut(s) 26, 149, 239, 782
EciI GGCGGA 2 cut(s) 551, 765
EclXI CGGCCG 1 cut(s) 202
Eco130I CCWWGG 1 cut(s) 1102
Eco24I GRGCYC 1 cut(s) 763
Eco32I GATATC 1 cut(s) 1549
Eco47I GGWCC 1 cut(s) 1630
Eco52I CGGCCG 1 cut(s) 202
Eco88I CYCGRG 1 cut(s) 1317
Eco91I GGTNACC 1 cut(s) 34
EcoO109I RGGNCCY 1 cut(s) 1630
EcoO65I GGTNACC 1 cut(s) 34
EcoRII CCWGG 4 cut(s) 39, 459, 754, 800
EcoRV GATATC 1 cut(s) 1549
EcoT14I CCWWGG 1 cut(s) 1102
EcoT22I ATGCAT 2 cut(s) 1410, 1524
EcoT38I GRGCYC 1 cut(s) 763
EgeI GGCGCC 2 cut(s) 747, 753
EheI GGCGCC 2 cut(s) 747, 753
ErhI CCWWGG 1 cut(s) 1102
Esp3I CGTCTC 1 cut(s) 1282
FaeI CATG 9 cut(s) 418, 481, 1340, 1435, 1504, 1526, 1646, 1807, 1882
FalI AAGNNNNNCTT 3 cut(s) 32, 365, 397
FaqI GGGAC 2 cut(s) 681, 1208
FatI CATG 9 cut(s) 414, 477, 1336, 1431, 1500, 1522, 1642, 1803, 1878
FauI CCCGC 2 cut(s) 647, 1300
FauNDI CATATG 1 cut(s) 1492
FbaI TGATCA 2 cut(s) 1453, 1903
FblI GTMKAC 3 cut(s) 225, 1038, 1910
FokI GGATG 1 cut(s) 1395
FriOI GRGCYC 1 cut(s) 763
FspBI CTAG 5 cut(s) 594, 939, 1574, 1713, 1796
GlaI GCGC 2 cut(s) 747, 753
GsuI CTGGAG 1 cut(s) 1491
HaeII RGCGCY 2 cut(s) 749, 755
HaeIII GGCC 5 cut(s) 204, 231, 491, 818, 1101
HhaI GCGC 2 cut(s) 748, 754
Hin1I GRCGYC 4 cut(s) 252, 728, 746, 752
Hin1II CATG 9 cut(s) 418, 481, 1340, 1435, 1504, 1526, 1646, 1807, 1882
Hin6I GCGC 2 cut(s) 746, 752
HinP1I GCGC 2 cut(s) 746, 752
HindIII AAGCTT 2 cut(s) 46, 1145
HinfI GANTC 5 cut(s) 398, 947, 1343, 1703, 1898
HphI GGTGA 4 cut(s) 28, 97, 247, 260
Hpy166II GTNNAC 6 cut(s) 226, 307, 579, 1039, 1109, 1911
Hpy8I GTNNAC 6 cut(s) 226, 307, 579, 1039, 1109, 1911
Hpy99I CGWCG 5 cut(s) 350, 392, 529, 730, 1942
HpyAV CCTTC 7 cut(s) 26, 130, 388, 475, 775, 1013, 1184
HpyCH4III ACNGT 2 cut(s) 100, 223
HpyCH4IV ACGT 4 cut(s) 252, 728, 1221, 1291
HpyF10VI GCNNNNNNNGC 8 cut(s) 180, 276, 360, 497, 580, 758, 1313, 1923
HpyF3I CTNAG 8 cut(s) 396, 568, 879, 1260, 1352, 1567, 1626, 1697
HpySE526I ACGT 4 cut(s) 252, 728, 1221, 1291
Hsp92I GRCGYC 4 cut(s) 252, 728, 746, 752
Hsp92II CATG 9 cut(s) 418, 481, 1340, 1435, 1504, 1526, 1646, 1807, 1882
HspAI GCGC 2 cut(s) 746, 752
KasI GGCGCC 2 cut(s) 745, 751
KpnI GGTACC 1 cut(s) 972
KroI GCCGGC 1 cut(s) 748
KroNI GCCGGC 1 cut(s) 750
Ksp22I TGATCA 2 cut(s) 1453, 1903
