Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0000283

Physical Location & Seq

Genomic Coordinates Forward (+)

3257735 .. 3261375

Gene Structure 3641 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G003470.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2076 bp

ATGGATCAGAAACAGATCAACATAATGCCAAGTTGTTCTCATCATGTGTTCATGTCCTTTGTGTTGCTCTCCATTCTTGGTTTCCATAGCCTCAGCATCGAAGCTGAAGCTCAAGCTATAGCTGTAGTTGGTGGCTACTCCCGTTCCTGTTCAAACACAACAACTTTCACTCCGAACAGCACTTACCAGTTTAATCTCAATCTCCTCCTCTCCTATCTCACCTCCAACGCCACCAATGACCTCAGATTTTACAACACCACAGTCGGCGCCCAAGACCCCGGCACGACTGTTTACGGTTCATTTAGTTGCCGTGGCGATGTCACCCCTGAAATATGTCAACAATGTGTGTCTGCTATAGCCACAGAAGGAGTCCAAAATTATTGCCCCTTGAGCAAAATCTCAATAATATGGTATGCAGATTGCATGTTACGCTACTCAAACACATCCTTCTTTGGCAACCCGGAGACATCTCCTCAAGTTTCCTTCTTCAACACGGGGAATATCACTGAGCCTGACCGCTTCATGCCGCTGCTGGGACAAACTCTGAAGAGCTTGGTGGGACCTGCTGCTGGTGCACCTTCAGGTGTGAAAAAATTCGCAACAAAAGAAGTGAACTTCACAGATTTTCAGCAGTTGTACACCCTTGTGCAGTGCACACCAGACCTATCGAGCCTAGGTTGTGAAAGATGTCTTACAGCAGCTATTGCAGCTATTCCAGTTTCTTTTTATGGAAAGCAAGGGGGAGTAGTCTTATTTCCTACTGTTTATGTTCGATATGAAATCTATCCATTTTACACAGTTCAAGCTGCCACACCTCCACCAGCGCCATCGCCTCCATCACTGCTTCTTCCTCCCCCTCCTCCAGGTTCAGTAACGAGATCTCAAGGAAACGACAAAATCTCATCCTTGATCATAGTGGCCATTGTTGTTCCAATTGCCTTGGTGCTTTTCTTGGTAGGCTGCTTTTTTCTAAGAAGAAAGACAAGAAAAAAGTACGATGCTTTACAAGGCCAGAATGGGGAGAATGAAATTACTACTGTGGAGTCATTGCAGTTTGATTTTGCTACAATTGAAGCAGCCACAAACCAGTTCTCTGTTAATAATAGATTAGGGGAAGGTGGCTTTGGAGAGGTTTACAAGGGAACACTTCCTAATGGGCAAGAAATAGCTGTGAAGAGGTTATCTAGAAGCTCTGGGCAAGGTGTCATGGAATTTAAGAATGAGGTGGTTTTGGTAGCCAAGCTTCAGCACAGAAATTTAGTGAGGCTACTGGGATTTTGCTCTGAAGGAGAAGAAAAGATTCTTATCTATGAACTTGTGCAGAACAAAAGCCTTGACCACTTTTTATTCAACTCAGATATTCAAGTAAAGTTGGATTGGTCAAGCAGATACAAGATTATAGGGGGGATTGCTAGAGGGATTCTCTATCTCCATCAAGATTCTCGGCTTAAAATCATACATCGCGATCTCAAAGCAAGCAACATATTGTTAGATGGAGAAATGAACCCAAAAATATCAGATTTTGGAATGGCAAGAATATTTGGAGTTGATCAAACTCAAGGAAGCACCAGAAGGATTGTCGGAACTTATGGTTACATGTCCCCAGAGTATGCAATGCATGGACAGTTCTCAGAGAAGTCTGATGTGTACAGTCTTGGTGTCTTAATCTTAGAGATTGTAACAGGCAAGAAAAACACTAGTTTCTATCAATCAGACCGTGGCGAAGATCTCTTAACATATGCTTGGAAACATTGGAGAGAAGGGACGGTGGAAGAATTGTTGGATTCAAATCTGAGATGTTCTTATTCAAGAAATGAAGTAATCAGATGTATCCATATTGGTTTACTCTGTGTTCAGGAGGATCCAAAGGAGAGACCTACTATGCAAACCATTGTTCTCATGCTAAGCAGTTACTCAGTTTCTATGCCTTTGCCTGAGAAGCCAGCGTTTTTCCTGCATAGTAAGGCAGAGTTGAACATGCCATCAGTAGCCTCTGGATCATCATATCAACCCACTAGCAGCTCAGTCTCATGTTCTGTTAATGAAGTTTCAATTACTGAATTATTCCCTCGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0042742 