KspI CCGCGG 1 cut(s) 247
Kzo9I GATC 8 cut(s) 12, 858, 1369, 1453, 1687, 1868, 1903, 1952
LguI GCTCTTC 1 cut(s) 782
LmnI GCTCC 3 cut(s) 363, 623, 869
Lsp1109I GCAGC 7 cut(s) 71, 299, 450, 635, 823, 967, 1634
LweI GCATC 3 cut(s) 1194, 1417, 1609
MabI ACCWGGT 1 cut(s) 459
MaeI CTAG 5 cut(s) 594, 939, 1574, 1713, 1796
MaeII ACGT 4 cut(s) 252, 728, 1221, 1291
MalI GATC 8 cut(s) 14, 860, 1371, 1455, 1689, 1870, 1905, 1954
MboI GATC 8 cut(s) 12, 858, 1369, 1453, 1687, 1868, 1903, 1952
MfeI CAATTG 1 cut(s) 831
MflI RGATCY 2 cut(s) 1687, 1952
MhlI GDGCHC 4 cut(s) 309, 581, 763, 1111
MlsI TGGCCA 3 cut(s) 491, 818, 1101
MluNI TGGCCA 3 cut(s) 491, 818, 1101
Mly113I GGCGCC 2 cut(s) 746, 752
MlyI GAGTC 3 cut(s) 407, 956, 1337
MmeI TCCRAC 6 cut(s) 77, 153, 186, 393, 1465, 1665
Mox20I TGGCCA 3 cut(s) 491, 818, 1101
Mph1103I ATGCAT 2 cut(s) 1410, 1524
MreI CGCCGGCG 1 cut(s) 748
MroNI GCCGGC 1 cut(s) 748
MscI TGGCCA 3 cut(s) 491, 818, 1101
MseI TTAA 2 cut(s) 1119, 1374
Msp20I TGGCCA 3 cut(s) 491, 818, 1101
MspA1I CMGCKG 4 cut(s) 246, 312, 1625, 1894
MspR9I CCNGG 9 cut(s) 41, 317, 405, 461, 642, 756, 802, 1348, 1769
MunI CAATTG 1 cut(s) 831
MvaI CCWGG 4 cut(s) 41, 461, 756, 802
MvnI CGCG 4 cut(s) 174, 246, 524, 1368
MwoI GCNNNNNNNGC 8 cut(s) 180, 276, 360, 497, 580, 758, 1313, 1923
NaeI GCCGGC 1 cut(s) 750
NarI GGCGCC 2 cut(s) 746, 752
NciI CCSGG 5 cut(s) 317, 405, 642, 1348, 1769
NdeI CATATG 1 cut(s) 1492
NdeII GATC 8 cut(s) 12, 858, 1369, 1453, 1687, 1868, 1903, 1952
NgoMIV GCCGGC 1 cut(s) 748
NlaIII CATG 9 cut(s) 418, 481, 1340, 1435, 1504, 1526, 1646, 1807, 1882
NlaIV GGNNCC 7 cut(s) 625, 741, 747, 753, 865, 970, 1689
NmuCI GTSAC 4 cut(s) 34, 248, 253, 280
NruI TCGCGA 2 cut(s) 524, 1368
NsiI ATGCAT 2 cut(s) 1410, 1524
NspI RCATGY 1 cut(s) 1504
NspV TTCGAA 1 cut(s) 503
PciI ACATGT 1 cut(s) 1500
PciSI GCTCTTC 1 cut(s) 782
PcsI WCGNNNNNNNCGW 2 cut(s) 171, 500
PdiI GCCGGC 1 cut(s) 750
PfeI GAWTC 2 cut(s) 1703, 1898
PflMI CCANNNNNTGG 1 cut(s) 466
PfoI TCCNGGA 1 cut(s) 800
PinAI ACCGGT 1 cut(s) 450
PleI GAGTC 3 cut(s) 406, 955, 1337
PluTI GGCGCC 2 cut(s) 749, 755
PpsI GAGTC 3 cut(s) 406, 955, 1337
PpuMI RGGWCCY 1 cut(s) 1630
PscI ACATGT 1 cut(s) 1500
PsiI TTATAA 1 cut(s) 720
Psp5II RGGWCCY 1 cut(s) 1630