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050896 GO:0051704 GO:0051707 GO:0071704 GO:0071944 GO:0098542 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase_Tyr Stress-antifung

Protein Analysis

691

Amino Acids

76.43

Weight (kDa)

6.62

Isoelectric Point (pI)

45.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Stress-antifung	PF01657	50 - 147	4.6e-23	Salt stress response/antifungal
Stress-antifung	PF01657	166 - 260	1.1e-06	Salt stress response/antifungal
Pkinase	PF00069	365 - 632	6.4e-46	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	366 - 633	2.1e-49	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0000283)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G45860 AT4G05200 AT4G05200 AT4G11521 AT4G11530 AT4G23130 AT4G23130 AT4G23130 AT4G23140 AT4G23140 AT4G23150 AT4G23160 AT4G23170 AT4G23180 AT4G23180 AT4G23180 AT4G23230 AT4G23270 AT4G23270 AT4G23270 AT4G23280 AT4G23280 AT4G23280 AT4G23310 AT4G23310
fragaria_vesca	FvH4_3g02772 FvH4_3g02772 FvH4_3g02773 FvH4_3g02773 FvH4_3g02774 FvH4_3g02800 FvH4_3g02820 FvH4_3g02820 FvH4_3g02830
malus_domestica	MD00G1101900.v1.1 MD00G1102100.v1.1 MD00G1102200.v1.1 MD01G1098200.v1.1 MD05G1340000.v1.1 MD05G1340300.v1.1 MD07G1014100.v1.1 MD07G1014300.v1.1 MD07G1014400.v1.1 MD07G1014700.v1.1 MD10G1312900.v1.1 MD11G1022600.v1.1 MD11G1291500.v1.1 MD14G1207100.v1.1 MD16G1096600.v1.1
prunus_persica	Prupe.4G027300_v2.0.a1 Prupe.4G027400_v2.0.a1 Prupe.4G027700_v2.0.a1 Prupe.4G027800_v2.0.a1
pyrus_communis	pycom05g30960 pycom10g00940 pycom10g00950 pycom10g26430 pycom10g26450 pycom10g26460 pycom10g26470 pycom10g26480 pycom10g26490 pycom10g26500 pycom10g26510 pycom10g26570 pycom11g01820 pycom11g04000 pycom14g17130 pycom14g17140 pycom15g28820
rosa_chinensis	RchiOBHm_Chr4g0408141 RchiOBHm_Chr5g0004211 RchiOBHm_Chr5g0004231 RchiOBHm_Chr5g0004241 RchiOBHm_Chr5g0004261
rosa_laevigata	RLG00000008668 RLG00000008672 RLG00000031212 RLG00000031217
rosa_multiflora	Rmu_co8162658.1_g000001 Rmu_co8336297.1_g000001 Rmu_sc0002073.1_g000001 Rmu_sc0004964.1_g000002 Rmu_sc0004964.1_g000003 Rmu_sc0004964.1_g000007 Rmu_sc0006006.1_g000007 Rmu_sc0007868.1_g000015 Rmu_sc0007868.1_g000016 Rmu_sc0007868.1_g000017 Rmu_sc0007868.1_g000021 Rmu_sc0007868.1_g000023 Rmu_sc0019654.1_g000002 Rmu_sc0038540.1_g000001
rosa_roxburghii	Rroxscaffold_1G00071450 Rroxscaffold_1G00071460 Rroxscaffold_1G00071470 Rroxscaffold_1G00071560 Rroxscaffold_1G00071720 Rroxscaffold_1G00071770 Rroxscaffold_5G00352640
rosa_rugosa	Rorug04G0083700 Rorug04G0410700 Rorug04G0410800 Rorug04G0410900
rosa_samantha	Rh4BG145400 Rh4CG154900 Rh4DG141900 Rh5BG037500 Rh5BG037600 Rh5BG037700 Rh5CG041000 Rh5CG041100 Rh5CG041200 Rh5CG041300 Rh5CG041400 Rh5DG037100
rosa_wichuraiana	Rw4G012130 Rw5G003450 Rw5G003460 Rw5G003470 Rw5G003480 Rw5G003640 Rw5G003660 Rw5G003670 Rw5G003680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	571
AccB1I	GGYRCC	1 cut(s)	266
AccII	CGCG	1 cut(s)	1464
AciI	CCGC	2 cut(s)	517, 527
AclWI	GGATC	4 cut(s)	12, 1856, 1869, 2005
AcoI	YGGCCR	1 cut(s)	918
AcsI	RAATTY	3 cut(s)	593, 1211, 1255
AcuI	CTGAAG	5 cut(s)	126, 564, 566, 1229, 1305
AcyI	GRCGYC	1 cut(s)	267
AfaI	GTAC	3 cut(s)	638, 995, 1649
AfiI	CCNNNNNNNGG	4 cut(s)	533, 569, 863, 1018
AflIII	ACRYGT	1 cut(s)	1596
AhlI	ACTAGT	1 cut(s)	1697
AjnI	CCWGG	1 cut(s)	862
AjuI	GAANNNNNNNTTGG	2 cut(s)	1107, 1139
AleI	CACNNNNGTG	1 cut(s)	644
Alw21I	GWGCWC	2 cut(s)	577, 656
Alw26I	GTCTC	3 cut(s)	458, 1867, 2032
Alw44I	GTGCAC	2 cut(s)	573, 652
AlwI	GGATC	4 cut(s)	12, 1856, 1869, 2005
AoxI	GGCC	2 cut(s)	918, 1009
ApaLI	GTGCAC	2 cut(s)	573, 652
ApeKI	GCWGC	8 cut(s)	529, 566, 698, 707, 806, 960, 1076, 2019
ApoI	RAATTY	3 cut(s)	593, 1211, 1255
Asp700I	GAANNNNTTC	2 cut(s)	593, 1299
AspA2I	CCTAGG	1 cut(s)	673
AspLEI	GCGC	2 cut(s)	269, 826
AspS9I	GGNCC	1 cut(s)	560
AsuC2I	CCSGG	2 cut(s)	279, 461
AsuHPI	GGTGA	2 cut(s)	211, 313
AvaII	GGWCC	1 cut(s)	560
AvrII	CCTAGG	1 cut(s)	673
BaeGI	GKGCMC	2 cut(s)	577, 656