Psp6I CCWGG 4 cut(s) 39, 459, 754, 800
PspEI GGTNACC 1 cut(s) 34
PspGI CCWGG 4 cut(s) 39, 459, 754, 800
PspN4I GGNNCC 7 cut(s) 625, 741, 747, 753, 865, 970, 1689
PspPI GGNCC 1 cut(s) 1630
PspPPI RGGWCCY 1 cut(s) 1630
PsuI RGATCY 2 cut(s) 1687, 1952
PvuII CAGCTG 1 cut(s) 1625
RruI TCGCGA 2 cut(s) 524, 1368
RsaI GTAC 7 cut(s) 208, 344, 563, 899, 909, 970, 1134
RsaNI GTAC 7 cut(s) 207, 343, 562, 898, 908, 969, 1133
SacII CCGCGG 1 cut(s) 247
SapI GCTCTTC 1 cut(s) 782
SaqAI TTAA 2 cut(s) 1119, 1374
Sau3AI GATC 8 cut(s) 12, 858, 1369, 1453, 1687, 1868, 1903, 1952
Sau96I GGNCC 1 cut(s) 1630
SchI GAGTC 3 cut(s) 407, 956, 1337
ScrFI CCNGG 9 cut(s) 41, 317, 405, 461, 642, 756, 802, 1348, 1769
SduI GDGCHC 4 cut(s) 309, 581, 763, 1111
SexAI ACCWGGT 1 cut(s) 459
SfaNI GCATC 3 cut(s) 1194, 1417, 1609
SfcI CTRYAG 1 cut(s) 1552
SfoI GGCGCC 2 cut(s) 747, 753
Sfr303I CCGCGG 1 cut(s) 247
SfuI TTCGAA 1 cut(s) 503
SgrAI CRCCGGYG 2 cut(s) 742, 748
SgrBI CCGCGG 1 cut(s) 247
SinI GGWCC 1 cut(s) 1630
SmlI CTYRAG 1 cut(s) 1461
SmoI CTYRAG 1 cut(s) 1461
SpeI ACTAGT 1 cut(s) 1795
SspDI GGCGCC 2 cut(s) 745, 751
SspMI CTAG 5 cut(s) 594, 939, 1574, 1713, 1796
StyD4I CCNGG 9 cut(s) 39, 315, 403, 459, 640, 754, 800, 1346, 1767
StyI CCWWGG 1 cut(s) 1102
TaaI ACNGT 2 cut(s) 100, 223
TaiI ACGT 4 cut(s) 255, 731, 1224, 1294
TaqI TCGA 5 cut(s) 503, 725, 1239, 1286, 1975
TatI WGTACW 2 cut(s) 561, 897
TauI GCSGC 6 cut(s) 246, 266, 473, 500, 513, 654
TfiI GAWTC 2 cut(s) 1703, 1898
Tru1I TTAA 2 cut(s) 1119, 1374
Tru9I TTAA 2 cut(s) 1119, 1374
TscAI CASTG 6 cut(s) 36, 184, 400, 581, 1591, 1966
TseFI GTSAC 4 cut(s) 34, 248, 253, 280
TseI GCWGC 7 cut(s) 84, 312, 438, 648, 836, 980, 1622
Tsp45I GTSAC 4 cut(s) 34, 248, 253, 280
TspDTI ATGAA 8 cut(s) 231, 403, 494, 717, 1230, 1353, 1734, 1867
TspGWI ACGGA 4 cut(s) 376, 560, 1680, 1926
TspRI CASTG 6 cut(s) 36, 184, 400, 581, 1591, 1966
Van91I CCANNNNNTGG 1 cut(s) 466
VneI GTGCAC 3 cut(s) 305, 577, 1107
VpaK11BI GGWCC 1 cut(s) 1630
XapI RAATTY 1 cut(s) 1115
XbaI TCTAGA 3 cut(s) 593, 1573, 1712
XceI RCATGY 1 cut(s) 1504
XmiI GTMKAC 3 cut(s) 225, 1038, 1910
XspI CTAG 5 cut(s) 594, 939, 1574, 1713, 1796
ZraI GACGTC 2 cut(s) 253, 729
Zsp2I ATGCAT 2 cut(s) 1410, 1524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.