BalI	TGGCCA	1 cut(s)	920
BamHI	GGATCC	1 cut(s)	1861
BanI	GGYRCC	1 cut(s)	266
BarI	GAAGNNNNNNTAC	2 cut(s)	1227, 1259
Bbv12I	GWGCWC	2 cut(s)	577, 656
BbvCI	CCTCAGC	1 cut(s)	92
BbvI	GCAGC	8 cut(s)	516, 553, 710, 719, 793, 947, 1088, 2031
BccI	CCATC	5 cut(s)	835, 844, 1440, 1487, 1990
BceAI	ACGGC	1 cut(s)	294
BciT130I	CCWGG	1 cut(s)	864
BciVI	GTATCC	1 cut(s)	1841
BclI	TGATCA	2 cut(s)	909, 1549
BcnI	CCSGG	2 cut(s)	279, 461
BcoDI	GTCTC	3 cut(s)	458, 1867, 2032
BcuI	ACTAGT	1 cut(s)	1697
BfaI	CTAG	5 cut(s)	674, 1185, 1413, 1698, 2016
BfmI	CTRYAG	3 cut(s)	117, 123, 354
BfoI	RGCGCY	2 cut(s)	270, 827
BfuAI	ACCTGC	1 cut(s)	571
BfuI	GTATCC	1 cut(s)	1841
BglII	AGATCT	2 cut(s)	878, 1726
BisI	GCNGC	9 cut(s)	527, 530, 567, 699, 708, 807, 961, 1077, 2020
BlnI	CCTAGG	1 cut(s)	673
BlpI	GCTNAGC	1 cut(s)	1904
BlsI	GCNGC	9 cut(s)	528, 531, 568, 700, 709, 808, 962, 1078, 2021
Bme1390I	CCNGG	3 cut(s)	279, 461, 864
Bme18I	GGWCC	1 cut(s)	560
BmgT120I	GGNCC	1 cut(s)	560
BmiI	GGNNCC	3 cut(s)	268, 561, 1863
BmrFI	CCNGG	3 cut(s)	279, 461, 864
BmrI	ACTGGG	1 cut(s)	1280
BmsI	GCATC	2 cut(s)	105, 988
BmuI	ACTGGG	1 cut(s)	1280
BplI	GAGNNNNNCTC	2 cut(s)	1407, 1439
BpmI	CTGGAG	1 cut(s)	846
Bpu10I	CCTNAGC	1 cut(s)	92
Bpu1102I	GCTNAGC	1 cut(s)	1904
BpuEI	CTTGAG	5 cut(s)	96, 409, 459, 867, 1542
BpuMI	CCSGG	2 cut(s)	279, 461
BsaBI	GATNNNNATC	2 cut(s)	1304, 1788
BsaHI	GRCGYC	1 cut(s)	267
BsaI	GGTCTC	1 cut(s)	1867
BsaJI	CCNNGG	5 cut(s)	277, 310, 673, 939, 1717
BsaXI	ACNNNNNCTCC	2 cut(s)	154, 184
Bsc4I	CCNNNNNNNGG	4 cut(s)	533, 569, 863, 1018
Bse1I	ACTGG	4 cut(s)	187, 716, 1087, 1275
Bse3DI	GCAATG	2 cut(s)	1046, 1620
Bse8I	GATNNNNATC	2 cut(s)	1304, 1788
BseBI	CCWGG	1 cut(s)	864
BseDI	CCNNGG	5 cut(s)	277, 310, 673, 939, 1717
BseGI	GGATG	2 cut(s)	443, 902
BseJI	GATNNNNATC	2 cut(s)	1304, 1788
BseLI	CCNNNNNNNGG	4 cut(s)	533, 569, 863, 1018
BseMI	GCAATG	2 cut(s)	1046, 1620
BseMII	CTCAG	9 cut(s)	106, 256, 498, 1368, 1644, 1784, 1926, 1929, 2037
BseNI	ACTGG	4 cut(s)	187, 716, 1087, 1275
BseRI	GAGGAG	4 cut(s)	194, 197, 462, 849
BseSI	GKGCMC	2 cut(s)	577, 656
BseXI	GCAGC	8 cut(s)	516, 553, 710, 719, 793, 947, 1088, 2031
BseYI	CCCAGC	1 cut(s)	532
BsgI	GTGCAG	2 cut(s)	668, 1340
Bsh1236I	CGCG	1 cut(s)	1464
BshFI	GGCC	2 cut(s)	920, 1011
BshNI	GGYRCC	1 cut(s)	266
BsiHKAI	GWGCWC	2 cut(s)	577, 656
BsiSI	CCGG	2 cut(s)	279, 461
BslFI	GGGAC	4 cut(s)	549, 573, 1585, 1777
BslI	CCNNNNNNNGG	4 cut(s)	533, 569, 863, 1018
BsmAI	GTCTC	3 cut(s)	458, 1867, 2032
BsmFI	GGGAC	4 cut(s)	549, 573, 1585, 1777
BsnI	GGCC	2 cut(s)	920, 1011
Bso31I	GGTCTC	1 cut(s)	1867
Bsp1286I	GDGCHC	2 cut(s)	577, 656
Bsp1407I	TGTACA	2 cut(s)	636, 1647
Bsp143I	GATC	9 cut(s)	4, 15, 878, 909, 1465, 1549, 1726, 1861, 1997
Bsp1720I	GCTNAGC	1 cut(s)	1904
Bsp68I	TCGCGA	1 cut(s)	1464
BspACI	CCGC	2 cut(s)	517, 527
BspANI	GGCC	2 cut(s)	920, 1011
BspCNI	CTCAG	9 cut(s)	105, 255, 499, 1367, 1643, 1785, 1927, 1928, 2036
BspFNI	CGCG	1 cut(s)	1464
BspLI	GGNNCC	3 cut(s)	268, 561, 1863
BspMI	ACCTGC	1 cut(s)	571
BspPI	GGATC	4 cut(s)	12, 1856, 1869, 2005
BspQI	GCTCTTC	1 cut(s)	542
BspT107I	GGYRCC	1 cut(s)	266
BspTNI	GGTCTC	1 cut(s)	1867
BsrDI	GCAATG	2 cut(s)	1046, 1620
BsrGI	TGTACA	2 cut(s)	636, 1647
BsrI	ACTGG	4 cut(s)	187, 716, 1087, 1275
BssECI	CCNNGG	5 cut(s)	277, 310, 673, 939, 1717
BssMI	GATC	9 cut(s)	4, 15, 878, 909, 1465, 1549, 1726, 1861, 1997
BssNI	GRCGYC	1 cut(s)	267
BssT1I	CCWWGG	2 cut(s)	673, 939
Bst2UI	CCWGG	1 cut(s)	864
Bst6I	CTCTTC	2 cut(s)	542, 1169
BstACI	GRCGYC	1 cut(s)	267
BstAPI	GCANNNNNTGC	1 cut(s)	704
BstAUI	TGTACA	2 cut(s)	636, 1647
BstC8I	GCNNGC	2 cut(s)	1477, 1944
BstDSI	CCRYGG	2 cut(s)	310, 1717
BstENI	CCTNNNNNAGG	1 cut(s)	861
BstF5I	GGATG	2 cut(s)	443, 902
BstFNI	CGCG	1 cut(s)	1464
BstH2I	RGCGCY	2 cut(s)	270, 827
BstHHI	GCGC	2 cut(s)	269, 826
BstKTI	GATC	9 cut(s)	7, 18, 881, 912, 1468, 1552, 1729, 1864, 2000
BstMAI	GTCTC	3 cut(s)	458, 1867, 2032
BstMBI	GATC	9 cut(s)	4, 15, 878, 909, 1465, 1549, 1726, 1861, 1997
BstMWI	GCNNNNNNNGC	6 cut(s)	390, 429, 572, 704, 707, 1939
BstNI	CCWGG	1 cut(s)	864
BstNSI	RCATGY	3 cut(s)	427, 1600, 1981
BstSCI	CCNGG	3 cut(s)	277, 459, 862
BstSFI	CTRYAG	3 cut(s)	117, 123, 354
BstSLI	GKGCMC	2 cut(s)	577, 656
BstUI	CGCG	1 cut(s)	1464
BstV1I	GCAGC	8 cut(s)	516, 553, 710, 719, 793, 947, 1088, 2031
BstX2I	RGATCY	3 cut(s)	878, 1726, 1861
BstYI	RGATCY	3 cut(s)	878, 1726, 1861
BsuI	GTATCC	1 cut(s)	1841
BsuRI	GGCC	2 cut(s)	920, 1011
BtgI	CCRYGG	2 cut(s)	310, 1717
BtgZI	GCGATG	3 cut(s)	330, 813, 1445
BtsCI	GGATG	2 cut(s)	443, 902
BtsI	GCAGTG	2 cut(s)	656, 839
BtsIMutI	CAGTG	3 cut(s)	504, 656, 839
BtuMI	TCGCGA	1 cut(s)	1464
BveI	ACCTGC	1 cut(s)	571
Cac8I	GCNNGC	2 cut(s)	1477, 1944
CfoI	GCGC	2 cut(s)	269, 826
Cfr13I	GGNCC	1 cut(s)	560
Csp6I	GTAC	3 cut(s)	637, 994, 1648
CviQI	GTAC	3 cut(s)	637, 994, 1648
DinI	GGCGCC	1 cut(s)	268
DpnI	GATC	9 cut(s)	6, 17, 880, 911, 1467, 1551, 1728, 1863, 1999
DpnII	GATC	9 cut(s)	4, 15, 878, 909, 1465, 1549, 1726, 1861, 1997
EaeI	YGGCCR	1 cut(s)	918
Eam1104I	CTCTTC	2 cut(s)	542, 1169
EarI	CTCTTC	2 cut(s)	542, 1169
Eco130I	CCWWGG	2 cut(s)	673, 939
Eco31I	GGTCTC	1 cut(s)	1867
Eco47I	GGWCC	1 cut(s)	560
Eco57I	CTGAAG	5 cut(s)	126, 564, 566, 1229, 1305
EcoNI	CCTNNNNNAGG	1 cut(s)	861
EcoO109I	RGGNCCY	1 cut(s)	560
EcoRII	CCWGG	1 cut(s)	862
EcoT14I	CCWWGG	2 cut(s)	673, 939
EcoT22I	ATGCAT	1 cut(s)	1620
EgeI	GGCGCC	1 cut(s)	268
EheI	GGCGCC	1 cut(s)	268
ErhI	CCWWGG	2 cut(s)	673, 939
FaqI	GGGAC	4 cut(s)	549, 573, 1585, 1777
FauNDI	CATATG	1 cut(s)	1738
FbaI	TGATCA	2 cut(s)	909, 1549
Fnu4HI	GCNGC	9 cut(s)	527, 530, 567, 699, 708, 807, 961, 1077, 2020
FokI	GGATG	2 cut(s)	430, 889
Fsp4HI	GCNGC	9 cut(s)	527, 530, 567, 699, 708, 807, 961, 1077, 2020
FspBI	CTAG	5 cut(s)	674, 1185, 1413, 1698, 2016
GlaI	GCGC	2 cut(s)	268, 825
GluI	GCNGC	9 cut(s)	527, 530, 567, 699, 708, 807, 961, 1077, 2020
GsaI	CCCAGC	1 cut(s)	536
GsuI	CTGGAG	1 cut(s)	846
HaeII	RGCGCY	2 cut(s)	270, 827
HaeIII	GGCC	2 cut(s)	920, 1011
HapII	CCGG	2 cut(s)	279, 461
HhaI	GCGC	2 cut(s)	269, 826
Hin1I	GRCGYC	1 cut(s)	267
Hin6I	GCGC	2 cut(s)	267, 824
HinP1I	GCGC	2 cut(s)	267, 824
HincII	GTYRAC	1 cut(s)	338
HindII	GTYRAC	1 cut(s)	338
HindIII	AAGCTT	1 cut(s)	1241
HinfI	GANTC	6 cut(s)	369, 1043, 1300, 1420, 1439, 1784
HpaII	CCGG	2 cut(s)	279, 461
HphI	GGTGA	2 cut(s)	211, 313
Hpy166II	GTNNAC	9 cut(s)	292, 338, 575, 613, 639, 654, 1135, 1648, 1844
Hpy188III	TCNNGA	6 cut(s)	1185, 1436, 1463, 1809, 1856, 1995
Hpy8I	GTNNAC	9 cut(s)	292, 338, 575, 613, 639, 654, 1135, 1648, 1844
HpyAV	CCTTC	8 cut(s)	359, 457, 493, 588, 1109, 1280, 1566, 1754
HpyF10VI	GCNNNNNNNGC	6 cut(s)	390, 429, 572, 704, 707, 1939
Hsp92I	GRCGYC	1 cut(s)	267
HspAI	GCGC	2 cut(s)	267, 824
KasI	GGCGCC	1 cut(s)	266
Ksp22I	TGATCA	2 cut(s)	909, 1549
Kzo9I	GATC	9 cut(s)	4, 15, 878, 909, 1465, 1549, 1726, 1861, 1997
LguI	GCTCTTC	1 cut(s)	542
Lsp1109I	GCAGC	8 cut(s)	516, 553, 710, 719, 793, 947, 1088, 2031
LweI	GCATC	2 cut(s)	105, 988
MaeI	CTAG	5 cut(s)	674, 1185, 1413, 1698, 2016
MaeIII	GTNAC	6 cut(s)	319, 426, 871, 1592, 1678, 1910
MalI	GATC	9 cut(s)	6, 17, 880, 911, 1467, 1551, 1728, 1863, 1999
MboI	GATC	9 cut(s)	4, 15, 878, 909, 1465, 1549, 1726, 1861, 1997
MboII	GAAGA	8 cut(s)	478, 559, 839, 987, 1186, 1304, 1736, 1784
MfeI	CAATTG	2 cut(s)	933, 1068
MflI	RGATCY	3 cut(s)	878, 1726, 1861
MhlI	GDGCHC	2 cut(s)	577, 656
MlsI	TGGCCA	1 cut(s)	920
MluNI	TGGCCA	1 cut(s)	920
Mly113I	GGCGCC	1 cut(s)	267
MlyI	GAGTC	2 cut(s)	378, 1052
MmeI	TCCRAC	4 cut(s)	249, 1353, 1561, 1761
Mox20I	TGGCCA	1 cut(s)	920
Mph1103I	ATGCAT	1 cut(s)	1620
MroXI	GAANNNNTTC	2 cut(s)	593, 1299
MscI	TGGCCA	1 cut(s)	920
MseI	TTAA	7 cut(s)	192, 1098, 1215, 1449, 1664, 1733, 2040
MslI	CAYNNNNRTG	1 cut(s)	644
Msp20I	TGGCCA	1 cut(s)	920
MspA1I	CMGCKG	1 cut(s)	529
MspI	CCGG	2 cut(s)	279, 461
MspR9I	CCNGG	3 cut(s)	279, 461, 864
MunI	CAATTG	2 cut(s)	933, 1068
MvaI	CCWGG	1 cut(s)	864
MvnI	CGCG	1 cut(s)	1464
MwoI	GCNNNNNNNGC	6 cut(s)	390, 429, 572, 704, 707, 1939
NarI	GGCGCC	1 cut(s)	267
NciI	CCSGG	2 cut(s)	279, 461
NdeI	CATATG	1 cut(s)	1738
NdeII	GATC	9 cut(s)	4, 15, 878, 909, 1465, 1549, 1726, 1861, 1997
NlaIV	GGNNCC	3 cut(s)	268, 561, 1863
NmeAIII	GCCGAG	1 cut(s)	1423
NmuCI	GTSAC	1 cut(s)	319
NruI	TCGCGA	1 cut(s)	1464
NsiI	ATGCAT	1 cut(s)	1620
NspI	RCATGY	3 cut(s)	427, 1600, 1981
OliI	CACNNNNGTG	1 cut(s)	644
PciI	ACATGT	1 cut(s)	1596
PciSI	GCTCTTC	1 cut(s)	542
PdmI	GAANNNNTTC	2 cut(s)	593, 1299
PfeI	GAWTC	4 cut(s)	1300, 1420, 1439, 1784
PkrI	GCNGC	9 cut(s)	528, 531, 568, 700, 709, 808, 962, 1078, 2021
PleI	GAGTC	2 cut(s)	377, 1051
PluTI	GGCGCC	1 cut(s)	270
PpsI	GAGTC	2 cut(s)	377, 1051
PpuMI	RGGWCCY	1 cut(s)	560
PscI	ACATGT	1 cut(s)	1596
Psp5II	RGGWCCY	1 cut(s)	560
Psp6I	CCWGG	1 cut(s)	862
PspFI	CCCAGC	1 cut(s)	532
PspGI	CCWGG	1 cut(s)	862
PspN4I	GGNNCC	3 cut(s)	268, 561, 1863
PspPI	GGNCC	1 cut(s)	560
PspPPI	RGGWCCY	1 cut(s)	560
PsrI	GAACNNNNNNTAC	2 cut(s)	167, 199
PsuI	RGATCY	3 cut(s)	878, 1726, 1861
RruI	TCGCGA	1 cut(s)	1464
RsaI	GTAC	3 cut(s)	638, 995, 1649
RsaNI	GTAC	3 cut(s)	637, 994, 1648
RseI	CAYNNNNRTG	1 cut(s)	644
SapI	GCTCTTC	1 cut(s)	542
SaqAI	TTAA	7 cut(s)	192, 1098, 1215, 1449, 1664, 1733, 2040
SatI	GCNGC	9 cut(s)	527, 530, 567, 699, 708, 807, 961, 1077, 2020
Sau3AI	GATC	9 cut(s)	4, 15, 878, 909, 1465, 1549, 1726, 1861, 1997
Sau96I	GGNCC	1 cut(s)	560
SchI	GAGTC	2 cut(s)	378, 1052
ScrFI	CCNGG	3 cut(s)	279, 461, 864
SduI	GDGCHC	2 cut(s)	577, 656
SfaNI	GCATC	2 cut(s)	105, 988
SfcI	CTRYAG	3 cut(s)	117, 123, 354
SfoI	GGCGCC	1 cut(s)	268
SinI	GGWCC	1 cut(s)	560
SmiMI	CAYNNNNRTG	1 cut(s)	644
SmlI	CTYRAG	5 cut(s)	111, 388, 474, 882, 1557
SmoI	CTYRAG	5 cut(s)	111, 388, 474, 882, 1557
SpeI	ACTAGT	1 cut(s)	1697
SsiI	CCGC	2 cut(s)	517, 527
SspDI	GGCGCC	1 cut(s)	266
SspI	AATATT	1 cut(s)	1539
SspMI	CTAG	5 cut(s)	674, 1185, 1413, 1698, 2016
StyD4I	CCNGG	3 cut(s)	277, 459, 862
StyI	CCWWGG	2 cut(s)	673, 939
TaqI	TCGA	4 cut(s)	99, 668, 772, 2071
TatI	WGTACW	2 cut(s)	636, 1647
TauI	GCSGC	1 cut(s)	529
TfiI	GAWTC	4 cut(s)	1300, 1420, 1439, 1784
Tru1I	TTAA	7 cut(s)	192, 1098, 1215, 1449, 1664, 1733, 2040
Tru9I	TTAA	7 cut(s)	192, 1098, 1215, 1449, 1664, 1733, 2040
TscAI	CASTG	3 cut(s)	511, 656, 846
TseFI	GTSAC	1 cut(s)	319
TseI	GCWGC	8 cut(s)	529, 566, 698, 707, 806, 960, 1076, 2019
Tsp45I	GTSAC	1 cut(s)	319
TspDTI	ATGAA	9 cut(s)	40, 288, 511, 792, 1041, 1326, 1517, 1830, 2058
TspRI	CASTG	3 cut(s)	511, 656, 846
VneI	GTGCAC	2 cut(s)	573, 652
VpaK11BI	GGWCC	1 cut(s)	560
XagI	CCTNNNNNAGG	1 cut(s)	861
XapI	RAATTY	3 cut(s)	593, 1211, 1255
XbaI	TCTAGA	1 cut(s)	1184
XceI	RCATGY	3 cut(s)	427, 1600, 1981
XmaJI	CCTAGG	1 cut(s)	673
XmnI	GAANNNNTTC	2 cut(s)	593, 1299
XspI	CTAG	5 cut(s)	674, 1185, 1413, 1698, 2016
Zsp2I	ATGCAT	1 cut(s)	1620

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.